ABC transporter B family member
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AanI | TTATAA | 1 cut(s) | 431 |
| AarI | CACCTGC | 1 cut(s) | 1252 |
| AatII | GACGTC | 1 cut(s) | 1527 |
| Acc36I | ACCTGC | 2 cut(s) | 1252, 1540 |
| AccB1I | GGYRCC | 4 cut(s) | 137, 418, 2153, 3092 |
| AccI | GTMKAC | 2 cut(s) | 1148, 1922 |
| AccII | CGCG | 4 cut(s) | 647, 1035, 2500, 3318 |
| AccIII | TCCGGA | 2 cut(s) | 1837, 2939 |
| AclI | AACGTT | 1 cut(s) | 1008 |
| AclWI | GGATC | 4 cut(s) | 223, 1175, 2975, 3508 |
| AcoI | YGGCCR | 4 cut(s) | 112, 741, 1215, 3441 |
| AcsI | RAATTY | 2 cut(s) | 1025, 2143 |
| AcuI | CTGAAG | 1 cut(s) | 923 |
| AcyI | GRCGYC | 2 cut(s) | 138, 1524 |
| AdeI | CACNNNGTG | 1 cut(s) | 1646 |
| AfeI | AGCGCT | 1 cut(s) | 1854 |
| AfiI | CCNNNNNNNGG | 4 cut(s) | 1190, 1257, 2503, 2990 |
| AflIII | ACRYGT | 1 cut(s) | 645 |
| AgeI | ACCGGT | 1 cut(s) | 2757 |
| AhlI | ACTAGT | 1 cut(s) | 3335 |
| AjnI | CCWGG | 5 cut(s) | 234, 726, 2339, 2573, 2855 |
| AjuI | GAANNNNNNNTTGG | 2 cut(s) | 3070, 3102 |
| Alw21I | GWGCWC | 3 cut(s) | 926, 1953, 2669 |
| Alw26I | GTCTC | 6 cut(s) | 262, 1553, 1769, 2425, 2457, 3438 |
| AlwI | GGATC | 4 cut(s) | 223, 1175, 2975, 3508 |
| AlwNI | CAGNNNCTG | 2 cut(s) | 622, 1900 |
| Ama87I | CYCGRG | 2 cut(s) | 1138, 3190 |
| Aor13HI | TCCGGA | 2 cut(s) | 1837, 2939 |
| Aor51HI | AGCGCT | 1 cut(s) | 1854 |
| ApoI | RAATTY | 2 cut(s) | 1025, 2143 |
| ArsI | GACNNNNNNTTYG | 2 cut(s) | 2825, 2857 |
| AseI | ATTAAT | 1 cut(s) | 2963 |
| AsiGI | ACCGGT | 1 cut(s) | 2757 |
| Asp700I | GAANNNNTTC | 3 cut(s) | 908, 1981, 2882 |
| AspLEI | GCGC | 8 cut(s) | 6, 140, 196, 824, 1035, 1855, 3310, 3318 |
| AspS9I | GGNCC | 8 cut(s) | 1248, 2485, 2495, 2707, 2755, 2859, 2937, 3449 |
| AsuC2I | CCSGG | 2 cut(s) | 534, 2786 |
| AsuNHI | GCTAGC | 2 cut(s) | 490, 2404 |
| AvaI | CYCGRG | 2 cut(s) | 1138, 3190 |
| AvaII | GGWCC | 4 cut(s) | 1248, 2707, 2755, 2937 |
| AxyI | CCTNAGG | 1 cut(s) | 3453 |
| BaeI | ACNNNNGTAYC | 2 cut(s) | 825, 858 |
| BalI | TGGCCA | 3 cut(s) | 743, 1217, 3443 |
| BanI | GGYRCC | 4 cut(s) | 137, 418, 2153, 3092 |
| BauI | CACGAG | 2 cut(s) | 389, 1684 |
| BbsI | GAAGAC | 2 cut(s) | 1976, 2787 |
| Bbv12I | GWGCWC | 3 cut(s) | 926, 1953, 2669 |
| BceAI | ACGGC | 4 cut(s) | 772, 1698, 2732, 3133 |
| BcgI | CGANNNNNNTGC | 4 cut(s) | 742, 776, 2590, 2624 |
