Rh4AG085300

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4A
OG0000695
Physical Location & Seq
Forward (+)
17818099 .. 17844731
26633 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4AG085300.1

Sequence Viewer

Length: 714 bp
ATGTGGATAAAGGCACGGCAGGATAAACACGGCAACTTCAAAGATGTTGAGGTAGAGAAGTGTGCCGCAGACATAGAAGTGGAAGAAGATAAGGTTGTACAGGTTCCGGTGGAAGAACCAGCTGCTGTAGTAGTTCATGAGAAGGCTATTGAGTTGGTTGAAGTTACACAGCAGAAACAACCAAATAAGTCCAGTAAGGGTCTGAAGAAGAGGAAAATGCAATTAGGCAGTCCTGAAGAGGTGAATATTGACATGAAGAAGCTACCAAAAGATTACCCTGCACCTTTGAAGTGCTTGTGGATTTGGACAAGGGACAATTTGGCAAATGGGAGGACAATCTCCTTCAATTTGGAACAGAAAGTGTTTGGCATCGACAGGAAGCATTATCTGTTTAGGTCAGACATTCATGCATTGTGTACAATGACTGAACTATCAGGTGGTTTCAACCCATGGCCAAACTCGACTCGAACTGGAGACCCGACTGATTCTTTACCATCTGGCCTCAGTTCAGCACTGCACCAGTGGTGTTCGACGCCCGCCAGCTTCCTCTTATCCCTCAAGCGGTGGCGACGTTCATTTTGGAATGTAGAAAGCACTGCAACCCAGAAACCATCTCAGCATCTCGCTCAGGCCGACGCGGTCGGAAATCTCAGCTTCGGCGACCTCCAACAATGGGACCTACTAGGTTTTGGAGATCATTTGACCGTCACCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

237

Amino Acids

26.8

Weight (kDa)

7.05

Isoelectric Point (pI)

