pycom05g08250

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr5
OG0000608
Physical Location & Seq
Forward (+)
10927475 .. 10928871
1397 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom05g08250.5

Sequence Viewer

Length: 894 bp
ATGGGTGTTAGGCTAGTTAGAGGTTTCTTCTCCACACATGATACATATCAGATTTCCCTAATTTCATTGAATTGGACTCAGCGACGCAATCAAATTATTCTTATCAAATCATTAAGTTCTATGGAAATCATAAGCATTGACATGGCATTCGTAACTATGAAAAGCATGATACTTTTGCCAGGAATTTACTTAACACGGTCCCCTTATATTTTAGCATCAAATTCATGCATGCAAACTAAGTATCACATTCATATTTTACGAAACAATAATTGGATGAAATCAATTCATATCACATATATGTTCATGGCCTTGAATTCTCCTCTAGCTGAAACGAGTTTAGCTCCTTATAATACACCTAAGAACTTTCCAACAATCCAAACTTTGAATGGCAGGCACAGCTCCAAGGGTCTCCTCTCCTTTTCTCCTTGCAAGGCTGCGAGACAAGGGTGTTTGTGTATGAAATTAGGTTATGGTCAGAGATGGATTAGTGTTTTGAATTGTGTTGAAGGATGGATGAATGAAACCCTAATTAATTTTGGAGAAGGATTTAATCCAAGAGGAAAAAGGGAAATAGGATGCGGCAAGATCCTAGAGAGAAAGGGAGAGGGATTTGCGGCAAGGGAAAGGGCTTCTAGAAGAAAAGGTACGGCTCTCTTTAAGAATGGGATTAGGCCTTCTAGAATCATTTATTTAGGTTTTGGCTCATGTTTCCTTCTTTCTTTGTTGAGCTCCAAATCTTCTTTTTCAAATTCGTCCATCCACCTTACTCCATGCATGTGTTATCCATTCCAAGCCCAAAACTGCTCTAAAATGCTCCAAATTGCCTTTTCTTGCCACTTTAGCCAATTGAACCTACAAATACACGAAAATAGCTTAAATCACTATAATAAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

298

Amino Acids

33.71

Weight (kDa)

9.88

Isoelectric Point (pI)