| BciT130I | CCWGG | 5 cut(s) | 236, 728, 2341, 2575, 2857 |
| BciVI | GTATCC | 2 cut(s) | 2357, 3200 |
| BclI | TGATCA | 1 cut(s) | 2131 |
| BcnI | CCSGG | 2 cut(s) | 534, 2786 |
| BcoDI | GTCTC | 6 cut(s) | 262, 1553, 1769, 2425, 2457, 3438 |
| BcuI | ACTAGT | 1 cut(s) | 3335 |
| BfmI | CTRYAG | 4 cut(s) | 684, 1166, 2409, 3482 |
| BfoI | RGCGCY | 3 cut(s) | 141, 197, 1856 |
| BfuAI | ACCTGC | 2 cut(s) | 1252, 1540 |
| BfuI | GTATCC | 2 cut(s) | 2357, 3200 |
| BglII | AGATCT | 1 cut(s) | 2195 |
| BlpI | GCTNAGC | 2 cut(s) | 925, 2663 |
| BmcAI | AGTACT | 2 cut(s) | 908, 2066 |
| Bme1390I | CCNGG | 7 cut(s) | 236, 534, 728, 2341, 2575, 2786, 2857 |
| Bme18I | GGWCC | 4 cut(s) | 1248, 2707, 2755, 2937 |
| BmeT110I | CYCGRG | 2 cut(s) | 1138, 3190 |
| BmgT120I | GGNCC | 8 cut(s) | 1248, 2485, 2495, 2707, 2755, 2859, 2937, 3449 |
| BmiI | GGNNCC | 8 cut(s) | 67, 139, 420, 1098, 1250, 2155, 2938, 3094 |
| BmrFI | CCNGG | 7 cut(s) | 236, 534, 728, 2341, 2575, 2786, 2857 |
| BmtI | GCTAGC | 2 cut(s) | 494, 2408 |
| BpiI | GAAGAC | 2 cut(s) | 1976, 2787 |
| BplI | GAGNNNNNCTC | 6 cut(s) | 1550, 1582, 2405, 2437, 2589, 2621 |
| BpmI | CTGGAG | 1 cut(s) | 257 |
| Bpu10I | CCTNAGC | 1 cut(s) | 3162 |
| Bpu1102I | GCTNAGC | 2 cut(s) | 925, 2663 |
| BpuEI | CTTGAG | 7 cut(s) | 127, 138, 1220, 2370, 2439, 2538, 3182 |
| BpuMI | CCSGG | 2 cut(s) | 534, 2786 |
| Bsa29I | ATCGAT | 2 cut(s) | 1426, 3006 |
| BsaHI | GRCGYC | 2 cut(s) | 138, 1524 |
| BsaI | GGTCTC | 1 cut(s) | 1769 |
| BsaJI | CCNNGG | 4 cut(s) | 865, 2574, 2613, 3060 |
| BsaWI | WCCGGW | 4 cut(s) | 1837, 2757, 2776, 2939 |
| BsaXI | ACNNNNNCTCC | 8 cut(s) | 822, 852, 1402, 1432, 1487, 1517, 3322, 3352 |
| Bsc4I | CCNNNNNNNGG | 4 cut(s) | 1190, 1257, 2503, 2990 |
| Bse118I | RCCGGY | 1 cut(s) | 2757 |
| Bse1I | ACTGG | 6 cut(s) | 115, 265, 570, 604, 859, 2148 |
| Bse21I | CCTNAGG | 1 cut(s) | 3453 |
| Bse3DI | GCAATG | 2 cut(s) | 2274, 2679 |
| BseAI | TCCGGA | 2 cut(s) | 1837, 2939 |
| BseBI | CCWGG | 5 cut(s) | 236, 728, 2341, 2575, 2857 |
| BseCI | ATCGAT | 2 cut(s) | 1426, 3006 |
| BseDI | CCNNGG | 4 cut(s) | 865, 2574, 2613, 3060 |
| BseGI | GGATG | 4 cut(s) | 1509, 1652, 2143, 3045 |
| BseLI | CCNNNNNNNGG | 4 cut(s) | 1190, 1257, 2503, 2990 |
| BseMI | GCAATG | 2 cut(s) | 2274, 2679 |
| BseNI | ACTGG | 6 cut(s) | 115, 265, 570, 604, 859, 2148 |
| BseRI | GAGGAG | 1 cut(s) | 3246 |
