MD17G1042800.v1.1

ABC transporter B family member

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr17
OG0000317
Physical Location & Seq
Reverse (-)
3138371 .. 3145858
7488 bp
Loading structure...
UTR
Exon/CDS
Intron
MD17G1042800.v1.1.491

Sequence Viewer

Length: 3783 bp
ATGGGACAGAAAAGCCCCAGTAATGACAACAGCAACAACAACAGTATCATGAAAGTTGGGTCCATGCGGTCCATTTTCATGCATGCTGATGGGGTTGATAGATTGCTGATGATCTTAGGGCTATTTGGATCCCTTGGTGATGGCTTTTCCACACCGTTGGTTTTGTTGATCACTAGCAAATTGATGAACAATATTGGCGGTTCACCAACTTCAGCTCAGGTTGCTTTTCTGCATAACATAAATAAGAATGCAGTGGCGCTGTTGTACTTGGCTTGTGGGTCATTTGTATGCTGTTTCCTAGAGGGATATTGCTGGACAAGAACTGGCGAGAGGCAGGCAGCTAGGATGAGAGTACGATATTTGAAAGCAGTTCTAAGGCAAGATGTGGGCTACTTTGATTTACATGTCACAAGCACCTCAGAAGTGATCACAAGTGTCTCTAATGATAGCTTAGTGATTCAAGATGTCCTAAGTGAAAAGCTACCGAACTTCTTGATGAATTTCTCCATGTTCTCCGGAAGCTACGTGGCGGCTTTTATAATGATGTGGAGGCTAGCAATCGTAGGGTTTCCATTTGTGGTGCTGCTAGTAATTCCCGGTTTAATTTACGGAAGGACTTTGATGGGATTAGCTAGGCAGATTAGGGAAGAGTACAACAAGGCTGGCAACATAGCAGAGCAGGCAATTTCTTCAATCCGCACAGTTTATGCTTTCGTCGGAGAAAACAAAACCATATCGGAATTCTCTGCGGCTCTTCAGGGCTCCGTCAGGTTGGGATTGAGCCAGGGCTTGGCTAAAGGACTGGCCATCGGAAGCAACGGCGTTGTCTTCGCCATTTGGTCTTTCATGTCTTTTTATGGAAGCAGAATGGTCATGTACCATGGCGCTCAGGGAGGCACTGTTTTTGCTGTTGGTGCTGCCATCGCCGTTGGCGGATTGGCATTAGGTTCTGGACTATCAAACTTGAAGTACTTTTCAGAGGCATGTTCGGCAGCCGAGCGCATAATGGAAGTGATAAGAAGAGTTCCCAAGATCGATTCCGACAACATGGAAGGCGAAATCTTGGAGGATGTTTCCGGTGAAGTTGAATTCAAGCACGTGGAATTCGCATACCCGTCGAGGCCGGAAAGCATTATCTTCAAAGACTTCAACCTGACAGTTCCGGCGGGCAAAACGGTGGCGTTGGTGGGCGGAAGCGGCTCGGGGAAGTCCACGGTGATAGCCCTGTTGCAGCGGTTTTATGATCCGCTCGGAGGCGAGATTCTTCTTGATGGGGTGGCGATAAATAAGCTTCAGCTCAAGTGGCTGCGATCGCAGATGGGTTTGGTGAGCCAAGAGCCTGCTCTATTTGCAACCAGCATTAAGGAGAACATACTTTTTGGGAAAGAAGATGCTGGGATTGAAGAGATTACTGAGGCTGGGAAAGCTGCTAATGCTCATAATTTCATCTCCCAGTTGCCTCAAGGATACGATACTCAGGTTGGCGAGCGGGGTGTTCAAATGTCGGGAGGACAGAAACAAAGGATCGCCATTGCACGAGCCATCATCAAGAAGCCTCGTATCCTCCTCCTAGACGAAGCCACCAGCGCACTGGACTCCGAATCCGAACGAGTAGTCCAAGAAGCCCTAGACAAAGCAGCCGTCGGCCGCACCACAATCATCATCGCGCACCGCCTCTCCACCATCCGAAACGCAGACGTGATTGCCGTAGTTCAAAACGGTCAGGTCATGGAAACCGGCTCCCACGACGAGCTCTCCCAACGCGAACATGGCCACTACACGTCCCTTGTCCGCCTCCAACAAACAGAAAAAGAAAAAGAACCGGAAGAGTTAGGGCATTACGGTGCTTCGTCTTCCATTTCAAAAGACATTCACAGCACAAGCAGCCGTAGGCACAGCACAAGCAGCCGTAGGCTCTCCATGTTGAGTCGTTCGAGTTCGGCGAACTCATTTGCTCAGGGTAGAGCTCCTGATCAAGAGAGTGTTGTAGGGTATGAACATAAGAAACTGCCCGTGCCTTCTTTTAGGAGACTTTTGGCCTTGAACCTTCCGGAGTGGAAACAAGCAGTTATGGGGTGCACGAGTGCGACTCTTTTTGGGGCAGTACAACCGCTGTACGCATTTGCCATGGGTTCCATGGTGTCGGTTTATTTCTTGACGGATCACGACAAGATTAAGGCGAAAACAAGGACTTACTCGCTTTGCTTTCTAGGGTTGGCAATCTTCTCGTTGCTGATCAATGTCAGCCAGCATTACAACTTTGCTTACATGGGAGAGTACTTGACTAAGAGGGTTAGAGAGAGGATGCTTTCAAAGGTACTCACTTTTGAAGTGGGGTGGTTTGATCAGGATGAGAATTCTAGCGGTGCCATTTGCTCTAGACTCGCCAAAGATGCCAATGTGGTGAGATCTTTGGTGGGTGATCGAATGGCTCTTGTTGTACAAACCATCTCAGCAGTAACAGTAGCTTGCACAATGGGATTGATCATTACATGGAGGCTTGCAATTGTCATGATAGCCGTACAACCCCTCATCATTGTATGCTTCTACACAAGGCGAGTCCTGCTCAAAAGCATGTCCAGGAAGGCCATAAAATCCCAGGAAGAAAGCAGCAAGCTCGCCGCGGAAGCAGTGTCCAACCTCCGGACAGTCACGGCCTTCTCATCCCAAGACCGACTATTGAAAATGCTCGAAAAGGCCCAAGAAGGCCCTCGTCGGGAGAGTATCCGACAGTCGTGGTACGCCGGCATTGGACTCGCTTGTTCCCAAAGCCTCACAACCGTCACTTGGGCCTTCGACTTTTGGTACGGAGGCAAGCTTGTTGCCAAGGGCTACGTCAAAGCCCAACAACTATTCCAGACCTTCATGATCTTGGTGAGCACAGGCCGGGTCATTGCCGATGCCGGTAGCATGACCACGGACCTCGCCAAGGGCGCAGATGCCGTCGGGTCTGTTTTCGCGGTGTTAGACCGATACACAAAAATTGAGCCTGAAGACCCGGAAGGTCTCGAGCCCGAAGCAATAACGGGTCACATTGAGCTACACGACATACATTTTGCGTACCCCGCTAGGCCCGATGTTATGATCTTCAAAGGCTTCTCAATCAAAATCGATGCGGGAAAATCAACGGCACTCGTGGGCCAAAGTGGTTCGGGTAAATCAACGATCATTGGACTGATCGAACGGTTTTATGATCCCATTAAAGGGGAAGTAAAAATCGACGGTCGAGATGTGAAGTCGTACCACCTCAAGTCGTTGAGAAAACACATAGCGTTGGTCAGCCAAGAGCCAACGTTGTTCGCCGGGACGATACGAGAAAACATTGTGTACGGCGTGTCAGATAAGGTCGACGAGTTGGAGGTGGTGGAAGCTGCAAGGGCAGCCAACGCGCACGATTTCATCGCCGGATTAAAAGATGGATACGACACGTCGTGCGGGGATAGAGGAGTGCAGTTATCCGGAGGACAAAAACAGCGGATTGCCATAGCCAGGGCGATACTTCGAAACCCTGTCGTTTTGTTGTTGGACGAGGCGACCAGCGCGCTCGACAGTCGATCGGAGAAGGTTGTGCAGGACGCGCTAGAGCGCGTGATGGTGGGGAGGACTAGTGTGGTGGTGGCCCACAGGTTGAGTACGATACAGAATTGCGATATGATTGCCGTGCTCGATAAAGGGTGGGTGGTGGAGAAGGGGACCCACTCGTCGCTTTTGGCCAAGGGTCCTACAGGTGCTTACTTCTCATTGGTCAGCCTCCAAAGAACCCCACGTCAAGAGGGCACTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0016020 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
ko02010 ko04976 ko05206 ko05226 map02010 map04976 map05206 map05226
Pfam Domains
Protein Families
ABC_membrane ABC_tran

Protein Analysis

1261

Amino Acids

137.5

Weight (kDa)

8.86

Isoelectric Point (pI)

41.0

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ABC_membrane PF00664 37 - 304 9.2e-47 ABC transporter transmembrane region
ABC_tran PF00005 381 - 529 2.3e-33 ABC transporter
ABC_membrane PF00664 692 - 959 1.5e-49 ABC transporter transmembrane region
ABC_tran PF00005 1031 - 1180 3.8e-33 ABC transporter
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000317)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G28345 AT3G28360 AT3G28380 AT3G28390 AT3G28415 AT3G28415
fragaria_vesca FvH4_1g06060 FvH4_3g21880 FvH4_4g25260 FvH4_6g49750 FvH4_6g49750 FvH4_6g49750 FvH4_6g49750 FvH4_6g49760
malus_domestica MD00G1221200.v1.1 MD02G1065200.v1.1 MD03G1211200.v1.1 MD09G1041500.v1.1 MD09G1041600.v1.1 MD09G1041900.v1.1 MD15G1196000.v1.1 MD16G1105600.v1.1 MD17G1042800.v1.1
prunus_persica Prupe.1G241000_v2.0.a1 Prupe.3G275900_v2.0.a1 Prupe.3G276100_v2.0.a1 Prupe.3G276100_v2.0.a1 Prupe.4G204300_v2.0.a1 Prupe.7G220300_v2.0.a1
