MD05G1075800.v1.1

LRR receptor-like serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0000262
Physical Location & Seq
Reverse (-)
16281964 .. 16283752
1789 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1075800.v1.1.491

Sequence Viewer

Length: 1341 bp
ATGACTTTAGTGTTAAACCTCCACATGGGGTTAAACGGGTTTGAGAGCAATATACCTCCAAGTCTTGGAAACTACCGAAACATTTTAGAATTTAACCTTTCAAGTAACAACCTTATGGGCACCATACCTAAAATGCTTATGGAGCTTTCAACCCTTTCAATTTCTTTAGACCTGTCTGACAATTATTTGACTGGTCTGCTGCCCTTTGAGAGCAACCTTGGTAGTTGTACTAGTTTGGAGCACTTGTATTTGCAAGGTAATAAGTTTGAAGGAACAATTCCTCAATCTCTTAAAGATTTAAAAGGCTTGGAAATACTTGATATTTCAAGCAACAACTTGTCTAGGCAGATTCCTGAATTCATAGGCAAGCTTGGTGCTCTCAAGTATCCCAATCTTTTGTATAATGATTTTGAGGGTGAGTTGCCTAAAGAAGGTATTTTTGCAAATGCTGGTGGTGTGTTTGTTCTTGGAAATCATGGGCTGTGTGGTGGCATCCCACAATTTTATCTACCTCCAGGCCCCCCTAAAAAGTTCCATTCATCTCGAGGACCGCTTTCTCCCAAAGTAGTCATTCCTACAGCTTGTACACTTGCAATCGTAATTGCTCTATCATGCTTCTTTGTTGCTTGTTCAATGCTGAAAAAGTCAAGAGATGGACTTATAACTTTGCGTTCTTATATGGGTTGGAAATTAGGTGTCTCCTACTCACAACTCGTTGAATCAACTAACAGGTTCTCTATGAATAATCTTATTGGTTCGGGAAGTTTTGGTTCTGTTTATAAAGGGGTAATTCCTAGTGATGGAAGGATAGTTGCTGTTAAGGAGCAATCTCTAAGTAACAGATTGAGCGTTATTCGAAGATTGGATGTTGCCATTGGTGTTGCTTATGCGTTAGATTATCTCCATCACCATTGTGAAACAACTATTGTTCATTACGATTTAAAGCCGAGCAACGTACTTCTCGATGAAGATATGGTAGCCCATGTTGGGGATTTTAGTTTAGGAAGGTTCCTTTTCGAAACATCAAATGATCCTTCCTTTAGTCAAACCATGTCATCTCAGCTAAAAGGTTCTATAGGCTACATTCCTCCAGAGTATGGCACGGGAGGCCAAGTTTCCACACTTGGAGGTGTTTATAGCTATGAGATACTGTTGCTGGAAATATTTACAGGAAAAAGACCTACTGATGACATGTTCAAAAACGATCTAAGCATTTACCAATTCATAGTCATGGCTTTGCCTAGCCATGTGATGGACGTTGTTAACCTTTCAATTCTGGTCGACCTCGAAGCAGATGGTGATGTTAACGATGACATAGTACAAGAACAAACTCCATCTTGA

Protein Analysis

447

Amino Acids

48.79

Weight (kDa)

5.22

Isoelectric Point (pI)

40.02

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRR_4 PF12799 77 - 120 1.4e-06 Leucine Rich repeats (2 copies)
LRR_8 PF13855 78 - 136 2.5e-06 Leucine rich repeat
PK_Tyr_Ser-Thr PF07714 279 - 402 4.4e-16 Protein tyrosine and serine/threonine kinase
Pkinase PF00069 292 - 397 2.3e-14 Protein kinase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000262)

