RLG00000020425

source UniProtKB

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0000695
Physical Location & Seq
Forward (+)
64857483 .. 64858551
1069 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000020425

Sequence Viewer

Length: 582 bp
ATGAGATTGGAAGCAAAATTAGATGGGAGAGCCCTTCCCATTGAGTCGCCTCCAACGGGCCATGTTACCAAGGACCTTGGTTCTGGACAATGTAGCTGCTCCAATTTGCGGGAGAAGTGTCCTCTTCAAAAGGCTGAAGACCTTGTTGTCAACGCAAAGAAGCGTTTGGAGTTAGAGGTTGAAGTTCAGGAGAAAGTACAAAAAGAAAATATTGTGAAGCTACCGGTGGAAGAACCAGTGGCTATAGAGGTTGAAGAAAAGGCTATTCAGTTGGTGGAAGTCACACAACAGAAACAACGGCCAAAGTCTGTTAAGGGTCTGAAGAAGAGGAAAAGGCCAGTAGAGGAGGATGAAGAGGAGGATCTCGACTTGAAGAAACTACCAAAAGATTACCCTGCACCGTTGAAATGCTTGTGGTTGTGGGCTAGAGACAATTTGGCAAATGGGAGGACAATGTCCTTTCAGATGGAAGATAAAGTTTTTGGCATCAACAGGAAGCATTATTTGTTTAAGGGAGACATTTATGCATTGTGTACCATGTCTGAATTATCTGGTGGAGTGATCTCCATGTATATTCTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Peptidase_C48 Transposase_24

Protein Analysis

194

Amino Acids

22.0

Weight (kDa)

7.57

Isoelectric Point (pI)

