MD12G1134700.v1.1

LRR receptor-like serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr12
OG0000262
Physical Location & Seq
Reverse (-)
21082391 .. 21083600
1210 bp
Loading structure...
UTR
Exon/CDS
Intron
MD12G1134700.v1.1.491

Sequence Viewer

Length: 936 bp
ATGTTATGCTTTATTGTTGCTCGTTCAATGCTTAAGAAGCCAAGAGGTATAACTGAAAGCTCACGTTCTTATAGGGATTGGAAATTAAGTGTCTCTTACTCAGAACTTGTTGAATCAACTAACAAGTTCTCTAGAGAAAATCTAATTGGTTCAGGAAGTTTTGGTTCTGTTTACAAAGGAGTACTCCCTAGCGATGGAAGTGTAGTTGCAGTTAAGGTATTAAATCTTCAACAACAAGGAGCTTTTAAGAGTTTCATTCGTGAATGTGAAGCTTTAAGAAGTTACATGGTAAATGGAAGTTTAGACGTGCGGTTGCATGATGAGGAATCTCAAAGCAAGAGGTTGAGCCTTATCCAAAGACTCAATATTGCAATTGATGTTGCTTCTGCATTGGATTACCTCCACCACCATTGCGATACGACAATTGTTCACTGTGATCTAAAGTCAAGCAATGTTCTCCTTGATCAAGATATGGTGGCTCATGTTGGTTACTTTGGTTTAGCAAGGTTCCTATTGGAAGCGGCGGACAATCCCTCTCAAAGTCAAACCATGTTAGCGGGGCTAAAAGGTTCCATTGCCTACATTCCTCCGGATGTTTATAGCTTTGGAATACTGCTGCTAGAAATGTTCACGGGAAAAAGACCTACCGATGGAATGCTTGGAGATGGTATAAGTATTCACAATTTCACAGCCATGGCAATGCCTAATCATGCCACGGACATAGTTGACGCTTCATTGCTCATTGAAGGAGAGGATGCAGATGATATTGATGACAGGTACGAAAAGCAGGTACAAGAAAAACCTATTAAAATGCGTCATAACCACGGCCTACTCCAAGGAAGAAAATTGGAGGAATGTTTAGCTTCAGTGATTTGCAATATCTCCAAGAGAGCAGATGCATATGGATATTCTTGTCAACAAAATGAAGGCAGTTAG

Protein Analysis

312

Amino Acids

34.48

Weight (kDa)

5.81

Isoelectric Point (pI)

43.58

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pkinase PF00069 44 - 96 2e-06 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 94 - 221 2.3e-17 Protein tyrosine and serine/threonine kinase
Pkinase PF00069 95 - 231 1.2e-14 Protein kinase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000262)

