pycom05g22500

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr5
OG0000608
Physical Location & Seq
Forward (+)
24801690 .. 24802833
1144 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom05g22500.3

Sequence Viewer

Length: 810 bp
ATGAATGTATTTAAAAAAAAAAATTGGCCCAAAAAACCAAAAATTAAAAAAAATAAATTGAATACAACGGCTATGACAAGAATACATTTAATTTTTAATATAAATATATTCCTGTCGGTTATAACCGACATGAAAGCTATATTTTCTGGCCAGCCCCGGGCTATCTGGTTGGCTACTTGCAGCCAACCCTCCAGCCCTCTAGCCCATTCCGATTCCGTGGGCCCTCTCAGATTCCACAGCCATCTGACTAGCCCTCGGTTGGAGATGGTTTTTGGGCTATTTTCGACATTCGTATGTCACTGTACAATCATGCTCCAATCCTTGTGTTCGCGAGAACCGGATGTAGTAAAGATGCAAGTAGGGATCGTTCTAGACCGGGGATTAGGAGGGCTTTCTAAAACCTCTAAACTGACTCAAAAACATAAAAACAAAGTTAAAAACGTTCACAAAGACTCAAAGGACTCAAAACAAGGTCAAAAGACTCAAAACAGGTTCCAAAAGGCTCAAAACTGCCTAACAACACTAACTAGGCAGTTTCTGACCTTAAACCCAATTTTGGACGAATTTAGAATTATGAACAAGAAAGTAAAGGGGGGTTTTGTTTTGACGAAAATTGAAATAACAAAAAACAAGATGATGGATGGATTAGTTAGAGGTCCATTCTCCACACATGACACATTTATATATGAATCGATTCTCCAATTGCTTTTCATTAACTATGATGCTCAACACCCCAAGTTAATCGTGATGCGACAATTCAAATCATTCTCCAATGGTTCTCAACATGAATGCATAGAAGAGATACAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

270

Amino Acids

30.78

Weight (kDa)

10.02

Isoelectric Point (pI)

