pycom01g02240

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr1
OG0000608
Physical Location & Seq
Forward (+)
1851334 .. 1852106
773 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom01g02240.1

Sequence Viewer

Length: 549 bp
ATGCATAACAAAGATGCATATCAAAAGTCATTAAGTTTTGTGAAAACTATAAGCATTGACAAGGCATTCATCACTATGAAACACATGAGACTCATACCAAGGATTTACTTAACACGATATCATGCATGCAAATTAAGTGTTAACCCCCAACCCACATACATAAACCATCATATAAAACATATAATCATGGCTTCGAATTCACCTCCAACTAAAAAGAAGTTAGTTCCTCATGTTCATAGAATACACCTAGAAACACTCCAGGTGGCTCGTCCTTCTTTCCATGCTGCGCACAATGGGGATTTGTGTGAATTTAGGCTCTTGAGGTGTGGTGGATGTGTTGTGGCATCATGTATTTATAGGATGAAGGGGAGGCACGAAATTGGGCTGAAAAGGAGAGGAATTAGGACCCCAAATCACCAAAGAACAAGGACACTTTCAATTAGATTCCAAGGAGGACAAGGAATGGTCCTTGTGGCAGATTCTAGAACTTCTAGAAGGGTTACATGTGGCATAAACTTAATGCACGAAAATAGGGCTTCTAGAACTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

183

Amino Acids

20.94

Weight (kDa)

10.97

Isoelectric Point (pI)

