pycom02g18890
ERF Family

Belongs to the protein kinase superfamily

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr2
OG0000416
Physical Location & Seq
Forward (+)
16949920 .. 16953463
3544 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom02g18890.13

Sequence Viewer

Length: 2217 bp
ATGTGCATTGAAAAAATCTGTTACATGAGTAAGATGATGTCCAATAAACTCCAAAGCACGAGCTTCAATTTAACTATGAATTTTGGTCAATTTTTGAGTTCTCATTTGTCCTCGAGTCACAGTCCCAGCAGACTCATGGGTCTCTTTGCATACTTTCCCAAGATATGTACTTGCTTAAAAACAAGCAGGAGCTCCTTTCAAATTTTAATTGTTCAATTTTTTTGGACATTTGTCAAATTGTTATTGTATGAAAGCAGGAGTTCCTGCTTATTAAGCAGCAAAGCTTTCTCCGCATCCGCATGGCCATTACCCTATTCCAAAACTTCAACCACGCAACGAAAAATGTCAGATAGTGAAGTTGTGAAGGGGTATTATTGTTTGATGCATTCAATTTATGAAACGATCTTTTATTCAAATAATTTTCTTTTTGTAAAAATGATCTTTAAGAATATAAGTAAGTACCATAAAAAAATACAAGTTAGCTTTTATGGTACAATACTCTCATTAAAAAATAACAACAATATTCTAATACTACTCTGGTCTAATCTCTTGGGTGTGGTGGAATTCATCATATTCTTCTTTTTGGGTTTGACTATATATCTATGGCGTAGATATTTGGACAGATTCAGTTTCGTGTCAAGCTGTGTGAGAGAGAGATGTGAGTTTCTTCTGCAACTACTACTTCTATATATTATTGTTGTCAACTTCAAAATAAGGCTTTGCCAACCACCTACTTCATTATTCACATACAGAAAAAAAAAAGAAAGAGAAAGCCAAGCAAGAAAAGAAATCTGTGCGGTTCTCTCCCAACCCTCTCAACCTCCCAACCATTTTCAAAATATTGGAGAAATAATTGACAGAGCTGGGTTTAATTTTCTACAAACGATTCAATGGCAGGCAGAGGGAATTGCTGTAGGATTCTATACAATGAAGTGCTTCTACTATTTCAAGGACAAGGCTAGGAGCAGGGAACAAAGATCTGCTCCAGAACTGAAAGAACAAACAAAATCGGATTATTCAGGTGGCAGCGATAGGGTCATTAAGTCCACCTGTTCCGTGAATTCACCCCGCGGTATACCAGAAATGTACGAAGAGAAGGCTCATAATTTGCTGGTGTTTACATTCACGGAGCTCAGGCAGGCAACAAACGACTTCAGTAGGCTGCTTAAGATCGGAGAAGGCGGATTTGGGAATGTATACAAGGGTTCAATTAAGCCCGCTGATGACAAGGGCCGCGATCCAGTCATTGTCGCCATTAAAAAGCTTAACAATGATGGCTTACAGGGTCACAAGCAATGGGTGGCAGAAGTTCAATTTTTGAGTGTTGTGGAGCACCCGAATCTTGTCAAACTAATCGGATACTGTGCTGTTGATGGAGAAAGAGGGATTCAACGACTGCTTGTCTATGAATTCATGCCCAACAGAAGTTTAGAAGATCATCTATTCAACAAGGTGTACTCAACCGTGCCTTGGGAGACAAGGTTACAGATAATACTTGGAGCAGCTCAAGGATTGGCGTATCTGCACGAAGGATTGGAAGTTCAGGTAATCTATCGAGATTTCAAATGCGCGAATGTGTTACTGGATGAGAACTTTAAACCGAAGCTTTCGGACTTCGGGCTTGCTAGGGAGGGGCCAATGGTTGGCCATACTCATGTTTCAACTGCAGTAATGGGAACTTACGGGTACGCTGCTCCAGATTACATCGAGACAGGTCATCTCACAACTAAGAGTGATGTCTGGAGTTTCGGTGTGGTGCTGTATGAGATTCTTACAGGCAGACGATCATTGGAGAGAAACAGGCCAAGAACAGAGCAGAAACTTTTGGAATGGGTGAAACAGTTCCCTCCTGATGGCAAAAAGTTTGGCTTGATGATGGATAGCAGGCTGGAAAACAAGTATTCTATCACTGCGGCAAGAAAGATCGCTAAGCTGGCCGAAAGTTGCCTGTCAAAGAGCGCAAAAGATCGGCCAACAATGAGTCAGGTAGTTGACAAACTGAAACAGATTATTCAAGACTCCGAAGAGGGAATCCCATCCGAGAGAAGTCCTGAATCCGTTGAGAATGACCACGCCGACTCAGTAACAAAGCCAAGACAGTTGGAGGCCTCAGAGTCATGGCAGAGACGAATGGCTCACCTGGCCAAACTAGGCGAGCATGTGGGGAGCGCTAGTAGAGGAAGATTCATGATTATGCAGAGGGCAAAGGTGACGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Pkinase Pkinase_Tyr

Protein Analysis

739

Amino Acids

84.67

Weight (kDa)

9.38

Isoelectric Point (pI)

43.62

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000416)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G47070
fragaria_vesca FvH4_5g20770 FvH4_5g20770 FvH4_5g20770 FvH4_5g20770 FvH4_7g08930 FvH4_7g08930 FvH4_7g08930 FvH4_7g08930 FvH4_7g08930
malus_domestica MD02G1222400.v1.1 MD04G1053100.v1.1 MD06G1044400.v1.1 MD07G1093200.v1.1
prunus_persica Prupe.2G118500_v2.0.a1 Prupe.2G118500_v2.0.a1 Prupe.2G118500_v2.0.a1 Prupe.2G118500_v2.0.a1 Prupe.2G118500_v2.0.a1 Prupe.5G052200_v2.0.a1 Prupe.5G052200_v2.0.a1
pyrus_communis pycom02g18890 pycom04g04660 pycom06g03780 pycom07g07640
rosa_chinensis RchiOBHm_Chr0c22g0500461 RchiOBHm_Chr0c22g0500491 RchiOBHm_Chr0c22g0500531 RchiOBHm_Chr0c22g0500541 RchiOBHm_Chr0c22g0500641 RchiOBHm_Chr0c22g0500691 RchiOBHm_Chr1g0344381 RchiOBHm_Chr6g0259661 RchiOBHm_Chr6g0259701 RchiOBHm_Chr7g0205471 RchiOBHm_Chr7g0206561 RchiOBHm_Chr7g0206611 RchiOBHm_Chr7g0206731 RchiOBHm_Chr7g0206741 RchiOBHm_Chr7g0206781 RchiOBHm_Chr7g0206801 RchiOBHm_Chr7g0206811 RchiOBHm_Chr7g0206831 RchiOBHm_Chr7g0206861 RchiOBHm_Chr7g0206891 RchiOBHm_Chr7g0206981 RchiOBHm_Chr7g0206991 RchiOBHm_Chr7g0207071 RchiOBHm_Chr7g0207121 RchiOBHm_Chr7g0207151 RchiOBHm_Chr7g0207181 RchiOBHm_Chr7g0207241 RchiOBHm_Chr7g0207251 RchiOBHm_Chr7g0207261 RchiOBHm_Chr7g0207411 RchiOBHm_Chr7g0207471 RchiOBHm_Chr7g0207701
rosa_laevigata RLG00000003296 RLG00000003302 RLG00000003326 RLG00000003346 RLG00000028901
rosa_multiflora Rmu_sc0000536.1_g000001 Rmu_sc0000536.1_g000002 Rmu_sc0004987.1_g000003 Rmu_sc0008509.1_g000002 Rmu_sc0008509.1_g000029 Rmu_sc0012558.1_g000001 Rmu_sc0013160.1_g000002 Rmu_sc0014912.1_g000004
