Rroxscaffold_2G00155770

source UniProtKB

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0000695
Physical Location & Seq
Forward (+)
92193571 .. 92197185
3615 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00155770.1

Sequence Viewer

Length: 804 bp
ATGGAGACCTTCTTGGAAGCAGGAAAAGTGAAGAGACAAGTGAGTGATGGTGAGGTGACATCTTGTGGCAACGATGATGTATTGACAAAGGCTCTAGGCCGGAAGGACCTTGGTTCTGGACAAGGTAGTTGCTCCACTTTGCGGGAGAAGTGTGCATTACAAAAGATTGAGGACGCTGCTGGTATCTGCGCAAAGAAGCGGTTACACTTAGTGGATGACATTGCGAAGGAAGTGGAAGAAGATAAGGTTGTACGAGTTCCGATGGAAGAACCAGTGCTTGTTCCGGTGGAAGAACCAATTGCTGCGGTAGTTCATGAGAAAGGGTCGATCGACAATATTGTTGCTATTGCAACCATTGTTGAAGTGGACAGTAATTGCGAAAACCGGACACTTCATGGTGTTGCTTTGGAAGAAGGGAATGTGCGAGTGTCGATTCTGCGTCCACTTGATCCTCAAGCTAAGGTTCCATTCCCGGTCAACGATGAGATAGTTCTTGTCAAAGATCTTGTTGGGACTTACATTGCTTGGCCGAGGGACCTCATACTTCAATCCCTTCCCGACACTATGAAGAAACCACCAAATAAGTCCAAGGACAATTTGGCAAATGGGAGGACAATCTCCTTCAATTTGGAACAGAAAGTGTTTGGCATCGACGGGAAGCATTATCTGTTTAAGTCGGACATTCATGCATTGTGTACAATGACTGAACTATCAGGTGGAGTAATCTCAATGTATATATGTTATAGCCACTTAGGCAATGTTAATTCTTATGTAAATGTTGGGATGAATATTGCTGATGAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Peptidase_C48 Transposase_24

Protein Analysis

267

Amino Acids

29.17

Weight (kDa)

5.18

Isoelectric Point (pI)