Species Orthologous Gene IDs
fragaria_vesca FvH4_6g11680 FvH4_6g11680 FvH4_6g11680 FvH4_6g11680 FvH4_6g11680 FvH4_6g11680 FvH4_6g11681 FvH4_6g11682 FvH4_6g11683
malus_domestica MD00G1079100.v1.1 MD00G1080000.v1.1 MD01G1100900.v1.1 MD03G1225200.v1.1 MD04G1041600.v1.1 MD04G1110500.v1.1 MD04G1118700.v1.1 MD04G1150300.v1.1 MD04G1150400.v1.1 MD04G1156000.v1.1 MD04G1156200.v1.1 MD04G1156700.v1.1 MD04G1157400.v1.1 MD04G1157500.v1.1 MD04G1157700.v1.1 MD04G1158000.v1.1 MD04G1158200.v1.1 MD04G1158600.v1.1 MD04G1159000.v1.1 MD04G1159100.v1.1 MD05G1061600.v1.1 MD05G1075800.v1.1 MD06G1129300.v1.1 MD07G1217800.v1.1 MD07G1218000.v1.1 MD07G1308700.v1.1 MD12G1130700.v1.1 MD12G1130800.v1.1 MD12G1134500.v1.1 MD12G1134700.v1.1 MD12G1135000.v1.1 MD12G1135200.v1.1 MD12G1135800.v1.1 MD12G1164300.v1.1 MD12G1164400.v1.1 MD12G1169100.v1.1 MD12G1169700.v1.1 MD12G1170200.v1.1 MD12G1170400.v1.1 MD13G1275300.v1.1
prunus_persica Prupe.1G072900_v2.0.a1 Prupe.1G194200_v2.0.a1 Prupe.1G201100_v2.0.a1 Prupe.6G248000_v2.0.a1 Prupe.6G248100_v2.0.a1 Prupe.6G248500_v2.0.a1 Prupe.6G274800_v2.0.a1 Prupe.6G274900_v2.0.a1 Prupe.6G275100_v2.0.a1 Prupe.6G275100_v2.0.a1 Prupe.7G044200_v2.0.a1 Prupe.7G044300_v2.0.a1 Prupe.7G044700_v2.0.a1
pyrus_communis pycom04g03530 pycom04g10490 pycom04g10500 pycom04g10510 pycom04g10560 pycom04g13580 pycom04g13600 pycom04g14080 pycom12g10580 pycom12g11020 pycom12g12650 pycom12g12680 pycom12g15730 pycom12g15740 pycom12g16060 pycom12g16100 pycom12g16200 pycom12g16240 pycom16g21060
rosa_chinensis RchiOBHm_Chr3g0463401 RchiOBHm_Chr3g0463411 RchiOBHm_Chr3g0463481 RchiOBHm_Chr4g0408571 RchiOBHm_Chr4g0408641
rosa_laevigata RLG00000019546 RLG00000019547 RLG00000019552 RLG00000019553 RLG00000019554 RLG00000019578 RLG00000024784 RLG00000024785 RLG00000024786
rosa_multiflora Rmu_sc0000923.1_g000013 Rmu_sc0000923.1_g000017 Rmu_sc0000923.1_g000018 Rmu_sc0001053.1_g000005 Rmu_sc0001053.1_g000012 Rmu_sc0001053.1_g000013 Rmu_sc0001053.1_g000014 Rmu_sc0031971.1_g000001
rosa_roxburghii Rroxscaffold_2G00107980 Rroxscaffold_6G00416540 Rroxscaffold_6G00416560
rosa_rugosa Rorug02G0333800 Rorug02G0333900 Rorug03G0064500 Rorug03G0064600 Rorug03G0064800 Rorug03G0064900 Rorug03G0065000 Rorug03G0065000
rosa_samantha Rh2CG370900 Rh3AG122100 Rh3BG126500 Rh3BG126800 Rh3BG126900 Rh3CG128600 Rh3CG128700 Rh3DG127400 Rh3DG127500 Rh3DG127700 Rh4CG158500 Rh4CG158600
rosa_wichuraiana Rw0G010610 Rw0G010620 Rw3G010370 Rw3G010380 Rw3G010400 Rw3G010410

