pycom01g03610

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr1
OG0000608
Physical Location & Seq
Forward (+)
2727149 .. 2729728
2580 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom01g03610.10

Sequence Viewer

Length: 1815 bp
ATGAATATGACGCTAGAATATAAAAATTCTTGGCATAAGTTTCATGCAATCATAGTAACGAATGCCTCGTCAACACTTATAATTTTCATAGAGCATAATGATCTCTCAATGTACCTCTTCTATGCATTCATGTTAATTAGTGCATCACAGTTAACTTGGGGTGTTGAGAATTCATGGTTTATTGAAATGCAATTGGAAATCATTTTATTTTGCAAGTGTAACATTAAATCAAATTCGTTTTACAATCTGCTTAGTTTATTAACTTGTTTTGTTATTTCAATCTTCGTCAAATCAAAACCCCCCTTTACTTTCTTCATTTTGAGTCTTTGGTTTGTCTTAGTTGTTTTATATTGTGTTTTTACGTTTTTGAGTCAAATCCAAGTGATTAACAATCCCTCCCAATCCTCGGTCCAGAACGATCCTTACTTATACTTATACTACAATTGTCAAAAAGAGGGTTTAATTTCTGTGCGTATAATTCACGCATCATCTGCATGCTATTCAATAATAATGACAGGTCACTTAACACTGTTTTTGGAATCTAAGTCACTATTTGTGGAACCTAAGTTACTTTACTTCTTGACTCTAAGTCACTCTAGAAAAGAAATAATGAAATTCACTGTATTTGTATTTAAAAGACTGTTTCTGGAATCTAAGTCACTGTTTCTGGAATTTAAGTCACTGTTTCTGGATTTTGGTTCTTTTCTTTCTCGGTACAGTGTTTATTTGTTCCAGACAAAACAAACACTGTATCAAATTTTTTTTTTCCCAGATACATTCTTCTTTTTTTTCCAGACACTTTTTCACCAGTTTCTGCCATTAAACTTCTTTGAACCTGTGTTCGGCGGCTTTGTTCCGATGAATTCTTCTTTTTTCAACTTTGATTTGAACTTTGATTTGGTTGCCGGCAAGAGCCATGGTCACGTTAATTCTCGAGCCCATAAAATCAATCCACACTTGATAGTTAATTCCATTGTTGAGCTTCAACTCGTTGAAATCTTGATCAAGCCCACCACTCCAATACCCAGTGGTGCTAGAAGCATTAGACGTAAGGGAGTTGATGTCCAAACCAACATGGTTGTACCATCAGGATCCTGGAAATCTTGGTTTTCGAAGACATCAAACTCGACACCGAAGACATTGTTGTTGGGGTTGCCGTTTTCGTTTTTATTTATTTTAATCGAGGGAATTGACGAAAATGCCCGAGAGGCAAGGATTTTGACTTTTGTTGACCATCGTCTAGAGGAACGTATGACTTATTCTTTTGGCCCGTTCACAAGTCCAGATTATAATGATTTAAAGGAAAATAAACTCCCACCATGTGGGAATTGTAACCAATTAGAGAAACAGGAAAGGAAGAAGCAACCAATCAGAAAAAGGGGGGAAAGGAAAGGGAAAAAAAAAGAAAGAAAGAAGAAGGAAAAAAGCCCAGCTTCCCGTGCACCAGCCCGACTCCATTTTCGGCGGTTTTTGCCATTCTTTCCATCGATTCATCACCATTCATCGCCTACAACCTCTTCCTCAACCCTCCCTCTTCTATCTCCACCCAAATTTGAGGATTTTAAGGTCCAATTCGTTTCTCCAATCCGGCAAACCCAAGAACAAGACCCAACTGGTTATAGGGGCATTGAAGCACCGAGGAAGTCGAATCGAAGAATACCCACCTTAGGGTTTCCTGCAGGTTCAAATCAATTTGGAGCCTTTCCTGGCCAAATTGGACTTGGCCACGAATTTTTAGAGATAGTTGAATTTTCCGGTGAATTGGGGCGGCTAATGACGTGTGCGTGGCGGCGCGTGAGCCGAGTCACCCCCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