rosa_roxburghii Rroxscaffold_3G00251310 Rroxscaffold_3G00251320 Rroxscaffold_3G00251410 Rroxscaffold_3G00252190 Rroxscaffold_4G00309810 Rroxscaffold_7G00206970 Rroxscaffold_7G00207090
rosa_rugosa Rorug01G0173500 Rorug01G0173600 Rorug05G0590200 Rorug07G0096400 Rorug07G0096700 Rorug07G0096900 Rorug07G0098500 Rorug07G0099000
rosa_samantha Rh1AG189700 Rh1BG156700 Rh1DG188100 Rh7BG221600 Rh7BG225000 Rh7BG225300 Rh7BG227500 Rh7BG227700 Rh7CG244800
rosa_wichuraiana Rw1G015650 Rw6G009210 Rw7G019820 Rw7G019850

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 138
AccB7I CCANNNNNTGG 1 cut(s) 1643
AccI GTMKAC 2 cut(s) 1075, 1197
AccII CGCG 3 cut(s) 1071, 1236, 1571
AciI CCGC 9 cut(s) 291, 297, 797, 1069, 1071, 1182, 1218, 1234, 1913
AclWI GGATC 1 cut(s) 1232
AcoI YGGCCR 5 cut(s) 302, 1645, 1935, 1970, 2142
AcsI RAATTY 5 cut(s) 79, 201, 563, 1060, 1409
AcuI CTGAAG 1 cut(s) 1138
AfaI GTAC 6 cut(s) 169, 461, 493, 1088, 1457, 1688
AfeI AGCGCT 1 cut(s) 2170
AfiI CCNNNNNNNGG 3 cut(s) 1470, 1643, 1853
AflII CTTAAG 1 cut(s) 1166
AhdI GACNNNNNGTC 1 cut(s) 1400
AjiI CACGTC 1 cut(s) 2214
AjnI CCWGG 1 cut(s) 2139
AjuI GAANNNNNNNTTGG 2 cut(s) 1170, 1202
AloI GAACNNNNNNTCC 2 cut(s) 1524, 1556
Alw21I GWGCWC 3 cut(s) 194, 1134, 1335
Alw26I GTCTC 4 cut(s) 146, 1469, 1703, 2119
AlwI GGATC 1 cut(s) 1232
Ama87I CYCGRG 1 cut(s) 112
Aor51HI AGCGCT 1 cut(s) 2170
AoxI GGCC 9 cut(s) 302, 1231, 1634, 1645, 1802, 1935, 1970, 2106, 2142
ApeKI GCWGC 5 cut(s) 276, 1026, 1162, 1502, 1691
ApoI RAATTY 5 cut(s) 79, 201, 563, 1060, 1409
Asp700I GAANNNNTTC 2 cut(s) 935, 1841
AspLEI GCGC 3 cut(s) 1571, 1961, 2171
AspS9I GGNCC 2 cut(s) 1231, 1634
AsuHPI GGTGA 3 cut(s) 1056, 1846, 2129
AvaI CYCGRG 1 cut(s) 112
BalI TGGCCA 3 cut(s) 304, 1647, 2144
BanII GRGCYC 2 cut(s) 194, 1134
BarI GAAGNNNNNNTAC 2 cut(s) 923, 955
BauI CACGAG 1 cut(s) 58
Bbv12I GWGCWC 3 cut(s) 194, 1134, 1335
BbvI GCAGC 5 cut(s) 288, 1038, 1149, 1514, 1678
BccI CCATC 5 cut(s) 1268, 1367, 1847, 1870, 2044
BciT130I CCWGG 1 cut(s) 2141
BciVI GTATCC 1 cut(s) 1352
BcoDI GTCTC 4 cut(s) 146, 1469, 1703, 2119
BfaI CTAG 4 cut(s) 960, 1626, 2150, 2172
BfmI CTRYAG 2 cut(s) 912, 1665
BfoI RGCGCY 1 cut(s) 2172
BfrI CTTAAG 1 cut(s) 1166
BfuI GTATCC 1 cut(s) 1352
BglII AGATCT 1 cut(s) 977
