MD15G1196000.v1.1

ABC transporter B family member

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0000317
Physical Location & Seq
Reverse (-)
15561398 .. 15568435
7038 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1196000.v1.1.491

Sequence Viewer

Length: 3777 bp
ATGAGTAAAATGAACTCTCCTGAGAAGAATAATGAGCATATGGTTGCAGAGAAAGGAGATGAGAGCAGCAAAGGAGTTATAACTAAAGGAAGAAAATATTCAGTAGTGAAAATATTCAGATATGGTGATTGGGTTGATGTGGTGCTGATGGTGCTGGGTACAGTGGGAGCAATAGGAGATGGGATGTCTACAAATTGCTTGCTGTTGTTCGTTAGCCGTCTTATGAACAATTTGGGGTATGGTCAGACCCAGCCGAAAAATCATGGGAATAATTGGATGGACGAGGTTGAAAAGTGCAGCTTAGACTTTGTATACTTGGGATTAGCAGTAATGCTGGTGGCTTTTTTGGAGGGTTATTGTTGGAGCAAAACCAGTGAGAGGCAGGTGCTGAAGATTCGTTACAAGTACTTGGAAGCTGTTCTAAGGCAGGAGGTTGGGTTTTTCGATTCACAAGAAGCCACTACTTCGGAGGTCATCAATACTATATCAAAAGACACTTCTCTCATACAAGAAGTTCTAAGTGAAAAGGTGCCAACATTCGTTATGCACTCCTCCGTTTTCATCTCCGGCCTGGCCTTCTCCACCTACTTGTCATGGAGACTAGCCTTGGTAGCATTCCCCACACTACTTCTCCTAATCATACCAGGAATGATATATGGGAAGTACCTCATGTACTTGTCGAAAAAATCGTACAAAGAGTACGGAAAAGCAAACACAATAGTAGAGCAAGCATTGAGCTCCATTAAAACTGTTTATTCGTTCACTGCCGAGAGGAGAATTGTGGACAGATATTCAGCAATACTGGAGAGGACGAGCAGGCTGGGGATAAAGCAAGGAATTGCAAAAGGGTTGGCTGTTGGAAGCACGGGGCTTTCTTTTGCGATATGGGGATTTCTTGCTTGGTATGGGAGCCATTTGGTCATGTATAAAGGCGAAAGTGGTGGGAGGATTTATGCGGCTGGCATCTCTTTCGTATTGAGTGGACTATCCCTGGGAATGGCACTTCCGGATTTGAGGTACTTCACAGAAGCTGCTGTTGCCGCCACCAGAATATTCGACAGGATTGACCGGAAACCCTTGATTGATGGTGAAGACACCAAGGGAGTAGTGCTAGACAACATTAGAGGCGAGCTAGAATTCATTGATGTCAAGTTTACATACTCCTCTCGTCCCAATTCGATGGTCCTCAAAGATTTCAACCTCAAAGTTGAAGCTGGGAGGACCATTGCTCTTGTAGGTGCAAGTGGAAGTGGAAAATCCACGGCAATCGCGTTGCTGCAACGCTTTTACGATGCGGATGATGGCGTTGTTCGTGTTGATGGTGTTGATATAAGAACGCTTCAGTTAAATTGGATAAGATCCAAAATGGGACTTGTGAGCCAAGAACATGCATTGTTTGGGACATCAATAAAGGAAAATATTATGTTTGGTAAGCTTGATGCTAATATGGATGAAGTCACAGCTGCAGCTATGGCAGCCAATGCTCATAATTTCATAAGGCAACTGCCAGAGGGGTACGAGACCAAGATTGGAGAGAGAGGAGCTCTTTTATCTGGTGGACAAAAGCAGCGGATAGCCATTGCTAGAGCAATCATCAAGAACCCTGTGATTCTCCTACTTGATGAAGCAACAAGTGCTCTTGACTCTGAATCAGAGGCTTTGGTCCAAAATGCTCTTGATCAAGCCTCCATGGGAAGAACCACATTGGTTGTTGCACACAAGCTTTCCACAGTTCGAAATGCAGACCTAATTGCAGTGGTGGGTGGTGGTTGCATCATCGAAATAGGCTCCCACAACGACCTCATCAACCGCCAAAACGGGCACTATGCAAAGCTAGCAAAGCTGCAGAGGCAGCTCAGTGCTTTTGACAACGTTGAGCAAGAACAAATTTCGGTGTCTTCAGTCAGTAGAAGCAGCGCTGGCCGGCTAAGCACGCCAAGATCAAGCCCTGCACCAGCGTTTGCAAAATCGCCATTACCTAATGAGACCCTACAACTTGTGTCACATCCTCCGACTTCCTTTTACCGGCTTCTCTCTTTAAATTCCCCGGAATGGAAGCAAGGCCTAATAGGAAGCCTCTCTGCCATAGCGTTCGGGTCAGTTCAACCTATCTATGCCCTAACCATAGGTGGCATGATATCCGCTTTCTTTGTGCAAAGCCATGAGGAAATGCGCGCTAGAATTCGCACATACTCCTTGATTTTCTGTGCACTTTCCTTAATTTCCATGACTTTGAATCTCTTCCAACACTACAATTTTGCTTACATGGGTGAGCAGCTCACGAAAAGAATCCGATTACGAATGCTTCAAAAGATCTTGACTTTTGAAACGGCTTGGTTTGATGAGGAGCAGAACTCGAGCGGGGCATTGTGTTCGAGATTGAGCAATGAGGCTTCCATGGTTAAGTCCCTCGTTGCAGACAGGGTGTCCTTATTAGTCCAAACCACTTCAGCTGTCACAATAGCCATGATCCTGGGGCTCATCGTGGCATGGAAGCTAGCACTCGTTATGATCGCAGTCCAACCACTCACGATCCTTTGCTTCTACACCAAGAAAGTCCTGCTCTCTAGTCTCTCAGCCAACTTCATCAAAGCACAAAACCACAGCACTCAAATTGCAGTTGAATCAGTGTACAACCATAGAATTGTGACATCATTCGGAAGCGTGGGAAAAGTACTTCAACTGTTCGATGAGGCTCAGGAGGCACCGCGGAAAGAAGCAAGTAAGAAGGCATGGCTAGCTGGGATTGGAATGGGGTCAGCTCAGTGCCTAACATTTATGTCATGGGCATTGGACTTCTGGTATGGTGGTACATTAGTGAAAAAAGGGCAAATATCAGCTGGGGATGTGTTCAAAACATTTTTCATATTGGTCAGCACTGGTAAAGTTATAGCTGAAGCTGGAAGCATGACTTCTGATTTGGCCAAGGGTTCGACTGCAGTTGCATCCGTGTTTGAGATTCTTGACCGGCATTCACTAATTTCAGGCTCTAATAATGCTGGGGACGGAGATAGTAATAATGGAAACGGGATCAAATTGGAGAAAGTGACTGGAAGGATTGAATTGAAGAAGGTTGATTTTGCATACCCCAGCAGGCCAGAGACATTAGTGCTGCGCCAATTTAGCTTAGAGGTGAAGCCAGGCACAAGCACTGGACTTGTTGGGACAAGTGGATGTGGAAAATCAACTGTGATTGGATTGATACAAAGATTTTATGATGTAGAGAGGGGATCAGTGAAGGTGGATGGGGTTGACATAAGGGATCTAGATATTCAATGGTACCGGAGGCACACGGCCCTTGTTAGTCAAGAGCCGGTGATATACTCTGGTACCATCCGAGACAACATCGTGTTTGGGAAACTTGATGTGCCAGAAAATGAGGTCACAGTAGCTGCCAGAGCCGCCAATGCGCACGAATTTATCTCGTCACTTAAGGATGGGTATGACACTGAGTGCGGCGAAAGAGGGGTGCAGCTATCAGGAGGACAAAAGCAAAGGATTGCAATTGCAAGAGCAATACTTAGGAATCCAACCGTATTGTTGCTAGATGAGGCAACAAGTGCACTTGATGTGCAATCAGAGCAACTTGTGCAGGAAGCATTAGATCGCATTATGGTTGGAAGGACAACAATTGTGATAGCACACAGGCTCAACACCATAAAAAACCTAGATATGATTGCATTTGTTGCAGATGGGAAGGTGGTGGAAAAAGGCACATATGCTCAGCTCAAGCAAAAGCGTGGTGCGTTTTTTAACCTTGCCACGTGCCAGAGATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko02010 ko04976 ko05206 ko05226 map02010 map04976 map05206 map05226
Pfam Domains
Protein Families
ABC_membrane ABC_tran

Protein Analysis

1259

Amino Acids

137.92

Weight (kDa)

9.02

Isoelectric Point (pI)

29.87

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ABC_membrane PF00664 53 - 312 2.6e-46 ABC transporter transmembrane region
ABC_tran PF00005 396 - 545 2.3e-30 ABC transporter
ABC_membrane PF00664 691 - 959 2.3e-47 ABC transporter transmembrane region
ABC_tran PF00005 1038 - 1187 1e-30 ABC transporter
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000317)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G28345 AT3G28360 AT3G28380 AT3G28390 AT3G28415 AT3G28415
fragaria_vesca FvH4_1g06060 FvH4_3g21880 FvH4_4g25260 FvH4_6g49750 FvH4_6g49750 FvH4_6g49750 FvH4_6g49750 FvH4_6g49760
malus_domestica MD00G1221200.v1.1 MD02G1065200.v1.1 MD03G1211200.v1.1 MD09G1041500.v1.1 MD09G1041600.v1.1 MD09G1041900.v1.1 MD15G1196000.v1.1 MD16G1105600.v1.1 MD17G1042800.v1.1
prunus_persica Prupe.1G241000_v2.0.a1 Prupe.3G275900_v2.0.a1 Prupe.3G276100_v2.0.a1 Prupe.3G276100_v2.0.a1 Prupe.4G204300_v2.0.a1 Prupe.7G220300_v2.0.a1
