Rh4CG274400

source UniProtKB

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4C
OG0000695
Physical Location & Seq
Forward (+)
52899549 .. 52908760
9212 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4CG274400.1

Sequence Viewer

Length: 1479 bp
ATGGAAGAGGATGGTGTGCTGAGTGGTGGTGTTGGCATTAAGGTGTTTGTGGAAATGGCACAAGATGTGGAAGTTCTGGGTTTTGATGTAGAAATGGCACAAGATCTGGAAGTTCTAGGATTTGATGCAAAAGGAAAAGCCAATCGAAACATGGTTATGGCTCCAACAAAGACAAGGGCTGCCTCTAGAGCAAGGGCAAATATTCTGAAGGCATTGAGAGTGACAAGAGCGAAGTCTGCGCCTGAAGCAGCAAAACTGGCTCACTCGAATGCTTATGAAAATATGCCTTCCCCAAAGAAAGCAATCACATTGCTGAAGGTCAAAAAGTCATTGACCAAGAAGAAGAAGGGCAAGTCATCAAAGGCCACCTGCCCTGAAAAACATGGCTTACAGTTGTTGAAGCGAGGCTATGTAACTATGCATAGGATTGTTCGTCAAAAAATCCTTGGTGTAAAGCTTAAAGTCTTGTTTAACAAGAAGGGTTCCCCTTATGGTGCTGCAGCGAAGGAAATGCAGTCATACATTGGGTCAGAATCTATCCCTGAGTCAGAACTCGACCGTGCAACAATGTGGATCAAGGCACGGCAATATAAACATGGTAACTTGAAAGATGATGAGGTAGAGCAGAAGGTCGCAGAAATAGACAAGGTGAGGAAGGAAGTGAGTGAAGGATTGGTGACAACCTGTGGCACCGATGATGTGTTGACATTGGCTTTAGGTAACCCAGAGCACCATGGTAGAGTCCTTGGTGTGGGAGGTCATGTCAAGCCTGCTACTTATTTCAACCTGCCTAGGCACCACAGAAAGAGTGTTGAAGAGAGGATTCAGGAAGGCTGTAAGAAATTTATAGAAGAGGAGACAGAGAAAATTGTGGCACGAGAGAGAGCTCATTTTTCCGAGAGGATAATGAGGTTGGAAGCTAAATTACAGGAAGTGAGATCCACTCCCATTGAGTCGCCTCTTGGAGGGCACATTAGCAAGGATGTTGGTTCTGGACAAGGTAGCTGCTCCAATTTGAGGGAGAAGTGTGCAAAGCAGAAGGCTGATGAGGTTGTTGTCACTGCAAAGAAGCGATTGGATTTGGTGGATGACATTGTGAAGGAACTAGAAAAAGAAAGGGTTATAGAGCTGCCGGTGGAGGAACCAGCTGCCGTAGAGGTTCAGGAGAGGGCTATTGAGTTGGTTGAAGTCACACAACAGAAACAACCTGTGAAGGCTGGTAAGGGTTTGAAGAAGAGGAAAAGGGTAGTAGCCATTGATGAAGAGGAGTTATTTATCGACTTCAAGAAGCTGCTGAAAGATTACCCTGCAGCGTGCAAGTGCTTGTGGTTGTGGGCGAAAGACAATTTGGCAAATGGGAAGACAATCTCCTTTCAGATGGAAGACAAAGTTTTTGGCATCAACAAAAAGCAATATGTGTTTAAGGCAGATATTCATGCCTTGTGTACCATGGGTGAATTGTCGGGTGGAGTGATCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Peptidase_C48 Transposase_24

Protein Analysis

492

Amino Acids

54.8

Weight (kDa)

9.34

Isoelectric Point (pI)

