pycom05g08270

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr5
OG0000608
Physical Location & Seq
Forward (+)
10935526 .. 10936755
1230 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom05g08270.6

Sequence Viewer

Length: 726 bp
ATGCATAGGGGTACGGCATTGTCCAATCCCGAGGCTGAAAGTTCCTTGATTGTTCGATTATCCGCAGCCATGAGTTGTTCTTCCTCTTGCTCTTCTTTTTTGTCAAACTCAGATTCAAAGCTAGAACTGGGTGGATTAGGTTTTGGCTCCTTCCTCTTTCATCTCAAAGTTTGCTCAAAATCGTCGTCAAACTCCAAGATATGTCAACAAAACAAAACAAAACAAAAATTAGAAACTAAAACTAAGAGATACGAACAGAAACAAAGGGGATTAGCAAAATTGCTAATCCCCGTAGGGATCGTTATAGCCCGGGGATTAGAAGGTATGCTAATCTCTCTAGAAACTAAAGCAGTACAAAACAAGAAAATTGACTCAAAACAGAAACTAAGAATAAAGAGGGGAATTGGTTTTGACGAATTTAAAACTCAAACAAACAAATTGCAGAAACTAGAGGGTTCTTCTCCACACATGACACATATGACAATGTCCCAAGTTAATCGTGAACTCAAATTATTCTTATCAAGTTCTCTATATGAACAACATGATAGAGATACATATCAAAGACCATTAAGTTCTATGGAAATCATAAGCATTGACATGGCATTCGTAACTATGAAAAGCATGATACTTTTGCCAGGAATTTACTTAACCCGAACCCCTTATATTTTAGCATCAAATTCCTACATGCAAACTAAGTATGCATCCTTAATCAATATACAAGAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

242

Amino Acids

27.05

Weight (kDa)

9.74

Isoelectric Point (pI)

