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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AanI | TTATAA | 1 cut(s) | 285 |
| AclWI | GGATC | 1 cut(s) | 214 |
| AcoI | YGGCCR | 2 cut(s) | 55, 151 |
| AcsI | RAATTY | 2 cut(s) | 225, 441 |
| AgsI | TTSAA | 5 cut(s) | 118, 374, 513, 534, 616 |
| AlwI | GGATC | 1 cut(s) | 214 |
| AoxI | GGCC | 3 cut(s) | 55, 151, 503 |
| ApoI | RAATTY | 2 cut(s) | 225, 441 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 441 |
| AspLEI | GCGC | 1 cut(s) | 23 |
| AspS9I | GGNCC | 2 cut(s) | 503, 522 |
| AsuHPI | GGTGA | 1 cut(s) | 133 |
| AvaII | GGWCC | 1 cut(s) | 522 |
| BccI | CCATC | 1 cut(s) | 545 |
| BceAI | ACGGC | 1 cut(s) | 70 |
| BclI | TGATCA | 1 cut(s) | 471 |
| BfaI | CTAG | 2 cut(s) | 266, 417 |
| Bme18I | GGWCC | 1 cut(s) | 522 |
| BmgT120I | GGNCC | 2 cut(s) | 503, 522 |
| BmiI | GGNNCC | 2 cut(s) | 101, 524 |
| BmsI | GCATC | 2 cut(s) | 170, 278 |
| BpuEI | CTTGAG | 1 cut(s) | 462 |
| Bsa29I | ATCGAT | 2 cut(s) | 333, 383 |
| BsaJI | CCNNGG | 1 cut(s) | 58 |
| Bse1I | ACTGG | 1 cut(s) | 249 |
| BseCI | ATCGAT | 2 cut(s) | 333, 383 |
| BseDI | CCNNGG | 1 cut(s) | 58 |
| BseNI | ACTGG | 1 cut(s) | 249 |
| BshFI | GGCC | 3 cut(s) | 57, 153, 505 |
| BshVI | ATCGAT | 2 cut(s) | 333, 383 |
| BsmI | GAATGC | 2 cut(s) | 54, 86 |
| BsnI | GGCC | 3 cut(s) | 57, 153, 505 |
| Bsp143I | GATC | 5 cut(s) | 219, 330, 334, 384, 471 |
| BspANI | GGCC | 3 cut(s) | 57, 153, 505 |
| BspDI | ATCGAT | 2 cut(s) | 333, 383 |
| BspHI | TCATGA | 1 cut(s) | 337 |
| BspLI | GGNNCC | 2 cut(s) | 101, 524 |
| BspPI | GGATC | 1 cut(s) | 214 |
| BsrI | ACTGG | 1 cut(s) | 249 |
| BssECI | CCNNGG | 1 cut(s) | 58 |
| BssMI | GATC | 5 cut(s) | 219, 330, 334, 384, 471 |
| BssT1I | CCWWGG | 1 cut(s) | 58 |
| BstDEI | CTNAG | 1 cut(s) | 499 |
| BstHHI | GCGC | 1 cut(s) | 23 |
| BstKTI | GATC | 5 cut(s) | 222, 333, 337, 387, 474 |
| BstMBI | GATC | 5 cut(s) | 219, 330, 334, 384, 471 |
| BstNSI | RCATGY | 3 cut(s) | 74, 78, 88 |
| Bsu15I | ATCGAT | 2 cut(s) | 333, 383 |
| BsuRI | GGCC | 3 cut(s) | 57, 153, 505 |
| BsuTUI | ATCGAT | 2 cut(s) | 333, 383 |
| CciI | TCATGA | 1 cut(s) | 337 |
| CfoI | GCGC | 1 cut(s) | 23 |
| Cfr13I | GGNCC | 2 cut(s) | 503, 522 |
| ClaI | ATCGAT | 2 cut(s) | 333, 383 |
| CviAII | CATG | 5 cut(s) | 71, 75, 85, 257, 338 |
| CviJI | RGCY | 6 cut(s) | 57, 153, 238, 416, 428, 505 |
| CviKI_1 | RGCY | 6 cut(s) | 57, 153, 238, 416, 428, 505 |
| DdeI | CTNAG | 1 cut(s) | 499 |
| DpnI | GATC | 5 cut(s) | 221, 332, 336, 386, 473 |
| DpnII | GATC | 5 cut(s) | 219, 330, 334, 384, 471 |
| DraI | TTTAAA | 1 cut(s) | 136 |
| EaeI | YGGCCR | 2 cut(s) | 55, 151 |
| Eco130I | CCWWGG | 1 cut(s) | 58 |
| Eco47I | GGWCC | 1 cut(s) | 522 |
| EcoRI | GAATTC | 1 cut(s) | 441 |
| EcoT14I | CCWWGG | 1 cut(s) | 58 |
| EcoT22I | ATGCAT | 2 cut(s) | 76, 86 |
| ErhI | CCWWGG | 1 cut(s) | 58 |
| FaeI | CATG | 5 cut(s) | 74, 78, 88, 260, 341 |
| FatI | CATG | 5 cut(s) | 70, 74, 84, 256, 337 |
| FauNDI | CATATG | 1 cut(s) | 278 |
| FbaI | TGATCA | 1 cut(s) | 471 |