BisI GCNGC 7 cut(s) 277, 1027, 1163, 1234, 1503, 1692, 1914
BlpI GCTNAGC 1 cut(s) 1929
BlsI GCNGC 7 cut(s) 278, 1028, 1164, 1235, 1504, 1693, 1915
Bme1390I CCNGG 1 cut(s) 2141
BmeRI GACNNNNNGTC 1 cut(s) 1400
BmeT110I CYCGRG 1 cut(s) 112
BmgBI CACGTC 1 cut(s) 2214
BmgT120I GGNCC 2 cut(s) 1231, 1634
BmiI GGNNCC 1 cut(s) 1635
BmrFI CCNGG 1 cut(s) 2141
BmsI GCATC 2 cut(s) 302, 372
BoxI GACNNNNGTC 1 cut(s) 230
BpmI CTGGAG 3 cut(s) 969, 1680, 1762
Bpu10I CCTNAGC 1 cut(s) 1133
Bpu1102I GCTNAGC 1 cut(s) 1929
BpuEI CTTGAG 1 cut(s) 1491
BsaI GGTCTC 1 cut(s) 146
BsaJI CCNNGG 2 cut(s) 1069, 1469
BsaXI ACNNNNNCTCC 6 cut(s) 1466, 1496, 1735, 1765, 2158, 2188
Bsc4I CCNNNNNNNGG 3 cut(s) 1470, 1643, 1853
Bse1I ACTGG 2 cut(s) 1241, 1587
Bse3DI GCAATG 1 cut(s) 1301
BseBI CCWGG 1 cut(s) 2141
BseDI CCNNGG 2 cut(s) 1069, 1469
BseGI GGATG 3 cut(s) 293, 1591, 2036
BseLI CCNNNNNNNGG 3 cut(s) 1470, 1643, 1853
BseMI GCAATG 1 cut(s) 1301
BseMII CTCAG 3 cut(s) 1147, 2094, 2124
BseNI ACTGG 2 cut(s) 1241, 1587
BseXI GCAGC 5 cut(s) 288, 1038, 1149, 1514, 1678
BseYI CCCAGC 2 cut(s) 125, 863
BsgI GTGCAG 1 cut(s) 1508
Bsh1236I CGCG 3 cut(s) 1071, 1236, 1571
BshFI GGCC 9 cut(s) 304, 1233, 1636, 1647, 1804, 1937, 1972, 2108, 2144
BsiHKAI GWGCWC 3 cut(s) 194, 1134, 1335
BsiHKCI CYCGRG 1 cut(s) 112
BslFI GGGAC 1 cut(s) 108
BslI CCNNNNNNNGG 3 cut(s) 1470, 1643, 1853
BsmAI GTCTC 4 cut(s) 146, 1469, 1703, 2119
BsmBI CGTCTC 1 cut(s) 2119
BsmFI GGGAC 1 cut(s) 108
BsmI GAATGC 1 cut(s) 385
BsnI GGCC 9 cut(s) 304, 1233, 1636, 1647, 1804, 1937, 1972, 2108, 2144
Bso31I GGTCTC 1 cut(s) 146
BsoBI CYCGRG 1 cut(s) 112
Bsp1286I GDGCHC 3 cut(s) 194, 1134, 1335
Bsp143I GATC 9 cut(s) 402, 438, 977, 1170, 1237, 1435, 1784, 1923, 1966
Bsp1720I GCTNAGC 1 cut(s) 1929
BspACI CCGC 9 cut(s) 291, 297, 797, 1069, 1071, 1182, 1218, 1234, 1913
BspANI GGCC 9 cut(s) 304, 1233, 1636, 1647, 1804, 1937, 1972, 2108, 2144
BspCNI CTCAG 3 cut(s) 1146, 2093, 2123
BspFNI CGCG 3 cut(s) 1071, 1236, 1571
BspHI TCATGA 1 cut(s) 2187
BspLI GGNNCC 1 cut(s) 1635
BspMAI CTGCAG 1 cut(s) 1669
BspPI GGATC 1 cut(s) 1232
BspTI CTTAAG 1 cut(s) 1166
BspTNI GGTCTC 1 cut(s) 146
BsrDI GCAATG 1 cut(s) 1301
BsrI ACTGG 2 cut(s) 1241, 1587