pyrus_communis pycom111g03260 pycom16g08980 pycom17g03840
rosa_chinensis RchiOBHm_Chr2g0091821 RchiOBHm_Chr2g0169781 RchiOBHm_Chr2g0169791 RchiOBHm_Chr2g0169801 RchiOBHm_Chr2g0169841 RchiOBHm_Chr2g0169851 RchiOBHm_Chr2g0169861 RchiOBHm_Chr4g0432501 RchiOBHm_Chr5g0037681 RchiOBHm_Chr5g0037691
rosa_laevigata RLG00000006836 RLG00000016254 RLG00000021903 RLG00000021904 RLG00000021905 RLG00000021906 RLG00000021907 RLG00000021908 RLG00000033801
rosa_multiflora Rmu_sc0001084.1_g000003 Rmu_sc0021402.1_g000003 Rmu_ssc0000389.1_g000016 Rmu_ssc0000389.1_g000019 Rmu_ssc0000389.1_g000024 Rmu_ssc0000389.1_g000027
rosa_roxburghii Rroxscaffold_1G00043250 Rroxscaffold_2G00081770 Rroxscaffold_2G00081780 Rroxscaffold_2G00081790 Rroxscaffold_2G00149710 Rroxscaffold_5G00373960
rosa_rugosa Rorug02G0021300 Rorug02G0543400.1 Rorug02G0543500.1 Rorug02G0543600.1 Rorug02G0543700.1 Rorug02G0543800 Rorug02G0543900 Rorug02G0544000 Rorug04G0259900.1 Rorug05G0166300 Rorug05G0166300
rosa_samantha Rh2AG065900 Rh2AG615300 Rh2AG615400 Rh2AG615500 Rh2AG615600 Rh2AG615700 Rh2AG615800 Rh2AG616000 Rh2AG616200 Rh2BG627600 Rh2BG627700 Rh2BG627800 Rh2BG627900 Rh2BG628000 Rh2BG628100 Rh2CG596900 Rh2CG597000 Rh2CG597100 Rh2CG597200 Rh2DG065000 Rh2DG638400 Rh2DG638600 Rh2DG638700 Rh2DG638800 Rh2DG638900 Rh2DG639000 Rh4AG314700 Rh4BG322400 Rh4CG337600 Rh4DG318300 Rh5AG255800 Rh5BG257900 Rh5CG291300
rosa_wichuraiana Rw0G002540 Rw2G005480 Rw2G051010 Rw2G051020 Rw2G051030 Rw2G051040 Rw4G027360 Rw5G023710 Rw5G024000

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 80
AarI CACCTGC 1 cut(s) 373
Acc16I TGCGCA 1 cut(s) 3411
Acc36I ACCTGC 1 cut(s) 373
Acc65I GGTACC 2 cut(s) 3279, 3329
AccB1I GGYRCC 4 cut(s) 529, 2704, 3279, 3329
AccBSI CCGCTC 1 cut(s) 2361
AccI GTMKAC 2 cut(s) 188, 312
AccII CGCG 3 cut(s) 1271, 2175, 2710
AccIII TCCGGA 1 cut(s) 1006
AclI AACGTT 1 cut(s) 1872
AclWI GGATC 6 cut(s) 1353, 2464, 2527, 3038, 3238, 3270
AcoI YGGCCR 2 cut(s) 1921, 2922
AcsI RAATTY 5 cut(s) 1136, 1887, 2041, 2181, 3416
AcuI CTGAAG 5 cut(s) 410, 1325, 1884, 2433, 2916
AcvI CACGTG 1 cut(s) 3765
AdeI CACNNNGTG 1 cut(s) 3453
AfeI AGCGCT 1 cut(s) 1918
AfiI CCNNNNNNNGG 4 cut(s) 378, 997, 2025, 2052
AflII CTTAAG 1 cut(s) 3431
AhdI GACNNNNNGTC 1 cut(s) 2425
AjnI CCWGG 5 cut(s) 570, 643, 990, 2472, 3139
Alw21I GWGCWC 5 cut(s) 740, 1546, 1639, 2212, 3565
Alw26I GTCTC 6 cut(s) 592, 1514, 1979, 2576, 3095, 3333
Alw44I GTGCAC 2 cut(s) 2208, 3561
AlwI GGATC 6 cut(s) 1353, 2464, 2527, 3038, 3238, 3270
AlwNI CAGNNNCTG 3 cut(s) 388, 1031, 3392
Ama87I CYCGRG 1 cut(s) 2356