43.54

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 122
AccII CGCG 1 cut(s) 331
AclI AACGTT 1 cut(s) 441
AclWI GGATC 1 cut(s) 371
AcoI YGGCCR 1 cut(s) 148
AcsI RAATTY 1 cut(s) 563
AfaI GTAC 1 cut(s) 304
AfiI CCNNNNNNNGG 3 cut(s) 157, 259, 556
AgsI TTSAA 3 cut(s) 61, 617, 760
AluBI AGCT 1 cut(s) 137
AluI AGCT 1 cut(s) 137
AlwI GGATC 1 cut(s) 371
AlwNI CAGNNNCTG 1 cut(s) 538
Ama87I CYCGRG 1 cut(s) 156
AoxI GGCC 3 cut(s) 26, 148, 220
ApaI GGGCCC 1 cut(s) 224
ApeKI GCWGC 1 cut(s) 180
ApoI RAATTY 1 cut(s) 563
Asp700I GAANNNNTTC 1 cut(s) 693
AspS9I GGNCC 4 cut(s) 27, 220, 221, 656
AsuC2I CCSGG 3 cut(s) 157, 158, 377
AvaI CYCGRG 1 cut(s) 156
AvaII GGWCC 1 cut(s) 656
BaeGI GKGCMC 1 cut(s) 224
BalI TGGCCA 1 cut(s) 150
BanII GRGCYC 1 cut(s) 224
BbvI GCAGC 1 cut(s) 192
BccI CCATC 4 cut(s) 249, 259, 631, 635
BceAI ACGGC 1 cut(s) 84
BcnI CCSGG 3 cut(s) 157, 158, 377
BfaI CTAG 4 cut(s) 200, 249, 371, 528
BisI GCNGC 1 cut(s) 181
BlsI GCNGC 1 cut(s) 182
Bme1390I CCNGG 3 cut(s) 157, 158, 377
Bme18I GGWCC 1 cut(s) 656
BmeT110I CYCGRG 1 cut(s) 156
BmgT120I GGNCC 4 cut(s) 27, 220, 221, 656
BmiI GGNNCC 2 cut(s) 222, 494
BmrFI CCNGG 3 cut(s) 157, 158, 377
BmsI GCATC 3 cut(s) 342, 712, 738
BpmI CTGGAG 1 cut(s) 175
BpuMI CCSGG 3 cut(s) 157, 158, 377
Bsa29I ATCGAT 1 cut(s) 692
BsaJI CCNNGG 5 cut(s) 155, 156, 216, 254, 376
BsaWI WCCGGW 1 cut(s) 337
Bsc4I CCNNNNNNNGG 3 cut(s) 157, 259, 556
BseCI ATCGAT 1 cut(s) 692
BseDI CCNNGG 5 cut(s) 155, 156, 216, 254, 376
BseGI GGATG 2 cut(s) 346, 646
BseLI CCNNNNNNNGG 3 cut(s) 157, 259, 556
BseMII CTCAG 1 cut(s) 241
BseSI GKGCMC 1 cut(s) 224
BseXI GCAGC 1 cut(s) 192
Bsh1236I CGCG 1 cut(s) 331
BshFI GGCC 3 cut(s) 28, 150, 222
BshVI ATCGAT 1 cut(s) 692
BsiHKCI CYCGRG 1 cut(s) 156
BsiSI CCGG 3 cut(s) 157, 338, 376
BslI CCNNNNNNNGG 3 cut(s) 157, 259, 556
BsmI GAATGC 1 cut(s) 794
BsnI GGCC 3 cut(s) 28, 150, 222
BsoBI CYCGRG 1 cut(s) 156
Bsp120I GGGCCC 1 cut(s) 220
Bsp1286I GDGCHC 1 cut(s) 224
Bsp1407I TGTACA 1 cut(s) 302
Bsp143I GATC 1 cut(s) 363
Bsp68I TCGCGA 1 cut(s) 331
BspANI GGCC 3 cut(s) 28, 150, 222
BspCNI CTCAG 1 cut(s) 240
BspDI ATCGAT 1 cut(s) 692
BspFNI CGCG 1 cut(s) 331
BspLI GGNNCC 2 cut(s) 222, 494
BspPI GGATC 1 cut(s) 371
BsrGI TGTACA 1 cut(s) 302
BssECI CCNNGG 5 cut(s) 155, 156, 216, 254, 376
BssMI GATC 1 cut(s) 363
Bst4CI ACNGT 1 cut(s) 302
Bst6I CTCTTC 1 cut(s) 792
BstAUI TGTACA 1 cut(s) 302
