pycom16g08980

Multidrug resistance

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr16
OG0000317
Physical Location & Seq
Reverse (-)
6004142 .. 6008738
4597 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom16g08980.2

Sequence Viewer

Length: 3807 bp
ATGGGGAAGAAAGGTGGAATCTTTAGGTATGCAGATGGTGTCGATAAGTTGCTAATGGTGTTCGGGACTCTGGGTAGCATCGGAGATGGGTTGATGACACCGCTGACTATGTTCGTTCTCAGCCGTGTGATCAATGAGTATGGAGGTGCTACTCTTTCTTTCTCCAATGAAGTTGTGGACAAGTACGGACTCAGGCTGCTAATTCTTGCAATTGGGGTTGGAATATCTGCTTTCATGGAAGGAGTTTGTTGGACTAGAACTGCTGAGAGGCAGGCATCTCGGATGAGAATTGAGTACTTAAAATCAGTCCTCAGGCAAGAAGTTGCCTTCTTTGACAGTCAGGCCAATTCTTCCATTACCTTCCAAGTCATTTCTACTATCTCTTCCGATGCTCATTTAATCCAAGACACAATCGCGGAAAAGATACCGAATTGCTTGGCACATCTCTCGTCATTTATCTTCTGCTTTCCTGTTTCCTTTGTGCTTTCTTGGCGACTGGCACTGGCTGCTCTTCCATGCTCGATCATGTTTATGATCCCAGGAATTGGAATTGGGAATGTAATGAAGAAATTGGGAGGTAAGATAGCTGGTGCCTATGGGGTTGCTGGAGGGATTGCGGAACAAGCAATCTCATCAATCCGTACAGTTTATTCGTACGTTGGGGAGAATCAAACACTAAAAAAGTTCAGCATTGCTCTCGAAAAATGCACAGCGCTAGGCATAAAGCAAGGCTTCACAAAGGGATTGCTGATAGGCAGCATGGGAATGATTTATGCTGCTTGGGCTTTTCAGGCTTGGGTTGGTAGCAAACTTGTTACCGAGAGAGGAGAAAAAGGTGGCCTTGTTTTCATTTCTGGAATCTGCGTAATTCTCGGAGGAATCGCCATAATGAATGCGCTTCCAATTCTCGCTGCCATCACCGCAGCTACTGCTGCTGCTACTCGAATATCTGAAATGATTGATCGAATTCCCGCTATTGATTCTGAAGATGAAAGGGGAAAAGTTTTAACATTTGTGAAAGGAGACATCGAATTTAGAAAGGTTGACTTCTGCTATCCTTCAAGACCTGATACCCTGGTTCTTCAAGGACTAAATCTCAAGGTCCAAGCTGGTAAGATGATAGGTCTTGTTGGAGGCAGTGGTTCGGGCAAGTCAACAATCATTGCTTTGCTCGAAAGATTTTATGATCCAGTAAAAGGAGAGGTTCTCCTCGACGGATACAAGTTAAAGAAGTTTCAGCTACAGTGGTTGAGATCACAGATGGGACTGGTCAATCAAGAGCCTATTCTCTTTGCAACATCGATAAAAGAGAATATTTTGTTCGGAAAGGAAGGAGCTTCAATGGAAAATGTAATAAGTGCAGCTAAGGATGCAAATGCACATGACTTCATTTCTAAGCTACCTCAAGGATATGATACTCAAGTTGGGCAATTCGGAGTTCAATTGTCTGGAGGACAAAAGCAGAGGATTTCAATAGCGAGAGCAATCATCAGAGACCCGAAAATTCTTTTGCTTGATGAAGCCACAAGTGCTTTGGATGCTCAATCCGAAAAAGTTGTACAGGAAGCCTTGGACCATGCTTCACAAGGAAGAACAACAATCGTCATAGCTCACCGCCTCACCACAATTCGCAAGGCTGATCTGATAGTGGTTCTTCAATCGGGTAGAGTGGTGGAAAAGGGAACTCATGAGGACTTAGTCCAGAAGAACTATGGAGAAGGTGGAGCTTACAGTAAGATGCTGCAATTACAGCAACAGGCAATGCAGAACGGTGTCCCTGAGATATCCTATTATCCGGACGACATTATAGACCAAAAAACCAAACATCTGCGAAATCCGCAGACACCAAAAAGTGGGAGATCAAGCTGGAAAAACAGCCCTGCGCCAAGGAGTGGGGAAACAAACTGGCACACACCAAGGAGTGTGACACCAAGCTGGCAAAACAGCCCGGCTTATCCAGTCACCCCAATATTTTCCATCAGCATCGAGAACTCTTTCCAAGCACACTACGATGACTTTGACGATGAAAACATCTCTCATCGTTCCTCCTCCCTCTGGCGTTTATTGCAAATGAATGCACCTGAGTGGAAGCAAGCAATACTAGGGTGTATAGGAGCTGCTGGCTTCGGAGCTATCCAGCCAGTTCATGCCTATTGCTTAGGAACAGTCATATCGGTTTATTTCCAAACAGACAAATCCAAGATACTATCAGACACCAGATTCTACTGCTACATCTTCTTAAGCATAGCAGTTCTCAGCTTCATTGCTAATCTCCTCCAACATTACAACTTTGCAGTTATGGGAGAGCGATTATCCAAAAGGGTTCGGATACAAATGCTCGAAAAAATTCTCACCTTTGAGATAGGGTGGTTTGATAAAGATGAAAACACAAGTGCTGCCATCTGTGCACGGCTAACCACTGAAGCAAGCATGGTTAGATCCCTAACCGCAGATCGTATATCAATGTTGGTTCAGGTTTTCTTCAGTGCCTCGATAGCTTTTGTGATTGGACTCATGATCACATGGAGGATAGCCATTGTAATGATTGCGATACAGCCCTTGCTCATAGGAAGCTTTTATTCGAGGAGTGTTCTAATGAAGAGTATGTCTATAAAAGCACAGAAAGCGCAAGCAGAGGGCAGTCAACTAGCTAGTGAAGCTACTTTCAACCATAGGACTATCACTGCATTTTCTTCTCAGGATAGAATACTAAAGCTATTTGGAGATGCAATGCGAGGGCCGCGGAAGGAGAACATCAAACAGTCATGGATTTCAGGTTTGGGTCTGTTCAGCTCCCAATTTTTGACAACAGCTGCAATAGCCTTGACTTATTGGTATGGAGGAAGGCTAATGAATCGGAAGCTAGTGCCGGCGAAACACCTATTTCAAGTCTTTTTCATCTTAATGAGCACCGGTAAGAACATTGCAGACGCGGGAAGCATGACTACCGATCTAGCCAGAGGGGGAAAAGCCATCAAGTCGATATTTTCAATCCTAGACAGAGACAGTGAAATTTCTTCTGAAGAGTCTGAAGGGATAAAAAAGATTTTCAAGGGTCACATAGAACTAAAAAATGTAGTATTCGCTTACCCGGTGAGGCCGGACCAGATGATCTTCAAGGGGCTGAATCTGAAAATTGAAGCTGGAAACACAATAGCACTAGTGGGACAGAGTGGTTCTGGAAAATCCACTGTCATTGGCTTGATCGAAAGGTTTTATGACCCGCTAAATGGATCAGTGTTGATAGATGGATGCGACATCAAGCTCTATAATTTGAGGAAATTGAGGTCACAAATTGCTTTGGTAAGCCAGGAGCCTACTCTTTTCGGAGGAACCATCCACGAAAACATTGTGTATGGTAAAGAAAATGCTACGGTTTCTGAAGTAAGGAAGGCTGCAAAACTTGCCAATGCTCATGAATTTATAAGTTCTATGGAAGAGGGGTACGAGACTTACTGTGGCGAAAGAGGAGTGCAGCTATCCGGAGGCCAGAAGCAGAGAATAGCACTTGCTCGAGCAATCCTAAAGAACCCAACAATCCTTCTGTTGGATGAAGCAACCAGCGCTCTTGACAGCGTGTCGGAAAATTTAGTCCAAGCAGCATTGGAGAAGATGATGGTCGGTAGAACATGCGTAGTTGTAGCTCACAGGTTGTCGACAATTCAGAAAGCAGACTCCATAGCTGTGATAGTGAATGGGAAGGTTGCTGAAAAAGGATCACACTCTGAGCTGCTTGCCATTGGAGGTGCCTACCATTCTCTGATAAAACTACAAAGCAGTCACCCTCCACACCAACATAACATATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0016020 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
ko02010 ko04976 ko05206 ko05226 map02010 map04976 map05206 map05226
Pfam Domains
Protein Families
ABC_membrane ABC_tran

Protein Analysis

1269

Amino Acids

138.75

Weight (kDa)

9.18

Isoelectric Point (pI)

34.9

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000317)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G28345 AT3G28360 AT3G28380 AT3G28390 AT3G28415 AT3G28415
fragaria_vesca FvH4_1g06060 FvH4_3g21880 FvH4_4g25260 FvH4_6g49750 FvH4_6g49750 FvH4_6g49750 FvH4_6g49750 FvH4_6g49760
malus_domestica MD00G1221200.v1.1 MD02G1065200.v1.1 MD03G1211200.v1.1 MD09G1041500.v1.1 MD09G1041600.v1.1 MD09G1041900.v1.1 MD15G1196000.v1.1 MD16G1105600.v1.1 MD17G1042800.v1.1
