LRR receptor-like serine threonine-protein kinase
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AanI | TTATAA | 1 cut(s) | 672 |
| Acc16I | TGCGCA | 1 cut(s) | 2401 |
| Acc36I | ACCTGC | 1 cut(s) | 1831 |
| AccB1I | GGYRCC | 2 cut(s) | 600, 2205 |
| AccB7I | CCANNNNNTGG | 2 cut(s) | 38, 1310 |
| AccI | GTMKAC | 2 cut(s) | 1861, 2088 |
| AciI | CCGC | 4 cut(s) | 215, 731, 777, 2209 |
| AclI | AACGTT | 1 cut(s) | 738 |
| AclWI | GGATC | 6 cut(s) | 60, 89, 433, 453, 2303, 2347 |
| AcoI | YGGCCR | 1 cut(s) | 1654 |
| AcsI | RAATTY | 7 cut(s) | 294, 365, 788, 1063, 1574, 1667, 1803 |
| AcuI | CTGAAG | 4 cut(s) | 340, 1236, 1473, 2240 |
| AcyI | GRCGYC | 2 cut(s) | 153, 601 |
| AdeI | CACNNNGTG | 1 cut(s) | 2963 |
| AfaI | GTAC | 3 cut(s) | 545, 958, 2110 |
| AfiI | CCNNNNNNNGG | 8 cut(s) | 38, 236, 360, 1310, 1742, 2609, 2764, 2864 |
| AhlI | ACTAGT | 2 cut(s) | 959, 1892 |
| AjnI | CCWGG | 3 cut(s) | 612, 1656, 2790 |
| AloI | GAACNNNNNNTCC | 2 cut(s) | 2690, 2722 |
| Alw21I | GWGCWC | 4 cut(s) | 8, 1690, 1779, 2191 |
| Alw26I | GTCTC | 4 cut(s) | 348, 866, 1157, 2942 |
| AlwI | GGATC | 6 cut(s) | 60, 89, 433, 453, 2303, 2347 |
| AlwNI | CAGNNNCTG | 1 cut(s) | 800 |
| Ama87I | CYCGRG | 1 cut(s) | 596 |
| AoxI | GGCC | 5 cut(s) | 1220, 1654, 2690, 2855, 2881 |
| ApeKI | GCWGC | 4 cut(s) | 715, 727, 1934, 2317 |
| ApoI | RAATTY | 7 cut(s) | 294, 365, 788, 1063, 1574, 1667, 1803 |
| AseI | ATTAAT | 1 cut(s) | 1907 |
| Asp700I | GAANNNNTTC | 5 cut(s) | 244, 532, 1750, 1815, 2025 |
| AspA2I | CCTAGG | 1 cut(s) | 1237 |
| AspLEI | GCGC | 4 cut(s) | 603, 1554, 2402, 2970 |
| AspS9I | GGNCC | 2 cut(s) | 655, 1220 |
| AsuC2I | CCSGG | 2 cut(s) | 443, 2866 |
| AsuHPI | GGTGA | 7 cut(s) | 302, 1523, 1739, 2513, 2720, 2949, 3040 |
| AsuII | TTCGAA | 1 cut(s) | 2529 |
| AvaI | CYCGRG | 1 cut(s) | 596 |
| AvaII | GGWCC | 1 cut(s) | 655 |
| AvrII | CCTAGG | 1 cut(s) | 1237 |
| BalI | TGGCCA | 1 cut(s) | 1656 |
| BanI | GGYRCC | 2 cut(s) | 600, 2205 |
| BanII | GRGCYC | 2 cut(s) | 1690, 2191 |
| BarI | GAAGNNNNNNTAC | 8 cut(s) | 643, 675, 1069, 1101, 1234, 1266, 2127, 2159 |
| BauI | CACGAG | 1 cut(s) | 115 |
| BbsI | GAAGAC | 2 cut(s) | 308, 2337 |
| Bbv12I | GWGCWC | 4 cut(s) | 8, 1690, 1779, 2191 |
| BbvI | GCAGC | 4 cut(s) | 702, 714, 1921, 2304 |