BssECI CCNNGG 2 cut(s) 1069, 1469
BssMI GATC 9 cut(s) 402, 438, 977, 1170, 1237, 1435, 1784, 1923, 1966
BssNAI GTATAC 2 cut(s) 1076, 1198
BssSI CACGAG 1 cut(s) 58
BssT1I CCWWGG 1 cut(s) 1469
Bst1107I GTATAC 2 cut(s) 1076, 1198
Bst2BI CACGAG 1 cut(s) 58
Bst2UI CCWGG 1 cut(s) 2141
Bst4CI ACNGT 5 cut(s) 122, 1364, 1465, 1842, 2100
Bst6I CTCTTC 2 cut(s) 1086, 2019
BstAFI CTTAAG 1 cut(s) 1166
BstC8I GCNNGC 7 cut(s) 897, 1140, 1218, 1623, 1886, 1935, 2156
BstDEI CTNAG 5 cut(s) 1133, 1728, 1929, 2080, 2110
BstDSI CCRYGG 1 cut(s) 1069
BstF5I GGATG 3 cut(s) 293, 1591, 2036
BstFNI CGCG 3 cut(s) 1071, 1236, 1571
BstH2I RGCGCY 1 cut(s) 2172
BstHHI GCGC 3 cut(s) 1571, 1961, 2171
BstKTI GATC 9 cut(s) 405, 441, 980, 1173, 1240, 1438, 1787, 1926, 1969
BstMAI GTCTC 4 cut(s) 146, 1469, 1703, 2119
BstMBI GATC 9 cut(s) 402, 438, 977, 1170, 1237, 1435, 1784, 1923, 1966
BstMWI GCNNNNNNNGC 4 cut(s) 273, 290, 1934, 2141
BstNI CCWGG 1 cut(s) 2141
BstNSI RCATGY 1 cut(s) 2162
BstPAI GACNNNNGTC 1 cut(s) 230
BstSCI CCNGG 1 cut(s) 2139
BstSFI CTRYAG 2 cut(s) 912, 1665
BstUI CGCG 3 cut(s) 1071, 1236, 1571
BstV1I GCAGC 5 cut(s) 288, 1038, 1149, 1514, 1678
BstX2I RGATCY 1 cut(s) 977
BstYI RGATCY 1 cut(s) 977
BstZ17I GTATAC 2 cut(s) 1076, 1198
BsuI GTATCC 1 cut(s) 1352
BsuRI GGCC 9 cut(s) 304, 1233, 1636, 1647, 1804, 1937, 1972, 2108, 2144
BtgI CCRYGG 1 cut(s) 1069
BtrI CACGTC 1 cut(s) 2214
BtsCI GGATG 3 cut(s) 293, 1591, 2036
BtsI GCAGTG 1 cut(s) 1908
BtsIMutI CAGTG 1 cut(s) 1908
Cac8I GCNNGC 7 cut(s) 897, 1140, 1218, 1623, 1886, 1935, 2156
CciI TCATGA 1 cut(s) 2187
CfoI GCGC 3 cut(s) 1571, 1961, 2171
Cfr13I GGNCC 2 cut(s) 1231, 1634
Cfr42I CCGCGG 1 cut(s) 1072
Csp6I GTAC 6 cut(s) 168, 460, 492, 1087, 1456, 1687
CviAII CATG 8 cut(s) 25, 136, 300, 1414, 1655, 2118, 2159, 2188
CviQI GTAC 6 cut(s) 168, 460, 492, 1087, 1456, 1687
DdeI CTNAG 5 cut(s) 1133, 1728, 1929, 2080, 2110
DpnI GATC 9 cut(s) 404, 440, 979, 1172, 1239, 1437, 1786, 1925, 1968
DpnII GATC 9 cut(s) 402, 438, 977, 1170, 1237, 1435, 1784, 1923, 1966
DraI TTTAAA 1 cut(s) 1597
DrdI GACNNNNNNGTC 1 cut(s) 138
DriI GACNNNNNGTC 1 cut(s) 1400
DseDI GACNNNNNNGTC 1 cut(s) 138
EaeI YGGCCR 5 cut(s) 302, 1645, 1935, 1970, 2142
