MD09G1041500.v1.1

ABC transporter B family member

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr09
OG0000317
Physical Location & Seq
Reverse (-)
2676919 .. 2684418
7500 bp
Loading structure...
UTR
Exon/CDS
Intron
MD09G1041500.v1.1.491

Sequence Viewer

Length: 3771 bp
ATGGGACAGAAAAGTCCCAGTAAGGACAATAACAGCAGCAGCACGATGAAAGTTGGGTCCATGAGGTCCATTTTCATGCATGCTGATGGGGTTGATAGATTGTTGATGATTTTAGGGCTGTTTGGATCCCTTGGTGATGGCTTCTCCACACCGCTGGTTTTGTTGATCACTAGCAAATTGATGAACAATATTGGCGGTTCACCAACTTCGGCTCAAGATGCGTTTCTGCATAACATAAATAAGAATGCAGTGGCGCTGTTGTACTTGGCTTGTGGGGGATTTGTTTGCTGTTTCCTAGAGGGATATTGCTGGACAAGAACCGGCGAGAGGCAAGCAGCTAGAATGAGAGTCAGATATTTGAAAGCAGTTCTAAGGCAAGATGTAGGCTACTTTGATTTACATGTCACAAGCACATCAGAAGTCATCACAAGTGTCTCTAATGATAGCTTAGTGATTCAAGATGTCCTAAGTGAAAAGCTACCGAACTTCGTGATGAATTCCTCCATGTTCTTTGGAAGCTACATAGCGGCGTTTATAATGATGTGGAGGCTAGCAATCGTAGGGTTTCCATTTGTGGTGCTGCTAGTAATTCCCGGTTTAATTTACGGAAGGACTTTGATGGGATTAGCTAGGCAGATTAGGGAAGAGTACAACAAGGCTGGCAACATAGCAGAGCAGGCAATTTCTTCAATCCGGACCGTTTATGCCTTCGTCGGAGAAAACAAAACTATATCGGAATTCTCTGTGGCTCTTCAGGGCTCCGTCAAGTTGGGATTGAGCCAGGGCTTGGCTAAAGGCCTGGCCATTGGAAGTAACGGCGTTGTCTTCGCCATTTGGTCTTTCATGTCGTATTATGGAAGCACAATGGTCATGTACCATAGCGCTCGGGGAGGCACTGTTTTTGCTGTTGGTGCTGCCATCGCCGTTGGTGGATTGGCATTAGGTTCTGGACTATCAAACTTGAAGTACTTTTCAGAGGCATGTTCGGCAGCCGAGCGCATAATGGAAGTGATAAGAAGAGTTCCCAAGATCGATTCCGACAACATGGAAGGCGAATGTCTAGAGGATGTCTCCGGTGAAGTCGAATTCAAGCATCTCGAATTCGCATACCCGTCGAGACCGGAAAGCATCATCTTCAAAGATTTCAACTTGACAATTCCGGCGGGCAAAACGGTGGCGTTGGTGGGCGGAAGCGGCTCGGGGAAGTCCACGGTGATATCGCTGTTGCAGCGGTTTTATGATCCACTCGGAGGCGAGGTTCTTCTTGATGGGGTGGCGATAAATAAGCTTCAGCTCAAGTGGCTGAGGTCGCAGATGGGTTTGGTGAGCCAAGAGCCTGCTCTATTTGCAACCAGCATTGAGGAGAACATACTTTTTGGGAAAGAAGATGCTGAGATTGAAGAGGTTATTGAGGCTGGAAAAGCTGCTAATGCTCATAATTTCATCTCCCAGTTGCCTCAAGGATATGATACTCAGGTTGGCGAGCGAGGTGTTCAAATGTCGGGAGGACAGAAACAAAGGATCGCCATTGCACGAGCCATCATCAAGAAACCTCGTATCCTCCTCCTAGATGAAGCCACCAGCGCACTGGACTCCGAATCCGAACGAGTAGTCCAAGAAGCCCTAGACAAAGCAGCCGTCGGCCGCACCACAATAATCATCGCGCACCGCCTCTCCACCATCCGAAATGCGGACGTGATTGCCGTAGTTCAAAACGGCCAGGTCATGGAAATTGGCTCCCACGATGAGCTCTCCCAACGTGAAAACGGCCACTACACGTCCCTTGTCCGCCTCCAACGAACGGAGAAAGAAAAGGAACCAGAAGAGTTAGGGCATTATGGTGTTTCGTCTTCCATTTCAAACGATATTCACAGCACAAGCAGCCGTAGGCTCTCCATGGTGAGTCGTTCGAGTTCCGCAAACTCGTTTGCTCAGGGTAGAGCTCCGGATCAAGAGAGTGTTGTAGAGTATGAACAGAAGAAACTGCCCGTGCCTTCGTTTAGGAGACTTTTGGCCTTGAACCTTCCCGAGTGGAAACAAGCAGTTATGGGGTGCACGAGTGCGACTCTTTTTGGGGCGGTACAGCCGTTGTACGCGTTTGCGATGGGGTCCATGATATCGGTTTATTTCTTGAAGGACCACGACGAGATTAAGGCGAAGACAAGGACTTATTCTCTTTGCTTTCTAGGGTTGGCAATCTTCTCGTTGCTGATCAATGTCAGCCAGCATTACAACTTTGCTTACATGGGAGAGTACTTGACTAAGAGGATTAGAGAGAGGATGCTTTCAAAGGTACTCACTTTTGAAGTGGGATGGTTTGATCAGGATGAGAATTCTAGTGGTGCCATTTGCTCTAGACTCGCCAAAGATGCCAATGTGGTGAGATCTTTGGTGGGTGATCGAATGGCTCTTGTTGTACAAACCATGTCAGCAGTAACAGTAGCTTGCACAATGGGACTGATCATTACATGGAGACTTGCACTTGTCATGATAGCCGTACAACCCCTCATCATTGTATGCTTCTACACAAGGCGTGTCCTGCTCAAAAATATGTCCCAGAAGGCCATAAAATCCCAGGAAGAAAGCAGCAAGCTCGCCGCGGAAGCAGTCTCCAACCTCCGGACAGTCACCGCCTTCTCATCCCAAAACCGCCTCTTGAAAATGCTCGAAAAGGCCCAAGAAGGCCCGCGTCGGGAGAGTATCCGACAGTCATGGTATGCCGGCATTGGACTCGCTTGTTCCCAAAGCCTCACGACCGTCACTTGGGCCTTCGACTTTTGGTACGGAGGCAAGCTTGTCGCCAAGGGCTACGTCAATGCAAAACAACTTTTCCAGACCTTCATGATCTTGGTCAGCACGGGCCGGGTCATTGCCGATGCTGGTAGCATGACCACAGACCTCGCCAAGGGAGCAGACGCCGTTGGGTCTGTTTTTGCCGTGTTAGACAAGTACACAAAAATCGAGCCTGAAGACCCCGAAGGTCTCGACCCCAAAAGAATTACGGGCAGCATTGAGCTACGCAACGTGCATTTCGCGTACCCCGCTAGGCCCGATGTTATGATCTTCAACGGCTTCTCAATCAAAATCGAGGCGGGAAAATCAACGGCATTGGTGGGCCAAAGTGGTTCAGGTAAATCAACGATCATCGGATTGATAGAACGGTTTTACGATCCGATCAAGGGAGCGGTCAAAATCGACGGTCAAGATGTGAAGTCGTACCACCTCAAATCATTGAGAAAACACATAGCATTGGTCAGTCAAGAGCCAACGTTATTTGCCGGGACCATACGAGAAAACATTGTGTACGGCGTATCGGACAAGGTCGATGAGTTGGAGATAGTGGAAGCCGCGAGGGCGGCCAACGCGCACGATTTCATTGCGGGATTGAAAGACGGATATGACACGTCGTGCGGGGACAGGGGAGTGCAGCTATCCGGCGGACAAAAGCAACGGATTGCTATAGCCAGGGCGATACTTCGAAACCCCGTCATTTTGTTGCTGGACGAGGCGACCAGCGCGCTCGACAGTCGGTCTGAAAAGGTTGTGCAGGACGCGCTGGAGCGTGTGATGGTGGGGAGGACCAGCGTGGTGGTGGCCCACAGGTTGAGTACAATACAGAATTGTGATATGATTACTGTGCTGGATAAAGGAAAGGTCGTGGAGAAAGGGACCCACTCGTCGCTTTTGGCCAAAGGGCCTGCAGGTGCTTATTTCTCATTGGTCAGCCTCCAAAGGACCCCACCCGCAGAAAAGGGCACTGAATTGTATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0016020 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
ko02010 ko04976 ko05206 ko05226 map02010 map04976 map05206 map05226
Pfam Domains
Protein Families
ABC_membrane ABC_tran

Protein Analysis

1257

Amino Acids

137.08

Weight (kDa)

8.67

Isoelectric Point (pI)

43.16

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ABC_membrane PF00664 36 - 303 1.2e-47 ABC transporter transmembrane region
ABC_tran PF00005 380 - 528 2.2e-33 ABC transporter
ABC_membrane PF00664 684 - 951 1.1e-50 ABC transporter transmembrane region
ABC_tran PF00005 1023 - 1172 3.1e-33 ABC transporter
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000317)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G28345 AT3G28360 AT3G28380 AT3G28390 AT3G28415 AT3G28415
fragaria_vesca FvH4_1g06060 FvH4_3g21880 FvH4_4g25260 FvH4_6g49750 FvH4_6g49750 FvH4_6g49750 FvH4_6g49750 FvH4_6g49760
malus_domestica MD00G1221200.v1.1 MD02G1065200.v1.1 MD03G1211200.v1.1 MD09G1041500.v1.1 MD09G1041600.v1.1 MD09G1041900.v1.1 MD15G1196000.v1.1 MD16G1105600.v1.1 MD17G1042800.v1.1
prunus_persica Prupe.1G241000_v2.0.a1 Prupe.3G275900_v2.0.a1 Prupe.3G276100_v2.0.a1 Prupe.3G276100_v2.0.a1 Prupe.4G204300_v2.0.a1 Prupe.7G220300_v2.0.a1
pyrus_communis pycom111g03260 pycom16g08980 pycom17g03840
