MD04G1110500.v1.1

LRR receptor-like serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr04
OG0000262
Physical Location & Seq
Reverse (-)
19508936 .. 19512503
3568 bp
Loading structure...
UTR
Exon/CDS
Intron
MD04G1110500.v1.1.491

Sequence Viewer

Length: 3324 bp
ATGCATTCCTGTAAGCGAAACTTGGTTACTAATTTCACAGCCAAAAGAGAGAGAAAAACAGAGAGTTTCTTATTTGCAGTCCGTATTTTGGTAAGGATGGAGCATTCACGTCGAATTCATGGCAGGCTGATTTTGTTTAAACTCTTTCATGGCTTCATTCTTTTATGCATGACCACACGTCTGGGATTTGCAGCACTGTCCAGCACTAGTCTTGGAAATGAAACTGATCGCCTGGCGCTGCTCGACTTCAAGAAACACATCACTCAAGATGCTCTCCATGTCATGAGCGCATGGAATGATTCCATCCATTTCTGCAGTTGGGCTGGCGTTACATGCAGCCATTCCACCAAAAGAGTCTTGATTTTGAACCTGCCAGATCAAAACTTGGCAGGCTCTTTACCTCCTTCTATAGGAAATCTTTCCTATCTTACTAGTATCAACCTAGGAAGCAACAGCTTTCGTGGTGAAATTCCTCAGGAAATTGGTCGTCTGCAAAGCCTACACGTTCTCAACTTGTCTCACAATTCCTTCAGCGGTAAAATTCCAACCAATTTATCCCAGTGTACCCAACTAAAGACGCTTGAGTTTCGTTACAATGAACTTATTGGCTCGATTCCAAAACAACTTAGTTCATTGTTGAAATTAAGGCGTCTGTTGCTTTCTACTAACAATCTGACTGGAACCATCCCAAGTTGGATGGGAAACTTTTCTTCTCTGCATGTGTTTGATCTTAGCGGAAACAATTTTCAAGGAAGCATACCCAATGATCTCGGGCGGCTAACAAGATTGCAATTTGTCGGACTTCAAGCAATCAATCTCTCTGGTATAATCCCTGCTTCAATCTATAATATTTCTTCCATACACACGTTTGGTGTTGGTTTCAACCAACTGCATGGAGAGCTACCACCAAATGTCGGCCTTACTCTTCCTAATCTCCAAGAATTTGCATGTGGCGGGAACAAATTCACAGGAAATATTCCTATATCATTGTCAAATGCTTCTGGACTACAACTTATTGACTTCGCTGAAAATGGTCTTACTGGAACAATCCCAGGTGAAAATCTTGGAACCTTGCGAAGCTTAGTTGTTCTAAACCTTTATCACAATAGACTGGGAAGTGGGAAAGCTGGCGACCTGAATTTTCTCAAATTCTTGGCTAATTGTACTAGTCTGGAGGTTTTGGATATTGTTGATAATCAATTTGGAGGAGAATTGCCCGTATCCATGTCCAATCTTTCTACCACACTAAGAAGTCTTTATCTGGGGACAAATTCCATACATGGAAGCGTCCCTTTAGGTATTGGAAATCTTCTAAACTTGGCCGCTATTGGAATGGAAAATAACTATTTCAGTGGTAGTCTCCCTGAAGAAATTGGGAGGCTTAAGAAGTTAGAGGGGTTGAGTTTGGGCGGCAACAATTTTTCGGGGCCGATCCCGTCGTCCCTAGGTAACATGACTTCGCTGACACAGCTCTACATGGATGACAATGGATTTGAGGGAAGAATACATCCGAGTCTAGGAAACTGCCAACGTCTACTATTCCTTGACCTTTCTAGTAACAATCTAACAGGCACCATACCTAAAGAGCTTTTCCGGATTTCTTCCATCTCAATTTACATGCCTAACAATCAGTTGACCGGTCCACTACCATCCGAAGTTGGAAATTTGGTAAATCTAGTGGAGCTATATGTATCAGGAAACAAGTTATCAGGTGAAATCCCCACAAGCCTTGGCAGTTGTATTATGTTGGTGCTCTTGCATTTGAAAGGTAATGAGTTTCAAGGAATAATTCCTATATCTCTCAAAAGTTTAAGAAGCCTGGAAGAAATTGATCTTTCGAGTAATAACTTATCTGGGCAGATTCCTGAATTTCTAGGCAAATTTAGATTTCTTAGTCATCTCAACCTTTCATATAACAATTTTGAGGGTAAAATGCCTGAAGAAGGAATCTTTTCAAATGCAACTAGTCTCTCAATTCTTGGAAATCCTAAGCTCTGTGGTGGCAGCTCGAAATTACATTTACCTGCATGCCCCAACACAAAGCCTCCCTCATCTCGAGGATTGCTTGCTCGAAAAGTAGTCATCTCTGTAGCTTGTGCGCTTGCCTTCATAATTGGTATGTCATGCTTTCATGTTGCTCGTTCAATGCTTAAGAGGCCAAGGAGTATACCAGTAAGCTCACGTTCTTATAAAGATTGGAAATCAGGTGTCTCTTACTCAGAACTTGTTCAATCTACTGACGGGTTCTCTAGAGAAAATCTGATTGGTTCTGGAAGTTTTGGTTCTGTTTACAAAGGAGTAGTCCCCGGTGATGGAACACTAGTTGCAGTTAAGGTATTAAACCTTCAACAACAAGGAGCATCCAAGAGTTTTATGGATGAATGCAAAGCTTTGAGAAGCATAAGGCACCGTAATCTTCTGAAGATCATAACTGCATGCTCAAGCATTGACAACCAGGGTCAAGATTTCAAAAGTCTAGTTTTTGAGTACATAGTAAATGGAAGCTTAGAAATGTGGCTGCATCTGAAAAATGATGAGCACACTCACAGTAAGAGATTGAGCCTTGTCCAAAGACTCAATATTGCAATTGATGTTGCTTCCGCATTGGATTATATCCACCACCATTGTGAAACTTCAATTGTTCATTGTGATATGAAGCCAAATAATGTTCTTCTTGACGAAGATATGGTAGCTCATGTTGGTGACTTTGGTTTAGCAAGGTTCCTCTTGGAGGCCTCTAATGATCTTGCTCAAAGTCAAACCATATCAACCGGGTTAAAGGGTTCAATAGGCTACATTCCTCCAGAGTATGGCATGGGAGGCCAAGTTTCCATCCTTGGAGATGTTTATAGTTTTGGAATACTATTGCTAGAAATGTTCACAGGAAGACGACCTACCGATGACATGTTTAAAGATGGTTTGAGCATTCACCAATTCACTGCCATGGCGATGCCTGACCATGCCATGGACATTGTTGACCCATTGCTGCTCGTCGAATGTGAAGATGCAAATGTTGATGGAAGCCCAATTCAAGCAAGAAGGTTGGTGGAATGCTCAGTTTCAGTGATGCAAATTGGACTCTCATGCTCTGCCACATCACCATCAAAGCGAATGCTTATGGATGCTGTTGTGAACAAATTAAAGACAATTAGAGATTCATATCTCAATTTAAGAGGTAAAGGTAAGAAATATAAATGTGAGAGTTCCATTCTCCTAGAACCTGTGGTCCAAGAAAATTATGGTCATTCACCGTTCAATCTTCATCCTACAGTGGAGGACATAAAATCGAATGGTGAGAGACCACACTTGAACCTTGTGGTCTAG

Protein Analysis

1108

Amino Acids

120.99

Weight (kDa)

8.33

Isoelectric Point (pI)

38.97

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRRNT_2 PF08263 74 - 113 9.4e-11 Leucine rich repeat N-terminal domain
LRR_14 PF23598 119 - 219 2e-07 Leucine-rich repeat region
LRR_14 PF23598 233 - 424 1.8e-07 Leucine-rich repeat region
LRR_14 PF23598 415 - 520 2.3e-07 Leucine-rich repeat region
LRR_14 PF23598 504 - 657 3.2e-07 Leucine-rich repeat region
LRR_8 PF13855 583 - 641 3.8e-06 Leucine rich repeat
Pkinase PF00069 750 - 978 6.1e-38 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 752 - 990 1.6e-38 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000262)

Species Orthologous Gene IDs
fragaria_vesca FvH4_6g11680 FvH4_6g11680 FvH4_6g11680 FvH4_6g11680 FvH4_6g11680 FvH4_6g11680 FvH4_6g11681 FvH4_6g11682 FvH4_6g11683
malus_domestica MD00G1079100.v1.1 MD00G1080000.v1.1 MD01G1100900.v1.1 MD03G1225200.v1.1 MD04G1041600.v1.1 MD04G1110500.v1.1 MD04G1118700.v1.1 MD04G1150300.v1.1 MD04G1150400.v1.1 MD04G1156000.v1.1 MD04G1156200.v1.1 MD04G1156700.v1.1 MD04G1157400.v1.1 MD04G1157500.v1.1 MD04G1157700.v1.1 MD04G1158000.v1.1 MD04G1158200.v1.1 MD04G1158600.v1.1 MD04G1159000.v1.1 MD04G1159100.v1.1 MD05G1061600.v1.1 MD05G1075800.v1.1 MD06G1129300.v1.1 MD07G1217800.v1.1 MD07G1218000.v1.1 MD07G1308700.v1.1 MD12G1130700.v1.1 MD12G1130800.v1.1 MD12G1134500.v1.1 MD12G1134700.v1.1 MD12G1135000.v1.1 MD12G1135200.v1.1 MD12G1135800.v1.1 MD12G1164300.v1.1 MD12G1164400.v1.1 MD12G1169100.v1.1 MD12G1169700.v1.1 MD12G1170200.v1.1 MD12G1170400.v1.1 MD13G1275300.v1.1