605

Amino Acids

69.96

Weight (kDa)

9.59

Isoelectric Point (pI)

41.68

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 80, 1290
Acc36I ACCTGC 1 cut(s) 1670
AccB7I CCANNNNNTGG 1 cut(s) 1322
AccII CGCG 1 cut(s) 1794
AciI CCGC 4 cut(s) 846, 1465, 1768, 1789
AclWI GGATC 3 cut(s) 413, 1086, 1099
AcoI YGGCCR 2 cut(s) 1708, 1723
AdeI CACNNNGTG 1 cut(s) 1322
AfaI GTAC 3 cut(s) 113, 716, 1083
AfiI CCNNNNNNNGG 5 cut(s) 406, 842, 1322, 1667, 1668
AflIII ACRYGT 1 cut(s) 1778
AhdI GACNNNNNGTC 1 cut(s) 588
AjiI CACGTC 1 cut(s) 1779
AjnI CCWGG 2 cut(s) 1094, 1705
AjuI GAANNNNNNNTTGG 2 cut(s) 881, 913
AluBI AGCT 2 cut(s) 982, 1433
AluI AGCT 2 cut(s) 982, 1433
Alw21I GWGCWC 1 cut(s) 1444
Alw44I GTGCAC 1 cut(s) 1440
AlwI GGATC 3 cut(s) 413, 1086, 1099
AlwNI CAGNNNCTG 1 cut(s) 814
Ama87I CYCGRG 2 cut(s) 933, 1203
AoxI GGCC 3 cut(s) 1267, 1708, 1723
ApaLI GTGCAC 1 cut(s) 1440
ArsI GACNNNNNNTTYG 4 cut(s) 53, 85, 1443, 1475
AspLEI GCGC 1 cut(s) 1794
AspS9I GGNCC 3 cut(s) 409, 1268, 1567
AsuHPI GGTGA 4 cut(s) 797, 1487, 1769, 1798
AsuII TTCGAA 1 cut(s) 1112
AvaI CYCGRG 2 cut(s) 933, 1203
AvaII GGWCC 2 cut(s) 409, 1567
AxyI CCTNAGG 1 cut(s) 1666
BaeGI GKGCMC 1 cut(s) 1444
BalI TGGCCA 2 cut(s) 1710, 1725
BamHI GGATCC 1 cut(s) 1091
BanII GRGCYC 1 cut(s) 940
BbsI GAAGAC 2 cut(s) 1121, 1142
Bbv12I GWGCWC 1 cut(s) 1444
BccI CCATC 3 cut(s) 1093, 1242, 1492
BceAI ACGGC 1 cut(s) 1141
BciT130I CCWGG 2 cut(s) 1096, 1707
BclI TGATCA 1 cut(s) 1002
BfaI CTAG 4 cut(s) 14, 597, 1035, 1241
BfmI CTRYAG 1 cut(s) 1677
BfuAI ACCTGC 1 cut(s) 1670
BglI GCCNNNNNGGC 1 cut(s) 1208
BisI GCNGC 3 cut(s) 847, 1769, 1790
BlsI GCNGC 3 cut(s) 848, 1770, 1791
Bme1390I CCNGG 2 cut(s) 1096, 1707
Bme18I GGWCC 2 cut(s) 409, 1567
BmeRI GACNNNNNGTC 1 cut(s) 588
BmeT110I CYCGRG 2 cut(s) 933, 1203
BmgBI CACGTC 1 cut(s) 1779
BmgT120I GGNCC 3 cut(s) 409, 1268, 1567
BmiI GGNNCC 3 cut(s) 561, 1093, 1699
BmrFI CCNGG 2 cut(s) 1096, 1707
BmrI ACTGGG 1 cut(s) 1020
BmsI GCATC 2 cut(s) 152, 494
BmuI ACTGGG 1 cut(s) 1020
BoxI GACNNNNGTC 1 cut(s) 1236
BpiI GAAGAC 2 cut(s) 1121, 1142
Bpu14I TTCGAA 1 cut(s) 1112
Bsa29I ATCGAT 1 cut(s) 1487
BsaJI CCNNGG 3 cut(s) 405, 916, 1637
BsaWI WCCGGW 1 cut(s) 1754