rosa_chinensis RchiOBHm_Chr2g0091821 RchiOBHm_Chr2g0169781 RchiOBHm_Chr2g0169791 RchiOBHm_Chr2g0169801 RchiOBHm_Chr2g0169841 RchiOBHm_Chr2g0169851 RchiOBHm_Chr2g0169861 RchiOBHm_Chr4g0432501 RchiOBHm_Chr5g0037681 RchiOBHm_Chr5g0037691
rosa_laevigata RLG00000006836 RLG00000016254 RLG00000021903 RLG00000021904 RLG00000021905 RLG00000021906 RLG00000021907 RLG00000021908 RLG00000033801
rosa_multiflora Rmu_sc0001084.1_g000003 Rmu_sc0021402.1_g000003 Rmu_ssc0000389.1_g000016 Rmu_ssc0000389.1_g000019 Rmu_ssc0000389.1_g000024 Rmu_ssc0000389.1_g000027
rosa_roxburghii Rroxscaffold_1G00043250 Rroxscaffold_2G00081770 Rroxscaffold_2G00081780 Rroxscaffold_2G00081790 Rroxscaffold_2G00149710 Rroxscaffold_5G00373960
rosa_rugosa Rorug02G0021300 Rorug02G0543400.1 Rorug02G0543500.1 Rorug02G0543600.1 Rorug02G0543700.1 Rorug02G0543800 Rorug02G0543900 Rorug02G0544000 Rorug04G0259900.1 Rorug05G0166300 Rorug05G0166300
rosa_samantha Rh2AG065900 Rh2AG615300 Rh2AG615400 Rh2AG615500 Rh2AG615600 Rh2AG615700 Rh2AG615800 Rh2AG616000 Rh2AG616200 Rh2BG627600 Rh2BG627700 Rh2BG627800 Rh2BG627900 Rh2BG628000 Rh2BG628100 Rh2CG596900 Rh2CG597000 Rh2CG597100 Rh2CG597200 Rh2DG065000 Rh2DG638400 Rh2DG638600 Rh2DG638700 Rh2DG638800 Rh2DG638900 Rh2DG639000 Rh4AG314700 Rh4BG322400 Rh4CG337600 Rh4DG318300 Rh5AG255800 Rh5BG257900 Rh5CG291300
rosa_wichuraiana Rw0G002540 Rw2G005480 Rw2G051010 Rw2G051020 Rw2G051030 Rw2G051040 Rw4G027360 Rw5G023710 Rw5G024000

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 536
AarI CACCTGC 1 cut(s) 3692
AasI GACNNNNNNGTC 2 cut(s) 12, 3676
Acc36I ACCTGC 1 cut(s) 3692
AccB1I GGYRCC 1 cut(s) 2342
AccB7I CCANNNNNTGG 2 cut(s) 787, 1726
AccBSI CCGCTC 1 cut(s) 3185
AccII CGCG 9 cut(s) 1664, 2096, 2600, 2689, 3035, 3350, 3365, 3518, 3554
AccIII TCCGGA 3 cut(s) 693, 1945, 2619
AclI AACGTT 1 cut(s) 3269
AclWI GGATC 6 cut(s) 120, 133, 1235, 1529, 1956, 3164
AcoI YGGCCR 6 cut(s) 801, 1642, 1717, 1768, 3357, 3687
AcsI RAATTY 5 cut(s) 496, 737, 1085, 1100, 2332
AcuI CTGAAG 3 cut(s) 737, 1274, 2988
AcyI GRCGYC 1 cut(s) 2916
AdeI CACNNNGTG 1 cut(s) 3408
AfeI AGCGCT 1 cut(s) 883
AflIII ACRYGT 4 cut(s) 400, 1776, 2094, 3402
AhdI GACNNNNNGTC 1 cut(s) 3529
AjiI CACGTC 3 cut(s) 1696, 1779, 3405
AjnI CCWGG 5 cut(s) 780, 798, 1719, 2574, 3464
AloI GAACNNNNNNTCC 4 cut(s) 1242, 1274, 1596, 1628
Alw21I GWGCWC 3 cut(s) 1752, 1945, 2057
Alw26I GTCTC 7 cut(s) 439, 1075, 1111, 1999, 2467, 2614, 2987
Alw44I GTGCAC 1 cut(s) 2053
AlwI GGATC 6 cut(s) 120, 133, 1235, 1529, 1956, 3164
Ama87I CYCGRG 3 cut(s) 885, 1198, 2027
Aor13HI TCCGGA 3 cut(s) 693, 1945, 2619
Aor51HI AGCGCT 1 cut(s) 883
ApaLI GTGCAC 1 cut(s) 2053
ApoI RAATTY 5 cut(s) 496, 737, 1085, 1100, 2332