prunus_persica Prupe.1G241000_v2.0.a1 Prupe.3G275900_v2.0.a1 Prupe.3G276100_v2.0.a1 Prupe.3G276100_v2.0.a1 Prupe.4G204300_v2.0.a1 Prupe.7G220300_v2.0.a1
pyrus_communis pycom111g03260 pycom16g08980 pycom17g03840
rosa_chinensis RchiOBHm_Chr2g0091821 RchiOBHm_Chr2g0169781 RchiOBHm_Chr2g0169791 RchiOBHm_Chr2g0169801 RchiOBHm_Chr2g0169841 RchiOBHm_Chr2g0169851 RchiOBHm_Chr2g0169861 RchiOBHm_Chr4g0432501 RchiOBHm_Chr5g0037681 RchiOBHm_Chr5g0037691
rosa_laevigata RLG00000006836 RLG00000016254 RLG00000021903 RLG00000021904 RLG00000021905 RLG00000021906 RLG00000021907 RLG00000021908 RLG00000033801
rosa_multiflora Rmu_sc0001084.1_g000003 Rmu_sc0021402.1_g000003 Rmu_ssc0000389.1_g000016 Rmu_ssc0000389.1_g000019 Rmu_ssc0000389.1_g000024 Rmu_ssc0000389.1_g000027
rosa_roxburghii Rroxscaffold_1G00043250 Rroxscaffold_2G00081770 Rroxscaffold_2G00081780 Rroxscaffold_2G00081790 Rroxscaffold_2G00149710 Rroxscaffold_5G00373960
rosa_rugosa Rorug02G0021300 Rorug02G0543400.1 Rorug02G0543500.1 Rorug02G0543600.1 Rorug02G0543700.1 Rorug02G0543800 Rorug02G0543900 Rorug02G0544000 Rorug04G0259900.1 Rorug05G0166300 Rorug05G0166300
rosa_samantha Rh2AG065900 Rh2AG615300 Rh2AG615400 Rh2AG615500 Rh2AG615600 Rh2AG615700 Rh2AG615800 Rh2AG616000 Rh2AG616200 Rh2BG627600 Rh2BG627700 Rh2BG627800 Rh2BG627900 Rh2BG628000 Rh2BG628100 Rh2CG596900 Rh2CG597000 Rh2CG597100 Rh2CG597200 Rh2DG065000 Rh2DG638400 Rh2DG638600 Rh2DG638700 Rh2DG638800 Rh2DG638900 Rh2DG639000 Rh4AG314700 Rh4BG322400 Rh4CG337600 Rh4DG318300 Rh5AG255800 Rh5BG257900 Rh5CG291300
rosa_wichuraiana Rw0G002540 Rw2G005480 Rw2G051010 Rw2G051020 Rw2G051030 Rw2G051040 Rw4G027360 Rw5G023710 Rw5G024000

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 3425
AccB1I GGYRCC 2 cut(s) 590, 3746
AccB7I CCANNNNNTGG 3 cut(s) 545, 1853, 1892
AccI GTMKAC 1 cut(s) 3656
AccII CGCG 3 cut(s) 416, 2743, 2933
AccIII TCCGGA 2 cut(s) 1795, 3482
AclWI GGATC 5 cut(s) 529, 1181, 2433, 3241, 3724
AcsI RAATTY 7 cut(s) 966, 1031, 1503, 2346, 3012, 3419, 3586
AcuI CTGAAG 6 cut(s) 1005, 2442, 2467, 3042, 3051, 3402
AfaI GTAC 6 cut(s) 185, 296, 643, 656, 1560, 3446
AfeI AGCGCT 2 cut(s) 714, 3565
AfiI CCNNNNNNNGG 7 cut(s) 545, 1196, 1853, 1892, 2055, 3230, 3547
AflII CTTAAG 1 cut(s) 2239
AgeI ACCGGT 1 cut(s) 2912
AhdI GACNNNNNGTC 1 cut(s) 3577
AhlI ACTAGT 1 cut(s) 3160
AjnI CCWGG 3 cut(s) 538, 1074, 3309
AleI CACNNNNGTG 1 cut(s) 2083
Alw21I GWGCWC 2 cut(s) 2410, 2912
Alw26I GTCTC 4 cut(s) 1017, 1488, 2997, 3443
Alw44I GTGCAC 1 cut(s) 2406
