pycom12661g00070

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
tig00012661
OG0000608
Physical Location & Seq
Forward (+)
50304 .. 51450
1147 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom12661g00070.7

Sequence Viewer

Length: 840 bp
ATGTGGCTCAAATGTGGAAGGTGTGTGGCTACTTCAAGTAGGAATCCTTGTGACATCAACTCTTCAATTTCGTCCATTCCTTTTGCTCCAAGTATAAGTTATCCCTTCCAAGTCCAATTTTGCTCCATAATGCTCCAAAATGCATCTTTTTGCTTCCTTAGCCACATGAACCTAAAAACACACGAAAATGTAAGTCGCCTAAATATGCTCCTATCATTCTTCAATAGTTCCCTACATGAACAACAGAATAGAGATACAATCAAAGATCGATTAAGCTTTGTGAAAATCATAAGCATTGACAAGGCATTCGTAACTATGAACAACATGATACTCCTGCCAGGAATTTACTTAACACGATCTCTAGCATCAAATTCATGCGTGCAAACTAACAAACAGTTAAGTAAATTGCATTCACGATTTATGAAATCACAACTGGAGGTAATCAATTCATATTGCAAATATAATCATGGTTTCAAAGTTTCCTCTAGCCAAAACGAAATTATGAAAACGAAAGATGGATTACACCTAAGAACTCTCCAACAATCCAATCTTGAATGGCAAGCACGTCCCAAGGTCCTCATTCTTCTCCTTTGCTGCAGCACAAAGTGTATGGTAGTGAATGGATCCCTGAAAAGCTGCGGCAAGGCTCTTATTTATATGGGAAGGAAAGGGCACGGCTGGGAATTAAATTTGGAAAGGTTTAAGACTCCAAATCCTCCAAGAAAAGGCCTAGAAAGAAAGGGAAAGGAAGGGAAAGGGTGTGGCATCCCTCTTAGATTAGGATTAATGCTTCTGGAGCTGATATTTAAGTATCTTAAATTAATTAGGAACCCCCCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

280

Amino Acids

31.76

Weight (kDa)

9.85

Isoelectric Point (pI)