BsaXI ACNNNNNCTCC 4 cut(s) 380, 410, 1047, 1077
Bsc4I CCNNNNNNNGG 5 cut(s) 406, 842, 1322, 1667, 1668
Bse118I RCCGGY 1 cut(s) 905
Bse1I ACTGG 3 cut(s) 808, 1026, 1618
Bse21I CCTNAGG 1 cut(s) 1666
BseBI CCWGG 2 cut(s) 1096, 1707
BseCI ATCGAT 1 cut(s) 1487
BseDI CCNNGG 3 cut(s) 405, 916, 1637
BseLI CCNNNNNNNGG 5 cut(s) 406, 842, 1322, 1667, 1668
BseNI ACTGG 3 cut(s) 808, 1026, 1618
BseSI GKGCMC 1 cut(s) 1444
BseYI CCCAGC 1 cut(s) 1429
Bsh1236I CGCG 1 cut(s) 1794
BshFI GGCC 3 cut(s) 1269, 1710, 1725
BshVI ATCGAT 1 cut(s) 1487
BsiHKAI GWGCWC 1 cut(s) 1444
BsiHKCI CYCGRG 2 cut(s) 933, 1203
BsiSI CCGG 3 cut(s) 906, 1588, 1755
BslI CCNNNNNNNGG 5 cut(s) 406, 842, 1322, 1667, 1668
BsmI GAATGC 2 cut(s) 67, 125
BsnI GGCC 3 cut(s) 1269, 1710, 1725
BsoBI CYCGRG 2 cut(s) 933, 1203
Bsp119I TTCGAA 1 cut(s) 1112
Bsp1286I GDGCHC 2 cut(s) 940, 1444
Bsp143I GATC 4 cut(s) 100, 418, 1002, 1091
Bsp19I CCATGG 1 cut(s) 916
BspACI CCGC 4 cut(s) 846, 1465, 1768, 1789
BspANI GGCC 3 cut(s) 1269, 1710, 1725
BspDI ATCGAT 1 cut(s) 1487
BspFNI CGCG 1 cut(s) 1794
BspLI GGNNCC 3 cut(s) 561, 1093, 1699
BspMAI CTGCAG 1 cut(s) 1681
BspMI ACCTGC 1 cut(s) 1670
BspPI GGATC 3 cut(s) 413, 1086, 1099
BspT104I TTCGAA 1 cut(s) 1112
BsrFI RCCGGY 1 cut(s) 905
BsrI ACTGG 3 cut(s) 808, 1026, 1618
BssAI RCCGGY 1 cut(s) 905
BssECI CCNNGG 3 cut(s) 405, 916, 1637
BssMI GATC 4 cut(s) 100, 418, 1002, 1091
BssT1I CCWWGG 1 cut(s) 916
Bst2UI CCWGG 2 cut(s) 1096, 1707
Bst4CI ACNGT 8 cut(s) 150, 531, 622, 642, 663, 684, 719, 750
Bst6I CTCTTC 3 cut(s) 122, 1522, 1539
BstAPI GCANNNNNTGC 1 cut(s) 491
BstBI TTCGAA 1 cut(s) 1112
BstC8I GCNNGC 2 cut(s) 496, 907
BstDEI CTNAG 7 cut(s) 251, 337, 543, 564, 587, 654, 1666
BstDSI CCRYGG 1 cut(s) 916
BstFNI CGCG 1 cut(s) 1794
BstHHI GCGC 1 cut(s) 1794
BstKTI GATC 4 cut(s) 103, 421, 1005, 1094
BstMBI GATC 4 cut(s) 100, 418, 1002, 1091
BstMWI GCNNNNNNNGC 4 cut(s) 491, 1208, 1439, 1471
BstNI CCWGG 2 cut(s) 1096, 1707
BstNSI RCATGY 1 cut(s) 498
BstPAI GACNNNNGTC 1 cut(s) 1236
BstSCI CCNGG 2 cut(s) 1094, 1705
BstSFI CTRYAG 1 cut(s) 1677
BstSLI GKGCMC 1 cut(s) 1444
BstUI CGCG 1 cut(s) 1794
BstV2I GAAGAC 2 cut(s) 1121, 1142
BstX2I RGATCY 1 cut(s) 1091
