MD06G1044400.v1.1
ERF Family

Belongs to the protein kinase superfamily

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr06
OG0000416
Physical Location & Seq
Reverse (-)
5997264 .. 6000119
2856 bp
Loading structure...
UTR
Exon/CDS
Intron
MD06G1044400.v1.1.491

Sequence Viewer

Length: 1080 bp
ATGAAGTGTTTCTTTCCCTTCAAGGAAAAACGCAAGAGCAAAAAAGAAGTATCGCAATCCGCTCCAGAATTGAGAAACAAAAGCAATCCAAGCAATCCGGCTTTGGATCGCGCGACAAAATCTTCGAGTTCATTGCCATCTCCAAAGAGCATACCAGAATTGTACAAAGAAAGGGAGCATAATTTAAGAGTTTTCTCCTTCCAAGAGCTGAGGGAAGCAACAAATGGTTTCAGCAGGTCGCTTAAGCTCGGGGAAGGAGGTTTTGGGAGGGTTTATAAAGCAGCAATCAGGCCTTCCGATGGAGGAACGGGCAGTCCGATTTGGGTTGCCATCAAGAAGTTAAACCCTCATAGTTTACAGGGTCATAAGCAATGGCTTGCAGAAGTTCAATTTCTTGGCGTCGTAAATCACCCGAATTTGGTAAAACTTCTAGGGGCTTTGCCTGCATTGCCTTGGATGACAAGATTAAAGATAATGCTTGGTGCTGCTCAAGGATTGGCTTATCTACACAAGGGACTCGAAGTCCAGGTAATATATCGAGATTTCAAATCTTCAAATGTGCTATTGGATGAGGAATTCAGGCCAAAGCTCTCAGACTTCGGGCTTGCTAGAGAAGGACCAACGGGTGACCGTTCTCATGTATCAACCTCGGTGGTGGGGACTTATGGATACGCAGCCCCAGAATATTTAAAAACAGGCCATCTTTCAATCAGTTGTGACTTATGGAGTTTTGGTGTGGTACTGTACGAGATCCTAACAGGCAGGCGTGTCTTAGACAAGAACCGCCCCATATCAGAACAAAAGCTTCTTTACTGGGTTAGACAGTTCCCTGCAGACAGTAAAAGGTTCAGCATGGTAATAGATCCCCTACTAAGAGACCAGTATTCTCTTACTGCAGCTCAGAATGTTGCCAAGTTGGCCGATAGCTGTCTGAACAATAACTCAAAGGACCGGCCAACCATGAGTCAGGTGGTAGAGGTGTTGGAGCAAGCTCTACGAGATTCACAAGGGAGCACCAATTCTTTTAAGAGAAGTTTTGAGGTCTCTACATCTAAATTGGCGAAGCGGAAACCCAAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Pkinase Pkinase_Tyr

Protein Analysis

360

Amino Acids

40.24

Weight (kDa)

9.86

Isoelectric Point (pI)

