RLG00000008280

source UniProtKB

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr2
OG0000695
Physical Location & Seq
Forward (+)
28239477 .. 28240578
1102 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000008280

Sequence Viewer

Length: 627 bp
ATGAGGGTGGAAGCAAAACTATATGGGACAACCATTCCCATTGAGTCCCCTCCGACAGGTCACCTTACCAAGGACCTTGGTTCTAGACAAGGTAGTTGCTCCAACTTGAAGAAGAAGACCACAAAGCAAAAGGCTGAGGAGATTGCTAACCCTATAAAGAAGCGCTTAGAGTTAGTTGATGACATAGATGAGAAAATAGAAGAACAAAATGGCAGGGGGTTGCCGGTGGAAGTGGAAGCTGGTATAGAAGTAGCAGCTGCTATAGAGCATGTTCATGCCGCTCCTACAGAGGTTCGTGTTGCTCCTATTAAGTCTGTTGAAGTTACACAGGAGAAACAACCAAATAAGGCCGCAGGTAAGGGTTCGAAGAAAAGGAAATGGCAAACAGCTGGTGATGAAGATGAGTATATTGACTTGAAGAAGCTCCCAAAAGATTACCCTTCACCTTTGAAGTGCTTGTGGTTGTGGGGGAGAGACGCACTAGCCGATGGGAAGACAATCTCTTTCATGCTAACAGATAAAGTATTTGGGATCAATAGGAAGCAATATCTGTATAAGGGAGACATTCATGCATTGTGTACCATGAGTGAACTATCAGGCGGCGTCATCTGCATGTATATGTGGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Peptidase_C48 Transposase_24

Protein Analysis

209

Amino Acids

23.18

Weight (kDa)

8.53

Isoelectric Point (pI)