48.72

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 288
AcsI RAATTY 2 cut(s) 196, 308
AflIII ACRYGT 1 cut(s) 503
AgsI TTSAA 1 cut(s) 438
AjnI CCWGG 1 cut(s) 258
Alw26I GTCTC 1 cut(s) 82
ApeKI GCWGC 1 cut(s) 284
ApoI RAATTY 2 cut(s) 196, 308
AspLEI GCGC 1 cut(s) 289
AspS9I GGNCC 2 cut(s) 405, 466
AsuHPI GGTGA 2 cut(s) 192, 407
AsuII TTCGAA 1 cut(s) 194
AvaII GGWCC 2 cut(s) 405, 466
BbvI GCAGC 1 cut(s) 271
BccI CCATC 1 cut(s) 174
BciT130I CCWGG 1 cut(s) 260
BcoDI GTCTC 1 cut(s) 82
BfaI CTAG 4 cut(s) 248, 483, 492, 540
BisI GCNGC 1 cut(s) 285
BlsI GCNGC 1 cut(s) 286
Bme1390I CCNGG 1 cut(s) 260
Bme18I GGWCC 2 cut(s) 405, 466
BmgT120I GGNCC 2 cut(s) 405, 466
BmiI GGNNCC 1 cut(s) 407
BmrFI CCNGG 1 cut(s) 260
BmsI GCATC 2 cut(s) 4, 353
BpmI CTGGAG 1 cut(s) 242
Bpu14I TTCGAA 1 cut(s) 194
BpuEI CTTGAG 1 cut(s) 340
BsaBI GATNNNNATC 1 cut(s) 18
BsaJI CCNNGG 2 cut(s) 98, 448
Bse8I GATNNNNATC 1 cut(s) 18
BseBI CCWGG 1 cut(s) 260
BseDI CCNNGG 2 cut(s) 98, 448
BseGI GGATG 2 cut(s) 338, 366
BseJI GATNNNNATC 1 cut(s) 18
BseXI GCAGC 1 cut(s) 271
BsmAI GTCTC 1 cut(s) 82
BsmI GAATGC 1 cut(s) 65
Bsp119I TTCGAA 1 cut(s) 194
BspLI GGNNCC 1 cut(s) 407
BspT104I TTCGAA 1 cut(s) 194
BssECI CCNNGG 2 cut(s) 98, 448
BssT1I CCWWGG 2 cut(s) 98, 448
Bst2UI CCWGG 1 cut(s) 260
BstBI TTCGAA 1 cut(s) 194
BstC8I GCNNGC 1 cut(s) 127
BstF5I GGATG 2 cut(s) 338, 366
BstHHI GCGC 1 cut(s) 289
BstMAI GTCTC 1 cut(s) 82
BstNI CCWGG 1 cut(s) 260
BstNSI RCATGY 2 cut(s) 129, 507
BstSCI CCNGG 1 cut(s) 258
BstV1I GCAGC 1 cut(s) 271
BtsCI GGATG 2 cut(s) 338, 366
Cac8I GCNNGC 1 cut(s) 127
CfoI GCGC 1 cut(s) 289
Cfr13I GGNCC 2 cut(s) 405, 466
CviAII CATG 8 cut(s) 85, 122, 126, 187, 230, 281, 348, 504
CviJI RGCY 5 cut(s) 191, 266, 316, 385, 536
CviKI_1 RGCY 5 cut(s) 191, 266, 316, 385, 536
Eco130I CCWWGG 2 cut(s) 98, 448
Eco32I GATATC 1 cut(s) 119
Eco47I GGWCC 2 cut(s) 405, 466
EcoO109I RGGNCCY 1 cut(s) 405
EcoRI GAATTC 1 cut(s) 196
EcoRII CCWGG 1 cut(s) 258
EcoRV GATATC 1 cut(s) 119
EcoT14I CCWWGG 2 cut(s) 98, 448
EcoT22I ATGCAT 3 cut(s) 6, 19, 127
ErhI CCWWGG 2 cut(s) 98, 448
FaeI CATG 8 cut(s) 88, 125, 129, 190, 233, 284, 351, 507
FatI CATG 8 cut(s) 84, 121, 125, 186, 229, 280, 347, 503
Fnu4HI GCNGC 1 cut(s) 285
FokI GGATG 2 cut(s) 345, 373
Fsp4HI GCNGC 1 cut(s) 285
FspBI CTAG 4 cut(s) 248, 483, 492, 540
FspI TGCGCA 1 cut(s) 288
GlaI GCGC 1 cut(s) 288
GluI GCNGC 1 cut(s) 285
GsuI CTGGAG 1 cut(s) 242
HhaI GCGC 1 cut(s) 289
Hin1II CATG 8 cut(s) 88, 125, 129, 190, 233, 284, 351, 507
Hin6I GCGC 1 cut(s) 287
HinP1I GCGC 1 cut(s) 287
HincII GTYRAC 1 cut(s) 142
HindII GTYRAC 1 cut(s) 142
HinfI GANTC 3 cut(s) 90, 444, 479
HpaI GTTAAC 1 cut(s) 142
HphI GGTGA 2 cut(s) 192, 407
Hpy166II GTNNAC 1 cut(s) 142
Hpy188III TCNNGA 4 cut(s) 319, 483, 492, 540
Hpy8I GTNNAC 1 cut(s) 142
HpyAV CCTTC 3 cut(s) 282, 358, 489
HpyCH4V TGCA 5 cut(s) 4, 17, 125, 129, 523
Hsp92II CATG 8 cut(s) 88, 125, 129, 190, 233, 284, 351, 507
HspAI GCGC 1 cut(s) 287
KspAI GTTAAC 1 cut(s) 142
LpnPI CCDG 2 cut(s) 245, 272
Lsp1109I GCAGC 1 cut(s) 271
LweI GCATC 2 cut(s) 4, 353
MaeI CTAG 4 cut(s) 248, 483, 492, 540
MaeIII GTNAC 1 cut(s) 499
MluCI AATT 6 cut(s) 131, 196, 308, 378, 399, 438
MlyI GAGTC 1 cut(s) 84
MmeI TCCRAC 1 cut(s) 230
MnlI CCTC 6 cut(s) 213, 237, 315, 363, 389, 446
Mph1103I ATGCAT 3 cut(s) 6, 19, 127
MseI TTAA 5 cut(s) 32, 110, 134, 141, 518
MslI CAYNNNNRTG 1 cut(s) 74
MspR9I CCNGG 1 cut(s) 260
Mva1269I GAATGC 1 cut(s) 65
MvaI CCWGG 1 cut(s) 260
NlaIII CATG 8 cut(s) 88, 125, 129, 190, 233, 284, 351, 507
NlaIV GGNNCC 1 cut(s) 407
NsbI TGCGCA 1 cut(s) 288
NsiI ATGCAT 3 cut(s) 6, 19, 127
NspI RCATGY 2 cut(s) 129, 507
NspV TTCGAA 1 cut(s) 194
PaeI GCATGC 1 cut(s) 129
PciI ACATGT 1 cut(s) 503
PctI GAATGC 1 cut(s) 65
PfeI GAWTC 2 cut(s) 444, 479
PkrI GCNGC 1 cut(s) 286
PleI GAGTC 1 cut(s) 84
PpsI GAGTC 1 cut(s) 84
PpuMI RGGWCCY 1 cut(s) 405
PscI ACATGT 1 cut(s) 503
Psp5II RGGWCCY 1 cut(s) 405
Psp6I CCWGG 1 cut(s) 258
PspGI CCWGG 1 cut(s) 258
PspN4I GGNNCC 1 cut(s) 407
PspPI GGNCC 2 cut(s) 405, 466
PspPPI RGGWCCY 1 cut(s) 405
RseI CAYNNNNRTG 1 cut(s) 74
SaqAI TTAA 5 cut(s) 32, 110, 134, 141, 518
SatI GCNGC 1 cut(s) 285
Sau96I GGNCC 2 cut(s) 405, 466
SchI GAGTC 1 cut(s) 84
ScrFI CCNGG 1 cut(s) 260
SetI ASST 4 cut(s) 205, 249, 264, 326
SfaNI GCATC 2 cut(s) 4, 353
SfuI TTCGAA 1 cut(s) 194
SinI GGWCC 2 cut(s) 405, 466
SmiMI CAYNNNNRTG 1 cut(s) 74
SmlI CTYRAG 1 cut(s) 319
SmoI CTYRAG 1 cut(s) 319
SphI GCATGC 1 cut(s) 129
Sse9I AATT 6 cut(s) 131, 196, 308, 378, 399, 438
SspMI CTAG 4 cut(s) 248, 483, 492, 540
StyD4I CCNGG 1 cut(s) 258
StyI CCWWGG 2 cut(s) 98, 448
TaqI TCGA 1 cut(s) 194
TasI AATT 6 cut(s) 131, 196, 308, 378, 399, 438
TfiI GAWTC 2 cut(s) 444, 479
Tru1I TTAA 5 cut(s) 32, 110, 134, 141, 518
Tru9I TTAA 5 cut(s) 32, 110, 134, 141, 518
TseI GCWGC 1 cut(s) 284
TspDTI ATGAA 4 cut(s) 58, 92, 224, 377
VpaK11BI GGWCC 2 cut(s) 405, 466
XapI RAATTY 2 cut(s) 196, 308
XbaI TCTAGA 3 cut(s) 482, 491, 539
XceI RCATGY 2 cut(s) 129, 507
XspI CTAG 4 cut(s) 248, 483, 492, 540
Zsp2I ATGCAT 3 cut(s) 6, 19, 127
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.