ArsI GACNNNNNNTTYG 4 cut(s) 1596, 1623, 1628, 1655
AspLEI GCGC 9 cut(s) 256, 884, 999, 1586, 1666, 3367, 3518, 3520, 3556
AsuC2I CCSGG 3 cut(s) 594, 2864, 3280
AsuHPI GGTGA 9 cut(s) 146, 192, 1088, 1225, 1336, 1912, 2392, 2408, 2620
AsuII TTCGAA 1 cut(s) 3478
AsuNHI GCTAGC 1 cut(s) 550
AvaI CYCGRG 3 cut(s) 885, 1198, 2027
AvaII GGWCC 9 cut(s) 57, 66, 696, 2109, 2137, 3282, 3579, 3669, 3735
BaeGI GKGCMC 2 cut(s) 2057, 3758
BaeI ACNNNNGTAYC 4 cut(s) 1461, 1494, 2072, 2105
BalI TGGCCA 2 cut(s) 803, 3689
BamHI GGATCC 1 cut(s) 125
BanI GGYRCC 1 cut(s) 2342
BanII GRGCYC 3 cut(s) 761, 1752, 1945
BauI CACGAG 2 cut(s) 1533, 2056
BbsI GAAGAC 4 cut(s) 817, 1842, 2165, 2976
Bbv12I GWGCWC 3 cut(s) 1752, 1945, 2057
BbvCI CCTCAGC 1 cut(s) 1304
BciT130I CCWGG 5 cut(s) 782, 800, 1721, 2576, 3466
BciVI GTATCC 2 cut(s) 1568, 2711
BclI TGATCA 4 cut(s) 165, 2211, 2320, 2460
BcnI CCSGG 3 cut(s) 594, 2864, 3280
BcoDI GTCTC 7 cut(s) 439, 1075, 1111, 1999, 2467, 2614, 2987
BfmI CTRYAG 2 cut(s) 3459, 3699
BfoI RGCGCY 2 cut(s) 257, 885
BfuAI ACCTGC 1 cut(s) 3692
BfuI GTATCC 2 cut(s) 1568, 2711
BglI GCCNNNNNGGC 1 cut(s) 3353
BglII AGATCT 1 cut(s) 2384
BmcAI AGTACT 2 cut(s) 968, 2255
Bme1390I CCNGG 8 cut(s) 594, 782, 800, 1721, 2576, 2864, 3280, 3466
Bme18I GGWCC 9 cut(s) 57, 66, 696, 2109, 2137, 3282, 3579, 3669, 3735
BmeRI GACNNNNNGTC 1 cut(s) 3529
BmeT110I CYCGRG 3 cut(s) 885, 1198, 2027
BmgBI CACGTC 3 cut(s) 1696, 1779, 3405
BmrFI CCNGG 8 cut(s) 594, 782, 800, 1721, 2576, 2864, 3280, 3466
BmrI ACTGGG 2 cut(s) 12, 1444
BmsI GCATC 7 cut(s) 208, 1102, 1137, 1378, 2271, 2359, 2866
BmtI GCTAGC 1 cut(s) 554
BmuI ACTGGG 2 cut(s) 12, 1444
BpiI GAAGAC 4 cut(s) 817, 1842, 2165, 2976
BplI GAGNNNNNCTC 4 cut(s) 1055, 1087, 2050, 2082
BpmI CTGGAG 1 cut(s) 3578
Bpu10I CCTNAGC 2 cut(s) 1304, 1932
Bpu14I TTCGAA 1 cut(s) 3478
BpuEI CTTGAG 3 cut(s) 198, 1280, 1443
BpuMI CCSGG 3 cut(s) 594, 2864, 3280
Bsa29I ATCGAT 1 cut(s) 1032
BsaHI GRCGYC 1 cut(s) 2916
BsaI GGTCTC 2 cut(s) 1111, 2987
BsaJI CCNNGG 9 cut(s) 130, 781, 1209, 1896, 2574, 2598, 2802, 2904, 3465
BsaWI WCCGGW 5 cut(s) 693, 1073, 1120, 1945, 2619
BsaXI ACNNNNNCTCC 6 cut(s) 882, 912, 1242, 1272, 1658, 1688
Bse118I RCCGGY 2 cut(s) 320, 2720
Bse1I ACTGG 3 cut(s) 18, 1450, 1593
Bse3DI GCAATG 3 cut(s) 1527, 2868, 3375
BseAI TCCGGA 3 cut(s) 693, 1945, 2619
BseBI CCWGG 5 cut(s) 782, 800, 1721, 2576, 3466
BseCI ATCGAT 1 cut(s) 1032
BseDI CCNNGG 9 cut(s) 130, 781, 1209, 1896, 2574, 2598, 2802, 2904, 3465
BseGI GGATG 6 cut(s) 1072, 1680, 2286, 2318, 2332, 2639
BseMI GCAATG 3 cut(s) 1527, 2868, 3375
BseMII CTCAG 4 cut(s) 1295, 1383, 1487, 1946
BseNI ACTGG 3 cut(s) 18, 1450, 1593
BsePI GCGCGC 1 cut(s) 3516