52.85

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 638
AciI CCGC 4 cut(s) 66, 537, 562, 638
AcoI YGGCCR 1 cut(s) 452
AcuI CTGAAG 2 cut(s) 224, 255
AcyI GRCGYC 1 cut(s) 533
AfaI GTAC 2 cut(s) 99, 418
AfiI CCNNNNNNNGG 2 cut(s) 561, 673
AgsI TTSAA 5 cut(s) 40, 161, 289, 346, 445
AluBI AGCT 4 cut(s) 122, 262, 543, 654
AluI AGCT 4 cut(s) 122, 262, 543, 654
Alw26I GTCTC 1 cut(s) 468
AlwNI CAGNNNCTG 1 cut(s) 125
AoxI GGCC 3 cut(s) 452, 499, 630
ApeKI GCWGC 1 cut(s) 122
ArsI GACNNNNNNTTYG 2 cut(s) 561, 593
AspS9I GGNCC 1 cut(s) 676
AsuHPI GGTGA 2 cut(s) 253, 700
AvaII GGWCC 1 cut(s) 676
BalI TGGCCA 1 cut(s) 454
BbvI GCAGC 1 cut(s) 109
BccI CCATC 2 cut(s) 502, 619
BceAI ACGGC 2 cut(s) 32, 46
BcoDI GTCTC 1 cut(s) 468
BfaI CTAG 2 cut(s) 683, 712
BfmI CTRYAG 1 cut(s) 126
BisI GCNGC 2 cut(s) 66, 123
BlsI GCNGC 2 cut(s) 67, 124
Bme18I GGWCC 1 cut(s) 676
BmgT120I GGNCC 1 cut(s) 676
BmiI GGNNCC 2 cut(s) 105, 677
BmsI GCATC 2 cut(s) 378, 628
BpmI CTGGAG 1 cut(s) 492
Bpu10I CCTNAGC 1 cut(s) 627
BpuEI CTTGAG 1 cut(s) 542
BsaHI GRCGYC 1 cut(s) 533
BsaI GGTCTC 1 cut(s) 468
BsaJI CCNNGG 1 cut(s) 449
BsaWI WCCGGW 1 cut(s) 106
Bsc4I CCNNNNNNNGG 2 cut(s) 561, 673
Bse1I ACTGG 3 cut(s) 192, 475, 520
BseDI CCNNGG 1 cut(s) 449
BseLI CCNNNNNNNGG 2 cut(s) 561, 673
BseMII CTCAG 4 cut(s) 517, 629, 641, 664
BseNI ACTGG 3 cut(s) 192, 475, 520
BseXI GCAGC 1 cut(s) 109
BsgI GTGCAG 2 cut(s) 264, 500
Bsh1236I CGCG 1 cut(s) 638
Bsh1285I CGRYCG 1 cut(s) 642
BshFI GGCC 3 cut(s) 454, 501, 632
BsiEI CGRYCG 1 cut(s) 642
BsiSI CCGG 1 cut(s) 107
BslFI GGGAC 2 cut(s) 326, 689
BslI CCNNNNNNNGG 2 cut(s) 561, 673
BsmAI GTCTC 1 cut(s) 468
BsmFI GGGAC 2 cut(s) 326, 689
BsnI GGCC 3 cut(s) 454, 501, 632
Bso31I GGTCTC 1 cut(s) 468
Bsp1407I TGTACA 2 cut(s) 97, 416
Bsp143I GATC 1 cut(s) 694
Bsp19I CCATGG 1 cut(s) 449
BspACI CCGC 4 cut(s) 66, 537, 562, 638
BspANI GGCC 3 cut(s) 454, 501, 632
BspCNI CTCAG 4 cut(s) 516, 628, 640, 663
BspFNI CGCG 1 cut(s) 638
BspHI TCATGA 1 cut(s) 136
BspLI GGNNCC 2 cut(s) 105, 677
BspTNI GGTCTC 1 cut(s) 468
BsrGI TGTACA 2 cut(s) 97, 416
BsrI ACTGG 3 cut(s) 192, 475, 520
BssECI CCNNGG 1 cut(s) 449
BssMI GATC 1 cut(s) 694
BssNI GRCGYC 1 cut(s) 533
BssT1I CCWWGG 1 cut(s) 449
Bst4CI ACNGT 1 cut(s) 706
Bst6I CTCTTC 2 cut(s) 203, 231
BstACI GRCGYC 1 cut(s) 533
BstAUI TGTACA 2 cut(s) 97, 416
BstC8I GCNNGC 2 cut(s) 537, 541
BstDEI CTNAG 4 cut(s) 503, 615, 627, 650
BstDSI CCRYGG 1 cut(s) 449
BstFNI CGCG 1 cut(s) 638
BstKTI GATC 1 cut(s) 697
BstMAI GTCTC 1 cut(s) 468
BstMBI GATC 1 cut(s) 694
BstMCI CGRYCG 1 cut(s) 642
BstSFI CTRYAG 1 cut(s) 126
BstUI CGCG 1 cut(s) 638
BstV1I GCAGC 1 cut(s) 109
BsuRI GGCC 3 cut(s) 454, 501, 632
BtgI CCRYGG 1 cut(s) 449
BtsI GCAGTG 2 cut(s) 512, 594
BtsIMutI CAGTG 3 cut(s) 512, 527, 594
Cac8I GCNNGC 2 cut(s) 537, 541
CaiI CAGNNNCTG 1 cut(s) 125
CciI TCATGA 1 cut(s) 136
Cfr13I GGNCC 1 cut(s) 676
CseI GACGC 2 cut(s) 541, 644
Csp6I GTAC 2 cut(s) 98, 417
CviAII CATG 4 cut(s) 137, 253, 407, 450
CviJI RGCY 8 cut(s) 122, 146, 262, 454, 501, 543, 632, 654
CviKI_1 RGCY 8 cut(s) 122, 146, 262, 454, 501, 543, 632, 654
CviQI GTAC 2 cut(s) 98, 417
DdeI CTNAG 4 cut(s) 503, 615, 627, 650
DpnI GATC 1 cut(s) 696
DpnII GATC 1 cut(s) 694
EaeI YGGCCR 1 cut(s) 452
Eam1104I CTCTTC 2 cut(s) 203, 231
EarI CTCTTC 2 cut(s) 203, 231
Eco130I CCWWGG 1 cut(s) 449
Eco31I GGTCTC 1 cut(s) 468
Eco47I GGWCC 1 cut(s) 676
Eco57I CTGAAG 2 cut(s) 224, 255
