MD12G1164300.v1.1

LRR receptor-like serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr12
OG0000262
Physical Location & Seq
Forward (+)
24567558 .. 24570882
3325 bp
Loading structure...
UTR
Exon/CDS
Intron
MD12G1164300.v1.1.491

Sequence Viewer

Length: 3126 bp
ATGGAGCCTTCGTATACTTACTGTACGAATTTTTTGTTTAAATTCCTTCCCGGGTTCTTTCTCTTATGCATGATCTCGTGCCTGGAATCCACATTCGGAAACAAATCTGATCGTCTCGCTCTGCTGGACTTCAAGAGGAGAATAACAGAAGATCCTCTCCATATCATGAACTCATGGAATGATTCGGTCGATTTGTGCAGTTGGGTTGGCGTTACGTGCAACCATGCGAACAAAAGAGTCATGATCTTGAACTTGGAAGCTCAAAAGTTGGTAGGCCCCGTACCGCCTTCAATCGGAAATCTTACTTATCTTAATGGAATCAACTTGATAGACAACAACTTTCACGGCGAAATTCCTCAAGAAATCGGACGTCTACTAGGCTTGCAGTATCTCAACCTGACTTCGAATTCTTTCGGCGGGAAAATTCCAGCTAATATATCTCACTGCACGCAGCTAAGAATGCTCAATGTTGGTTCAAATAAGCTGATTGGGTCAATTCCGAACCAACTCAATTCGTTGTTGAATTTAGCCCATTTTTTGGTTGGTAAAAACAATCTCACCGGAACAATTCCGGATTGGATAGGAAACTTTTCTTCCATGTCTGCAATCTCTCTTGATAACAACAATTTTGAAGGAAGCATACCATATGCGCTCGGGCGGTTGACAAGCTTGGGAAGCTTCGAACTTGCATCAAACAAACTTTCCGGTACGGTTCCTTCTTCAATCTATAATATATCGTCGATATACTCAATTCATGTTACCAACAACCAGCTGCATGGAGAGCTCCCACAAAATGTGGGGACTACTCTTCCTAATCTTGAGTTATTTGCTGGCGGTGTCAACAAATTCACCGGAAGTATTCCTGTTTCTTTGTCAAATGCTTCTAAACTTCGATTTCTTGATTTTGCCGAAAATGGCCTCACGGGAAAATTCCCTGCTGAAAATTTTGGAACTTTGAAAGGGTTGGTGAGACTAAACTTTGATGCCAATAGGCTGGGAAGTGGGAAAACAGGTGACCTGCGTTCTCTCAGTTTTCTGGCCAATTGTACTGATCTTGAGGTGTTAAGTTTTAGTCGTAATCGTTTTGGAGGGGAATTGCCAGAGTCCATAGGCAACCTTTCGACAAAACTGAGAATTTTTACAATGGGGGGAAATTTATTACATGGAAGCTTCCCTACCCGGGTTGTGAATCTTGTAAACTTGATCAATCTAGGAATGGAACAAAACTATCTTGGTGGTAGTGTCCCTGATGTGATCGGTAAGCTTCAGAAGTTGCACGGGCTGTATTTGAATCTTAACCAATTCTCTGGGACAATCCCGTCCACGCTAGGCAACTTGACTTCAGTCACAAGGCTCTTCATGGAGGGGAATAGGTTTGAGGGAAGCATATCTCCGAGTCTCGGGAACTGCCAAAGCTTATTGATGCTTAACCTTTCTAGTAATCAGCTAAGTGGCACCATACCTAAAGAGGTTATCGGGCTTTCATCTCTATCAATTTCTTTGTCCATGTCGAACAATTCTTTGACTGGTTCTCTACCATCTGAAGTGGGCGAGTTGGTACATCTCTCGGAGCTAGATGTATCAGGAAACAAGTTATCGGGTGAAATCCCCATAACCCTCGGCAGCTGTACTAGTTTGGTGAGCCTACATTTGGAAGGTAACCAATTTGAAGGAACAATTCCTCAATCTCTAGCCAACTTAAGAGGCTTGGAAGAAATAGATATTTCACGCAATAATTTATCTGGCCAGATTCCTGAATGTTTAGGTAAGCTTGGAGCTCTAAAGCAACTCAATATTTCCTATAATGAATTTGAGGGTGAATTGCCCACAGAAGGGATATTTTCAAATGCGAGTGGTTTCTCGGTTCTTGGAAATAATAAGCTCTGCGGTGGCATCCGAGAATTACGTCTACATGTATGCACCAGCAAAAAATCTCATTCATCTCAAGGACTACTTGCCCCGAAAGTAATAATCCCTCTAACTTGTGCACTTGCATTAATAATTACTCTGTCATGTTTCATTGCTTCTTGTGCATTGGTGAAAAGGTCAAGAGGTAGACCTTTAACTTCACGTTCTAATGAAGAATCAAAATCAGGTGTCTCCTATTTACAACTCGTTGAATCAACCAATGGGTTCTCTCCAGAAAATCTCATTGGTTCGGGAAGTTTTGGTTCTGTATACAGGGGAGTACTCTCTGGTAATGGCATCATAGTTGCCGTTAAGGTGTTAAACCTTCAACAAGAAGGAGCTTCCAAGAGTTTCATTGATGAATGCAAAGCTTTAAGAAGTGTAAGGCACCGCAATCTTCTCAAGATCATAACTGCATGCTCGAGCATTGATAGTCAAGGTAATGAGTTCAAAAGTCTAGTTTATGAGTTCATGGAAAATGGAAGCCTGGACCCTTGGCTGCATCCAAGGGATGACGAAGAATCTCCAAACAAGAGATTGAGCCTTACCCAAAGACTGAATATTGCCATTGATGTCGCTTCTGTACTAGATTATCTACACCACAATTGTGAAACATGCATTGTTCATTGTGATTTAAAGCCAAGCAATATTCTTCTTGATGAAGATATGGTTGCTCACGTTGGTGACTTTGGCCTAGCAAAGTTCCTCTTGGAGGCATCAAATGGTACAACCTTGTCAGTAGGGCTAAAAGGTTCCACAGGCTACATTCCTCCAGAGTATGGGATGGGAGGACAAGTCTCCACACAAGGAGATGTTTATAGTTACGGGATACTGTTGCTAGAAATGTTCACAGGAAAAAGGCCAACCGATGACATTTTCAAAGATGGTCTAAGCATTCACCAATTCACCGCCATGGCATTGCCGGACCATGTCAAGGAAGTAGTTGAGCCCTCATTGCTCCTCGAAACAGATGACGAAGATGTTGAAGATGACGATGGCGACGGATATGGGAATAAAATACGGGAAAGACCACTGGCCAAATACAAGGATCCCGGCCCGGACAAAGCAAAAAGACGGGAGGAATGCTTGTATTCGGTGATACAAATAGGCATCTCGTGCTCCGCAATATCACCAGGAGAGCGGATGCGTATGGATGTGGTTGTCAACAAAATGGGTGCACTTAGAGACTCATATCTCAACTTAAGAAGAAGAAGATGA

Protein Analysis

1042

Amino Acids

113.1

Weight (kDa)

6.07

Isoelectric Point (pI)

37.95

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRRNT_2 PF08263 35 - 74 1.4e-11 Leucine rich repeat N-terminal domain
LRR_14 PF23598 94 - 179 1.6e-06 Leucine-rich repeat region
LRR_14 PF23598 120 - 301 1.5e-09 Leucine-rich repeat region
LRR_14 PF23598 341 - 476 6.2e-07 Leucine-rich repeat region
LRR_14 PF23598 462 - 604 8.3e-13 Leucine-rich repeat region
LRR_8 PF13855 545 - 603 7.3e-09 Leucine rich repeat
Pkinase PF00069 715 - 931 9.2e-40 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 716 - 928 1.9e-39 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000262)

Species Orthologous Gene IDs
fragaria_vesca FvH4_6g11680 FvH4_6g11680 FvH4_6g11680 FvH4_6g11680 FvH4_6g11680 FvH4_6g11680 FvH4_6g11681 FvH4_6g11682 FvH4_6g11683
malus_domestica MD00G1079100.v1.1 MD00G1080000.v1.1 MD01G1100900.v1.1 MD03G1225200.v1.1 MD04G1041600.v1.1 MD04G1110500.v1.1 MD04G1118700.v1.1 MD04G1150300.v1.1 MD04G1150400.v1.1 MD04G1156000.v1.1 MD04G1156200.v1.1 MD04G1156700.v1.1 MD04G1157400.v1.1 MD04G1157500.v1.1 MD04G1157700.v1.1 MD04G1158000.v1.1 MD04G1158200.v1.1 MD04G1158600.v1.1 MD04G1159000.v1.1 MD04G1159100.v1.1 MD05G1061600.v1.1 MD05G1075800.v1.1 MD06G1129300.v1.1 MD07G1217800.v1.1 MD07G1218000.v1.1 MD07G1308700.v1.1 MD12G1130700.v1.1 MD12G1130800.v1.1 MD12G1134500.v1.1 MD12G1134700.v1.1 MD12G1135000.v1.1 MD12G1135200.v1.1 MD12G1135800.v1.1 MD12G1164300.v1.1 MD12G1164400.v1.1 MD12G1169100.v1.1 MD12G1169700.v1.1 MD12G1170200.v1.1 MD12G1170400.v1.1 MD13G1275300.v1.1
prunus_persica Prupe.1G072900_v2.0.a1 Prupe.1G194200_v2.0.a1 Prupe.1G201100_v2.0.a1 Prupe.6G248000_v2.0.a1 Prupe.6G248100_v2.0.a1 Prupe.6G248500_v2.0.a1 Prupe.6G274800_v2.0.a1 Prupe.6G274900_v2.0.a1 Prupe.6G275100_v2.0.a1 Prupe.6G275100_v2.0.a1 Prupe.7G044200_v2.0.a1 Prupe.7G044300_v2.0.a1 Prupe.7G044700_v2.0.a1