prunus_persica Prupe.1G072900_v2.0.a1 Prupe.1G194200_v2.0.a1 Prupe.1G201100_v2.0.a1 Prupe.6G248000_v2.0.a1 Prupe.6G248100_v2.0.a1 Prupe.6G248500_v2.0.a1 Prupe.6G274800_v2.0.a1 Prupe.6G274900_v2.0.a1 Prupe.6G275100_v2.0.a1 Prupe.6G275100_v2.0.a1 Prupe.7G044200_v2.0.a1 Prupe.7G044300_v2.0.a1 Prupe.7G044700_v2.0.a1
pyrus_communis pycom04g03530 pycom04g10490 pycom04g10500 pycom04g10510 pycom04g10560 pycom04g13580 pycom04g13600 pycom04g14080 pycom12g10580 pycom12g11020 pycom12g12650 pycom12g12680 pycom12g15730 pycom12g15740 pycom12g16060 pycom12g16100 pycom12g16200 pycom12g16240 pycom16g21060
rosa_chinensis RchiOBHm_Chr3g0463401 RchiOBHm_Chr3g0463411 RchiOBHm_Chr3g0463481 RchiOBHm_Chr4g0408571 RchiOBHm_Chr4g0408641
rosa_laevigata RLG00000019546 RLG00000019547 RLG00000019552 RLG00000019553 RLG00000019554 RLG00000019578 RLG00000024784 RLG00000024785 RLG00000024786
rosa_multiflora Rmu_sc0000923.1_g000013 Rmu_sc0000923.1_g000017 Rmu_sc0000923.1_g000018 Rmu_sc0001053.1_g000005 Rmu_sc0001053.1_g000012 Rmu_sc0001053.1_g000013 Rmu_sc0001053.1_g000014 Rmu_sc0031971.1_g000001
rosa_roxburghii Rroxscaffold_2G00107980 Rroxscaffold_6G00416540 Rroxscaffold_6G00416560
rosa_rugosa Rorug02G0333800 Rorug02G0333900 Rorug03G0064500 Rorug03G0064600 Rorug03G0064800 Rorug03G0064900 Rorug03G0065000 Rorug03G0065000
rosa_samantha Rh2CG370900 Rh3AG122100 Rh3BG126500 Rh3BG126800 Rh3BG126900 Rh3CG128600 Rh3CG128700 Rh3DG127400 Rh3DG127500 Rh3DG127700 Rh4CG158500 Rh4CG158600
rosa_wichuraiana Rw0G010610 Rw0G010620 Rw3G010370 Rw3G010380 Rw3G010400 Rw3G010410

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 2190
Acc36I ACCTGC 2 cut(s) 378, 2032
AccB1I GGYRCC 2 cut(s) 1571, 2406
AccB7I CCANNNNNTGG 2 cut(s) 2810, 2965
AccI GTMKAC 2 cut(s) 1534, 2167
AccIII TCCGGA 1 cut(s) 1593
AciI CCGC 7 cut(s) 534, 735, 775, 954, 1323, 1410, 2601
AclWI GGATC 1 cut(s) 1426
AcoI YGGCCR 1 cut(s) 1320
AcuI CTGAAG 4 cut(s) 514, 1386, 1959, 2441
AcyI GRCGYC 1 cut(s) 649
AfaI GTAC 3 cut(s) 565, 1165, 2489
AfiI CCNNNNNNNGG 9 cut(s) 88, 410, 914, 1517, 1943, 2312, 2731, 2810, 2965
AflII CTTAAG 2 cut(s) 1382, 2150
AflIII ACRYGT 4 cut(s) 176, 502, 864, 2904
AgeI ACCGGT 1 cut(s) 1637
AhdI GACNNNNNGTC 1 cut(s) 177
AhlI ACTAGT 5 cut(s) 206, 431, 1166, 1964, 2320
AjiI CACGTC 2 cut(s) 110, 179
AjnI CCWGG 4 cut(s) 231, 1051, 1818, 2454
AjuI GAANNNNNNNTTGG 2 cut(s) 2247, 2279
AleI CACNNNNGTG 1 cut(s) 2625
AloI GAACNNNNNNTCC 2 cut(s) 3210, 3242
Alw21I GWGCWC 2 cut(s) 1755, 2541
Alw26I GTCTC 5 cut(s) 522, 1364, 1973, 2215, 3292
AlwI GGATC 1 cut(s) 1426
