Belongs to the protein kinase superfamily
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AfiI | CCNNNNNNNGG | 1 cut(s) | 157 |
| AgsI | TTSAA | 2 cut(s) | 128, 206 |
| AjnI | CCWGG | 1 cut(s) | 169 |
| AluBI | AGCT | 2 cut(s) | 166, 242 |
| AluI | AGCT | 2 cut(s) | 166, 242 |
| AsuHPI | GGTGA | 1 cut(s) | 140 |
| BceAI | ACGGC | 1 cut(s) | 224 |
| BciT130I | CCWGG | 1 cut(s) | 171 |
| BfmI | CTRYAG | 1 cut(s) | 234 |
| Bme1390I | CCNGG | 1 cut(s) | 171 |
| BmrFI | CCNGG | 1 cut(s) | 171 |
| Bsc4I | CCNNNNNNNGG | 1 cut(s) | 157 |
| Bse1I | ACTGG | 1 cut(s) | 219 |
| BseBI | CCWGG | 1 cut(s) | 171 |
| BseLI | CCNNNNNNNGG | 1 cut(s) | 157 |
| BseNI | ACTGG | 1 cut(s) | 219 |
| BslI | CCNNNNNNNGG | 1 cut(s) | 157 |
| BsrI | ACTGG | 1 cut(s) | 219 |
| Bst2UI | CCWGG | 1 cut(s) | 171 |
| BstAPI | GCANNNNNTGC | 1 cut(s) | 116 |
| BstC8I | GCNNGC | 1 cut(s) | 117 |
| BstDEI | CTNAG | 1 cut(s) | 243 |
| BstMWI | GCNNNNNNNGC | 1 cut(s) | 116 |
| BstNI | CCWGG | 1 cut(s) | 171 |
| BstSCI | CCNGG | 1 cut(s) | 169 |
| BstSFI | CTRYAG | 1 cut(s) | 234 |
| Cac8I | GCNNGC | 1 cut(s) | 117 |
| CviAII | CATG | 2 cut(s) | 35, 111 |
| CviJI | RGCY | 4 cut(s) | 115, 166, 211, 242 |
| CviKI_1 | RGCY | 4 cut(s) | 115, 166, 211, 242 |
| DdeI | CTNAG | 1 cut(s) | 243 |
| EcoRII | CCWGG | 1 cut(s) | 169 |
| EcoT22I | ATGCAT | 1 cut(s) | 6 |
| FaeI | CATG | 2 cut(s) | 38, 114 |
| FalI | AAGNNNNNCTT | 2 cut(s) | 99, 131 |
| FatI | CATG | 2 cut(s) | 34, 110 |
| Hin1II | CATG | 2 cut(s) | 38, 114 |
| HindIII | AAGCTT | 2 cut(s) | 164, 240 |
| HinfI | GANTC | 1 cut(s) | 202 |
| HphI | GGTGA | 1 cut(s) | 140 |
| HpyCH4V | TGCA | 3 cut(s) | 4, 55, 119 |
| HpyF10VI | GCNNNNNNNGC | 1 cut(s) | 116 |
| HpyF3I | CTNAG | 1 cut(s) | 243 |
| Hsp92II | CATG | 2 cut(s) | 38, 114 |
| LpnPI | CCDG | 5 cut(s) | 72, 143, 156, 183, 200 |
| MboII | GAAGA | 1 cut(s) | 56 |
| MluCI | AATT | 2 cut(s) | 71, 128 |
| MnlI | CCTC | 1 cut(s) | 115 |
| Mph1103I | ATGCAT | 1 cut(s) | 6 |
| MslI | CAYNNNNRTG | 1 cut(s) | 39 |
| MspR9I | CCNGG | 1 cut(s) | 171 |
| MvaI | CCWGG | 1 cut(s) | 171 |
| MwoI | GCNNNNNNNGC | 1 cut(s) | 116 |
| NlaIII | CATG | 2 cut(s) | 38, 114 |
| NsiI | ATGCAT | 1 cut(s) | 6 |
| PfeI | GAWTC | 1 cut(s) | 202 |
| PfoI | TCCNGGA | 1 cut(s) | 169 |
| Psp6I | CCWGG | 1 cut(s) | 169 |
| PspGI | CCWGG | 1 cut(s) | 169 |
| RseI | CAYNNNNRTG | 1 cut(s) | 39 |
| ScrFI | CCNGG | 1 cut(s) | 171 |
| SetI | ASST | 3 cut(s) | 126, 168, 244 |
| SfcI | CTRYAG | 1 cut(s) | 234 |
| SgeI | CNNG | 9 cut(s) | 47, 71, 123, 128, 146, 170, 182, 183, 227 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 39 |
| Sse9I | AATT | 2 cut(s) | 71, 128 |
| StyD4I | CCNGG | 1 cut(s) | 169 |
| TaqI | TCGA | 1 cut(s) | 77 |
| TasI | AATT | 2 cut(s) | 71, 128 |
| TfiI | GAWTC | 1 cut(s) | 202 |
| TspDTI | ATGAA | 2 cut(s) | 57, 237 |
| Zsp2I | ATGCAT | 1 cut(s) | 6 |