40.49

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF8039 PF26133 113 - 182 7.2e-08 Domain of unknown function (DUF8039)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 190
AciI CCGC 3 cut(s) 142, 199, 305
AclWI GGATC 1 cut(s) 443
AcoI YGGCCR 1 cut(s) 527
AdeI CACNNNGTG 1 cut(s) 211
AfaI GTAC 2 cut(s) 252, 697
AfiI CCNNNNNNNGG 1 cut(s) 141
AgsI TTSAA 3 cut(s) 362, 548, 625
AluBI AGCT 1 cut(s) 458
AluI AGCT 1 cut(s) 458
Alw26I GTCTC 1 cut(s) 28
AlwI GGATC 1 cut(s) 443
AoxI GGCC 2 cut(s) 97, 527
ApeKI GCWGC 2 cut(s) 176, 302
AspLEI GCGC 1 cut(s) 191
AspS9I GGNCC 2 cut(s) 106, 535
AsuC2I CCSGG 1 cut(s) 473
AsuHPI GGTGA 2 cut(s) 62, 67
AvaII GGWCC 2 cut(s) 106, 535
BbvI GCAGC 2 cut(s) 163, 289
BccI CCATC 2 cut(s) 41, 256
BcnI CCSGG 1 cut(s) 473
BcoDI GTCTC 1 cut(s) 28
BfaI CTAG 1 cut(s) 95
BglI GCCNNNNNGGC 1 cut(s) 753
BglII AGATCT 1 cut(s) 502
BisI GCNGC 2 cut(s) 177, 303
BlsI GCNGC 2 cut(s) 178, 304
Bme1390I CCNGG 1 cut(s) 473
Bme18I GGWCC 2 cut(s) 106, 535
BmgT120I GGNCC 2 cut(s) 106, 535
BmiI GGNNCC 2 cut(s) 465, 536
BmrFI CCNGG 1 cut(s) 473
BmsI GCATC 1 cut(s) 657
Bpu10I CCTNAGC 1 cut(s) 459
BpuEI CTTGAG 1 cut(s) 438
BpuMI CCSGG 1 cut(s) 473
BsaJI CCNNGG 3 cut(s) 109, 530, 588
BsaWI WCCGGW 2 cut(s) 283, 384
Bsc4I CCNNNNNNNGG 1 cut(s) 141
Bse1I ACTGG 1 cut(s) 272
Bse3DI GCAATG 3 cut(s) 219, 519, 763
BseDI CCNNGG 3 cut(s) 109, 530, 588
BseGI GGATG 2 cut(s) 220, 789
BseLI CCNNNNNNNGG 1 cut(s) 141
BseMI GCAATG 3 cut(s) 219, 519, 763
BseNI ACTGG 1 cut(s) 272
BseXI GCAGC 2 cut(s) 163, 289
Bsh1285I CGRYCG 1 cut(s) 330
BshFI GGCC 2 cut(s) 99, 529
BsiEI CGRYCG 1 cut(s) 330
BsiSI CCGG 4 cut(s) 100, 284, 385, 473
BslFI GGGAC 2 cut(s) 526, 548
BslI CCNNNNNNNGG 1 cut(s) 141
BsmAI GTCTC 1 cut(s) 28
BsmFI GGGAC 2 cut(s) 526, 548
BsnI GGCC 2 cut(s) 99, 529
Bsp1407I TGTACA 1 cut(s) 695
Bsp143I GATC 3 cut(s) 327, 448, 502
BspACI CCGC 3 cut(s) 142, 199, 305
BspANI GGCC 2 cut(s) 99, 529
BspHI TCATGA 1 cut(s) 313
BspLI GGNNCC 2 cut(s) 465, 536
BspPI GGATC 1 cut(s) 443
BsrDI GCAATG 3 cut(s) 219, 519, 763
BsrGI TGTACA 1 cut(s) 695
BsrI ACTGG 1 cut(s) 272
BssECI CCNNGG 3 cut(s) 109, 530, 588
BssMI GATC 3 cut(s) 327, 448, 502
BssT1I CCWWGG 2 cut(s) 109, 588
Bst4CI ACNGT 1 cut(s) 371
Bst6I CTCTTC 1 cut(s) 26
BstAUI TGTACA 1 cut(s) 695
BstDEI CTNAG 3 cut(s) 208, 459, 751
BstF5I GGATG 2 cut(s) 220, 789
BstHHI GCGC 1 cut(s) 191
BstKTI GATC 3 cut(s) 330, 451, 505
BstMAI GTCTC 1 cut(s) 28
BstMBI GATC 3 cut(s) 327, 448, 502
BstMCI CGRYCG 1 cut(s) 330
BstMWI GCNNNNNNNGC 1 cut(s) 753
BstSCI CCNGG 1 cut(s) 471
BstV1I GCAGC 2 cut(s) 163, 289
BstX2I RGATCY 1 cut(s) 502
BstYI RGATCY 1 cut(s) 502
BsuRI GGCC 2 cut(s) 99, 529
BtsCI GGATG 2 cut(s) 220, 789
BtsIMutI CAGTG 1 cut(s) 279
CciI TCATGA 1 cut(s) 313
CfoI GCGC 1 cut(s) 191
Cfr13I GGNCC 2 cut(s) 106, 535
CseI GACGC 2 cut(s) 182, 428
Csp6I GTAC 2 cut(s) 251, 696
CviAII CATG 3 cut(s) 314, 395, 686
CviJI RGCY 5 cut(s) 92, 99, 458, 529, 747
CviKI_1 RGCY 5 cut(s) 92, 99, 458, 529, 747
CviQI GTAC 2 cut(s) 251, 696
DdeI CTNAG 3 cut(s) 208, 459, 751
DpnI GATC 3 cut(s) 329, 450, 504
DpnII GATC 3 cut(s) 327, 448, 502
DraIII CACNNNGTG 1 cut(s) 211
EaeI YGGCCR 1 cut(s) 527
Eam1104I CTCTTC 1 cut(s) 26
EarI CTCTTC 1 cut(s) 26
Eco130I CCWWGG 2 cut(s) 109, 588
Eco47I GGWCC 2 cut(s) 106, 535
EcoO109I RGGNCCY 2 cut(s) 106, 535
EcoT14I CCWWGG 2 cut(s) 109, 588
EcoT22I ATGCAT 1 cut(s) 691
ErhI CCWWGG 2 cut(s) 109, 588
FaeI CATG 3 cut(s) 317, 398, 689
