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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AccI | GTMKAC | 1 cut(s) | 244 |
| AcsI | RAATTY | 2 cut(s) | 220, 295 |
| AjuI | GAANNNNNNNTTGG | 2 cut(s) | 59, 91 |
| AleI | CACNNNNGTG | 1 cut(s) | 440 |
| ApoI | RAATTY | 2 cut(s) | 220, 295 |
| ArsI | GACNNNNNNTTYG | 2 cut(s) | 226, 258 |
| AspS9I | GGNCC | 1 cut(s) | 24 |
| AsuHPI | GGTGA | 2 cut(s) | 17, 291 |
| AsuII | TTCGAA | 2 cut(s) | 293, 375 |
| AvaII | GGWCC | 1 cut(s) | 24 |
| BbsI | GAAGAC | 1 cut(s) | 399 |
| BccI | CCATC | 1 cut(s) | 383 |
| BfaI | CTAG | 4 cut(s) | 14, 212, 344, 438 |
| Bme18I | GGWCC | 1 cut(s) | 24 |
| BmgT120I | GGNCC | 1 cut(s) | 24 |
| BmiI | GGNNCC | 1 cut(s) | 25 |
| BmsI | GCATC | 2 cut(s) | 59, 224 |
| BpiI | GAAGAC | 1 cut(s) | 399 |
| Bpu14I | TTCGAA | 2 cut(s) | 293, 375 |
| BpuEI | CTTGAG | 1 cut(s) | 64 |
| BsaBI | GATNNNNATC | 1 cut(s) | 114 |
| BsaJI | CCNNGG | 1 cut(s) | 384 |
| Bse8I | GATNNNNATC | 1 cut(s) | 114 |
| BseDI | CCNNGG | 1 cut(s) | 384 |
| BseGI | GGATG | 1 cut(s) | 394 |
| BseJI | GATNNNNATC | 1 cut(s) | 114 |
| BsmI | GAATGC | 1 cut(s) | 161 |
| Bsp119I | TTCGAA | 2 cut(s) | 293, 375 |
| BspLI | GGNNCC | 1 cut(s) | 25 |
| BspT104I | TTCGAA | 2 cut(s) | 293, 375 |
| BssECI | CCNNGG | 1 cut(s) | 384 |
| BssT1I | CCWWGG | 1 cut(s) | 384 |
| BstBI | TTCGAA | 2 cut(s) | 293, 375 |
| BstC8I | GCNNGC | 2 cut(s) | 230, 430 |
| BstF5I | GGATG | 1 cut(s) | 394 |
| BstNSI | RCATGY | 1 cut(s) | 232 |
| BstV2I | GAAGAC | 1 cut(s) | 399 |
| BtsCI | GGATG | 1 cut(s) | 394 |
| Cac8I | GCNNGC | 2 cut(s) | 230, 430 |
| Cfr13I | GGNCC | 1 cut(s) | 24 |
| CviAII | CATG | 5 cut(s) | 38, 225, 229, 286, 329 |
| CviJI | RGCY | 2 cut(s) | 13, 290 |
| CviKI_1 | RGCY | 2 cut(s) | 13, 290 |
| Eco130I | CCWWGG | 1 cut(s) | 384 |
| Eco47I | GGWCC | 1 cut(s) | 24 |
| EcoO109I | RGGNCCY | 1 cut(s) | 24 |
| EcoRI | GAATTC | 1 cut(s) | 295 |
| EcoT14I | CCWWGG | 1 cut(s) | 384 |
| EcoT22I | ATGCAT | 1 cut(s) | 230 |
| ErhI | CCWWGG | 1 cut(s) | 384 |
| FaeI | CATG | 5 cut(s) | 41, 228, 232, 289, 332 |
| FatI | CATG | 5 cut(s) | 37, 224, 228, 285, 328 |
| FauNDI | CATATG | 1 cut(s) | 91 |
| FblI | GTMKAC | 1 cut(s) | 244 |
| FokI | GGATG | 1 cut(s) | 401 |
| FspBI | CTAG | 4 cut(s) | 14, 212, 344, 438 |