AlwI GGATC 5 cut(s) 529, 1181, 2433, 3241, 3724
AlwNI CAGNNNCTG 1 cut(s) 929
Ama87I CYCGRG 1 cut(s) 3513
Aor13HI TCCGGA 2 cut(s) 1795, 3482
Aor51HI AGCGCT 2 cut(s) 714, 3565
AoxI GGCC 5 cut(s) 342, 838, 2738, 3098, 3487
ApaLI GTGCAC 1 cut(s) 2406
ApoI RAATTY 7 cut(s) 966, 1031, 1503, 2346, 3012, 3419, 3586
AsiGI ACCGGT 1 cut(s) 2912
Asp700I GAANNNNTTC 2 cut(s) 1994, 2346
AspLEI GCGC 5 cut(s) 715, 898, 1885, 2629, 3566
AspS9I GGNCC 4 cut(s) 1102, 1573, 2738, 3103
AsuC2I CCSGG 2 cut(s) 1949, 3092
AsuHPI GGTGA 7 cut(s) 910, 1604, 1612, 1954, 2344, 3106, 3773
AvaI CYCGRG 1 cut(s) 3513
AvaII GGWCC 3 cut(s) 1102, 1573, 3103
AxyI CCTNAGG 1 cut(s) 311
BaeGI GKGCMC 1 cut(s) 2410
BaeI ACNNNNGTAYC 4 cut(s) 1062, 1095, 1407, 1440
BanI GGYRCC 2 cut(s) 590, 3746
BarI GAAGNNNNNNTAC 2 cut(s) 2929, 2961
Bbv12I GWGCWC 2 cut(s) 2410, 2912
BceAI ACGGC 2 cut(s) 108, 2426
BcgI CGANNNNNNTGC 6 cut(s) 260, 294, 429, 463, 679, 713
BciT130I CCWGG 3 cut(s) 540, 1076, 3311
BciVI GTATCC 2 cut(s) 1211, 2322
BclI TGATCA 2 cut(s) 129, 2517
BcnI CCSGG 2 cut(s) 1949, 3092
BcoDI GTCTC 4 cut(s) 1017, 1488, 2997, 3443
BcuI ACTAGT 1 cut(s) 3160
BfaI CTAG 9 cut(s) 255, 716, 2102, 2648, 2652, 2864, 2954, 2996, 3161
BfmI CTRYAG 1 cut(s) 1241
BfoI RGCGCY 2 cut(s) 716, 3567
BfrI CTTAAG 1 cut(s) 2239
BfuI GTATCC 2 cut(s) 1211, 2322
BmcAI AGTACT 1 cut(s) 296
Bme1390I CCNGG 5 cut(s) 540, 1076, 1949, 3092, 3311
Bme18I GGWCC 3 cut(s) 1102, 1573, 3103
BmeRI GACNNNNNGTC 1 cut(s) 3577
BmeT110I CYCGRG 1 cut(s) 3513
BmgT120I GGNCC 4 cut(s) 1102, 1573, 2738, 3103
BmiI GGNNCC 4 cut(s) 592, 3315, 3334, 3748
BmrFI CCNGG 5 cut(s) 540, 1076, 1949, 3092, 3311
BmsI GCATC 9 cut(s) 87, 284, 379, 1360, 1528, 1728, 1992, 2716, 3242
BplI GAGNNNNNCTC 2 cut(s) 1191, 1223
BpmI CTGGAG 2 cut(s) 627, 1470
Bpu10I CCTNAGC 2 cut(s) 1365, 2158
BpuEI CTTGAG 3 cut(s) 1082, 1389, 1404
BpuMI CCSGG 2 cut(s) 1949, 3092
Bsa29I ATCGAT 1 cut(s) 1301
BsaI GGTCTC 1 cut(s) 1488
BsaJI CCNNGG 6 cut(s) 538, 1074, 1569, 1886, 1916, 2741
BsaWI WCCGGW 3 cut(s) 1795, 2912, 3482
Bsc4I CCNNNNNNNGG 7 cut(s) 545, 1196, 1853, 1892, 2055, 3230, 3547
Bse118I RCCGGY 2 cut(s) 2869, 2912
Bse1I ACTGG 7 cut(s) 501, 507, 1190, 1272, 1910, 1958, 2141
Bse21I CCTNAGG 1 cut(s) 311
Bse3DI GCAATG 6 cut(s) 690, 1161, 1767, 2262, 2736, 2922
BseAI TCCGGA 2 cut(s) 1795, 3482
BseBI CCWGG 3 cut(s) 540, 1076, 3311
BseCI ATCGAT 1 cut(s) 1301
BseDI CCNNGG 6 cut(s) 538, 1074, 1569, 1886, 1916, 2741
BseGI GGATG 6 cut(s) 288, 1375, 1543, 3257, 3336, 3556
