pycom436g00360

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
SuperScaffold_436
OG0000608
Physical Location & Seq
Forward (+)
396916 .. 398845
1930 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom436g00360.12

Sequence Viewer

Length: 1179 bp
ATGAAGAGGTGGTGGTTTCTGGGCTGTTTTGATGATGTAGAATGGATTTTGGGGTGGTTAGTGATGCGAAAATATAAGGATGCAAGTAGGGATCGTTCTGGACCGGGGATTAGGAGGGCTTTTAAAAACGTTCACAAAGACTCAAAGGACTCAAAACAGGTTCAAAAGACTCAAAACTGGGTTGACTCAAAACACTTTAAAACACAAACTAAACAGATTCTAAACAATATAGAACAAGAAAATTGTAAAACGAATTTTGGAAACAAGATGATGGATGGATTAGTTAGAGGTCCATTCTCCACACATGACACATTTATATATGAATCGATTTTCCAATTGCTTTTCATTATCTATGATGCTCAACACCCCAAGTTATTATTGAATTGGATGGGAATGCGCATACAACAACTCAAAACATTCTCTAAAAGTCCCTTACGTGAAAAGCACAATAAAGGCAATTGTTACTATGAAAAGCATGAAACTCTTGCCAAGAATTCAATTAACGCGGTTGTGACTAGCAACCTTCACTACTCATCATCGAGTTTATGCATGAAGACTAAGTATGCATCCCTAATCAACATACAAAAACAAGTTTTTAATAAACAGATAATTGAATTGCAATCACAACTTAACAAATCACAATTGGGGTTTAAACGAAGTACACCTAAAACACTCCAATCTGCAAACATGAAACGTTTTAGGCCTCCTTCCTCTCCTAGTTGCTGCGGCACAAGGGGTTATGGTGAAGGGATGGATGTAGGGGACGGCAAGGGTTGTTTTTGGGCAGAAAGTATGAAGAATGGTGAAGTATGGATGAATGAATGTTTTGTGGCTGCAACAAGGAGGTTTATTAATAGGATTTCAGGAATTAAAACCCTAGGTTATTTAGGTAATCAGAAATTAAGAAGAGGAAGGGTCAAAGGAAGTGCACGACAGGGCTTGCATGAAATAAGTAAGTTGAAATCAGGAATTGATGCGAGATTTATACGCGCACAAATTAACCCTCTTTTCGTCAATTGTAGTATATGTATAAGTAGGGATCACTCAAAGAATGCAAAACTAAACTTTAAAACACTAAGACAAACCAAAAGACTCAAAACAACACAAACACACTCAAATCTGCCTAAAAACCACTTTCTGGGCAGATTTGAGCACAAACACAAATTTGGACGAAATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

393

Amino Acids

45.45

Weight (kDa)

10.18

Isoelectric Point (pI)