BseRI GAGGAG 2 cut(s) 1376, 1553
BseSI GKGCMC 2 cut(s) 2057, 3758
BseX3I CGGCCG 1 cut(s) 1642
BsgI GTGCAG 2 cut(s) 3446, 3566
Bsh1236I CGCG 9 cut(s) 1664, 2096, 2600, 2689, 3035, 3350, 3365, 3518, 3554
Bsh1285I CGRYCG 2 cut(s) 1645, 2757
BshNI GGYRCC 1 cut(s) 2342
BshVI ATCGAT 1 cut(s) 1032
BsiEI CGRYCG 2 cut(s) 1645, 2757
BsiHKAI GWGCWC 3 cut(s) 1752, 1945, 2057
BsiHKCI CYCGRG 3 cut(s) 885, 1198, 2027
BslFI GGGAC 7 cut(s) 18, 1765, 2469, 2539, 3295, 3428, 3682
BsmAI GTCTC 7 cut(s) 439, 1075, 1111, 1999, 2467, 2614, 2987
BsmFI GGGAC 7 cut(s) 18, 1765, 2469, 2539, 3295, 3428, 3682
BsmI GAATGC 1 cut(s) 250
Bso31I GGTCTC 2 cut(s) 1111, 2987
BsoBI CYCGRG 3 cut(s) 885, 1198, 2027
Bsp119I TTCGAA 1 cut(s) 3478
Bsp1286I GDGCHC 5 cut(s) 761, 1752, 1945, 2057, 3758
Bsp13I TCCGGA 3 cut(s) 693, 1945, 2619
Bsp1407I TGTACA 1 cut(s) 2416
Bsp19I CCATGG 1 cut(s) 1896
BspCNI CTCAG 4 cut(s) 1296, 1384, 1486, 1945
BspDI ATCGAT 1 cut(s) 1032
BspEI TCCGGA 3 cut(s) 693, 1945, 2619
BspFNI CGCG 9 cut(s) 1664, 2096, 2600, 2689, 3035, 3350, 3365, 3518, 3554
BspHI TCATGA 2 cut(s) 2487, 2841
BspMAI CTGCAG 1 cut(s) 3703
BspMI ACCTGC 1 cut(s) 3692
BspOI GCTAGC 1 cut(s) 554
BspPI GGATC 6 cut(s) 120, 133, 1235, 1529, 1956, 3164
BspQI GCTCTTC 1 cut(s) 756
BspT104I TTCGAA 1 cut(s) 3478
BspT107I GGYRCC 1 cut(s) 2342
BspTNI GGTCTC 2 cut(s) 1111, 2987
BsrBI CCGCTC 1 cut(s) 3185
BsrDI GCAATG 3 cut(s) 1527, 2868, 3375
BsrFI RCCGGY 2 cut(s) 320, 2720
BsrGI TGTACA 1 cut(s) 2416
BsrI ACTGG 3 cut(s) 18, 1450, 1593
BssAI RCCGGY 2 cut(s) 320, 2720
BssECI CCNNGG 9 cut(s) 130, 781, 1209, 1896, 2574, 2598, 2802, 2904, 3465
BssHII GCGCGC 1 cut(s) 3516
BssNI GRCGYC 1 cut(s) 2916
BssSI CACGAG 2 cut(s) 1533, 2056
BssT1I CCWWGG 4 cut(s) 130, 1896, 2802, 2904
Bst2BI CACGAG 2 cut(s) 1533, 2056
Bst2UI CCWGG 5 cut(s) 782, 800, 1721, 2576, 3466
Bst6I CTCTTC 5 cut(s) 639, 756, 1012, 1395, 1818
BstACI GRCGYC 1 cut(s) 2916
BstAUI TGTACA 1 cut(s) 2416
BstBI TTCGAA 1 cut(s) 3478
BstDEI CTNAG 8 cut(s) 371, 448, 467, 1304, 1392, 1473, 1932, 2262
BstDSI CCRYGG 3 cut(s) 1209, 1896, 2598
BstENI CCTNNNNNAGG 1 cut(s) 2903
BstF5I GGATG 6 cut(s) 1072, 1680, 2286, 2318, 2332, 2639
BstFNI CGCG 9 cut(s) 1664, 2096, 2600, 2689, 3035, 3350, 3365, 3518, 3554
BstH2I RGCGCY 2 cut(s) 257, 885
BstHHI GCGC 9 cut(s) 256, 884, 999, 1586, 1666, 3367, 3518, 3520, 3556
BstMAI GTCTC 7 cut(s) 439, 1075, 1111, 1999, 2467, 2614, 2987
BstMCI CGRYCG 2 cut(s) 1645, 2757
BstNI CCWGG 5 cut(s) 782, 800, 1721, 2576, 3466
BstNSI RCATGY 3 cut(s) 83, 404, 984
BstSCI CCNGG 8 cut(s) 592, 780, 798, 1719, 2574, 2862, 3278, 3464
BstSFI CTRYAG 2 cut(s) 3459, 3699
BstSLI GKGCMC 2 cut(s) 2057, 3758