BseLI CCNNNNNNNGG 7 cut(s) 545, 1196, 1853, 1892, 2055, 3230, 3547
BseMI GCAATG 6 cut(s) 690, 1161, 1767, 2262, 2736, 2922
BseMII CTCAG 9 cut(s) 133, 205, 255, 325, 1770, 2073, 2269, 2711, 3717
BseNI ACTGG 7 cut(s) 501, 507, 1190, 1272, 1910, 1958, 2141
BseRI GAGGAG 6 cut(s) 840, 1199, 2038, 2264, 2599, 3483
BseSI GKGCMC 1 cut(s) 2410
BsgI GTGCAG 2 cut(s) 1380, 3494
Bsh1236I CGCG 3 cut(s) 416, 2743, 2933
BshFI GGCC 5 cut(s) 344, 840, 2740, 3100, 3489
BshNI GGYRCC 2 cut(s) 590, 3746
BshTI ACCGGT 1 cut(s) 2912
BshVI ATCGAT 1 cut(s) 1301
BsiHKAI GWGCWC 2 cut(s) 2410, 2912
BsiHKCI CYCGRG 1 cut(s) 3513
BsiSI CCGG 7 cut(s) 1796, 1949, 2870, 2913, 3092, 3101, 3483
BsiWI CGTACG 1 cut(s) 654
BslFI GGGAC 4 cut(s) 79, 1278, 1760, 3180
BslI CCNNNNNNNGG 7 cut(s) 545, 1196, 1853, 1892, 2055, 3230, 3547
BsmAI GTCTC 4 cut(s) 1017, 1488, 2997, 3443
BsmFI GGGAC 4 cut(s) 79, 1278, 1760, 3180
BsmI GAATGC 2 cut(s) 898, 2080
BsnI GGCC 5 cut(s) 344, 840, 2740, 3100, 3489
Bso31I GGTCTC 1 cut(s) 1488
BsoBI CYCGRG 1 cut(s) 3513
Bsp1286I GDGCHC 2 cut(s) 2410, 2912
Bsp13I TCCGGA 2 cut(s) 1795, 3482
Bsp1407I TGTACA 1 cut(s) 1558
BspANI GGCC 5 cut(s) 344, 840, 2740, 3100, 3489
BspCNI CTCAG 9 cut(s) 132, 204, 256, 324, 1771, 2074, 2268, 2710, 3718
BspDI ATCGAT 1 cut(s) 1301
BspEI TCCGGA 2 cut(s) 1795, 3482
BspFNI CGCG 3 cut(s) 416, 2743, 2933
BspHI TCATGA 3 cut(s) 1687, 2514, 3415
BspLI GGNNCC 4 cut(s) 592, 3315, 3334, 3748
BspPI GGATC 5 cut(s) 529, 1181, 2433, 3241, 3724
BspQI GCTCTTC 1 cut(s) 516
BspT107I GGYRCC 2 cut(s) 590, 3746
BspTI CTTAAG 1 cut(s) 2239
BspTNI GGTCTC 1 cut(s) 1488
BsrDI GCAATG 6 cut(s) 690, 1161, 1767, 2262, 2736, 2922
BsrFI RCCGGY 2 cut(s) 2869, 2912
BsrGI TGTACA 1 cut(s) 1558
BsrI ACTGG 7 cut(s) 501, 507, 1190, 1272, 1910, 1958, 2141
BssAI RCCGGY 2 cut(s) 2869, 2912
BssECI CCNNGG 6 cut(s) 538, 1074, 1569, 1886, 1916, 2741
BssT1I CCWWGG 3 cut(s) 1569, 1886, 1916
Bst2UI CCWGG 3 cut(s) 540, 1076, 3311
Bst6I CTCTTC 5 cut(s) 388, 516, 2594, 3018, 3432
BstAFI CTTAAG 1 cut(s) 2239
BstAPI GCANNNNNTGC 3 cut(s) 506, 929, 3404
BstAUI TGTACA 1 cut(s) 1558
BstC8I GCNNGC 8 cut(s) 273, 1937, 2094, 2122, 2428, 2631, 2871, 3735
BstDSI CCRYGG 1 cut(s) 2741
BstF5I GGATG 6 cut(s) 288, 1375, 1543, 3257, 3336, 3556
BstFNI CGCG 3 cut(s) 416, 2743, 2933
BstH2I RGCGCY 2 cut(s) 716, 3567
BstHHI GCGC 5 cut(s) 715, 898, 1885, 2629, 3566
BstMAI GTCTC 4 cut(s) 1017, 1488, 2997, 3443
BstNI CCWGG 3 cut(s) 540, 1076, 3311