pyrus_communis pycom111g03260 pycom16g08980 pycom17g03840
rosa_chinensis RchiOBHm_Chr2g0091821 RchiOBHm_Chr2g0169781 RchiOBHm_Chr2g0169791 RchiOBHm_Chr2g0169801 RchiOBHm_Chr2g0169841 RchiOBHm_Chr2g0169851 RchiOBHm_Chr2g0169861 RchiOBHm_Chr4g0432501 RchiOBHm_Chr5g0037681 RchiOBHm_Chr5g0037691
rosa_laevigata RLG00000006836 RLG00000016254 RLG00000021903 RLG00000021904 RLG00000021905 RLG00000021906 RLG00000021907 RLG00000021908 RLG00000033801
rosa_multiflora Rmu_sc0001084.1_g000003 Rmu_sc0021402.1_g000003 Rmu_ssc0000389.1_g000016 Rmu_ssc0000389.1_g000019 Rmu_ssc0000389.1_g000024 Rmu_ssc0000389.1_g000027
rosa_roxburghii Rroxscaffold_1G00043250 Rroxscaffold_2G00081770 Rroxscaffold_2G00081780 Rroxscaffold_2G00081790 Rroxscaffold_2G00149710 Rroxscaffold_5G00373960
rosa_rugosa Rorug02G0021300 Rorug02G0543400.1 Rorug02G0543500.1 Rorug02G0543600.1 Rorug02G0543700.1 Rorug02G0543800 Rorug02G0543900 Rorug02G0544000 Rorug04G0259900.1 Rorug05G0166300 Rorug05G0166300
rosa_samantha Rh2AG065900 Rh2AG615300 Rh2AG615400 Rh2AG615500 Rh2AG615600 Rh2AG615700 Rh2AG615800 Rh2AG616000 Rh2AG616200 Rh2BG627600 Rh2BG627700 Rh2BG627800 Rh2BG627900 Rh2BG628000 Rh2BG628100 Rh2CG596900 Rh2CG597000 Rh2CG597100 Rh2CG597200 Rh2DG065000 Rh2DG638400 Rh2DG638600 Rh2DG638700 Rh2DG638800 Rh2DG638900 Rh2DG639000 Rh4AG314700 Rh4BG322400 Rh4CG337600 Rh4DG318300 Rh5AG255800 Rh5BG257900 Rh5CG291300
rosa_wichuraiana Rw0G002540 Rw2G005480 Rw2G051010 Rw2G051020 Rw2G051030 Rw2G051040 Rw4G027360 Rw5G023710 Rw5G024000

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 539
AasI GACNNNNNNGTC 1 cut(s) 3700
AccB1I GGYRCC 1 cut(s) 2366
AccB7I CCANNNNNTGG 1 cut(s) 790
AccBSI CCGCTC 2 cut(s) 1249, 1489
AccI GTMKAC 1 cut(s) 3348
AccII CGCG 8 cut(s) 1667, 1764, 2624, 2960, 3389, 3542, 3578, 3588
AccIII TCCGGA 4 cut(s) 515, 2050, 2643, 3458
AclI AACGTT 1 cut(s) 3293
AclWI GGATC 6 cut(s) 123, 136, 1238, 1532, 2169, 3188
AcoI YGGCCR 4 cut(s) 804, 1645, 1771, 3711
AcsI RAATTY 5 cut(s) 499, 740, 1088, 1103, 2356
AcuI CTGAAG 4 cut(s) 195, 740, 1277, 3012
AcvI CACGTG 1 cut(s) 1099
AdeI CACNNNGTG 1 cut(s) 3432
AfiI CCNNNNNNNGG 9 cut(s) 790, 1253, 2643, 2716, 2787, 2929, 3203, 3204, 3489
AflIII ACRYGT 3 cut(s) 403, 1779, 3426
AhlI ACTAGT 1 cut(s) 3605
AjiI CACGTC 4 cut(s) 1699, 1782, 3429, 3767
AjnI CCWGG 4 cut(s) 783, 2579, 2598, 3488
AloI GAACNNNNNNTCC 2 cut(s) 1599, 1631
Alw21I GWGCWC 5 cut(s) 1755, 1969, 2081, 2882, 3666
Alw26I GTCTC 3 cut(s) 442, 2023, 3011
Alw44I GTGCAC 1 cut(s) 2077
AlwI GGATC 6 cut(s) 123, 136, 1238, 1532, 2169, 3188
Ama87I CYCGRG 2 cut(s) 1201, 3008
