pycom17g18240

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr17
OG0000608
Physical Location & Seq
Forward (+)
15661230 .. 15663128
1899 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom17g18240.13

Sequence Viewer

Length: 1254 bp
ATGTGTTTTAAGGCTGAAATTAGGAATGTGATCTTGGGACACAAAATAGGGAATAAGCACTCCCCTTGCACGGCAAGGAAGGGACAAGTTAAGGCAAGCCACGGCAAGGGTTGGAATGGTGCAAGGAAGGAAAGGTCAAACCAAAATAGGAAACCTTGGCTTAACTTTCCTACTTCAAGTAGGAATCCATCTTTGACTAGGAATCCTTCTTTGATTAGGAATCCTTCTTTGATTAGGAATCCTAACATCTTTAGGTCTTCAATTTCGTCCATTCCTTTTGCTCCATGCATATGCTATCCATTCCAAGTCCAATTTTGCTCCAAAAGGCTCCAAAATGCACCTTCTTGCTACTTTAGCCATATGGACCTACAAACACACGAAAATAAAAACAAGCTAACTAAGTCGCCTAAATATGCTCCTATCAATTACTACAGTTCATTCCATGTACTACGTCTCCCTCTCTCCTCTCTTAGGCCTGGTTTGGTACTGAGCTTCGGCTTTTTTTCACAGTTTTGGGTGAAAAGAAGCCAAAAACAAGAAGTTGCAAAACCCAGCTTTGAAAAACCGGCTTTTTTTCACATCTCTGCTTATTCTCATAGCACAGTAGAAGTAGCTTTTTTTTCAAAGCACAGCAATACCAAACCAGCCCTTAGTTATTGTTCTCTATTTGTAAGTGATGAGGTCTTTAGGTTTTCACATTCGTTGATCGTGAATTTGATTTCAATTTGTTATATCAGATTGTTGTATAAAAAAAATGTGTGCAAAAAATATTTTGTTTTTTGTTTTAAACTTTTTAATGTGTTACAAATCAACGAATTAACTAATATAAATTTAAAATTACAATTTAATCGAATTAGAGTTCATGTTGAATTCATAAAAAAGTCGACTCGTATTTGCATACAGATTAAACTAGAAATCGAAGCTCGAAACACAGGGATTGAACTTGGTTTGGAACTTCAAACCCCCAAACCTTGTTCGAATTTAATAACGATTGATGTTGTTTTATATAATTGTACAAAGTCATTGAATGCTGCTGGGCCTTTCTTGTCTCTAAAGCAACAAACTACAGCTCAGCCAGGGCCAATATATGTAGTTCTTGCAGTGCTGAATCCTTCTTCTTTGCAGTCTCTGCATGGAACCTGGGAATTTGATACTTTGTTTTGCTTGGTTTGGTTTGTTCTCATGCAAAAATTACAAATATTTATTTTGTTTTATTTAGTGATCTTGCTCTTCGTTGCTGATGCATGCATATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

418

Amino Acids

47.84

Weight (kDa)

9.85

Isoelectric Point (pI)