BstUI CGCG 9 cut(s) 1664, 2096, 2600, 2689, 3035, 3350, 3365, 3518, 3554
BstV2I GAAGAC 4 cut(s) 817, 1842, 2165, 2976
BstX2I RGATCY 2 cut(s) 125, 2384
BstXI CCANNNNNNTGG 3 cut(s) 154, 1588, 3589
BstYI RGATCY 2 cut(s) 125, 2384
BstZI CGGCCG 1 cut(s) 1642
Bsu15I ATCGAT 1 cut(s) 1032
BsuI GTATCC 2 cut(s) 1568, 2711
BsuTUI ATCGAT 1 cut(s) 1032
BtgI CCRYGG 3 cut(s) 1209, 1896, 2598
BtgZI GCGATG 3 cut(s) 904, 1645, 2117
BtrI CACGTC 3 cut(s) 1696, 1779, 3405
BtsCI GGATG 6 cut(s) 1072, 1680, 2286, 2318, 2332, 2639
BtsI GCAGTG 1 cut(s) 255
BtsIMutI CAGTG 4 cut(s) 255, 894, 1586, 3756
BveI ACCTGC 1 cut(s) 3692
CciI TCATGA 2 cut(s) 2487, 2841
CfoI GCGC 9 cut(s) 256, 884, 999, 1586, 1666, 3367, 3518, 3520, 3556
Cfr10I RCCGGY 2 cut(s) 320, 2720
Cfr42I CCGCGG 1 cut(s) 2601
ClaI ATCGAT 1 cut(s) 1032
CpoI CGGWCCG 1 cut(s) 696
CseI GACGC 3 cut(s) 2678, 2924, 3560
CspI CGGWCCG 1 cut(s) 696
DdeI CTNAG 8 cut(s) 371, 448, 467, 1304, 1392, 1473, 1932, 2262
DraIII CACNNNGTG 1 cut(s) 3408
DrdI GACNNNNNNGTC 2 cut(s) 12, 3676
DriI GACNNNNNGTC 1 cut(s) 3529
DseDI GACNNNNNNGTC 2 cut(s) 12, 3676
EaeI YGGCCR 6 cut(s) 801, 1642, 1717, 1768, 3357, 3687
EagI CGGCCG 1 cut(s) 1642
Eam1104I CTCTTC 5 cut(s) 639, 756, 1012, 1395, 1818
Eam1105I GACNNNNNGTC 1 cut(s) 3529
EarI CTCTTC 5 cut(s) 639, 756, 1012, 1395, 1818
EciI GGCGGA 3 cut(s) 1203, 1778, 3453
Ecl136II GAGCTC 2 cut(s) 1750, 1943
EclXI CGGCCG 1 cut(s) 1642
Eco130I CCWWGG 4 cut(s) 130, 1896, 2802, 2904
Eco147I AGGCCT 1 cut(s) 798
Eco24I GRGCYC 3 cut(s) 761, 1752, 1945
Eco31I GGTCTC 2 cut(s) 1111, 2987
Eco32I GATATC 2 cut(s) 1218, 2118
Eco47I GGWCC 9 cut(s) 57, 66, 696, 2109, 2137, 3282, 3579, 3669, 3735
Eco47III AGCGCT 1 cut(s) 883
Eco52I CGGCCG 1 cut(s) 1642
Eco53kI GAGCTC 2 cut(s) 1750, 1943
Eco57I CTGAAG 3 cut(s) 737, 1274, 2988
Eco88I CYCGRG 3 cut(s) 885, 1198, 2027
EcoICRI GAGCTC 2 cut(s) 1750, 1943
EcoNI CCTNNNNNAGG 1 cut(s) 2903
EcoO109I RGGNCCY 3 cut(s) 3669, 3695, 3735
EcoRI GAATTC 5 cut(s) 496, 737, 1085, 1100, 2332
EcoRII CCWGG 5 cut(s) 780, 798, 1719, 2574, 3464
EcoRV GATATC 2 cut(s) 1218, 2118
EcoT14I CCWWGG 4 cut(s) 130, 1896, 2802, 2904
EcoT22I ATGCAT 1 cut(s) 81
EcoT38I GRGCYC 3 cut(s) 761, 1752, 1945
ErhI CCWWGG 4 cut(s) 130, 1896, 2802, 2904
FaqI GGGAC 7 cut(s) 18, 1765, 2469, 2539, 3295, 3428, 3682
FauI CCCGC 7 cut(s) 1156, 2694, 3049, 3085, 3373, 3404, 3751
FbaI TGATCA 4 cut(s) 165, 2211, 2320, 2460
FokI GGATG 6 cut(s) 1079, 1667, 2293, 2325, 2339, 2626
FriOI GRGCYC 3 cut(s) 761, 1752, 1945
GlaI GCGC 9 cut(s) 255, 883, 998, 1585, 1665, 3366, 3517, 3519, 3555
GsuI CTGGAG 1 cut(s) 3578
HaeII RGCGCY 2 cut(s) 257, 885
HgaI GACGC 3 cut(s) 2678, 2924, 3560
HhaI GCGC 9 cut(s) 256, 884, 999, 1586, 1666, 3367, 3518, 3520, 3556