pyrus_communis pycom04g03530 pycom04g10490 pycom04g10500 pycom04g10510 pycom04g10560 pycom04g13580 pycom04g13600 pycom04g14080 pycom12g10580 pycom12g11020 pycom12g12650 pycom12g12680 pycom12g15730 pycom12g15740 pycom12g16060 pycom12g16100 pycom12g16200 pycom12g16240 pycom16g21060
rosa_chinensis RchiOBHm_Chr3g0463401 RchiOBHm_Chr3g0463411 RchiOBHm_Chr3g0463481 RchiOBHm_Chr4g0408571 RchiOBHm_Chr4g0408641
rosa_laevigata RLG00000019546 RLG00000019547 RLG00000019552 RLG00000019553 RLG00000019554 RLG00000019578 RLG00000024784 RLG00000024785 RLG00000024786
rosa_multiflora Rmu_sc0000923.1_g000013 Rmu_sc0000923.1_g000017 Rmu_sc0000923.1_g000018 Rmu_sc0001053.1_g000005 Rmu_sc0001053.1_g000012 Rmu_sc0001053.1_g000013 Rmu_sc0001053.1_g000014 Rmu_sc0031971.1_g000001
rosa_roxburghii Rroxscaffold_2G00107980 Rroxscaffold_6G00416540 Rroxscaffold_6G00416560
rosa_rugosa Rorug02G0333800 Rorug02G0333900 Rorug03G0064500 Rorug03G0064600 Rorug03G0064800 Rorug03G0064900 Rorug03G0065000 Rorug03G0065000
rosa_samantha Rh2CG370900 Rh3AG122100 Rh3BG126500 Rh3BG126800 Rh3BG126900 Rh3CG128600 Rh3CG128700 Rh3DG127400 Rh3DG127500 Rh3DG127700 Rh4CG158500 Rh4CG158600
rosa_wichuraiana Rw0G010610 Rw0G010620 Rw3G010370 Rw3G010380 Rw3G010400 Rw3G010410

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 1318
AatII GACGTC 1 cut(s) 373
Acc36I ACCTGC 1 cut(s) 1026
AccB1I GGYRCC 2 cut(s) 1454, 2295
AccB7I CCANNNNNTGG 2 cut(s) 538, 2687
AccBSI CCGCTC 1 cut(s) 3049
AccI GTMKAC 5 cut(s) 14, 373, 1909, 2056, 2178
AccIII TCCGGA 1 cut(s) 571
AciI CCGC 9 cut(s) 284, 417, 658, 834, 1887, 2299, 2817, 3030, 3049
AclWI GGATC 3 cut(s) 146, 2951, 2964
AcoI YGGCCR 3 cut(s) 1038, 1744, 2943
AcuI CTGAAG 3 cut(s) 1250, 1326, 1563
AcyI GRCGYC 1 cut(s) 370
AfaI GTAC 9 cut(s) 25, 282, 709, 1048, 1560, 1630, 2190, 2493, 2635
AflII CTTAAG 2 cut(s) 1699, 3109
AflIII ACRYGT 1 cut(s) 1912
AhlI ACTAGT 1 cut(s) 1631
AjnI CCWGG 3 cut(s) 81, 2394, 3040
AjuI GAANNNNNNNTTGG 2 cut(s) 2136, 2168
AleI CACNNNNGTG 2 cut(s) 2514, 2589
Alw21I GWGCWC 5 cut(s) 786, 1780, 1990, 3029, 3088
Alw26I GTCTC 6 cut(s) 119, 964, 1403, 2104, 2710, 3087
Alw44I GTGCAC 2 cut(s) 1986, 3084
AlwI GGATC 3 cut(s) 146, 2951, 2964
Ama87I CYCGRG 5 cut(s) 50, 653, 1179, 1400, 2329
Aor13HI TCCGGA 1 cut(s) 571
AoxI GGCC 8 cut(s) 274, 916, 1038, 1744, 2599, 2768, 2943, 2962
ApaLI GTGCAC 2 cut(s) 1986, 3084
ApeKI GCWGC 4 cut(s) 451, 772, 1623, 2407
AseI ATTAAT 1 cut(s) 1997
Asp700I GAANNNNTTC 2 cut(s) 410, 589
AspLEI GCGC 1 cut(s) 652
AspS9I GGNCC 4 cut(s) 275, 2398, 2833, 2963
AsuC2I CCSGG 6 cut(s) 51, 52, 1180, 1181, 2961, 2966
AsuII TTCGAA 2 cut(s) 404, 681
AvaI CYCGRG 5 cut(s) 50, 653, 1179, 1400, 2329
AvaII GGWCC 2 cut(s) 2398, 2833