30.35

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 639
AclWI GGATC 2 cut(s) 618, 631
AcsI RAATTY 3 cut(s) 342, 370, 688
AdeI CACNNNGTG 1 cut(s) 606
AfiI CCNNNNNNNGG 1 cut(s) 725
AgsI TTSAA 5 cut(s) 36, 66, 223, 475, 554
AjiI CACGTC 1 cut(s) 566
AjnI CCWGG 1 cut(s) 337
AluBI AGCT 3 cut(s) 276, 636, 799
AluI AGCT 3 cut(s) 276, 636, 799
AlwI GGATC 2 cut(s) 618, 631
AoxI GGCC 1 cut(s) 727
ApeKI GCWGC 3 cut(s) 594, 597, 636
ApoI RAATTY 3 cut(s) 342, 370, 688
ArsI GACNNNNNNTTYG 2 cut(s) 290, 322
AseI ATTAAT 2 cut(s) 785, 821
AspS9I GGNCC 1 cut(s) 574
AvaII GGWCC 1 cut(s) 574
BaeGI GKGCMC 1 cut(s) 675
BamHI GGATCC 1 cut(s) 623
BbvI GCAGC 3 cut(s) 581, 609, 623
BccI CCATC 1 cut(s) 509
BceAI ACGGC 1 cut(s) 691
BciT130I CCWGG 1 cut(s) 339
BfaI CTAG 3 cut(s) 362, 486, 731
BfmI CTRYAG 1 cut(s) 595
BisI GCNGC 4 cut(s) 595, 598, 637, 640
BlsI GCNGC 4 cut(s) 596, 599, 638, 641
Bme1390I CCNGG 1 cut(s) 339
Bme18I GGWCC 1 cut(s) 574
BmgBI CACGTC 1 cut(s) 566
BmgT120I GGNCC 1 cut(s) 574
BmiI GGNNCC 2 cut(s) 625, 830
BmrFI CCNGG 1 cut(s) 339
BmsI GCATC 3 cut(s) 152, 374, 774
BpmI CTGGAG 2 cut(s) 455, 815
Bpu10I CCTNAGC 1 cut(s) 158
Bsa29I ATCGAT 1 cut(s) 268
BsaJI CCNNGG 1 cut(s) 570
Bsc4I CCNNNNNNNGG 1 cut(s) 725
Bse1I ACTGG 1 cut(s) 438
BseBI CCWGG 1 cut(s) 339
BseCI ATCGAT 1 cut(s) 268
BseDI CCNNGG 1 cut(s) 570
BseGI GGATG 1 cut(s) 765
BseLI CCNNNNNNNGG 1 cut(s) 725
BseNI ACTGG 1 cut(s) 438
BseSI GKGCMC 1 cut(s) 675
BseXI GCAGC 3 cut(s) 581, 609, 623
BseYI CCCAGC 1 cut(s) 678
BshFI GGCC 1 cut(s) 729
BshVI ATCGAT 1 cut(s) 268
BslFI GGGAC 1 cut(s) 552
BslI CCNNNNNNNGG 1 cut(s) 725
BsmFI GGGAC 1 cut(s) 552
BsmI GAATGC 2 cut(s) 305, 409
BsnI GGCC 1 cut(s) 729
Bsp1286I GDGCHC 1 cut(s) 675
Bsp143I GATC 3 cut(s) 265, 356, 623
BspACI CCGC 1 cut(s) 639
BspANI GGCC 1 cut(s) 729
BspDI ATCGAT 1 cut(s) 268
BspLI GGNNCC 2 cut(s) 625, 830
BspMAI CTGCAG 1 cut(s) 599
BspPI GGATC 2 cut(s) 618, 631
BsrI ACTGG 1 cut(s) 438
BssECI CCNNGG 1 cut(s) 570
BssMI GATC 3 cut(s) 265, 356, 623
BssT1I CCWWGG 1 cut(s) 570
Bst2UI CCWGG 1 cut(s) 339
Bst4CI ACNGT 1 cut(s) 396
Bst6I CTCTTC 1 cut(s) 67
BstC8I GCNNGC 2 cut(s) 380, 561
BstDEI CTNAG 3 cut(s) 158, 527, 773
BstF5I GGATG 1 cut(s) 765
BstKTI GATC 3 cut(s) 268, 359, 626
BstMBI GATC 3 cut(s) 265, 356, 623
BstMWI GCNNNNNNNGC 2 cut(s) 159, 796
BstNI CCWGG 1 cut(s) 339
BstSCI CCNGG 1 cut(s) 337
BstSFI CTRYAG 1 cut(s) 595
BstSLI GKGCMC 1 cut(s) 675
BstV1I GCAGC 3 cut(s) 581, 609, 623
BstX2I RGATCY 1 cut(s) 623
BstYI RGATCY 1 cut(s) 623
Bsu15I ATCGAT 1 cut(s) 268
BsuRI GGCC 1 cut(s) 729
BsuTUI ATCGAT 1 cut(s) 268
BtrI CACGTC 1 cut(s) 566
BtsCI GGATG 1 cut(s) 765
Cac8I GCNNGC 2 cut(s) 380, 561
Cfr13I GGNCC 1 cut(s) 574
ClaI ATCGAT 1 cut(s) 268
CviAII CATG 5 cut(s) 166, 236, 325, 375, 467
DdeI CTNAG 3 cut(s) 158, 527, 773
DpnI GATC 3 cut(s) 267, 358, 625
DpnII GATC 3 cut(s) 265, 356, 623
DraIII CACNNNGTG 1 cut(s) 606
Eam1104I CTCTTC 1 cut(s) 67
EarI CTCTTC 1 cut(s) 67
Eco130I CCWWGG 1 cut(s) 570
Eco147I AGGCCT 1 cut(s) 729
Eco47I GGWCC 1 cut(s) 574
EcoO109I RGGNCCY 1 cut(s) 574
EcoRII CCWGG 1 cut(s) 337
EcoT14I CCWWGG 1 cut(s) 570