Ama87I CYCGRG 2 cut(s) 770, 2055
Aor13HI TCCGGA 1 cut(s) 1593
AoxI GGCC 6 cut(s) 916, 1320, 1427, 2156, 2733, 2821
ApeKI GCWGC 6 cut(s) 191, 238, 336, 2004, 2518, 2986
ArsI GACNNNNNNTTYG 4 cut(s) 1475, 1507, 2286, 2318
AsiGI ACCGGT 1 cut(s) 1637
Asp700I GAANNNNTTC 5 cut(s) 418, 706, 962, 1951, 2226
AspA2I CCTAGG 2 cut(s) 442, 1444
AspLEI GCGC 3 cut(s) 238, 290, 2101
AspS9I GGNCC 3 cut(s) 1427, 1640, 3226
AsuC2I CCSGG 2 cut(s) 2307, 2773
AsuHPI GGTGA 9 cut(s) 476, 1067, 1724, 2321, 2714, 2921, 3090, 3240, 3305
AvaI CYCGRG 2 cut(s) 770, 2055
AvaII GGWCC 2 cut(s) 1640, 3226
AvrII CCTAGG 2 cut(s) 442, 1444
AxyI CCTNAGG 1 cut(s) 474
BanI GGYRCC 2 cut(s) 1571, 2406
BarI GAAGNNNNNNTAC 6 cut(s) 1441, 1473, 2328, 2360, 2878, 2910
BbsI GAAGAC 1 cut(s) 2893
Bbv12I GWGCWC 2 cut(s) 1755, 2541
BbvI GCAGC 6 cut(s) 203, 225, 348, 2016, 2505, 2973
BcgI CGANNNNNNTGC 2 cut(s) 92, 126
BciT130I CCWGG 4 cut(s) 233, 1053, 1820, 2456
BciVI GTATCC 1 cut(s) 1231
BcnI CCSGG 2 cut(s) 2307, 2773
BcoDI GTCTC 5 cut(s) 522, 1364, 1973, 2215, 3292
BcuI ACTAGT 5 cut(s) 206, 431, 1166, 1964, 2320
BfmI CTRYAG 4 cut(s) 313, 408, 2088, 3267
BfoI RGCGCY 1 cut(s) 239
BfrI CTTAAG 2 cut(s) 1382, 2150
BfuAI ACCTGC 2 cut(s) 378, 2032
BfuI GTATCC 1 cut(s) 1231
BisI GCNGC 9 cut(s) 192, 239, 337, 776, 1323, 1411, 2005, 2519, 2987
BlnI CCTAGG 2 cut(s) 442, 1444
BlsI GCNGC 9 cut(s) 193, 240, 338, 777, 1324, 1412, 2006, 2520, 2988
Bme1390I CCNGG 6 cut(s) 233, 1053, 1820, 2307, 2456, 2773
Bme18I GGWCC 2 cut(s) 1640, 3226
BmeRI GACNNNNNGTC 1 cut(s) 177
BmeT110I CYCGRG 2 cut(s) 770, 2055
BmgBI CACGTC 2 cut(s) 110, 179
BmgT120I GGNCC 3 cut(s) 1427, 1640, 3226
BmiI GGNNCC 6 cut(s) 682, 1069, 1428, 1573, 2408, 2723
BmrFI CCNGG 6 cut(s) 233, 1053, 1820, 2307, 2456, 2773
BmrI ACTGGG 2 cut(s) 553, 1121
BmsI GCATC 7 cut(s) 259, 2369, 2530, 2940, 2995, 3057, 3112
BmuI ACTGGG 2 cut(s) 553, 1121
BpiI GAAGAC 1 cut(s) 2893
BpmI CTGGAG 2 cut(s) 1193, 2787
Bpu10I CCTNAGC 1 cut(s) 1989
BpuEI CTTGAG 3 cut(s) 249, 602, 2425
BpuMI CCSGG 2 cut(s) 2307, 2773
BsaBI GATNNNNATC 1 cut(s) 3159
BsaHI GRCGYC 1 cut(s) 649
BsaI GGTCTC 1 cut(s) 3292
BsaWI WCCGGW 2 cut(s) 1593, 1637
BsaXI ACNNNNNCTCC 4 cut(s) 2029, 2059, 2811, 2841
Bsc4I CCNNNNNNNGG 9 cut(s) 88, 410, 914, 1517, 1943, 2312, 2731, 2810, 2965
Bse118I RCCGGY 1 cut(s) 1637
Bse1I ACTGG 5 cut(s) 559, 682, 1045, 1116, 2171
Bse21I CCTNAGG 1 cut(s) 474
Bse3DI GCAATG 1 cut(s) 2981
Bse8I GATNNNNATC 1 cut(s) 3159
BseAI TCCGGA 1 cut(s) 1593
BseBI CCWGG 4 cut(s) 233, 1053, 1820, 2456
BseJI GATNNNNATC 1 cut(s) 3159