BstNSI RCATGY 1 cut(s) 3633
BstSCI CCNGG 5 cut(s) 538, 1074, 1947, 3090, 3309
BstSFI CTRYAG 1 cut(s) 1241
BstSLI GKGCMC 1 cut(s) 2410
BstUI CGCG 3 cut(s) 416, 2743, 2933
BstX2I RGATCY 1 cut(s) 2438
BstYI RGATCY 1 cut(s) 2438
Bsu15I ATCGAT 1 cut(s) 1301
Bsu36I CCTNAGG 1 cut(s) 311
BsuI GTATCC 2 cut(s) 1211, 2322
BsuRI GGCC 5 cut(s) 344, 840, 2740, 3100, 3489
BsuTUI ATCGAT 1 cut(s) 1301
BtgI CCRYGG 1 cut(s) 2741
BtsCI GGATG 6 cut(s) 288, 1375, 1543, 3257, 3336, 3556
BtsI GCAGTG 2 cut(s) 1144, 2682
BtsIMutI CAGTG 9 cut(s) 500, 1144, 1250, 2418, 2491, 2682, 3013, 3189, 3243
Cac8I GCNNGC 8 cut(s) 273, 1937, 2094, 2122, 2428, 2631, 2871, 3735
CaiI CAGNNNCTG 1 cut(s) 929
CciI TCATGA 3 cut(s) 1687, 2514, 3415
CfoI GCGC 5 cut(s) 715, 898, 1885, 2629, 3566
Cfr10I RCCGGY 2 cut(s) 2869, 2912
Cfr13I GGNCC 4 cut(s) 1102, 1573, 2738, 3103
Cfr42I CCGCGG 1 cut(s) 2744
ClaI ATCGAT 1 cut(s) 1301
CseI GACGC 1 cut(s) 2939
Csp6I GTAC 6 cut(s) 184, 295, 642, 655, 1559, 3445
CspAI ACCGGT 1 cut(s) 2912
CspCI CAANNNNNGTGG 2 cut(s) 3178, 3213
CviQI GTAC 6 cut(s) 184, 295, 642, 655, 1559, 3445
DriI GACNNNNNGTC 1 cut(s) 3577
Eam1104I CTCTTC 5 cut(s) 388, 516, 2594, 3018, 3432
Eam1105I GACNNNNNGTC 1 cut(s) 3577
EarI CTCTTC 5 cut(s) 388, 516, 2594, 3018, 3432
Eco130I CCWWGG 3 cut(s) 1569, 1886, 1916
Eco31I GGTCTC 1 cut(s) 1488
Eco32I GATATC 1 cut(s) 1785
Eco47I GGWCC 3 cut(s) 1102, 1573, 3103
Eco47III AGCGCT 2 cut(s) 714, 3565
Eco57I CTGAAG 6 cut(s) 1005, 2442, 2467, 3042, 3051, 3402
Eco81I CCTNAGG 1 cut(s) 311
Eco88I CYCGRG 1 cut(s) 3513
EcoRI GAATTC 1 cut(s) 966
EcoRII CCWGG 3 cut(s) 538, 1074, 3309
EcoRV GATATC 1 cut(s) 1785
EcoT14I CCWWGG 3 cut(s) 1569, 1886, 1916
ErhI CCWWGG 3 cut(s) 1569, 1886, 1916
FalI AAGNNNNNCTT 6 cut(s) 716, 748, 825, 857, 1031, 1063
FaqI GGGAC 4 cut(s) 79, 1278, 1760, 3180
FauI CCCGC 3 cut(s) 979, 2926, 3231
FauNDI CATATG 1 cut(s) 3803
FbaI TGATCA 2 cut(s) 129, 2517
FblI GTMKAC 1 cut(s) 3656
FokI GGATG 6 cut(s) 295, 1382, 1550, 3264, 3323, 3563
FspBI CTAG 9 cut(s) 255, 716, 2102, 2648, 2652, 2864, 2954, 2996, 3161
GlaI GCGC 5 cut(s) 714, 897, 1884, 2628, 3565
GsuI CTGGAG 2 cut(s) 627, 1470
HaeII RGCGCY 2 cut(s) 716, 3567
HaeIII GGCC 5 cut(s) 344, 840, 2740, 3100, 3489
HapII CCGG 7 cut(s) 1796, 1949, 2870, 2913, 3092, 3101, 3483
HgaI GACGC 1 cut(s) 2939
HhaI GCGC 5 cut(s) 715, 898, 1885, 2629, 3566
Hin6I GCGC 5 cut(s) 713, 896, 1883, 2627, 3564
HinP1I GCGC 5 cut(s) 713, 896, 1883, 2627, 3564