| BseYI | CCCAGC | 3 cut(s) | 709, 1103, 2120 |
| BsgI | GTGCAG | 1 cut(s) | 1908 |
| Bsh1236I | CGCG | 4 cut(s) | 647, 1035, 2500, 3318 |
| Bsh1285I | CGRYCG | 1 cut(s) | 1430 |
| BshNI | GGYRCC | 4 cut(s) | 137, 418, 2153, 3092 |
| BshTI | ACCGGT | 1 cut(s) | 2757 |
| BshVI | ATCGAT | 2 cut(s) | 1426, 3006 |
| BsiEI | CGRYCG | 1 cut(s) | 1430 |
| BsiHKAI | GWGCWC | 3 cut(s) | 926, 1953, 2669 |
| BsiHKCI | CYCGRG | 2 cut(s) | 1138, 3190 |
| BsiSI | CCGG | 7 cut(s) | 533, 1838, 2691, 2758, 2777, 2786, 2940 |
| BsiWI | CGTACG | 1 cut(s) | 1475 |
| BslFI | GGGAC | 6 cut(s) | 576, 947, 1234, 1485, 1787, 3207 |
| BslI | CCNNNNNNNGG | 4 cut(s) | 1190, 1257, 2503, 2990 |
| BsmAI | GTCTC | 6 cut(s) | 262, 1553, 1769, 2425, 2457, 3438 |
| BsmBI | CGTCTC | 2 cut(s) | 2457, 3438 |
| BsmFI | GGGAC | 6 cut(s) | 576, 947, 1234, 1485, 1787, 3207 |
| BsmI | GAATGC | 5 cut(s) | 190, 470, 878, 1907, 2511 |
| Bso31I | GGTCTC | 1 cut(s) | 1769 |
| BsoBI | CYCGRG | 2 cut(s) | 1138, 3190 |
| Bsp1286I | GDGCHC | 3 cut(s) | 926, 1953, 2669 |
| Bsp13I | TCCGGA | 2 cut(s) | 1837, 2939 |
| Bsp1407I | TGTACA | 1 cut(s) | 2227 |
| Bsp1720I | GCTNAGC | 2 cut(s) | 925, 2663 |
| BspDI | ATCGAT | 2 cut(s) | 1426, 3006 |
| BspEI | TCCGGA | 2 cut(s) | 1837, 2939 |
| BspFNI | CGCG | 4 cut(s) | 647, 1035, 2500, 3318 |
| BspHI | TCATGA | 3 cut(s) | 1678, 2830, 3178 |
| BspLI | GGNNCC | 8 cut(s) | 67, 139, 420, 1098, 1250, 2155, 2938, 3094 |
| BspMAI | CTGCAG | 4 cut(s) | 688, 1170, 2413, 3486 |
| BspMI | ACCTGC | 2 cut(s) | 1252, 1540 |
| BspOI | GCTAGC | 2 cut(s) | 494, 2408 |
| BspPI | GGATC | 4 cut(s) | 223, 1175, 2975, 3508 |
| BspT107I | GGYRCC | 4 cut(s) | 137, 418, 2153, 3092 |
| BspTNI | GGTCTC | 1 cut(s) | 1769 |
| BsrDI | GCAATG | 2 cut(s) | 2274, 2679 |
| BsrFI | RCCGGY | 1 cut(s) | 2757 |
| BsrGI | TGTACA | 1 cut(s) | 2227 |
| BsrI | ACTGG | 6 cut(s) | 115, 265, 570, 604, 859, 2148 |
| BssAI | RCCGGY | 1 cut(s) | 2757 |
| BssECI | CCNNGG | 4 cut(s) | 865, 2574, 2613, 3060 |
| BssNI | GRCGYC | 2 cut(s) | 138, 1524 |
| BssSI | CACGAG | 2 cut(s) | 389, 1684 |
| BssT1I | CCWWGG | 3 cut(s) | 865, 2613, 3060 |
| Bst2BI | CACGAG | 2 cut(s) | 389, 1684 |
| Bst2UI | CCWGG | 5 cut(s) | 236, 728, 2341, 2575, 2857 |
| Bst6I | CTCTTC | 1 cut(s) | 880 |
| BstACI | GRCGYC | 2 cut(s) | 138, 1524 |
| BstAPI | GCANNNNNTGC | 3 cut(s) | 1074, 1646, 3173 |