48.43

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 398
AccB7I CCANNNNNTGG 1 cut(s) 1138
AccII CGCG 2 cut(s) 506, 990
AciI CCGC 2 cut(s) 506, 726
AclI AACGTT 2 cut(s) 129, 694
AclWI GGATC 2 cut(s) 99, 1047
AcsI RAATTY 3 cut(s) 253, 493, 1163
AfaI GTAC 1 cut(s) 661
AfiI CCNNNNNNNGG 1 cut(s) 1138
AgsI TTSAA 5 cut(s) 164, 382, 498, 614, 961
Alw21I GWGCWC 2 cut(s) 931, 1155
Alw44I GTGCAC 1 cut(s) 927
AlwI GGATC 2 cut(s) 99, 1047
AoxI GGCC 1 cut(s) 701
ApaLI GTGCAC 1 cut(s) 927
ApeKI GCWGC 2 cut(s) 723, 833
ApoI RAATTY 3 cut(s) 253, 493, 1163
AseI ATTAAT 1 cut(s) 852
AspA2I CCTAGG 1 cut(s) 877
AspLEI GCGC 2 cut(s) 399, 992
AspS9I GGNCC 2 cut(s) 101, 290
AsuC2I CCSGG 1 cut(s) 105
AsuHPI GGTGA 2 cut(s) 755, 815
AvaII GGWCC 2 cut(s) 101, 290
AvrII CCTAGG 1 cut(s) 877
BaeGI GKGCMC 1 cut(s) 931
BbsI GAAGAC 1 cut(s) 560
Bbv12I GWGCWC 2 cut(s) 931, 1155
BbvI GCAGC 2 cut(s) 710, 820
BccI CCATC 4 cut(s) 265, 269, 382, 745
BceAI ACGGC 1 cut(s) 781
BcnI CCSGG 1 cut(s) 105
BfaI CTAG 3 cut(s) 516, 717, 878
BisI GCNGC 3 cut(s) 724, 727, 834
BlnI CCTAGG 1 cut(s) 877
BlsI GCNGC 3 cut(s) 725, 728, 835
Bme1390I CCNGG 1 cut(s) 105
Bme18I GGWCC 2 cut(s) 101, 290
BmgT120I GGNCC 2 cut(s) 101, 290
BmrFI CCNGG 1 cut(s) 105
BmrI ACTGGG 1 cut(s) 187
BmsI GCATC 5 cut(s) 54, 70, 346, 575, 964
BmuI ACTGGG 1 cut(s) 187
BpiI GAAGAC 1 cut(s) 560
BpuMI CCSGG 1 cut(s) 105
Bsa29I ATCGAT 1 cut(s) 326
BsaAI YACGTR 1 cut(s) 437
BsaJI CCNNGG 2 cut(s) 104, 877
Bsc4I CCNNNNNNNGG 1 cut(s) 1138
Bse1I ACTGG 1 cut(s) 182
BseCI ATCGAT 1 cut(s) 326
BseDI CCNNGG 2 cut(s) 104, 877
BseGI GGATG 7 cut(s) 85, 280, 393, 566, 756, 760, 819
BseLI CCNNNNNNNGG 1 cut(s) 1138
BseNI ACTGG 1 cut(s) 182
BseSI GKGCMC 1 cut(s) 931
BseXI GCAGC 2 cut(s) 710, 820
Bsh1236I CGCG 2 cut(s) 506, 990
BshFI GGCC 1 cut(s) 703
BshVI ATCGAT 1 cut(s) 326
BsiHKAI GWGCWC 2 cut(s) 931, 1155
BsiSI CCGG 1 cut(s) 104
BslFI GGGAC 2 cut(s) 414, 776
BslI CCNNNNNNNGG 1 cut(s) 1138
BsmFI GGGAC 2 cut(s) 414, 776
BsmI GAATGC 2 cut(s) 399, 1057
BsnI GGCC 1 cut(s) 703
Bsp1286I GDGCHC 2 cut(s) 931, 1155
Bsp143I GATC 2 cut(s) 91, 1039
BspACI CCGC 2 cut(s) 506, 726
BspANI GGCC 1 cut(s) 703
BspDI ATCGAT 1 cut(s) 326
BspFNI CGCG 2 cut(s) 506, 990
BspPI GGATC 2 cut(s) 99, 1047
BsrI ACTGG 1 cut(s) 182
BssECI CCNNGG 2 cut(s) 104, 877
BssMI GATC 2 cut(s) 91, 1039
BssT1I CCWWGG 1 cut(s) 877
Bst6I CTCTTC 1 cut(s) 901
