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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AciI | CCGC | 1 cut(s) | 9 |
| AclI | AACGTT | 1 cut(s) | 389 |
| AclWI | GGATC | 2 cut(s) | 87, 363 |
| AcsI | RAATTY | 3 cut(s) | 34, 236, 534 |
| AgsI | TTSAA | 3 cut(s) | 285, 424, 447 |
| AjuI | GAANNNNNNNTTGG | 2 cut(s) | 247, 279 |
| AlwI | GGATC | 2 cut(s) | 87, 363 |
| ApoI | RAATTY | 3 cut(s) | 34, 236, 534 |
| AspS9I | GGNCC | 3 cut(s) | 89, 365, 572 |
| AsuC2I | CCSGG | 2 cut(s) | 93, 369 |
| AvaII | GGWCC | 3 cut(s) | 89, 365, 572 |
| BccI | CCATC | 2 cut(s) | 547, 551 |
| BciVI | GTATCC | 1 cut(s) | 253 |
| BcnI | CCSGG | 2 cut(s) | 93, 369 |
| BfaI | CTAG | 3 cut(s) | 15, 23, 516 |
| BfmI | CTRYAG | 2 cut(s) | 40, 486 |
| BfuI | GTATCC | 1 cut(s) | 253 |
| BisI | GCNGC | 1 cut(s) | 9 |
| BlsI | GCNGC | 1 cut(s) | 10 |
| Bme1390I | CCNGG | 2 cut(s) | 93, 369 |
| Bme18I | GGWCC | 3 cut(s) | 89, 365, 572 |
| BmgT120I | GGNCC | 3 cut(s) | 89, 365, 572 |
| BmrFI | CCNGG | 2 cut(s) | 93, 369 |
| BmsI | GCATC | 4 cut(s) | 318, 334, 628, 654 |
| BplI | GAGNNNNNCTC | 2 cut(s) | 94, 126 |
| BpuMI | CCSGG | 2 cut(s) | 93, 369 |
| Bsa29I | ATCGAT | 1 cut(s) | 608 |
| BsaJI | CCNNGG | 2 cut(s) | 254, 368 |
| Bse1I | ACTGG | 1 cut(s) | 303 |
| BseCI | ATCGAT | 1 cut(s) | 608 |
| BseDI | CCNNGG | 2 cut(s) | 254, 368 |
| BseGI | GGATG | 2 cut(s) | 349, 562 |
| BseNI | ACTGG | 1 cut(s) | 303 |
| BshVI | ATCGAT | 1 cut(s) | 608 |
| BsiSI | CCGG | 2 cut(s) | 92, 368 |
| Bsp143I | GATC | 2 cut(s) | 79, 355 |
| BspACI | CCGC | 1 cut(s) | 9 |
| BspDI | ATCGAT | 1 cut(s) | 608 |
| BspPI | GGATC | 2 cut(s) | 87, 363 |
| BsrI | ACTGG | 1 cut(s) | 303 |
| BssECI | CCNNGG | 2 cut(s) | 254, 368 |
| BssMI | GATC | 2 cut(s) | 79, 355 |
| BssT1I | CCWWGG | 1 cut(s) | 254 |
| Bst4CI | ACNGT | 2 cut(s) | 439, 661 |
| BstF5I | GGATG | 2 cut(s) | 349, 562 |
| BstKTI | GATC | 2 cut(s) | 82, 358 |
| BstMBI | GATC | 2 cut(s) | 79, 355 |
| BstSCI | CCNGG | 2 cut(s) | 91, 367 |
| BstSFI | CTRYAG | 2 cut(s) | 40, 486 |
| Bsu15I | ATCGAT | 1 cut(s) | 608 |
| BsuI | GTATCC | 1 cut(s) | 253 |
| BsuTUI | ATCGAT | 1 cut(s) | 608 |
| BtsCI | GGATG | 2 cut(s) | 349, 562 |
| Cfr13I | GGNCC | 3 cut(s) | 89, 365, 572 |
| ClaI | ATCGAT | 1 cut(s) | 608 |
| CviAII | CATG | 1 cut(s) | 587 |
| CviJI | RGCY | 1 cut(s) | 18 |
| CviKI_1 | RGCY | 1 cut(s) | 18 |
| DpnI | GATC | 2 cut(s) | 81, 357 |
| DpnII | GATC | 2 cut(s) | 79, 355 |
| DraI | TTTAAA | 2 cut(s) | 241, 457 |
| Eco130I | CCWWGG | 1 cut(s) | 254 |
| Eco47I | GGWCC | 3 cut(s) | 89, 365, 572 |
| EcoT14I | CCWWGG | 1 cut(s) | 254 |
| ErhI | CCWWGG | 1 cut(s) | 254 |
| FaeI | CATG | 1 cut(s) | 590 |
| FaiI | YATR | 9 cut(s) | 42, 71, 291, 339, 488, 588, 601, 603, 636 |
| FalI | AAGNNNNNCTT | 2 cut(s) | 247, 279 |
| FatI | CATG | 1 cut(s) | 586 |
| Fnu4HI | GCNGC | 1 cut(s) | 9 |
| FokI | GGATG | 2 cut(s) | 356, 569 |
| Fsp4HI | GCNGC | 1 cut(s) | 9 |
| FspBI | CTAG | 3 cut(s) | 15, 23, 516 |
| GluI | GCNGC | 1 cut(s) | 9 |