Eam1104I CTCTTC 2 cut(s) 1086, 2019
Eam1105I GACNNNNNGTC 1 cut(s) 1400
EarI CTCTTC 2 cut(s) 1086, 2019
EciI GGCGGA 1 cut(s) 1197
Ecl136II GAGCTC 2 cut(s) 192, 1132
Eco130I CCWWGG 1 cut(s) 1469
Eco147I AGGCCT 1 cut(s) 2108
Eco24I GRGCYC 2 cut(s) 194, 1134
Eco31I GGTCTC 1 cut(s) 146
Eco47III AGCGCT 1 cut(s) 2170
Eco53kI GAGCTC 2 cut(s) 192, 1132
Eco57I CTGAAG 1 cut(s) 1138
Eco88I CYCGRG 1 cut(s) 112
EcoICRI GAGCTC 2 cut(s) 192, 1132
EcoRI GAATTC 3 cut(s) 563, 1060, 1409
EcoRII CCWGG 1 cut(s) 2139
EcoT14I CCWWGG 1 cut(s) 1469
EcoT22I ATGCAT 1 cut(s) 387
EcoT38I GRGCYC 2 cut(s) 194, 1134
ErhI CCWWGG 1 cut(s) 1469
Esp3I CGTCTC 1 cut(s) 2119
FaeI CATG 8 cut(s) 28, 139, 303, 1417, 1658, 2121, 2162, 2191
FalI AAGNNNNNCTT 2 cut(s) 1853, 1885
FaqI GGGAC 1 cut(s) 108
FatI CATG 8 cut(s) 24, 135, 299, 1413, 1654, 2117, 2158, 2187
FauI CCCGC 2 cut(s) 1076, 1225
FblI GTMKAC 2 cut(s) 1075, 1197
Fnu4HI GCNGC 7 cut(s) 277, 1027, 1163, 1234, 1503, 1692, 1914
FokI GGATG 3 cut(s) 280, 1598, 2023
FriOI GRGCYC 2 cut(s) 194, 1134
Fsp4HI GCNGC 7 cut(s) 277, 1027, 1163, 1234, 1503, 1692, 1914
FspBI CTAG 4 cut(s) 960, 1626, 2150, 2172
GlaI GCGC 3 cut(s) 1570, 1960, 2170
GluI GCNGC 7 cut(s) 277, 1027, 1163, 1234, 1503, 1692, 1914
GsaI CCCAGC 2 cut(s) 129, 867
GsuI CTGGAG 3 cut(s) 969, 1680, 1762
HaeII RGCGCY 1 cut(s) 2172
HaeIII GGCC 9 cut(s) 304, 1233, 1636, 1647, 1804, 1937, 1972, 2108, 2144
HhaI GCGC 3 cut(s) 1571, 1961, 2171
Hin1II CATG 8 cut(s) 28, 139, 303, 1417, 1658, 2121, 2162, 2191
Hin6I GCGC 3 cut(s) 1569, 1959, 2169
HinP1I GCGC 3 cut(s) 1569, 1959, 2169
HincII GTYRAC 2 cut(s) 703, 1993
HindII GTYRAC 2 cut(s) 703, 1993
HindIII AAGCTT 3 cut(s) 282, 1262, 1604
HphI GGTGA 3 cut(s) 1056, 1846, 2129
Hpy166II GTNNAC 7 cut(s) 703, 1047, 1076, 1119, 1198, 1456, 1993
Hpy188I TCNGA 8 cut(s) 349, 1012, 1175, 1358, 1612, 2023, 2041, 2113
Hpy188III TCNNGA 9 cut(s) 986, 1556, 1697, 1708, 1741, 1850, 2015, 2051, 2188
Hpy8I GTNNAC 7 cut(s) 703, 1047, 1076, 1119, 1198, 1456, 1993
HpyAV CCTTC 4 cut(s) 358, 1090, 1172, 1523
HpyCH4III ACNGT 5 cut(s) 122, 1364, 1465, 1842, 2100
HpyCH4IV ACGT 1 cut(s) 2213
HpyCH4V TGCA 7 cut(s) 6, 149, 385, 673, 1525, 1667, 2197