| BccI | CCATC | 4 cut(s) | 379, 504, 1456, 2708 |
| BceAI | ACGGC | 1 cut(s) | 1378 |
| BciT130I | CCWGG | 3 cut(s) | 614, 1658, 2792 |
| BciVI | GTATCC | 1 cut(s) | 576 |
| BclI | TGATCA | 1 cut(s) | 405 |
| BcnI | CCSGG | 2 cut(s) | 443, 2866 |
| BcoDI | GTCTC | 4 cut(s) | 348, 866, 1157, 2942 |
| BcuI | ACTAGT | 2 cut(s) | 959, 1892 |
| BfmI | CTRYAG | 4 cut(s) | 139, 234, 485, 2647 |
| BfoI | RGCGCY | 2 cut(s) | 604, 1555 |
| BfuAI | ACCTGC | 1 cut(s) | 1831 |
| BfuI | GTATCC | 1 cut(s) | 576 |
| BisI | GCNGC | 5 cut(s) | 716, 728, 731, 1935, 2318 |
| BlnI | CCTAGG | 1 cut(s) | 1237 |
| BlsI | GCNGC | 5 cut(s) | 717, 729, 732, 1936, 2319 |
| BmcAI | AGTACT | 1 cut(s) | 2110 |
| Bme1390I | CCNGG | 5 cut(s) | 443, 614, 1658, 2792, 2866 |
| Bme18I | GGWCC | 1 cut(s) | 655 |
| BmeT110I | CYCGRG | 1 cut(s) | 596 |
| BmgT120I | GGNCC | 2 cut(s) | 655, 1220 |
| BmiI | GGNNCC | 7 cut(s) | 220, 602, 657, 839, 1662, 2207, 2584 |
| BmrFI | CCNGG | 5 cut(s) | 443, 614, 1658, 2792, 2866 |
| BmrI | ACTGGG | 1 cut(s) | 441 |
| BmsI | GCATC | 4 cut(s) | 818, 1022, 2543, 2916 |
| BmuI | ACTGGG | 1 cut(s) | 441 |
| BpiI | GAAGAC | 2 cut(s) | 308, 2337 |
| Bpu14I | TTCGAA | 1 cut(s) | 2529 |
| BpuEI | CTTGAG | 4 cut(s) | 285, 986, 1676, 2224 |
| BpuMI | CCSGG | 2 cut(s) | 443, 2866 |
| BsaBI | GATNNNNATC | 2 cut(s) | 1998, 3018 |
| BsaHI | GRCGYC | 2 cut(s) | 153, 601 |
| BsaI | GGTCTC | 2 cut(s) | 866, 2942 |
| BsaJI | CCNNGG | 5 cut(s) | 613, 1237, 1528, 2312, 2742 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 822, 852 |
| Bsc4I | CCNNNNNNNGG | 8 cut(s) | 38, 236, 360, 1310, 1742, 2609, 2764, 2864 |
| Bse118I | RCCGGY | 1 cut(s) | 773 |
| Bse1I | ACTGG | 9 cut(s) | 262, 436, 508, 871, 1093, 1441, 1768, 2116, 2731 |
| Bse3DI | GCAATG | 3 cut(s) | 2470, 2783, 2845 |
| Bse8I | GATNNNNATC | 2 cut(s) | 1998, 3018 |
| BseBI | CCWGG | 3 cut(s) | 614, 1658, 2792 |
| BseDI | CCNNGG | 5 cut(s) | 613, 1237, 1528, 2312, 2742 |
| BseGI | GGATG | 4 cut(s) | 1013, 2432, 2624, 2818 |
| BseJI | GATNNNNATC | 2 cut(s) | 1998, 3018 |
| BseLI | CCNNNNNNNGG | 8 cut(s) | 38, 236, 360, 1310, 1742, 2609, 2764, 2864 |
| BseMI | GCAATG | 3 cut(s) | 2470, 2783, 2845 |
| BseMII | CTCAG | 4 cut(s) | 1601, 1607, 1785, 2116 |
| BseNI | ACTGG | 9 cut(s) | 262, 436, 508, 871, 1093, 1441, 1768, 2116, 2731 |
