pycom520g01100

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
SuperScaffold_520
OG0000608
Physical Location & Seq
Forward (+)
817258 .. 818253
996 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom520g01100.1

Sequence Viewer

Length: 621 bp
ATGGAATTAGCGCATTGCAATCAAATTATTCCTCAAAAATCATTAATTTCTATGAAAATCATAACTGTTGACGAGGCGTTCATCACTATGAAACGCATGAGACTCATACCAAGGATTTACTTAACGCGATTTCTAGCATCAAATTCATGCATGCAAATTAAGTGTCTACCCCCAACCCACATACATAAACCAATAAAACATATAATCATGGCTTCGAATTCACCTCTAACTAAAAAGAAATTAGTTCCACATGTTCACAAAACAAAGATAACTAAACTTAAACATAAAAACAAAGATAGAATACATCTAGAAACACTCCAGGTGGCTCCTCCTTCCTTCCTTGCTGCCAACAATGGGGATTTGTGTGAATTTAGGCTCTTGAGGTATGGTGGATGGTGTGGTGCACAATTATCTAAACAGAAACCAAAGGGGAATAGGATAGGTAATGCATGGCTAGGTTTAAGGAAGGTTTCTAGAAAGAATGTTTTAGAACAGATTTTTGGTCCTAAAGGGATAAGGACATATGATATAGGTGTAGAGGAAGGATTGCAACTCTTAAACCTACATGGAATGGTGTTTATGCGATTTAGGGCACAACCTGATTTGGATAGGATAAGATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

207

Amino Acids

23.66

Weight (kDa)

10.37

Isoelectric Point (pI)

