Rorug03G0245200

source UniProtKB

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000003
OG0000695
Physical Location & Seq
Forward (+)
22942483 .. 22942803
321 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug03G0245200.1

Sequence Viewer

Length: 321 bp
ATGGGGGAGAGCCACAACAACCTCCACCACCGCACAACAATGTCGTCCTCCGCTGCTTCTCGATTGGGTTTGGGGATAAGGACTGCTTCTTCCTCCACCGCTAGCGAAATCGTGGAGGCTCAAGGCCACATTCTAAGGGCCATCGGCCGGAAGGACCGCCACAGCAAGGTTTGCACTACCAAAGGCCCCAGGGACCGCCGCATCCGGCTCGTCGCCCACACTGCACCGCCATTCAATTCTACGACATGCAGGACCGGCTTGGATACTACCGGCCTAGCAAGGCCGTCGATTATCAAGAAAGCCAAGGCCGCAATCAACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Peptidase_C48 Transposase_24

Protein Analysis

106

Amino Acids

11.37

Weight (kDa)

11.55

Isoelectric Point (pI)

46.6

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TCP PF03634 49 - 105 6.8e-12 TCP family transcription factor
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 8 cut(s) 31, 51, 99, 157, 196, 199, 227, 309
AcoI YGGCCR 1 cut(s) 145
AfiI CCNNNNNNNGG 2 cut(s) 147, 166
AgsI TTSAA 1 cut(s) 235
AjnI CCWGG 1 cut(s) 188
AoxI GGCC 7 cut(s) 124, 138, 145, 184, 271, 281, 306
ApeKI GCWGC 1 cut(s) 53
AspS9I GGNCC 5 cut(s) 138, 154, 185, 193, 252
AsuNHI GCTAGC 1 cut(s) 101
AvaII GGWCC 3 cut(s) 154, 193, 252
BbvI GCAGC 1 cut(s) 40
BccI CCATC 1 cut(s) 149
BceAI ACGGC 1 cut(s) 268
BcgI CGANNNNNNTGC 4 cut(s) 190, 224, 267, 301
BciT130I CCWGG 1 cut(s) 190
BciVI GTATCC 1 cut(s) 256
BfaI CTAG 3 cut(s) 102, 275, 319
BfuI GTATCC 1 cut(s) 256
BisI GCNGC 3 cut(s) 54, 199, 309
BlsI GCNGC 3 cut(s) 55, 200, 310
Bme1390I CCNGG 1 cut(s) 190
Bme18I GGWCC 3 cut(s) 154, 193, 252
BmgT120I GGNCC 5 cut(s) 138, 154, 185, 193, 252
BmiI GGNNCC 2 cut(s) 187, 194
BmrFI CCNGG 1 cut(s) 190
BmsI GCATC 1 cut(s) 210
BmtI GCTAGC 1 cut(s) 105
BpuEI CTTGAG 1 cut(s) 105
BsaJI CCNNGG 3 cut(s) 188, 189, 303
Bsc4I CCNNNNNNNGG 2 cut(s) 147, 166
Bse118I RCCGGY 2 cut(s) 254, 269
BseBI CCWGG 1 cut(s) 190
BseDI CCNNGG 3 cut(s) 188, 189, 303
BseGI GGATG 1 cut(s) 201
BseLI CCNNNNNNNGG 2 cut(s) 147, 166
BseX3I CGGCCG 1 cut(s) 145
BseXI GCAGC 1 cut(s) 40
BsgI GTGCAG 1 cut(s) 207
Bsh1285I CGRYCG 1 cut(s) 148
BshFI GGCC 7 cut(s) 126, 140, 147, 186, 273, 283, 308
BsiEI CGRYCG 1 cut(s) 148
BsiSI CCGG 4 cut(s) 148, 205, 255, 270
BslFI GGGAC 1 cut(s) 206
BslI CCNNNNNNNGG 2 cut(s) 147, 166
BsmFI GGGAC 1 cut(s) 206
BsnI GGCC 7 cut(s) 126, 140, 147, 186, 273, 283, 308
BspACI CCGC 8 cut(s) 31, 51, 99, 157, 196, 199, 227, 309
BspANI GGCC 7 cut(s) 126, 140, 147, 186, 273, 283, 308
BspLI GGNNCC 2 cut(s) 187, 194
BspOI GCTAGC 1 cut(s) 105
BsrFI RCCGGY 2 cut(s) 254, 269
BssAI RCCGGY 2 cut(s) 254, 269
BssECI CCNNGG 3 cut(s) 188, 189, 303
BssT1I CCWWGG 1 cut(s) 303
Bst2UI CCWGG 1 cut(s) 190
BstAPI GCANNNNNTGC 1 cut(s) 171
BstC8I GCNNGC 1 cut(s) 103
BstDEI CTNAG 1 cut(s) 134
BstF5I GGATG 1 cut(s) 201
BstMCI CGRYCG 1 cut(s) 148
