DNA polymerase
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AanI | TTATAA | 1 cut(s) | 2721 |
| AarI | CACCTGC | 1 cut(s) | 2805 |
| AasI | GACNNNNNNGTC | 2 cut(s) | 1769, 2150 |
| Acc36I | ACCTGC | 2 cut(s) | 1528, 2805 |
| AccB1I | GGYRCC | 2 cut(s) | 1477, 2182 |
| AccI | GTMKAC | 2 cut(s) | 324, 1296 |
| AccII | CGCG | 1 cut(s) | 503 |
| AclI | AACGTT | 1 cut(s) | 753 |
| AclWI | GGATC | 7 cut(s) | 863, 1458, 1559, 1572, 2291, 2765, 2778 |
| AcoI | YGGCCR | 2 cut(s) | 114, 199 |
| AcsI | RAATTY | 6 cut(s) | 697, 1385, 2017, 2450, 2683, 2746 |
| AcuI | CTGAAG | 2 cut(s) | 231, 2693 |
| AcyI | GRCGYC | 1 cut(s) | 149 |
| AfaI | GTAC | 3 cut(s) | 275, 1393, 1539 |
| AfeI | AGCGCT | 1 cut(s) | 1630 |
| AfiI | CCNNNNNNNGG | 5 cut(s) | 123, 126, 297, 2262, 2841 |
| AflII | CTTAAG | 2 cut(s) | 1541, 2216 |
| AflIII | ACRYGT | 1 cut(s) | 2537 |
| AgeI | ACCGGT | 1 cut(s) | 1480 |
| AjiI | CACGTC | 2 cut(s) | 546, 2540 |
| AjnI | CCWGG | 2 cut(s) | 1930, 2145 |
| AjuI | GAANNNNNNNTTGG | 2 cut(s) | 743, 775 |
| AleI | CACNNNNGTG | 1 cut(s) | 1904 |
| Alw26I | GTCTC | 4 cut(s) | 258, 1777, 1864, 2474 |
| AlwI | GGATC | 7 cut(s) | 863, 1458, 1559, 1572, 2291, 2765, 2778 |
| AlwNI | CAGNNNCTG | 1 cut(s) | 56 |
| Aor51HI | AGCGCT | 1 cut(s) | 1630 |
| ApeKI | GCWGC | 1 cut(s) | 1399 |
| ApoI | RAATTY | 6 cut(s) | 697, 1385, 2017, 2450, 2683, 2746 |
| AseI | ATTAAT | 2 cut(s) | 813, 1842 |
| AsiGI | ACCGGT | 1 cut(s) | 1480 |
| AspA2I | CCTAGG | 1 cut(s) | 337 |
| AspLEI | GCGC | 1 cut(s) | 1631 |
| AsuC2I | CCSGG | 1 cut(s) | 1022 |
| AsuHPI | GGTGA | 4 cut(s) | 878, 1066, 1079, 2489 |
| AvaII | GGWCC | 8 cut(s) | 140, 241, 307, 383, 691, 1018, 2199, 2802 |
| AvrII | CCTAGG | 1 cut(s) | 337 |
| BaeGI | GKGCMC | 1 cut(s) | 2599 |
| BalI | TGGCCA | 1 cut(s) | 116 |
| BamHI | GGATCC | 2 cut(s) | 1564, 2770 |
| BanI | GGYRCC | 2 cut(s) | 1477, 2182 |
| BanII | GRGCYC | 1 cut(s) | 169 |
| BauI | CACGAG | 1 cut(s) | 375 |
| BbsI | GAAGAC | 1 cut(s) | 372 |
| BbvI | GCAGC | 1 cut(s) | 1386 |
| BccI | CCATC | 6 cut(s) | 229, 508, 1038, 1064, 1145, 1964 |
| BceAI | ACGGC | 2 cut(s) | 349, 1147 |
| BcgI | CGANNNNNNTGC | 2 cut(s) | 260, 294 |
| BciT130I | CCWGG | 2 cut(s) | 1932, 2147 |
| BcnI | CCSGG | 1 cut(s) | 1022 |
| BcoDI | GTCTC | 4 cut(s) | 258, 1777, 1864, 2474 |