44.65

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PK_Tyr_Ser-Thr PF07714 82 - 147 1.8e-08 Protein tyrosine and serine/threonine kinase
PK_Tyr_Ser-Thr PF07714 152 - 328 6.9e-24 Protein tyrosine and serine/threonine kinase
Pkinase PF00069 157 - 326 2.8e-31 Protein kinase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000416)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G47070
fragaria_vesca FvH4_5g20770 FvH4_5g20770 FvH4_5g20770 FvH4_5g20770 FvH4_7g08930 FvH4_7g08930 FvH4_7g08930 FvH4_7g08930 FvH4_7g08930
malus_domestica MD02G1222400.v1.1 MD04G1053100.v1.1 MD06G1044400.v1.1 MD07G1093200.v1.1
prunus_persica Prupe.2G118500_v2.0.a1 Prupe.2G118500_v2.0.a1 Prupe.2G118500_v2.0.a1 Prupe.2G118500_v2.0.a1 Prupe.2G118500_v2.0.a1 Prupe.5G052200_v2.0.a1 Prupe.5G052200_v2.0.a1
pyrus_communis pycom02g18890 pycom04g04660 pycom06g03780 pycom07g07640
rosa_chinensis RchiOBHm_Chr0c22g0500461 RchiOBHm_Chr0c22g0500491 RchiOBHm_Chr0c22g0500531 RchiOBHm_Chr0c22g0500541 RchiOBHm_Chr0c22g0500641 RchiOBHm_Chr0c22g0500691 RchiOBHm_Chr1g0344381 RchiOBHm_Chr6g0259661 RchiOBHm_Chr6g0259701 RchiOBHm_Chr7g0205471 RchiOBHm_Chr7g0206561 RchiOBHm_Chr7g0206611 RchiOBHm_Chr7g0206731 RchiOBHm_Chr7g0206741 RchiOBHm_Chr7g0206781 RchiOBHm_Chr7g0206801 RchiOBHm_Chr7g0206811 RchiOBHm_Chr7g0206831 RchiOBHm_Chr7g0206861 RchiOBHm_Chr7g0206891 RchiOBHm_Chr7g0206981 RchiOBHm_Chr7g0206991 RchiOBHm_Chr7g0207071 RchiOBHm_Chr7g0207121 RchiOBHm_Chr7g0207151 RchiOBHm_Chr7g0207181 RchiOBHm_Chr7g0207241 RchiOBHm_Chr7g0207251 RchiOBHm_Chr7g0207261 RchiOBHm_Chr7g0207411 RchiOBHm_Chr7g0207471 RchiOBHm_Chr7g0207701
rosa_laevigata RLG00000003296 RLG00000003302 RLG00000003326 RLG00000003346 RLG00000028901
rosa_multiflora Rmu_sc0000536.1_g000001 Rmu_sc0000536.1_g000002 Rmu_sc0004987.1_g000003 Rmu_sc0008509.1_g000002 Rmu_sc0008509.1_g000029 Rmu_sc0012558.1_g000001 Rmu_sc0013160.1_g000002 Rmu_sc0014912.1_g000004
rosa_roxburghii Rroxscaffold_3G00251310 Rroxscaffold_3G00251320 Rroxscaffold_3G00251410 Rroxscaffold_3G00252190 Rroxscaffold_4G00309810 Rroxscaffold_7G00206970 Rroxscaffold_7G00207090
rosa_rugosa Rorug01G0173500 Rorug01G0173600 Rorug05G0590200 Rorug07G0096400 Rorug07G0096700 Rorug07G0096900 Rorug07G0098500 Rorug07G0099000
rosa_samantha Rh1AG189700 Rh1BG156700 Rh1DG188100 Rh7BG221600 Rh7BG225000 Rh7BG225300 Rh7BG227500 Rh7BG227700 Rh7CG244800
rosa_wichuraiana Rw1G015650 Rw6G009210 Rw7G019820 Rw7G019850