| BstAUI | TGTACA | 1 cut(s) | 2227 |
| BstF5I | GGATG | 4 cut(s) | 1509, 1652, 2143, 3045 |
| BstFNI | CGCG | 4 cut(s) | 647, 1035, 2500, 3318 |
| BstH2I | RGCGCY | 3 cut(s) | 141, 197, 1856 |
| BstHHI | GCGC | 8 cut(s) | 6, 140, 196, 824, 1035, 1855, 3310, 3318 |
| BstMAI | GTCTC | 6 cut(s) | 262, 1553, 1769, 2425, 2457, 3438 |
| BstMCI | CGRYCG | 1 cut(s) | 1430 |
| BstNI | CCWGG | 5 cut(s) | 236, 728, 2341, 2575, 2857 |
| BstNSI | RCATGY | 3 cut(s) | 344, 2365, 3124 |
| BstSCI | CCNGG | 7 cut(s) | 234, 532, 726, 2339, 2573, 2784, 2855 |
| BstSFI | CTRYAG | 4 cut(s) | 684, 1166, 2409, 3482 |
| BstUI | CGCG | 4 cut(s) | 647, 1035, 2500, 3318 |
| BstV2I | GAAGAC | 2 cut(s) | 1976, 2787 |
| BstX2I | RGATCY | 1 cut(s) | 2195 |
| BstYI | RGATCY | 1 cut(s) | 2195 |
| Bsu15I | ATCGAT | 2 cut(s) | 1426, 3006 |
| Bsu36I | CCTNAGG | 1 cut(s) | 3453 |
| BsuI | GTATCC | 2 cut(s) | 2357, 3200 |
| BsuTUI | ATCGAT | 2 cut(s) | 1426, 3006 |
| BtgZI | GCGATG | 3 cut(s) | 90, 745, 1474 |
| BtsCI | GGATG | 4 cut(s) | 1509, 1652, 2143, 3045 |
| BtsI | GCAGTG | 3 cut(s) | 195, 2306, 2439 |
| BveI | ACCTGC | 2 cut(s) | 1252, 1540 |
| CaiI | CAGNNNCTG | 2 cut(s) | 622, 1900 |
| CciI | TCATGA | 3 cut(s) | 1678, 2830, 3178 |
| CfoI | GCGC | 8 cut(s) | 6, 140, 196, 824, 1035, 1855, 3310, 3318 |
| Cfr10I | RCCGGY | 1 cut(s) | 2757 |
| Cfr13I | GGNCC | 8 cut(s) | 1248, 2485, 2495, 2707, 2755, 2859, 2937, 3449 |
| ClaI | ATCGAT | 2 cut(s) | 1426, 3006 |
| CseI | GACGC | 3 cut(s) | 158, 2724, 2735 |
| CspAI | ACCGGT | 1 cut(s) | 2757 |
| DinI | GGCGCC | 1 cut(s) | 139 |
| DraIII | CACNNNGTG | 1 cut(s) | 1646 |
| EaeI | YGGCCR | 4 cut(s) | 112, 741, 1215, 3441 |
| Eam1104I | CTCTTC | 1 cut(s) | 880 |
| EarI | CTCTTC | 1 cut(s) | 880 |
| Eco130I | CCWWGG | 3 cut(s) | 865, 2613, 3060 |
| Eco147I | AGGCCT | 1 cut(s) | 726 |
| Eco31I | GGTCTC | 1 cut(s) | 1769 |
| Eco47I | GGWCC | 4 cut(s) | 1248, 2707, 2755, 2937 |
| Eco47III | AGCGCT | 1 cut(s) | 1854 |
| Eco57I | CTGAAG | 1 cut(s) | 923 |
| Eco81I | CCTNAGG | 1 cut(s) | 3453 |
| Eco88I | CYCGRG | 2 cut(s) | 1138, 3190 |
| EcoO109I | RGGNCCY | 1 cut(s) | 3449 |
| EcoRI | GAATTC | 2 cut(s) | 1025, 2143 |
| EcoRII | CCWGG | 5 cut(s) | 234, 726, 2339, 2573, 2855 |
| EcoT14I | CCWWGG | 3 cut(s) | 865, 2613, 3060 |
| EgeI | GGCGCC | 1 cut(s) | 139 |
| EheI | GGCGCC | 1 cut(s) | 139 |