Aor13HI TCCGGA 4 cut(s) 515, 2050, 2643, 3458
ApaLI GTGCAC 1 cut(s) 2077
ApoI RAATTY 5 cut(s) 499, 740, 1088, 1103, 2356
ArsI GACNNNNNNTTYG 6 cut(s) 1599, 1626, 1631, 1658, 3038, 3070
AsiSI GCGATCGC 1 cut(s) 1313
AsuC2I CCSGG 4 cut(s) 597, 2888, 2999, 3304
AsuHPI GGTGA 8 cut(s) 149, 195, 1091, 1228, 1339, 2416, 2432, 2887
AsuII TTCGAA 1 cut(s) 3502
AsuNHI GCTAGC 1 cut(s) 553
AvaI CYCGRG 2 cut(s) 1201, 3008
AvaII GGWCC 5 cut(s) 60, 69, 2920, 3693, 3719
BaeGI GKGCMC 2 cut(s) 2081, 3779
BaeI ACNNNNGTAYC 4 cut(s) 28, 61, 1464, 1497
BalI TGGCCA 3 cut(s) 806, 1773, 3713
BamHI GGATCC 1 cut(s) 128
BanI GGYRCC 1 cut(s) 2366
BanII GRGCYC 4 cut(s) 764, 1755, 1969, 3015
BauI CACGAG 3 cut(s) 1536, 2080, 3134
BbrPI CACGTG 1 cut(s) 1099
BbsI GAAGAC 3 cut(s) 820, 1845, 3000
Bbv12I GWGCWC 5 cut(s) 1755, 1969, 2081, 2882, 3666
BciT130I CCWGG 4 cut(s) 785, 2581, 2600, 3490
BciVI GTATCC 4 cut(s) 1460, 1571, 2735, 3413
BclI TGATCA 6 cut(s) 168, 426, 1972, 2235, 2344, 2484
BcnI CCSGG 4 cut(s) 597, 2888, 2999, 3304
BcoDI GTCTC 3 cut(s) 442, 2023, 3011
BcuI ACTAGT 1 cut(s) 3605
BfmI CTRYAG 1 cut(s) 3723
BfoI RGCGCY 2 cut(s) 260, 888
BfuI GTATCC 4 cut(s) 1460, 1571, 2735, 3413
BglII AGATCT 1 cut(s) 2408
BmcAI AGTACT 2 cut(s) 971, 2279
Bme1390I CCNGG 8 cut(s) 597, 785, 2581, 2600, 2888, 2999, 3304, 3490
Bme18I GGWCC 5 cut(s) 60, 69, 2920, 3693, 3719
BmeT110I CYCGRG 2 cut(s) 1201, 3008
BmgBI CACGTC 4 cut(s) 1699, 1782, 3429, 3767
BmiI GGNNCC 9 cut(s) 61, 130, 763, 1741, 2134, 2368, 3694, 3695, 3720
BmrFI CCNGG 8 cut(s) 597, 785, 2581, 2600, 2888, 2999, 3304, 3490
BmrI ACTGGG 2 cut(s) 12, 1447
BmsI GCATC 6 cut(s) 1381, 2295, 2383, 2890, 2929, 3103
BmtI GCTAGC 1 cut(s) 557
BmuI ACTGGG 2 cut(s) 12, 1447
BpiI GAAGAC 3 cut(s) 820, 1845, 3000
BplI GAGNNNNNCTC 4 cut(s) 2074, 2106, 2550, 2582
Bpu10I CCTNAGC 3 cut(s) 216, 888, 1956
Bpu14I TTCGAA 1 cut(s) 3502
BpuEI CTTGAG 3 cut(s) 1283, 1446, 3233
BpuMI CCSGG 4 cut(s) 597, 2888, 2999, 3304
Bsa29I ATCGAT 2 cut(s) 1035, 3111
BsaAI YACGTR 2 cut(s) 526, 1099
BsaI GGTCTC 1 cut(s) 3011
BsaWI WCCGGW 6 cut(s) 515, 1076, 1822, 2050, 2643, 3458
BsaXI ACNNNNNCTCC 8 cut(s) 885, 915, 1661, 1691, 1739, 1769, 3592, 3622
Bsc4I CCNNNNNNNGG 9 cut(s) 790, 1253, 2643, 2716, 2787, 2929, 3203, 3204, 3489
Bse118I RCCGGY 3 cut(s) 1736, 2744, 2903
Bse1I ACTGG 5 cut(s) 18, 328, 807, 1453, 1596
Bse3DI GCAATG 2 cut(s) 1530, 2892
BseAI TCCGGA 4 cut(s) 515, 2050, 2643, 3458
BseBI CCWGG 4 cut(s) 785, 2581, 2600, 3490
BseCI ATCGAT 2 cut(s) 1035, 3111
BseGI GGATG 6 cut(s) 351, 1075, 1683, 2310, 2356, 2663
BseLI CCNNNNNNNGG 9 cut(s) 790, 1253, 2643, 2716, 2787, 2929, 3203, 3204, 3489