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 276
Acc36I ACCTGC 1 cut(s) 225
AccBSI CCGCTC 1 cut(s) 62
AccII CGCG 2 cut(s) 111, 113
AciI CCGC 3 cut(s) 60, 784, 1066
AclWI GGATC 3 cut(s) 114, 745, 857
AcoI YGGCCR 2 cut(s) 918, 953
AcsI RAATTY 2 cut(s) 415, 575
AcyI GRCGYC 1 cut(s) 399
AfaI GTAC 3 cut(s) 164, 741, 746
AfiI CCNNNNNNNGG 2 cut(s) 299, 418
AflII CTTAAG 1 cut(s) 242
AgsI TTSAA 5 cut(s) 22, 389, 547, 555, 708
AhdI GACNNNNNGTC 1 cut(s) 521
AjnI CCWGG 1 cut(s) 525
AjuI GAANNNNNNNTTGG 2 cut(s) 246, 278
AloI GAACNNNNNNTCC 2 cut(s) 298, 330
AluBI AGCT 7 cut(s) 208, 247, 589, 805, 899, 927, 992
AluI AGCT 7 cut(s) 208, 247, 589, 805, 899, 927, 992
Alw21I GWGCWC 1 cut(s) 1016
Alw26I GTCTC 2 cut(s) 870, 1048
AlwI GGATC 3 cut(s) 114, 745, 857
Ama87I CYCGRG 1 cut(s) 248
AoxI GGCC 5 cut(s) 290, 581, 697, 918, 953
ApeKI GCWGC 4 cut(s) 281, 485, 674, 896
ApoI RAATTY 2 cut(s) 415, 575
ArsI GACNNNNNNTTYG 2 cut(s) 106, 138
Asp700I GAANNNNTTC 1 cut(s) 8
AspLEI GCGC 1 cut(s) 113
AspS9I GGNCC 2 cut(s) 617, 949
AsuHPI GGTGA 2 cut(s) 401, 638
AvaI CYCGRG 1 cut(s) 248
AvaII GGWCC 2 cut(s) 617, 949
Bbv12I GWGCWC 1 cut(s) 1016
BbvCI CCTCAGC 1 cut(s) 209
BbvI GCAGC 4 cut(s) 293, 472, 686, 908
BccI CCATC 4 cut(s) 145, 293, 338, 708
BcgI CGANNNNNNTGC 2 cut(s) 115, 149
BciT130I CCWGG 1 cut(s) 527
BciVI GTATCC 1 cut(s) 662
BcoDI GTCTC 2 cut(s) 870, 1048
BfaI CTAG 2 cut(s) 431, 609
BfmI CTRYAG 2 cut(s) 831, 894
BfrI CTTAAG 1 cut(s) 242
BfuAI ACCTGC 1 cut(s) 225
BfuI GTATCC 1 cut(s) 662
BglI GCCNNNNNGGC 1 cut(s) 917
BisI GCNGC 4 cut(s) 282, 486, 675, 897
BlsI GCNGC 4 cut(s) 283, 487, 676, 898
Bme1390I CCNGG 1 cut(s) 527
Bme18I GGWCC 2 cut(s) 617, 949
BmeRI GACNNNNNGTC 1 cut(s) 521
BmeT110I CYCGRG 1 cut(s) 248
BmgT120I GGNCC 2 cut(s) 617, 949
BmrFI CCNGG 1 cut(s) 527
BmrI ACTGGG 1 cut(s) 823
BmuI ACTGGG 1 cut(s) 823
BpmI CTGGAG 1 cut(s) 48
Bpu10I CCTNAGC 1 cut(s) 209
BpuEI CTTGAG 1 cut(s) 474
BsaHI GRCGYC 1 cut(s) 399
BsaI GGTCTC 2 cut(s) 870, 1048
BsaJI CCNNGG 2 cut(s) 452, 648
BsaXI ACNNNNNCTCC 2 cut(s) 718, 748
Bsc4I CCNNNNNNNGG 2 cut(s) 299, 418
Bse118I RCCGGY 1 cut(s) 951
Bse1I ACTGG 2 cut(s) 818, 880
Bse3DI GCAATG 3 cut(s) 131, 377, 446
BseBI CCWGG 1 cut(s) 527
BseDI CCNNGG 2 cut(s) 452, 648
BseGI GGATG 2 cut(s) 462, 574