35.26

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 166
AccII CGCG 1 cut(s) 127
AcsI RAATTY 3 cut(s) 142, 217, 368
AfiI CCNNNNNNNGG 1 cut(s) 354
AflIII ACRYGT 1 cut(s) 250
AjnI CCWGG 1 cut(s) 318
AjuI GAANNNNNNNTTGG 2 cut(s) 483, 515
Alw21I GWGCWC 1 cut(s) 406
Alw26I GTCTC 1 cut(s) 94
Alw44I GTGCAC 1 cut(s) 402
ApaLI GTGCAC 1 cut(s) 402
ApeKI GCWGC 1 cut(s) 344
ApoI RAATTY 3 cut(s) 142, 217, 368
ArsI GACNNNNNNTTYG 2 cut(s) 148, 180
AseI ATTAAT 1 cut(s) 44
AspLEI GCGC 1 cut(s) 13
AspS9I GGNCC 1 cut(s) 503
AsuHPI GGTGA 1 cut(s) 213
AsuII TTCGAA 1 cut(s) 215
AvaII GGWCC 1 cut(s) 503
BaeGI GKGCMC 2 cut(s) 406, 595
Bbv12I GWGCWC 1 cut(s) 406
BbvI GCAGC 1 cut(s) 331
BccI CCATC 1 cut(s) 387
BciT130I CCWGG 1 cut(s) 320
BcoDI GTCTC 1 cut(s) 94
BfaI CTAG 4 cut(s) 134, 308, 455, 474
BisI GCNGC 1 cut(s) 345
BlsI GCNGC 1 cut(s) 346
Bme1390I CCNGG 1 cut(s) 320
Bme18I GGWCC 1 cut(s) 503
BmgT120I GGNCC 1 cut(s) 503
BmiI GGNNCC 1 cut(s) 327
BmrFI CCNGG 1 cut(s) 320
BmsI GCATC 1 cut(s) 146
BpmI CTGGAG 1 cut(s) 302
Bpu14I TTCGAA 1 cut(s) 215
BpuEI CTTGAG 1 cut(s) 400
BsaJI CCNNGG 1 cut(s) 110
Bsc4I CCNNNNNNNGG 1 cut(s) 354
Bse3DI GCAATG 1 cut(s) 13
BseBI CCWGG 1 cut(s) 320
BseDI CCNNGG 1 cut(s) 110
BseGI GGATG 1 cut(s) 398
BseLI CCNNNNNNNGG 1 cut(s) 354
BseMI GCAATG 1 cut(s) 13
BseRI GAGGAG 1 cut(s) 318
BseSI GKGCMC 2 cut(s) 406, 595
BseXI GCAGC 1 cut(s) 331
Bsh1236I CGCG 1 cut(s) 127
BsiHKAI GWGCWC 1 cut(s) 406
BslI CCNNNNNNNGG 1 cut(s) 354
BsmAI GTCTC 1 cut(s) 94
Bsp119I TTCGAA 1 cut(s) 215
Bsp1286I GDGCHC 2 cut(s) 406, 595
BspFNI CGCG 1 cut(s) 127
BspLI GGNNCC 1 cut(s) 327
BspT104I TTCGAA 1 cut(s) 215
BsrDI GCAATG 1 cut(s) 13
BssECI CCNNGG 1 cut(s) 110
BssT1I CCWWGG 1 cut(s) 110
Bst2UI CCWGG 1 cut(s) 320
Bst4CI ACNGT 1 cut(s) 67
BstBI TTCGAA 1 cut(s) 215
BstC8I GCNNGC 1 cut(s) 152
BstF5I GGATG 1 cut(s) 398
BstFNI CGCG 1 cut(s) 127
BstHHI GCGC 1 cut(s) 13
BstMAI GTCTC 1 cut(s) 94
BstNI CCWGG 1 cut(s) 320
BstNSI RCATGY 2 cut(s) 154, 254
BstSCI CCNGG 1 cut(s) 318
BstSLI GKGCMC 2 cut(s) 406, 595
BstUI CGCG 1 cut(s) 127
BstV1I GCAGC 1 cut(s) 331
BtsCI GGATG 1 cut(s) 398
Cac8I GCNNGC 1 cut(s) 152
CfoI GCGC 1 cut(s) 13
Cfr13I GGNCC 1 cut(s) 503
CviAII CATG 7 cut(s) 97, 147, 151, 208, 251, 450, 566
CviJI RGCY 4 cut(s) 212, 326, 376, 454
CviKI_1 RGCY 4 cut(s) 212, 326, 376, 454
Eco130I CCWWGG 1 cut(s) 110
Eco47I GGWCC 1 cut(s) 503
EcoRI GAATTC 1 cut(s) 217
EcoRII CCWGG 1 cut(s) 318
EcoT14I CCWWGG 1 cut(s) 110
EcoT22I ATGCAT 2 cut(s) 152, 451
ErhI CCWWGG 1 cut(s) 110
FaeI CATG 7 cut(s) 100, 150, 154, 211, 254, 453, 569
FatI CATG 7 cut(s) 96, 146, 150, 207, 250, 449, 565
FauNDI CATATG 1 cut(s) 523
FblI GTMKAC 1 cut(s) 166
Fnu4HI GCNGC 1 cut(s) 345
FokI GGATG 1 cut(s) 405
Fsp4HI GCNGC 1 cut(s) 345
FspBI CTAG 4 cut(s) 134, 308, 455, 474