BseMI GCAATG 2 cut(s) 1530, 2892
BseMII CTCAG 7 cut(s) 230, 432, 902, 1404, 1490, 1970, 2466
BseNI ACTGG 5 cut(s) 18, 328, 807, 1453, 1596
BsePI GCGCGC 1 cut(s) 3540
BseRI GAGGAG 2 cut(s) 1556, 3459
BseSI GKGCMC 2 cut(s) 2081, 3779
BseX3I CGGCCG 1 cut(s) 1645
BseYI CCCAGC 2 cut(s) 1394, 1418
BsgI GTGCAG 2 cut(s) 3470, 3590
Bsh1236I CGCG 8 cut(s) 1667, 1764, 2624, 2960, 3389, 3542, 3578, 3588
Bsh1285I CGRYCG 4 cut(s) 1313, 1648, 3226, 3557
BshNI GGYRCC 1 cut(s) 2366
BshVI ATCGAT 2 cut(s) 1035, 3111
BsiEI CGRYCG 4 cut(s) 1313, 1648, 3226, 3557
BsiHKAI GWGCWC 5 cut(s) 1755, 1969, 2081, 2882, 3666
BsiHKCI CYCGRG 2 cut(s) 1201, 3008
BslFI GGGAC 4 cut(s) 18, 1768, 3319, 3706
BslI CCNNNNNNNGG 9 cut(s) 790, 1253, 2643, 2716, 2787, 2929, 3203, 3204, 3489
BsmAI GTCTC 3 cut(s) 442, 2023, 3011
BsmFI GGGAC 4 cut(s) 18, 1768, 3319, 3706
BsmI GAATGC 1 cut(s) 253
Bso31I GGTCTC 1 cut(s) 3011
BsoBI CYCGRG 2 cut(s) 1201, 3008
Bsp119I TTCGAA 1 cut(s) 3502
Bsp1286I GDGCHC 8 cut(s) 764, 1755, 1969, 2081, 2882, 3015, 3666, 3779
Bsp13I TCCGGA 4 cut(s) 515, 2050, 2643, 3458
Bsp1407I TGTACA 1 cut(s) 2440
Bsp19I CCATGG 3 cut(s) 880, 2127, 2136
BspCNI CTCAG 7 cut(s) 229, 431, 901, 1405, 1489, 1969, 2465
BspDI ATCGAT 2 cut(s) 1035, 3111
BspEI TCCGGA 4 cut(s) 515, 2050, 2643, 3458
BspFNI CGCG 8 cut(s) 1667, 1764, 2624, 2960, 3389, 3542, 3578, 3588
BspHI TCATGA 3 cut(s) 48, 2511, 2865
BspLI GGNNCC 9 cut(s) 61, 130, 763, 1741, 2134, 2368, 3694, 3695, 3720
BspOI GCTAGC 1 cut(s) 557
BspPI GGATC 6 cut(s) 123, 136, 1238, 1532, 2169, 3188
BspQI GCTCTTC 1 cut(s) 759
BspT104I TTCGAA 1 cut(s) 3502
BspT107I GGYRCC 1 cut(s) 2366
BspTNI GGTCTC 1 cut(s) 3011
BsrBI CCGCTC 2 cut(s) 1249, 1489
BsrDI GCAATG 2 cut(s) 1530, 2892
BsrFI RCCGGY 3 cut(s) 1736, 2744, 2903
BsrGI TGTACA 1 cut(s) 2440
BsrI ACTGG 5 cut(s) 18, 328, 807, 1453, 1596
BssAI RCCGGY 3 cut(s) 1736, 2744, 2903
BssHII GCGCGC 1 cut(s) 3540
BssSI CACGAG 3 cut(s) 1536, 2080, 3134
BssT1I CCWWGG 7 cut(s) 133, 880, 2127, 2136, 2826, 2928, 3714
Bst2BI CACGAG 3 cut(s) 1536, 2080, 3134
Bst2UI CCWGG 4 cut(s) 785, 2581, 2600, 3490
Bst6I CTCTTC 5 cut(s) 642, 759, 1015, 1398, 1821
BstAUI TGTACA 1 cut(s) 2440
BstBAI YACGTR 2 cut(s) 526, 1099
BstBI TTCGAA 1 cut(s) 3502
BstDSI CCRYGG 6 cut(s) 880, 1212, 2127, 2136, 2622, 2916
BstENI CCTNNNNNAGG 1 cut(s) 2927
BstF5I GGATG 6 cut(s) 351, 1075, 1683, 2310, 2356, 2663
BstFNI CGCG 8 cut(s) 1667, 1764, 2624, 2960, 3389, 3542, 3578, 3588
BstH2I RGCGCY 2 cut(s) 260, 888
BstMAI GTCTC 3 cut(s) 442, 2023, 3011
BstMCI CGRYCG 4 cut(s) 1313, 1648, 3226, 3557
BstNI CCWGG 4 cut(s) 785, 2581, 2600, 3490
BstNSI RCATGY 4 cut(s) 86, 407, 987, 2578
