pycom13g28180

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr13
OG0000608
Physical Location & Seq
Forward (+)
24128867 .. 24130088
1222 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom13g28180.2

Sequence Viewer

Length: 789 bp
ATGGGTGAAAGACTAGTTAGAGGGTCCTTTTCCACACATGACATATGCCCCAAATTAACCATGAAAAGGGATACACATCACAACCAAATTATCCCTATCAAGTTCTCTAACTATGAAAAGCATGATAGAAAATCATATCAAAGGTCATTAAGTTCTTTGAAAACCATAAGCATTGACAAGGCATTCGTAACTATGAAAATTCATAACGATTGGGTGAAATTCCCTTATACTCTAGCATCAAATTCATGCATGCAAATTAAGTGTCGACTCCCAATCCACATACATAAACAAGTTTTAATAACTCAGATAACAATTTATATAAAACATATAATCATGGCTTCGAATTCACCTCTAACTAAAAAGAAATTAGTTCCACATAAAGAACCAAGAAACGCTCCACACTCCAATGGCATGCATGACACTCCCTTGGATGGCAGATTGCACAAGTCTTCTTCTCCTTCCTTCCTTGCTTGCGGCACTAGGGTGATGTGGTGTGAATTATGGTTAAAACCTGCGGCAAAGGCTTGTATTTATAGGCTAGGTTTTGCACAATTTCTGAAGGAAAATGATTGCACAATCCTAAGGGAAAAAGGTTATGGTTTGGCAGAAACCAAGGGGGAAAGGGATAGGGAATACACGGTAGGGATTTGGTGTAGTATATGTATAAGTAGGGATCGTTCTGGACCGGGGATTAGGAGGGATTACTCAAATATTGCAAAACTAAACTTTAAAACATTAAAACAAACCAAAACACTCAAACAGTACCCAAAGCACTCGAAACTGCCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

263

Amino Acids

30.25

Weight (kDa)

9.9

Isoelectric Point (pI)