BseLI CCNNNNNNNGG 9 cut(s) 88, 410, 914, 1517, 1943, 2312, 2731, 2810, 2965
BseMI GCAATG 1 cut(s) 2981
BseMII CTCAG 3 cut(s) 488, 2232, 3069
BseNI ACTGG 5 cut(s) 559, 682, 1045, 1116, 2171
BseRI GAGGAG 1 cut(s) 1221
BseXI GCAGC 6 cut(s) 203, 225, 348, 2016, 2505, 2973
BshFI GGCC 6 cut(s) 918, 1322, 1429, 2158, 2735, 2823
BshNI GGYRCC 2 cut(s) 1571, 2406
BshTI ACCGGT 1 cut(s) 1637
BsiHKAI GWGCWC 2 cut(s) 1755, 2541
BsiHKCI CYCGRG 2 cut(s) 770, 2055
BsiSI CCGG 4 cut(s) 1594, 1638, 2307, 2772
BslFI GGGAC 4 cut(s) 1274, 1279, 1426, 2288
BslI CCNNNNNNNGG 9 cut(s) 88, 410, 914, 1517, 1943, 2312, 2731, 2810, 2965
BsmAI GTCTC 5 cut(s) 522, 1364, 1973, 2215, 3292
BsmFI GGGAC 4 cut(s) 1274, 1279, 1426, 2288
BsmI GAATGC 6 cut(s) 4, 103, 2387, 2925, 3056, 3117
BsnI GGCC 6 cut(s) 918, 1322, 1429, 2158, 2735, 2823
Bso31I GGTCTC 1 cut(s) 3292
BsoBI CYCGRG 2 cut(s) 770, 2055
Bsp1286I GDGCHC 2 cut(s) 1755, 2541
Bsp13I TCCGGA 1 cut(s) 1593
Bsp143I GATC 8 cut(s) 226, 376, 727, 766, 1431, 1831, 2424, 2743
Bsp19I CCATGG 2 cut(s) 2943, 2964
BspACI CCGC 7 cut(s) 534, 735, 775, 954, 1323, 1410, 2601
BspANI GGCC 6 cut(s) 918, 1322, 1429, 2158, 2735, 2823
BspCNI CTCAG 3 cut(s) 487, 2231, 3068
BspEI TCCGGA 1 cut(s) 1593
BspHI TCATGA 1 cut(s) 282
BspLI GGNNCC 6 cut(s) 682, 1069, 1428, 1573, 2408, 2723
BspMAI CTGCAG 1 cut(s) 317
BspMI ACCTGC 2 cut(s) 378, 2032
BspPI GGATC 1 cut(s) 1426
BspT107I GGYRCC 2 cut(s) 1571, 2406
BspTI CTTAAG 2 cut(s) 1382, 2150
BspTNI GGTCTC 1 cut(s) 3292
BsrDI GCAATG 1 cut(s) 2981
BsrFI RCCGGY 1 cut(s) 1637
BsrI ACTGG 5 cut(s) 559, 682, 1045, 1116, 2171
BssAI RCCGGY 1 cut(s) 1637
BssMI GATC 8 cut(s) 226, 376, 727, 766, 1431, 1831, 2424, 2743
BssNAI GTATAC 1 cut(s) 2168
BssNI GRCGYC 1 cut(s) 649
BssT1I CCWWGG 7 cut(s) 442, 1444, 1729, 2159, 2836, 2943, 2964
Bst1107I GTATAC 1 cut(s) 2168
Bst2UI CCWGG 4 cut(s) 233, 1053, 1820, 2456
Bst4CI ACNGT 5 cut(s) 198, 2411, 2549, 3252, 3271
Bst6I CTCTTC 1 cut(s) 930
BstACI GRCGYC 1 cut(s) 649
BstAFI CTTAAG 2 cut(s) 1382, 2150
BstC8I GCNNGC 8 cut(s) 125, 325, 391, 1129, 2029, 2067, 2103, 2437
BstDSI CCRYGG 2 cut(s) 2943, 2964
BstENI CCTNNNNNAGG 3 cut(s) 408, 1941, 2729
BstH2I RGCGCY 1 cut(s) 239
BstHHI GCGC 3 cut(s) 238, 290, 2101
BstKTI GATC 8 cut(s) 229, 379, 730, 769, 1434, 1834, 2427, 2746
BstMAI GTCTC 5 cut(s) 522, 1364, 1973, 2215, 3292
BstMBI GATC 8 cut(s) 226, 376, 727, 766, 1431, 1831, 2424, 2743
BstMWI GCNNNNNNNGC 6 cut(s) 333, 655, 898, 1468, 2155, 2820
BstNI CCWGG 4 cut(s) 233, 1053, 1820, 2456
BstNSI RCATGY 7 cut(s) 336, 722, 951, 1621, 2031, 2439, 2908
BstSCI CCNGG 6 cut(s) 231, 1051, 1818, 2305, 2454, 2771