| BfaI | CTAG | 4 cut(s) | 338, 2279, 2430, 2592 |
| BfmI | CTRYAG | 3 cut(s) | 1521, 1794, 2007 |
| BfoI | RGCGCY | 1 cut(s) | 1632 |
| BfrI | CTTAAG | 2 cut(s) | 1541, 2216 |
| BfuAI | ACCTGC | 2 cut(s) | 1528, 2805 |
| BglI | GCCNNNNNGGC | 2 cut(s) | 198, 506 |
| BglII | AGATCT | 1 cut(s) | 1582 |
| BisI | GCNGC | 8 cut(s) | 128, 202, 501, 504, 507, 1108, 1400, 2212 |
| BlnI | CCTAGG | 1 cut(s) | 337 |
| BlpI | GCTNAGC | 1 cut(s) | 1940 |
| BlsI | GCNGC | 8 cut(s) | 129, 203, 502, 505, 508, 1109, 1401, 2213 |
| Bme1390I | CCNGG | 3 cut(s) | 1022, 1932, 2147 |
| Bme18I | GGWCC | 8 cut(s) | 140, 241, 307, 383, 691, 1018, 2199, 2802 |
| BmgBI | CACGTC | 2 cut(s) | 546, 2540 |
| BmrFI | CCNGG | 3 cut(s) | 1022, 1932, 2147 |
| BmrI | ACTGGG | 2 cut(s) | 477, 1802 |
| BmsI | GCATC | 8 cut(s) | 268, 1168, 1345, 1874, 1922, 2055, 2220, 2476 |
| BmuI | ACTGGG | 2 cut(s) | 477, 1802 |
| BpiI | GAAGAC | 1 cut(s) | 372 |
| BpmI | CTGGAG | 2 cut(s) | 1083, 2289 |
| Bpu10I | CCTNAGC | 1 cut(s) | 2169 |
| Bpu1102I | GCTNAGC | 1 cut(s) | 1940 |
| BpuMI | CCSGG | 1 cut(s) | 1022 |
| Bsa29I | ATCGAT | 1 cut(s) | 2029 |
| BsaHI | GRCGYC | 1 cut(s) | 149 |
| BsaI | GGTCTC | 1 cut(s) | 1777 |
| BsaJI | CCNNGG | 5 cut(s) | 193, 310, 337, 467, 501 |
| BsaWI | WCCGGW | 1 cut(s) | 1480 |
| Bsc4I | CCNNNNNNNGG | 5 cut(s) | 123, 126, 297, 2262, 2841 |
| Bse118I | RCCGGY | 2 cut(s) | 380, 1480 |
| Bse1I | ACTGG | 5 cut(s) | 374, 483, 1066, 1706, 1808 |
| Bse3DI | GCAATG | 1 cut(s) | 1139 |
| BseBI | CCWGG | 2 cut(s) | 1932, 2147 |
| BseCI | ATCGAT | 1 cut(s) | 2029 |
| BseDI | CCNNGG | 5 cut(s) | 193, 310, 337, 467, 501 |
| BseGI | GGATG | 9 cut(s) | 72, 400, 619, 1049, 1360, 1376, 2155, 2235, 2746 |
| BseLI | CCNNNNNNNGG | 5 cut(s) | 123, 126, 297, 2262, 2841 |
| BseMI | GCAATG | 1 cut(s) | 1139 |
| BseMII | CTCAG | 2 cut(s) | 1931, 2399 |
| BseNI | ACTGG | 5 cut(s) | 374, 483, 1066, 1706, 1808 |
| BseRI | GAGGAG | 4 cut(s) | 679, 935, 959, 1504 |
| BseSI | GKGCMC | 1 cut(s) | 2599 |
| BseXI | GCAGC | 1 cut(s) | 1386 |
| BsgI | GTGCAG | 1 cut(s) | 827 |
| Bsh1236I | CGCG | 1 cut(s) | 503 |
| BshNI | GGYRCC | 2 cut(s) | 1477, 2182 |
| BshTI | ACCGGT | 1 cut(s) | 1480 |
| BshVI | ATCGAT | 1 cut(s) | 2029 |
| BsiSI | CCGG | 4 cut(s) | 162, 381, 1022, 1481 |