52.29

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 348
AciI CCGC 2 cut(s) 579, 614
AclWI GGATC 1 cut(s) 580
AcsI RAATTY 4 cut(s) 183, 220, 313, 748
AfaI GTAC 1 cut(s) 646
AgsI TTSAA 7 cut(s) 70, 313, 385, 496, 506, 747, 850
AjnI CCWGG 1 cut(s) 178
AjuI GAANNNNNNNTTGG 2 cut(s) 253, 285
AluBI AGCT 5 cut(s) 326, 341, 399, 729, 873
AluI AGCT 5 cut(s) 326, 341, 399, 729, 873
Alw21I GWGCWC 1 cut(s) 731
Alw26I GTCTC 2 cut(s) 413, 433
AlwI GGATC 1 cut(s) 580
AoxI GGCC 2 cut(s) 306, 671
ApeKI GCWGC 1 cut(s) 434
ApoI RAATTY 4 cut(s) 183, 220, 313, 748
ArsI GACNNNNNNTTYG 2 cut(s) 131, 163
AseI ATTAAT 1 cut(s) 531
AspS9I GGNCC 1 cut(s) 198
AvaII GGWCC 1 cut(s) 198
BanII GRGCYC 1 cut(s) 731
BarI GAAGNNNNNNTAC 2 cut(s) 628, 660
Bbv12I GWGCWC 1 cut(s) 731
BbvI GCAGC 1 cut(s) 421
BccI CCATC 3 cut(s) 474, 504, 764
BceAI ACGGC 1 cut(s) 663
BciT130I CCWGG 1 cut(s) 180
BcoDI GTCTC 2 cut(s) 413, 433
BfaI CTAG 5 cut(s) 14, 323, 590, 633, 678
BisI GCNGC 3 cut(s) 435, 580, 615
BlsI GCNGC 3 cut(s) 436, 581, 616
Bme1390I CCNGG 1 cut(s) 180
Bme18I GGWCC 1 cut(s) 198
BmgT120I GGNCC 1 cut(s) 198
BmiI GGNNCC 1 cut(s) 200
BmrFI CCNGG 1 cut(s) 180
BmsI GCATC 2 cut(s) 224, 566
BplI GAGNNNNNCTC 2 cut(s) 325, 357
BsaBI GATNNNNATC 1 cut(s) 45
BsaI GGTCTC 1 cut(s) 413
BsaJI CCNNGG 1 cut(s) 402
Bse8I GATNNNNATC 1 cut(s) 45
BseBI CCWGG 1 cut(s) 180
BseDI CCNNGG 1 cut(s) 402
BseGI GGATG 5 cut(s) 279, 515, 519, 581, 756
BseJI GATNNNNATC 1 cut(s) 45
BseMII CTCAG 1 cut(s) 92
BseRI GAGGAG 2 cut(s) 309, 401
BseXI GCAGC 1 cut(s) 421
BshFI GGCC 2 cut(s) 308, 673
BsiHKAI GWGCWC 1 cut(s) 731
BslFI GGGAC 1 cut(s) 184
BsmAI GTCTC 2 cut(s) 413, 433
BsmFI GGGAC 1 cut(s) 184
BsmI GAATGC 1 cut(s) 146
BsnI GGCC 2 cut(s) 308, 673
Bso31I GGTCTC 1 cut(s) 413
Bsp1286I GDGCHC 1 cut(s) 731
Bsp143I GATC 1 cut(s) 585
BspACI CCGC 2 cut(s) 579, 614
BspANI GGCC 2 cut(s) 308, 673
BspCNI CTCAG 1 cut(s) 91
BspLI GGNNCC 1 cut(s) 200
BspPI GGATC 1 cut(s) 580
BspTNI GGTCTC 1 cut(s) 413
BssECI CCNNGG 1 cut(s) 402
BssMI GATC 1 cut(s) 585
BssT1I CCWWGG 1 cut(s) 402
Bst2UI CCWGG 1 cut(s) 180
Bst4CI ACNGT 1 cut(s) 198
BstC8I GCNNGC 2 cut(s) 230, 392
BstDEI CTNAG 3 cut(s) 78, 237, 357
BstF5I GGATG 5 cut(s) 279, 515, 519, 581, 756
BstKTI GATC 1 cut(s) 588
BstMAI GTCTC 2 cut(s) 413, 433
BstMBI GATC 1 cut(s) 585
BstMWI GCNNNNNNNGC 2 cut(s) 396, 840
BstNI CCWGG 1 cut(s) 180
BstNSI RCATGY 2 cut(s) 232, 778
BstSCI CCNGG 1 cut(s) 178
BstV1I GCAGC 1 cut(s) 421
BstX2I RGATCY 1 cut(s) 585
BstYI RGATCY 1 cut(s) 585
BsuRI GGCC 2 cut(s) 308, 673
BtsCI GGATG 5 cut(s) 279, 515, 519, 581, 756
Cac8I GCNNGC 2 cut(s) 230, 392
Cfr13I GGNCC 1 cut(s) 198
CseI GACGC 1 cut(s) 93
Csp6I GTAC 1 cut(s) 645
CviAII CATG 9 cut(s) 38, 142, 166, 225, 229, 304, 705, 771, 775
CviQI GTAC 1 cut(s) 645
DdeI CTNAG 3 cut(s) 78, 237, 357
DpnI GATC 1 cut(s) 587
DpnII GATC 1 cut(s) 585
Ecl136II GAGCTC 1 cut(s) 729
Eco130I CCWWGG 1 cut(s) 402
Eco147I AGGCCT 1 cut(s) 673
Eco24I GRGCYC 1 cut(s) 731
Eco31I GGTCTC 1 cut(s) 413
Eco47I GGWCC 1 cut(s) 198
Eco53kI GAGCTC 1 cut(s) 729
EcoICRI GAGCTC 1 cut(s) 729
EcoRI GAATTC 1 cut(s) 313
EcoRII CCWGG 1 cut(s) 178
EcoT14I CCWWGG 1 cut(s) 402
EcoT22I ATGCAT 2 cut(s) 230, 776
EcoT38I GRGCYC 1 cut(s) 731
ErhI CCWWGG 1 cut(s) 402
FaeI CATG 9 cut(s) 41, 145, 169, 228, 232, 307, 708, 774, 778