BstSFI CTRYAG 4 cut(s) 313, 408, 2088, 3267
BstV1I GCAGC 6 cut(s) 203, 225, 348, 2016, 2505, 2973
BstV2I GAAGAC 1 cut(s) 2893
BstXI CCANNNNNNTGG 2 cut(s) 181, 893
BstZ17I GTATAC 1 cut(s) 2168
Bsu36I CCTNAGG 1 cut(s) 474
BsuI GTATCC 1 cut(s) 1231
BsuRI GGCC 6 cut(s) 918, 1322, 1429, 2158, 2735, 2823
BtgI CCRYGG 2 cut(s) 2943, 2964
BtgZI GCGATG 1 cut(s) 2963
BtrI CACGTC 2 cut(s) 110, 179
BtsI GCAGTG 1 cut(s) 2937
BtsIMutI CAGTG 6 cut(s) 194, 566, 1357, 2937, 3069, 3276
BveI ACCTGC 2 cut(s) 378, 2032
Cac8I GCNNGC 8 cut(s) 125, 325, 391, 1129, 2029, 2067, 2103, 2437
CciI TCATGA 1 cut(s) 282
CfoI GCGC 3 cut(s) 238, 290, 2101
Cfr10I RCCGGY 1 cut(s) 1637
Cfr13I GGNCC 3 cut(s) 1427, 1640, 3226
CseI GACGC 3 cut(s) 586, 638, 1276
Csp6I GTAC 3 cut(s) 564, 1164, 2488
CspAI ACCGGT 1 cut(s) 1637
CspCI CAANNNNNGTGG 4 cut(s) 1711, 1746, 2606, 2641
CviQI GTAC 3 cut(s) 564, 1164, 2488
DpnI GATC 8 cut(s) 228, 378, 729, 768, 1433, 1833, 2426, 2745
DpnII GATC 8 cut(s) 226, 376, 727, 766, 1431, 1831, 2424, 2743
DraI TTTAAA 2 cut(s) 139, 2911
DriI GACNNNNNGTC 1 cut(s) 177
EaeI YGGCCR 1 cut(s) 1320
Eam1104I CTCTTC 1 cut(s) 930
Eam1105I GACNNNNNGTC 1 cut(s) 177
EarI CTCTTC 1 cut(s) 930
Eco130I CCWWGG 7 cut(s) 442, 1444, 1729, 2159, 2836, 2943, 2964
Eco147I AGGCCT 1 cut(s) 2735
Eco31I GGTCTC 1 cut(s) 3292
Eco47I GGWCC 2 cut(s) 1640, 3226
Eco57I CTGAAG 4 cut(s) 514, 1386, 1959, 2441
Eco81I CCTNAGG 1 cut(s) 474
Eco88I CYCGRG 2 cut(s) 770, 2055
EcoNI CCTNNNNNAGG 3 cut(s) 408, 1941, 2729
EcoRI GAATTC 1 cut(s) 114
EcoRII CCWGG 4 cut(s) 231, 1051, 1818, 2454
EcoT14I CCWWGG 7 cut(s) 442, 1444, 1729, 2159, 2836, 2943, 2964
EcoT22I ATGCAT 2 cut(s) 6, 170
ErhI CCWWGG 7 cut(s) 442, 1444, 1729, 2159, 2836, 2943, 2964
FalI AAGNNNNNCTT 3 cut(s) 37, 1276, 1308
FaqI GGGAC 4 cut(s) 1274, 1279, 1426, 2288
FauI CCCGC 1 cut(s) 947
FblI GTMKAC 2 cut(s) 1534, 2167
Fnu4HI GCNGC 9 cut(s) 192, 239, 337, 776, 1323, 1411, 2005, 2519, 2987
Fsp4HI GCNGC 9 cut(s) 192, 239, 337, 776, 1323, 1411, 2005, 2519, 2987
GlaI GCGC 3 cut(s) 237, 289, 2100
GluI GCNGC 9 cut(s) 192, 239, 337, 776, 1323, 1411, 2005, 2519, 2987
GsuI CTGGAG 2 cut(s) 1193, 2787
HaeII RGCGCY 1 cut(s) 239
HaeIII GGCC 6 cut(s) 918, 1322, 1429, 2158, 2735, 2823
HapII CCGG 4 cut(s) 1594, 1638, 2307, 2772
HgaI GACGC 3 cut(s) 586, 638, 1276
HhaI GCGC 3 cut(s) 238, 290, 2101
Hin1I GRCGYC 1 cut(s) 649
Hin6I GCGC 3 cut(s) 236, 288, 2099
HinP1I GCGC 3 cut(s) 236, 288, 2099
HincII GTYRAC 2 cut(s) 1635, 2977
HindII GTYRAC 2 cut(s) 1635, 2977
HindIII AAGCTT 3 cut(s) 1078, 2388, 2503
HinfI GANTC 9 cut(s) 299, 354, 613, 1513, 1861, 1947, 2574, 3078, 3155