46.29

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 884
AcsI RAATTY 5 cut(s) 712, 829, 869, 979, 1145
AfaI GTAC 3 cut(s) 447, 486, 1015
AfiI CCNNNNNNNGG 3 cut(s) 70, 106, 471
AgsI TTSAA 9 cut(s) 177, 261, 560, 624, 723, 869, 941, 959, 1027
AjnI CCWGG 3 cut(s) 475, 1075, 1139
AjuI GAANNNNNNNTTGG 2 cut(s) 17, 49
AluBI AGCT 6 cut(s) 394, 492, 555, 614, 923, 1070
AluI AGCT 6 cut(s) 394, 492, 555, 614, 923, 1070
Alw26I GTCTC 3 cut(s) 458, 1053, 1131
AlwNI CAGNNNCTG 1 cut(s) 1129
AoxI GGCC 3 cut(s) 473, 1037, 1079
ApeKI GCWGC 1 cut(s) 1031
ApoI RAATTY 5 cut(s) 712, 829, 869, 979, 1145
AspS9I GGNCC 3 cut(s) 364, 1037, 1079
AsuHPI GGTGA 1 cut(s) 529
AsuII TTCGAA 1 cut(s) 977
AvaII GGWCC 1 cut(s) 364
BbsI GAAGAC 1 cut(s) 249
BbvI GCAGC 1 cut(s) 1018
BccI CCATC 1 cut(s) 196
BceAI ACGGC 2 cut(s) 87, 118
BciT130I CCWGG 3 cut(s) 477, 1077, 1141
BcoDI GTCTC 3 cut(s) 458, 1053, 1131
BfaI CTAG 2 cut(s) 198, 911
BfmI CTRYAG 2 cut(s) 430, 1065
BisI GCNGC 1 cut(s) 1032
BlpI GCTNAGC 1 cut(s) 1071
BlsI GCNGC 1 cut(s) 1033
Bme1390I CCNGG 3 cut(s) 477, 1077, 1141
Bme18I GGWCC 1 cut(s) 364
BmgT120I GGNCC 3 cut(s) 364, 1037, 1079
BmiI GGNNCC 2 cut(s) 329, 1138
BmrFI CCNGG 3 cut(s) 477, 1077, 1141
BmsI GCATC 1 cut(s) 1231
BpiI GAAGAC 1 cut(s) 249
Bpu1102I GCTNAGC 1 cut(s) 1071
Bpu14I TTCGAA 1 cut(s) 977
BsaJI CCNNGG 4 cut(s) 100, 155, 1076, 1140
BsaXI ACNNNNNCTCC 2 cut(s) 438, 468
Bsc4I CCNNNNNNNGG 3 cut(s) 70, 106, 471
Bse118I RCCGGY 1 cut(s) 565
BseBI CCWGG 3 cut(s) 477, 1077, 1141
BseDI CCNNGG 4 cut(s) 100, 155, 1076, 1140
BseLI CCNNNNNNNGG 3 cut(s) 70, 106, 471
BseMII CTCAG 2 cut(s) 479, 1085
BseRI GAGGAG 1 cut(s) 454
BseXI GCAGC 1 cut(s) 1018
BseYI CCCAGC 2 cut(s) 551, 1034
BshFI GGCC 3 cut(s) 475, 1039, 1081
BsiSI CCGG 1 cut(s) 566
BslFI GGGAC 2 cut(s) 51, 96
BslI CCNNNNNNNGG 3 cut(s) 70, 106, 471
BsmAI GTCTC 3 cut(s) 458, 1053, 1131
BsmBI CGTCTC 1 cut(s) 458
BsmFI GGGAC 2 cut(s) 51, 96
BsmI GAATGC 1 cut(s) 1033
BsnI GGCC 3 cut(s) 475, 1039, 1081
Bsp119I TTCGAA 1 cut(s) 977
Bsp1407I TGTACA 1 cut(s) 1013
Bsp143I GATC 3 cut(s) 30, 705, 1221
Bsp1720I GCTNAGC 1 cut(s) 1071
BspANI GGCC 3 cut(s) 475, 1039, 1081
BspCNI CTCAG 2 cut(s) 480, 1084
BspLI GGNNCC 2 cut(s) 329, 1138
BspQI GCTCTTC 1 cut(s) 1235
BspT104I TTCGAA 1 cut(s) 977
BsrFI RCCGGY 1 cut(s) 565
BsrGI TGTACA 1 cut(s) 1013
BssAI RCCGGY 1 cut(s) 565
BssECI CCNNGG 4 cut(s) 100, 155, 1076, 1140
BssMI GATC 3 cut(s) 30, 705, 1221
BssT1I CCWWGG 1 cut(s) 155
Bst2UI CCWGG 3 cut(s) 477, 1077, 1141
Bst4CI ACNGT 3 cut(s) 434, 510, 604