Species Orthologous Gene IDs
fragaria_vesca FvH4_6g11680 FvH4_6g11680 FvH4_6g11680 FvH4_6g11680 FvH4_6g11680 FvH4_6g11680 FvH4_6g11681 FvH4_6g11682 FvH4_6g11683
malus_domestica MD00G1079100.v1.1 MD00G1080000.v1.1 MD01G1100900.v1.1 MD03G1225200.v1.1 MD04G1041600.v1.1 MD04G1110500.v1.1 MD04G1118700.v1.1 MD04G1150300.v1.1 MD04G1150400.v1.1 MD04G1156000.v1.1 MD04G1156200.v1.1 MD04G1156700.v1.1 MD04G1157400.v1.1 MD04G1157500.v1.1 MD04G1157700.v1.1 MD04G1158000.v1.1 MD04G1158200.v1.1 MD04G1158600.v1.1 MD04G1159000.v1.1 MD04G1159100.v1.1 MD05G1061600.v1.1 MD05G1075800.v1.1 MD06G1129300.v1.1 MD07G1217800.v1.1 MD07G1218000.v1.1 MD07G1308700.v1.1 MD12G1130700.v1.1 MD12G1130800.v1.1 MD12G1134500.v1.1 MD12G1134700.v1.1 MD12G1135000.v1.1 MD12G1135200.v1.1 MD12G1135800.v1.1 MD12G1164300.v1.1 MD12G1164400.v1.1 MD12G1169100.v1.1 MD12G1169700.v1.1 MD12G1170200.v1.1 MD12G1170400.v1.1 MD13G1275300.v1.1
prunus_persica Prupe.1G072900_v2.0.a1 Prupe.1G194200_v2.0.a1 Prupe.1G201100_v2.0.a1 Prupe.6G248000_v2.0.a1 Prupe.6G248100_v2.0.a1 Prupe.6G248500_v2.0.a1 Prupe.6G274800_v2.0.a1 Prupe.6G274900_v2.0.a1 Prupe.6G275100_v2.0.a1 Prupe.6G275100_v2.0.a1 Prupe.7G044200_v2.0.a1 Prupe.7G044300_v2.0.a1 Prupe.7G044700_v2.0.a1
pyrus_communis pycom04g03530 pycom04g10490 pycom04g10500 pycom04g10510 pycom04g10560 pycom04g13580 pycom04g13600 pycom04g14080 pycom12g10580 pycom12g11020 pycom12g12650 pycom12g12680 pycom12g15730 pycom12g15740 pycom12g16060 pycom12g16100 pycom12g16200 pycom12g16240 pycom16g21060
rosa_chinensis RchiOBHm_Chr3g0463401 RchiOBHm_Chr3g0463411 RchiOBHm_Chr3g0463481 RchiOBHm_Chr4g0408571 RchiOBHm_Chr4g0408641
rosa_laevigata RLG00000019546 RLG00000019547 RLG00000019552 RLG00000019553 RLG00000019554 RLG00000019578 RLG00000024784 RLG00000024785 RLG00000024786
rosa_multiflora Rmu_sc0000923.1_g000013 Rmu_sc0000923.1_g000017 Rmu_sc0000923.1_g000018 Rmu_sc0001053.1_g000005 Rmu_sc0001053.1_g000012 Rmu_sc0001053.1_g000013 Rmu_sc0001053.1_g000014 Rmu_sc0031971.1_g000001
rosa_roxburghii Rroxscaffold_2G00107980 Rroxscaffold_6G00416540 Rroxscaffold_6G00416560
rosa_rugosa Rorug02G0333800 Rorug02G0333900 Rorug03G0064500 Rorug03G0064600 Rorug03G0064800 Rorug03G0064900 Rorug03G0065000 Rorug03G0065000
rosa_samantha Rh2CG370900 Rh3AG122100 Rh3BG126500 Rh3BG126800 Rh3BG126900 Rh3CG128600 Rh3CG128700 Rh3DG127400 Rh3DG127500 Rh3DG127700 Rh4CG158500 Rh4CG158600
rosa_wichuraiana Rw0G010610 Rw0G010620 Rw3G010370 Rw3G010380 Rw3G010400 Rw3G010410