42.67

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 377
Acc36I ACCTGC 2 cut(s) 377, 795
AccB1I GGYRCC 2 cut(s) 689, 795
AclWI GGATC 2 cut(s) 581, 933
AcsI RAATTY 1 cut(s) 842
AcuI CTGAAG 3 cut(s) 227, 264, 335
AfaI GTAC 1 cut(s) 1447
AfiI CCNNNNNNNGG 3 cut(s) 751, 965, 1017
AgsI TTSAA 7 cut(s) 400, 607, 784, 815, 1187, 1231, 1285
AjuI GAANNNNNNNTTGG 2 cut(s) 1331, 1363
AluBI AGCT 7 cut(s) 457, 887, 920, 1005, 1129, 1148, 1291
AluI AGCT 7 cut(s) 457, 887, 920, 1005, 1129, 1148, 1291
Alw21I GWGCWC 2 cut(s) 732, 889
Alw26I GTCTC 1 cut(s) 851
AlwI GGATC 2 cut(s) 581, 933
AoxI GGCC 1 cut(s) 363
ApeKI GCWGC 9 cut(s) 179, 248, 497, 500, 1005, 1129, 1148, 1291, 1310
ApoI RAATTY 1 cut(s) 842
ArsI GACNNNNNNTTYG 2 cut(s) 1376, 1408
AspA2I CCTAGG 1 cut(s) 791
AspLEI GCGC 1 cut(s) 241
AsuHPI GGTGA 3 cut(s) 661, 688, 1466
AvrII CCTAGG 1 cut(s) 791
BaeGI GKGCMC 1 cut(s) 972
BanI GGYRCC 2 cut(s) 689, 795
BanII GRGCYC 1 cut(s) 889
BarI GAAGNNNNNNTAC 2 cut(s) 909, 941
BauI CACGAG 1 cut(s) 876
BbsI GAAGAC 2 cut(s) 1367, 1389
Bbv12I GWGCWC 2 cut(s) 732, 889
BbvI GCAGC 9 cut(s) 166, 260, 484, 512, 992, 1116, 1135, 1278, 1322
BccI CCATC 2 cut(s) 5, 1372
BceAI ACGGC 2 cut(s) 599, 1136
BcgI CGANNNNNNTGC 2 cut(s) 493, 527
BcoDI GTCTC 1 cut(s) 851
BfaI CTAG 5 cut(s) 116, 186, 792, 1106, 1477
BfmI CTRYAG 2 cut(s) 498, 1308
BfuAI ACCTGC 2 cut(s) 377, 795
BglII AGATCT 1 cut(s) 103
BisI GCNGC 9 cut(s) 180, 249, 498, 501, 1006, 1130, 1149, 1292, 1311
BlnI CCTAGG 1 cut(s) 791
BlsI GCNGC 9 cut(s) 181, 250, 499, 502, 1007, 1131, 1150, 1293, 1312
BmiI GGNNCC 5 cut(s) 162, 484, 691, 797, 1143
BmsI GCATC 2 cut(s) 115, 1407
BpiI GAAGAC 2 cut(s) 1367, 1389
BplI GAGNNNNNCTC 2 cut(s) 928, 960
BsaJI CCNNGG 5 cut(s) 445, 733, 745, 791, 1449
BsaXI ACNNNNNCTCC 2 cut(s) 747, 777
Bsc4I CCNNNNNNNGG 3 cut(s) 751, 965, 1017
Bse118I RCCGGY 1 cut(s) 1132
Bse1I ACTGG 1 cut(s) 261
Bse3DI GCAATG 1 cut(s) 308
BseDI CCNNGG 5 cut(s) 445, 733, 745, 791, 1449
BseGI GGATG 3 cut(s) 16, 988, 1093
BseLI CCNNNNNNNGG 3 cut(s) 751, 965, 1017
BseMI GCAATG 1 cut(s) 308
BseMII CTCAG 2 cut(s) 11, 534
BseNI ACTGG 1 cut(s) 261
BseRI GAGGAG 2 cut(s) 869, 1280
BseSI GKGCMC 1 cut(s) 972
BseXI GCAGC 9 cut(s) 166, 260, 484, 512, 992, 1116, 1135, 1278, 1322