| Hin1II | CATG | 5 cut(s) | 41, 228, 232, 289, 332 |
| HphI | GGTGA | 2 cut(s) | 17, 291 |
| Hpy166II | GTNNAC | 1 cut(s) | 245 |
| Hpy8I | GTNNAC | 1 cut(s) | 245 |
| HpyAV | CCTTC | 3 cut(s) | 37, 426, 430 |
| HpyCH4V | TGCA | 4 cut(s) | 50, 228, 232, 400 |
| Hsp92II | CATG | 5 cut(s) | 41, 228, 232, 289, 332 |
| LweI | GCATC | 2 cut(s) | 59, 224 |
| MaeI | CTAG | 4 cut(s) | 14, 212, 344, 438 |
| MboII | GAAGA | 3 cut(s) | 78, 399, 402 |
| MluCI | AATT | 5 cut(s) | 68, 220, 234, 295, 317 |
| MnlI | CCTC | 3 cut(s) | 14, 312, 391 |
| Mph1103I | ATGCAT | 1 cut(s) | 230 |
| MseI | TTAA | 3 cut(s) | 105, 128, 237 |
| MslI | CAYNNNNRTG | 3 cut(s) | 170, 362, 440 |
| Mva1269I | GAATGC | 1 cut(s) | 161 |
| NdeI | CATATG | 1 cut(s) | 91 |
| NlaIII | CATG | 5 cut(s) | 41, 228, 232, 289, 332 |
| NlaIV | GGNNCC | 1 cut(s) | 25 |
| NsiI | ATGCAT | 1 cut(s) | 230 |
| NspI | RCATGY | 1 cut(s) | 232 |
| NspV | TTCGAA | 2 cut(s) | 293, 375 |
| OliI | CACNNNNGTG | 1 cut(s) | 440 |
| PaeI | GCATGC | 1 cut(s) | 232 |
| PctI | GAATGC | 1 cut(s) | 161 |
| PpuMI | RGGWCCY | 1 cut(s) | 24 |
| Psp5II | RGGWCCY | 1 cut(s) | 24 |
| PspN4I | GGNNCC | 1 cut(s) | 25 |
| PspPI | GGNCC | 1 cut(s) | 24 |
| PspPPI | RGGWCCY | 1 cut(s) | 24 |
| RseI | CAYNNNNRTG | 3 cut(s) | 170, 362, 440 |
| SaqAI | TTAA | 3 cut(s) | 105, 128, 237 |
| Sau96I | GGNCC | 1 cut(s) | 24 |
| SetI | ASST | 2 cut(s) | 304, 345 |
| SfaNI | GCATC | 2 cut(s) | 59, 224 |
| SfuI | TTCGAA | 2 cut(s) | 293, 375 |
| SinI | GGWCC | 1 cut(s) | 24 |
| SmiMI | CAYNNNNRTG | 3 cut(s) | 170, 362, 440 |
| SmlI | CTYRAG | 1 cut(s) | 79 |
| SmoI | CTYRAG | 1 cut(s) | 79 |
| SphI | GCATGC | 1 cut(s) | 232 |
| Sse9I | AATT | 5 cut(s) | 68, 220, 234, 295, 317 |
| SspMI | CTAG | 4 cut(s) | 14, 212, 344, 438 |
| StyI | CCWWGG | 1 cut(s) | 384 |
| TaqI | TCGA | 2 cut(s) | 293, 375 |
| TasI | AATT | 5 cut(s) | 68, 220, 234, 295, 317 |
| Tru1I | TTAA | 3 cut(s) | 105, 128, 237 |
| Tru9I | TTAA | 3 cut(s) | 105, 128, 237 |
| TspDTI | ATGAA | 5 cut(s) | 78, 108, 154, 188, 213 |
| VpaK11BI | GGWCC | 1 cut(s) | 24 |
| XapI | RAATTY | 2 cut(s) | 220, 295 |
| XceI | RCATGY | 1 cut(s) | 232 |
| XmiI | GTMKAC | 1 cut(s) | 244 |
| XspI | CTAG | 4 cut(s) | 14, 212, 344, 438 |
| Zsp2I | ATGCAT | 1 cut(s) | 230 |