Aor13HI TCCGGA 1 cut(s) 1006
Aor51HI AGCGCT 1 cut(s) 1918
AoxI GGCC 7 cut(s) 568, 573, 1921, 2062, 2922, 3095, 3294
ApaLI GTGCAC 2 cut(s) 2208, 3561
ApoI RAATTY 5 cut(s) 1136, 1887, 2041, 2181, 3416
ArsI GACNNNNNNTTYG 2 cut(s) 2902, 2934
Asp700I GAANNNNTTC 4 cut(s) 97, 113, 417, 2240
Asp718I GGTACC 2 cut(s) 3279, 3329
AspLEI GCGC 5 cut(s) 1919, 2175, 2177, 3117, 3412
AspS9I GGNCC 4 cut(s) 1183, 1221, 1663, 3295
AsuC2I CCSGG 1 cut(s) 2048
AsuHPI GGTGA 5 cut(s) 137, 1100, 2282, 3145, 3328
AsuII TTCGAA 1 cut(s) 1735
AsuNHI GCTAGC 3 cut(s) 1834, 2497, 2737
AvaI CYCGRG 1 cut(s) 2356
AvaII GGWCC 3 cut(s) 1183, 1221, 1663
BaeGI GKGCMC 3 cut(s) 1824, 2212, 3565
BaeI ACNNNNGTAYC 4 cut(s) 2802, 2835, 3313, 3346
BalI TGGCCA 1 cut(s) 2924
BanI GGYRCC 4 cut(s) 529, 2704, 3279, 3329
BanII GRGCYC 3 cut(s) 740, 1546, 2481
BarI GAAGNNNNNNTAC 2 cut(s) 383, 415
BbrPI CACGTG 1 cut(s) 3765
BbsI GAAGAC 2 cut(s) 1098, 1890
Bbv12I GWGCWC 5 cut(s) 740, 1546, 1639, 2212, 3565
BceAI ACGGC 4 cut(s) 201, 1278, 2346, 3309
BcgI CGANNNNNNTGC 4 cut(s) 952, 986, 2355, 2389
BciT130I CCWGG 5 cut(s) 572, 645, 992, 2474, 3141
BclI TGATCA 1 cut(s) 1678
BcnI CCSGG 1 cut(s) 2048
BcoDI GTCTC 6 cut(s) 592, 1514, 1979, 2576, 3095, 3333
BfmI CTRYAG 3 cut(s) 1464, 1844, 2937
BfoI RGCGCY 1 cut(s) 1920
BfrI CTTAAG 1 cut(s) 3431
BfuAI ACCTGC 1 cut(s) 373
BglII AGATCT 1 cut(s) 2313
BlpI GCTNAGC 2 cut(s) 1928, 3723
BmcAI AGTACT 2 cut(s) 407, 2676
Bme1390I CCNGG 6 cut(s) 572, 645, 992, 2048, 2474, 3141
Bme18I GGWCC 3 cut(s) 1183, 1221, 1663
BmeRI GACNNNNNGTC 1 cut(s) 2425
BmeT110I CYCGRG 1 cut(s) 2356
BmgT120I GGNCC 4 cut(s) 1183, 1221, 1663, 3295
BmiI GGNNCC 6 cut(s) 531, 911, 1789, 2706, 3281, 3331
BmrFI CCNGG 6 cut(s) 572, 645, 992, 2048, 2474, 3141
BmsI GCATC 5 cut(s) 972, 1282, 1429, 1782, 2954
BmtI GCTAGC 3 cut(s) 1838, 2501, 2741
BpiI GAAGAC 2 cut(s) 1098, 1890
BplI GAGNNNNNCTC 4 cut(s) 1528, 1560, 2339, 2371
BpmI CTGGAG 1 cut(s) 824
Bpu10I CCTNAGC 1 cut(s) 2697
Bpu1102I GCTNAGC 2 cut(s) 1928, 3723
Bpu14I TTCGAA 1 cut(s) 1735
BpuEI CTTGAG 1 cut(s) 3713
BpuMI CCSGG 1 cut(s) 2048
BsaAI YACGTR 1 cut(s) 3765
BsaI GGTCTC 2 cut(s) 1514, 1979
BsaWI WCCGGW 3 cut(s) 1006, 1068, 3282
BsaXI ACNNNNNCTCC 4 cut(s) 422, 452, 615, 645
Bsc4I CCNNNNNNNGG 4 cut(s) 378, 997, 2025, 2052
Bse118I RCCGGY 4 cut(s) 1923, 2025, 2967, 3313
Bse1I ACTGG 5 cut(s) 372, 807, 2884, 3055, 3157
Bse3DI GCAATG 3 cut(s) 1224, 1578, 2392
BseAI TCCGGA 1 cut(s) 1006
BseBI CCWGG 5 cut(s) 572, 645, 992, 2474, 3141
BseLI CCNNNNNNNGG 4 cut(s) 378, 997, 2025, 2052
BseMI GCAATG 3 cut(s) 1224, 1578, 2392