Bsh1285I CGRYCG 1 cut(s) 559
BshFI GGCC 1 cut(s) 365
BshNI GGYRCC 2 cut(s) 689, 795
BsiEI CGRYCG 1 cut(s) 559
BsiHKAI GWGCWC 2 cut(s) 732, 889
BsiSI CCGG 1 cut(s) 1133
BslI CCNNNNNNNGG 3 cut(s) 751, 965, 1017
BsmAI GTCTC 1 cut(s) 851
BsmI GAATGC 1 cut(s) 274
BsnI GGCC 1 cut(s) 365
Bsp1286I GDGCHC 3 cut(s) 732, 889, 972
Bsp143I GATC 4 cut(s) 103, 573, 938, 1473
Bsp19I CCATGG 2 cut(s) 733, 1449
BspANI GGCC 1 cut(s) 365
BspCNI CTCAG 2 cut(s) 12, 535
BspLI GGNNCC 5 cut(s) 162, 484, 691, 797, 1143
BspMAI CTGCAG 2 cut(s) 502, 1312
BspMI ACCTGC 2 cut(s) 377, 795
BspPI GGATC 2 cut(s) 581, 933
BspT107I GGYRCC 2 cut(s) 689, 795
BsrDI GCAATG 1 cut(s) 308
BsrFI RCCGGY 1 cut(s) 1132
BsrI ACTGG 1 cut(s) 261
BssAI RCCGGY 1 cut(s) 1132
BssECI CCNNGG 5 cut(s) 445, 733, 745, 791, 1449
BssMI GATC 4 cut(s) 103, 573, 938, 1473
BssSI CACGAG 1 cut(s) 876
BssT1I CCWWGG 5 cut(s) 445, 733, 745, 791, 1449
Bst2BI CACGAG 1 cut(s) 876
Bst4CI ACNGT 2 cut(s) 393, 560
Bst6I CTCTTC 4 cut(s) 810, 846, 1229, 1257
BstC8I GCNNGC 2 cut(s) 771, 1315
BstDEI CTNAG 2 cut(s) 20, 543
BstDSI CCRYGG 2 cut(s) 733, 1449
BstEII GGTNACC 1 cut(s) 719
BstENI CCTNNNNNAGG 1 cut(s) 963
BstF5I GGATG 3 cut(s) 16, 988, 1093
BstHHI GCGC 1 cut(s) 241
BstKTI GATC 4 cut(s) 106, 576, 941, 1476
BstMAI GTCTC 1 cut(s) 851
BstMBI GATC 4 cut(s) 103, 573, 938, 1473
BstMCI CGRYCG 1 cut(s) 559
BstMWI GCNNNNNNNGC 4 cut(s) 188, 236, 245, 257
BstPI GGTNACC 1 cut(s) 719
BstSFI CTRYAG 2 cut(s) 498, 1308
BstSLI GKGCMC 1 cut(s) 972
BstV1I GCAGC 9 cut(s) 166, 260, 484, 512, 992, 1116, 1135, 1278, 1322
BstV2I GAAGAC 2 cut(s) 1367, 1389
BstX2I RGATCY 2 cut(s) 103, 938
BstYI RGATCY 2 cut(s) 103, 938
BsuRI GGCC 1 cut(s) 365
BtgI CCRYGG 2 cut(s) 733, 1449
BtsCI GGATG 3 cut(s) 16, 988, 1093
BtsI GCAGTG 1 cut(s) 1059
BtsIMutI CAGTG 1 cut(s) 1059
BveI ACCTGC 2 cut(s) 377, 795
Cac8I GCNNGC 2 cut(s) 771, 1315
CfoI GCGC 1 cut(s) 241
Cfr10I RCCGGY 1 cut(s) 1132
Csp6I GTAC 1 cut(s) 1446
CspCI CAANNNNNGTGG 2 cut(s) 931, 966
CviAII CATG 7 cut(s) 151, 383, 596, 734, 761, 1436, 1450
CviQI GTAC 1 cut(s) 1446
DdeI CTNAG 2 cut(s) 20, 543
DpnI GATC 4 cut(s) 105, 575, 940, 1475
DpnII GATC 4 cut(s) 103, 573, 938, 1473
Eam1104I CTCTTC 4 cut(s) 810, 846, 1229, 1257
EarI CTCTTC 4 cut(s) 810, 846, 1229, 1257