HincII GTYRAC 4 cut(s) 1045, 1155, 2645, 3657
HindII GTYRAC 4 cut(s) 1045, 1155, 2645, 3657
HindIII AAGCTT 1 cut(s) 2572
HpaII CCGG 7 cut(s) 1796, 1949, 2870, 2913, 3092, 3101, 3483
HphI GGTGA 7 cut(s) 910, 1604, 1612, 1954, 2344, 3106, 3773
Hpy166II GTNNAC 6 cut(s) 178, 1045, 1155, 2408, 2645, 3657
Hpy8I GTNNAC 6 cut(s) 178, 1045, 1155, 2408, 2645, 3657
Hpy99I CGWCG 1 cut(s) 1217
HpyCH4IV ACGT 1 cut(s) 657
HpySE526I ACGT 1 cut(s) 657
HspAI GCGC 5 cut(s) 713, 896, 1883, 2627, 3564
Kpn2I TCCGGA 2 cut(s) 1795, 3482
KroI GCCGGC 1 cut(s) 2869
KroNI GCCGGC 1 cut(s) 2871
Ksp22I TGATCA 2 cut(s) 129, 2517
KspI CCGCGG 1 cut(s) 2744
LguI GCTCTTC 1 cut(s) 516
LmnI GCTCC 6 cut(s) 1334, 1724, 2114, 2129, 2798, 3313
LweI GCATC 9 cut(s) 87, 284, 379, 1360, 1528, 1728, 1992, 2716, 3242
MaeI CTAG 9 cut(s) 255, 716, 2102, 2648, 2652, 2864, 2954, 2996, 3161
MaeII ACGT 1 cut(s) 657
MaeIII GTNAC 6 cut(s) 814, 1924, 1960, 3056, 3288, 3779
MfeI CAATTG 2 cut(s) 210, 1442
MflI RGATCY 1 cut(s) 2438
MhlI GDGCHC 2 cut(s) 2410, 2912
MlyI GAGTC 5 cut(s) 61, 183, 2505, 3035, 3668
MmeI TCCRAC 6 cut(s) 199, 230, 1111, 2302, 3528, 3561
MroI TCCGGA 2 cut(s) 1795, 3482
MroNI GCCGGC 1 cut(s) 2869
MroXI GAANNNNTTC 2 cut(s) 1994, 2346
MseI TTAA 6 cut(s) 299, 398, 1007, 1226, 2240, 2903
MslI CAYNNNNRTG 7 cut(s) 530, 764, 2010, 2083, 2540, 2903, 3683
MspA1I CMGCKG 3 cut(s) 103, 2743, 2813
MspCI CTTAAG 1 cut(s) 2239
MspI CCGG 7 cut(s) 1796, 1949, 2870, 2913, 3092, 3101, 3483
MspR9I CCNGG 5 cut(s) 540, 1076, 1949, 3092, 3311
MunI CAATTG 2 cut(s) 210, 1442
Mva1269I GAATGC 2 cut(s) 898, 2080
MvaI CCWGG 3 cut(s) 540, 1076, 3311
MvnI CGCG 3 cut(s) 416, 2743, 2933
NaeI GCCGGC 1 cut(s) 2871
NciI CCSGG 2 cut(s) 1949, 3092
NdeI CATATG 1 cut(s) 3803
NgoMIV GCCGGC 1 cut(s) 2869
NlaIV GGNNCC 4 cut(s) 592, 3315, 3334, 3748
NmuCI GTSAC 5 cut(s) 1924, 1960, 3056, 3288, 3779
NspI RCATGY 1 cut(s) 3633
OliI CACNNNNGTG 1 cut(s) 2083
PaeR7I CTCGAG 1 cut(s) 3513
PagI TCATGA 3 cut(s) 1687, 2514, 3415
PciSI GCTCTTC 1 cut(s) 516
PctI GAATGC 2 cut(s) 898, 2080
PdiI GCCGGC 1 cut(s) 2871
PdmI GAANNNNTTC 2 cut(s) 1994, 2346
PfeI GAWTC 8 cut(s) 18, 667, 858, 879, 980, 2220, 2854, 3127
Pfl23II CGTACG 1 cut(s) 654
PflFI GACNNNGTC 1 cut(s) 1697
PflMI CCANNNNNTGG 3 cut(s) 545, 1853, 1892
PinAI ACCGGT 1 cut(s) 2912
PleI GAGTC 5 cut(s) 61, 183, 2505, 3034, 3668
PpsI GAGTC 5 cut(s) 61, 183, 2505, 3034, 3668
PsiI TTATAA 1 cut(s) 3425
Psp6I CCWGG 3 cut(s) 538, 1074, 3309
PspGI CCWGG 3 cut(s) 538, 1074, 3309