| ErhI | CCWWGG | 3 cut(s) | 865, 2613, 3060 |
| Esp3I | CGTCTC | 2 cut(s) | 2457, 3438 |
| FalI | AAGNNNNNCTT | 2 cut(s) | 2686, 2718 |
| FaqI | GGGAC | 6 cut(s) | 576, 947, 1234, 1485, 1787, 3207 |
| FauI | CCCGC | 2 cut(s) | 2505, 2524 |
| FauNDI | CATATG | 1 cut(s) | 2159 |
| FbaI | TGATCA | 1 cut(s) | 2131 |
| FblI | GTMKAC | 2 cut(s) | 1148, 1922 |
| FokI | GGATG | 4 cut(s) | 1496, 1639, 2150, 3052 |
| GlaI | GCGC | 8 cut(s) | 5, 139, 195, 823, 1034, 1854, 3309, 3317 |
| GsaI | CCCAGC | 3 cut(s) | 713, 1107, 2124 |
| GsuI | CTGGAG | 1 cut(s) | 257 |
| HaeII | RGCGCY | 3 cut(s) | 141, 197, 1856 |
| HapII | CCGG | 7 cut(s) | 533, 1838, 2691, 2758, 2777, 2786, 2940 |
| HgaI | GACGC | 3 cut(s) | 158, 2724, 2735 |
| HhaI | GCGC | 8 cut(s) | 6, 140, 196, 824, 1035, 1855, 3310, 3318 |
| Hin1I | GRCGYC | 2 cut(s) | 138, 1524 |
| Hin6I | GCGC | 8 cut(s) | 4, 138, 194, 822, 1033, 1853, 3308, 3316 |
| HinP1I | GCGC | 8 cut(s) | 4, 138, 194, 822, 1033, 1853, 3308, 3316 |
| HincII | GTYRAC | 4 cut(s) | 1039, 1149, 1923, 2026 |
| HindII | GTYRAC | 4 cut(s) | 1039, 1149, 1923, 2026 |
| HindIII | AAGCTT | 1 cut(s) | 1362 |
| HpaI | GTTAAC | 1 cut(s) | 2026 |
| HpaII | CCGG | 7 cut(s) | 533, 1838, 2691, 2758, 2777, 2786, 2940 |
| Hpy166II | GTNNAC | 9 cut(s) | 643, 1012, 1039, 1149, 1622, 1923, 1936, 2026, 2763 |
| Hpy8I | GTNNAC | 9 cut(s) | 643, 1012, 1039, 1149, 1622, 1923, 1936, 2026, 2763 |
| Hpy99I | CGWCG | 6 cut(s) | 1526, 1577, 1698, 1756, 1927, 2612 |
| HpyCH4IV | ACGT | 5 cut(s) | 1008, 1474, 1524, 1588, 2648 |
| HpySE526I | ACGT | 5 cut(s) | 1008, 1474, 1524, 1588, 2648 |
| Hsp92I | GRCGYC | 2 cut(s) | 138, 1524 |
| HspAI | GCGC | 8 cut(s) | 4, 138, 194, 822, 1033, 1853, 3308, 3316 |
| KasI | GGCGCC | 1 cut(s) | 137 |
| Kpn2I | TCCGGA | 2 cut(s) | 1837, 2939 |
| Ksp22I | TGATCA | 1 cut(s) | 2131 |
| KspAI | GTTAAC | 1 cut(s) | 2026 |
| LmnI | GCTCC | 5 cut(s) | 11, 65, 456, 1432, 1948 |
| MaeII | ACGT | 5 cut(s) | 1008, 1474, 1524, 1588, 2648 |
| MaeIII | GTNAC | 7 cut(s) | 146, 289, 326, 343, 2245, 3224, 3425 |
| MboII | GAAGA | 7 cut(s) | 897, 1337, 1352, 1781, 1981, 2101, 2792 |
| MflI | RGATCY | 1 cut(s) | 2195 |
| MhlI | GDGCHC | 3 cut(s) | 926, 1953, 2669 |
| MlsI | TGGCCA | 3 cut(s) | 743, 1217, 3443 |
| MluI | ACGCGT | 1 cut(s) | 645 |
| MluNI | TGGCCA | 3 cut(s) | 743, 1217, 3443 |
| Mly113I | GGCGCC | 1 cut(s) | 138 |