| HapII | CCGG | 2 cut(s) | 92, 368 |
| Hin1II | CATG | 1 cut(s) | 590 |
| HincII | GTYRAC | 1 cut(s) | 657 |
| HindII | GTYRAC | 1 cut(s) | 657 |
| HpaI | GTTAAC | 1 cut(s) | 657 |
| HpaII | CCGG | 2 cut(s) | 92, 368 |
| Hpy166II | GTNNAC | 2 cut(s) | 393, 657 |
| Hpy188I | TCNGA | 1 cut(s) | 316 |
| Hpy188III | TCNNGA | 3 cut(s) | 23, 87, 363 |
| Hpy8I | GTNNAC | 2 cut(s) | 393, 657 |
| HpyAV | CCTTC | 1 cut(s) | 29 |
| HpyCH4III | ACNGT | 2 cut(s) | 439, 661 |
| HpyCH4IV | ACGT | 1 cut(s) | 389 |
| HpyCH4V | TGCA | 2 cut(s) | 347, 667 |
| HpySE526I | ACGT | 1 cut(s) | 389 |
| Hsp92II | CATG | 1 cut(s) | 590 |
| KspAI | GTTAAC | 1 cut(s) | 657 |
| Kzo9I | GATC | 2 cut(s) | 79, 355 |
| LpnPI | CCDG | 5 cut(s) | 72, 105, 316, 348, 381 |
| LweI | GCATC | 4 cut(s) | 318, 334, 628, 654 |
| MaeI | CTAG | 3 cut(s) | 15, 23, 516 |
| MaeII | ACGT | 1 cut(s) | 389 |
| MalI | GATC | 2 cut(s) | 81, 357 |
| MboI | GATC | 2 cut(s) | 79, 355 |
| MfeI | CAATTG | 1 cut(s) | 617 |
| MluCI | AATT | 7 cut(s) | 34, 96, 136, 236, 293, 534, 617 |
| MlyI | GAGTC | 7 cut(s) | 112, 158, 167, 177, 394, 403, 423 |
| MnlI | CCTC | 3 cut(s) | 96, 372, 563 |
| MseI | TTAA | 5 cut(s) | 135, 240, 383, 456, 656 |
| MspI | CCGG | 2 cut(s) | 92, 368 |
| MspR9I | CCNGG | 2 cut(s) | 93, 369 |
| MunI | CAATTG | 1 cut(s) | 617 |
| NciI | CCSGG | 2 cut(s) | 93, 369 |
| NdeII | GATC | 2 cut(s) | 79, 355 |
| NlaIII | CATG | 1 cut(s) | 590 |
| PfeI | GAWTC | 4 cut(s) | 107, 443, 475, 605 |
| PkrI | GCNGC | 1 cut(s) | 10 |
| PleI | GAGTC | 7 cut(s) | 112, 158, 167, 177, 394, 403, 423 |
| PpsI | GAGTC | 7 cut(s) | 112, 158, 167, 177, 394, 403, 423 |
| Psp1406I | AACGTT | 1 cut(s) | 389 |
| PspPI | GGNCC | 3 cut(s) | 89, 365, 572 |
| PsrI | GAACNNNNNNTAC | 4 cut(s) | 67, 99, 343, 375 |
| SaqAI | TTAA | 5 cut(s) | 135, 240, 383, 456, 656 |
| SatI | GCNGC | 1 cut(s) | 9 |
| Sau3AI | GATC | 2 cut(s) | 79, 355 |
| Sau96I | GGNCC | 3 cut(s) | 89, 365, 572 |
| SchI | GAGTC | 7 cut(s) | 112, 158, 167, 177, 394, 403, 423 |
| ScrFI | CCNGG | 2 cut(s) | 93, 369 |
| SetI | ASST | 4 cut(s) | 16, 383, 392, 574 |
| SfaNI | GCATC | 4 cut(s) | 318, 334, 628, 654 |
| SfcI | CTRYAG | 2 cut(s) | 40, 486 |
| SinI | GGWCC | 3 cut(s) | 89, 365, 572 |
| Sse9I | AATT | 7 cut(s) | 34, 96, 136, 236, 293, 534, 617 |
| SsiI | CCGC | 1 cut(s) | 9 |
| SspMI | CTAG | 3 cut(s) | 15, 23, 516 |
| StyD4I | CCNGG | 2 cut(s) | 91, 367 |
| StyI | CCWWGG | 1 cut(s) | 254 |
| TaaI | ACNGT | 2 cut(s) | 439, 661 |
| TaiI | ACGT | 1 cut(s) | 392 |
| TaqI | TCGA | 2 cut(s) | 412, 608 |
| TasI | AATT | 7 cut(s) | 34, 96, 136, 236, 293, 534, 617 |
| TauI | GCSGC | 1 cut(s) | 11 |
| TfiI | GAWTC | 4 cut(s) | 107, 443, 475, 605 |
| Tru1I | TTAA | 5 cut(s) | 135, 240, 383, 456, 656 |
| Tru9I | TTAA | 5 cut(s) | 135, 240, 383, 456, 656 |
| TspDTI | ATGAA | 1 cut(s) | 618 |
| VpaK11BI | GGWCC | 3 cut(s) | 89, 365, 572 |
| XapI | RAATTY | 3 cut(s) | 34, 236, 534 |
| XbaI | TCTAGA | 1 cut(s) | 22 |
| XspI | CTAG | 3 cut(s) | 15, 23, 516 |