HpaII CCGG 4 cut(s) 1594, 1638, 2307, 2772
HphI GGTGA 9 cut(s) 476, 1067, 1724, 2321, 2714, 2921, 3090, 3240, 3305
Hpy166II GTNNAC 9 cut(s) 564, 1535, 1635, 1643, 2168, 2290, 2880, 2977, 3133
Hpy188I TCNGA 8 cut(s) 675, 800, 1512, 1654, 2221, 2262, 2421, 2526
Hpy8I GTNNAC 9 cut(s) 564, 1535, 1635, 1643, 2168, 2290, 2880, 2977, 3133
Hpy99I CGWCG 3 cut(s) 114, 1441, 2996
HpyAV CCTTC 6 cut(s) 414, 538, 1937, 2116, 2354, 3033
HpyCH4III ACNGT 5 cut(s) 198, 2411, 2549, 3252, 3271
HpyCH4IV ACGT 6 cut(s) 109, 178, 504, 866, 1531, 2182
HpyF10VI GCNNNNNNNGC 6 cut(s) 333, 655, 898, 1468, 2155, 2820
HpySE526I ACGT 6 cut(s) 109, 178, 504, 866, 1531, 2182
Hsp92I GRCGYC 1 cut(s) 649
HspAI GCGC 3 cut(s) 236, 288, 2099
Kpn2I TCCGGA 1 cut(s) 1593
Kzo9I GATC 8 cut(s) 226, 376, 727, 766, 1431, 1831, 2424, 2743
LmnI GCTCC 3 cut(s) 100, 1681, 2357
Lsp1109I GCAGC 6 cut(s) 203, 225, 348, 2016, 2505, 2973
LweI GCATC 7 cut(s) 259, 2369, 2530, 2940, 2995, 3057, 3112
MaeII ACGT 6 cut(s) 109, 178, 504, 866, 1531, 2182
MaeIII GTNAC 6 cut(s) 25, 328, 590, 1448, 1556, 2702
MalI GATC 8 cut(s) 228, 378, 729, 768, 1433, 1833, 2426, 2745
MboI GATC 8 cut(s) 226, 376, 727, 766, 1431, 1831, 2424, 2743
MfeI CAATTG 2 cut(s) 2586, 2637
MhlI GDGCHC 2 cut(s) 1755, 2541
MlyI GAGTC 4 cut(s) 363, 1522, 2568, 3072
MmeI TCCRAC 4 cut(s) 569, 674, 778, 1639
Mph1103I ATGCAT 2 cut(s) 6, 170
MroI TCCGGA 1 cut(s) 1593
MroXI GAANNNNTTC 5 cut(s) 418, 706, 962, 1951, 2226
MslI CAYNNNNRTG 4 cut(s) 2625, 2700, 2942, 2948
MspA1I CMGCKG 1 cut(s) 534
MspCI CTTAAG 2 cut(s) 1382, 2150
MspI CCGG 4 cut(s) 1594, 1638, 2307, 2772
MspR9I CCNGG 6 cut(s) 233, 1053, 1820, 2307, 2456, 2773
MssI GTTTAAAC 1 cut(s) 139
MunI CAATTG 2 cut(s) 2586, 2637
Mva1269I GAATGC 6 cut(s) 4, 103, 2387, 2925, 3056, 3117
MvaI CCWGG 4 cut(s) 233, 1053, 1820, 2456
MwoI GCNNNNNNNGC 6 cut(s) 333, 655, 898, 1468, 2155, 2820
NciI CCSGG 2 cut(s) 2307, 2773
NcoI CCATGG 2 cut(s) 2943, 2964
NdeII GATC 8 cut(s) 226, 376, 727, 766, 1431, 1831, 2424, 2743
NlaIV GGNNCC 6 cut(s) 682, 1069, 1428, 1573, 2408, 2723
NmuCI GTSAC 1 cut(s) 2702
NsiI ATGCAT 2 cut(s) 6, 170
NspI RCATGY 7 cut(s) 336, 722, 951, 1621, 2031, 2439, 2908
OliI CACNNNNGTG 1 cut(s) 2625
PaeI GCATGC 2 cut(s) 2031, 2439
PaeR7I CTCGAG 1 cut(s) 2055
PagI TCATGA 1 cut(s) 282
PceI AGGCCT 1 cut(s) 2735
PciI ACATGT 1 cut(s) 2904
PctI GAATGC 6 cut(s) 4, 103, 2387, 2925, 3056, 3117
PdmI GAANNNNTTC 5 cut(s) 418, 706, 962, 1951, 2226
PfeI GAWTC 5 cut(s) 299, 613, 1861, 1947, 3155
PflMI CCANNNNNTGG 2 cut(s) 2810, 2965
PinAI ACCGGT 1 cut(s) 1637