| BseRI | GAGGAG | 1 cut(s) | 1014 |
| BseXI | GCAGC | 4 cut(s) | 702, 714, 1921, 2304 |
| BseYI | CCCAGC | 1 cut(s) | 1035 |
| BsgI | GTGCAG | 3 cut(s) | 373, 1920, 2303 |
| BshFI | GGCC | 5 cut(s) | 1222, 1656, 2692, 2857, 2883 |
| BshNI | GGYRCC | 2 cut(s) | 600, 2205 |
| BsiHKAI | GWGCWC | 4 cut(s) | 8, 1690, 1779, 2191 |
| BsiHKCI | CYCGRG | 1 cut(s) | 596 |
| BsiSI | CCGG | 3 cut(s) | 443, 774, 2865 |
| BslFI | GGGAC | 1 cut(s) | 641 |
| BslI | CCNNNNNNNGG | 8 cut(s) | 38, 236, 360, 1310, 1742, 2609, 2764, 2864 |
| BsmAI | GTCTC | 4 cut(s) | 348, 866, 1157, 2942 |
| BsmBI | CGTCTC | 1 cut(s) | 1157 |
| BsmFI | GGGAC | 1 cut(s) | 641 |
| BsmI | GAATGC | 4 cut(s) | 2186, 2724, 2915, 2970 |
| BsnI | GGCC | 5 cut(s) | 1222, 1656, 2692, 2857, 2883 |
| Bso31I | GGTCTC | 2 cut(s) | 866, 2942 |
| BsoBI | CYCGRG | 1 cut(s) | 596 |
| Bsp119I | TTCGAA | 1 cut(s) | 2529 |
| Bsp1286I | GDGCHC | 4 cut(s) | 8, 1690, 1779, 2191 |
| Bsp1407I | TGTACA | 1 cut(s) | 543 |
| Bsp143I | GATC | 9 cut(s) | 52, 94, 405, 438, 445, 2223, 2308, 2339, 2449 |
| Bsp19I | CCATGG | 1 cut(s) | 2742 |
| BspACI | CCGC | 4 cut(s) | 215, 731, 777, 2209 |
| BspANI | GGCC | 5 cut(s) | 1222, 1656, 2692, 2857, 2883 |
| BspCNI | CTCAG | 4 cut(s) | 1602, 1606, 1784, 2115 |
| BspHI | TCATGA | 1 cut(s) | 2976 |
| BspLI | GGNNCC | 7 cut(s) | 220, 602, 657, 839, 1662, 2207, 2584 |
| BspMAI | CTGCAG | 1 cut(s) | 143 |
| BspMI | ACCTGC | 1 cut(s) | 1831 |
| BspPI | GGATC | 6 cut(s) | 60, 89, 433, 453, 2303, 2347 |
| BspQI | GCTCTTC | 1 cut(s) | 1271 |
| BspT104I | TTCGAA | 1 cut(s) | 2529 |
| BspT107I | GGYRCC | 2 cut(s) | 600, 2205 |
| BspTNI | GGTCTC | 2 cut(s) | 866, 2942 |
| BsrDI | GCAATG | 3 cut(s) | 2470, 2783, 2845 |
| BsrFI | RCCGGY | 1 cut(s) | 773 |
| BsrGI | TGTACA | 1 cut(s) | 543 |
| BsrI | ACTGG | 9 cut(s) | 262, 436, 508, 871, 1093, 1441, 1768, 2116, 2731 |
| BssAI | RCCGGY | 1 cut(s) | 773 |
| BssECI | CCNNGG | 5 cut(s) | 613, 1237, 1528, 2312, 2742 |
| BssMI | GATC | 9 cut(s) | 52, 94, 405, 438, 445, 2223, 2308, 2339, 2449 |
| BssNAI | GTATAC | 1 cut(s) | 2089 |
| BssNI | GRCGYC | 2 cut(s) | 153, 601 |
| BssSI | CACGAG | 1 cut(s) | 115 |
| BssT1I | CCWWGG | 4 cut(s) | 1237, 1528, 2312, 2742 |
| Bst1107I | GTATAC | 1 cut(s) | 2089 |
| Bst2BI | CACGAG | 1 cut(s) | 115 |
| Bst2UI | CCWGG | 3 cut(s) | 614, 1658, 2792 |