| BslFI | GGGAC | 1 cut(s) | 677 |
| BslI | CCNNNNNNNGG | 5 cut(s) | 123, 126, 297, 2262, 2841 |
| BsmAI | GTCTC | 4 cut(s) | 258, 1777, 1864, 2474 |
| BsmBI | CGTCTC | 1 cut(s) | 258 |
| BsmFI | GGGAC | 1 cut(s) | 677 |
| BsmI | GAATGC | 1 cut(s) | 997 |
| Bso31I | GGTCTC | 1 cut(s) | 1777 |
| Bsp1286I | GDGCHC | 2 cut(s) | 169, 2599 |
| Bsp1720I | GCTNAGC | 1 cut(s) | 1940 |
| Bsp19I | CCATGG | 1 cut(s) | 193 |
| BspCNI | CTCAG | 2 cut(s) | 1932, 2398 |
| BspDI | ATCGAT | 1 cut(s) | 2029 |
| BspFNI | CGCG | 1 cut(s) | 503 |
| BspHI | TCATGA | 1 cut(s) | 1338 |
| BspMAI | CTGCAG | 2 cut(s) | 1525, 1798 |
| BspMI | ACCTGC | 2 cut(s) | 1528, 2805 |
| BspPI | GGATC | 7 cut(s) | 863, 1458, 1559, 1572, 2291, 2765, 2778 |
| BspQI | GCTCTTC | 1 cut(s) | 192 |
| BspT107I | GGYRCC | 2 cut(s) | 1477, 2182 |
| BspTI | CTTAAG | 2 cut(s) | 1541, 2216 |
| BspTNI | GGTCTC | 1 cut(s) | 1777 |
| BsrDI | GCAATG | 1 cut(s) | 1139 |
| BsrFI | RCCGGY | 2 cut(s) | 380, 1480 |
| BsrI | ACTGG | 5 cut(s) | 374, 483, 1066, 1706, 1808 |
| BssAI | RCCGGY | 2 cut(s) | 380, 1480 |
| BssECI | CCNNGG | 5 cut(s) | 193, 310, 337, 467, 501 |
| BssNAI | GTATAC | 1 cut(s) | 1297 |
| BssNI | GRCGYC | 1 cut(s) | 149 |
| BssSI | CACGAG | 1 cut(s) | 375 |
| BssT1I | CCWWGG | 2 cut(s) | 193, 337 |
| Bst1107I | GTATAC | 1 cut(s) | 1297 |
| Bst2BI | CACGAG | 1 cut(s) | 375 |
| Bst2UI | CCWGG | 2 cut(s) | 1932, 2147 |
| Bst4CI | ACNGT | 6 cut(s) | 112, 259, 407, 1328, 1954, 2078 |
| Bst6I | CTCTTC | 6 cut(s) | 192, 297, 969, 1116, 1182, 1356 |
| BstACI | GRCGYC | 1 cut(s) | 149 |
| BstAFI | CTTAAG | 2 cut(s) | 1541, 2216 |
| BstAPI | GCANNNNNTGC | 1 cut(s) | 1031 |
| BstC8I | GCNNGC | 5 cut(s) | 105, 125, 1214, 1937, 2625 |
| BstDEI | CTNAG | 4 cut(s) | 1940, 1998, 2169, 2385 |
| BstDSI | CCRYGG | 2 cut(s) | 193, 501 |
| BstF5I | GGATG | 9 cut(s) | 72, 400, 619, 1049, 1360, 1376, 2155, 2235, 2746 |
| BstFNI | CGCG | 1 cut(s) | 503 |
| BstH2I | RGCGCY | 1 cut(s) | 1632 |
| BstHHI | GCGC | 1 cut(s) | 1631 |
| BstMAI | GTCTC | 4 cut(s) | 258, 1777, 1864, 2474 |
| BstNI | CCWGG | 2 cut(s) | 1932, 2147 |
| BstNSI | RCATGY | 3 cut(s) | 1285, 1939, 2627 |
| BstSCI | CCNGG | 3 cut(s) | 1020, 1930, 2145 |
| BstSFI | CTRYAG | 3 cut(s) | 1521, 1794, 2007 |
| BstSLI | GKGCMC | 1 cut(s) | 2599 |
| BstUI | CGCG | 1 cut(s) | 503 |