| FspBI | CTAG | 2 cut(s) | 266, 417 |
| GlaI | GCGC | 1 cut(s) | 22 |
| HaeIII | GGCC | 3 cut(s) | 57, 153, 505 |
| HhaI | GCGC | 1 cut(s) | 23 |
| Hin1II | CATG | 5 cut(s) | 74, 78, 88, 260, 341 |
| Hin6I | GCGC | 1 cut(s) | 21 |
| HinP1I | GCGC | 1 cut(s) | 21 |
| HincII | GTYRAC | 1 cut(s) | 465 |
| HindII | GTYRAC | 1 cut(s) | 465 |
| HinfI | GANTC | 2 cut(s) | 486, 616 |
| HphI | GGTGA | 1 cut(s) | 133 |
| Hpy166II | GTNNAC | 4 cut(s) | 36, 68, 254, 465 |
| Hpy188III | TCNNGA | 4 cut(s) | 187, 304, 338, 388 |
| Hpy8I | GTNNAC | 4 cut(s) | 36, 68, 254, 465 |
| HpyCH4V | TGCA | 4 cut(s) | 52, 74, 84, 161 |
| HpyF3I | CTNAG | 1 cut(s) | 499 |
| Hsp92II | CATG | 5 cut(s) | 74, 78, 88, 260, 341 |
| HspAI | GCGC | 1 cut(s) | 21 |
| Ksp22I | TGATCA | 1 cut(s) | 471 |
| Kzo9I | GATC | 5 cut(s) | 219, 330, 334, 384, 471 |
| LpnPI | CCDG | 3 cut(s) | 172, 262, 289 |
| LweI | GCATC | 2 cut(s) | 170, 278 |
| MaeI | CTAG | 2 cut(s) | 266, 417 |
| MaeIII | GTNAC | 1 cut(s) | 407 |
| MalI | GATC | 5 cut(s) | 221, 332, 336, 386, 473 |
| MboI | GATC | 5 cut(s) | 219, 330, 334, 384, 471 |
| MboII | GAAGA | 3 cut(s) | 522, 590, 593 |
| MluCI | AATT | 5 cut(s) | 108, 156, 225, 352, 441 |
| MmeI | TCCRAC | 1 cut(s) | 77 |
| MnlI | CCTC | 1 cut(s) | 24 |
| Mph1103I | ATGCAT | 2 cut(s) | 76, 86 |
| MroXI | GAANNNNTTC | 1 cut(s) | 441 |
| MseI | TTAA | 2 cut(s) | 135, 168 |
| MslI | CAYNNNNRTG | 2 cut(s) | 75, 79 |
| Mva1269I | GAATGC | 2 cut(s) | 54, 86 |
| NdeI | CATATG | 1 cut(s) | 278 |
| NdeII | GATC | 5 cut(s) | 219, 330, 334, 384, 471 |
| NlaIII | CATG | 5 cut(s) | 74, 78, 88, 260, 341 |
| NlaIV | GGNNCC | 2 cut(s) | 101, 524 |
| NsiI | ATGCAT | 2 cut(s) | 76, 86 |
| NspI | RCATGY | 3 cut(s) | 74, 78, 88 |
| PagI | TCATGA | 1 cut(s) | 337 |
| PctI | GAATGC | 2 cut(s) | 54, 86 |
| PdmI | GAANNNNTTC | 1 cut(s) | 441 |
| PfeI | GAWTC | 2 cut(s) | 486, 616 |
| PsiI | TTATAA | 1 cut(s) | 285 |
| PspN4I | GGNNCC | 2 cut(s) | 101, 524 |
| PspPI | GGNCC | 2 cut(s) | 503, 522 |
| RseI | CAYNNNNRTG | 2 cut(s) | 75, 79 |
| SaqAI | TTAA | 2 cut(s) | 135, 168 |
| Sau3AI | GATC | 5 cut(s) | 219, 330, 334, 384, 471 |
| Sau96I | GGNCC | 2 cut(s) | 503, 522 |
| SetI | ASST | 3 cut(s) | 35, 64, 406 |
| SfaNI | GCATC | 2 cut(s) | 170, 278 |
| SinI | GGWCC | 1 cut(s) | 522 |
| SmiMI | CAYNNNNRTG | 2 cut(s) | 75, 79 |
| SmlI | CTYRAG | 1 cut(s) | 477 |
| SmoI | CTYRAG | 1 cut(s) | 477 |
| Sse9I | AATT | 5 cut(s) | 108, 156, 225, 352, 441 |
| SspMI | CTAG | 2 cut(s) | 266, 417 |
| StyI | CCWWGG | 1 cut(s) | 58 |
| TaqI | TCGA | 2 cut(s) | 333, 383 |
| TasI | AATT | 5 cut(s) | 108, 156, 225, 352, 441 |
| TfiI | GAWTC | 2 cut(s) | 486, 616 |
| Tru1I | TTAA | 2 cut(s) | 135, 168 |
| Tru9I | TTAA | 2 cut(s) | 135, 168 |
| TspDTI | ATGAA | 6 cut(s) | 192, 273, 338, 354, 450, 544 |
| VpaK11BI | GGWCC | 1 cut(s) | 522 |
| XapI | RAATTY | 2 cut(s) | 225, 441 |
| XceI | RCATGY | 3 cut(s) | 74, 78, 88 |
| XmnI | GAANNNNTTC | 1 cut(s) | 441 |
| XspI | CTAG | 2 cut(s) | 266, 417 |
| Zsp2I | ATGCAT | 2 cut(s) | 76, 86 |