| Bst4CI | ACNGT | 4 cut(s) | 548, 802, 2759, 2953 |
| Bst6I | CTCTTC | 4 cut(s) | 756, 1271, 1617, 2531 |
| BstACI | GRCGYC | 2 cut(s) | 153, 601 |
| BstAUI | TGTACA | 1 cut(s) | 543 |
| BstBI | TTCGAA | 1 cut(s) | 2529 |
| BstC8I | GCNNGC | 7 cut(s) | 151, 326, 775, 1556, 2322, 2398, 2518 |
| BstDEI | CTNAG | 8 cut(s) | 398, 980, 1593, 1610, 1771, 2102, 2192, 2720 |
| BstDSI | CCRYGG | 1 cut(s) | 2742 |
| BstEII | GGTNACC | 1 cut(s) | 491 |
| BstENI | CCTNNNNNAGG | 2 cut(s) | 234, 1740 |
| BstF5I | GGATG | 4 cut(s) | 1013, 2432, 2624, 2818 |
| BstH2I | RGCGCY | 2 cut(s) | 604, 1555 |
| BstHHI | GCGC | 4 cut(s) | 603, 1554, 2402, 2970 |
| BstKTI | GATC | 9 cut(s) | 55, 97, 408, 441, 448, 2226, 2311, 2342, 2452 |
| BstMAI | GTCTC | 4 cut(s) | 348, 866, 1157, 2942 |
| BstMBI | GATC | 9 cut(s) | 52, 94, 405, 438, 445, 2223, 2308, 2339, 2449 |
| BstMWI | GCNNNNNNNGC | 5 cut(s) | 159, 609, 724, 990, 1019 |
| BstNI | CCWGG | 3 cut(s) | 614, 1658, 2792 |
| BstNSI | RCATGY | 2 cut(s) | 162, 1830 |
| BstPI | GGTNACC | 1 cut(s) | 491 |
| BstSCI | CCNGG | 5 cut(s) | 441, 612, 1656, 2790, 2864 |
| BstSFI | CTRYAG | 4 cut(s) | 139, 234, 485, 2647 |
| BstV1I | GCAGC | 4 cut(s) | 702, 714, 1921, 2304 |
| BstV2I | GAAGAC | 2 cut(s) | 308, 2337 |
| BstX2I | RGATCY | 3 cut(s) | 94, 2308, 2339 |
| BstXI | CCANNNNNNTGG | 2 cut(s) | 503, 719 |
| BstYI | RGATCY | 3 cut(s) | 94, 2308, 2339 |
| BstZ17I | GTATAC | 1 cut(s) | 2089 |
| BsuI | GTATCC | 1 cut(s) | 576 |
| BsuRI | GGCC | 5 cut(s) | 1222, 1656, 2692, 2857, 2883 |
| BtgI | CCRYGG | 1 cut(s) | 2742 |
| BtsCI | GGATG | 4 cut(s) | 1013, 2432, 2624, 2818 |
| BtsI | GCAGTG | 2 cut(s) | 62, 385 |
| BtsIMutI | CAGTG | 9 cut(s) | 62, 364, 385, 703, 864, 1260, 1585, 1779, 2928 |
| BveI | ACCTGC | 1 cut(s) | 1831 |
| Cac8I | GCNNGC | 7 cut(s) | 151, 326, 775, 1556, 2322, 2398, 2518 |
| CaiI | CAGNNNCTG | 1 cut(s) | 800 |
| CciI | TCATGA | 1 cut(s) | 2976 |
| CfoI | GCGC | 4 cut(s) | 603, 1554, 2402, 2970 |
| Cfr10I | RCCGGY | 1 cut(s) | 773 |
| Cfr13I | GGNCC | 2 cut(s) | 655, 1220 |
| CseI | GACGC | 1 cut(s) | 142 |
| Csp6I | GTAC | 3 cut(s) | 544, 957, 2109 |
| CviQI | GTAC | 3 cut(s) | 544, 957, 2109 |
| DdeI | CTNAG | 8 cut(s) | 398, 980, 1593, 1610, 1771, 2102, 2192, 2720 |
| DinI | GGCGCC | 1 cut(s) | 602 |