BseMII CTCAG 7 cut(s) 12, 1870, 2589, 2711, 2777, 3441, 3737
BseNI ACTGG 5 cut(s) 372, 807, 2884, 3055, 3157
BsePI GCGCGC 1 cut(s) 2173
BseRI GAGGAG 5 cut(s) 541, 787, 1153, 1554, 2360
BseSI GKGCMC 3 cut(s) 1824, 2212, 3565
BseYI CCCAGC 8 cut(s) 154, 249, 820, 1214, 2741, 2840, 2999, 3089
BsgI GTGCAG 4 cut(s) 316, 1935, 3491, 3611
Bsh1236I CGCG 3 cut(s) 1271, 2175, 2710
BshFI GGCC 7 cut(s) 570, 575, 1923, 2064, 2924, 3097, 3296
BshNI GGYRCC 4 cut(s) 529, 2704, 3279, 3329
BsiHKAI GWGCWC 5 cut(s) 740, 1546, 1639, 2212, 3565
BsiHKCI CYCGRG 1 cut(s) 2356
BsiSI CCGG 9 cut(s) 567, 1007, 1069, 1924, 2026, 2048, 2968, 3283, 3314
BslFI GGGAC 6 cut(s) 1155, 1383, 1414, 2392, 3017, 3178
BslI CCNNNNNNNGG 4 cut(s) 378, 997, 2025, 2052
BsmAI GTCTC 6 cut(s) 592, 1514, 1979, 2576, 3095, 3333
BsmFI GGGAC 6 cut(s) 1155, 1383, 1414, 2392, 3017, 3178
BsmI GAATGC 3 cut(s) 614, 2307, 2971
BsnI GGCC 7 cut(s) 570, 575, 1923, 2064, 2924, 3097, 3296
Bso31I GGTCTC 2 cut(s) 1514, 1979
BsoBI CYCGRG 1 cut(s) 2356
Bsp119I TTCGAA 1 cut(s) 1735
Bsp1286I GDGCHC 7 cut(s) 740, 1546, 1639, 1824, 2212, 2481, 3565
Bsp13I TCCGGA 1 cut(s) 1006
Bsp1407I TGTACA 1 cut(s) 2631
Bsp1720I GCTNAGC 2 cut(s) 1928, 3723
Bsp19I CCATGG 2 cut(s) 1689, 2397
BspANI GGCC 7 cut(s) 570, 575, 1923, 2064, 2924, 3097, 3296
BspCNI CTCAG 7 cut(s) 13, 1869, 2588, 2710, 2776, 3442, 3736
BspEI TCCGGA 1 cut(s) 1006
BspFNI CGCG 3 cut(s) 1271, 2175, 2710
BspLI GGNNCC 6 cut(s) 531, 911, 1789, 2706, 3281, 3331
BspMAI CTGCAG 3 cut(s) 1468, 1848, 2941
BspMI ACCTGC 1 cut(s) 373
BspOI GCTAGC 3 cut(s) 1838, 2501, 2741
BspPI GGATC 6 cut(s) 1353, 2464, 2527, 3038, 3238, 3270
BspT104I TTCGAA 1 cut(s) 1735
BspT107I GGYRCC 4 cut(s) 529, 2704, 3279, 3329
BspTI CTTAAG 1 cut(s) 3431
BspTNI GGTCTC 2 cut(s) 1514, 1979
BsrBI CCGCTC 1 cut(s) 2361
BsrDI GCAATG 3 cut(s) 1224, 1578, 2392
BsrFI RCCGGY 4 cut(s) 1923, 2025, 2967, 3313
BsrGI TGTACA 1 cut(s) 2631
BsrI ACTGG 5 cut(s) 372, 807, 2884, 3055, 3157
BssAI RCCGGY 4 cut(s) 1923, 2025, 2967, 3313
BssHII GCGCGC 1 cut(s) 2173
BssNAI GTATAC 1 cut(s) 313
BssT1I CCWWGG 5 cut(s) 606, 1098, 1689, 2397, 2925
Bst1107I GTATAC 1 cut(s) 313
Bst2UI CCWGG 5 cut(s) 572, 645, 992, 2474, 3141
Bst4CI ACNGT 7 cut(s) 163, 751, 1732, 2685, 3190, 3388, 3535
Bst6I CTCTTC 1 cut(s) 2246
BstAFI CTTAAG 1 cut(s) 3431
BstAPI GCANNNNNTGC 5 cut(s) 1481, 1634, 3560, 3589, 3686
BstAUI TGTACA 1 cut(s) 2631
BstBAI YACGTR 1 cut(s) 3765
BstBI TTCGAA 1 cut(s) 1735
BstDSI CCRYGG 4 cut(s) 1260, 1689, 2397, 2708
BstFNI CGCG 3 cut(s) 1271, 2175, 2710
BstH2I RGCGCY 1 cut(s) 1920
BstHHI GCGC 5 cut(s) 1919, 2175, 2177, 3117, 3412
BstMAI GTCTC 6 cut(s) 592, 1514, 1979, 2576, 3095, 3333