| BstV1I | GCAGC | 1 cut(s) | 1386 |
| BstV2I | GAAGAC | 1 cut(s) | 372 |
| BstX2I | RGATCY | 5 cut(s) | 1450, 1564, 1582, 2283, 2770 |
| BstXI | CCANNNNNNTGG | 1 cut(s) | 1633 |
| BstYI | RGATCY | 5 cut(s) | 1450, 1564, 1582, 2283, 2770 |
| BstZ17I | GTATAC | 1 cut(s) | 1297 |
| Bsu15I | ATCGAT | 1 cut(s) | 2029 |
| BsuTUI | ATCGAT | 1 cut(s) | 2029 |
| BtgI | CCRYGG | 2 cut(s) | 193, 501 |
| BtgZI | GCGATG | 3 cut(s) | 19, 363, 524 |
| BtrI | CACGTC | 2 cut(s) | 546, 2540 |
| BtsCI | GGATG | 9 cut(s) | 72, 400, 619, 1049, 1360, 1376, 2155, 2235, 2746 |
| BtsI | GCAGTG | 2 cut(s) | 495, 1157 |
| BtsIMutI | CAGTG | 7 cut(s) | 54, 255, 495, 1157, 1333, 1815, 2055 |
| BveI | ACCTGC | 2 cut(s) | 1528, 2805 |
| Cac8I | GCNNGC | 5 cut(s) | 105, 125, 1214, 1937, 2625 |
| CaiI | CAGNNNCTG | 1 cut(s) | 56 |
| CciI | TCATGA | 1 cut(s) | 1338 |
| CfoI | GCGC | 1 cut(s) | 1631 |
| Cfr10I | RCCGGY | 2 cut(s) | 380, 1480 |
| Cfr42I | CCGCGG | 1 cut(s) | 504 |
| ClaI | ATCGAT | 1 cut(s) | 2029 |
| CpoI | CGGWCCG | 1 cut(s) | 383 |
| CseI | GACGC | 1 cut(s) | 138 |
| Csp6I | GTAC | 3 cut(s) | 274, 1392, 1538 |
| CspAI | ACCGGT | 1 cut(s) | 1480 |
| CspI | CGGWCCG | 1 cut(s) | 383 |
| CviQI | GTAC | 3 cut(s) | 274, 1392, 1538 |
| DdeI | CTNAG | 4 cut(s) | 1940, 1998, 2169, 2385 |
| DraI | TTTAAA | 1 cut(s) | 1422 |
| DrdI | GACNNNNNNGTC | 2 cut(s) | 1769, 2150 |
| DseDI | GACNNNNNNGTC | 2 cut(s) | 1769, 2150 |
| EaeI | YGGCCR | 2 cut(s) | 114, 199 |
| Eam1104I | CTCTTC | 6 cut(s) | 192, 297, 969, 1116, 1182, 1356 |
| EarI | CTCTTC | 6 cut(s) | 192, 297, 969, 1116, 1182, 1356 |
| EciI | GGCGGA | 2 cut(s) | 107, 145 |
| Eco130I | CCWWGG | 2 cut(s) | 193, 337 |
| Eco24I | GRGCYC | 1 cut(s) | 169 |
| Eco31I | GGTCTC | 1 cut(s) | 1777 |
| Eco47I | GGWCC | 8 cut(s) | 140, 241, 307, 383, 691, 1018, 2199, 2802 |
| Eco47III | AGCGCT | 1 cut(s) | 1630 |
| Eco57I | CTGAAG | 2 cut(s) | 231, 2693 |
| EcoO109I | RGGNCCY | 3 cut(s) | 1018, 1667, 2802 |
| EcoRI | GAATTC | 2 cut(s) | 1385, 2017 |
| EcoRII | CCWGG | 2 cut(s) | 1930, 2145 |
| EcoT14I | CCWWGG | 2 cut(s) | 193, 337 |
| EcoT22I | ATGCAT | 2 cut(s) | 1501, 2491 |
| EcoT38I | GRGCYC | 1 cut(s) | 169 |
| ErhI | CCWWGG | 2 cut(s) | 193, 337 |
| Esp3I | CGTCTC | 1 cut(s) | 258 |
| FaqI | GGGAC | 1 cut(s) | 677 |