| DpnI | GATC | 9 cut(s) | 54, 96, 407, 440, 447, 2225, 2310, 2341, 2451 |
| DpnII | GATC | 9 cut(s) | 52, 94, 405, 438, 445, 2223, 2308, 2339, 2449 |
| DraIII | CACNNNGTG | 1 cut(s) | 2963 |
| EaeI | YGGCCR | 1 cut(s) | 1654 |
| Eam1104I | CTCTTC | 4 cut(s) | 756, 1271, 1617, 2531 |
| EarI | CTCTTC | 4 cut(s) | 756, 1271, 1617, 2531 |
| Ecl136II | GAGCTC | 2 cut(s) | 1688, 2189 |
| Eco130I | CCWWGG | 4 cut(s) | 1237, 1528, 2312, 2742 |
| Eco147I | AGGCCT | 1 cut(s) | 2883 |
| Eco24I | GRGCYC | 2 cut(s) | 1690, 2191 |
| Eco31I | GGTCTC | 2 cut(s) | 866, 2942 |
| Eco32I | GATATC | 1 cut(s) | 2644 |
| Eco47I | GGWCC | 1 cut(s) | 655 |
| Eco53kI | GAGCTC | 2 cut(s) | 1688, 2189 |
| Eco57I | CTGAAG | 4 cut(s) | 340, 1236, 1473, 2240 |
| Eco88I | CYCGRG | 1 cut(s) | 596 |
| Eco91I | GGTNACC | 1 cut(s) | 491 |
| EcoICRI | GAGCTC | 2 cut(s) | 1688, 2189 |
| EcoNI | CCTNNNNNAGG | 2 cut(s) | 234, 1740 |
| EcoO65I | GGTNACC | 1 cut(s) | 491 |
| EcoRI | GAATTC | 2 cut(s) | 1574, 1803 |
| EcoRII | CCWGG | 3 cut(s) | 612, 1656, 2790 |
| EcoRV | GATATC | 1 cut(s) | 2644 |
| EcoT14I | CCWWGG | 4 cut(s) | 1237, 1528, 2312, 2742 |
| EcoT38I | GRGCYC | 2 cut(s) | 1690, 2191 |
| EgeI | GGCGCC | 1 cut(s) | 602 |
| EheI | GGCGCC | 1 cut(s) | 602 |
| ErhI | CCWWGG | 4 cut(s) | 1237, 1528, 2312, 2742 |
| Esp3I | CGTCTC | 1 cut(s) | 1157 |
| FalI | AAGNNNNNCTT | 2 cut(s) | 1996, 2028 |
| FaqI | GGGAC | 1 cut(s) | 641 |
| FauI | CCCGC | 1 cut(s) | 208 |
| FbaI | TGATCA | 1 cut(s) | 405 |
| FblI | GTMKAC | 2 cut(s) | 1861, 2088 |
| Fnu4HI | GCNGC | 5 cut(s) | 716, 728, 731, 1935, 2318 |
| FokI | GGATG | 4 cut(s) | 1000, 2419, 2631, 2825 |
| FriOI | GRGCYC | 2 cut(s) | 1690, 2191 |
| Fsp4HI | GCNGC | 5 cut(s) | 716, 728, 731, 1935, 2318 |
| FspI | TGCGCA | 1 cut(s) | 2401 |
| GlaI | GCGC | 4 cut(s) | 602, 1553, 2401, 2969 |
| GluI | GCNGC | 5 cut(s) | 716, 728, 731, 1935, 2318 |
| GsaI | CCCAGC | 1 cut(s) | 1039 |
| HaeII | RGCGCY | 2 cut(s) | 604, 1555 |
| HaeIII | GGCC | 5 cut(s) | 1222, 1656, 2692, 2857, 2883 |
| HapII | CCGG | 3 cut(s) | 443, 774, 2865 |
| HgaI | GACGC | 1 cut(s) | 142 |
| HhaI | GCGC | 4 cut(s) | 603, 1554, 2402, 2970 |
| Hin1I | GRCGYC | 2 cut(s) | 153, 601 |
| Hin6I | GCGC | 4 cut(s) | 601, 1552, 2400, 2968 |
| HinP1I | GCGC | 4 cut(s) | 601, 1552, 2400, 2968 |