FaqI GGGAC 1 cut(s) 184
FatI CATG 9 cut(s) 37, 141, 165, 224, 228, 303, 704, 770, 774
Fnu4HI GCNGC 3 cut(s) 435, 580, 615
FokI GGATG 5 cut(s) 286, 522, 526, 588, 743
FriOI GRGCYC 1 cut(s) 731
Fsp4HI GCNGC 3 cut(s) 435, 580, 615
FspBI CTAG 5 cut(s) 14, 323, 590, 633, 678
GluI GCNGC 3 cut(s) 435, 580, 615
HaeIII GGCC 2 cut(s) 308, 673
HgaI GACGC 1 cut(s) 93
Hin1II CATG 9 cut(s) 41, 145, 169, 228, 232, 307, 708, 774, 778
HinfI GANTC 2 cut(s) 76, 681
Hpy188I TCNGA 2 cut(s) 51, 477
Hpy188III TCNNGA 2 cut(s) 633, 678
Hpy99I CGWCG 1 cut(s) 87
HpyAV CCTTC 4 cut(s) 500, 536, 684, 722
HpyCH4III ACNGT 1 cut(s) 198
HpyCH4V TGCA 4 cut(s) 228, 232, 429, 774
HpyF10VI GCNNNNNNNGC 2 cut(s) 396, 840
HpyF3I CTNAG 3 cut(s) 78, 237, 357
Hsp92II CATG 9 cut(s) 41, 145, 169, 228, 232, 307, 708, 774, 778
Kzo9I GATC 1 cut(s) 585
LmnI GCTCC 4 cut(s) 346, 404, 734, 819
LpnPI CCDG 3 cut(s) 165, 192, 376
Lsp1109I GCAGC 1 cut(s) 421
LweI GCATC 2 cut(s) 224, 566
MaeI CTAG 5 cut(s) 14, 323, 590, 633, 678
MaeIII GTNAC 1 cut(s) 151
MalI GATC 1 cut(s) 587
MboI GATC 1 cut(s) 585
MboII GAAGA 3 cut(s) 19, 648, 729
MfeI CAATTG 1 cut(s) 845
MflI RGATCY 1 cut(s) 585
MhlI GDGCHC 1 cut(s) 731
MlyI GAGTC 1 cut(s) 70
MmeI TCCRAC 1 cut(s) 392
MnlI CCTC 5 cut(s) 14, 330, 422, 551, 598
Mph1103I ATGCAT 2 cut(s) 230, 776
MseI TTAA 6 cut(s) 113, 191, 531, 549, 657, 875
MslI CAYNNNNRTG 3 cut(s) 140, 296, 775
MspR9I CCNGG 1 cut(s) 180
MunI CAATTG 1 cut(s) 845
Mva1269I GAATGC 1 cut(s) 146
MvaI CCWGG 1 cut(s) 180
MwoI GCNNNNNNNGC 2 cut(s) 396, 840
NdeII GATC 1 cut(s) 585
NlaIII CATG 9 cut(s) 41, 145, 169, 228, 232, 307, 708, 774, 778
NlaIV GGNNCC 1 cut(s) 200
NsiI ATGCAT 2 cut(s) 230, 776
NspI RCATGY 2 cut(s) 232, 778
PaeI GCATGC 1 cut(s) 232
PceI AGGCCT 1 cut(s) 673
PctI GAATGC 1 cut(s) 146
PfeI GAWTC 1 cut(s) 681
PkrI GCNGC 3 cut(s) 436, 581, 616
PleI GAGTC 1 cut(s) 70
PpsI GAGTC 1 cut(s) 70
PshBI ATTAAT 1 cut(s) 531
PsiI TTATAA 1 cut(s) 348
Psp124BI GAGCTC 1 cut(s) 731
Psp6I CCWGG 1 cut(s) 178
PspGI CCWGG 1 cut(s) 178
PspN4I GGNNCC 1 cut(s) 200
PspPI GGNCC 1 cut(s) 198
PsuI RGATCY 1 cut(s) 585
RsaI GTAC 1 cut(s) 646
RsaNI GTAC 1 cut(s) 645
RseI CAYNNNNRTG 3 cut(s) 140, 296, 775
SacI GAGCTC 1 cut(s) 731
SaqAI TTAA 6 cut(s) 113, 191, 531, 549, 657, 875
SatI GCNGC 3 cut(s) 435, 580, 615
Sau3AI GATC 1 cut(s) 585
Sau96I GGNCC 1 cut(s) 198
SchI GAGTC 1 cut(s) 70
ScrFI CCNGG 1 cut(s) 180
SduI GDGCHC 1 cut(s) 731
SfaNI GCATC 2 cut(s) 224, 566
SinI GGWCC 1 cut(s) 198
SmiMI CAYNNNNRTG 3 cut(s) 140, 296, 775
SphI GCATGC 1 cut(s) 232
SseBI AGGCCT 1 cut(s) 673
SsiI CCGC 2 cut(s) 579, 614
SspMI CTAG 5 cut(s) 14, 323, 590, 633, 678
SstI GAGCTC 1 cut(s) 731
StuI AGGCCT 1 cut(s) 673
StyD4I CCNGG 1 cut(s) 178
StyI CCWWGG 1 cut(s) 402
TaaI ACNGT 1 cut(s) 198
TauI GCSGC 2 cut(s) 582, 617
TfiI GAWTC 1 cut(s) 681
Tru1I TTAA 6 cut(s) 113, 191, 531, 549, 657, 875
Tru9I TTAA 6 cut(s) 113, 191, 531, 549, 657, 875
TseI GCWGC 1 cut(s) 434
VpaK11BI GGWCC 1 cut(s) 198
VspI ATTAAT 1 cut(s) 531
XapI RAATTY 4 cut(s) 183, 220, 313, 748
XbaI TCTAGA 2 cut(s) 632, 677
XceI RCATGY 2 cut(s) 232, 778
XcmI CCANNNNNNNNNTGG 1 cut(s) 383
XspI CTAG 5 cut(s) 14, 323, 590, 633, 678
Zsp2I ATGCAT 2 cut(s) 230, 776
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.