PspLI CGTACG 1 cut(s) 654
PspN4I GGNNCC 4 cut(s) 592, 3315, 3334, 3748
PspPI GGNCC 4 cut(s) 1102, 1573, 2738, 3103
PspXI VCTCGAGB 1 cut(s) 3513
PstNI CAGNNNCTG 1 cut(s) 929
PsuI RGATCY 1 cut(s) 2438
PsyI GACNNNGTC 1 cut(s) 1697
PvuII CAGCTG 1 cut(s) 2813
RsaI GTAC 6 cut(s) 185, 296, 643, 656, 1560, 3446
RsaNI GTAC 6 cut(s) 184, 295, 642, 655, 1559, 3445
RseI CAYNNNNRTG 7 cut(s) 530, 764, 2010, 2083, 2540, 2903, 3683
SacII CCGCGG 1 cut(s) 2744
SalI GTCGAC 1 cut(s) 3655
SapI GCTCTTC 1 cut(s) 516
SaqAI TTAA 6 cut(s) 299, 398, 1007, 1226, 2240, 2903
Sau96I GGNCC 4 cut(s) 1102, 1573, 2738, 3103
ScaI AGTACT 1 cut(s) 296
SchI GAGTC 5 cut(s) 61, 183, 2505, 3035, 3668
ScrFI CCNGG 5 cut(s) 540, 1076, 1949, 3092, 3311
SduI GDGCHC 2 cut(s) 2410, 2912
SfaNI GCATC 9 cut(s) 87, 284, 379, 1360, 1528, 1728, 1992, 2716, 3242
SfcI CTRYAG 1 cut(s) 1241
Sfr274I CTCGAG 1 cut(s) 3513
Sfr303I CCGCGG 1 cut(s) 2744
SgrBI CCGCGG 1 cut(s) 2744
SinI GGWCC 3 cut(s) 1102, 1573, 3103
SlaI CTCGAG 1 cut(s) 3513
SmiMI CAYNNNNRTG 7 cut(s) 530, 764, 2010, 2083, 2540, 2903, 3683
SmlI CTYRAG 5 cut(s) 1097, 1404, 1419, 2239, 3513
SmoI CTYRAG 5 cut(s) 1097, 1404, 1419, 2239, 3513
SpeI ACTAGT 1 cut(s) 3160
SspI AATATT 2 cut(s) 1315, 1971
SspMI CTAG 9 cut(s) 255, 716, 2102, 2648, 2652, 2864, 2954, 2996, 3161
StyD4I CCNGG 5 cut(s) 538, 1074, 1947, 3090, 3309
StyI CCWWGG 3 cut(s) 1569, 1886, 1916
TaiI ACGT 1 cut(s) 660
TatI WGTACW 2 cut(s) 294, 1558
TauI GCSGC 1 cut(s) 2743
TfiI GAWTC 8 cut(s) 18, 667, 858, 879, 980, 2220, 2854, 3127
Tru1I TTAA 6 cut(s) 299, 398, 1007, 1226, 2240, 2903
Tru9I TTAA 6 cut(s) 299, 398, 1007, 1226, 2240, 2903
TscAI CASTG 9 cut(s) 507, 1144, 1250, 2425, 2491, 2689, 3013, 3196, 3243
TseFI GTSAC 5 cut(s) 1924, 1960, 3056, 3288, 3779
Tsp45I GTSAC 5 cut(s) 1924, 1960, 3056, 3288, 3779
TspGWI ACGGA 3 cut(s) 201, 629, 1230
TspRI CASTG 9 cut(s) 507, 1144, 1250, 2425, 2491, 2689, 3013, 3196, 3243
Tth111I GACNNNGTC 1 cut(s) 1697
Van91I CCANNNNNTGG 3 cut(s) 545, 1853, 1892
Vha464I CTTAAG 1 cut(s) 2239
VneI GTGCAC 1 cut(s) 2406
VpaK11BI GGWCC 3 cut(s) 1102, 1573, 3103
XapI RAATTY 7 cut(s) 966, 1031, 1503, 2346, 3012, 3419, 3586
XceI RCATGY 1 cut(s) 3633
XcmI CCANNNNNNNNNTGG 3 cut(s) 172, 1531, 1709
XhoI CTCGAG 1 cut(s) 3513
XmiI GTMKAC 1 cut(s) 3656
XmnI GAANNNNTTC 2 cut(s) 1994, 2346
XspI CTAG 9 cut(s) 255, 716, 2102, 2648, 2652, 2864, 2954, 2996, 3161
ZrmI AGTACT 1 cut(s) 296
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.