BseLI CCNNNNNNNGG 2 cut(s) 299, 418
BseMI GCAATG 3 cut(s) 131, 377, 446
BseMII CTCAG 3 cut(s) 200, 606, 914
BseNI ACTGG 2 cut(s) 818, 880
BseXI GCAGC 4 cut(s) 293, 472, 686, 908
Bsh1236I CGCG 2 cut(s) 111, 113
BshFI GGCC 5 cut(s) 292, 583, 699, 920, 955
BsiHKAI GWGCWC 1 cut(s) 1016
BsiHKCI CYCGRG 1 cut(s) 248
BsiSI CCGG 2 cut(s) 98, 952
BslFI GGGAC 2 cut(s) 528, 673
BslI CCNNNNNNNGG 2 cut(s) 299, 418
BsmAI GTCTC 2 cut(s) 870, 1048
BsmFI GGGAC 2 cut(s) 528, 673
BsnI GGCC 5 cut(s) 292, 583, 699, 920, 955
Bso31I GGTCTC 2 cut(s) 870, 1048
BsoBI CYCGRG 1 cut(s) 248
Bsp1286I GDGCHC 1 cut(s) 1016
Bsp1407I TGTACA 1 cut(s) 162
Bsp143I GATC 3 cut(s) 106, 750, 862
BspACI CCGC 3 cut(s) 60, 784, 1066
BspANI GGCC 5 cut(s) 292, 583, 699, 920, 955
BspCNI CTCAG 3 cut(s) 201, 605, 913
BspFNI CGCG 2 cut(s) 111, 113
BspMAI CTGCAG 2 cut(s) 835, 898
BspMI ACCTGC 1 cut(s) 225
BspPI GGATC 3 cut(s) 114, 745, 857
BspTI CTTAAG 1 cut(s) 242
BspTNI GGTCTC 2 cut(s) 870, 1048
BsrBI CCGCTC 1 cut(s) 62
BsrDI GCAATG 3 cut(s) 131, 377, 446
BsrFI RCCGGY 1 cut(s) 951
BsrGI TGTACA 1 cut(s) 162
BsrI ACTGG 2 cut(s) 818, 880
BssAI RCCGGY 1 cut(s) 951
BssECI CCNNGG 2 cut(s) 452, 648
BssMI GATC 3 cut(s) 106, 750, 862
BssNI GRCGYC 1 cut(s) 399
BssT1I CCWWGG 1 cut(s) 452
Bst2UI CCWGG 1 cut(s) 527
Bst4CI ACNGT 4 cut(s) 632, 744, 825, 839
BstACI GRCGYC 1 cut(s) 399
BstAFI CTTAAG 1 cut(s) 242
BstAUI TGTACA 1 cut(s) 162
BstC8I GCNNGC 5 cut(s) 378, 444, 606, 764, 990
BstDEI CTNAG 5 cut(s) 209, 592, 772, 872, 900
BstEII GGTNACC 1 cut(s) 626
BstF5I GGATG 2 cut(s) 462, 574
BstFNI CGCG 2 cut(s) 111, 113
BstHHI GCGC 1 cut(s) 113
BstKTI GATC 3 cut(s) 109, 753, 865
BstMAI GTCTC 2 cut(s) 870, 1048
BstMBI GATC 3 cut(s) 106, 750, 862
BstMWI GCNNNNNNNGC 4 cut(s) 90, 443, 448, 917
BstNI CCWGG 1 cut(s) 527
BstPI GGTNACC 1 cut(s) 626
BstSCI CCNGG 1 cut(s) 525
BstSFI CTRYAG 2 cut(s) 831, 894
BstUI CGCG 2 cut(s) 111, 113
BstV1I GCAGC 4 cut(s) 293, 472, 686, 908
BstX2I RGATCY 2 cut(s) 750, 862
BstYI RGATCY 2 cut(s) 750, 862
BsuI GTATCC 1 cut(s) 662
BsuRI GGCC 5 cut(s) 292, 583, 699, 920, 955
BtsCI GGATG 2 cut(s) 462, 574
BveI ACCTGC 1 cut(s) 225
Cac8I GCNNGC 5 cut(s) 378, 444, 606, 764, 990
CfoI GCGC 1 cut(s) 113
Cfr10I RCCGGY 1 cut(s) 951
Cfr13I GGNCC 2 cut(s) 617, 949