BstNI CCWGG 5 cut(s) 572, 645, 992, 2474, 3141
BstNSI RCATGY 1 cut(s) 1391
BstSCI CCNGG 6 cut(s) 570, 643, 990, 2046, 2472, 3139
BstSFI CTRYAG 3 cut(s) 1464, 1844, 2937
BstSLI GKGCMC 3 cut(s) 1824, 2212, 3565
BstUI CGCG 3 cut(s) 1271, 2175, 2710
BstV2I GAAGAC 2 cut(s) 1098, 1890
BstX2I RGATCY 3 cut(s) 1358, 2313, 3262
BstXI CCANNNNNNTGG 2 cut(s) 1180, 2473
BstYI RGATCY 3 cut(s) 1358, 2313, 3262
BstZ17I GTATAC 1 cut(s) 313
BsuRI GGCC 7 cut(s) 570, 575, 1923, 2064, 2924, 3097, 3296
BtgI CCRYGG 4 cut(s) 1260, 1689, 2397, 2708
BtsI GCAGTG 2 cut(s) 762, 1761
BveI ACCTGC 1 cut(s) 373
CaiI CAGNNNCTG 3 cut(s) 388, 1031, 3392
CfoI GCGC 5 cut(s) 1919, 2175, 2177, 3117, 3412
Cfr10I RCCGGY 4 cut(s) 1923, 2025, 2967, 3313
Cfr13I GGNCC 4 cut(s) 1183, 1221, 1663, 3295
Cfr42I CCGCGG 1 cut(s) 2711
CspCI CAANNNNNGTGG 2 cut(s) 1690, 1725
DraI TTTAAA 1 cut(s) 2040
DraIII CACNNNGTG 1 cut(s) 3453
DriI GACNNNNNGTC 1 cut(s) 2425
EaeI YGGCCR 2 cut(s) 1921, 2922
Eam1104I CTCTTC 1 cut(s) 2246
Eam1105I GACNNNNNGTC 1 cut(s) 2425
EarI CTCTTC 1 cut(s) 2246
Ecl136II GAGCTC 2 cut(s) 738, 1544
Eco130I CCWWGG 5 cut(s) 606, 1098, 1689, 2397, 2925
Eco147I AGGCCT 1 cut(s) 2064
Eco24I GRGCYC 3 cut(s) 740, 1546, 2481
Eco31I GGTCTC 2 cut(s) 1514, 1979
Eco32I GATATC 1 cut(s) 2139
Eco47I GGWCC 3 cut(s) 1183, 1221, 1663
Eco47III AGCGCT 1 cut(s) 1918
Eco53kI GAGCTC 2 cut(s) 738, 1544
Eco57I CTGAAG 5 cut(s) 410, 1325, 1884, 2433, 2916
Eco72I CACGTG 1 cut(s) 3765
Eco88I CYCGRG 1 cut(s) 2356
EcoICRI GAGCTC 2 cut(s) 738, 1544
EcoRI GAATTC 2 cut(s) 1136, 2181
EcoRII CCWGG 5 cut(s) 570, 643, 990, 2472, 3139
EcoRV GATATC 1 cut(s) 2139
EcoT14I CCWWGG 5 cut(s) 606, 1098, 1689, 2397, 2925
EcoT22I ATGCAT 1 cut(s) 1393
EcoT38I GRGCYC 3 cut(s) 740, 1546, 2481
ErhI CCWWGG 5 cut(s) 606, 1098, 1689, 2397, 2925
FalI AAGNNNNNCTT 4 cut(s) 284, 316, 2896, 2928
FaqI GGGAC 6 cut(s) 1155, 1383, 1414, 2392, 3017, 3178
FauI CCCGC 1 cut(s) 2354
FauNDI CATATG 2 cut(s) 39, 3718
FbaI TGATCA 1 cut(s) 1678
FblI GTMKAC 2 cut(s) 188, 312
FriOI GRGCYC 3 cut(s) 740, 1546, 2481
FspAI RTGCGCAY 1 cut(s) 3411
FspI TGCGCA 1 cut(s) 3411
GlaI GCGC 5 cut(s) 1918, 2174, 2176, 3116, 3411
GsaI CCCAGC 8 cut(s) 158, 253, 824, 1218, 2745, 2844, 3003, 3093
GsuI CTGGAG 1 cut(s) 824
HaeII RGCGCY 1 cut(s) 1920
HaeIII GGCC 7 cut(s) 570, 575, 1923, 2064, 2924, 3097, 3296
HapII CCGG 9 cut(s) 567, 1007, 1069, 1924, 2026, 2048, 2968, 3283, 3314
HhaI GCGC 5 cut(s) 1919, 2175, 2177, 3117, 3412
Hin6I GCGC 5 cut(s) 1917, 2173, 2175, 3115, 3410
HinP1I GCGC 5 cut(s) 1917, 2173, 2175, 3115, 3410
HincII GTYRAC 1 cut(s) 3253
