Belongs to the protein kinase superfamily
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AccI | GTMKAC | 1 cut(s) | 145 |
| AccII | CGCG | 2 cut(s) | 139, 141 |
| AciI | CCGC | 2 cut(s) | 137, 141 |
| AclWI | GGATC | 1 cut(s) | 299 |
| AcsI | RAATTY | 1 cut(s) | 130 |
| AflII | CTTAAG | 1 cut(s) | 236 |
| AgsI | TTSAA | 1 cut(s) | 279 |
| AjuI | GAANNNNNNNTTGG | 2 cut(s) | 240, 272 |
| AluBI | AGCT | 5 cut(s) | 56, 64, 81, 98, 202 |
| AluI | AGCT | 5 cut(s) | 56, 64, 81, 98, 202 |
| AlwI | GGATC | 1 cut(s) | 299 |
| AlwNI | CAGNNNCTG | 2 cut(s) | 59, 98 |
| ApeKI | GCWGC | 1 cut(s) | 78 |
| ApoI | RAATTY | 1 cut(s) | 130 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 8 |
| AspLEI | GCGC | 1 cut(s) | 141 |
| AsuHPI | GGTGA | 1 cut(s) | 126 |
| BarI | GAAGNNNNNNTAC | 1 cut(s) | 28 |
| BbsI | GAAGAC | 1 cut(s) | 108 |
| BbvCI | CCTCAGC | 1 cut(s) | 203 |
| BbvI | GCAGC | 1 cut(s) | 90 |
| BfrI | CTTAAG | 1 cut(s) | 236 |
| BisI | GCNGC | 1 cut(s) | 79 |
| BlpI | GCTNAGC | 1 cut(s) | 124 |
| BlsI | GCNGC | 1 cut(s) | 80 |
| BpiI | GAAGAC | 1 cut(s) | 108 |
| Bpu10I | CCTNAGC | 2 cut(s) | 60, 203 |
| Bpu1102I | GCTNAGC | 1 cut(s) | 124 |
| BsaJI | CCNNGG | 1 cut(s) | 297 |
| Bse1I | ACTGG | 1 cut(s) | 308 |
| BseDI | CCNNGG | 1 cut(s) | 297 |
| BseMII | CTCAG | 4 cut(s) | 51, 138, 194, 282 |
| BseNI | ACTGG | 1 cut(s) | 308 |
| BseXI | GCAGC | 1 cut(s) | 90 |
| Bsh1236I | CGCG | 2 cut(s) | 139, 141 |
| Bsp143I | GATC | 2 cut(s) | 50, 304 |
| Bsp1720I | GCTNAGC | 1 cut(s) | 124 |
| BspACI | CCGC | 2 cut(s) | 137, 141 |
| BspCNI | CTCAG | 4 cut(s) | 52, 137, 195, 283 |
| BspFNI | CGCG | 2 cut(s) | 139, 141 |
| BspPI | GGATC | 1 cut(s) | 299 |
| BspTI | CTTAAG | 1 cut(s) | 236 |
| BsrI | ACTGG | 1 cut(s) | 308 |
| BssECI | CCNNGG | 1 cut(s) | 297 |
| BssMI | GATC | 2 cut(s) | 50, 304 |
| BssNAI | GTATAC | 1 cut(s) | 146 |
| BssT1I | CCWWGG | 1 cut(s) | 297 |
| Bst1107I | GTATAC | 1 cut(s) | 146 |
| BstAFI | CTTAAG | 1 cut(s) | 236 |
| BstC8I | GCNNGC | 2 cut(s) | 210, 288 |
| BstDEI | CTNAG | 5 cut(s) | 33, 60, 124, 203, 291 |
| BstFNI | CGCG | 2 cut(s) | 139, 141 |
| BstHHI | GCGC | 1 cut(s) | 141 |
| BstKTI | GATC | 2 cut(s) | 53, 307 |
| BstMBI | GATC | 2 cut(s) | 50, 304 |
| BstUI | CGCG | 2 cut(s) | 139, 141 |
| BstV1I | GCAGC | 1 cut(s) | 90 |
| BstV2I | GAAGAC | 1 cut(s) | 108 |
| BstZ17I | GTATAC | 1 cut(s) | 146 |
| Cac8I | GCNNGC | 2 cut(s) | 210, 288 |
| CaiI | CAGNNNCTG | 2 cut(s) | 59, 98 |
| CfoI | GCGC | 1 cut(s) | 141 |
| CviJI | RGCY | 8 cut(s) | 56, 64, 81, 98, 170, 202, 286, 345 |
| CviKI_1 | RGCY | 8 cut(s) | 56, 64, 81, 98, 170, 202, 286, 345 |