| MlyI | GAGTC | 7 cut(s) | 727, 968, 969, 1411, 2163, 3189, 3433 |
| MmeI | TCCRAC | 4 cut(s) | 230, 1609, 2448, 2733 |
| Mox20I | TGGCCA | 3 cut(s) | 743, 1217, 3443 |
| MroI | TCCGGA | 2 cut(s) | 1837, 2939 |
| MroXI | GAANNNNTTC | 3 cut(s) | 908, 1981, 2882 |
| MscI | TGGCCA | 3 cut(s) | 743, 1217, 3443 |
| MseI | TTAA | 8 cut(s) | 1086, 1221, 1788, 2025, 2562, 2963, 3044, 3359 |
| MslI | CAYNNNNRTG | 2 cut(s) | 24, 179 |
| Msp20I | TGGCCA | 3 cut(s) | 743, 1217, 3443 |
| MspA1I | CMGCKG | 5 cut(s) | 848, 1103, 1466, 1900, 2243 |
| MspI | CCGG | 7 cut(s) | 533, 1838, 2691, 2758, 2777, 2786, 2940 |
| MspR9I | CCNGG | 7 cut(s) | 236, 534, 728, 2341, 2575, 2786, 2857 |
| Mva1269I | GAATGC | 5 cut(s) | 190, 470, 878, 1907, 2511 |
| MvaI | CCWGG | 5 cut(s) | 236, 728, 2341, 2575, 2857 |
| MvnI | CGCG | 4 cut(s) | 647, 1035, 2500, 3318 |
| NarI | GGCGCC | 1 cut(s) | 138 |
| NciI | CCSGG | 2 cut(s) | 534, 2786 |
| NdeI | CATATG | 1 cut(s) | 2159 |
| NheI | GCTAGC | 2 cut(s) | 490, 2404 |
| NlaIV | GGNNCC | 8 cut(s) | 67, 139, 420, 1098, 1250, 2155, 2938, 3094 |
| NmuCI | GTSAC | 4 cut(s) | 146, 343, 2245, 3425 |
| NspI | RCATGY | 3 cut(s) | 344, 2365, 3124 |
| PagI | TCATGA | 3 cut(s) | 1678, 2830, 3178 |
| PaqCI | CACCTGC | 1 cut(s) | 1252 |
| PceI | AGGCCT | 1 cut(s) | 726 |
| PcsI | WCGNNNNNNNCGW | 3 cut(s) | 1020, 1145, 1432 |
| PctI | GAATGC | 5 cut(s) | 190, 470, 878, 1907, 2511 |
| PdmI | GAANNNNTTC | 3 cut(s) | 908, 1981, 2882 |
| PfeI | GAWTC | 5 cut(s) | 394, 1423, 1977, 2883, 3003 |
| Pfl23II | CGTACG | 1 cut(s) | 1475 |
| PflFI | GACNNNGTC | 1 cut(s) | 1927 |
| PfoI | TCCNGGA | 2 cut(s) | 234, 532 |
| PinAI | ACCGGT | 1 cut(s) | 2757 |
| Ple19I | CGATCG | 1 cut(s) | 1430 |
| PleI | GAGTC | 7 cut(s) | 726, 968, 968, 1411, 2163, 3189, 3432 |
| PluTI | GGCGCC | 1 cut(s) | 141 |
| PpsI | GAGTC | 7 cut(s) | 726, 968, 968, 1411, 2163, 3189, 3432 |
| PshBI | ATTAAT | 1 cut(s) | 2963 |
| PsiI | TTATAA | 1 cut(s) | 431 |
| Psp1406I | AACGTT | 1 cut(s) | 1008 |
| Psp6I | CCWGG | 5 cut(s) | 234, 726, 2339, 2573, 2855 |
| PspFI | CCCAGC | 3 cut(s) | 709, 1103, 2120 |
| PspGI | CCWGG | 5 cut(s) | 234, 726, 2339, 2573, 2855 |
| PspLI | CGTACG | 1 cut(s) | 1475 |
| PspN4I | GGNNCC | 8 cut(s) | 67, 139, 420, 1098, 1250, 2155, 2938, 3094 |
| PspPI | GGNCC | 8 cut(s) | 1248, 2485, 2495, 2707, 2755, 2859, 2937, 3449 |