CseI GACGC 1 cut(s) 388
Csp6I GTAC 3 cut(s) 163, 740, 745
CspCI CAANNNNNGTGG 2 cut(s) 633, 668
CviAII CATG 3 cut(s) 638, 853, 961
CviQI GTAC 3 cut(s) 163, 740, 745
DdeI CTNAG 5 cut(s) 209, 592, 772, 872, 900
DpnI GATC 3 cut(s) 108, 752, 864
DpnII GATC 3 cut(s) 106, 750, 862
DraI TTTAAA 1 cut(s) 690
DriI GACNNNNNGTC 1 cut(s) 521
EaeI YGGCCR 2 cut(s) 918, 953
Eam1105I GACNNNNNGTC 1 cut(s) 521
Eco130I CCWWGG 1 cut(s) 452
Eco147I AGGCCT 1 cut(s) 292
Eco31I GGTCTC 2 cut(s) 870, 1048
Eco47I GGWCC 2 cut(s) 617, 949
Eco88I CYCGRG 1 cut(s) 248
Eco91I GGTNACC 1 cut(s) 626
EcoO65I GGTNACC 1 cut(s) 626
EcoRI GAATTC 1 cut(s) 575
EcoRII CCWGG 1 cut(s) 525
EcoT14I CCWWGG 1 cut(s) 452
ErhI CCWWGG 1 cut(s) 452
FaeI CATG 3 cut(s) 641, 856, 964
FalI AAGNNNNNCTT 1 cut(s) 28
FaqI GGGAC 2 cut(s) 528, 673
FatI CATG 3 cut(s) 637, 852, 960
Fnu4HI GCNGC 4 cut(s) 282, 486, 675, 897
FokI GGATG 2 cut(s) 469, 581
Fsp4HI GCNGC 4 cut(s) 282, 486, 675, 897
FspBI CTAG 2 cut(s) 431, 609
GlaI GCGC 1 cut(s) 112
GluI GCNGC 4 cut(s) 282, 486, 675, 897
GsuI CTGGAG 1 cut(s) 48
HaeIII GGCC 5 cut(s) 292, 583, 699, 920, 955
HapII CCGG 2 cut(s) 98, 952
HgaI GACGC 1 cut(s) 388
HhaI GCGC 1 cut(s) 113
Hin1I GRCGYC 1 cut(s) 399
Hin1II CATG 3 cut(s) 641, 856, 964
Hin6I GCGC 1 cut(s) 111
HinP1I GCGC 1 cut(s) 111
HindIII AAGCTT 1 cut(s) 803
HinfI GANTC 3 cut(s) 516, 964, 1001
HpaII CCGG 2 cut(s) 98, 952
HphI GGTGA 2 cut(s) 401, 638
Hpy166II GTNNAC 1 cut(s) 356
Hpy188I TCNGA 6 cut(s) 298, 318, 595, 796, 903, 933
Hpy188III TCNNGA 3 cut(s) 65, 334, 539
Hpy8I GTNNAC 1 cut(s) 356
Hpy99I CGWCG 1 cut(s) 404
HpyAV CCTTC 5 cut(s) 28, 208, 248, 303, 608
HpyCH4III ACNGT 4 cut(s) 632, 744, 825, 839
HpyCH4V TGCA 4 cut(s) 380, 446, 833, 896
HpyF10VI GCNNNNNNNGC 4 cut(s) 90, 443, 448, 917
HpyF3I CTNAG 5 cut(s) 209, 592, 772, 872, 900
Hsp92I GRCGYC 1 cut(s) 399
Hsp92II CATG 3 cut(s) 641, 856, 964
HspAI GCGC 1 cut(s) 111
Kzo9I GATC 3 cut(s) 106, 750, 862
LmnI GCTCC 4 cut(s) 67, 175, 985, 1011
Lsp1109I GCAGC 4 cut(s) 293, 472, 686, 908
MaeI CTAG 2 cut(s) 431, 609
MaeIII GTNAC 2 cut(s) 626, 716
MalI GATC 3 cut(s) 108, 752, 864
MbiI CCGCTC 1 cut(s) 62
MboI GATC 3 cut(s) 106, 750, 862
MboII GAAGA 2 cut(s) 114, 543
MflI RGATCY 2 cut(s) 750, 862
MhlI GDGCHC 1 cut(s) 1016