GlaI GCGC 1 cut(s) 12
GluI GCNGC 1 cut(s) 345
GsuI CTGGAG 1 cut(s) 302
HhaI GCGC 1 cut(s) 13
Hin1II CATG 7 cut(s) 100, 150, 154, 211, 254, 453, 569
Hin6I GCGC 1 cut(s) 11
HinP1I GCGC 1 cut(s) 11
HincII GTYRAC 1 cut(s) 70
HindII GTYRAC 1 cut(s) 70
HinfI GANTC 1 cut(s) 102
HphI GGTGA 1 cut(s) 213
Hpy166II GTNNAC 4 cut(s) 70, 167, 256, 404
Hpy188III TCNNGA 3 cut(s) 308, 379, 474
Hpy8I GTNNAC 4 cut(s) 70, 167, 256, 404
HpyAV CCTTC 4 cut(s) 342, 346, 460, 536
HpyCH4III ACNGT 1 cut(s) 67
HpyCH4V TGCA 6 cut(s) 18, 150, 154, 404, 449, 550
Hsp92II CATG 7 cut(s) 100, 150, 154, 211, 254, 453, 569
HspAI GCGC 1 cut(s) 11
LmnI GCTCC 1 cut(s) 331
LpnPI CCDG 3 cut(s) 305, 332, 612
Lsp1109I GCAGC 1 cut(s) 331
LweI GCATC 1 cut(s) 146
MaeI CTAG 4 cut(s) 134, 308, 455, 474
MhlI GDGCHC 2 cut(s) 406, 595
MluCI AATT 9 cut(s) 5, 24, 45, 142, 156, 217, 239, 368, 407
MlyI GAGTC 1 cut(s) 96
MnlI CCTC 6 cut(s) 42, 67, 234, 339, 375, 532
Mph1103I ATGCAT 2 cut(s) 152, 451
MseI TTAA 6 cut(s) 44, 122, 159, 279, 461, 557
MslI CAYNNNNRTG 1 cut(s) 86
MspR9I CCNGG 1 cut(s) 320
MvaI CCWGG 1 cut(s) 320
MvnI CGCG 1 cut(s) 127
NdeI CATATG 1 cut(s) 523
NlaIII CATG 7 cut(s) 100, 150, 154, 211, 254, 453, 569
NlaIV GGNNCC 1 cut(s) 327
NsiI ATGCAT 2 cut(s) 152, 451
NspI RCATGY 2 cut(s) 154, 254
NspV TTCGAA 1 cut(s) 215
PaeI GCATGC 1 cut(s) 154
PciI ACATGT 1 cut(s) 250
PkrI GCNGC 1 cut(s) 346
PleI GAGTC 1 cut(s) 96
PpsI GAGTC 1 cut(s) 96
PscI ACATGT 1 cut(s) 250
PshBI ATTAAT 1 cut(s) 44
Psp6I CCWGG 1 cut(s) 318
PspGI CCWGG 1 cut(s) 318
PspN4I GGNNCC 1 cut(s) 327
PspPI GGNCC 1 cut(s) 503
RseI CAYNNNNRTG 1 cut(s) 86
SaqAI TTAA 6 cut(s) 44, 122, 159, 279, 461, 557
SatI GCNGC 1 cut(s) 345
Sau96I GGNCC 1 cut(s) 503
SchI GAGTC 1 cut(s) 96
ScrFI CCNGG 1 cut(s) 320
SduI GDGCHC 2 cut(s) 406, 595
SetI ASST 9 cut(s) 226, 324, 386, 445, 460, 471, 535, 564, 601
SfaNI GCATC 1 cut(s) 146
SfuI TTCGAA 1 cut(s) 215
SinI GGWCC 1 cut(s) 503
SmiMI CAYNNNNRTG 1 cut(s) 86
SmlI CTYRAG 1 cut(s) 379
SmoI CTYRAG 1 cut(s) 379
SphI GCATGC 1 cut(s) 154
Sse9I AATT 9 cut(s) 5, 24, 45, 142, 156, 217, 239, 368, 407
SspMI CTAG 4 cut(s) 134, 308, 455, 474
StyD4I CCNGG 1 cut(s) 318
StyI CCWWGG 1 cut(s) 110
TaaI ACNGT 1 cut(s) 67
TaqI TCGA 1 cut(s) 215
TasI AATT 9 cut(s) 5, 24, 45, 142, 156, 217, 239, 368, 407
Tru1I TTAA 6 cut(s) 44, 122, 159, 279, 461, 557
Tru9I TTAA 6 cut(s) 44, 122, 159, 279, 461, 557
TseI GCWGC 1 cut(s) 344
TspDTI ATGAA 4 cut(s) 68, 70, 104, 135
VneI GTGCAC 1 cut(s) 402
VpaK11BI GGWCC 1 cut(s) 503
VspI ATTAAT 1 cut(s) 44
XapI RAATTY 3 cut(s) 142, 217, 368
XbaI TCTAGA 2 cut(s) 307, 473
XceI RCATGY 2 cut(s) 154, 254
XmiI GTMKAC 1 cut(s) 166
XspI CTAG 4 cut(s) 134, 308, 455, 474
Zsp2I ATGCAT 2 cut(s) 152, 451
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.