Hin1I GRCGYC 1 cut(s) 2916
Hin6I GCGC 9 cut(s) 254, 882, 997, 1584, 1664, 3365, 3516, 3518, 3554
HinP1I GCGC 9 cut(s) 254, 882, 997, 1584, 1664, 3365, 3516, 3518, 3554
HindIII AAGCTT 2 cut(s) 1286, 2792
HinfI GANTC 9 cut(s) 348, 454, 1034, 1592, 1598, 1903, 2065, 2358, 2730
HphI GGTGA 9 cut(s) 146, 192, 1088, 1225, 1336, 1912, 2392, 2408, 2620
Hpy166II GTNNAC 5 cut(s) 200, 1209, 2055, 2952, 3304
Hpy8I GTNNAC 5 cut(s) 200, 1209, 2055, 2952, 3304
Hpy99I CGWCG 8 cut(s) 716, 1117, 1643, 2147, 2694, 3200, 3409, 3682
HpyCH4IV ACGT 7 cut(s) 1695, 1759, 1778, 2811, 3024, 3269, 3404
HpyF3I CTNAG 8 cut(s) 371, 448, 467, 1304, 1392, 1473, 1932, 2262
HpySE526I ACGT 7 cut(s) 1695, 1759, 1778, 2811, 3024, 3269, 3404
Hsp92I GRCGYC 1 cut(s) 2916
HspAI GCGC 9 cut(s) 254, 882, 997, 1584, 1664, 3365, 3516, 3518, 3554
KflI GGGWCCC 1 cut(s) 3669
Kpn2I TCCGGA 3 cut(s) 693, 1945, 2619
KroI GCCGGC 1 cut(s) 2720
KroNI GCCGGC 1 cut(s) 2722
Ksp22I TGATCA 4 cut(s) 165, 2211, 2320, 2460
KspI CCGCGG 1 cut(s) 2601
LguI GCTCTTC 1 cut(s) 756
LmnI GCTCC 6 cut(s) 764, 1742, 1948, 2909, 3182, 3559
LweI GCATC 7 cut(s) 208, 1102, 1137, 1378, 2271, 2359, 2866
MaeII ACGT 7 cut(s) 1695, 1759, 1778, 2811, 3024, 3269, 3404
MaeIII GTNAC 5 cut(s) 403, 812, 2434, 2626, 2758
MbiI CCGCTC 1 cut(s) 3185
MflI RGATCY 2 cut(s) 125, 2384
MhlI GDGCHC 5 cut(s) 761, 1752, 1945, 2057, 3758
MlsI TGGCCA 2 cut(s) 803, 3689
MluI ACGCGT 1 cut(s) 2094
MluNI TGGCCA 2 cut(s) 803, 3689
MlyI GAGTC 6 cut(s) 357, 1586, 1912, 2059, 2352, 2724
MmeI TCCRAC 6 cut(s) 694, 1062, 1819, 2637, 2728, 3312
Mox20I TGGCCA 2 cut(s) 803, 3689
Mph1103I ATGCAT 1 cut(s) 81
MroI TCCGGA 3 cut(s) 693, 1945, 2619
MroNI GCCGGC 1 cut(s) 2720
MscI TGGCCA 2 cut(s) 803, 3689
MseI TTAA 2 cut(s) 599, 2151
MslI CAYNNNNRTG 3 cut(s) 74, 84, 1839
Msp20I TGGCCA 2 cut(s) 803, 3689
MspA1I CMGCKG 3 cut(s) 154, 1231, 2600
MspR9I CCNGG 8 cut(s) 594, 782, 800, 1721, 2576, 2864, 3280, 3466
Mva1269I GAATGC 1 cut(s) 250
MvaI CCWGG 5 cut(s) 782, 800, 1721, 2576, 3466
MvnI CGCG 9 cut(s) 1664, 2096, 2600, 2689, 3035, 3350, 3365, 3518, 3554
NaeI GCCGGC 1 cut(s) 2722
NciI CCSGG 3 cut(s) 594, 2864, 3280
NcoI CCATGG 1 cut(s) 1896
NgoMIV GCCGGC 1 cut(s) 2720
NheI GCTAGC 1 cut(s) 550
NmeAIII GCCGAG 1 cut(s) 1018
NmuCI GTSAC 3 cut(s) 403, 2626, 2758
NsiI ATGCAT 1 cut(s) 81
NspI RCATGY 3 cut(s) 83, 404, 984
NspV TTCGAA 1 cut(s) 3478
PaeI GCATGC 1 cut(s) 83
PagI TCATGA 2 cut(s) 2487, 2841
PaqCI CACCTGC 1 cut(s) 3692
PauI GCGCGC 1 cut(s) 3516
PceI AGGCCT 1 cut(s) 798
PciI ACATGT 1 cut(s) 400
PciSI GCTCTTC 1 cut(s) 756
PcsI WCGNNNNNNNCGW 1 cut(s) 1701
PctI GAATGC 1 cut(s) 250
PdiI GCCGGC 1 cut(s) 2722
PfeI GAWTC 3 cut(s) 454, 1034, 1598