HindII GTYRAC 1 cut(s) 3253
HindIII AAGCTT 2 cut(s) 1433, 1721
HpaII CCGG 9 cut(s) 567, 1007, 1069, 1924, 2026, 2048, 2968, 3283, 3314
HphI GGTGA 5 cut(s) 137, 1100, 2282, 3145, 3328
HpyAV CCTTC 7 cut(s) 586, 2722, 3048, 3064, 3232, 3615, 3691
HpyCH4III ACNGT 7 cut(s) 163, 751, 1732, 2685, 3190, 3388, 3535
HpyCH4IV ACGT 2 cut(s) 1872, 3764
HpySE526I ACGT 2 cut(s) 1872, 3764
HspAI GCGC 5 cut(s) 1917, 2173, 2175, 3115, 3410
Kpn2I TCCGGA 1 cut(s) 1006
KpnI GGTACC 2 cut(s) 3283, 3333
KroI GCCGGC 1 cut(s) 1923
KroNI GCCGGC 1 cut(s) 1925
Ksp22I TGATCA 1 cut(s) 1678
KspI CCGCGG 1 cut(s) 2711
LmnI GCTCC 7 cut(s) 167, 363, 743, 909, 1541, 1793, 2347
LweI GCATC 5 cut(s) 972, 1282, 1429, 1782, 2954
MaeII ACGT 2 cut(s) 1872, 3764
MaeIII GTNAC 8 cut(s) 398, 1456, 2001, 2455, 2647, 3046, 3382, 3426
MbiI CCGCTC 1 cut(s) 2361
MboII GAAGA 8 cut(s) 37, 102, 403, 1103, 1707, 1890, 2233, 3079
MfeI CAATTG 2 cut(s) 3504, 3630
MflI RGATCY 3 cut(s) 1358, 2313, 3262
MhlI GDGCHC 7 cut(s) 740, 1546, 1639, 1824, 2212, 2481, 3565
MlsI TGGCCA 1 cut(s) 2924
MluNI TGGCCA 1 cut(s) 2924
MlyI GAGTC 1 cut(s) 1637
MmeI TCCRAC 7 cut(s) 341, 838, 2036, 2269, 2545, 3554, 3598
Mox20I TGGCCA 1 cut(s) 2924
Mph1103I ATGCAT 1 cut(s) 1393
MroI TCCGGA 1 cut(s) 1006
MroNI GCCGGC 1 cut(s) 1923
MroXI GAANNNNTTC 4 cut(s) 97, 113, 417, 2240
MscI TGGCCA 1 cut(s) 2924
MseI TTAA 7 cut(s) 744, 1346, 2039, 2219, 2403, 3432, 3753
Msp20I TGGCCA 1 cut(s) 2924
MspA1I CMGCKG 5 cut(s) 1463, 1570, 2453, 2710, 2840
MspCI CTTAAG 1 cut(s) 3431
MspI CCGG 9 cut(s) 567, 1007, 1069, 1924, 2026, 2048, 2968, 3283, 3314
MspR9I CCNGG 6 cut(s) 572, 645, 992, 2048, 2474, 3141
MunI CAATTG 2 cut(s) 3504, 3630
Mva1269I GAATGC 3 cut(s) 614, 2307, 2971
MvaI CCWGG 5 cut(s) 572, 645, 992, 2474, 3141
MvnI CGCG 3 cut(s) 1271, 2175, 2710
NaeI GCCGGC 1 cut(s) 1925
NciI CCSGG 1 cut(s) 2048
NcoI CCATGG 2 cut(s) 1689, 2397
NdeI CATATG 2 cut(s) 39, 3718
NgoMIV GCCGGC 1 cut(s) 1923
NheI GCTAGC 3 cut(s) 1834, 2497, 2737
NlaIV GGNNCC 6 cut(s) 531, 911, 1789, 2706, 3281, 3331
NmeAIII GCCGAG 1 cut(s) 793
NmuCI GTSAC 7 cut(s) 1456, 2001, 2455, 2647, 3046, 3382, 3426
NsbI TGCGCA 1 cut(s) 3411
NsiI ATGCAT 1 cut(s) 1393
NspI RCATGY 1 cut(s) 1391
NspV TTCGAA 1 cut(s) 1735
PaeR7I CTCGAG 1 cut(s) 2356
PaqCI CACCTGC 1 cut(s) 373
PasI CCCWGGG 1 cut(s) 991
PauI GCGCGC 1 cut(s) 2173
PceI AGGCCT 1 cut(s) 2064
PcsI WCGNNNNNNNCGW 2 cut(s) 686, 1268
PctI GAATGC 3 cut(s) 614, 2307, 2971
PdiI GCCGGC 1 cut(s) 1925
PdmI GAANNNNTTC 4 cut(s) 97, 113, 417, 2240
PfeI GAWTC 9 cut(s) 394, 446, 1609, 1649, 2236, 2289, 2624, 2959, 3526
PleI GAGTC 1 cut(s) 1637
PmaCI CACGTG 1 cut(s) 3765