BstYI RGATCY 1 cut(s) 1091
Bsu15I ATCGAT 1 cut(s) 1487
Bsu36I CCTNAGG 1 cut(s) 1666
BsuRI GGCC 3 cut(s) 1269, 1710, 1725
BsuTUI ATCGAT 1 cut(s) 1487
BtgI CCRYGG 1 cut(s) 916
BtgZI GCGATG 1 cut(s) 1488
BtrI CACGTC 1 cut(s) 1779
BtsIMutI CAGTG 7 cut(s) 527, 618, 659, 680, 724, 746, 1033
BveI ACCTGC 1 cut(s) 1670
Cac8I GCNNGC 2 cut(s) 496, 907
CaiI CAGNNNCTG 1 cut(s) 814
CfoI GCGC 1 cut(s) 1794
Cfr10I RCCGGY 1 cut(s) 905
Cfr13I GGNCC 3 cut(s) 409, 1268, 1567
ClaI ATCGAT 1 cut(s) 1487
CseI GACGC 1 cut(s) 19
Csp6I GTAC 3 cut(s) 112, 715, 1082
CviAII CATG 7 cut(s) 44, 130, 174, 495, 917, 1075, 1320
CviQI GTAC 3 cut(s) 112, 715, 1082
DdeI CTNAG 7 cut(s) 251, 337, 543, 564, 587, 654, 1666
DpnI GATC 4 cut(s) 102, 420, 1004, 1093
DpnII GATC 4 cut(s) 100, 418, 1002, 1091
DraI TTTAAA 2 cut(s) 634, 1299
DraIII CACNNNGTG 1 cut(s) 1322
DriI GACNNNNNGTC 1 cut(s) 588
EaeI YGGCCR 2 cut(s) 1708, 1723
Eam1104I CTCTTC 3 cut(s) 122, 1522, 1539
Eam1105I GACNNNNNGTC 1 cut(s) 588
EarI CTCTTC 3 cut(s) 122, 1522, 1539
Eco130I CCWWGG 1 cut(s) 916
Eco24I GRGCYC 1 cut(s) 940
Eco47I GGWCC 2 cut(s) 409, 1567
Eco81I CCTNAGG 1 cut(s) 1666
Eco88I CYCGRG 2 cut(s) 933, 1203
EcoRI GAATTC 2 cut(s) 169, 862
EcoRII CCWGG 2 cut(s) 1094, 1705
EcoT14I CCWWGG 1 cut(s) 916
EcoT22I ATGCAT 1 cut(s) 127
EcoT38I GRGCYC 1 cut(s) 940
ErhI CCWWGG 1 cut(s) 916
FaeI CATG 7 cut(s) 47, 133, 177, 498, 920, 1078, 1323
FalI AAGNNNNNCTT 2 cut(s) 1417, 1449
FatI CATG 7 cut(s) 43, 129, 173, 494, 916, 1074, 1319
FbaI TGATCA 1 cut(s) 1002
Fnu4HI GCNGC 3 cut(s) 847, 1769, 1790
FriOI GRGCYC 1 cut(s) 940
Fsp4HI GCNGC 3 cut(s) 847, 1769, 1790
FspBI CTAG 4 cut(s) 14, 597, 1035, 1241
GlaI GCGC 1 cut(s) 1793
GluI GCNGC 3 cut(s) 847, 1769, 1790
GsaI CCCAGC 1 cut(s) 1433
HaeIII GGCC 3 cut(s) 1269, 1710, 1725
HapII CCGG 3 cut(s) 906, 1588, 1755
HgaI GACGC 1 cut(s) 19
HhaI GCGC 1 cut(s) 1794
Hin1II CATG 7 cut(s) 47, 133, 177, 498, 920, 1078, 1323
Hin6I GCGC 1 cut(s) 1792
HinP1I GCGC 1 cut(s) 1792
HincII GTYRAC 3 cut(s) 72, 153, 1231
HindII GTYRAC 3 cut(s) 72, 153, 1231
HinfI GANTC 9 cut(s) 322, 370, 539, 583, 650, 1452, 1489, 1648, 1803
HpaI GTTAAC 1 cut(s) 153
HpaII CCGG 3 cut(s) 906, 1588, 1755