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 662, 780
AccB1I GGYRCC 1 cut(s) 119
AccB7I CCANNNNNTGG 3 cut(s) 65, 1097, 1252
AccI GTMKAC 1 cut(s) 1281
AciI CCGC 1 cut(s) 551
AclWI GGATC 1 cut(s) 1025
AcsI RAATTY 2 cut(s) 89, 356
AfaI GTAC 4 cut(s) 229, 586, 957, 1320
AfiI CCNNNNNNNGG 8 cut(s) 25, 65, 431, 800, 987, 988, 1097, 1252
AflIII ACRYGT 1 cut(s) 1191
AgsI TTSAA 9 cut(s) 102, 150, 159, 269, 327, 633, 719, 1198, 1272
AhlI ACTAGT 1 cut(s) 230
AjnI CCWGG 1 cut(s) 514
AleI CACNNNNGTG 1 cut(s) 912
AluBI AGCT 5 cut(s) 145, 370, 581, 1063, 1140
AluI AGCT 5 cut(s) 145, 370, 581, 1063, 1140
Alw21I GWGCWC 2 cut(s) 243, 379
Alw26I GTCTC 1 cut(s) 703
AlwI GGATC 1 cut(s) 1025
Ama87I CYCGRG 1 cut(s) 543
AoxI GGCC 2 cut(s) 517, 1108
ApeKI GCWGC 1 cut(s) 199
ApoI RAATTY 2 cut(s) 89, 356
AspS9I GGNCC 2 cut(s) 518, 548
AsuHPI GGTGA 3 cut(s) 428, 899, 1310
AsuII TTCGAA 2 cut(s) 856, 1017
AvaI CYCGRG 1 cut(s) 543
AvaII GGWCC 1 cut(s) 548
BaeGI GKGCMC 1 cut(s) 122
BanI GGYRCC 1 cut(s) 119
Bbv12I GWGCWC 2 cut(s) 243, 379
BbvI GCAGC 1 cut(s) 186
BccI CCATC 5 cut(s) 647, 794, 912, 1246, 1289
BciT130I CCWGG 1 cut(s) 516
BciVI GTATCC 1 cut(s) 396
BcoDI GTCTC 1 cut(s) 703
BcuI ACTAGT 1 cut(s) 230
BfaI CTAG 4 cut(s) 231, 342, 795, 1242
BfmI CTRYAG 2 cut(s) 576, 1074
BfuI GTATCC 1 cut(s) 396
BisI GCNGC 1 cut(s) 200
BlsI GCNGC 1 cut(s) 201
Bme1390I CCNGG 1 cut(s) 516
Bme18I GGWCC 1 cut(s) 548
BmeT110I CYCGRG 1 cut(s) 543
BmgT120I GGNCC 2 cut(s) 518, 548
BmiI GGNNCC 3 cut(s) 121, 520, 1010
BmrFI CCNGG 1 cut(s) 516
BmsI GCATC 1 cut(s) 501
BpmI CTGGAG 2 cut(s) 498, 1074
Bpu14I TTCGAA 2 cut(s) 856, 1017
BpuEI CTTGAG 1 cut(s) 365
BsaJI CCNNGG 1 cut(s) 217
BsaXI ACNNNNNCTCC 4 cut(s) 230, 260, 1098, 1128
Bsc4I CCNNNNNNNGG 8 cut(s) 25, 65, 431, 800, 987, 988, 1097, 1252
Bse1I ACTGG 1 cut(s) 196
BseBI CCWGG 1 cut(s) 516
BseDI CCNNGG 1 cut(s) 217
BseGI GGATG 2 cut(s) 492, 871
BseLI CCNNNNNNNGG 8 cut(s) 25, 65, 431, 800, 987, 988, 1097, 1252
BseMII CTCAG 1 cut(s) 1073
BseNI ACTGG 1 cut(s) 196
BseSI GKGCMC 1 cut(s) 122
BseXI GCAGC 1 cut(s) 186
BshFI GGCC 2 cut(s) 519, 1110
BshNI GGYRCC 1 cut(s) 119
BsiHKAI GWGCWC 2 cut(s) 243, 379
BsiHKCI CYCGRG 1 cut(s) 543
BslI CCNNNNNNNGG 8 cut(s) 25, 65, 431, 800, 987, 988, 1097, 1252
BsmAI GTCTC 1 cut(s) 703
BsnI GGCC 2 cut(s) 519, 1110
BsoBI CYCGRG 1 cut(s) 543
Bsp119I TTCGAA 2 cut(s) 856, 1017
Bsp1286I GDGCHC 3 cut(s) 122, 243, 379
Bsp1407I TGTACA 1 cut(s) 584
Bsp143I GATC 2 cut(s) 1030, 1204
BspACI CCGC 1 cut(s) 551
BspANI GGCC 2 cut(s) 519, 1110
BspCNI CTCAG 1 cut(s) 1072
BspLI GGNNCC 3 cut(s) 121, 520, 1010