43.67

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 63
AclWI GGATC 1 cut(s) 305
AcsI RAATTY 3 cut(s) 416, 639, 676
AfaI GTAC 2 cut(s) 13, 354
AgsI TTSAA 1 cut(s) 117
AjnI CCWGG 1 cut(s) 634
AluBI AGCT 1 cut(s) 121
AluI AGCT 1 cut(s) 121
AlwI GGATC 1 cut(s) 305
Ama87I CYCGRG 2 cut(s) 29, 309
ApeKI GCWGC 1 cut(s) 65
ApoI RAATTY 3 cut(s) 416, 639, 676
ArsI GACNNNNNNTTYG 4 cut(s) 170, 202, 587, 619
AsuC2I CCSGG 2 cut(s) 310, 311
AvaI CYCGRG 2 cut(s) 29, 309
BbvI GCAGC 1 cut(s) 77
BceAI ACGGC 1 cut(s) 30
BciT130I CCWGG 1 cut(s) 636
BcnI CCSGG 2 cut(s) 310, 311
BfaI CTAG 3 cut(s) 122, 338, 449
BisI GCNGC 1 cut(s) 66
BlsI GCNGC 1 cut(s) 67
Bme1390I CCNGG 3 cut(s) 310, 311, 636
BmeT110I CYCGRG 2 cut(s) 29, 309
BmiI GGNNCC 1 cut(s) 148
BmrFI CCNGG 3 cut(s) 310, 311, 636
BmrI ACTGGG 1 cut(s) 137
BmsI GCATC 2 cut(s) 680, 710
BmuI ACTGGG 1 cut(s) 137
BpuMI CCSGG 2 cut(s) 310, 311
BsaBI GATNNNNATC 1 cut(s) 555
BsaJI CCNNGG 3 cut(s) 30, 309, 310
Bse1I ACTGG 1 cut(s) 132
Bse8I GATNNNNATC 1 cut(s) 555
BseBI CCWGG 1 cut(s) 636
BseDI CCNNGG 3 cut(s) 30, 309, 310
BseGI GGATG 1 cut(s) 701
BseJI GATNNNNATC 1 cut(s) 555
BseMII CTCAG 1 cut(s) 123
BseNI ACTGG 1 cut(s) 132
BseXI GCAGC 1 cut(s) 77
BsiHKCI CYCGRG 2 cut(s) 29, 309
BsiSI CCGG 1 cut(s) 310
BslFI GGGAC 1 cut(s) 472
BsmFI GGGAC 1 cut(s) 472
BsmI GAATGC 1 cut(s) 602
BsoBI CYCGRG 2 cut(s) 29, 309
Bsp143I GATC 1 cut(s) 297
BspACI CCGC 1 cut(s) 63
BspCNI CTCAG 1 cut(s) 122
BspLI GGNNCC 1 cut(s) 148
BspPI GGATC 1 cut(s) 305
BspQI GCTCTTC 1 cut(s) 97
BsrI ACTGG 1 cut(s) 132
BssECI CCNNGG 3 cut(s) 30, 309, 310
BssMI GATC 1 cut(s) 297
Bst2UI CCWGG 1 cut(s) 636
Bst6I CTCTTC 1 cut(s) 97
BstDEI CTNAG 4 cut(s) 109, 243, 386, 693
BstF5I GGATG 1 cut(s) 701
BstKTI GATC 1 cut(s) 300
BstMBI GATC 1 cut(s) 297
BstNI CCWGG 1 cut(s) 636
BstNSI RCATGY 1 cut(s) 688
BstSCI CCNGG 3 cut(s) 308, 309, 634
BstV1I GCAGC 1 cut(s) 77
BtsCI GGATG 1 cut(s) 701
Cfr9I CCCGGG 1 cut(s) 309
Csp6I GTAC 2 cut(s) 12, 353
CviAII CATG 6 cut(s) 70, 469, 542, 598, 622, 685
CviJI RGCY 5 cut(s) 35, 68, 121, 147, 308
CviKI_1 RGCY 5 cut(s) 35, 68, 121, 147, 308
CviQI GTAC 2 cut(s) 12, 353
DdeI CTNAG 4 cut(s) 109, 243, 386, 693
DpnI GATC 1 cut(s) 299
DpnII GATC 1 cut(s) 297
DraI TTTAAA 1 cut(s) 421
Eam1104I CTCTTC 1 cut(s) 97
EarI CTCTTC 1 cut(s) 97
Eco88I CYCGRG 2 cut(s) 29, 309
EcoRII CCWGG 1 cut(s) 634
EcoT22I ATGCAT 2 cut(s) 6, 703
FaeI CATG 6 cut(s) 73, 472, 545, 601, 625, 688
FaqI GGGAC 1 cut(s) 472
FatI CATG 6 cut(s) 69, 468, 541, 597, 621, 684
FauNDI CATATG 1 cut(s) 477
Fnu4HI GCNGC 1 cut(s) 66
FokI GGATG 1 cut(s) 688
Fsp4HI GCNGC 1 cut(s) 66
FspBI CTAG 3 cut(s) 122, 338, 449
GluI GCNGC 1 cut(s) 66
HapII CCGG 1 cut(s) 310