EcoO109I RGGNCCY 1 cut(s) 676
EcoT14I CCWWGG 1 cut(s) 449
EcoT22I ATGCAT 1 cut(s) 412
ErhI CCWWGG 1 cut(s) 449
FaeI CATG 4 cut(s) 140, 256, 410, 453
FaiI YATR 5 cut(s) 74, 138, 254, 408, 451
FaqI GGGAC 2 cut(s) 326, 689
FatI CATG 4 cut(s) 136, 252, 406, 449
FauI CCCGC 1 cut(s) 544
Fnu4HI GCNGC 2 cut(s) 66, 123
Fsp4HI GCNGC 2 cut(s) 66, 123
FspBI CTAG 2 cut(s) 683, 712
GluI GCNGC 2 cut(s) 66, 123
GsuI CTGGAG 1 cut(s) 492
HaeIII GGCC 3 cut(s) 454, 501, 632
HapII CCGG 1 cut(s) 107
HgaI GACGC 2 cut(s) 541, 644
Hin1I GRCGYC 1 cut(s) 533
Hin1II CATG 4 cut(s) 140, 256, 410, 453
HinfI GANTC 2 cut(s) 463, 485
HpaII CCGG 1 cut(s) 107
HphI GGTGA 2 cut(s) 253, 700
Hpy166II GTNNAC 1 cut(s) 417
Hpy188I TCNGA 3 cut(s) 204, 400, 644
Hpy188III TCNNGA 2 cut(s) 137, 233
Hpy8I GTNNAC 1 cut(s) 417
Hpy99I CGWCG 3 cut(s) 535, 573, 638
HpyAV CCTTC 2 cut(s) 136, 352
HpyCH4III ACNGT 1 cut(s) 706
HpyCH4IV ACGT 1 cut(s) 571
HpyCH4V TGCA 5 cut(s) 220, 281, 410, 517, 599
HpyF3I CTNAG 4 cut(s) 503, 615, 627, 650
HpySE526I ACGT 1 cut(s) 571
Hsp92I GRCGYC 1 cut(s) 533
Hsp92II CATG 4 cut(s) 140, 256, 410, 453
Kzo9I GATC 1 cut(s) 694
Lsp1109I GCAGC 1 cut(s) 109
LweI GCATC 2 cut(s) 378, 628
MaeI CTAG 2 cut(s) 683, 712
MaeII ACGT 1 cut(s) 571
MaeIII GTNAC 2 cut(s) 163, 706
MalI GATC 1 cut(s) 696
MboI GATC 1 cut(s) 694
MboII GAAGA 7 cut(s) 95, 98, 125, 217, 220, 248, 268
MlsI TGGCCA 1 cut(s) 454
MluCI AATT 3 cut(s) 221, 316, 346
MluNI TGGCCA 1 cut(s) 454
MlyI GAGTC 1 cut(s) 457
MmeI TCCRAC 2 cut(s) 622, 691
MnlI CCTC 8 cut(s) 43, 204, 232, 324, 512, 557, 566, 674
Mox20I TGGCCA 1 cut(s) 454
Mph1103I ATGCAT 1 cut(s) 412
MscI TGGCCA 1 cut(s) 454
MslI CAYNNNNRTG 1 cut(s) 77
Msp20I TGGCCA 1 cut(s) 454
MspA1I CMGCKG 1 cut(s) 122
MspI CCGG 1 cut(s) 107
MvnI CGCG 1 cut(s) 638
NcoI CCATGG 1 cut(s) 449
NdeII GATC 1 cut(s) 694
NlaIII CATG 4 cut(s) 140, 256, 410, 453
NlaIV GGNNCC 2 cut(s) 105, 677
NmuCI GTSAC 1 cut(s) 706
NsiI ATGCAT 1 cut(s) 412
PagI TCATGA 1 cut(s) 136
PcsI WCGNNNNNNNCGW 1 cut(s) 630
PfeI GAWTC 1 cut(s) 485
PflFI GACNNNGTC 1 cut(s) 638
PkrI GCNGC 2 cut(s) 67, 124
PleI GAGTC 1 cut(s) 457
PpsI GAGTC 1 cut(s) 457
PpuMI RGGWCCY 1 cut(s) 676
Psp5II RGGWCCY 1 cut(s) 676
PspN4I GGNNCC 2 cut(s) 105, 677
PspPI GGNCC 1 cut(s) 676
PspPPI RGGWCCY 1 cut(s) 676
PstNI CAGNNNCTG 1 cut(s) 125
PsyI GACNNNGTC 1 cut(s) 638
PvuII CAGCTG 1 cut(s) 122
RsaI GTAC 2 cut(s) 99, 418
RsaNI GTAC 2 cut(s) 98, 417
RseI CAYNNNNRTG 1 cut(s) 77
SatI GCNGC 2 cut(s) 66, 123
Sau3AI GATC 1 cut(s) 694
Sau96I GGNCC 1 cut(s) 676
SchI GAGTC 1 cut(s) 457
SfaNI GCATC 2 cut(s) 378, 628
SfcI CTRYAG 1 cut(s) 126
SinI GGWCC 1 cut(s) 676
SmiMI CAYNNNNRTG 1 cut(s) 77
SmlI CTYRAG 1 cut(s) 557
SmoI CTYRAG 1 cut(s) 557
Sse9I AATT 3 cut(s) 221, 316, 346
SsiI CCGC 4 cut(s) 66, 537, 562, 638
SspI AATATT 1 cut(s) 247
SspMI CTAG 2 cut(s) 683, 712
StyI CCWWGG 1 cut(s) 449
TaaI ACNGT 1 cut(s) 706
TaiI ACGT 1 cut(s) 574
TaqI TCGA 4 cut(s) 372, 461, 466, 530
TasI AATT 3 cut(s) 221, 316, 346
TatI WGTACW 2 cut(s) 97, 416
TauI GCSGC 1 cut(s) 68
TfiI GAWTC 1 cut(s) 485
TscAI CASTG 3 cut(s) 519, 527, 601
TseFI GTSAC 1 cut(s) 706
TseI GCWGC 1 cut(s) 122
Tsp45I GTSAC 1 cut(s) 706
TspDTI ATGAA 4 cut(s) 125, 269, 395, 564
TspRI CASTG 3 cut(s) 519, 527, 601
Tth111I GACNNNGTC 1 cut(s) 638
VpaK11BI GGWCC 1 cut(s) 676
XspI CTAG 2 cut(s) 683, 712
Zsp2I ATGCAT 1 cut(s) 412
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.