MluCI AATT 8 cut(s) 68, 158, 181, 389, 415, 575, 1018, 1055
MlyI GAGTC 2 cut(s) 510, 973
MmeI TCCRAC 1 cut(s) 963
MnlI CCTC 9 cut(s) 204, 251, 261, 296, 357, 565, 658, 970, 1033
MroXI GAANNNNTTC 1 cut(s) 8
MseI TTAA 6 cut(s) 185, 243, 341, 467, 689, 1026
MspCI CTTAAG 1 cut(s) 242
MspI CCGG 2 cut(s) 98, 952
MspR9I CCNGG 1 cut(s) 527
MvaI CCWGG 1 cut(s) 527
MvnI CGCG 2 cut(s) 111, 113
MwoI GCNNNNNNNGC 4 cut(s) 90, 443, 448, 917
NdeII GATC 3 cut(s) 106, 750, 862
NlaIII CATG 3 cut(s) 641, 856, 964
NmuCI GTSAC 2 cut(s) 626, 716
PceI AGGCCT 1 cut(s) 292
PcsI WCGNNNNNNNCGW 1 cut(s) 314
PdmI GAANNNNTTC 1 cut(s) 8
PfeI GAWTC 1 cut(s) 1001
PkrI GCNGC 4 cut(s) 283, 487, 676, 898
PleI GAGTC 2 cut(s) 510, 972
PpsI GAGTC 2 cut(s) 510, 972
PsiI TTATAA 1 cut(s) 276
Psp6I CCWGG 1 cut(s) 525
PspEI GGTNACC 1 cut(s) 626
PspGI CCWGG 1 cut(s) 525
PspPI GGNCC 2 cut(s) 617, 949
PstI CTGCAG 2 cut(s) 835, 898
PsuI RGATCY 2 cut(s) 750, 862
RsaI GTAC 3 cut(s) 164, 741, 746
RsaNI GTAC 3 cut(s) 163, 740, 745
SaqAI TTAA 6 cut(s) 185, 243, 341, 467, 689, 1026
SatI GCNGC 4 cut(s) 282, 486, 675, 897
Sau3AI GATC 3 cut(s) 106, 750, 862
Sau96I GGNCC 2 cut(s) 617, 949
SchI GAGTC 2 cut(s) 510, 973
ScrFI CCNGG 1 cut(s) 527
SduI GDGCHC 1 cut(s) 1016
SfcI CTRYAG 2 cut(s) 831, 894
SinI GGWCC 2 cut(s) 617, 949
SmlI CTYRAG 2 cut(s) 242, 489
SmoI CTYRAG 2 cut(s) 242, 489
Sse9I AATT 8 cut(s) 68, 158, 181, 389, 415, 575, 1018, 1055
SseBI AGGCCT 1 cut(s) 292
SsiI CCGC 3 cut(s) 60, 784, 1066
SspI AATATT 1 cut(s) 686
SspMI CTAG 2 cut(s) 431, 609
StuI AGGCCT 1 cut(s) 292
StyD4I CCNGG 1 cut(s) 525
StyI CCWWGG 1 cut(s) 452
TaaI ACNGT 4 cut(s) 632, 744, 825, 839
TaqI TCGA 3 cut(s) 125, 519, 538
TasI AATT 8 cut(s) 68, 158, 181, 389, 415, 575, 1018, 1055
TatI WGTACW 1 cut(s) 162
TfiI GAWTC 1 cut(s) 1001
Tru1I TTAA 6 cut(s) 185, 243, 341, 467, 689, 1026
Tru9I TTAA 6 cut(s) 185, 243, 341, 467, 689, 1026
TseFI GTSAC 2 cut(s) 626, 716
TseI GCWGC 4 cut(s) 281, 485, 674, 896
Tsp45I GTSAC 2 cut(s) 626, 716
TspDTI ATGAA 2 cut(s) 17, 120
Vha464I CTTAAG 1 cut(s) 242
VpaK11BI GGWCC 2 cut(s) 617, 949
XapI RAATTY 2 cut(s) 415, 575
XcmI CCANNNNNNNNNTGG 1 cut(s) 967
XmnI GAANNNNTTC 1 cut(s) 8
XspI CTAG 2 cut(s) 431, 609
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.