BstC8I GCNNGC 1 cut(s) 152
BstDEI CTNAG 1 cut(s) 227
BstDSI CCRYGG 1 cut(s) 216
BstF5I GGATG 2 cut(s) 346, 646
BstFNI CGCG 1 cut(s) 331
BstKTI GATC 1 cut(s) 366
BstMBI GATC 1 cut(s) 363
BstSCI CCNGG 3 cut(s) 155, 156, 375
BstSLI GKGCMC 1 cut(s) 224
BstUI CGCG 1 cut(s) 331
BstV1I GCAGC 1 cut(s) 192
Bsu15I ATCGAT 1 cut(s) 692
BsuRI GGCC 3 cut(s) 28, 150, 222
BsuTUI ATCGAT 1 cut(s) 692
BtgI CCRYGG 1 cut(s) 216
BtsCI GGATG 2 cut(s) 346, 646
BtsIMutI CAGTG 1 cut(s) 298
BtuMI TCGCGA 1 cut(s) 331
Cac8I GCNNGC 1 cut(s) 152
CaiI CAGNNNCTG 1 cut(s) 538
Cfr13I GGNCC 4 cut(s) 27, 220, 221, 656
Cfr9I CCCGGG 1 cut(s) 156
ClaI ATCGAT 1 cut(s) 692
Csp6I GTAC 1 cut(s) 303
CviAII CATG 4 cut(s) 130, 310, 671, 785
CviQI GTAC 1 cut(s) 303
DdeI CTNAG 1 cut(s) 227
DpnI GATC 1 cut(s) 365
DpnII GATC 1 cut(s) 363
DraI TTTAAA 1 cut(s) 13
EaeI YGGCCR 1 cut(s) 148
Eam1104I CTCTTC 1 cut(s) 792
EarI CTCTTC 1 cut(s) 792
Eco24I GRGCYC 1 cut(s) 224
Eco47I GGWCC 1 cut(s) 656
Eco88I CYCGRG 1 cut(s) 156
EcoO109I RGGNCCY 1 cut(s) 221
EcoT22I ATGCAT 1 cut(s) 794
EcoT38I GRGCYC 1 cut(s) 224
FaeI CATG 4 cut(s) 133, 313, 674, 788
FatI CATG 4 cut(s) 129, 309, 670, 784
Fnu4HI GCNGC 1 cut(s) 181
FokI GGATG 2 cut(s) 353, 653
FriOI GRGCYC 1 cut(s) 224
Fsp4HI GCNGC 1 cut(s) 181
FspBI CTAG 4 cut(s) 200, 249, 371, 528
GluI GCNGC 1 cut(s) 181
GsuI CTGGAG 1 cut(s) 175
HaeIII GGCC 3 cut(s) 28, 150, 222
HapII CCGG 3 cut(s) 157, 338, 376
Hin1II CATG 4 cut(s) 133, 313, 674, 788
HinfI GANTC 8 cut(s) 212, 231, 412, 452, 461, 481, 689, 694
HpaII CCGG 3 cut(s) 157, 338, 376
Hpy166II GTNNAC 1 cut(s) 445
Hpy188I TCNGA 4 cut(s) 211, 230, 246, 540
Hpy188III TCNNGA 3 cut(s) 330, 371, 745
Hpy8I GTNNAC 1 cut(s) 445
HpyCH4III ACNGT 1 cut(s) 302
HpyCH4IV ACGT 1 cut(s) 441
HpyCH4V TGCA 3 cut(s) 180, 355, 792
HpyF3I CTNAG 1 cut(s) 227
HpySE526I ACGT 1 cut(s) 441
Hsp92II CATG 4 cut(s) 133, 313, 674, 788
Kzo9I GATC 1 cut(s) 363
LmnI GCTCC 1 cut(s) 318
LpnPI CCDG 9 cut(s) 125, 132, 151, 164, 170, 205, 351, 389, 475
Lsp1109I GCAGC 1 cut(s) 192
LweI GCATC 3 cut(s) 342, 712, 738
MaeI CTAG 4 cut(s) 200, 249, 371, 528
MaeII ACGT 1 cut(s) 441
MaeIII GTNAC 1 cut(s) 296
MalI GATC 1 cut(s) 365
MboI GATC 1 cut(s) 363
MboII GAAGA 1 cut(s) 809
MfeI CAATTG 1 cut(s) 701
MhlI GDGCHC 1 cut(s) 224
MlsI TGGCCA 1 cut(s) 150
MluNI TGGCCA 1 cut(s) 150
MlyI GAGTC 4 cut(s) 406, 446, 455, 475
MmeI TCCRAC 1 cut(s) 240