BaeGI GKGCMC 2 cut(s) 1990, 3088
BalI TGGCCA 3 cut(s) 1040, 1746, 2945
BamHI GGATCC 1 cut(s) 2956
BanI GGYRCC 2 cut(s) 1454, 2295
BanII GRGCYC 3 cut(s) 786, 1780, 2859
BarI GAAGNNNNNNTAC 6 cut(s) 624, 656, 700, 732, 1159, 1191
BauI CACGAG 2 cut(s) 76, 3022
Bbv12I GWGCWC 5 cut(s) 786, 1780, 1990, 3029, 3088
BbvI GCAGC 4 cut(s) 463, 759, 1635, 2394
BccI CCATC 4 cut(s) 1546, 2686, 2786, 2897
BceAI ACGGC 2 cut(s) 361, 2201
BciT130I CCWGG 3 cut(s) 83, 2396, 3042
BciVI GTATCC 1 cut(s) 2730
BclI TGATCA 1 cut(s) 1203
BcnI CCSGG 6 cut(s) 51, 52, 1180, 1181, 2961, 2966
BcoDI GTCTC 6 cut(s) 119, 964, 1403, 2104, 2710, 3087
BcuI ACTAGT 1 cut(s) 1631
BfrI CTTAAG 2 cut(s) 1699, 3109
BfuAI ACCTGC 1 cut(s) 1026
BfuI GTATCC 1 cut(s) 2730
BisI GCNGC 4 cut(s) 452, 773, 1624, 2408
BlsI GCNGC 4 cut(s) 453, 774, 1625, 2409
BmcAI AGTACT 1 cut(s) 2190
Bme1390I CCNGG 9 cut(s) 51, 52, 83, 1180, 1181, 2396, 2961, 2966, 3042
Bme18I GGWCC 2 cut(s) 2398, 2833
BmeT110I CYCGRG 5 cut(s) 50, 653, 1179, 1400, 2329
BmgT120I GGNCC 4 cut(s) 275, 2398, 2833, 2963
BmiI GGNNCC 8 cut(s) 6, 277, 714, 1456, 2297, 2400, 2662, 2958
BmrFI CCNGG 9 cut(s) 51, 52, 83, 1180, 1181, 2396, 2961, 2966, 3042
BmsI GCATC 9 cut(s) 698, 973, 1413, 1902, 2214, 2419, 2633, 3027, 3042
BoxI GACNNNNGTC 1 cut(s) 1343
BpmI CTGGAG 2 cut(s) 2124, 2664
Bpu14I TTCGAA 2 cut(s) 404, 681
BpuEI CTTGAG 5 cut(s) 342, 839, 1076, 1929, 2294
BpuMI CCSGG 6 cut(s) 51, 52, 1180, 1181, 2961, 2966
BsaAI YACGTR 1 cut(s) 216
BsaHI GRCGYC 1 cut(s) 370
BsaJI CCNNGG 6 cut(s) 50, 1179, 1618, 2402, 2414, 2820
BsaWI WCCGGW 4 cut(s) 560, 571, 704, 851
BsaXI ACNNNNNCTCC 3 cut(s) 26, 2688, 2718
Bse1I ACTGG 2 cut(s) 1531, 2946
Bse3DI GCAATG 3 cut(s) 2019, 2825, 2861
BseAI TCCGGA 1 cut(s) 571
BseBI CCWGG 3 cut(s) 83, 2396, 3042
BseDI CCNNGG 6 cut(s) 50, 1179, 1618, 2402, 2414, 2820
BseGI GGATG 6 cut(s) 1893, 2410, 2425, 2697, 3057, 3067
BseMI GCAATG 3 cut(s) 2019, 2825, 2861
BseMII CTCAG 2 cut(s) 1042, 1121
BseNI ACTGG 2 cut(s) 1531, 2946
BseRI GAGGAG 2 cut(s) 151, 2858
BseSI GKGCMC 2 cut(s) 1990, 3088
BseXI GCAGC 4 cut(s) 463, 759, 1635, 2394
BseYI CCCAGC 1 cut(s) 994
BsgI GTGCAG 2 cut(s) 217, 430
Bsh1285I CGRYCG 1 cut(s) 189
BshFI GGCC 8 cut(s) 276, 918, 1040, 1746, 2601, 2770, 2945, 2964
BshNI GGYRCC 2 cut(s) 1454, 2295
BsiEI CGRYCG 1 cut(s) 189
BsiHKAI GWGCWC 5 cut(s) 786, 1780, 1990, 3029, 3088
BsiHKCI CYCGRG 5 cut(s) 50, 653, 1179, 1400, 2329
BsiSI CCGG 9 cut(s) 51, 561, 572, 705, 852, 1180, 2831, 2961, 2966
BslFI GGGAC 3 cut(s) 814, 1229, 1324
BsmAI GTCTC 6 cut(s) 119, 964, 1403, 2104, 2710, 3087
BsmBI CGTCTC 1 cut(s) 119
BsmFI GGGAC 3 cut(s) 814, 1229, 1324
BsmI GAATGC 4 cut(s) 465, 2276, 2802, 2996