HphI GGTGA 4 cut(s) 797, 1487, 1769, 1798
Hpy166II GTNNAC 5 cut(s) 72, 153, 1231, 1275, 1442
Hpy188I TCNGA 2 cut(s) 858, 1373
Hpy8I GTNNAC 5 cut(s) 72, 153, 1231, 1275, 1442
HpyAV CCTTC 1 cut(s) 1411
HpyCH4III ACNGT 8 cut(s) 150, 531, 622, 642, 663, 684, 719, 750
HpyCH4IV ACGT 5 cut(s) 362, 924, 1048, 1249, 1778
HpyCH4V TGCA 8 cut(s) 47, 125, 143, 190, 213, 494, 1442, 1679
HpyF10VI GCNNNNNNNGC 4 cut(s) 491, 1208, 1439, 1471
HpyF3I CTNAG 7 cut(s) 251, 337, 543, 564, 587, 654, 1666
HpySE526I ACGT 5 cut(s) 362, 924, 1048, 1249, 1778
Hsp92II CATG 7 cut(s) 47, 133, 177, 498, 920, 1078, 1323
HspAI GCGC 1 cut(s) 1792
KroI GCCGGC 1 cut(s) 905
KroNI GCCGGC 1 cut(s) 907
Ksp22I TGATCA 1 cut(s) 1002
KspAI GTTAAC 1 cut(s) 153
Kzo9I GATC 4 cut(s) 100, 418, 1002, 1091
LmnI GCTCC 1 cut(s) 1697
LweI GCATC 2 cut(s) 152, 494
MaeI CTAG 4 cut(s) 14, 597, 1035, 1241
MaeII ACGT 5 cut(s) 362, 924, 1048, 1249, 1778
MalI GATC 4 cut(s) 102, 420, 1004, 1093
MboI GATC 4 cut(s) 100, 418, 1002, 1091
MfeI CAATTG 2 cut(s) 191, 442
MflI RGATCY 1 cut(s) 1091
MhlI GDGCHC 2 cut(s) 940, 1444
MlsI TGGCCA 2 cut(s) 1710, 1725
MluNI TGGCCA 2 cut(s) 1710, 1725
MlyI GAGTC 5 cut(s) 331, 379, 577, 1446, 1812
Mox20I TGGCCA 2 cut(s) 1710, 1725
Mph1103I ATGCAT 1 cut(s) 127
MroNI GCCGGC 1 cut(s) 905
MscI TGGCCA 2 cut(s) 1710, 1725
MslI CAYNNNNRTG 1 cut(s) 493
Msp20I TGGCCA 2 cut(s) 1710, 1725
MspI CCGG 3 cut(s) 906, 1588, 1755
MspR9I CCNGG 2 cut(s) 1096, 1707
MunI CAATTG 2 cut(s) 191, 442
Mva1269I GAATGC 2 cut(s) 67, 125
MvaI CCWGG 2 cut(s) 1096, 1707
MvnI CGCG 1 cut(s) 1794
MwoI GCNNNNNNNGC 4 cut(s) 491, 1208, 1439, 1471
NaeI GCCGGC 1 cut(s) 907
NcoI CCATGG 1 cut(s) 916
NdeII GATC 4 cut(s) 100, 418, 1002, 1091
NgoMIV GCCGGC 1 cut(s) 905
NlaIII CATG 7 cut(s) 47, 133, 177, 498, 920, 1078, 1323
NlaIV GGNNCC 3 cut(s) 561, 1093, 1699
NmuCI GTSAC 7 cut(s) 518, 546, 590, 657, 678, 920, 1804
NsiI ATGCAT 1 cut(s) 127
NspI RCATGY 1 cut(s) 498
NspV TTCGAA 1 cut(s) 1112
PaeI GCATGC 1 cut(s) 498
PaeR7I CTCGAG 1 cut(s) 933
PcsI WCGNNNNNNNCGW 1 cut(s) 65
PctI GAATGC 2 cut(s) 67, 125
PdiI GCCGGC 1 cut(s) 907
PfeI GAWTC 4 cut(s) 539, 650, 1489, 1648
PflMI CCANNNNNTGG 1 cut(s) 1322