BstSCI CCNGG 8 cut(s) 595, 783, 2579, 2598, 2886, 2997, 3302, 3488
BstSFI CTRYAG 1 cut(s) 3723
BstSLI GKGCMC 2 cut(s) 2081, 3779
BstUI CGCG 8 cut(s) 1667, 1764, 2624, 2960, 3389, 3542, 3578, 3588
BstV2I GAAGAC 3 cut(s) 820, 1845, 3000
BstX2I RGATCY 2 cut(s) 128, 2408
BstXI CCANNNNNNTGG 2 cut(s) 157, 1591
BstYI RGATCY 2 cut(s) 128, 2408
BstZI CGGCCG 1 cut(s) 1645
Bsu15I ATCGAT 2 cut(s) 1035, 3111
BsuI GTATCC 4 cut(s) 1460, 1571, 2735, 3413
BsuTUI ATCGAT 2 cut(s) 1035, 3111
BtgI CCRYGG 6 cut(s) 880, 1212, 2127, 2136, 2622, 2916
BtgZI GCGATG 3 cut(s) 907, 1648, 3385
BtrI CACGTC 4 cut(s) 1699, 1782, 3429, 3767
BtsCI GGATG 6 cut(s) 351, 1075, 1683, 2310, 2356, 2663
BtsI GCAGTG 2 cut(s) 258, 2637
BtsIMutI CAGTG 4 cut(s) 258, 897, 1589, 2637
CciI TCATGA 3 cut(s) 48, 2511, 2865
Cfr10I RCCGGY 3 cut(s) 1736, 2744, 2903
Cfr42I CCGCGG 1 cut(s) 2625
ClaI ATCGAT 2 cut(s) 1035, 3111
CseI GACGC 1 cut(s) 3584
DraIII CACNNNGTG 1 cut(s) 3432
DrdI GACNNNNNNGTC 1 cut(s) 3700
DseDI GACNNNNNNGTC 1 cut(s) 3700
EaeI YGGCCR 4 cut(s) 804, 1645, 1771, 3711
EagI CGGCCG 1 cut(s) 1645
Eam1104I CTCTTC 5 cut(s) 642, 759, 1015, 1398, 1821
EarI CTCTTC 5 cut(s) 642, 759, 1015, 1398, 1821
EciI GGCGGA 3 cut(s) 948, 1206, 1781
Ecl136II GAGCTC 2 cut(s) 1753, 1967
EclXI CGGCCG 1 cut(s) 1645
Eco130I CCWWGG 7 cut(s) 133, 880, 2127, 2136, 2826, 2928, 3714
Eco24I GRGCYC 4 cut(s) 764, 1755, 1969, 3015
Eco31I GGTCTC 1 cut(s) 3011
Eco47I GGWCC 5 cut(s) 60, 69, 2920, 3693, 3719
Eco52I CGGCCG 1 cut(s) 1645
Eco53kI GAGCTC 2 cut(s) 1753, 1967
Eco57I CTGAAG 4 cut(s) 195, 740, 1277, 3012
Eco72I CACGTG 1 cut(s) 1099
Eco88I CYCGRG 2 cut(s) 1201, 3008
EcoICRI GAGCTC 2 cut(s) 1753, 1967
EcoNI CCTNNNNNAGG 1 cut(s) 2927
EcoO109I RGGNCCY 3 cut(s) 2708, 3693, 3719
EcoRI GAATTC 4 cut(s) 740, 1088, 1103, 2356
EcoRII CCWGG 4 cut(s) 783, 2579, 2598, 3488
EcoT14I CCWWGG 7 cut(s) 133, 880, 2127, 2136, 2826, 2928, 3714
EcoT22I ATGCAT 1 cut(s) 84
EcoT38I GRGCYC 4 cut(s) 764, 1755, 1969, 3015
ErhI CCWWGG 7 cut(s) 133, 880, 2127, 2136, 2826, 2928, 3714
FaqI GGGAC 4 cut(s) 18, 1768, 3319, 3706
FauI CCCGC 5 cut(s) 1159, 1482, 3073, 3109, 3428
FbaI TGATCA 6 cut(s) 168, 426, 1972, 2235, 2344, 2484
FblI GTMKAC 1 cut(s) 3348
FokI GGATG 6 cut(s) 358, 1082, 1670, 2317, 2363, 2650
FriOI GRGCYC 4 cut(s) 764, 1755, 1969, 3015
GsaI CCCAGC 2 cut(s) 1398, 1422
HaeII RGCGCY 2 cut(s) 260, 888
HgaI GACGC 1 cut(s) 3584
HincII GTYRAC 1 cut(s) 3349
HindII GTYRAC 1 cut(s) 3349
HindIII AAGCTT 2 cut(s) 1289, 2816
HphI GGTGA 8 cut(s) 149, 195, 1091, 1228, 1339, 2416, 2432, 2887
Hpy166II GTNNAC 5 cut(s) 203, 1212, 2079, 3328, 3349
Hpy8I GTNNAC 5 cut(s) 203, 1212, 2079, 3328, 3349