HpyF10VI GCNNNNNNNGC 4 cut(s) 273, 290, 1934, 2141
HpyF3I CTNAG 5 cut(s) 1133, 1728, 1929, 2080, 2110
HpySE526I ACGT 1 cut(s) 2213
Hsp92II CATG 8 cut(s) 28, 139, 303, 1417, 1658, 2121, 2162, 2191
HspAI GCGC 3 cut(s) 1569, 1959, 2169
KspI CCGCGG 1 cut(s) 1072
Kzo9I GATC 9 cut(s) 402, 438, 977, 1170, 1237, 1435, 1784, 1923, 1966
LmnI GCTCC 9 cut(s) 189, 197, 963, 988, 1129, 1330, 1499, 1699, 2166
Lsp1109I GCAGC 5 cut(s) 288, 1038, 1149, 1514, 1678
LweI GCATC 2 cut(s) 302, 372
MaeI CTAG 4 cut(s) 960, 1626, 2150, 2172
MaeII ACGT 1 cut(s) 2213
MaeIII GTNAC 7 cut(s) 20, 116, 1286, 1482, 1578, 2083, 2209
MalI GATC 9 cut(s) 404, 440, 979, 1172, 1239, 1437, 1786, 1925, 1968
MboI GATC 9 cut(s) 402, 438, 977, 1170, 1237, 1435, 1784, 1923, 1966
MboII GAAGA 6 cut(s) 568, 659, 1103, 1445, 2036, 2193
MflI RGATCY 1 cut(s) 977
MhlI GDGCHC 3 cut(s) 194, 1134, 1335
MlsI TGGCCA 3 cut(s) 304, 1647, 2144
MluNI TGGCCA 3 cut(s) 304, 1647, 2144
MlyI GAGTC 6 cut(s) 124, 126, 1990, 2012, 2072, 2123
MmeI TCCRAC 1 cut(s) 2082
Mox20I TGGCCA 3 cut(s) 304, 1647, 2144
Mph1103I ATGCAT 1 cut(s) 387
MroXI GAANNNNTTC 2 cut(s) 935, 1841
MscI TGGCCA 3 cut(s) 304, 1647, 2144
MslI CAYNNNNRTG 3 cut(s) 298, 1653, 2192
Msp20I TGGCCA 3 cut(s) 304, 1647, 2144
MspA1I CMGCKG 2 cut(s) 1071, 1220
MspCI CTTAAG 1 cut(s) 1166
MspR9I CCNGG 1 cut(s) 2141
Mva1269I GAATGC 1 cut(s) 385
MvaI CCWGG 1 cut(s) 2141
MvnI CGCG 3 cut(s) 1071, 1236, 1571
MwoI GCNNNNNNNGC 4 cut(s) 273, 290, 1934, 2141
NdeII GATC 9 cut(s) 402, 438, 977, 1170, 1237, 1435, 1784, 1923, 1966
NlaIII CATG 8 cut(s) 28, 139, 303, 1417, 1658, 2121, 2162, 2191
NlaIV GGNNCC 1 cut(s) 1635
NmuCI GTSAC 3 cut(s) 116, 1286, 2209
NsiI ATGCAT 1 cut(s) 387
NspI RCATGY 1 cut(s) 2162
PaeR7I CTCGAG 1 cut(s) 112
PagI TCATGA 1 cut(s) 2187
PceI AGGCCT 1 cut(s) 2108
PctI GAATGC 1 cut(s) 385
PdmI GAANNNNTTC 2 cut(s) 935, 1841
PfeI GAWTC 9 cut(s) 624, 886, 918, 1339, 1387, 1768, 2031, 2054, 2184
PflMI CCANNNNNTGG 1 cut(s) 1643
PkrI GCNGC 7 cut(s) 278, 1028, 1164, 1235, 1504, 1693, 1915
PleI GAGTC 6 cut(s) 123, 126, 1989, 2012, 2072, 2122
PpsI GAGTC 6 cut(s) 123, 126, 1989, 2012, 2072, 2122
PshAI GACNNNNGTC 1 cut(s) 230
Psp124BI GAGCTC 2 cut(s) 194, 1134