| PstI | CTGCAG | 4 cut(s) | 688, 1170, 2413, 3486 |
| PstNI | CAGNNNCTG | 2 cut(s) | 622, 1900 |
| PsuI | RGATCY | 1 cut(s) | 2195 |
| PsyI | GACNNNGTC | 1 cut(s) | 1927 |
| PvuI | CGATCG | 1 cut(s) | 1430 |
| PvuII | CAGCTG | 3 cut(s) | 848, 1103, 2243 |
| RseI | CAYNNNNRTG | 2 cut(s) | 24, 179 |
| SalI | GTCGAC | 2 cut(s) | 1147, 1921 |
| SaqAI | TTAA | 8 cut(s) | 1086, 1221, 1788, 2025, 2562, 2963, 3044, 3359 |
| Sau96I | GGNCC | 8 cut(s) | 1248, 2485, 2495, 2707, 2755, 2859, 2937, 3449 |
| ScaI | AGTACT | 2 cut(s) | 908, 2066 |
| SchI | GAGTC | 7 cut(s) | 727, 968, 969, 1411, 2163, 3189, 3433 |
| ScrFI | CCNGG | 7 cut(s) | 236, 534, 728, 2341, 2575, 2786, 2857 |
| SduI | GDGCHC | 3 cut(s) | 926, 1953, 2669 |
| SfcI | CTRYAG | 4 cut(s) | 684, 1166, 2409, 3482 |
| SfoI | GGCGCC | 1 cut(s) | 139 |
| SinI | GGWCC | 4 cut(s) | 1248, 2707, 2755, 2937 |
| SmiMI | CAYNNNNRTG | 2 cut(s) | 24, 179 |
| SmlI | CTYRAG | 7 cut(s) | 142, 153, 1235, 2385, 2454, 2553, 3197 |
| SmoI | CTYRAG | 7 cut(s) | 142, 153, 1235, 2385, 2454, 2553, 3197 |
| SpeI | ACTAGT | 1 cut(s) | 3335 |
| SseBI | AGGCCT | 1 cut(s) | 726 |
| SspDI | GGCGCC | 1 cut(s) | 137 |
| StuI | AGGCCT | 1 cut(s) | 726 |
| StyD4I | CCNGG | 7 cut(s) | 234, 532, 726, 2339, 2573, 2784, 2855 |
| StyI | CCWWGG | 3 cut(s) | 865, 2613, 3060 |
| TaiI | ACGT | 5 cut(s) | 1011, 1477, 1527, 1591, 2651 |
| TaqII | GACCGA | 1 cut(s) | 1236 |
| TatI | WGTACW | 5 cut(s) | 201, 906, 2064, 2227, 3235 |
| TauI | GCSGC | 5 cut(s) | 117, 138, 470, 1900, 3263 |
| TfiI | GAWTC | 5 cut(s) | 394, 1423, 1977, 2883, 3003 |
| Tru1I | TTAA | 8 cut(s) | 1086, 1221, 1788, 2025, 2562, 2963, 3044, 3359 |
| Tru9I | TTAA | 8 cut(s) | 1086, 1221, 1788, 2025, 2562, 2963, 3044, 3359 |
| TseFI | GTSAC | 4 cut(s) | 146, 343, 2245, 3425 |
| Tsp45I | GTSAC | 4 cut(s) | 146, 343, 2245, 3425 |
| TspGWI | ACGGA | 7 cut(s) | 498, 561, 970, 1029, 1607, 2610, 2728 |
| Tth111I | GACNNNGTC | 1 cut(s) | 1927 |
| VpaK11BI | GGWCC | 4 cut(s) | 1248, 2707, 2755, 2937 |
| VspI | ATTAAT | 1 cut(s) | 2963 |
| XapI | RAATTY | 2 cut(s) | 1025, 2143 |
| XbaI | TCTAGA | 1 cut(s) | 2165 |
| XceI | RCATGY | 3 cut(s) | 344, 2365, 3124 |
| XcmI | CCANNNNNNNNNTGG | 1 cut(s) | 1592 |
| XmiI | GTMKAC | 2 cut(s) | 1148, 1922 |
| XmnI | GAANNNNTTC | 3 cut(s) | 908, 1981, 2882 |
| ZraI | GACGTC | 1 cut(s) | 1525 |
| ZrmI | AGTACT | 2 cut(s) | 908, 2066 |