BstBAI YACGTR 1 cut(s) 437
BstC8I GCNNGC 1 cut(s) 941
BstDEI CTNAG 2 cut(s) 558, 1076
BstF5I GGATG 7 cut(s) 85, 280, 393, 566, 756, 760, 819
BstFNI CGCG 2 cut(s) 506, 990
BstHHI GCGC 2 cut(s) 399, 992
BstKTI GATC 2 cut(s) 94, 1042
BstMBI GATC 2 cut(s) 91, 1039
BstSCI CCNGG 1 cut(s) 103
BstSLI GKGCMC 1 cut(s) 931
BstUI CGCG 2 cut(s) 506, 990
BstV1I GCAGC 2 cut(s) 710, 820
BstV2I GAAGAC 1 cut(s) 560
Bsu15I ATCGAT 1 cut(s) 326
BsuRI GGCC 1 cut(s) 703
BsuTUI ATCGAT 1 cut(s) 326
BtsCI GGATG 7 cut(s) 85, 280, 393, 566, 756, 760, 819
Cac8I GCNNGC 1 cut(s) 941
CfoI GCGC 2 cut(s) 399, 992
Cfr13I GGNCC 2 cut(s) 101, 290
ClaI ATCGAT 1 cut(s) 326
Csp6I GTAC 1 cut(s) 660
CviAII CATG 5 cut(s) 305, 476, 550, 688, 944
CviJI RGCY 5 cut(s) 24, 119, 703, 833, 939
CviKI_1 RGCY 5 cut(s) 24, 119, 703, 833, 939
CviQI GTAC 1 cut(s) 660
DdeI CTNAG 2 cut(s) 558, 1076
DpnI GATC 2 cut(s) 93, 1041
DpnII GATC 2 cut(s) 91, 1039
DraI TTTAAA 4 cut(s) 124, 199, 652, 1069
Eam1104I CTCTTC 1 cut(s) 901
EarI CTCTTC 1 cut(s) 901
Eco130I CCWWGG 1 cut(s) 877
Eco147I AGGCCT 1 cut(s) 703
Eco47I GGWCC 2 cut(s) 101, 290
EcoRI GAATTC 1 cut(s) 493
EcoT14I CCWWGG 1 cut(s) 877
EcoT22I ATGCAT 2 cut(s) 551, 568
ErhI CCWWGG 1 cut(s) 877
FaeI CATG 5 cut(s) 308, 479, 553, 691, 947
FaqI GGGAC 2 cut(s) 414, 776
FatI CATG 5 cut(s) 304, 475, 549, 687, 943
Fnu4HI GCNGC 3 cut(s) 724, 727, 834
FokI GGATG 7 cut(s) 92, 287, 400, 553, 763, 767, 826
Fsp4HI GCNGC 3 cut(s) 724, 727, 834
FspAI RTGCGCAY 1 cut(s) 398
FspBI CTAG 3 cut(s) 516, 717, 878
FspI TGCGCA 1 cut(s) 398
GlaI GCGC 2 cut(s) 398, 991
GluI GCNGC 3 cut(s) 724, 727, 834
HaeIII GGCC 1 cut(s) 703
HapII CCGG 1 cut(s) 104
HhaI GCGC 2 cut(s) 399, 992
Hin1II CATG 5 cut(s) 308, 479, 553, 691, 947
Hin6I GCGC 2 cut(s) 397, 990
HinP1I GCGC 2 cut(s) 397, 990
HincII GTYRAC 1 cut(s) 184
HindII GTYRAC 1 cut(s) 184
HinfI GANTC 7 cut(s) 140, 149, 169, 185, 217, 323, 1092
HpaII CCGG 1 cut(s) 104
HphI GGTGA 2 cut(s) 755, 815
Hpy166II GTNNAC 4 cut(s) 133, 184, 662, 929
Hpy188I TCNGA 1 cut(s) 897
Hpy188III TCNNGA 3 cut(s) 99, 864, 966
Hpy8I GTNNAC 4 cut(s) 133, 184, 662, 929
HpyAV CCTTC 4 cut(s) 533, 717, 740, 906
HpyCH4IV ACGT 3 cut(s) 129, 436, 694
HpyCH4V TGCA 9 cut(s) 83, 549, 566, 619, 683, 836, 929, 943, 1055
HpyF3I CTNAG 2 cut(s) 558, 1076
HpySE526I ACGT 3 cut(s) 129, 436, 694
Hsp92II CATG 5 cut(s) 308, 479, 553, 691, 947