| HincII | GTYRAC | 4 cut(s) | 471, 784, 2034, 2776 |
| HindII | GTYRAC | 4 cut(s) | 471, 784, 2034, 2776 |
| HindIII | AAGCTT | 3 cut(s) | 1172, 2074, 2518 |
| HpaI | GTTAAC | 1 cut(s) | 471 |
| HpaII | CCGG | 3 cut(s) | 443, 774, 2865 |
| HphI | GGTGA | 7 cut(s) | 302, 1523, 1739, 2513, 2720, 2949, 3040 |
| Hpy188I | TCNGA | 7 cut(s) | 1206, 1453, 1522, 1781, 1974, 2220, 2559 |
| Hpy99I | CGWCG | 1 cut(s) | 2339 |
| HpyCH4III | ACNGT | 4 cut(s) | 548, 802, 2759, 2953 |
| HpyCH4IV | ACGT | 2 cut(s) | 738, 1939 |
| HpyF10VI | GCNNNNNNNGC | 5 cut(s) | 159, 609, 724, 990, 1019 |
| HpyF3I | CTNAG | 8 cut(s) | 398, 980, 1593, 1610, 1771, 2102, 2192, 2720 |
| HpySE526I | ACGT | 2 cut(s) | 738, 1939 |
| Hsp92I | GRCGYC | 2 cut(s) | 153, 601 |
| HspAI | GCGC | 4 cut(s) | 601, 1552, 2400, 2968 |
| KasI | GGCGCC | 1 cut(s) | 600 |
| KroI | GCCGGC | 1 cut(s) | 773 |
| KroNI | GCCGGC | 1 cut(s) | 775 |
| Ksp22I | TGATCA | 1 cut(s) | 405 |
| KspAI | GTTAAC | 1 cut(s) | 471 |
| Kzo9I | GATC | 9 cut(s) | 52, 94, 405, 438, 445, 2223, 2308, 2339, 2449 |
| LguI | GCTCTTC | 1 cut(s) | 1271 |
| LmnI | GCTCC | 5 cut(s) | 843, 1549, 2156, 2793, 2951 |
| Lsp1109I | GCAGC | 4 cut(s) | 702, 714, 1921, 2304 |
| LweI | GCATC | 4 cut(s) | 818, 1022, 2543, 2916 |
| MaeII | ACGT | 2 cut(s) | 738, 1939 |
| MaeIII | GTNAC | 9 cut(s) | 154, 491, 700, 1241, 1255, 1349, 2258, 2501, 3046 |
| MalI | GATC | 9 cut(s) | 54, 96, 407, 440, 447, 2225, 2310, 2341, 2451 |
| MboI | GATC | 9 cut(s) | 52, 94, 405, 438, 445, 2223, 2308, 2339, 2449 |
| MfeI | CAATTG | 1 cut(s) | 1899 |
| MflI | RGATCY | 3 cut(s) | 94, 2308, 2339 |
| MhlI | GDGCHC | 4 cut(s) | 8, 1690, 1779, 2191 |
| MlsI | TGGCCA | 1 cut(s) | 1656 |
| MluNI | TGGCCA | 1 cut(s) | 1656 |
| Mly113I | GGCGCC | 1 cut(s) | 601 |
| MlyI | GAGTC | 1 cut(s) | 189 |
| MmeI | TCCRAC | 1 cut(s) | 1608 |
| Mox20I | TGGCCA | 1 cut(s) | 1656 |
| MroNI | GCCGGC | 1 cut(s) | 773 |
| MroXI | GAANNNNTTC | 5 cut(s) | 244, 532, 1750, 1815, 2025 |
| MscI | TGGCCA | 1 cut(s) | 1656 |
| MseI | TTAA | 7 cut(s) | 75, 470, 908, 1392, 1907, 2130, 2138 |
| MslI | CAYNNNNRTG | 4 cut(s) | 2499, 2741, 2975, 2981 |
| Msp20I | TGGCCA | 1 cut(s) | 1656 |
| MspA1I | CMGCKG | 1 cut(s) | 715 |
| MspI | CCGG | 3 cut(s) | 443, 774, 2865 |
| MspR9I | CCNGG | 5 cut(s) | 443, 614, 1658, 2792, 2866 |