Ecl136II GAGCTC 1 cut(s) 887
Eco130I CCWWGG 5 cut(s) 445, 733, 745, 791, 1449
Eco24I GRGCYC 1 cut(s) 889
Eco53kI GAGCTC 1 cut(s) 887
Eco57I CTGAAG 3 cut(s) 227, 264, 335
Eco91I GGTNACC 1 cut(s) 719
EcoICRI GAGCTC 1 cut(s) 887
EcoNI CCTNNNNNAGG 1 cut(s) 963
EcoO65I GGTNACC 1 cut(s) 719
EcoT14I CCWWGG 5 cut(s) 445, 733, 745, 791, 1449
EcoT22I ATGCAT 1 cut(s) 423
EcoT38I GRGCYC 1 cut(s) 889
ErhI CCWWGG 5 cut(s) 445, 733, 745, 791, 1449
FaeI CATG 7 cut(s) 154, 386, 599, 737, 764, 1439, 1453
FatI CATG 7 cut(s) 150, 382, 595, 733, 760, 1435, 1449
Fnu4HI GCNGC 9 cut(s) 180, 249, 498, 501, 1006, 1130, 1149, 1292, 1311
FokI GGATG 3 cut(s) 23, 995, 1100
FriOI GRGCYC 1 cut(s) 889
Fsp4HI GCNGC 9 cut(s) 180, 249, 498, 501, 1006, 1130, 1149, 1292, 1311
FspBI CTAG 5 cut(s) 116, 186, 792, 1106, 1477
GlaI GCGC 1 cut(s) 240
GluI GCNGC 9 cut(s) 180, 249, 498, 501, 1006, 1130, 1149, 1292, 1311
HaeIII GGCC 1 cut(s) 365
HapII CCGG 1 cut(s) 1133
HhaI GCGC 1 cut(s) 241
Hin1II CATG 7 cut(s) 154, 386, 599, 737, 764, 1439, 1453
Hin6I GCGC 1 cut(s) 239
HinP1I GCGC 1 cut(s) 239
HincII GTYRAC 1 cut(s) 705
HindII GTYRAC 1 cut(s) 705
HindIII AAGCTT 1 cut(s) 455
HinfI GANTC 5 cut(s) 533, 545, 741, 823, 953
HpaII CCGG 1 cut(s) 1133
HphI GGTGA 3 cut(s) 661, 688, 1466
Hpy166II GTNNAC 2 cut(s) 705, 1446
Hpy188I TCNGA 5 cut(s) 207, 532, 550, 898, 1377
Hpy188III TCNNGA 6 cut(s) 107, 186, 827, 993, 1163, 1285
Hpy8I GTNNAC 2 cut(s) 705, 1446
HpyCH4III ACNGT 2 cut(s) 393, 560
HpyCH4V TGCA 9 cut(s) 128, 421, 500, 514, 563, 1031, 1064, 1310, 1317
HpyF10VI GCNNNNNNNGC 4 cut(s) 188, 236, 245, 257
HpyF3I CTNAG 2 cut(s) 20, 543
Hsp92II CATG 7 cut(s) 154, 386, 599, 737, 764, 1439, 1453
HspAI GCGC 1 cut(s) 239
Kzo9I GATC 4 cut(s) 103, 573, 938, 1473
LmnI GCTCC 2 cut(s) 166, 1013
Lsp1109I GCAGC 9 cut(s) 166, 260, 484, 512, 992, 1116, 1135, 1278, 1322
LweI GCATC 2 cut(s) 115, 1407
MaeI CTAG 5 cut(s) 116, 186, 792, 1106, 1477
MaeIII GTNAC 7 cut(s) 220, 412, 599, 676, 719, 1057, 1189
MalI GATC 4 cut(s) 105, 575, 940, 1475
MboI GATC 4 cut(s) 103, 573, 938, 1473
MflI RGATCY 2 cut(s) 103, 938
MhlI GDGCHC 3 cut(s) 732, 889, 972
MluCI AATT 6 cut(s) 842, 867, 923, 1012, 1345, 1457
MlyI GAGTC 3 cut(s) 554, 750, 962
MmeI TCCRAC 2 cut(s) 188, 894