BstMWI GCNNNNNNNGC 4 cut(s) 171, 221, 255, 308
BstNI CCWGG 1 cut(s) 190
BstNSI RCATGY 1 cut(s) 249
BstSCI CCNGG 1 cut(s) 188
BstV1I GCAGC 1 cut(s) 40
BstZI CGGCCG 1 cut(s) 145
BsuI GTATCC 1 cut(s) 256
BsuRI GGCC 7 cut(s) 126, 140, 147, 186, 273, 283, 308
BtsCI GGATG 1 cut(s) 201
BtsI GCAGTG 1 cut(s) 219
BtsIMutI CAGTG 1 cut(s) 219
Cac8I GCNNGC 1 cut(s) 103
Cfr10I RCCGGY 2 cut(s) 254, 269
Cfr13I GGNCC 5 cut(s) 138, 154, 185, 193, 252
CviAII CATG 1 cut(s) 246
DdeI CTNAG 1 cut(s) 134
EaeI YGGCCR 1 cut(s) 145
EagI CGGCCG 1 cut(s) 145
EclXI CGGCCG 1 cut(s) 145
Eco130I CCWWGG 1 cut(s) 303
Eco47I GGWCC 3 cut(s) 154, 193, 252
Eco52I CGGCCG 1 cut(s) 145
EcoO109I RGGNCCY 1 cut(s) 185
EcoRII CCWGG 1 cut(s) 188
EcoT14I CCWWGG 1 cut(s) 303
ErhI CCWWGG 1 cut(s) 303
FaeI CATG 1 cut(s) 249
FaiI YATR 1 cut(s) 247
FalI AAGNNNNNCTT 2 cut(s) 70, 102
FaqI GGGAC 1 cut(s) 206
FatI CATG 1 cut(s) 245
Fnu4HI GCNGC 3 cut(s) 54, 199, 309
FokI GGATG 1 cut(s) 188
Fsp4HI GCNGC 3 cut(s) 54, 199, 309
FspBI CTAG 3 cut(s) 102, 275, 319
GluI GCNGC 3 cut(s) 54, 199, 309
HaeIII GGCC 7 cut(s) 126, 140, 147, 186, 273, 283, 308
HapII CCGG 4 cut(s) 148, 205, 255, 270
Hin1II CATG 1 cut(s) 249
HpaII CCGG 4 cut(s) 148, 205, 255, 270
Hpy188III TCNNGA 2 cut(s) 60, 295
Hpy99I CGWCG 2 cut(s) 215, 289
HpyAV CCTTC 1 cut(s) 145
HpyCH4V TGCA 3 cut(s) 174, 224, 249
HpyF10VI GCNNNNNNNGC 4 cut(s) 171, 221, 255, 308
HpyF3I CTNAG 1 cut(s) 134
Hsp92II CATG 1 cut(s) 249
LpnPI CCDG 7 cut(s) 161, 175, 202, 218, 235, 268, 283
Lsp1109I GCAGC 1 cut(s) 40
LweI GCATC 1 cut(s) 210
MaeI CTAG 3 cut(s) 102, 275, 319
MboII GAAGA 1 cut(s) 81
MluCI AATT 1 cut(s) 235
MnlI CCTC 4 cut(s) 32, 58, 103, 109
MslI CAYNNNNRTG 1 cut(s) 38
MspA1I CMGCKG 1 cut(s) 53
MspI CCGG 4 cut(s) 148, 205, 255, 270
MspR9I CCNGG 1 cut(s) 190
MvaI CCWGG 1 cut(s) 190
MwoI GCNNNNNNNGC 4 cut(s) 171, 221, 255, 308
NheI GCTAGC 1 cut(s) 101
NlaIII CATG 1 cut(s) 249
NlaIV GGNNCC 2 cut(s) 187, 194
NspI RCATGY 1 cut(s) 249
PasI CCCWGGG 1 cut(s) 189
PkrI GCNGC 3 cut(s) 55, 200, 310
Psp6I CCWGG 1 cut(s) 188
PspGI CCWGG 1 cut(s) 188
PspN4I GGNNCC 2 cut(s) 187, 194
PspPI GGNCC 5 cut(s) 138, 154, 185, 193, 252
RseI CAYNNNNRTG 1 cut(s) 38
SatI GCNGC 3 cut(s) 54, 199, 309
Sau96I GGNCC 5 cut(s) 138, 154, 185, 193, 252
ScrFI CCNGG 1 cut(s) 190
SetI ASST 2 cut(s) 24, 171
SfaNI GCATC 1 cut(s) 210
SinI GGWCC 3 cut(s) 154, 193, 252
SmiMI CAYNNNNRTG 1 cut(s) 38
SmlI CTYRAG 1 cut(s) 120
SmoI CTYRAG 1 cut(s) 120
Sse9I AATT 1 cut(s) 235
SsiI CCGC 8 cut(s) 31, 51, 99, 157, 196, 199, 227, 309
SspMI CTAG 3 cut(s) 102, 275, 319
StyD4I CCNGG 1 cut(s) 188
StyI CCWWGG 1 cut(s) 303
TaqI TCGA 2 cut(s) 61, 287
TasI AATT 1 cut(s) 235
TauI GCSGC 2 cut(s) 201, 311
TscAI CASTG 1 cut(s) 226
TseI GCWGC 1 cut(s) 53
TspRI CASTG 1 cut(s) 226
VpaK11BI GGWCC 3 cut(s) 154, 193, 252
XceI RCATGY 1 cut(s) 249
XspI CTAG 3 cut(s) 102, 275, 319
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.