Psp6I CCWGG 1 cut(s) 2139
PspFI CCCAGC 2 cut(s) 125, 863
PspGI CCWGG 1 cut(s) 2139
PspN4I GGNNCC 1 cut(s) 1635
PspPI GGNCC 2 cut(s) 1231, 1634
PspXI VCTCGAGB 1 cut(s) 112
PstI CTGCAG 1 cut(s) 1669
PsuI RGATCY 1 cut(s) 977
RsaI GTAC 6 cut(s) 169, 461, 493, 1088, 1457, 1688
RsaNI GTAC 6 cut(s) 168, 460, 492, 1087, 1456, 1687
RseI CAYNNNNRTG 3 cut(s) 298, 1653, 2192
SacI GAGCTC 2 cut(s) 194, 1134
SacII CCGCGG 1 cut(s) 1072
SatI GCNGC 7 cut(s) 277, 1027, 1163, 1234, 1503, 1692, 1914
Sau3AI GATC 9 cut(s) 402, 438, 977, 1170, 1237, 1435, 1784, 1923, 1966
Sau96I GGNCC 2 cut(s) 1231, 1634
SchI GAGTC 6 cut(s) 124, 126, 1990, 2012, 2072, 2123
ScrFI CCNGG 1 cut(s) 2141
SduI GDGCHC 3 cut(s) 194, 1134, 1335
SfaNI GCATC 2 cut(s) 302, 372
SfcI CTRYAG 2 cut(s) 912, 1665
Sfr274I CTCGAG 1 cut(s) 112
Sfr303I CCGCGG 1 cut(s) 1072
SgrBI CCGCGG 1 cut(s) 1072
SlaI CTCGAG 1 cut(s) 112
SmiMI CAYNNNNRTG 3 cut(s) 298, 1653, 2192
SmlI CTYRAG 3 cut(s) 112, 1166, 1506
SmoI CTYRAG 3 cut(s) 112, 1166, 1506
SseBI AGGCCT 1 cut(s) 2108
SsiI CCGC 9 cut(s) 291, 297, 797, 1069, 1071, 1182, 1218, 1234, 1913
SspI AATATT 2 cut(s) 523, 841
SspMI CTAG 4 cut(s) 960, 1626, 2150, 2172
SstI GAGCTC 2 cut(s) 194, 1134
StuI AGGCCT 1 cut(s) 2108
StyD4I CCNGG 1 cut(s) 2139
StyI CCWWGG 1 cut(s) 1469
TaaI ACNGT 5 cut(s) 122, 1364, 1465, 1842, 2100
TaiI ACGT 1 cut(s) 2216
TaqI TCGA 3 cut(s) 113, 1555, 1707
TatI WGTACW 2 cut(s) 167, 1455
TauI GCSGC 2 cut(s) 1236, 1916
TfiI GAWTC 9 cut(s) 624, 886, 918, 1339, 1387, 1768, 2031, 2054, 2184
TscAI CASTG 1 cut(s) 1915
TseFI GTSAC 3 cut(s) 116, 1286, 2209
TseI GCWGC 5 cut(s) 276, 1026, 1162, 1502, 1691
Tsp45I GTSAC 3 cut(s) 116, 1286, 2209
TspDTI ATGAA 9 cut(s) 92, 264, 411, 556, 726, 944, 1402, 1422, 2176
TspGWI ACGGA 3 cut(s) 1045, 1142, 2047
TspRI CASTG 1 cut(s) 1915
Van91I CCANNNNNTGG 1 cut(s) 1643
Vha464I CTTAAG 1 cut(s) 1166
XapI RAATTY 5 cut(s) 79, 201, 563, 1060, 1409
XceI RCATGY 1 cut(s) 2162
XcmI CCANNNNNNNNNTGG 1 cut(s) 133
XhoI CTCGAG 1 cut(s) 112
XmiI GTMKAC 2 cut(s) 1075, 1197
XmnI GAANNNNTTC 2 cut(s) 935, 1841
XspI CTAG 4 cut(s) 960, 1626, 2150, 2172
Zsp2I ATGCAT 1 cut(s) 387
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.