MnlI CCTC 7 cut(s) 199, 207, 234, 264, 380, 412, 647
Mox20I TGGCCA 1 cut(s) 150
Mph1103I ATGCAT 1 cut(s) 794
MroXI GAANNNNTTC 1 cut(s) 693
MscI TGGCCA 1 cut(s) 150
MseI TTAA 8 cut(s) 12, 45, 89, 96, 435, 545, 714, 740
MslI CAYNNNNRTG 1 cut(s) 292
Msp20I TGGCCA 1 cut(s) 150
MspI CCGG 3 cut(s) 157, 338, 376
MspR9I CCNGG 3 cut(s) 157, 158, 377
MunI CAATTG 1 cut(s) 701
Mva1269I GAATGC 1 cut(s) 794
MvnI CGCG 1 cut(s) 331
NciI CCSGG 3 cut(s) 157, 158, 377
NdeII GATC 1 cut(s) 363
NlaIII CATG 4 cut(s) 133, 313, 674, 788
NlaIV GGNNCC 2 cut(s) 222, 494
NmuCI GTSAC 1 cut(s) 296
NruI TCGCGA 1 cut(s) 331
NsiI ATGCAT 1 cut(s) 794
PctI GAATGC 1 cut(s) 794
PdmI GAANNNNTTC 1 cut(s) 693
PfeI GAWTC 4 cut(s) 212, 231, 689, 694
PkrI GCNGC 1 cut(s) 182
PleI GAGTC 4 cut(s) 406, 446, 455, 475
PpsI GAGTC 4 cut(s) 406, 446, 455, 475
PsiI TTATAA 1 cut(s) 122
Psp1406I AACGTT 1 cut(s) 441
PspN4I GGNNCC 2 cut(s) 222, 494
PspOMI GGGCCC 1 cut(s) 220
PspPI GGNCC 4 cut(s) 27, 220, 221, 656
PsrI GAACNNNNNNTAC 2 cut(s) 351, 383
PstNI CAGNNNCTG 1 cut(s) 538
RruI TCGCGA 1 cut(s) 331
RsaI GTAC 1 cut(s) 304
RsaNI GTAC 1 cut(s) 303
RseI CAYNNNNRTG 1 cut(s) 292
SaqAI TTAA 8 cut(s) 12, 45, 89, 96, 435, 545, 714, 740
SatI GCNGC 1 cut(s) 181
Sau3AI GATC 1 cut(s) 363
Sau96I GGNCC 4 cut(s) 27, 220, 221, 656
SchI GAGTC 4 cut(s) 406, 446, 455, 475
ScrFI CCNGG 3 cut(s) 157, 158, 377
SduI GDGCHC 1 cut(s) 224
SetI ASST 7 cut(s) 139, 404, 444, 475, 494, 545, 658
SfaNI GCATC 3 cut(s) 342, 712, 738
SinI GGWCC 1 cut(s) 656
SmaI CCCGGG 1 cut(s) 158
SmiMI CAYNNNNRTG 1 cut(s) 292
SspMI CTAG 4 cut(s) 200, 249, 371, 528
StyD4I CCNGG 3 cut(s) 155, 156, 375
TaaI ACNGT 1 cut(s) 302
TaiI ACGT 1 cut(s) 444
TaqI TCGA 2 cut(s) 284, 692
TatI WGTACW 1 cut(s) 302
TfiI GAWTC 4 cut(s) 212, 231, 689, 694
Tru1I TTAA 8 cut(s) 12, 45, 89, 96, 435, 545, 714, 740
Tru9I TTAA 8 cut(s) 12, 45, 89, 96, 435, 545, 714, 740
TscAI CASTG 1 cut(s) 305
TseFI GTSAC 1 cut(s) 296
TseI GCWGC 1 cut(s) 180
Tsp45I GTSAC 1 cut(s) 296
TspDTI ATGAA 6 cut(s) 17, 146, 590, 700, 702, 801
TspGWI ACGGA 1 cut(s) 205
TspMI CCCGGG 1 cut(s) 156
TspRI CASTG 1 cut(s) 305
VpaK11BI GGWCC 1 cut(s) 656
XapI RAATTY 1 cut(s) 563
XbaI TCTAGA 1 cut(s) 370
XmaI CCCGGG 1 cut(s) 156
XmnI GAANNNNTTC 1 cut(s) 693
XspI CTAG 4 cut(s) 200, 249, 371, 528
Zsp2I ATGCAT 1 cut(s) 794
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.