52.18

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 344
AccBSI CCGCTC 1 cut(s) 281
AciI CCGC 3 cut(s) 279, 351, 600
AclWI GGATC 1 cut(s) 539
AcyI GRCGYC 1 cut(s) 603
AfaI GTAC 1 cut(s) 580
AfeI AGCGCT 1 cut(s) 164
AfiI CCNNNNNNNGG 2 cut(s) 56, 70
AgsI TTSAA 4 cut(s) 109, 320, 418, 451
AluBI AGCT 4 cut(s) 239, 257, 389, 424
AluI AGCT 4 cut(s) 239, 257, 389, 424
Alw26I GTCTC 2 cut(s) 468, 555
AlwI GGATC 1 cut(s) 539
Aor51HI AGCGCT 1 cut(s) 164
AoxI GGCC 1 cut(s) 348
ApeKI GCWGC 2 cut(s) 254, 257
AspLEI GCGC 1 cut(s) 165
AspS9I GGNCC 1 cut(s) 73
AsuHPI GGTGA 3 cut(s) 53, 404, 435
AsuII TTCGAA 1 cut(s) 365
AvaII GGWCC 1 cut(s) 73
BbsI GAAGAC 2 cut(s) 122, 500
BbvCI CCTCAGC 1 cut(s) 135
BbvI GCAGC 2 cut(s) 244, 266
BccI CCATC 1 cut(s) 482
BcoDI GTCTC 2 cut(s) 468, 555
BfaI CTAG 2 cut(s) 84, 482
BfmI CTRYAG 2 cut(s) 261, 285
BfoI RGCGCY 1 cut(s) 166
BfuAI ACCTGC 1 cut(s) 344
BisI GCNGC 5 cut(s) 255, 258, 279, 351, 601
BlsI GCNGC 5 cut(s) 256, 259, 280, 352, 602
Bme18I GGWCC 1 cut(s) 73
BmgT120I GGNCC 1 cut(s) 73
BpiI GAAGAC 2 cut(s) 122, 500
Bpu10I CCTNAGC 1 cut(s) 135
Bpu14I TTCGAA 1 cut(s) 365
BsaHI GRCGYC 1 cut(s) 603
BsaJI CCNNGG 2 cut(s) 69, 76
Bsc4I CCNNNNNNNGG 2 cut(s) 56, 70
Bse118I RCCGGY 1 cut(s) 223
BseDI CCNNGG 2 cut(s) 69, 76
BseLI CCNNNNNNNGG 2 cut(s) 56, 70
BseMII CTCAG 1 cut(s) 126
BseRI GAGGAG 1 cut(s) 152
BseXI GCAGC 2 cut(s) 244, 266
BshFI GGCC 1 cut(s) 350
BsiSI CCGG 1 cut(s) 224
BslFI GGGAC 2 cut(s) 31, 40
BslI CCNNNNNNNGG 2 cut(s) 56, 70
BsmAI GTCTC 2 cut(s) 468, 555
BsmBI CGTCTC 1 cut(s) 468
BsmFI GGGAC 2 cut(s) 31, 40
BsnI GGCC 1 cut(s) 350
Bsp119I TTCGAA 1 cut(s) 365
Bsp143I GATC 1 cut(s) 531
BspACI CCGC 3 cut(s) 279, 351, 600
BspANI GGCC 1 cut(s) 350
BspCNI CTCAG 1 cut(s) 127
BspMI ACCTGC 1 cut(s) 344
BspPI GGATC 1 cut(s) 539
BspT104I TTCGAA 1 cut(s) 365
BsrBI CCGCTC 1 cut(s) 281
BsrFI RCCGGY 1 cut(s) 223
BssAI RCCGGY 1 cut(s) 223
BssECI CCNNGG 2 cut(s) 69, 76
BssMI GATC 1 cut(s) 531
BssNI GRCGYC 1 cut(s) 603
BssT1I CCWWGG 2 cut(s) 69, 76
BstACI GRCGYC 1 cut(s) 603
BstBI TTCGAA 1 cut(s) 365
BstDEI CTNAG 2 cut(s) 135, 166
BstEII GGTNACC 1 cut(s) 59
BstENI CCTNNNNNAGG 2 cut(s) 54, 68
BstH2I RGCGCY 1 cut(s) 166
BstHHI GCGC 1 cut(s) 165
BstKTI GATC 1 cut(s) 534
BstMAI GTCTC 2 cut(s) 468, 555
BstMBI GATC 1 cut(s) 531
BstMWI GCNNNNNNNGC 1 cut(s) 609
BstNSI RCATGY 2 cut(s) 272, 616
BstPI GGTNACC 1 cut(s) 59
BstSFI CTRYAG 2 cut(s) 261, 285
BstV1I GCAGC 2 cut(s) 244, 266
BstV2I GAAGAC 2 cut(s) 122, 500
BsuRI GGCC 1 cut(s) 350
BveI ACCTGC 1 cut(s) 344
CfoI GCGC 1 cut(s) 165
Cfr10I RCCGGY 1 cut(s) 223
Cfr13I GGNCC 1 cut(s) 73
CseI GACGC 2 cut(s) 485, 592
Csp6I GTAC 1 cut(s) 579
CviAII CATG 6 cut(s) 269, 275, 508, 569, 583, 613
CviJI RGCY 7 cut(s) 134, 239, 257, 350, 389, 424, 485
CviKI_1 RGCY 7 cut(s) 134, 239, 257, 350, 389, 424, 485
CviQI GTAC 1 cut(s) 579
DdeI CTNAG 2 cut(s) 135, 166
DpnI GATC 1 cut(s) 533
DpnII GATC 1 cut(s) 531
Eco130I CCWWGG 2 cut(s) 69, 76
Eco47I GGWCC 1 cut(s) 73
Eco47III AGCGCT 1 cut(s) 164
Eco91I GGTNACC 1 cut(s) 59
EcoNI CCTNNNNNAGG 2 cut(s) 54, 68
EcoO109I RGGNCCY 1 cut(s) 73