EcoT22I ATGCAT 1 cut(s) 145
ErhI CCWWGG 1 cut(s) 570
FaeI CATG 5 cut(s) 169, 239, 328, 378, 470
FaqI GGGAC 1 cut(s) 552
FatI CATG 5 cut(s) 165, 235, 324, 374, 466
Fnu4HI GCNGC 4 cut(s) 595, 598, 637, 640
FokI GGATG 1 cut(s) 752
Fsp4HI GCNGC 4 cut(s) 595, 598, 637, 640
FspBI CTAG 3 cut(s) 362, 486, 731
GluI GCNGC 4 cut(s) 595, 598, 637, 640
GsaI CCCAGC 1 cut(s) 682
GsuI CTGGAG 2 cut(s) 455, 815
HaeIII GGCC 1 cut(s) 729
Hin1II CATG 5 cut(s) 169, 239, 328, 378, 470
HindIII AAGCTT 1 cut(s) 274
HinfI GANTC 2 cut(s) 43, 706
Hpy188III TCNNGA 3 cut(s) 414, 551, 794
HpyAV CCTTC 4 cut(s) 12, 115, 657, 743
HpyCH4III ACNGT 1 cut(s) 396
HpyCH4IV ACGT 1 cut(s) 565
HpyCH4V TGCA 5 cut(s) 143, 382, 409, 456, 597
HpyF10VI GCNNNNNNNGC 2 cut(s) 159, 796
HpyF3I CTNAG 3 cut(s) 158, 527, 773
HpySE526I ACGT 1 cut(s) 565
Hsp92II CATG 5 cut(s) 169, 239, 328, 378, 470
Kzo9I GATC 3 cut(s) 265, 356, 623
LmnI GCTCC 5 cut(s) 91, 128, 138, 213, 796
LpnPI CCDG 7 cut(s) 324, 347, 351, 419, 641, 664, 779
Lsp1109I GCAGC 3 cut(s) 581, 609, 623
LweI GCATC 3 cut(s) 152, 374, 774
MaeI CTAG 3 cut(s) 362, 486, 731
MaeII ACGT 1 cut(s) 565
MaeIII GTNAC 2 cut(s) 50, 310
MalI GATC 3 cut(s) 267, 358, 625
MboI GATC 3 cut(s) 265, 356, 623
MboII GAAGA 3 cut(s) 54, 211, 575
MflI RGATCY 1 cut(s) 623
MhlI GDGCHC 1 cut(s) 675
MlyI GAGTC 1 cut(s) 700
MmeI TCCRAC 1 cut(s) 562
MnlI CCTC 5 cut(s) 430, 493, 587, 726, 780
Mph1103I ATGCAT 1 cut(s) 145
MslI CAYNNNNRTG 1 cut(s) 186
MspR9I CCNGG 1 cut(s) 339
Mva1269I GAATGC 2 cut(s) 305, 409
MvaI CCWGG 1 cut(s) 339
MwoI GCNNNNNNNGC 2 cut(s) 159, 796
NdeII GATC 3 cut(s) 265, 356, 623
NlaIII CATG 5 cut(s) 169, 239, 328, 378, 470
NlaIV GGNNCC 2 cut(s) 625, 830
NmuCI GTSAC 1 cut(s) 50
NsiI ATGCAT 1 cut(s) 145
PceI AGGCCT 1 cut(s) 729
PctI GAATGC 2 cut(s) 305, 409
PfeI GAWTC 1 cut(s) 43
PkrI GCNGC 4 cut(s) 596, 599, 638, 641
PleI GAGTC 1 cut(s) 700
PpsI GAGTC 1 cut(s) 700
PpuMI RGGWCCY 1 cut(s) 574
PshBI ATTAAT 2 cut(s) 785, 821
Psp5II RGGWCCY 1 cut(s) 574
Psp6I CCWGG 1 cut(s) 337
PspFI CCCAGC 1 cut(s) 678
PspGI CCWGG 1 cut(s) 337
PspN4I GGNNCC 2 cut(s) 625, 830
PspPI GGNCC 1 cut(s) 574
PspPPI RGGWCCY 1 cut(s) 574
PstI CTGCAG 1 cut(s) 599
PsuI RGATCY 1 cut(s) 623
RseI CAYNNNNRTG 1 cut(s) 186
SatI GCNGC 4 cut(s) 595, 598, 637, 640
Sau3AI GATC 3 cut(s) 265, 356, 623
Sau96I GGNCC 1 cut(s) 574
SchI GAGTC 1 cut(s) 700
ScrFI CCNGG 1 cut(s) 339
SduI GDGCHC 1 cut(s) 675
SfaNI GCATC 3 cut(s) 152, 374, 774
SfcI CTRYAG 1 cut(s) 595
SinI GGWCC 1 cut(s) 574
SmiMI CAYNNNNRTG 1 cut(s) 186
SseBI AGGCCT 1 cut(s) 729
SsiI CCGC 1 cut(s) 639
SspMI CTAG 3 cut(s) 362, 486, 731
StuI AGGCCT 1 cut(s) 729
StyD4I CCNGG 1 cut(s) 337
StyI CCWWGG 1 cut(s) 570
TaaI ACNGT 1 cut(s) 396
TaiI ACGT 1 cut(s) 568
TaqI TCGA 1 cut(s) 268
TauI GCSGC 1 cut(s) 642
TfiI GAWTC 1 cut(s) 43
TseFI GTSAC 1 cut(s) 50
TseI GCWGC 3 cut(s) 594, 597, 636
Tsp45I GTSAC 1 cut(s) 50
TspDTI ATGAA 7 cut(s) 182, 252, 332, 363, 437, 438, 518
VpaK11BI GGWCC 1 cut(s) 574
VspI ATTAAT 2 cut(s) 785, 821
XapI RAATTY 3 cut(s) 342, 370, 688
XspI CTAG 3 cut(s) 362, 486, 731
Zsp2I ATGCAT 1 cut(s) 145
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.