Bst6I CTCTTC 1 cut(s) 1235
BstAPI GCANNNNNTGC 1 cut(s) 1129
BstAUI TGTACA 1 cut(s) 1013
BstBI TTCGAA 1 cut(s) 977
BstC8I GCNNGC 2 cut(s) 97, 1246
BstDEI CTNAG 5 cut(s) 399, 470, 488, 650, 1071
BstDSI CCRYGG 1 cut(s) 100
BstENI CCTNNNNNAGG 1 cut(s) 469
BstKTI GATC 3 cut(s) 33, 708, 1224
BstMAI GTCTC 3 cut(s) 458, 1053, 1131
BstMBI GATC 3 cut(s) 30, 705, 1221
BstMWI GCNNNNNNNGC 2 cut(s) 354, 1129
BstNI CCWGG 3 cut(s) 477, 1077, 1141
BstNSI RCATGY 1 cut(s) 1248
BstSCI CCNGG 3 cut(s) 475, 1075, 1139
BstSFI CTRYAG 2 cut(s) 430, 1065
BstV1I GCAGC 1 cut(s) 1018
BstV2I GAAGAC 1 cut(s) 249
BsuRI GGCC 3 cut(s) 475, 1039, 1081
BtgI CCRYGG 1 cut(s) 100
BtsI GCAGTG 1 cut(s) 1107
BtsIMutI CAGTG 1 cut(s) 1107
Cac8I GCNNGC 2 cut(s) 97, 1246
CaiI CAGNNNCTG 1 cut(s) 1129
Cfr10I RCCGGY 1 cut(s) 565
Cfr13I GGNCC 3 cut(s) 364, 1037, 1079
Csp6I GTAC 3 cut(s) 446, 485, 1014
CviAII CATG 6 cut(s) 285, 443, 863, 1133, 1183, 1245
CviQI GTAC 3 cut(s) 446, 485, 1014
DdeI CTNAG 5 cut(s) 399, 470, 488, 650, 1071
DpnI GATC 3 cut(s) 32, 707, 1223
DpnII GATC 3 cut(s) 30, 705, 1221
DraI TTTAAA 2 cut(s) 787, 834
Eam1104I CTCTTC 1 cut(s) 1235
EarI CTCTTC 1 cut(s) 1235
Eco130I CCWWGG 1 cut(s) 155
Eco147I AGGCCT 1 cut(s) 475
Eco47I GGWCC 1 cut(s) 364
EcoNI CCTNNNNNAGG 1 cut(s) 469
EcoRI GAATTC 1 cut(s) 869
EcoRII CCWGG 3 cut(s) 475, 1075, 1139
EcoT14I CCWWGG 1 cut(s) 155
EcoT22I ATGCAT 3 cut(s) 290, 1246, 1250
ErhI CCWWGG 1 cut(s) 155
Esp3I CGTCTC 1 cut(s) 458
FaeI CATG 6 cut(s) 288, 446, 866, 1136, 1186, 1248
FaqI GGGAC 2 cut(s) 51, 96
FatI CATG 6 cut(s) 284, 442, 862, 1132, 1182, 1244
FauNDI CATATG 3 cut(s) 290, 360, 1250
FblI GTMKAC 1 cut(s) 884
Fnu4HI GCNGC 1 cut(s) 1032
Fsp4HI GCNGC 1 cut(s) 1032
FspBI CTAG 2 cut(s) 198, 911
GluI GCNGC 1 cut(s) 1032
GsaI CCCAGC 2 cut(s) 555, 1038
HaeIII GGCC 3 cut(s) 475, 1039, 1081
HapII CCGG 1 cut(s) 566
Hin1II CATG 6 cut(s) 288, 446, 866, 1136, 1186, 1248
HincII GTYRAC 1 cut(s) 885
HindII GTYRAC 1 cut(s) 885
HinfI GANTC 6 cut(s) 184, 202, 220, 238, 886, 1108
HpaII CCGG 1 cut(s) 566
HphI GGTGA 1 cut(s) 529
Hpy166II GTNNAC 1 cut(s) 885
Hpy188I TCNGA 1 cut(s) 737
Hpy188III TCNNGA 1 cut(s) 709
Hpy8I GTNNAC 1 cut(s) 885
HpyAV CCTTC 6 cut(s) 73, 121, 216, 234, 351, 1122
HpyCH4III ACNGT 3 cut(s) 434, 510, 604
HpyCH4IV ACGT 1 cut(s) 451
HpyF10VI GCNNNNNNNGC 2 cut(s) 354, 1129
HpyF3I CTNAG 5 cut(s) 399, 470, 488, 650, 1071
HpySE526I ACGT 1 cut(s) 451
Hsp92II CATG 6 cut(s) 288, 446, 866, 1136, 1186, 1248
Kzo9I GATC 3 cut(s) 30, 705, 1221
LguI GCTCTTC 1 cut(s) 1235