HpyCH4IV ACGT 8 cut(s) 525, 1098, 1698, 1781, 2835, 3293, 3428, 3766
HpySE526I ACGT 8 cut(s) 525, 1098, 1698, 1781, 2835, 3293, 3428, 3766
KflI GGGWCCC 1 cut(s) 3693
Kpn2I TCCGGA 4 cut(s) 515, 2050, 2643, 3458
KroI GCCGGC 1 cut(s) 2744
KroNI GCCGGC 1 cut(s) 2746
Ksp22I TGATCA 6 cut(s) 168, 426, 1972, 2235, 2344, 2484
KspI CCGCGG 1 cut(s) 2625
LguI GCTCTTC 1 cut(s) 759
LmnI GCTCC 3 cut(s) 767, 1745, 1972
LweI GCATC 6 cut(s) 1381, 2295, 2383, 2890, 2929, 3103
MaeII ACGT 8 cut(s) 525, 1098, 1698, 1781, 2835, 3293, 3428, 3766
MaeIII GTNAC 5 cut(s) 406, 2458, 2650, 2782, 3029
MbiI CCGCTC 2 cut(s) 1249, 1489
MfeI CAATTG 1 cut(s) 2505
MflI RGATCY 2 cut(s) 128, 2408
MhlI GDGCHC 8 cut(s) 764, 1755, 1969, 2081, 2882, 3015, 3666, 3779
MlsI TGGCCA 3 cut(s) 806, 1773, 3713
MluNI TGGCCA 3 cut(s) 806, 1773, 3713
MlyI GAGTC 6 cut(s) 1589, 1936, 2083, 2376, 2568, 2748
MmeI TCCRAC 7 cut(s) 697, 1065, 1822, 2661, 2752, 3336, 3504
Mox20I TGGCCA 3 cut(s) 806, 1773, 3713
Mph1103I ATGCAT 1 cut(s) 84
MroI TCCGGA 4 cut(s) 515, 2050, 2643, 3458
MroNI GCCGGC 1 cut(s) 2744
MscI TGGCCA 3 cut(s) 806, 1773, 3713
MseI TTAA 6 cut(s) 602, 1362, 2175, 3201, 3410, 3781
MslI CAYNNNNRTG 4 cut(s) 77, 87, 286, 1842
Msp20I TGGCCA 3 cut(s) 806, 1773, 3713
MspA1I CMGCKG 4 cut(s) 1234, 2113, 2624, 3475
MspR9I CCNGG 8 cut(s) 597, 785, 2581, 2600, 2888, 2999, 3304, 3490
MunI CAATTG 1 cut(s) 2505
Mva1269I GAATGC 1 cut(s) 253
MvaI CCWGG 4 cut(s) 785, 2581, 2600, 3490
MvnI CGCG 8 cut(s) 1667, 1764, 2624, 2960, 3389, 3542, 3578, 3588
NaeI GCCGGC 1 cut(s) 2746
NciI CCSGG 4 cut(s) 597, 2888, 2999, 3304
NcoI CCATGG 3 cut(s) 880, 2127, 2136
NgoMIV GCCGGC 1 cut(s) 2744
NheI GCTAGC 1 cut(s) 553
NlaIV GGNNCC 9 cut(s) 61, 130, 763, 1741, 2134, 2368, 3694, 3695, 3720
NmeAIII GCCGAG 1 cut(s) 1021
NmuCI GTSAC 4 cut(s) 406, 2650, 2782, 3029
NsiI ATGCAT 1 cut(s) 84
NspI RCATGY 4 cut(s) 86, 407, 987, 2578
NspV TTCGAA 1 cut(s) 3502
PaeI GCATGC 1 cut(s) 86
PaeR7I CTCGAG 1 cut(s) 3008
PagI TCATGA 3 cut(s) 48, 2511, 2865
PauI GCGCGC 1 cut(s) 3540
PciI ACATGT 1 cut(s) 403
PciSI GCTCTTC 1 cut(s) 759
PcsI WCGNNNNNNNCGW 2 cut(s) 1704, 1940
PctI GAATGC 1 cut(s) 253
PdiI GCCGGC 1 cut(s) 2746
PfeI GAWTC 4 cut(s) 457, 1037, 1261, 1601
PflMI CCANNNNNTGG 1 cut(s) 790
PfoI TCCNGGA 1 cut(s) 2579
Ple19I CGATCG 2 cut(s) 1313, 3557
PleI GAGTC 6 cut(s) 1589, 1935, 2083, 2376, 2567, 2748
PmaCI CACGTG 1 cut(s) 1099
PmlI CACGTG 1 cut(s) 1099
PpsI GAGTC 6 cut(s) 1589, 1935, 2083, 2376, 2567, 2748
Ppu21I YACGTR 2 cut(s) 526, 1099
PpuMI RGGWCCY 2 cut(s) 3693, 3719
PscI ACATGT 1 cut(s) 403
PsiI TTATAA 1 cut(s) 539
Psp124BI GAGCTC 2 cut(s) 1755, 1969
Psp1406I AACGTT 1 cut(s) 3293