HspAI GCGC 2 cut(s) 397, 990
Kzo9I GATC 2 cut(s) 91, 1039
LpnPI CCDG 9 cut(s) 5, 84, 117, 143, 163, 849, 920, 951, 1124
Lsp1109I GCAGC 2 cut(s) 710, 820
LweI GCATC 5 cut(s) 54, 70, 346, 575, 964
MaeI CTAG 3 cut(s) 516, 717, 878
MaeII ACGT 3 cut(s) 129, 436, 694
MaeIII GTNAC 2 cut(s) 461, 511
MalI GATC 2 cut(s) 93, 1041
MboI GATC 2 cut(s) 91, 1039
MboII GAAGA 4 cut(s) 16, 565, 808, 918
MfeI CAATTG 4 cut(s) 335, 457, 641, 1015
MhlI GDGCHC 2 cut(s) 931, 1155
MlyI GAGTC 5 cut(s) 134, 143, 163, 179, 1086
MnlI CCTC 7 cut(s) 108, 281, 714, 721, 837, 902, 1014
Mph1103I ATGCAT 2 cut(s) 551, 568
MspI CCGG 1 cut(s) 104
MspR9I CCNGG 1 cut(s) 105
MssI GTTTAAAC 1 cut(s) 652
MunI CAATTG 4 cut(s) 335, 457, 641, 1015
Mva1269I GAATGC 2 cut(s) 399, 1057
MvnI CGCG 2 cut(s) 506, 990
NciI CCSGG 1 cut(s) 105
NdeII GATC 2 cut(s) 91, 1039
NlaIII CATG 5 cut(s) 308, 479, 553, 691, 947
NmuCI GTSAC 1 cut(s) 511
NsbI TGCGCA 1 cut(s) 398
NsiI ATGCAT 2 cut(s) 551, 568
PceI AGGCCT 1 cut(s) 703
PctI GAATGC 2 cut(s) 399, 1057
PfeI GAWTC 2 cut(s) 217, 323
PflMI CCANNNNNTGG 1 cut(s) 1138
PkrI GCNGC 3 cut(s) 725, 728, 835
PleI GAGTC 5 cut(s) 134, 143, 163, 179, 1086
PmeI GTTTAAAC 1 cut(s) 652
PpsI GAGTC 5 cut(s) 134, 143, 163, 179, 1086
Ppu21I YACGTR 1 cut(s) 437
PshBI ATTAAT 1 cut(s) 852
Psp1406I AACGTT 2 cut(s) 129, 694
PspPI GGNCC 2 cut(s) 101, 290
PsrI GAACNNNNNNTAC 2 cut(s) 79, 111
RsaI GTAC 1 cut(s) 661
RsaNI GTAC 1 cut(s) 660
SatI GCNGC 3 cut(s) 724, 727, 834
Sau3AI GATC 2 cut(s) 91, 1039
Sau96I GGNCC 2 cut(s) 101, 290
SchI GAGTC 5 cut(s) 134, 143, 163, 179, 1086
ScrFI CCNGG 1 cut(s) 105
SduI GDGCHC 2 cut(s) 931, 1155
SfaNI GCATC 5 cut(s) 54, 70, 346, 575, 964
SinI GGWCC 2 cut(s) 101, 290
SseBI AGGCCT 1 cut(s) 703
SsiI CCGC 2 cut(s) 506, 726
SspMI CTAG 3 cut(s) 516, 717, 878
StuI AGGCCT 1 cut(s) 703
StyD4I CCNGG 1 cut(s) 103
StyI CCWWGG 1 cut(s) 877
TaiI ACGT 3 cut(s) 132, 439, 697
TaqI TCGA 2 cut(s) 326, 539
TatI WGTACW 1 cut(s) 659
TauI GCSGC 1 cut(s) 729
TfiI GAWTC 2 cut(s) 217, 323
TseFI GTSAC 1 cut(s) 511
TseI GCWGC 2 cut(s) 723, 833
Tsp45I GTSAC 1 cut(s) 511
Van91I CCANNNNNTGG 1 cut(s) 1138
VneI GTGCAC 1 cut(s) 927
VpaK11BI GGWCC 2 cut(s) 101, 290
VspI ATTAAT 1 cut(s) 852
XapI RAATTY 3 cut(s) 253, 493, 1163
XmaJI CCTAGG 1 cut(s) 877
XspI CTAG 3 cut(s) 516, 717, 878
Zsp2I ATGCAT 2 cut(s) 551, 568
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.