FaqI GGGAC 2 cut(s) 526, 548
FatI CATG 3 cut(s) 313, 394, 685
FauI CCCGC 1 cut(s) 135
Fnu4HI GCNGC 2 cut(s) 177, 303
FokI GGATG 2 cut(s) 227, 796
Fsp4HI GCNGC 2 cut(s) 177, 303
FspBI CTAG 1 cut(s) 95
FspI TGCGCA 1 cut(s) 190
GlaI GCGC 1 cut(s) 190
GluI GCNGC 2 cut(s) 177, 303
HaeIII GGCC 2 cut(s) 99, 529
HapII CCGG 4 cut(s) 100, 284, 385, 473
HgaI GACGC 2 cut(s) 182, 428
HhaI GCGC 1 cut(s) 191
Hin1II CATG 3 cut(s) 317, 398, 689
Hin6I GCGC 1 cut(s) 189
HinP1I GCGC 1 cut(s) 189
HincII GTYRAC 1 cut(s) 478
HindII GTYRAC 1 cut(s) 478
HinfI GANTC 1 cut(s) 433
HpaII CCGG 4 cut(s) 100, 284, 385, 473
HphI GGTGA 2 cut(s) 62, 67
Hpy166II GTNNAC 4 cut(s) 367, 443, 478, 696
Hpy188I TCNGA 2 cut(s) 261, 679
Hpy188III TCNNGA 3 cut(s) 117, 314, 557
Hpy8I GTNNAC 4 cut(s) 367, 443, 478, 696
Hpy99I CGWCG 1 cut(s) 656
HpyAV CCTTC 6 cut(s) 19, 97, 220, 407, 563, 631
HpyCH4III ACNGT 1 cut(s) 371
HpyCH4V TGCA 3 cut(s) 155, 350, 689
HpyF10VI GCNNNNNNNGC 1 cut(s) 753
HpyF3I CTNAG 3 cut(s) 208, 459, 751
Hsp92II CATG 3 cut(s) 317, 398, 689
HspAI GCGC 1 cut(s) 189
Kzo9I GATC 3 cut(s) 327, 448, 502
LmnI GCTCC 1 cut(s) 137
LpnPI CCDG 9 cut(s) 6, 102, 113, 165, 285, 297, 398, 486, 699
Lsp1109I GCAGC 2 cut(s) 163, 289
LweI GCATC 1 cut(s) 657
MaeI CTAG 1 cut(s) 95
MaeIII GTNAC 2 cut(s) 55, 201
MalI GATC 3 cut(s) 329, 450, 504
MboI GATC 3 cut(s) 327, 448, 502
MboII GAAGA 7 cut(s) 43, 248, 251, 278, 302, 422, 580
MfeI CAATTG 1 cut(s) 297
MflI RGATCY 1 cut(s) 502
MluCI AATT 5 cut(s) 297, 373, 595, 625, 763
MmeI TCCRAC 1 cut(s) 657
MnlI CCTC 6 cut(s) 46, 163, 462, 525, 548, 603
Mph1103I ATGCAT 1 cut(s) 691
MseI TTAA 2 cut(s) 672, 762
MspI CCGG 4 cut(s) 100, 284, 385, 473
MspR9I CCNGG 1 cut(s) 473
MunI CAATTG 1 cut(s) 297
MwoI GCNNNNNNNGC 1 cut(s) 753
NciI CCSGG 1 cut(s) 473
NdeII GATC 3 cut(s) 327, 448, 502
NlaIII CATG 3 cut(s) 317, 398, 689
NlaIV GGNNCC 2 cut(s) 465, 536
NmeAIII GCCGAG 1 cut(s) 555
NmuCI GTSAC 1 cut(s) 55
NsbI TGCGCA 1 cut(s) 190
NsiI ATGCAT 1 cut(s) 691
PagI TCATGA 1 cut(s) 313
PfeI GAWTC 1 cut(s) 433
PkrI GCNGC 2 cut(s) 178, 304
Ple19I CGATCG 1 cut(s) 330
PpuMI RGGWCCY 2 cut(s) 106, 535
Psp5II RGGWCCY 2 cut(s) 106, 535
PspN4I GGNNCC 2 cut(s) 465, 536
PspPI GGNCC 2 cut(s) 106, 535
PspPPI RGGWCCY 2 cut(s) 106, 535
PsuI RGATCY 1 cut(s) 502
PvuI CGATCG 1 cut(s) 330
RsaI GTAC 2 cut(s) 252, 697
RsaNI GTAC 2 cut(s) 251, 696
SaqAI TTAA 2 cut(s) 672, 762
SatI GCNGC 2 cut(s) 177, 303
Sau3AI GATC 3 cut(s) 327, 448, 502
Sau96I GGNCC 2 cut(s) 106, 535
ScrFI CCNGG 1 cut(s) 473
SetI ASST 9 cut(s) 11, 57, 111, 127, 249, 460, 465, 540, 718
SfaNI GCATC 1 cut(s) 657
SinI GGWCC 2 cut(s) 106, 535
SmlI CTYRAG 1 cut(s) 453
SmoI CTYRAG 1 cut(s) 453
Sse9I AATT 5 cut(s) 297, 373, 595, 625, 763
SsiI CCGC 3 cut(s) 142, 199, 305
SspI AATATT 2 cut(s) 337, 790
SspMI CTAG 1 cut(s) 95
StyD4I CCNGG 1 cut(s) 471
StyI CCWWGG 2 cut(s) 109, 588
TaaI ACNGT 1 cut(s) 371
TaqI TCGA 4 cut(s) 326, 330, 431, 651
TasI AATT 5 cut(s) 297, 373, 595, 625, 763
TatI WGTACW 1 cut(s) 695
TfiI GAWTC 1 cut(s) 433
Tru1I TTAA 2 cut(s) 672, 762
Tru9I TTAA 2 cut(s) 672, 762
TscAI CASTG 1 cut(s) 279
TseFI GTSAC 1 cut(s) 55
TseI GCWGC 2 cut(s) 176, 302
Tsp45I GTSAC 1 cut(s) 55
TspDTI ATGAA 5 cut(s) 302, 383, 581, 674, 800
TspRI CASTG 1 cut(s) 279
VpaK11BI GGWCC 2 cut(s) 106, 535
XcmI CCANNNNNNNNNTGG 2 cut(s) 361, 595
XspI CTAG 1 cut(s) 95
Zsp2I ATGCAT 1 cut(s) 691
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.