LmnI GCTCC 4 cut(s) 286, 323, 333, 421
Lsp1109I GCAGC 1 cut(s) 1018
LweI GCATC 1 cut(s) 1231
MaeI CTAG 2 cut(s) 198, 911
MaeII ACGT 1 cut(s) 451
MaeIII GTNAC 1 cut(s) 801
MalI GATC 3 cut(s) 32, 707, 1223
MboI GATC 3 cut(s) 30, 705, 1221
MboII GAAGA 3 cut(s) 249, 1107, 1222
MlyI GAGTC 1 cut(s) 880
MmeI TCCRAC 1 cut(s) 92
MnlI CCTC 3 cut(s) 468, 475, 673
Mph1103I ATGCAT 3 cut(s) 290, 1246, 1250
MslI CAYNNNNRTG 2 cut(s) 289, 1249
MspI CCGG 1 cut(s) 566
MspR9I CCNGG 3 cut(s) 477, 1077, 1141
Mva1269I GAATGC 1 cut(s) 1033
MvaI CCWGG 3 cut(s) 477, 1077, 1141
MwoI GCNNNNNNNGC 2 cut(s) 354, 1129
NdeI CATATG 3 cut(s) 290, 360, 1250
NdeII GATC 3 cut(s) 30, 705, 1221
NlaIII CATG 6 cut(s) 288, 446, 866, 1136, 1186, 1248
NlaIV GGNNCC 2 cut(s) 329, 1138
NsiI ATGCAT 3 cut(s) 290, 1246, 1250
NspI RCATGY 1 cut(s) 1248
NspV TTCGAA 1 cut(s) 977
PaeI GCATGC 1 cut(s) 1248
PceI AGGCCT 1 cut(s) 475
PciSI GCTCTTC 1 cut(s) 1235
PctI GAATGC 1 cut(s) 1033
PfeI GAWTC 5 cut(s) 184, 202, 220, 238, 1108
PkrI GCNGC 1 cut(s) 1033
PleI GAGTC 1 cut(s) 880
PpsI GAGTC 1 cut(s) 880
Psp6I CCWGG 3 cut(s) 475, 1075, 1139
PspFI CCCAGC 2 cut(s) 551, 1034
PspGI CCWGG 3 cut(s) 475, 1075, 1139
PspN4I GGNNCC 2 cut(s) 329, 1138
PspPI GGNCC 3 cut(s) 364, 1037, 1079
PstNI CAGNNNCTG 1 cut(s) 1129
RsaI GTAC 3 cut(s) 447, 486, 1015
RsaNI GTAC 3 cut(s) 446, 485, 1014
RseI CAYNNNNRTG 2 cut(s) 289, 1249
SalI GTCGAC 1 cut(s) 883
SapI GCTCTTC 1 cut(s) 1235
SatI GCNGC 1 cut(s) 1032
Sau3AI GATC 3 cut(s) 30, 705, 1221
Sau96I GGNCC 3 cut(s) 364, 1037, 1079
SchI GAGTC 1 cut(s) 880
ScrFI CCNGG 3 cut(s) 477, 1077, 1141
SfaNI GCATC 1 cut(s) 1231
SfcI CTRYAG 2 cut(s) 430, 1065
SfuI TTCGAA 1 cut(s) 977
SinI GGWCC 1 cut(s) 364
SmiMI CAYNNNNRTG 2 cut(s) 289, 1249
SphI GCATGC 1 cut(s) 1248
SseBI AGGCCT 1 cut(s) 475
SspI AATATT 2 cut(s) 770, 1200
SspMI CTAG 2 cut(s) 198, 911
StuI AGGCCT 1 cut(s) 475
StyD4I CCNGG 3 cut(s) 475, 1075, 1139
StyI CCWWGG 1 cut(s) 155
TaaI ACNGT 3 cut(s) 434, 510, 604
TaiI ACGT 1 cut(s) 454
TaqI TCGA 5 cut(s) 850, 884, 918, 925, 977
TatI WGTACW 2 cut(s) 445, 1013
TfiI GAWTC 5 cut(s) 184, 202, 220, 238, 1108
TscAI CASTG 1 cut(s) 1107
TseI GCWGC 1 cut(s) 1031
TspDTI ATGAA 3 cut(s) 426, 851, 862
TspRI CASTG 1 cut(s) 1107
VpaK11BI GGWCC 1 cut(s) 364
XagI CCTNNNNNAGG 1 cut(s) 469
XapI RAATTY 5 cut(s) 712, 829, 869, 979, 1145
XceI RCATGY 1 cut(s) 1248
XmiI GTMKAC 1 cut(s) 884
XspI CTAG 2 cut(s) 198, 911
Zsp2I ATGCAT 3 cut(s) 290, 1246, 1250
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.