BsnI GGCC 8 cut(s) 276, 918, 1040, 1746, 2601, 2770, 2945, 2964
BsoBI CYCGRG 5 cut(s) 50, 653, 1179, 1400, 2329
Bsp119I TTCGAA 2 cut(s) 404, 681
Bsp1286I GDGCHC 6 cut(s) 786, 1780, 1990, 2859, 3029, 3088
Bsp13I TCCGGA 1 cut(s) 571
Bsp143I GATC 9 cut(s) 72, 109, 151, 243, 1051, 1203, 1254, 2313, 2956
Bsp19I CCATGG 1 cut(s) 2820
BspACI CCGC 9 cut(s) 284, 417, 658, 834, 1887, 2299, 2817, 3030, 3049
BspANI GGCC 8 cut(s) 276, 918, 1040, 1746, 2601, 2770, 2945, 2964
BspCNI CTCAG 2 cut(s) 1041, 1122
BspEI TCCGGA 1 cut(s) 571
BspHI TCATGA 2 cut(s) 165, 240
BspLI GGNNCC 8 cut(s) 6, 277, 714, 1456, 2297, 2400, 2662, 2958
BspMI ACCTGC 1 cut(s) 1026
BspPI GGATC 3 cut(s) 146, 2951, 2964
BspQI GCTCTTC 1 cut(s) 1361
BspT104I TTCGAA 2 cut(s) 404, 681
BspT107I GGYRCC 2 cut(s) 1454, 2295
BspTI CTTAAG 2 cut(s) 1699, 3109
BsrBI CCGCTC 1 cut(s) 3049
BsrDI GCAATG 3 cut(s) 2019, 2825, 2861
BsrI ACTGG 2 cut(s) 1531, 2946
BssECI CCNNGG 6 cut(s) 50, 1179, 1618, 2402, 2414, 2820
BssMI GATC 9 cut(s) 72, 109, 151, 243, 1051, 1203, 1254, 2313, 2956
BssNAI GTATAC 2 cut(s) 15, 2179
BssNI GRCGYC 1 cut(s) 370
BssSI CACGAG 2 cut(s) 76, 3022
BssT1I CCWWGG 3 cut(s) 2402, 2414, 2820
Bst1107I GTATAC 2 cut(s) 15, 2179
Bst2BI CACGAG 2 cut(s) 76, 3022
Bst2UI CCWGG 3 cut(s) 83, 2396, 3042
Bst4CI ACNGT 3 cut(s) 23, 712, 2742
Bst6I CTCTTC 2 cut(s) 813, 1361
BstACI GRCGYC 1 cut(s) 370
BstAFI CTTAAG 2 cut(s) 1699, 3109
BstAPI GCANNNNNTGC 1 cut(s) 3024
BstBAI YACGTR 1 cut(s) 216
BstBI TTCGAA 2 cut(s) 404, 681
BstC8I GCNNGC 4 cut(s) 383, 449, 832, 2326
BstDEI CTNAG 6 cut(s) 455, 1028, 1130, 1448, 2798, 3089
BstDSI CCRYGG 1 cut(s) 2820
BstEII GGTNACC 2 cut(s) 1013, 1658
BstENI CCTNNNNNAGG 1 cut(s) 2618
BstF5I GGATG 6 cut(s) 1893, 2410, 2425, 2697, 3057, 3067
BstHHI GCGC 1 cut(s) 652
BstKTI GATC 9 cut(s) 75, 112, 154, 246, 1054, 1206, 1257, 2316, 2959
BstMAI GTCTC 6 cut(s) 119, 964, 1403, 2104, 2710, 3087
BstMBI GATC 9 cut(s) 72, 109, 151, 243, 1051, 1203, 1254, 2313, 2956
BstMCI CGRYCG 1 cut(s) 189
BstMWI GCNNNNNNNGC 7 cut(s) 216, 460, 675, 781, 2030, 2863, 3024
BstNI CCWGG 3 cut(s) 83, 2396, 3042
BstNSI RCATGY 3 cut(s) 1916, 2328, 2526
BstPAI GACNNNNGTC 1 cut(s) 1343
BstPI GGTNACC 2 cut(s) 1013, 1658
BstSCI CCNGG 9 cut(s) 49, 50, 81, 1178, 1179, 2394, 2959, 2964, 3040
BstSLI GKGCMC 2 cut(s) 1990, 3088
BstV1I GCAGC 4 cut(s) 463, 759, 1635, 2394
BstX2I RGATCY 2 cut(s) 151, 2956
BstXI CCANNNNNNTGG 3 cut(s) 776, 994, 1307
BstYI RGATCY 2 cut(s) 151, 2956
BstZ17I GTATAC 2 cut(s) 15, 2179
BsuI GTATCC 1 cut(s) 2730
BsuRI GGCC 8 cut(s) 276, 918, 1040, 1746, 2601, 2770, 2945, 2964
BtgI CCRYGG 1 cut(s) 2820
BtsCI GGATG 6 cut(s) 1893, 2410, 2425, 2697, 3057, 3067
BtsI GCAGTG 1 cut(s) 442