PfoI TCCNGGA 1 cut(s) 1094
PkrI GCNGC 3 cut(s) 848, 1770, 1791
PleI GAGTC 5 cut(s) 330, 378, 577, 1446, 1811
PpsI GAGTC 5 cut(s) 330, 378, 577, 1446, 1811
PshAI GACNNNNGTC 1 cut(s) 1236
PsiI TTATAA 2 cut(s) 80, 1290
Psp6I CCWGG 2 cut(s) 1094, 1705
PspFI CCCAGC 1 cut(s) 1429
PspGI CCWGG 2 cut(s) 1094, 1705
PspN4I GGNNCC 3 cut(s) 561, 1093, 1699
PspPI GGNCC 3 cut(s) 409, 1268, 1567
PsrI GAACNNNNNNTAC 4 cut(s) 407, 439, 552, 584
PstI CTGCAG 1 cut(s) 1681
PstNI CAGNNNCTG 1 cut(s) 814
PsuI RGATCY 1 cut(s) 1091
RsaI GTAC 3 cut(s) 113, 716, 1083
RsaNI GTAC 3 cut(s) 112, 715, 1082
RseI CAYNNNNRTG 1 cut(s) 493
SatI GCNGC 3 cut(s) 847, 1769, 1790
Sau3AI GATC 4 cut(s) 100, 418, 1002, 1091
Sau96I GGNCC 3 cut(s) 409, 1268, 1567
SbfI CCTGCAGG 1 cut(s) 1681
SchI GAGTC 5 cut(s) 331, 379, 577, 1446, 1812
ScrFI CCNGG 2 cut(s) 1096, 1707
SdaI CCTGCAGG 1 cut(s) 1681
SduI GDGCHC 2 cut(s) 940, 1444
SfaNI GCATC 2 cut(s) 152, 494
SfcI CTRYAG 1 cut(s) 1677
Sfr274I CTCGAG 1 cut(s) 933
SfuI TTCGAA 1 cut(s) 1112
SinI GGWCC 2 cut(s) 409, 1567
SlaI CTCGAG 1 cut(s) 933
SmiMI CAYNNNNRTG 1 cut(s) 493
SmlI CTYRAG 1 cut(s) 933
SmoI CTYRAG 1 cut(s) 933
SphI GCATGC 1 cut(s) 498
Sse8387I CCTGCAGG 1 cut(s) 1681
SsiI CCGC 4 cut(s) 846, 1465, 1768, 1789
SspMI CTAG 4 cut(s) 14, 597, 1035, 1241
StyD4I CCNGG 2 cut(s) 1094, 1705
StyI CCWWGG 1 cut(s) 916
TaaI ACNGT 8 cut(s) 150, 531, 622, 642, 663, 684, 719, 750
TaiI ACGT 5 cut(s) 365, 927, 1051, 1252, 1781
TaqI TCGA 7 cut(s) 934, 1112, 1127, 1182, 1487, 1646, 1651
TaqII GACCGA 1 cut(s) 397
TauI GCSGC 3 cut(s) 849, 1771, 1792
TfiI GAWTC 4 cut(s) 539, 650, 1489, 1648
TscAI CASTG 7 cut(s) 534, 625, 666, 687, 724, 753, 1033
TseFI GTSAC 7 cut(s) 518, 546, 590, 657, 678, 920, 1804
Tsp45I GTSAC 7 cut(s) 518, 546, 590, 657, 678, 920, 1804
TspRI CASTG 7 cut(s) 534, 625, 666, 687, 724, 753, 1033
Van91I CCANNNNNTGG 1 cut(s) 1322
VneI GTGCAC 1 cut(s) 1440
VpaK11BI GGWCC 2 cut(s) 409, 1567
XbaI TCTAGA 2 cut(s) 596, 1240
XceI RCATGY 1 cut(s) 498
XcmI CCANNNNNNNNNTGG 2 cut(s) 1092, 1718
XhoI CTCGAG 1 cut(s) 933
XspI CTAG 4 cut(s) 14, 597, 1035, 1241
Zsp2I ATGCAT 1 cut(s) 127
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.