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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AflIII | ACRYGT | 1 cut(s) | 95 |
| AleI | CACNNNNGTG | 1 cut(s) | 196 |
| Alw26I | GTCTC | 1 cut(s) | 160 |
| BccI | CCATC | 1 cut(s) | 48 |
| BcoDI | GTCTC | 1 cut(s) | 160 |
| BmsI | GCATC | 1 cut(s) | 34 |
| BpuEI | CTTGAG | 1 cut(s) | 46 |
| BsaJI | CCNNGG | 1 cut(s) | 176 |
| Bse3DI | GCAATG | 1 cut(s) | 20 |
| BseDI | CCNNGG | 1 cut(s) | 176 |
| BseMI | GCAATG | 1 cut(s) | 20 |
| BsmAI | GTCTC | 1 cut(s) | 160 |
| BsmI | GAATGC | 1 cut(s) | 143 |
| BsrDI | GCAATG | 1 cut(s) | 20 |
| BssECI | CCNNGG | 1 cut(s) | 176 |
| BssT1I | CCWWGG | 1 cut(s) | 176 |
| BstMAI | GTCTC | 1 cut(s) | 160 |
| BstNSI | RCATGY | 1 cut(s) | 99 |
| BtgZI | GCGATG | 1 cut(s) | 11 |
| CviAII | CATG | 3 cut(s) | 83, 96, 163 |
| Eco130I | CCWWGG | 1 cut(s) | 176 |
| EcoT14I | CCWWGG | 1 cut(s) | 176 |
| EcoT22I | ATGCAT | 1 cut(s) | 6 |
| ErhI | CCWWGG | 1 cut(s) | 176 |
| FaeI | CATG | 3 cut(s) | 86, 99, 166 |
| FaiI | YATR | 9 cut(s) | 6, 73, 75, 84, 97, 119, 155, 164, 173 |
| FatI | CATG | 3 cut(s) | 82, 95, 162 |
| FspEI | CC | 9 cut(s) | 26, 33, 53, 65, 82, 83, 125, 163, 189 |
| Hin1II | CATG | 3 cut(s) | 86, 99, 166 |
| HinfI | GANTC | 1 cut(s) | 168 |
| Hpy188I | TCNGA | 1 cut(s) | 45 |
| HpyCH4V | TGCA | 2 cut(s) | 4, 25 |
| Hsp92II | CATG | 3 cut(s) | 86, 99, 166 |
| LweI | GCATC | 1 cut(s) | 34 |
| MluCI | AATT | 1 cut(s) | 14 |
| MlyI | GAGTC | 1 cut(s) | 162 |
| Mph1103I | ATGCAT | 1 cut(s) | 6 |
| MseI | TTAA | 2 cut(s) | 110, 188 |
| MslI | CAYNNNNRTG | 2 cut(s) | 152, 196 |
| Mva1269I | GAATGC | 1 cut(s) | 143 |
| NlaIII | CATG | 3 cut(s) | 86, 99, 166 |
| NsiI | ATGCAT | 1 cut(s) | 6 |
| NspI | RCATGY | 1 cut(s) | 99 |
| OliI | CACNNNNGTG | 1 cut(s) | 196 |
| PciI | ACATGT | 1 cut(s) | 95 |
| PctI | GAATGC | 1 cut(s) | 143 |
| PleI | GAGTC | 1 cut(s) | 162 |
| PpsI | GAGTC | 1 cut(s) | 162 |
| PscI | ACATGT | 1 cut(s) | 95 |
| RseI | CAYNNNNRTG | 2 cut(s) | 152, 196 |
| SaqAI | TTAA | 2 cut(s) | 110, 188 |
| SchI | GAGTC | 1 cut(s) | 162 |
| SfaNI | GCATC | 1 cut(s) | 34 |
| SgeI | CNNG | 6 cut(s) | 75, 95, 108, 151, 175, 189 |
| SmiMI | CAYNNNNRTG | 2 cut(s) | 152, 196 |
| SmlI | CTYRAG | 1 cut(s) | 61 |
| SmoI | CTYRAG | 1 cut(s) | 61 |
| Sse9I | AATT | 1 cut(s) | 14 |
| StyI | CCWWGG | 1 cut(s) | 176 |
| TasI | AATT | 1 cut(s) | 14 |
| Tru1I | TTAA | 2 cut(s) | 110, 188 |
| Tru9I | TTAA | 2 cut(s) | 110, 188 |
| TspDTI | ATGAA | 4 cut(s) | 90, 134, 136, 170 |
| XceI | RCATGY | 1 cut(s) | 99 |
| Zsp2I | ATGCAT | 1 cut(s) | 6 |