BspPI GGATC 1 cut(s) 1025
BspT104I TTCGAA 2 cut(s) 856, 1017
BspT107I GGYRCC 1 cut(s) 119
BsrGI TGTACA 1 cut(s) 584
BsrI ACTGG 1 cut(s) 196
BssECI CCNNGG 1 cut(s) 217
BssMI GATC 2 cut(s) 1030, 1204
BssT1I CCWWGG 1 cut(s) 217
Bst2UI CCWGG 1 cut(s) 516
Bst4CI ACNGT 1 cut(s) 1152
BstAUI TGTACA 1 cut(s) 584
BstBI TTCGAA 2 cut(s) 856, 1017
BstC8I GCNNGC 1 cut(s) 368
BstDEI CTNAG 3 cut(s) 833, 1059, 1208
BstENI CCTNNNNNAGG 1 cut(s) 429
BstF5I GGATG 2 cut(s) 492, 871
BstKTI GATC 2 cut(s) 1033, 1207
BstMAI GTCTC 1 cut(s) 703
BstMBI GATC 2 cut(s) 1030, 1204
BstMWI GCNNNNNNNGC 2 cut(s) 142, 1107
BstNI CCWGG 1 cut(s) 516
BstNSI RCATGY 1 cut(s) 1195
BstSCI CCNGG 1 cut(s) 514
BstSFI CTRYAG 2 cut(s) 576, 1074
BstSLI GKGCMC 1 cut(s) 122
BstV1I GCAGC 1 cut(s) 186
BsuI GTATCC 1 cut(s) 396
BsuRI GGCC 2 cut(s) 519, 1110
BtsCI GGATG 2 cut(s) 492, 871
Cac8I GCNNGC 1 cut(s) 368
Cfr13I GGNCC 2 cut(s) 518, 548
Csp6I GTAC 4 cut(s) 228, 585, 956, 1319
CviAII CATG 8 cut(s) 25, 476, 612, 983, 1051, 1192, 1231, 1247
CviQI GTAC 4 cut(s) 228, 585, 956, 1319
DdeI CTNAG 3 cut(s) 833, 1059, 1208
DpnI GATC 2 cut(s) 1032, 1206
DpnII GATC 2 cut(s) 1030, 1204
DraI TTTAAA 2 cut(s) 300, 942
Eco130I CCWWGG 1 cut(s) 217
Eco47I GGWCC 1 cut(s) 548
Eco88I CYCGRG 1 cut(s) 543
EcoNI CCTNNNNNAGG 1 cut(s) 429
EcoO109I RGGNCCY 1 cut(s) 518
EcoRI GAATTC 1 cut(s) 356
EcoRII CCWGG 1 cut(s) 514
EcoT14I CCWWGG 1 cut(s) 217
ErhI CCWWGG 1 cut(s) 217
FaeI CATG 8 cut(s) 28, 479, 615, 986, 1054, 1195, 1234, 1250
FatI CATG 8 cut(s) 24, 475, 611, 982, 1050, 1191, 1230, 1246
FblI GTMKAC 1 cut(s) 1281
Fnu4HI GCNGC 1 cut(s) 200
FokI GGATG 2 cut(s) 479, 878
Fsp4HI GCNGC 1 cut(s) 200
FspBI CTAG 4 cut(s) 231, 342, 795, 1242
GluI GCNGC 1 cut(s) 200
GsuI CTGGAG 2 cut(s) 498, 1074
HaeIII GGCC 2 cut(s) 519, 1110
Hin1II CATG 8 cut(s) 28, 479, 615, 986, 1054, 1195, 1234, 1250
HincII GTYRAC 3 cut(s) 1264, 1282, 1306
HindII GTYRAC 3 cut(s) 1264, 1282, 1306
HindIII AAGCTT 1 cut(s) 368
HinfI GANTC 2 cut(s) 349, 719
HpaI GTTAAC 2 cut(s) 1264, 1306
HphI GGTGA 3 cut(s) 428, 899, 1310
Hpy166II GTNNAC 4 cut(s) 587, 1264, 1282, 1306
Hpy188I TCNGA 1 cut(s) 178
Hpy188III TCNNGA 7 cut(s) 353, 543, 648, 759, 962, 1091, 1338
Hpy8I GTNNAC 4 cut(s) 587, 1264, 1282, 1306
HpyAV CCTTC 5 cut(s) 263, 425, 798, 999, 1044
HpyCH4III ACNGT 1 cut(s) 1152
HpyCH4IV ACGT 2 cut(s) 954, 1257
HpyCH4V TGCA 3 cut(s) 253, 443, 593
HpyF10VI GCNNNNNNNGC 2 cut(s) 142, 1107
HpyF3I CTNAG 3 cut(s) 833, 1059, 1208
HpySE526I ACGT 2 cut(s) 954, 1257
Hsp92II CATG 8 cut(s) 28, 479, 615, 986, 1054, 1195, 1234, 1250
KspAI GTTAAC 2 cut(s) 1264, 1306
Kzo9I GATC 2 cut(s) 1030, 1204
