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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AclWI | GGATC | 2 cut(s) | 18, 31 |
| AcsI | RAATTY | 2 cut(s) | 73, 249 |
| AgsI | TTSAA | 2 cut(s) | 72, 92 |
| AjuI | GAANNNNNNNTTGG | 2 cut(s) | 110, 142 |
| AlwI | GGATC | 2 cut(s) | 18, 31 |
| ApoI | RAATTY | 2 cut(s) | 73, 249 |
| ArsI | GACNNNNNNTTYG | 2 cut(s) | 197, 229 |
| AsuHPI | GGTGA | 1 cut(s) | 17 |
| BamHI | GGATCC | 1 cut(s) | 23 |
| BccI | CCATC | 1 cut(s) | 92 |
| BfaI | CTAG | 3 cut(s) | 14, 81, 246 |
| BmiI | GGNNCC | 1 cut(s) | 25 |
| BpuEI | CTTGAG | 1 cut(s) | 115 |
| BsaAI | YACGTR | 1 cut(s) | 107 |
| BsaBI | GATNNNNATC | 1 cut(s) | 165 |
| Bse8I | GATNNNNATC | 1 cut(s) | 165 |
| BseGI | GGATG | 1 cut(s) | 103 |
| BseJI | GATNNNNATC | 1 cut(s) | 165 |
| BsmI | GAATGC | 1 cut(s) | 212 |
| Bsp143I | GATC | 1 cut(s) | 23 |
| BspHI | TCATGA | 1 cut(s) | 266 |
| BspLI | GGNNCC | 1 cut(s) | 25 |
| BspPI | GGATC | 2 cut(s) | 18, 31 |
| BssMI | GATC | 1 cut(s) | 23 |
| BstBAI | YACGTR | 1 cut(s) | 107 |
| BstF5I | GGATG | 1 cut(s) | 103 |
| BstKTI | GATC | 1 cut(s) | 26 |
| BstMBI | GATC | 1 cut(s) | 23 |
| BstNSI | RCATGY | 1 cut(s) | 41 |
| BstSNI | TACGTA | 1 cut(s) | 107 |
| BstX2I | RGATCY | 1 cut(s) | 23 |
| BstYI | RGATCY | 1 cut(s) | 23 |
| BtsCI | GGATG | 1 cut(s) | 103 |
| CciI | TCATGA | 1 cut(s) | 266 |
| CviAII | CATG | 4 cut(s) | 38, 152, 232, 267 |
| CviJI | RGCY | 1 cut(s) | 13 |
| CviKI_1 | RGCY | 1 cut(s) | 13 |
| DpnI | GATC | 1 cut(s) | 25 |
| DpnII | GATC | 1 cut(s) | 23 |
| Eco105I | TACGTA | 1 cut(s) | 107 |
| FaeI | CATG | 4 cut(s) | 41, 155, 235, 270 |
| FatI | CATG | 4 cut(s) | 37, 151, 231, 266 |
| FokI | GGATG | 1 cut(s) | 110 |
| FspBI | CTAG | 3 cut(s) | 14, 81, 246 |
| Hin1II | CATG | 4 cut(s) | 41, 155, 235, 270 |
| HphI | GGTGA | 1 cut(s) | 17 |
| Hpy188III | TCNNGA | 1 cut(s) | 267 |
| HpyAV | CCTTC | 1 cut(s) | 37 |
| HpyCH4IV | ACGT | 1 cut(s) | 106 |
| HpyCH4V | TGCA | 2 cut(s) | 41, 231 |
| HpySE526I | ACGT | 1 cut(s) | 106 |
| Hsp92II | CATG | 4 cut(s) | 41, 155, 235, 270 |
| Kzo9I | GATC | 1 cut(s) | 23 |
| MaeI | CTAG | 3 cut(s) | 14, 81, 246 |
| MaeII | ACGT | 1 cut(s) | 106 |
| MaeIII | GTNAC | 1 cut(s) | 217 |
| MalI | GATC | 1 cut(s) | 25 |
| MboI | GATC | 1 cut(s) | 23 |
| MflI | RGATCY | 1 cut(s) | 23 |
| MluCI | AATT | 5 cut(s) | 56, 73, 92, 119, 249 |
| MnlI | CCTC | 1 cut(s) | 14 |
| MseI | TTAA | 2 cut(s) | 179, 257 |
| MslI | CAYNNNNRTG | 1 cut(s) | 265 |
| Mva1269I | GAATGC | 1 cut(s) | 212 |
| NdeII | GATC | 1 cut(s) | 23 |
| NlaIII | CATG | 4 cut(s) | 41, 155, 235, 270 |
| NlaIV | GGNNCC | 1 cut(s) | 25 |
| NspI | RCATGY | 1 cut(s) | 41 |
| PagI | TCATGA | 1 cut(s) | 266 |
| PctI | GAATGC | 1 cut(s) | 212 |
| Ppu21I | YACGTR | 1 cut(s) | 107 |
| PspN4I | GGNNCC | 1 cut(s) | 25 |
| PsuI | RGATCY | 1 cut(s) | 23 |
| RseI | CAYNNNNRTG | 1 cut(s) | 265 |
| SaqAI | TTAA | 2 cut(s) | 179, 257 |
| Sau3AI | GATC | 1 cut(s) | 23 |
| SetI | ASST | 1 cut(s) | 109 |
| SgeI | CNNG | 9 cut(s) | 26, 50, 93, 144, 164, 220, 244, 253, 258 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 265 |
| SmlI | CTYRAG | 1 cut(s) | 130 |
| SmoI | CTYRAG | 1 cut(s) | 130 |
| SnaBI | TACGTA | 1 cut(s) | 107 |
| Sse9I | AATT | 5 cut(s) | 56, 73, 92, 119, 249 |
| SspMI | CTAG | 3 cut(s) | 14, 81, 246 |
| TaiI | ACGT | 1 cut(s) | 109 |
| TaqI | TCGA | 1 cut(s) | 48 |
| TasI | AATT | 5 cut(s) | 56, 73, 92, 119, 249 |
| Tru1I | TTAA | 2 cut(s) | 179, 257 |
| Tru9I | TTAA | 2 cut(s) | 179, 257 |
| TspDTI | ATGAA | 4 cut(s) | 76, 159, 203, 239 |
| XapI | RAATTY | 2 cut(s) | 73, 249 |
| XceI | RCATGY | 1 cut(s) | 41 |
| XspI | CTAG | 3 cut(s) | 14, 81, 246 |