PmlI CACGTG 1 cut(s) 3765
PpsI GAGTC 1 cut(s) 1637
Ppu21I YACGTR 1 cut(s) 3765
PsiI TTATAA 1 cut(s) 80
Psp124BI GAGCTC 2 cut(s) 740, 1546
Psp1406I AACGTT 1 cut(s) 1872
Psp6I CCWGG 5 cut(s) 570, 643, 990, 2472, 3139
PspCI CACGTG 1 cut(s) 3765
PspFI CCCAGC 8 cut(s) 154, 249, 820, 1214, 2741, 2840, 2999, 3089
PspGI CCWGG 5 cut(s) 570, 643, 990, 2472, 3139
PspN4I GGNNCC 6 cut(s) 531, 911, 1789, 2706, 3281, 3331
PspPI GGNCC 4 cut(s) 1183, 1221, 1663, 3295
PspXI VCTCGAGB 1 cut(s) 2356
PstI CTGCAG 3 cut(s) 1468, 1848, 2941
PstNI CAGNNNCTG 3 cut(s) 388, 1031, 3392
PsuI RGATCY 3 cut(s) 1358, 2313, 3262
PteI GCGCGC 1 cut(s) 2173
PvuII CAGCTG 3 cut(s) 1463, 2453, 2840
SacI GAGCTC 2 cut(s) 740, 1546
SacII CCGCGG 1 cut(s) 2711
SaqAI TTAA 7 cut(s) 744, 1346, 2039, 2219, 2403, 3432, 3753
Sau96I GGNCC 4 cut(s) 1183, 1221, 1663, 3295
ScaI AGTACT 2 cut(s) 407, 2676
SchI GAGTC 1 cut(s) 1637
ScrFI CCNGG 6 cut(s) 572, 645, 992, 2048, 2474, 3141
SduI GDGCHC 7 cut(s) 740, 1546, 1639, 1824, 2212, 2481, 3565
SfaNI GCATC 5 cut(s) 972, 1282, 1429, 1782, 2954
SfcI CTRYAG 3 cut(s) 1464, 1844, 2937
Sfr274I CTCGAG 1 cut(s) 2356
Sfr303I CCGCGG 1 cut(s) 2711
SfuI TTCGAA 1 cut(s) 1735
SgrBI CCGCGG 1 cut(s) 2711
SinI GGWCC 3 cut(s) 1183, 1221, 1663
SlaI CTCGAG 1 cut(s) 2356
SmlI CTYRAG 3 cut(s) 2356, 3431, 3728
SmoI CTYRAG 3 cut(s) 2356, 3431, 3728
SseBI AGGCCT 1 cut(s) 2064
SspI AATATT 4 cut(s) 98, 114, 1053, 1420
SstI GAGCTC 2 cut(s) 740, 1546
StuI AGGCCT 1 cut(s) 2064
StyD4I CCNGG 6 cut(s) 570, 643, 990, 2046, 2472, 3139
StyI CCWWGG 5 cut(s) 606, 1098, 1689, 2397, 2925
TaaI ACNGT 7 cut(s) 163, 751, 1732, 2685, 3190, 3388, 3535
TaiI ACGT 2 cut(s) 1875, 3767
TatI WGTACW 4 cut(s) 405, 672, 2631, 2674
TauI GCSGC 4 cut(s) 959, 1043, 3404, 3459
TfiI GAWTC 9 cut(s) 394, 446, 1609, 1649, 2236, 2289, 2624, 2959, 3526
Tru1I TTAA 7 cut(s) 744, 1346, 2039, 2219, 2403, 3432, 3753
Tru9I TTAA 7 cut(s) 744, 1346, 2039, 2219, 2403, 3432, 3753
TseFI GTSAC 7 cut(s) 1456, 2001, 2455, 2647, 3046, 3382, 3426
Tsp45I GTSAC 7 cut(s) 1456, 2001, 2455, 2647, 3046, 3382, 3426
TspDTI ATGAA 9 cut(s) 26, 239, 550, 1129, 1467, 1483, 1638, 2575, 2854
TspGWI ACGGA 4 cut(s) 544, 717, 2938, 3021
Vha464I CTTAAG 1 cut(s) 3431
VneI GTGCAC 2 cut(s) 2208, 3561
VpaK11BI GGWCC 3 cut(s) 1183, 1221, 1663
XapI RAATTY 5 cut(s) 1136, 1887, 2041, 2181, 3416
XbaI TCTAGA 1 cut(s) 3265
XceI RCATGY 1 cut(s) 1391
XhoI CTCGAG 1 cut(s) 2356
XmiI GTMKAC 2 cut(s) 188, 312
XmnI GAANNNNTTC 4 cut(s) 97, 113, 417, 2240
ZrmI AGTACT 2 cut(s) 407, 2676
Zsp2I ATGCAT 1 cut(s) 1393
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.