Psp5II RGGWCCY 2 cut(s) 3693, 3719
Psp6I CCWGG 4 cut(s) 783, 2579, 2598, 3488
PspCI CACGTG 1 cut(s) 1099
PspFI CCCAGC 2 cut(s) 1394, 1418
PspGI CCWGG 4 cut(s) 783, 2579, 2598, 3488
PspN4I GGNNCC 9 cut(s) 61, 130, 763, 1741, 2134, 2368, 3694, 3695, 3720
PspPPI RGGWCCY 2 cut(s) 3693, 3719
PsuI RGATCY 2 cut(s) 128, 2408
PteI GCGCGC 1 cut(s) 3540
PvuI CGATCG 2 cut(s) 1313, 3557
RgaI GCGATCGC 1 cut(s) 1313
RseI CAYNNNNRTG 4 cut(s) 77, 87, 286, 1842
SacI GAGCTC 2 cut(s) 1755, 1969
SacII CCGCGG 1 cut(s) 2625
SalI GTCGAC 1 cut(s) 3347
SapI GCTCTTC 1 cut(s) 759
SaqAI TTAA 6 cut(s) 602, 1362, 2175, 3201, 3410, 3781
ScaI AGTACT 2 cut(s) 971, 2279
SchI GAGTC 6 cut(s) 1589, 1936, 2083, 2376, 2568, 2748
ScrFI CCNGG 8 cut(s) 597, 785, 2581, 2600, 2888, 2999, 3304, 3490
SduI GDGCHC 8 cut(s) 764, 1755, 1969, 2081, 2882, 3015, 3666, 3779
SfaAI GCGATCGC 1 cut(s) 1313
SfaNI GCATC 6 cut(s) 1381, 2295, 2383, 2890, 2929, 3103
SfcI CTRYAG 1 cut(s) 3723
Sfr274I CTCGAG 1 cut(s) 3008
Sfr303I CCGCGG 1 cut(s) 2625
SfuI TTCGAA 1 cut(s) 3502
SgfI GCGATCGC 1 cut(s) 1313
SgrBI CCGCGG 1 cut(s) 2625
SinI GGWCC 5 cut(s) 60, 69, 2920, 3693, 3719
SlaI CTCGAG 1 cut(s) 3008
SmiMI CAYNNNNRTG 4 cut(s) 77, 87, 286, 1842
SmlI CTYRAG 4 cut(s) 1298, 1461, 3008, 3248
SmoI CTYRAG 4 cut(s) 1298, 1461, 3008, 3248
SpeI ACTAGT 1 cut(s) 3605
SphI GCATGC 1 cut(s) 86
SspI AATATT 1 cut(s) 193
SstI GAGCTC 2 cut(s) 1755, 1969
StyD4I CCNGG 8 cut(s) 595, 783, 2579, 2598, 2886, 2997, 3302, 3488
StyI CCWWGG 7 cut(s) 133, 880, 2127, 2136, 2826, 2928, 3714
TaiI ACGT 8 cut(s) 528, 1101, 1701, 1784, 2838, 3296, 3431, 3769
TaqII GACCGA 2 cut(s) 2688, 2985
TatI WGTACW 6 cut(s) 264, 651, 969, 2104, 2277, 2440
TauI GCSGC 5 cut(s) 533, 752, 1200, 1650, 2624
TfiI GAWTC 4 cut(s) 457, 1037, 1261, 1601
Tru1I TTAA 6 cut(s) 602, 1362, 2175, 3201, 3410, 3781
Tru9I TTAA 6 cut(s) 602, 1362, 2175, 3201, 3410, 3781
TscAI CASTG 4 cut(s) 258, 904, 1596, 2637
TseFI GTSAC 4 cut(s) 406, 2650, 2782, 3029
Tsp45I GTSAC 4 cut(s) 406, 2650, 2782, 3029
TspDTI ATGAA 9 cut(s) 65, 67, 200, 512, 835, 1435, 2010, 2854, 3388
TspGWI ACGGA 5 cut(s) 624, 754, 2174, 2823, 2933
TspRI CASTG 4 cut(s) 258, 904, 1596, 2637
Van91I CCANNNNNTGG 1 cut(s) 790
VneI GTGCAC 1 cut(s) 2077
VpaK11BI GGWCC 5 cut(s) 60, 69, 2920, 3693, 3719
XagI CCTNNNNNAGG 1 cut(s) 2927
XapI RAATTY 5 cut(s) 499, 740, 1088, 1103, 2356
XbaI TCTAGA 1 cut(s) 2378
XceI RCATGY 4 cut(s) 86, 407, 987, 2578
XcmI CCANNNNNNNNNTGG 3 cut(s) 1588, 1766, 2134
XhoI CTCGAG 1 cut(s) 3008
XmiI GTMKAC 1 cut(s) 3348
ZrmI AGTACT 2 cut(s) 971, 2279
Zsp2I ATGCAT 1 cut(s) 84
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.