BtsIMutI CAGTG 2 cut(s) 442, 2939
BveI ACCTGC 1 cut(s) 1026
Cac8I GCNNGC 4 cut(s) 383, 449, 832, 2326
CciI TCATGA 2 cut(s) 165, 240
CfoI GCGC 1 cut(s) 652
Cfr13I GGNCC 4 cut(s) 275, 2398, 2833, 2963
Cfr9I CCCGGG 2 cut(s) 50, 1179
Csp6I GTAC 9 cut(s) 24, 281, 708, 1047, 1559, 1629, 2189, 2492, 2634
CviQI GTAC 9 cut(s) 24, 281, 708, 1047, 1559, 1629, 2189, 2492, 2634
DdeI CTNAG 6 cut(s) 455, 1028, 1130, 1448, 2798, 3089
DpnI GATC 9 cut(s) 74, 111, 153, 245, 1053, 1205, 1256, 2315, 2958
DpnII GATC 9 cut(s) 72, 109, 151, 243, 1051, 1203, 1254, 2313, 2956
DraI TTTAAA 2 cut(s) 40, 2544
DrdI GACNNNNNNGTC 1 cut(s) 1318
DseDI GACNNNNNNGTC 1 cut(s) 1318
EaeI YGGCCR 3 cut(s) 1038, 1744, 2943
Eam1104I CTCTTC 2 cut(s) 813, 1361
EarI CTCTTC 2 cut(s) 813, 1361
Ecl136II GAGCTC 2 cut(s) 784, 1778
Eco130I CCWWGG 3 cut(s) 2402, 2414, 2820
Eco24I GRGCYC 3 cut(s) 786, 1780, 2859
Eco47I GGWCC 2 cut(s) 2398, 2833
Eco53kI GAGCTC 2 cut(s) 784, 1778
Eco57I CTGAAG 3 cut(s) 1250, 1326, 1563
Eco88I CYCGRG 5 cut(s) 50, 653, 1179, 1400, 2329
Eco91I GGTNACC 2 cut(s) 1013, 1658
EcoICRI GAGCTC 2 cut(s) 784, 1778
EcoNI CCTNNNNNAGG 1 cut(s) 2618
EcoO109I RGGNCCY 1 cut(s) 275
EcoO65I GGTNACC 2 cut(s) 1013, 1658
EcoRI GAATTC 1 cut(s) 406
EcoRII CCWGG 3 cut(s) 81, 2394, 3040
EcoT14I CCWWGG 3 cut(s) 2402, 2414, 2820
EcoT22I ATGCAT 2 cut(s) 71, 2528
EcoT38I GRGCYC 3 cut(s) 786, 1780, 2859
ErhI CCWWGG 3 cut(s) 2402, 2414, 2820
Esp3I CGTCTC 1 cut(s) 119
FalI AAGNNNNNCTT 4 cut(s) 1938, 1970, 2600, 2632
FaqI GGGAC 3 cut(s) 814, 1229, 1324
FauI CCCGC 1 cut(s) 410
FauNDI CATATG 1 cut(s) 646
FbaI TGATCA 1 cut(s) 1203
FblI GTMKAC 5 cut(s) 14, 373, 1909, 2056, 2178
Fnu4HI GCNGC 4 cut(s) 452, 773, 1624, 2408
FokI GGATG 6 cut(s) 1880, 2397, 2432, 2704, 3064, 3074
FriOI GRGCYC 3 cut(s) 786, 1780, 2859
Fsp4HI GCNGC 4 cut(s) 452, 773, 1624, 2408
GlaI GCGC 1 cut(s) 651
GluI GCNGC 4 cut(s) 452, 773, 1624, 2408
GsaI CCCAGC 1 cut(s) 998
GsuI CTGGAG 2 cut(s) 2124, 2664
HaeIII GGCC 8 cut(s) 276, 918, 1040, 1746, 2601, 2770, 2945, 2964
HapII CCGG 9 cut(s) 51, 561, 572, 705, 852, 1180, 2831, 2961, 2966
HhaI GCGC 1 cut(s) 652
Hin1I GRCGYC 1 cut(s) 370
Hin6I GCGC 1 cut(s) 650
HinP1I GCGC 1 cut(s) 650
HincII GTYRAC 3 cut(s) 663, 841, 3073
HindII GTYRAC 3 cut(s) 663, 841, 3073
HindIII AAGCTT 7 cut(s) 667, 676, 1168, 1262, 1414, 1769, 2277
HpaII CCGG 9 cut(s) 51, 561, 572, 705, 852, 1180, 2831, 2961, 2966
Hpy99I CGWCG 2 cut(s) 742, 2912
HpyCH4III ACNGT 3 cut(s) 23, 712, 2742
HpyCH4IV ACGT 5 cut(s) 215, 370, 1906, 2071, 2586
HpyF10VI GCNNNNNNNGC 7 cut(s) 216, 460, 675, 781, 2030, 2863, 3024
HpyF3I CTNAG 6 cut(s) 455, 1028, 1130, 1448, 2798, 3089
HpySE526I ACGT 5 cut(s) 215, 370, 1906, 2071, 2586