EcoO65I GGTNACC 1 cut(s) 59
EcoT14I CCWWGG 2 cut(s) 69, 76
EcoT22I ATGCAT 1 cut(s) 574
ErhI CCWWGG 2 cut(s) 69, 76
Esp3I CGTCTC 1 cut(s) 468
FaeI CATG 6 cut(s) 272, 278, 511, 572, 586, 616
FalI AAGNNNNNCTT 2 cut(s) 149, 181
FaqI GGGAC 2 cut(s) 31, 40
FatI CATG 6 cut(s) 268, 274, 507, 568, 582, 612
Fnu4HI GCNGC 5 cut(s) 255, 258, 279, 351, 601
Fsp4HI GCNGC 5 cut(s) 255, 258, 279, 351, 601
FspBI CTAG 2 cut(s) 84, 482
GlaI GCGC 1 cut(s) 164
GluI GCNGC 5 cut(s) 255, 258, 279, 351, 601
HaeII RGCGCY 1 cut(s) 166
HaeIII GGCC 1 cut(s) 350
HapII CCGG 1 cut(s) 224
HgaI GACGC 2 cut(s) 485, 592
HhaI GCGC 1 cut(s) 165
Hin1I GRCGYC 1 cut(s) 603
Hin1II CATG 6 cut(s) 272, 278, 511, 572, 586, 616
Hin6I GCGC 1 cut(s) 163
HinP1I GCGC 1 cut(s) 163
HinfI GANTC 1 cut(s) 44
HpaII CCGG 1 cut(s) 224
HphI GGTGA 3 cut(s) 53, 404, 435
Hpy166II GTNNAC 2 cut(s) 579, 590
Hpy188I TCNGA 1 cut(s) 54
Hpy188III TCNNGA 1 cut(s) 84
Hpy8I GTNNAC 2 cut(s) 579, 590
HpyAV CCTTC 1 cut(s) 450
HpyCH4V TGCA 2 cut(s) 572, 612
HpyF10VI GCNNNNNNNGC 1 cut(s) 609
HpyF3I CTNAG 2 cut(s) 135, 166
Hsp92I GRCGYC 1 cut(s) 603
Hsp92II CATG 6 cut(s) 272, 278, 511, 572, 586, 616
HspAI GCGC 1 cut(s) 163
Kzo9I GATC 1 cut(s) 531
LmnI GCTCC 4 cut(s) 104, 286, 307, 429
LpnPI CCDG 8 cut(s) 42, 199, 225, 237, 314, 339, 375, 582
Lsp1109I GCAGC 2 cut(s) 244, 266
MaeI CTAG 2 cut(s) 84, 482
MaeIII GTNAC 2 cut(s) 59, 322
MalI GATC 1 cut(s) 533
MbiI CCGCTC 1 cut(s) 281
MboI GATC 1 cut(s) 531
MboII GAAGA 8 cut(s) 121, 124, 127, 212, 379, 410, 430, 505
MlyI GAGTC 1 cut(s) 53
MmeI TCCRAC 2 cut(s) 77, 126
MnlI CCTC 3 cut(s) 60, 130, 283
Mph1103I ATGCAT 1 cut(s) 574
MseI TTAA 1 cut(s) 309
MslI CAYNNNNRTG 3 cut(s) 273, 611, 617
MspA1I CMGCKG 2 cut(s) 257, 389
MspI CCGG 1 cut(s) 224
MwoI GCNNNNNNNGC 1 cut(s) 609
NdeII GATC 1 cut(s) 531
NlaIII CATG 6 cut(s) 272, 278, 511, 572, 586, 616
NmuCI GTSAC 1 cut(s) 59
NsiI ATGCAT 1 cut(s) 574
NspI RCATGY 2 cut(s) 272, 616
NspV TTCGAA 1 cut(s) 365
PcsI WCGNNNNNNNCGW 1 cut(s) 483
PkrI GCNGC 5 cut(s) 256, 259, 280, 352, 602
PleI GAGTC 1 cut(s) 52
PpsI GAGTC 1 cut(s) 52
PpuMI RGGWCCY 1 cut(s) 73
Psp5II RGGWCCY 1 cut(s) 73
PspEI GGTNACC 1 cut(s) 59
PspPI GGNCC 1 cut(s) 73
PspPPI RGGWCCY 1 cut(s) 73
PvuII CAGCTG 2 cut(s) 257, 389
RsaI GTAC 1 cut(s) 580
RsaNI GTAC 1 cut(s) 579
RseI CAYNNNNRTG 3 cut(s) 273, 611, 617
SaqAI TTAA 1 cut(s) 309
SatI GCNGC 5 cut(s) 255, 258, 279, 351, 601
Sau3AI GATC 1 cut(s) 531
Sau96I GGNCC 1 cut(s) 73
SchI GAGTC 1 cut(s) 53
SfcI CTRYAG 2 cut(s) 261, 285
SfuI TTCGAA 1 cut(s) 365
SinI GGWCC 1 cut(s) 73
SmiMI CAYNNNNRTG 3 cut(s) 273, 611, 617
SsiI CCGC 3 cut(s) 279, 351, 600
SspMI CTAG 2 cut(s) 84, 482
StyI CCWWGG 2 cut(s) 69, 76
TaqI TCGA 1 cut(s) 365
TauI GCSGC 3 cut(s) 281, 353, 603
Tru1I TTAA 1 cut(s) 309
Tru9I TTAA 1 cut(s) 309
TseFI GTSAC 1 cut(s) 59
TseI GCWGC 2 cut(s) 254, 257
Tsp45I GTSAC 1 cut(s) 59
TspDTI ATGAA 4 cut(s) 263, 411, 496, 557
VpaK11BI GGWCC 1 cut(s) 73
XagI CCTNNNNNAGG 2 cut(s) 54, 68
XbaI TCTAGA 1 cut(s) 83
XceI RCATGY 2 cut(s) 272, 616
XspI CTAG 2 cut(s) 84, 482
Zsp2I ATGCAT 1 cut(s) 574
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.