| MunI | CAATTG | 1 cut(s) | 1899 |
| Mva1269I | GAATGC | 4 cut(s) | 2186, 2724, 2915, 2970 |
| MvaI | CCWGG | 3 cut(s) | 614, 1658, 2792 |
| MwoI | GCNNNNNNNGC | 5 cut(s) | 159, 609, 724, 990, 1019 |
| NaeI | GCCGGC | 1 cut(s) | 775 |
| NarI | GGCGCC | 1 cut(s) | 601 |
| NciI | CCSGG | 2 cut(s) | 443, 2866 |
| NcoI | CCATGG | 1 cut(s) | 2742 |
| NdeII | GATC | 9 cut(s) | 52, 94, 405, 438, 445, 2223, 2308, 2339, 2449 |
| NgoMIV | GCCGGC | 1 cut(s) | 773 |
| NlaIV | GGNNCC | 7 cut(s) | 220, 602, 657, 839, 1662, 2207, 2584 |
| NmuCI | GTSAC | 4 cut(s) | 154, 1255, 2501, 3046 |
| NsbI | TGCGCA | 1 cut(s) | 2401 |
| NspI | RCATGY | 2 cut(s) | 162, 1830 |
| NspV | TTCGAA | 1 cut(s) | 2529 |
| PagI | TCATGA | 1 cut(s) | 2976 |
| PceI | AGGCCT | 1 cut(s) | 2883 |
| PciSI | GCTCTTC | 1 cut(s) | 1271 |
| PctI | GAATGC | 4 cut(s) | 2186, 2724, 2915, 2970 |
| PdiI | GCCGGC | 1 cut(s) | 775 |
| PdmI | GAANNNNTTC | 5 cut(s) | 244, 532, 1750, 1815, 2025 |
| PflFI | GACNNNGTC | 3 cut(s) | 1417, 2563, 2759 |
| PflMI | CCANNNNNTGG | 2 cut(s) | 38, 1310 |
| PfoI | TCCNGGA | 2 cut(s) | 441, 2790 |
| PkrI | GCNGC | 5 cut(s) | 717, 729, 732, 1936, 2319 |
| PleI | GAGTC | 1 cut(s) | 188 |
| PluTI | GGCGCC | 1 cut(s) | 604 |
| PpsI | GAGTC | 1 cut(s) | 188 |
| PshBI | ATTAAT | 1 cut(s) | 1907 |
| PsiI | TTATAA | 1 cut(s) | 672 |
| Psp124BI | GAGCTC | 2 cut(s) | 1690, 2191 |
| Psp1406I | AACGTT | 1 cut(s) | 738 |
| Psp6I | CCWGG | 3 cut(s) | 612, 1656, 2790 |
| PspEI | GGTNACC | 1 cut(s) | 491 |
| PspFI | CCCAGC | 1 cut(s) | 1035 |
| PspGI | CCWGG | 3 cut(s) | 612, 1656, 2790 |
| PspN4I | GGNNCC | 7 cut(s) | 220, 602, 657, 839, 1662, 2207, 2584 |
| PspPI | GGNCC | 2 cut(s) | 655, 1220 |
| PstI | CTGCAG | 1 cut(s) | 143 |
| PstNI | CAGNNNCTG | 1 cut(s) | 800 |
| PsuI | RGATCY | 3 cut(s) | 94, 2308, 2339 |
| PsyI | GACNNNGTC | 3 cut(s) | 1417, 2563, 2759 |
| PvuII | CAGCTG | 1 cut(s) | 715 |
| RsaI | GTAC | 3 cut(s) | 545, 958, 2110 |
| RsaNI | GTAC | 3 cut(s) | 544, 957, 2109 |
| RseI | CAYNNNNRTG | 4 cut(s) | 2499, 2741, 2975, 2981 |
| SacI | GAGCTC | 2 cut(s) | 1690, 2191 |
| SapI | GCTCTTC | 1 cut(s) | 1271 |
| SaqAI | TTAA | 7 cut(s) | 75, 470, 908, 1392, 1907, 2130, 2138 |
| SatI | GCNGC | 5 cut(s) | 716, 728, 731, 1935, 2318 |
| Sau3AI | GATC | 9 cut(s) | 52, 94, 405, 438, 445, 2223, 2308, 2339, 2449 |