Hsp92I GRCGYC 1 cut(s) 370
HspAI GCGC 1 cut(s) 650
Kpn2I TCCGGA 1 cut(s) 571
Ksp22I TGATCA 1 cut(s) 1203
Kzo9I GATC 9 cut(s) 72, 109, 151, 243, 1051, 1203, 1254, 2313, 2956
LguI GCTCTTC 1 cut(s) 1361
LmnI GCTCC 7 cut(s) 4, 789, 1570, 1775, 2246, 2871, 3032
Lsp1109I GCAGC 4 cut(s) 463, 759, 1635, 2394
LweI GCATC 9 cut(s) 698, 973, 1413, 1902, 2214, 2419, 2633, 3027, 3042
MaeII ACGT 5 cut(s) 215, 370, 1906, 2071, 2586
MaeIII GTNAC 7 cut(s) 211, 757, 1013, 1345, 1658, 2591, 2729
MalI GATC 9 cut(s) 74, 111, 153, 245, 1053, 1205, 1256, 2315, 2958
MbiI CCGCTC 1 cut(s) 3049
MboI GATC 9 cut(s) 72, 109, 151, 243, 1051, 1203, 1254, 2313, 2956
MfeI CAATTG 2 cut(s) 1042, 2512
MflI RGATCY 2 cut(s) 151, 2956
MhlI GDGCHC 6 cut(s) 786, 1780, 1990, 2859, 3029, 3088
MlsI TGGCCA 3 cut(s) 1040, 1746, 2945
MluNI TGGCCA 3 cut(s) 1040, 1746, 2945
MlyI GAGTC 4 cut(s) 246, 1112, 1405, 3089
Mox20I TGGCCA 3 cut(s) 1040, 1746, 2945
Mph1103I ATGCAT 2 cut(s) 71, 2528
MroI TCCGGA 1 cut(s) 571
MroXI GAANNNNTTC 2 cut(s) 410, 589
MscI TGGCCA 3 cut(s) 1040, 1746, 2945
MslI CAYNNNNRTG 3 cut(s) 2514, 2589, 2819
Msp20I TGGCCA 3 cut(s) 1040, 1746, 2945
MspA1I CMGCKG 2 cut(s) 772, 1626
MspCI CTTAAG 2 cut(s) 1699, 3109
MspI CCGG 9 cut(s) 51, 561, 572, 705, 852, 1180, 2831, 2961, 2966
MspR9I CCNGG 9 cut(s) 51, 52, 83, 1180, 1181, 2396, 2961, 2966, 3042
MunI CAATTG 2 cut(s) 1042, 2512
Mva1269I GAATGC 4 cut(s) 465, 2276, 2802, 2996
MvaI CCWGG 3 cut(s) 83, 2396, 3042
MwoI GCNNNNNNNGC 7 cut(s) 216, 460, 675, 781, 2030, 2863, 3024
NciI CCSGG 6 cut(s) 51, 52, 1180, 1181, 2961, 2966
NcoI CCATGG 1 cut(s) 2820
NdeI CATATG 1 cut(s) 646
NdeII GATC 9 cut(s) 72, 109, 151, 243, 1051, 1203, 1254, 2313, 2956
NlaIV GGNNCC 8 cut(s) 6, 277, 714, 1456, 2297, 2400, 2662, 2958
NmeAIII GCCGAG 1 cut(s) 1599
NmuCI GTSAC 3 cut(s) 1013, 1345, 2591
NsiI ATGCAT 2 cut(s) 71, 2528
NspI RCATGY 3 cut(s) 1916, 2328, 2526
NspV TTCGAA 2 cut(s) 404, 681
OliI CACNNNNGTG 2 cut(s) 2514, 2589
PaeI GCATGC 1 cut(s) 2328
PaeR7I CTCGAG 1 cut(s) 2329
PagI TCATGA 2 cut(s) 165, 240
PciI ACATGT 1 cut(s) 1912
PciSI GCTCTTC 1 cut(s) 1361
PctI GAATGC 4 cut(s) 465, 2276, 2802, 2996
PdmI GAANNNNTTC 2 cut(s) 410, 589
PfeI GAWTC 9 cut(s) 86, 182, 318, 1189, 1291, 1750, 2084, 2120, 2429
PflFI GACNNNGTC 1 cut(s) 2837
PflMI CCANNNNNTGG 2 cut(s) 538, 2687
PkrI GCNGC 4 cut(s) 453, 774, 1625, 2409
PleI GAGTC 4 cut(s) 245, 1111, 1404, 3089
PpsI GAGTC 4 cut(s) 245, 1111, 1404, 3089
Ppu21I YACGTR 1 cut(s) 216
PscI ACATGT 1 cut(s) 1912
PshAI GACNNNNGTC 1 cut(s) 1343
PshBI ATTAAT 1 cut(s) 1997
Psp124BI GAGCTC 2 cut(s) 786, 1780
Psp6I CCWGG 3 cut(s) 81, 2394, 3040
PspEI GGTNACC 2 cut(s) 1013, 1658