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 778
AccIII TCCGGA 1 cut(s) 589
AciI CCGC 4 cut(s) 310, 521, 524, 557
AcuI CTGAAG 1 cut(s) 849
AfaI GTAC 3 cut(s) 183, 779, 792
AfiI CCNNNNNNNGG 2 cut(s) 194, 650
AflII CTTAAG 1 cut(s) 32
AgsI TTSAA 4 cut(s) 27, 113, 230, 746
AjiI CACGTC 1 cut(s) 307
AjuI GAANNNNNNNTTGG 2 cut(s) 129, 161
AluBI AGCT 5 cut(s) 60, 242, 272, 603, 863
AluI AGCT 5 cut(s) 60, 242, 272, 603, 863
Alw26I GTCTC 1 cut(s) 97
Aor13HI TCCGGA 1 cut(s) 589
AoxI GGCC 1 cut(s) 826
ApeKI GCWGC 1 cut(s) 616
BarI GAAGNNNNNNTAC 2 cut(s) 210, 242
BbvI GCAGC 1 cut(s) 603
BccI CCATC 3 cut(s) 188, 644, 659
BceAI ACGGC 1 cut(s) 841
BclI TGATCA 1 cut(s) 463
BcoDI GTCTC 1 cut(s) 97
BfaI CTAG 3 cut(s) 132, 189, 620
BfrI CTTAAG 1 cut(s) 32
BfuAI ACCTGC 1 cut(s) 778
BisI GCNGC 2 cut(s) 522, 617
BlsI GCNGC 2 cut(s) 523, 618
BmcAI AGTACT 1 cut(s) 183
BmgBI CACGTC 1 cut(s) 307
BmiI GGNNCC 2 cut(s) 509, 571
BmsI GCATC 2 cut(s) 745, 886
BsaJI CCNNGG 4 cut(s) 693, 714, 823, 835
BsaWI WCCGGW 1 cut(s) 589
Bsc4I CCNNNNNNNGG 2 cut(s) 194, 650
Bse3DI GCAATG 5 cut(s) 409, 457, 573, 705, 734
BseAI TCCGGA 1 cut(s) 589
BseDI CCNNGG 4 cut(s) 693, 714, 823, 835
BseGI GGATG 2 cut(s) 598, 760
BseLI CCNNNNNNNGG 2 cut(s) 194, 650
BseMI GCAATG 5 cut(s) 409, 457, 573, 705, 734
BseMII CTCAG 1 cut(s) 114
BseXI GCAGC 1 cut(s) 603
BshFI GGCC 1 cut(s) 828
BsiSI CCGG 1 cut(s) 590
BslI CCNNNNNNNGG 2 cut(s) 194, 650
BsmAI GTCTC 1 cut(s) 97
BsmI GAATGC 1 cut(s) 660
BsnI GGCC 1 cut(s) 828
Bsp13I TCCGGA 1 cut(s) 589
Bsp143I GATC 2 cut(s) 436, 463
Bsp19I CCATGG 1 cut(s) 693
BspACI CCGC 4 cut(s) 310, 521, 524, 557
BspANI GGCC 1 cut(s) 828
BspCNI CTCAG 1 cut(s) 113
BspEI TCCGGA 1 cut(s) 589
BspLI GGNNCC 2 cut(s) 509, 571
BspMI ACCTGC 1 cut(s) 778
BspTI CTTAAG 1 cut(s) 32
BsrDI GCAATG 5 cut(s) 409, 457, 573, 705, 734
BssECI CCNNGG 4 cut(s) 693, 714, 823, 835
BssMI GATC 2 cut(s) 436, 463
BssT1I CCWWGG 2 cut(s) 693, 835
Bst4CI ACNGT 1 cut(s) 434
BstAFI CTTAAG 1 cut(s) 32
BstDEI CTNAG 1 cut(s) 100
BstDSI CCRYGG 3 cut(s) 693, 714, 823
BstF5I GGATG 2 cut(s) 598, 760
BstKTI GATC 2 cut(s) 439, 466
BstMAI GTCTC 1 cut(s) 97
BstMBI GATC 2 cut(s) 436, 463
BstMWI GCNNNNNNNGC 1 cut(s) 37
BstV1I GCAGC 1 cut(s) 603
BsuRI GGCC 1 cut(s) 828
BtgI CCRYGG 3 cut(s) 693, 714, 823
BtgZI GCGATG 1 cut(s) 207
BtrI CACGTC 1 cut(s) 307
BtsCI GGATG 2 cut(s) 598, 760
BtsIMutI CAGTG 2 cut(s) 430, 873
BveI ACCTGC 1 cut(s) 778
CseI GACGC 2 cut(s) 737, 803
Csp6I GTAC 3 cut(s) 182, 778, 791
CspCI CAANNNNNGTGG 2 cut(s) 392, 427
CviAII CATG 6 cut(s) 286, 317, 482, 550, 694, 710
CviQI GTAC 3 cut(s) 182, 778, 791
DdeI CTNAG 1 cut(s) 100
DpnI GATC 2 cut(s) 438, 465
DpnII GATC 2 cut(s) 436, 463
EciI GGCGGA 1 cut(s) 539
Eco130I CCWWGG 2 cut(s) 693, 835
Eco57I CTGAAG 1 cut(s) 849
EcoT14I CCWWGG 2 cut(s) 693, 835
EcoT22I ATGCAT 1 cut(s) 901
ErhI CCWWGG 2 cut(s) 693, 835
FaeI CATG 6 cut(s) 289, 320, 485, 553, 697, 713
FalI AAGNNNNNCTT 2 cut(s) 79, 111
FatI CATG 6 cut(s) 285, 316, 481, 549, 693, 709
FauI CCCGC 1 cut(s) 550
FauNDI CATATG 1 cut(s) 901
FbaI TGATCA 1 cut(s) 463
Fnu4HI GCNGC 2 cut(s) 522, 617
FokI GGATG 2 cut(s) 605, 767
Fsp4HI GCNGC 2 cut(s) 522, 617