| Sau96I | GGNCC | 2 cut(s) | 655, 1220 |
| ScaI | AGTACT | 1 cut(s) | 2110 |
| SchI | GAGTC | 1 cut(s) | 189 |
| ScrFI | CCNGG | 5 cut(s) | 443, 614, 1658, 2792, 2866 |
| SduI | GDGCHC | 4 cut(s) | 8, 1690, 1779, 2191 |
| SfaNI | GCATC | 4 cut(s) | 818, 1022, 2543, 2916 |
| SfcI | CTRYAG | 4 cut(s) | 139, 234, 485, 2647 |
| SfoI | GGCGCC | 1 cut(s) | 602 |
| SfuI | TTCGAA | 1 cut(s) | 2529 |
| SinI | GGWCC | 1 cut(s) | 655 |
| SmiMI | CAYNNNNRTG | 4 cut(s) | 2499, 2741, 2975, 2981 |
| SmlI | CTYRAG | 4 cut(s) | 300, 965, 1691, 2239 |
| SmoI | CTYRAG | 4 cut(s) | 300, 965, 1691, 2239 |
| SpeI | ACTAGT | 2 cut(s) | 959, 1892 |
| SseBI | AGGCCT | 1 cut(s) | 2883 |
| SsiI | CCGC | 4 cut(s) | 215, 731, 777, 2209 |
| SspDI | GGCGCC | 1 cut(s) | 600 |
| SspI | AATATT | 2 cut(s) | 676, 2380 |
| SstI | GAGCTC | 2 cut(s) | 1690, 2191 |
| StuI | AGGCCT | 1 cut(s) | 2883 |
| StyD4I | CCNGG | 5 cut(s) | 441, 612, 1656, 2790, 2864 |
| StyI | CCWWGG | 4 cut(s) | 1237, 1528, 2312, 2742 |
| TaaI | ACNGT | 4 cut(s) | 548, 802, 2759, 2953 |
| TaiI | ACGT | 2 cut(s) | 741, 1942 |
| TaqI | TCGA | 7 cut(s) | 437, 632, 1403, 1684, 1853, 2475, 2529 |
| TatI | WGTACW | 3 cut(s) | 543, 956, 2108 |
| TauI | GCSGC | 1 cut(s) | 733 |
| Tru1I | TTAA | 7 cut(s) | 75, 470, 908, 1392, 1907, 2130, 2138 |
| Tru9I | TTAA | 7 cut(s) | 75, 470, 908, 1392, 1907, 2130, 2138 |
| TscAI | CASTG | 9 cut(s) | 69, 364, 392, 703, 871, 1260, 1585, 1779, 2928 |
| TseFI | GTSAC | 4 cut(s) | 154, 1255, 2501, 3046 |
| TseI | GCWGC | 4 cut(s) | 715, 727, 1934, 2317 |
| Tsp45I | GTSAC | 4 cut(s) | 154, 1255, 2501, 3046 |
| TspGWI | ACGGA | 2 cut(s) | 2352, 2711 |
| TspRI | CASTG | 9 cut(s) | 69, 364, 392, 703, 871, 1260, 1585, 1779, 2928 |
| Tth111I | GACNNNGTC | 3 cut(s) | 1417, 2563, 2759 |
| Van91I | CCANNNNNTGG | 2 cut(s) | 38, 1310 |
| VpaK11BI | GGWCC | 1 cut(s) | 655 |
| VspI | ATTAAT | 1 cut(s) | 1907 |
| XagI | CCTNNNNNAGG | 2 cut(s) | 234, 1740 |
| XapI | RAATTY | 7 cut(s) | 294, 365, 788, 1063, 1574, 1667, 1803 |
| XbaI | TCTAGA | 3 cut(s) | 13, 827, 2305 |
| XceI | RCATGY | 2 cut(s) | 162, 1830 |
| XmaJI | CCTAGG | 1 cut(s) | 1237 |
| XmiI | GTMKAC | 2 cut(s) | 1861, 2088 |
| XmnI | GAANNNNTTC | 5 cut(s) | 244, 532, 1750, 1815, 2025 |
| ZrmI | AGTACT | 1 cut(s) | 2110 |