PspFI CCCAGC 1 cut(s) 994
PspGI CCWGG 3 cut(s) 81, 2394, 3040
PspN4I GGNNCC 8 cut(s) 6, 277, 714, 1456, 2297, 2400, 2662, 2958
PspPI GGNCC 4 cut(s) 275, 2398, 2833, 2963
PspXI VCTCGAGB 1 cut(s) 2329
PsuI RGATCY 2 cut(s) 151, 2956
PsyI GACNNNGTC 1 cut(s) 2837
PvuII CAGCTG 2 cut(s) 772, 1626
RsaI GTAC 9 cut(s) 25, 282, 709, 1048, 1560, 1630, 2190, 2493, 2635
RsaNI GTAC 9 cut(s) 24, 281, 708, 1047, 1559, 1629, 2189, 2492, 2634
RseI CAYNNNNRTG 3 cut(s) 2514, 2589, 2819
SacI GAGCTC 2 cut(s) 786, 1780
SapI GCTCTTC 1 cut(s) 1361
SatI GCNGC 4 cut(s) 452, 773, 1624, 2408
Sau3AI GATC 9 cut(s) 72, 109, 151, 243, 1051, 1203, 1254, 2313, 2956
Sau96I GGNCC 4 cut(s) 275, 2398, 2833, 2963
ScaI AGTACT 1 cut(s) 2190
SchI GAGTC 4 cut(s) 246, 1112, 1405, 3089
ScrFI CCNGG 9 cut(s) 51, 52, 83, 1180, 1181, 2396, 2961, 2966, 3042
SduI GDGCHC 6 cut(s) 786, 1780, 1990, 2859, 3029, 3088
SfaNI GCATC 9 cut(s) 698, 973, 1413, 1902, 2214, 2419, 2633, 3027, 3042
Sfr274I CTCGAG 1 cut(s) 2329
SfuI TTCGAA 2 cut(s) 404, 681
SinI GGWCC 2 cut(s) 2398, 2833
SlaI CTCGAG 1 cut(s) 2329
SmaI CCCGGG 2 cut(s) 52, 1181
SmiMI CAYNNNNRTG 3 cut(s) 2514, 2589, 2819
SmlI CTYRAG 8 cut(s) 357, 818, 1055, 1699, 1944, 2309, 2329, 3109
SmoI CTYRAG 8 cut(s) 357, 818, 1055, 1699, 1944, 2309, 2329, 3109
SpeI ACTAGT 1 cut(s) 1631
SphI GCATGC 1 cut(s) 2328
SsiI CCGC 9 cut(s) 284, 417, 658, 834, 1887, 2299, 2817, 3030, 3049
SspI AATATT 3 cut(s) 1795, 2470, 2557
SstI GAGCTC 2 cut(s) 786, 1780
StyD4I CCNGG 9 cut(s) 49, 50, 81, 1178, 1179, 2394, 2959, 2964, 3040
StyI CCWWGG 3 cut(s) 2402, 2414, 2820
TaaI ACNGT 3 cut(s) 23, 712, 2742
TaiI ACGT 5 cut(s) 218, 373, 1909, 2074, 2589
TaqI TCGA 9 cut(s) 189, 404, 681, 740, 892, 1121, 1511, 2330, 2871
TaqII GACCGA 1 cut(s) 175
TatI WGTACW 4 cut(s) 1046, 1628, 2188, 2491
TfiI GAWTC 9 cut(s) 86, 182, 318, 1189, 1291, 1750, 2084, 2120, 2429
TscAI CASTG 2 cut(s) 449, 2946
TseFI GTSAC 3 cut(s) 1013, 1345, 2591
TseI GCWGC 4 cut(s) 451, 772, 1623, 2407
Tsp45I GTSAC 3 cut(s) 1013, 1345, 2591
TspGWI ACGGA 1 cut(s) 2925
TspMI CCCGGG 2 cut(s) 50, 1179
TspRI CASTG 2 cut(s) 449, 2946
Tth111I GACNNNGTC 1 cut(s) 2837
Van91I CCANNNNNTGG 2 cut(s) 538, 2687
Vha464I CTTAAG 2 cut(s) 1699, 3109
VneI GTGCAC 2 cut(s) 1986, 3084
VpaK11BI GGWCC 2 cut(s) 2398, 2833
VspI ATTAAT 1 cut(s) 1997
XagI CCTNNNNNAGG 1 cut(s) 2618
XceI RCATGY 3 cut(s) 1916, 2328, 2526
XcmI CCANNNNNNNNNTGG 1 cut(s) 539
XhoI CTCGAG 1 cut(s) 2329
XmaI CCCGGG 2 cut(s) 50, 1179
XmiI GTMKAC 5 cut(s) 14, 373, 1909, 2056, 2178
XmnI GAANNNNTTC 2 cut(s) 410, 589
ZraI GACGTC 1 cut(s) 371
ZrmI AGTACT 1 cut(s) 2190
Zsp2I ATGCAT 2 cut(s) 71, 2528
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.