40.35

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 520
AccI GTMKAC 1 cut(s) 265
AciI CCGC 2 cut(s) 474, 515
AclWI GGATC 1 cut(s) 681
AcsI RAATTY 4 cut(s) 198, 218, 241, 343
AcuI CTGAAG 1 cut(s) 578
AfaI GTAC 1 cut(s) 764
AfiI CCNNNNNNNGG 2 cut(s) 66, 431
AgsI TTSAA 1 cut(s) 160
AhlI ACTAGT 1 cut(s) 13
AleI CACNNNNGTG 1 cut(s) 482
AlwI GGATC 1 cut(s) 681
ApoI RAATTY 4 cut(s) 198, 218, 241, 343
ArsI GACNNNNNNTTYG 4 cut(s) 167, 199, 247, 279
AspS9I GGNCC 2 cut(s) 24, 683
AsuC2I CCSGG 1 cut(s) 687
AsuHPI GGTGA 4 cut(s) 17, 226, 339, 496
AsuII TTCGAA 1 cut(s) 341
AvaII GGWCC 2 cut(s) 24, 683
AxyI CCTNAGG 1 cut(s) 581
BbsI GAAGAC 1 cut(s) 441
BccI CCATC 1 cut(s) 425
BciVI GTATCC 1 cut(s) 64
BcnI CCSGG 1 cut(s) 687
BcuI ACTAGT 1 cut(s) 13
BfaI CTAG 4 cut(s) 14, 233, 480, 539
BfuAI ACCTGC 1 cut(s) 520
BfuI GTATCC 1 cut(s) 64
BisI GCNGC 2 cut(s) 475, 516
BlsI GCNGC 2 cut(s) 476, 517
Bme1390I CCNGG 1 cut(s) 687
Bme18I GGWCC 2 cut(s) 24, 683
BmgT120I GGNCC 2 cut(s) 24, 683
BmiI GGNNCC 1 cut(s) 25
BmrFI CCNGG 1 cut(s) 687
BmsI GCATC 1 cut(s) 245
BpiI GAAGAC 1 cut(s) 441
Bpu14I TTCGAA 1 cut(s) 341
BpuMI CCSGG 1 cut(s) 687
BsaBI GATNNNNATC 1 cut(s) 75
BsaJI CCNNGG 3 cut(s) 426, 612, 686
Bsc4I CCNNNNNNNGG 2 cut(s) 66, 431
Bse21I CCTNAGG 1 cut(s) 581
Bse8I GATNNNNATC 1 cut(s) 75
BseDI CCNNGG 3 cut(s) 426, 612, 686
BseGI GGATG 1 cut(s) 436
BseJI GATNNNNATC 1 cut(s) 75
BseLI CCNNNNNNNGG 2 cut(s) 66, 431
BseMII CTCAG 1 cut(s) 317
BsiSI CCGG 1 cut(s) 686
BslI CCNNNNNNNGG 2 cut(s) 66, 431
BsmI GAATGC 1 cut(s) 182
Bsp119I TTCGAA 1 cut(s) 341
Bsp143I GATC 1 cut(s) 673
BspACI CCGC 2 cut(s) 474, 515
BspCNI CTCAG 1 cut(s) 316
BspLI GGNNCC 1 cut(s) 25
BspMI ACCTGC 1 cut(s) 520
BspPI GGATC 1 cut(s) 681
BspT104I TTCGAA 1 cut(s) 341
BssECI CCNNGG 3 cut(s) 426, 612, 686
BssMI GATC 1 cut(s) 673
BssT1I CCWWGG 2 cut(s) 426, 612
Bst4CI ACNGT 2 cut(s) 640, 762
BstBI TTCGAA 1 cut(s) 341
BstC8I GCNNGC 3 cut(s) 251, 413, 472
BstDEI CTNAG 2 cut(s) 303, 581
BstF5I GGATG 1 cut(s) 436
BstKTI GATC 1 cut(s) 676
BstMBI GATC 1 cut(s) 673
BstMWI GCNNNNNNNGC 1 cut(s) 521
BstNSI RCATGY 2 cut(s) 253, 415
BstSCI CCNGG 1 cut(s) 685
BstV2I GAAGAC 1 cut(s) 441
Bsu36I CCTNAGG 1 cut(s) 581
BsuI GTATCC 1 cut(s) 64
BtsCI GGATG 1 cut(s) 436
BveI ACCTGC 1 cut(s) 520
Cac8I GCNNGC 3 cut(s) 251, 413, 472
Cfr13I GGNCC 2 cut(s) 24, 683
Csp6I GTAC 1 cut(s) 763
CviAII CATG 8 cut(s) 38, 61, 122, 246, 250, 334, 412, 416
CviJI RGCY 3 cut(s) 338, 524, 538
CviKI_1 RGCY 3 cut(s) 338, 524, 538
CviQI GTAC 1 cut(s) 763
DdeI CTNAG 2 cut(s) 303, 581
DpnI GATC 1 cut(s) 675
DpnII GATC 1 cut(s) 673
DraI TTTAAA 1 cut(s) 730
Eco130I CCWWGG 2 cut(s) 426, 612
Eco47I GGWCC 2 cut(s) 24, 683
Eco57I CTGAAG 1 cut(s) 578
Eco81I CCTNAGG 1 cut(s) 581
EcoO109I RGGNCCY 1 cut(s) 24
EcoRI GAATTC 1 cut(s) 343
EcoT14I CCWWGG 2 cut(s) 426, 612
EcoT22I ATGCAT 2 cut(s) 251, 417
ErhI CCWWGG 2 cut(s) 426, 612
FaeI CATG 8 cut(s) 41, 64, 125, 249, 253, 337, 415, 419
FatI CATG 8 cut(s) 37, 60, 121, 245, 249, 333, 411, 415
FauNDI CATATG 1 cut(s) 44