| FauI | CCCGC | 1 cut(s) | 1033 |
| FblI | GTMKAC | 2 cut(s) | 324, 1296 |
| Fnu4HI | GCNGC | 8 cut(s) | 128, 202, 501, 504, 507, 1108, 1400, 2212 |
| FokI | GGATG | 9 cut(s) | 59, 407, 626, 1056, 1363, 1367, 2162, 2242, 2753 |
| FriOI | GRGCYC | 1 cut(s) | 169 |
| Fsp4HI | GCNGC | 8 cut(s) | 128, 202, 501, 504, 507, 1108, 1400, 2212 |
| FspBI | CTAG | 4 cut(s) | 338, 2279, 2430, 2592 |
| GlaI | GCGC | 1 cut(s) | 1630 |
| GluI | GCNGC | 8 cut(s) | 128, 202, 501, 504, 507, 1108, 1400, 2212 |
| GsuI | CTGGAG | 2 cut(s) | 1083, 2289 |
| HaeII | RGCGCY | 1 cut(s) | 1632 |
| HapII | CCGG | 4 cut(s) | 162, 381, 1022, 1481 |
| HgaI | GACGC | 1 cut(s) | 138 |
| HhaI | GCGC | 1 cut(s) | 1631 |
| Hin1I | GRCGYC | 1 cut(s) | 149 |
| Hin6I | GCGC | 1 cut(s) | 1629 |
| HinP1I | GCGC | 1 cut(s) | 1629 |
| HincII | GTYRAC | 1 cut(s) | 325 |
| HindII | GTYRAC | 1 cut(s) | 325 |
| HindIII | AAGCTT | 2 cut(s) | 1214, 1343 |
| HinfI | GANTC | 8 cut(s) | 133, 246, 373, 398, 788, 1364, 2383, 2662 |
| HpaII | CCGG | 4 cut(s) | 162, 381, 1022, 1481 |
| HphI | GGTGA | 4 cut(s) | 878, 1066, 1079, 2489 |
| Hpy166II | GTNNAC | 6 cut(s) | 325, 543, 1157, 1297, 1923, 1964 |
| Hpy188III | TCNNGA | 8 cut(s) | 232, 694, 1339, 1380, 1586, 2268, 2440, 2654 |
| Hpy8I | GTNNAC | 6 cut(s) | 325, 543, 1157, 1297, 1923, 1964 |
| HpyCH4III | ACNGT | 6 cut(s) | 112, 259, 407, 1328, 1954, 2078 |
| HpyCH4IV | ACGT | 3 cut(s) | 545, 753, 2539 |
| HpyF3I | CTNAG | 4 cut(s) | 1940, 1998, 2169, 2385 |
| HpySE526I | ACGT | 3 cut(s) | 545, 753, 2539 |
| Hsp92I | GRCGYC | 1 cut(s) | 149 |
| HspAI | GCGC | 1 cut(s) | 1629 |
| KspI | CCGCGG | 1 cut(s) | 504 |
| LguI | GCTCTTC | 1 cut(s) | 192 |
| LmnI | GCTCC | 7 cut(s) | 164, 1491, 1636, 1807, 2258, 2270, 2831 |
| Lsp1109I | GCAGC | 1 cut(s) | 1386 |
| LweI | GCATC | 8 cut(s) | 268, 1168, 1345, 1874, 1922, 2055, 2220, 2476 |
| MaeI | CTAG | 4 cut(s) | 338, 2279, 2430, 2592 |
| MaeII | ACGT | 3 cut(s) | 545, 753, 2539 |
| MaeIII | GTNAC | 4 cut(s) | 341, 1816, 1880, 2336 |
| MfeI | CAATTG | 2 cut(s) | 876, 915 |
| MflI | RGATCY | 5 cut(s) | 1450, 1564, 1582, 2283, 2770 |
| MhlI | GDGCHC | 2 cut(s) | 169, 2599 |
| MlsI | TGGCCA | 1 cut(s) | 116 |
| MluNI | TGGCCA | 1 cut(s) | 116 |
| MlyI | GAGTC | 4 cut(s) | 142, 367, 1373, 2377 |