LmnI GCTCC 3 cut(s) 142, 238, 823
Lsp1109I GCAGC 1 cut(s) 186
LweI GCATC 1 cut(s) 501
MaeI CTAG 4 cut(s) 231, 342, 795, 1242
MaeII ACGT 2 cut(s) 954, 1257
MaeIII GTNAC 2 cut(s) 104, 836
MalI GATC 2 cut(s) 1032, 1206
MboI GATC 2 cut(s) 1030, 1204
MboII GAAGA 2 cut(s) 870, 980
MhlI GDGCHC 3 cut(s) 122, 243, 379
MmeI TCCRAC 1 cut(s) 665
MseI TTAA 9 cut(s) 14, 32, 93, 291, 299, 819, 941, 1263, 1305
MslI CAYNNNNRTG 2 cut(s) 912, 1229
MspR9I CCNGG 1 cut(s) 516
MvaI CCWGG 1 cut(s) 516
MwoI GCNNNNNNNGC 2 cut(s) 142, 1107
NdeII GATC 2 cut(s) 1030, 1204
NlaIII CATG 8 cut(s) 28, 479, 615, 986, 1054, 1195, 1234, 1250
NlaIV GGNNCC 3 cut(s) 121, 520, 1010
NmeAIII GCCGAG 1 cut(s) 972
NspI RCATGY 1 cut(s) 1195
NspV TTCGAA 2 cut(s) 856, 1017
OliI CACNNNNGTG 1 cut(s) 912
PaeR7I CTCGAG 1 cut(s) 543
PciI ACATGT 1 cut(s) 1191
PcsI WCGNNNNNNNCGW 1 cut(s) 960
PfeI GAWTC 2 cut(s) 349, 719
PflMI CCANNNNNTGG 3 cut(s) 65, 1097, 1252
PkrI GCNGC 1 cut(s) 201
PscI ACATGT 1 cut(s) 1191
PsiI TTATAA 2 cut(s) 662, 780
Psp6I CCWGG 1 cut(s) 514
PspGI CCWGG 1 cut(s) 514
PspN4I GGNNCC 3 cut(s) 121, 520, 1010
PspPI GGNCC 2 cut(s) 518, 548
RsaI GTAC 4 cut(s) 229, 586, 957, 1320
RsaNI GTAC 4 cut(s) 228, 585, 956, 1319
RseI CAYNNNNRTG 2 cut(s) 912, 1229
SalI GTCGAC 1 cut(s) 1280
SaqAI TTAA 9 cut(s) 14, 32, 93, 291, 299, 819, 941, 1263, 1305
SatI GCNGC 1 cut(s) 200
Sau3AI GATC 2 cut(s) 1030, 1204
Sau96I GGNCC 2 cut(s) 518, 548
ScrFI CCNGG 1 cut(s) 516
SduI GDGCHC 3 cut(s) 122, 243, 379
SfaNI GCATC 1 cut(s) 501
SfcI CTRYAG 2 cut(s) 576, 1074
Sfr274I CTCGAG 1 cut(s) 543
SfuI TTCGAA 2 cut(s) 856, 1017
SinI GGWCC 1 cut(s) 548
SlaI CTCGAG 1 cut(s) 543
SmiMI CAYNNNNRTG 2 cut(s) 912, 1229
SmlI CTYRAG 2 cut(s) 380, 543
SmoI CTYRAG 2 cut(s) 380, 543
SpeI ACTAGT 1 cut(s) 230
SsiI CCGC 1 cut(s) 551
SspI AATATT 1 cut(s) 1164
SspMI CTAG 4 cut(s) 231, 342, 795, 1242
StyD4I CCNGG 1 cut(s) 514
StyI CCWWGG 1 cut(s) 217
TaaI ACNGT 1 cut(s) 1152
TaiI ACGT 2 cut(s) 957, 1260
TaqI TCGA 6 cut(s) 544, 856, 963, 1017, 1281, 1287
TatI WGTACW 3 cut(s) 227, 584, 1318
TfiI GAWTC 2 cut(s) 349, 719
Tru1I TTAA 9 cut(s) 14, 32, 93, 291, 299, 819, 941, 1263, 1305
Tru9I TTAA 9 cut(s) 14, 32, 93, 291, 299, 819, 941, 1263, 1305
TseI GCWGC 1 cut(s) 199
TspDTI ATGAA 6 cut(s) 349, 528, 755, 920, 981, 1213
Van91I CCANNNNNTGG 3 cut(s) 65, 1097, 1252
VpaK11BI GGWCC 1 cut(s) 548
XagI CCTNNNNNAGG 1 cut(s) 429
XapI RAATTY 2 cut(s) 89, 356
XceI RCATGY 1 cut(s) 1195
XhoI CTCGAG 1 cut(s) 543
XmiI GTMKAC 1 cut(s) 1281
XspI CTAG 4 cut(s) 231, 342, 795, 1242
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.