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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AluBI | AGCT | 1 cut(s) | 207 |
| AluI | AGCT | 1 cut(s) | 207 |
| ApeKI | GCWGC | 1 cut(s) | 207 |
| BbvI | GCAGC | 1 cut(s) | 194 |
| BccI | CCATC | 1 cut(s) | 9 |
| BfaI | CTAG | 1 cut(s) | 42 |
| BisI | GCNGC | 1 cut(s) | 208 |
| BlsI | GCNGC | 1 cut(s) | 209 |
| BseGI | GGATG | 1 cut(s) | 20 |
| BseXI | GCAGC | 1 cut(s) | 194 |
| BstF5I | GGATG | 1 cut(s) | 20 |
| BstV1I | GCAGC | 1 cut(s) | 194 |
| BtsCI | GGATG | 1 cut(s) | 20 |
| CviJI | RGCY | 2 cut(s) | 41, 207 |
| CviKI_1 | RGCY | 2 cut(s) | 41, 207 |
| DraI | TTTAAA | 2 cut(s) | 27, 34 |
| FaiI | YATR | 2 cut(s) | 9, 61 |
| Fnu4HI | GCNGC | 1 cut(s) | 208 |
| FokI | GGATG | 1 cut(s) | 27 |
| Fsp4HI | GCNGC | 1 cut(s) | 208 |
| FspBI | CTAG | 1 cut(s) | 42 |
| GluI | GCNGC | 1 cut(s) | 208 |
| HinfI | GANTC | 1 cut(s) | 94 |
| Hpy188III | TCNNGA | 1 cut(s) | 76 |
| HpyCH4V | TGCA | 1 cut(s) | 210 |
| LmnI | GCTCC | 1 cut(s) | 4 |
| LpnPI | CCDG | 3 cut(s) | 83, 209, 217 |
| Lsp1109I | GCAGC | 1 cut(s) | 194 |
| MaeI | CTAG | 1 cut(s) | 42 |
| MboII | GAAGA | 1 cut(s) | 76 |
| MmeI | TCCRAC | 1 cut(s) | 165 |
| MnlI | CCTC | 3 cut(s) | 31, 78, 101 |
| MseI | TTAA | 3 cut(s) | 26, 33, 122 |
| MspA1I | CMGCKG | 1 cut(s) | 207 |
| PfeI | GAWTC | 1 cut(s) | 94 |
| PkrI | GCNGC | 1 cut(s) | 209 |
| PvuII | CAGCTG | 1 cut(s) | 207 |
| SaqAI | TTAA | 3 cut(s) | 26, 33, 122 |
| SatI | GCNGC | 1 cut(s) | 208 |
| SetI | ASST | 4 cut(s) | 102, 132, 198, 209 |
| SgeI | CNNG | 6 cut(s) | 54, 88, 93, 110, 208, 216 |
| SspMI | CTAG | 1 cut(s) | 42 |
| TfiI | GAWTC | 1 cut(s) | 94 |
| Tru1I | TTAA | 3 cut(s) | 26, 33, 122 |
| Tru9I | TTAA | 3 cut(s) | 26, 33, 122 |
| TseI | GCWGC | 1 cut(s) | 207 |
| TspDTI | ATGAA | 1 cut(s) | 84 |
| XspI | CTAG | 1 cut(s) | 42 |