FblI GTMKAC 1 cut(s) 265
Fnu4HI GCNGC 2 cut(s) 475, 516
FokI GGATG 1 cut(s) 443
Fsp4HI GCNGC 2 cut(s) 475, 516
FspBI CTAG 4 cut(s) 14, 233, 480, 539
GluI GCNGC 2 cut(s) 475, 516
HapII CCGG 1 cut(s) 686
Hin1II CATG 8 cut(s) 41, 64, 125, 249, 253, 337, 415, 419
HincII GTYRAC 1 cut(s) 266
HindII GTYRAC 1 cut(s) 266
HinfI GANTC 1 cut(s) 267
HpaII CCGG 1 cut(s) 686
HphI GGTGA 4 cut(s) 17, 226, 339, 496
Hpy166II GTNNAC 1 cut(s) 266
Hpy188I TCNGA 2 cut(s) 306, 558
Hpy188III TCNNGA 1 cut(s) 681
Hpy8I GTNNAC 1 cut(s) 266
HpyAV CCTTC 3 cut(s) 468, 472, 553
HpyCH4III ACNGT 2 cut(s) 640, 762
HpyCH4V TGCA 7 cut(s) 249, 253, 415, 442, 548, 573, 716
HpyF10VI GCNNNNNNNGC 1 cut(s) 521
HpyF3I CTNAG 2 cut(s) 303, 581
Hsp92II CATG 8 cut(s) 41, 64, 125, 249, 253, 337, 415, 419
Kzo9I GATC 1 cut(s) 673
LmnI GCTCC 1 cut(s) 400
LpnPI CCDG 3 cut(s) 525, 666, 699
LweI GCATC 1 cut(s) 245
MaeI CTAG 4 cut(s) 14, 233, 480, 539
MaeIII GTNAC 1 cut(s) 187
MalI GATC 1 cut(s) 675
MboI GATC 1 cut(s) 673
MboII GAAGA 2 cut(s) 441, 444
MlyI GAGTC 1 cut(s) 261
MnlI CCTC 3 cut(s) 14, 360, 690
Mph1103I ATGCAT 2 cut(s) 251, 417
MseI TTAA 8 cut(s) 56, 149, 258, 296, 506, 729, 737, 787
MslI CAYNNNNRTG 2 cut(s) 405, 482
MspI CCGG 1 cut(s) 686
MspR9I CCNGG 1 cut(s) 687
Mva1269I GAATGC 1 cut(s) 182
MwoI GCNNNNNNNGC 1 cut(s) 521
NciI CCSGG 1 cut(s) 687
NdeI CATATG 1 cut(s) 44
NdeII GATC 1 cut(s) 673
NlaIII CATG 8 cut(s) 41, 64, 125, 249, 253, 337, 415, 419
NlaIV GGNNCC 1 cut(s) 25
NsiI ATGCAT 2 cut(s) 251, 417
NspI RCATGY 2 cut(s) 253, 415
NspV TTCGAA 1 cut(s) 341
OliI CACNNNNGTG 1 cut(s) 482
PaeI GCATGC 2 cut(s) 253, 415
PctI GAATGC 1 cut(s) 182
PkrI GCNGC 2 cut(s) 476, 517
PleI GAGTC 1 cut(s) 261
PpsI GAGTC 1 cut(s) 261
PpuMI RGGWCCY 1 cut(s) 24
Psp5II RGGWCCY 1 cut(s) 24
PspN4I GGNNCC 1 cut(s) 25
PspPI GGNCC 2 cut(s) 24, 683
PspPPI RGGWCCY 1 cut(s) 24
PsrI GAACNNNNNNTAC 2 cut(s) 661, 693
RsaI GTAC 1 cut(s) 764
RsaNI GTAC 1 cut(s) 763
RseI CAYNNNNRTG 2 cut(s) 405, 482
SalI GTCGAC 1 cut(s) 264
SaqAI TTAA 8 cut(s) 56, 149, 258, 296, 506, 729, 737, 787
SatI GCNGC 2 cut(s) 475, 516
Sau3AI GATC 1 cut(s) 673
Sau96I GGNCC 2 cut(s) 24, 683
SchI GAGTC 1 cut(s) 261
ScrFI CCNGG 1 cut(s) 687
SetI ASST 5 cut(s) 146, 352, 514, 544, 595
SfaNI GCATC 1 cut(s) 245
SfuI TTCGAA 1 cut(s) 341
SinI GGWCC 2 cut(s) 24, 683
SmiMI CAYNNNNRTG 2 cut(s) 405, 482
SpeI ACTAGT 1 cut(s) 13
SphI GCATGC 2 cut(s) 253, 415
SsiI CCGC 2 cut(s) 474, 515
SspI AATATT 1 cut(s) 712
SspMI CTAG 4 cut(s) 14, 233, 480, 539
StyD4I CCNGG 1 cut(s) 685
StyI CCWWGG 2 cut(s) 426, 612
TaaI ACNGT 2 cut(s) 640, 762
TaqI TCGA 3 cut(s) 265, 341, 776
TauI GCSGC 2 cut(s) 477, 518
Tru1I TTAA 8 cut(s) 56, 149, 258, 296, 506, 729, 737, 787
Tru9I TTAA 8 cut(s) 56, 149, 258, 296, 506, 729, 737, 787
TspDTI ATGAA 5 cut(s) 77, 129, 191, 209, 234
VpaK11BI GGWCC 2 cut(s) 24, 683
XapI RAATTY 4 cut(s) 198, 218, 241, 343
XceI RCATGY 2 cut(s) 253, 415
XmiI GTMKAC 1 cut(s) 265
XspI CTAG 4 cut(s) 14, 233, 480, 539
Zsp2I ATGCAT 2 cut(s) 251, 417
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.