| MmeI | TCCRAC | 4 cut(s) | 241, 1428, 2082, 2101 |
| Mox20I | TGGCCA | 1 cut(s) | 116 |
| Mph1103I | ATGCAT | 2 cut(s) | 1501, 2491 |
| MscI | TGGCCA | 1 cut(s) | 116 |
| MslI | CAYNNNNRTG | 5 cut(s) | 1329, 1904, 1918, 2326, 2520 |
| Msp20I | TGGCCA | 1 cut(s) | 116 |
| MspA1I | CMGCKG | 3 cut(s) | 503, 2389, 2545 |
| MspCI | CTTAAG | 2 cut(s) | 1541, 2216 |
| MspI | CCGG | 4 cut(s) | 162, 381, 1022, 1481 |
| MspR9I | CCNGG | 3 cut(s) | 1022, 1932, 2147 |
| MunI | CAATTG | 2 cut(s) | 876, 915 |
| Mva1269I | GAATGC | 1 cut(s) | 997 |
| MvaI | CCWGG | 2 cut(s) | 1932, 2147 |
| MvnI | CGCG | 1 cut(s) | 503 |
| NciI | CCSGG | 1 cut(s) | 1022 |
| NcoI | CCATGG | 1 cut(s) | 193 |
| NmeAIII | GCCGAG | 1 cut(s) | 448 |
| NmuCI | GTSAC | 2 cut(s) | 1880, 2336 |
| NsiI | ATGCAT | 2 cut(s) | 1501, 2491 |
| NspI | RCATGY | 3 cut(s) | 1285, 1939, 2627 |
| OliI | CACNNNNGTG | 1 cut(s) | 1904 |
| PacI | TTAATTAA | 1 cut(s) | 15 |
| PaeI | GCATGC | 2 cut(s) | 1939, 2627 |
| PagI | TCATGA | 1 cut(s) | 1338 |
| PaqCI | CACCTGC | 1 cut(s) | 2805 |
| PciSI | GCTCTTC | 1 cut(s) | 192 |
| PcsI | WCGNNNNNNNCGW | 5 cut(s) | 267, 273, 282, 542, 1922 |
| PctI | GAATGC | 1 cut(s) | 997 |
| PfeI | GAWTC | 4 cut(s) | 246, 398, 788, 2662 |
| PflFI | GACNNNGTC | 1 cut(s) | 257 |
| PinAI | ACCGGT | 1 cut(s) | 1480 |
| PkrI | GCNGC | 8 cut(s) | 129, 203, 502, 505, 508, 1109, 1401, 2213 |
| PleI | GAGTC | 4 cut(s) | 141, 367, 1372, 2377 |
| PpsI | GAGTC | 4 cut(s) | 141, 367, 1372, 2377 |
| PpuMI | RGGWCCY | 2 cut(s) | 1018, 2802 |
| PshBI | ATTAAT | 2 cut(s) | 813, 1842 |
| PsiI | TTATAA | 1 cut(s) | 2721 |
| Psp1406I | AACGTT | 1 cut(s) | 753 |
| Psp5II | RGGWCCY | 2 cut(s) | 1018, 2802 |
| Psp6I | CCWGG | 2 cut(s) | 1930, 2145 |
| PspGI | CCWGG | 2 cut(s) | 1930, 2145 |
| PspPPI | RGGWCCY | 2 cut(s) | 1018, 2802 |
| PstI | CTGCAG | 2 cut(s) | 1525, 1798 |
| PstNI | CAGNNNCTG | 1 cut(s) | 56 |
| PsuI | RGATCY | 5 cut(s) | 1450, 1564, 1582, 2283, 2770 |
| PsyI | GACNNNGTC | 1 cut(s) | 257 |
| PvuII | CAGCTG | 2 cut(s) | 2389, 2545 |
| RsaI | GTAC | 3 cut(s) | 275, 1393, 1539 |
| RsaNI | GTAC | 3 cut(s) | 274, 1392, 1538 |
| RseI | CAYNNNNRTG | 5 cut(s) | 1329, 1904, 1918, 2326, 2520 |
| Rsr2I | CGGWCCG | 1 cut(s) | 383 |
| RsrII | CGGWCCG | 1 cut(s) | 383 |
| SacII | CCGCGG | 1 cut(s) | 504 |