| DdeI | CTNAG | 5 cut(s) | 33, 60, 124, 203, 291 |
| DpnI | GATC | 2 cut(s) | 52, 306 |
| DpnII | GATC | 2 cut(s) | 50, 304 |
| Eco130I | CCWWGG | 1 cut(s) | 297 |
| EcoT14I | CCWWGG | 1 cut(s) | 297 |
| ErhI | CCWWGG | 1 cut(s) | 297 |
| FaiI | YATR | 5 cut(s) | 146, 159, 174, 270, 355 |
| FblI | GTMKAC | 1 cut(s) | 145 |
| Fnu4HI | GCNGC | 1 cut(s) | 79 |
| Fsp4HI | GCNGC | 1 cut(s) | 79 |
| GlaI | GCGC | 1 cut(s) | 140 |
| GluI | GCNGC | 1 cut(s) | 79 |
| HhaI | GCGC | 1 cut(s) | 141 |
| Hin6I | GCGC | 1 cut(s) | 139 |
| HinP1I | GCGC | 1 cut(s) | 139 |
| HinfI | GANTC | 1 cut(s) | 293 |
| HphI | GGTGA | 1 cut(s) | 126 |
| Hpy166II | GTNNAC | 2 cut(s) | 146, 189 |
| Hpy188I | TCNGA | 1 cut(s) | 85 |
| Hpy188III | TCNNGA | 2 cut(s) | 59, 93 |
| Hpy8I | GTNNAC | 2 cut(s) | 146, 189 |
| Hpy99I | CGWCG | 1 cut(s) | 344 |
| HpyAV | CCTTC | 1 cut(s) | 242 |
| HpyF3I | CTNAG | 5 cut(s) | 33, 60, 124, 203, 291 |
| HspAI | GCGC | 1 cut(s) | 139 |
| Kzo9I | GATC | 2 cut(s) | 50, 304 |
| LmnI | GCTCC | 2 cut(s) | 61, 95 |
| LpnPI | CCDG | 8 cut(s) | 25, 72, 78, 133, 162, 300, 321, 335 |
| Lsp1109I | GCAGC | 1 cut(s) | 90 |
| MalI | GATC | 2 cut(s) | 52, 306 |
| MboI | GATC | 2 cut(s) | 50, 304 |
| MboII | GAAGA | 2 cut(s) | 108, 173 |
| MluCI | AATT | 4 cut(s) | 130, 152, 175, 279 |
| MlyI | GAGTC | 1 cut(s) | 302 |
| MnlI | CCTC | 1 cut(s) | 198 |
| MroXI | GAANNNNTTC | 1 cut(s) | 8 |
| MseI | TTAA | 6 cut(s) | 21, 111, 179, 237, 282, 324 |
| MspCI | CTTAAG | 1 cut(s) | 236 |
| MvnI | CGCG | 2 cut(s) | 139, 141 |
| NdeII | GATC | 2 cut(s) | 50, 304 |
| PdmI | GAANNNNTTC | 1 cut(s) | 8 |
| PkrI | GCNGC | 1 cut(s) | 80 |
| PleI | GAGTC | 1 cut(s) | 301 |
| PpsI | GAGTC | 1 cut(s) | 301 |
| PstNI | CAGNNNCTG | 2 cut(s) | 59, 98 |
| SaqAI | TTAA | 6 cut(s) | 21, 111, 179, 237, 282, 324 |
| SatI | GCNGC | 1 cut(s) | 79 |
| Sau3AI | GATC | 2 cut(s) | 50, 304 |
| SchI | GAGTC | 1 cut(s) | 302 |
| SetI | ASST | 8 cut(s) | 58, 66, 83, 100, 204, 233, 253, 354 |
| SmlI | CTYRAG | 1 cut(s) | 236 |
| SmoI | CTYRAG | 1 cut(s) | 236 |
| Sse9I | AATT | 4 cut(s) | 130, 152, 175, 279 |
| SsiI | CCGC | 2 cut(s) | 137, 141 |
| StyI | CCWWGG | 1 cut(s) | 297 |
| TasI | AATT | 4 cut(s) | 130, 152, 175, 279 |
| Tru1I | TTAA | 6 cut(s) | 21, 111, 179, 237, 282, 324 |
| Tru9I | TTAA | 6 cut(s) | 21, 111, 179, 237, 282, 324 |
| TseI | GCWGC | 1 cut(s) | 78 |
| TspDTI | ATGAA | 2 cut(s) | 17, 174 |
| Vha464I | CTTAAG | 1 cut(s) | 236 |
| XapI | RAATTY | 1 cut(s) | 130 |
| XmiI | GTMKAC | 1 cut(s) | 145 |
| XmnI | GAANNNNTTC | 1 cut(s) | 8 |