Hin1II CATG 6 cut(s) 73, 472, 545, 601, 625, 688
HincII GTYRAC 1 cut(s) 206
HindII GTYRAC 1 cut(s) 206
HinfI GANTC 2 cut(s) 113, 371
HpaII CCGG 1 cut(s) 310
Hpy166II GTNNAC 2 cut(s) 206, 503
Hpy188I TCNGA 1 cut(s) 112
Hpy188III TCNNGA 3 cut(s) 29, 338, 500
Hpy8I GTNNAC 2 cut(s) 206, 503
Hpy99I CGWCG 1 cut(s) 187
HpyAV CCTTC 2 cut(s) 160, 314
HpyCH4V TGCA 4 cut(s) 4, 442, 688, 701
HpyF3I CTNAG 4 cut(s) 109, 243, 386, 693
Hsp92II CATG 6 cut(s) 73, 472, 545, 601, 625, 688
Kzo9I GATC 1 cut(s) 297
LguI GCTCTTC 1 cut(s) 97
LmnI GCTCC 1 cut(s) 152
LpnPI CCDG 4 cut(s) 113, 323, 621, 648
Lsp1109I GCAGC 1 cut(s) 77
LweI GCATC 2 cut(s) 680, 710
MaeI CTAG 3 cut(s) 122, 338, 449
MaeIII GTNAC 1 cut(s) 607
MalI GATC 1 cut(s) 299
MboI GATC 1 cut(s) 297
MboII GAAGA 3 cut(s) 72, 84, 450
MluCI AATT 9 cut(s) 227, 278, 366, 402, 416, 437, 509, 639, 676
MlyI GAGTC 1 cut(s) 365
MnlI CCTC 5 cut(s) 25, 94, 164, 390, 445
Mph1103I ATGCAT 2 cut(s) 6, 703
MseI TTAA 5 cut(s) 420, 495, 569, 647, 707
MslI CAYNNNNRTG 1 cut(s) 596
MspI CCGG 1 cut(s) 310
MspR9I CCNGG 3 cut(s) 310, 311, 636
Mva1269I GAATGC 1 cut(s) 602
MvaI CCWGG 1 cut(s) 636
NciI CCSGG 2 cut(s) 310, 311
NdeI CATATG 1 cut(s) 477
NdeII GATC 1 cut(s) 297
NlaIII CATG 6 cut(s) 73, 472, 545, 601, 625, 688
NlaIV GGNNCC 1 cut(s) 148
NsiI ATGCAT 2 cut(s) 6, 703
NspI RCATGY 1 cut(s) 688
PciSI GCTCTTC 1 cut(s) 97
PctI GAATGC 1 cut(s) 602
PfeI GAWTC 1 cut(s) 113
PflFI GACNNNGTC 1 cut(s) 484
PkrI GCNGC 1 cut(s) 67
PleI GAGTC 1 cut(s) 365
PpsI GAGTC 1 cut(s) 365
Psp6I CCWGG 1 cut(s) 634
PspGI CCWGG 1 cut(s) 634
PspN4I GGNNCC 1 cut(s) 148
PsyI GACNNNGTC 1 cut(s) 484
RsaI GTAC 2 cut(s) 13, 354
RsaNI GTAC 2 cut(s) 12, 353
RseI CAYNNNNRTG 1 cut(s) 596
SapI GCTCTTC 1 cut(s) 97
SaqAI TTAA 5 cut(s) 420, 495, 569, 647, 707
SatI GCNGC 1 cut(s) 66
Sau3AI GATC 1 cut(s) 297
SchI GAGTC 1 cut(s) 365
ScrFI CCNGG 3 cut(s) 310, 311, 636
SetI ASST 3 cut(s) 123, 142, 325
SfaNI GCATC 2 cut(s) 680, 710
SmaI CCCGGG 1 cut(s) 311
SmiMI CAYNNNNRTG 1 cut(s) 596
Sse9I AATT 9 cut(s) 227, 278, 366, 402, 416, 437, 509, 639, 676
SsiI CCGC 1 cut(s) 63
SspMI CTAG 3 cut(s) 122, 338, 449
StyD4I CCNGG 3 cut(s) 308, 309, 634
TaqI TCGA 1 cut(s) 55
TasI AATT 9 cut(s) 227, 278, 366, 402, 416, 437, 509, 639, 676
TatI WGTACW 1 cut(s) 352
TfiI GAWTC 1 cut(s) 113
Tru1I TTAA 5 cut(s) 420, 495, 569, 647, 707
Tru9I TTAA 5 cut(s) 420, 495, 569, 647, 707
TseI GCWGC 1 cut(s) 65
TspDTI ATGAA 3 cut(s) 149, 549, 629
TspMI CCCGGG 1 cut(s) 309
Tth111I GACNNNGTC 1 cut(s) 484
XapI RAATTY 3 cut(s) 416, 639, 676
XbaI TCTAGA 1 cut(s) 337
XceI RCATGY 1 cut(s) 688
XmaI CCCGGG 1 cut(s) 309
XspI CTAG 3 cut(s) 122, 338, 449
Zsp2I ATGCAT 2 cut(s) 6, 703
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.