| SalI | GTCGAC | 1 cut(s) | 323 |
| SapI | GCTCTTC | 1 cut(s) | 192 |
| SatI | GCNGC | 8 cut(s) | 128, 202, 501, 504, 507, 1108, 1400, 2212 |
| SbfI | CCTGCAGG | 1 cut(s) | 1525 |
| SchI | GAGTC | 4 cut(s) | 142, 367, 1373, 2377 |
| ScrFI | CCNGG | 3 cut(s) | 1022, 1932, 2147 |
| SdaI | CCTGCAGG | 1 cut(s) | 1525 |
| SduI | GDGCHC | 2 cut(s) | 169, 2599 |
| SfaNI | GCATC | 8 cut(s) | 268, 1168, 1345, 1874, 1922, 2055, 2220, 2476 |
| SfcI | CTRYAG | 3 cut(s) | 1521, 1794, 2007 |
| Sfr303I | CCGCGG | 1 cut(s) | 504 |
| SgrBI | CCGCGG | 1 cut(s) | 504 |
| SgrDI | CGTCGACG | 1 cut(s) | 323 |
| SinI | GGWCC | 8 cut(s) | 140, 241, 307, 383, 691, 1018, 2199, 2802 |
| SmiMI | CAYNNNNRTG | 5 cut(s) | 1329, 1904, 1918, 2326, 2520 |
| SmlI | CTYRAG | 2 cut(s) | 1541, 2216 |
| SmoI | CTYRAG | 2 cut(s) | 1541, 2216 |
| SphI | GCATGC | 2 cut(s) | 1939, 2627 |
| Sse8387I | CCTGCAGG | 1 cut(s) | 1525 |
| SspI | AATATT | 1 cut(s) | 1078 |
| SspMI | CTAG | 4 cut(s) | 338, 2279, 2430, 2592 |
| StyD4I | CCNGG | 3 cut(s) | 1020, 1930, 2145 |
| StyI | CCWWGG | 2 cut(s) | 193, 337 |
| TaaI | ACNGT | 6 cut(s) | 112, 259, 407, 1328, 1954, 2078 |
| TaiI | ACGT | 3 cut(s) | 548, 756, 2542 |
| TaqI | TCGA | 8 cut(s) | 136, 171, 324, 435, 536, 1367, 2029, 2049 |
| TaqII | GACCGA | 2 cut(s) | 258, 2415 |
| TatI | WGTACW | 1 cut(s) | 1537 |
| TauI | GCSGC | 7 cut(s) | 130, 204, 503, 506, 509, 1110, 2214 |
| TfiI | GAWTC | 4 cut(s) | 246, 398, 788, 2662 |
| TscAI | CASTG | 7 cut(s) | 61, 262, 502, 1164, 1333, 1815, 2062 |
| TseFI | GTSAC | 2 cut(s) | 1880, 2336 |
| TseI | GCWGC | 1 cut(s) | 1399 |
| Tsp45I | GTSAC | 2 cut(s) | 1880, 2336 |
| TspGWI | ACGGA | 3 cut(s) | 375, 1379, 1949 |
| TspRI | CASTG | 7 cut(s) | 61, 262, 502, 1164, 1333, 1815, 2062 |
| Tth111I | GACNNNGTC | 1 cut(s) | 257 |
| Vha464I | CTTAAG | 2 cut(s) | 1541, 2216 |
| VpaK11BI | GGWCC | 8 cut(s) | 140, 241, 307, 383, 691, 1018, 2199, 2802 |
| VspI | ATTAAT | 2 cut(s) | 813, 1842 |
| XapI | RAATTY | 6 cut(s) | 697, 1385, 2017, 2450, 2683, 2746 |
| XceI | RCATGY | 3 cut(s) | 1285, 1939, 2627 |
| XcmI | CCANNNNNNNNNTGG | 2 cut(s) | 847, 2193 |
| XmaJI | CCTAGG | 1 cut(s) | 337 |
| XmiI | GTMKAC | 2 cut(s) | 324, 1296 |
| XspI | CTAG | 4 cut(s) | 338, 2279, 2430, 2592 |
| Zsp2I | ATGCAT | 2 cut(s) | 1501, 2491 |