PkrI GCNGC 9 cut(s) 193, 240, 338, 777, 1324, 1412, 2006, 2520, 2988
PleI GAGTC 4 cut(s) 362, 1521, 2568, 3072
PmeI GTTTAAAC 1 cut(s) 139
PpsI GAGTC 4 cut(s) 362, 1521, 2568, 3072
PscI ACATGT 1 cut(s) 2904
PsiI TTATAA 1 cut(s) 2190
Psp6I CCWGG 4 cut(s) 231, 1051, 1818, 2454
PspGI CCWGG 4 cut(s) 231, 1051, 1818, 2454
PspN4I GGNNCC 6 cut(s) 682, 1069, 1428, 1573, 2408, 2723
PspPI GGNCC 3 cut(s) 1427, 1640, 3226
PstI CTGCAG 1 cut(s) 317
RsaI GTAC 3 cut(s) 565, 1165, 2489
RsaNI GTAC 3 cut(s) 564, 1164, 2488
RseI CAYNNNNRTG 4 cut(s) 2625, 2700, 2942, 2948
SatI GCNGC 9 cut(s) 192, 239, 337, 776, 1323, 1411, 2005, 2519, 2987
Sau3AI GATC 8 cut(s) 226, 376, 727, 766, 1431, 1831, 2424, 2743
Sau96I GGNCC 3 cut(s) 1427, 1640, 3226
SchI GAGTC 4 cut(s) 363, 1522, 2568, 3072
ScrFI CCNGG 6 cut(s) 233, 1053, 1820, 2307, 2456, 2773
SduI GDGCHC 2 cut(s) 1755, 2541
SfaNI GCATC 7 cut(s) 259, 2369, 2530, 2940, 2995, 3057, 3112
SfcI CTRYAG 4 cut(s) 313, 408, 2088, 3267
Sfr274I CTCGAG 1 cut(s) 2055
SinI GGWCC 2 cut(s) 1640, 3226
SlaI CTCGAG 1 cut(s) 2055
SmiMI CAYNNNNRTG 4 cut(s) 2625, 2700, 2942, 2948
SmlI CTYRAG 6 cut(s) 264, 581, 1382, 2055, 2150, 2440
SmoI CTYRAG 6 cut(s) 264, 581, 1382, 2055, 2150, 2440
SpeI ACTAGT 5 cut(s) 206, 431, 1166, 1964, 2320
SphI GCATGC 2 cut(s) 2031, 2439
SseBI AGGCCT 1 cut(s) 2735
SsiI CCGC 7 cut(s) 534, 735, 775, 954, 1323, 1410, 2601
SspI AATATT 3 cut(s) 850, 976, 2581
StuI AGGCCT 1 cut(s) 2735
StyD4I CCNGG 6 cut(s) 231, 1051, 1818, 2305, 2454, 2771
StyI CCWWGG 7 cut(s) 442, 1444, 1729, 2159, 2836, 2943, 2964
TaaI ACNGT 5 cut(s) 198, 2411, 2549, 3252, 3271
TaiI ACGT 6 cut(s) 112, 181, 507, 869, 1534, 2185
TaqI TCGA 9 cut(s) 112, 243, 611, 1838, 2009, 2056, 2071, 2994, 3287
TatI WGTACW 2 cut(s) 1163, 2487
TauI GCSGC 3 cut(s) 778, 1325, 1413
TfiI GAWTC 5 cut(s) 299, 613, 1861, 1947, 3155
TscAI CASTG 6 cut(s) 201, 566, 1357, 2944, 3069, 3276
TseFI GTSAC 1 cut(s) 2702
TseI GCWGC 6 cut(s) 191, 238, 336, 2004, 2518, 2986
Tsp45I GTSAC 1 cut(s) 2702
TspGWI ACGGA 1 cut(s) 71
TspRI CASTG 6 cut(s) 201, 566, 1357, 2944, 3069, 3276
Van91I CCANNNNNTGG 2 cut(s) 2810, 2965
Vha464I CTTAAG 2 cut(s) 1382, 2150
VpaK11BI GGWCC 2 cut(s) 1640, 3226
XagI CCTNNNNNAGG 3 cut(s) 408, 1941, 2729
XbaI TCTAGA 1 cut(s) 2249
XceI RCATGY 7 cut(s) 336, 722, 951, 1621, 2031, 2439, 2908
XcmI CCANNNNNNNNNTGG 2 cut(s) 2371, 3236
XhoI CTCGAG 1 cut(s) 2055
XmaJI CCTAGG 2 cut(s) 442, 1444
XmiI GTMKAC 2 cut(s) 1534, 2167
XmnI GAANNNNTTC 5 cut(s) 418, 706, 962, 1951, 2226
Zsp2I ATGCAT 2 cut(s) 6, 170
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.