PflFI GACNNNGTC 1 cut(s) 3320
PflMI CCANNNNNTGG 2 cut(s) 787, 1726
PleI GAGTC 6 cut(s) 356, 1586, 1911, 2059, 2352, 2724
PpsI GAGTC 6 cut(s) 356, 1586, 1911, 2059, 2352, 2724
PpuMI RGGWCCY 2 cut(s) 3669, 3735
PscI ACATGT 1 cut(s) 400
PsiI TTATAA 1 cut(s) 536
Psp124BI GAGCTC 2 cut(s) 1752, 1945
Psp1406I AACGTT 1 cut(s) 3269
Psp5II RGGWCCY 2 cut(s) 3669, 3735
Psp6I CCWGG 5 cut(s) 780, 798, 1719, 2574, 3464
PspGI CCWGG 5 cut(s) 780, 798, 1719, 2574, 3464
PspPPI RGGWCCY 2 cut(s) 3669, 3735
PstI CTGCAG 1 cut(s) 3703
PsuI RGATCY 2 cut(s) 125, 2384
PsyI GACNNNGTC 1 cut(s) 3320
PteI GCGCGC 1 cut(s) 3516
RseI CAYNNNNRTG 3 cut(s) 74, 84, 1839
Rsr2I CGGWCCG 1 cut(s) 696
RsrII CGGWCCG 1 cut(s) 696
SacI GAGCTC 2 cut(s) 1752, 1945
SacII CCGCGG 1 cut(s) 2601
SapI GCTCTTC 1 cut(s) 756
SaqAI TTAA 2 cut(s) 599, 2151
SbfI CCTGCAGG 1 cut(s) 3703
ScaI AGTACT 2 cut(s) 968, 2255
SchI GAGTC 6 cut(s) 357, 1586, 1912, 2059, 2352, 2724
ScrFI CCNGG 8 cut(s) 594, 782, 800, 1721, 2576, 2864, 3280, 3466
SdaI CCTGCAGG 1 cut(s) 3703
SduI GDGCHC 5 cut(s) 761, 1752, 1945, 2057, 3758
SfaNI GCATC 7 cut(s) 208, 1102, 1137, 1378, 2271, 2359, 2866
SfcI CTRYAG 2 cut(s) 3459, 3699
Sfr303I CCGCGG 1 cut(s) 2601
SfuI TTCGAA 1 cut(s) 3478
SgrBI CCGCGG 1 cut(s) 2601
SinI GGWCC 9 cut(s) 57, 66, 696, 2109, 2137, 3282, 3579, 3669, 3735
SmiMI CAYNNNNRTG 3 cut(s) 74, 84, 1839
SmlI CTYRAG 3 cut(s) 213, 1295, 1458
SmoI CTYRAG 3 cut(s) 213, 1295, 1458
SphI GCATGC 1 cut(s) 83
Sse8387I CCTGCAGG 1 cut(s) 3703
SseBI AGGCCT 1 cut(s) 798
SspI AATATT 1 cut(s) 190
SstI GAGCTC 2 cut(s) 1752, 1945
StuI AGGCCT 1 cut(s) 798
StyD4I CCNGG 8 cut(s) 592, 780, 798, 1719, 2574, 2862, 3278, 3464
StyI CCWWGG 4 cut(s) 130, 1896, 2802, 2904
TaiI ACGT 7 cut(s) 1698, 1762, 1781, 2814, 3027, 3272, 3407
TaqII GACCGA 1 cut(s) 3519
TatI WGTACW 7 cut(s) 261, 648, 966, 2253, 2416, 2949, 3608
TauI GCSGC 6 cut(s) 530, 1197, 1647, 2600, 3350, 3359
TfiI GAWTC 3 cut(s) 454, 1034, 1598
Tru1I TTAA 2 cut(s) 599, 2151
Tru9I TTAA 2 cut(s) 599, 2151
TscAI CASTG 4 cut(s) 255, 901, 1593, 3763
TseFI GTSAC 3 cut(s) 403, 2626, 2758
Tsp45I GTSAC 3 cut(s) 403, 2626, 2758
TspGWI ACGGA 6 cut(s) 621, 751, 1817, 2799, 3408, 3466
TspRI CASTG 4 cut(s) 255, 901, 1593, 3763
Tth111I GACNNNGTC 1 cut(s) 3320
Van91I CCANNNNNTGG 2 cut(s) 787, 1726
VneI GTGCAC 1 cut(s) 2053
VpaK11BI GGWCC 9 cut(s) 57, 66, 696, 2109, 2137, 3282, 3579, 3669, 3735
XagI CCTNNNNNAGG 1 cut(s) 2903
XapI RAATTY 5 cut(s) 496, 737, 1085, 1100, 2332
XbaI TCTAGA 2 cut(s) 1060, 2354
XceI RCATGY 3 cut(s) 83, 404, 984
XcmI CCANNNNNNNNNTGG 2 cut(s) 1585, 3589
ZrmI AGTACT 2 cut(s) 968, 2255
Zsp2I ATGCAT 1 cut(s) 81
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.