FspBI CTAG 3 cut(s) 132, 189, 620
GluI GCNGC 2 cut(s) 522, 617
HaeIII GGCC 1 cut(s) 828
HapII CCGG 1 cut(s) 590
HgaI GACGC 2 cut(s) 737, 803
Hin1II CATG 6 cut(s) 289, 320, 485, 553, 697, 713
HincII GTYRAC 2 cut(s) 727, 917
HindII GTYRAC 2 cut(s) 727, 917
HindIII AAGCTT 1 cut(s) 270
HinfI GANTC 3 cut(s) 113, 326, 360
HpaII CCGG 1 cut(s) 590
Hpy166II GTNNAC 5 cut(s) 172, 430, 630, 727, 917
Hpy188I TCNGA 1 cut(s) 103
Hpy188III TCNNGA 5 cut(s) 132, 153, 260, 467, 590
Hpy8I GTNNAC 5 cut(s) 172, 430, 630, 727, 917
HpyAV CCTTC 2 cut(s) 740, 920
HpyCH4III ACNGT 1 cut(s) 434
HpyCH4IV ACGT 2 cut(s) 64, 306
HpyCH4V TGCA 7 cut(s) 209, 316, 371, 389, 758, 876, 899
HpyF10VI GCNNNNNNNGC 1 cut(s) 37
HpyF3I CTNAG 1 cut(s) 100
HpySE526I ACGT 2 cut(s) 64, 306
Hsp92II CATG 6 cut(s) 289, 320, 485, 553, 697, 713
Kpn2I TCCGGA 1 cut(s) 589
Ksp22I TGATCA 1 cut(s) 463
Kzo9I GATC 2 cut(s) 436, 463
LmnI GCTCC 1 cut(s) 239
LpnPI CCDG 4 cut(s) 138, 603, 760, 773
Lsp1109I GCAGC 1 cut(s) 603
LweI GCATC 2 cut(s) 745, 886
MaeI CTAG 3 cut(s) 132, 189, 620
MaeII ACGT 2 cut(s) 64, 306
MaeIII GTNAC 2 cut(s) 281, 488
MalI GATC 2 cut(s) 438, 465
MboI GATC 2 cut(s) 436, 463
MboII GAAGA 2 cut(s) 218, 852
MfeI CAATTG 2 cut(s) 372, 423
MluCI AATT 6 cut(s) 83, 144, 372, 423, 682, 845
MlyI GAGTC 1 cut(s) 354
MnlI CCTC 8 cut(s) 38, 316, 333, 410, 544, 597, 745, 844
Mph1103I ATGCAT 1 cut(s) 901
MroI TCCGGA 1 cut(s) 589
MseI TTAA 7 cut(s) 33, 86, 213, 221, 246, 275, 807
MslI CAYNNNNRTG 2 cut(s) 692, 698
MspCI CTTAAG 1 cut(s) 32
MspI CCGG 1 cut(s) 590
MunI CAATTG 2 cut(s) 372, 423
Mva1269I GAATGC 1 cut(s) 660
MwoI GCNNNNNNNGC 1 cut(s) 37
NcoI CCATGG 1 cut(s) 693
NdeI CATATG 1 cut(s) 901
NdeII GATC 2 cut(s) 436, 463
NlaIII CATG 6 cut(s) 289, 320, 485, 553, 697, 713
NlaIV GGNNCC 2 cut(s) 509, 571
NsiI ATGCAT 1 cut(s) 901
PctI GAATGC 1 cut(s) 660
PfeI GAWTC 2 cut(s) 113, 326
PkrI GCNGC 2 cut(s) 523, 618
PleI GAGTC 1 cut(s) 354
PpsI GAGTC 1 cut(s) 354
PspN4I GGNNCC 2 cut(s) 509, 571
RsaI GTAC 3 cut(s) 183, 779, 792
RsaNI GTAC 3 cut(s) 182, 778, 791
RseI CAYNNNNRTG 2 cut(s) 692, 698
SaqAI TTAA 7 cut(s) 33, 86, 213, 221, 246, 275, 807
SatI GCNGC 2 cut(s) 522, 617
Sau3AI GATC 2 cut(s) 436, 463
ScaI AGTACT 1 cut(s) 183
SchI GAGTC 1 cut(s) 354
SfaNI GCATC 2 cut(s) 745, 886
SmiMI CAYNNNNRTG 2 cut(s) 692, 698
SmlI CTYRAG 1 cut(s) 32
SmoI CTYRAG 1 cut(s) 32
Sse9I AATT 6 cut(s) 83, 144, 372, 423, 682, 845
SsiI CCGC 4 cut(s) 310, 521, 524, 557
SspI AATATT 1 cut(s) 367
SspMI CTAG 3 cut(s) 132, 189, 620
StyI CCWWGG 2 cut(s) 693, 835
TaaI ACNGT 1 cut(s) 434
TaiI ACGT 2 cut(s) 67, 309
TasI AATT 6 cut(s) 83, 144, 372, 423, 682, 845
TatI WGTACW 1 cut(s) 181
TauI GCSGC 1 cut(s) 524
TfiI GAWTC 2 cut(s) 113, 326
Tru1I TTAA 7 cut(s) 33, 86, 213, 221, 246, 275, 807
Tru9I TTAA 7 cut(s) 33, 86, 213, 221, 246, 275, 807
TscAI CASTG 2 cut(s) 437, 873
TseI GCWGC 1 cut(s) 616
TspDTI ATGAA 2 cut(s) 244, 723
TspGWI ACGGA 1 cut(s) 731
TspRI CASTG 2 cut(s) 437, 873
Vha464I CTTAAG 1 cut(s) 32
XbaI TCTAGA 1 cut(s) 131
XspI CTAG 3 cut(s) 132, 189, 620
ZrmI AGTACT 1 cut(s) 183
Zsp2I ATGCAT 1 cut(s) 901
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.