Mph1103I ATGCAT 1 cut(s) 423
MseI TTAA 4 cut(s) 39, 459, 471, 1422
MslI CAYNNNNRTG 3 cut(s) 41, 155, 267
MspA1I CMGCKG 1 cut(s) 1148
MspI CCGG 1 cut(s) 1133
Mva1269I GAATGC 1 cut(s) 274
MwoI GCNNNNNNNGC 4 cut(s) 188, 236, 245, 257
NcoI CCATGG 2 cut(s) 733, 1449
NdeII GATC 4 cut(s) 103, 573, 938, 1473
NlaIII CATG 7 cut(s) 154, 386, 599, 737, 764, 1439, 1453
NlaIV GGNNCC 5 cut(s) 162, 484, 691, 797, 1143
NmuCI GTSAC 4 cut(s) 220, 676, 1057, 1189
NsiI ATGCAT 1 cut(s) 423
PaqCI CACCTGC 1 cut(s) 377
PctI GAATGC 1 cut(s) 274
PfeI GAWTC 2 cut(s) 533, 823
PkrI GCNGC 9 cut(s) 181, 250, 499, 502, 1007, 1131, 1150, 1293, 1312
PleI GAGTC 3 cut(s) 553, 749, 961
PpsI GAGTC 3 cut(s) 553, 749, 961
Psp124BI GAGCTC 1 cut(s) 889
PspEI GGTNACC 1 cut(s) 719
PspN4I GGNNCC 5 cut(s) 162, 484, 691, 797, 1143
PstI CTGCAG 2 cut(s) 502, 1312
PsuI RGATCY 2 cut(s) 103, 938
PvuII CAGCTG 1 cut(s) 1148
RsaI GTAC 1 cut(s) 1447
RsaNI GTAC 1 cut(s) 1446
RseI CAYNNNNRTG 3 cut(s) 41, 155, 267
SacI GAGCTC 1 cut(s) 889
SaqAI TTAA 4 cut(s) 39, 459, 471, 1422
SatI GCNGC 9 cut(s) 180, 249, 498, 501, 1006, 1130, 1149, 1292, 1311
Sau3AI GATC 4 cut(s) 103, 573, 938, 1473
SchI GAGTC 3 cut(s) 554, 750, 962
SduI GDGCHC 3 cut(s) 732, 889, 972
SfaNI GCATC 2 cut(s) 115, 1407
SfcI CTRYAG 2 cut(s) 498, 1308
SmiMI CAYNNNNRTG 3 cut(s) 41, 155, 267
Sse9I AATT 6 cut(s) 842, 867, 923, 1012, 1345, 1457
SspI AATATT 1 cut(s) 202
SspMI CTAG 5 cut(s) 116, 186, 792, 1106, 1477
SstI GAGCTC 1 cut(s) 889
StyI CCWWGG 5 cut(s) 445, 733, 745, 791, 1449
TaaI ACNGT 2 cut(s) 393, 560
TaqI TCGA 4 cut(s) 145, 266, 555, 1278
TasI AATT 6 cut(s) 842, 867, 923, 1012, 1345, 1457
TfiI GAWTC 2 cut(s) 533, 823
Tru1I TTAA 4 cut(s) 39, 459, 471, 1422
Tru9I TTAA 4 cut(s) 39, 459, 471, 1422
TscAI CASTG 1 cut(s) 1066
TseFI GTSAC 4 cut(s) 220, 676, 1057, 1189
TseI GCWGC 9 cut(s) 179, 248, 497, 500, 1005, 1129, 1148, 1291, 1310
Tsp45I GTSAC 4 cut(s) 220, 676, 1057, 1189
TspDTI ATGAA 3 cut(s) 291, 1275, 1424
TspRI CASTG 1 cut(s) 1066
XagI CCTNNNNNAGG 1 cut(s) 963
XapI RAATTY 1 cut(s) 842
XbaI TCTAGA 1 cut(s) 185
XcmI CCANNNNNNNNNTGG 1 cut(s) 148
XmaJI CCTAGG 1 cut(s) 791
XspI CTAG 5 cut(s) 116, 186, 792, 1106, 1477
Zsp2I ATGCAT 1 cut(s) 423
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.