65.62

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 146
AciI CCGC 1 cut(s) 109
AclWI GGATC 1 cut(s) 369
AcoI YGGCCR 1 cut(s) 299
AcuI CTGAAG 2 cut(s) 156, 341
AfaI GTAC 2 cut(s) 198, 535
AfiI CCNNNNNNNGG 2 cut(s) 56, 108
AgeI ACCGGT 1 cut(s) 223
AgsI TTSAA 5 cut(s) 128, 182, 254, 373, 406
AluBI AGCT 2 cut(s) 96, 220
AluI AGCT 2 cut(s) 96, 220
Alw26I GTCTC 2 cut(s) 423, 510
AlwI GGATC 1 cut(s) 369
AoxI GGCC 3 cut(s) 58, 299, 335
ApeKI GCWGC 1 cut(s) 96
AsiGI ACCGGT 1 cut(s) 223
AspS9I GGNCC 2 cut(s) 58, 73
AvaII GGWCC 1 cut(s) 73
BanII GRGCYC 1 cut(s) 34
BbsI GAAGAC 1 cut(s) 144
BbvI GCAGC 1 cut(s) 83
BccI CCATC 2 cut(s) 17, 460
BceAI ACGGC 1 cut(s) 314
BcoDI GTCTC 2 cut(s) 423, 510
BfaI CTAG 1 cut(s) 426
BfmI CTRYAG 1 cut(s) 243
BisI GCNGC 1 cut(s) 97
BlsI GCNGC 1 cut(s) 98
Bme18I GGWCC 1 cut(s) 73
BmgT120I GGNCC 2 cut(s) 58, 73
BmsI GCATC 1 cut(s) 495
BpiI GAAGAC 1 cut(s) 144
BsaJI CCNNGG 2 cut(s) 69, 76
BsaWI WCCGGW 1 cut(s) 223
Bsc4I CCNNNNNNNGG 2 cut(s) 56, 108
Bse118I RCCGGY 1 cut(s) 223
Bse1I ACTGG 2 cut(s) 236, 338
BseDI CCNNGG 2 cut(s) 69, 76
BseGI GGATG 1 cut(s) 355
BseLI CCNNNNNNNGG 2 cut(s) 56, 108
BseNI ACTGG 2 cut(s) 236, 338
BseRI GAGGAG 2 cut(s) 359, 371
BseXI GCAGC 1 cut(s) 83
BsgI GTGCAG 1 cut(s) 381
BshFI GGCC 3 cut(s) 60, 301, 337
BshTI ACCGGT 1 cut(s) 223
BsiSI CCGG 1 cut(s) 224
BslI CCNNNNNNNGG 2 cut(s) 56, 108
BsmAI GTCTC 2 cut(s) 423, 510
BsnI GGCC 3 cut(s) 60, 301, 337
Bsp1286I GDGCHC 1 cut(s) 34
Bsp143I GATC 2 cut(s) 361, 561
BspACI CCGC 1 cut(s) 109
BspANI GGCC 3 cut(s) 60, 301, 337
BspPI GGATC 1 cut(s) 369
BsrFI RCCGGY 1 cut(s) 223
BsrI ACTGG 2 cut(s) 236, 338
BssAI RCCGGY 1 cut(s) 223
BssECI CCNNGG 2 cut(s) 69, 76
BssMI GATC 2 cut(s) 361, 561
BssT1I CCWWGG 2 cut(s) 69, 76
Bst4CI ACNGT 1 cut(s) 402
Bst6I CTCTTC 3 cut(s) 129, 320, 348
BstF5I GGATG 1 cut(s) 355
BstKTI GATC 2 cut(s) 364, 564
BstMAI GTCTC 2 cut(s) 423, 510
BstMBI GATC 2 cut(s) 361, 561
BstSFI CTRYAG 1 cut(s) 243
BstV1I GCAGC 1 cut(s) 83
BstV2I GAAGAC 1 cut(s) 144
BstX2I RGATCY 1 cut(s) 361
BstYI RGATCY 1 cut(s) 361
BsuRI GGCC 3 cut(s) 60, 301, 337
BtsCI GGATG 1 cut(s) 355
BtsIMutI CAGTG 1 cut(s) 243
Cfr10I RCCGGY 1 cut(s) 223
Cfr13I GGNCC 2 cut(s) 58, 73
Csp6I GTAC 2 cut(s) 197, 534
CspAI ACCGGT 1 cut(s) 223
CviAII CATG 3 cut(s) 62, 538, 568
CviQI GTAC 2 cut(s) 197, 534
DpnI GATC 2 cut(s) 363, 563
DpnII GATC 2 cut(s) 361, 561
DrdI GACNNNNNNGTC 1 cut(s) 146
DseDI GACNNNNNNGTC 1 cut(s) 146
EaeI YGGCCR 1 cut(s) 299
Eam1104I CTCTTC 3 cut(s) 129, 320, 348
EarI CTCTTC 3 cut(s) 129, 320, 348
Eco130I CCWWGG 2 cut(s) 69, 76
Eco24I GRGCYC 1 cut(s) 34
Eco47I GGWCC 1 cut(s) 73
Eco57I CTGAAG 2 cut(s) 156, 341
EcoO109I RGGNCCY 1 cut(s) 73
EcoT14I CCWWGG 2 cut(s) 69, 76
EcoT22I ATGCAT 1 cut(s) 529
EcoT38I GRGCYC 1 cut(s) 34
ErhI CCWWGG 2 cut(s) 69, 76
FaeI CATG 3 cut(s) 65, 541, 571
FaiI YATR 6 cut(s) 63, 245, 525, 539, 569, 573
FatI CATG 3 cut(s) 61, 537, 567
FauI CCCGC 1 cut(s) 102
Fnu4HI GCNGC 1 cut(s) 97
FokI GGATG 1 cut(s) 362
FriOI GRGCYC 1 cut(s) 34
Fsp4HI GCNGC 1 cut(s) 97
FspBI CTAG 1 cut(s) 426
GluI GCNGC 1 cut(s) 97
HaeIII GGCC 3 cut(s) 60, 301, 337
HapII CCGG 1 cut(s) 224
Hin1II CATG 3 cut(s) 65, 541, 571
HincII GTYRAC 1 cut(s) 151
HindII GTYRAC 1 cut(s) 151
HinfI GANTC 1 cut(s) 44
HpaII CCGG 1 cut(s) 224
Hpy166II GTNNAC 2 cut(s) 151, 534
Hpy188I TCNGA 3 cut(s) 321, 465, 544
Hpy188III TCNNGA 3 cut(s) 84, 188, 365
Hpy8I GTNNAC 2 cut(s) 151, 534
HpyAV CCTTC 1 cut(s) 44
HpyCH4III ACNGT 1 cut(s) 402
HpyCH4V TGCA 2 cut(s) 398, 527
Hsp92II CATG 3 cut(s) 65, 541, 571
Kzo9I GATC 2 cut(s) 361, 561
LmnI GCTCC 1 cut(s) 104
LpnPI CCDG 8 cut(s) 69, 173, 237, 249, 351, 408, 478, 537
Lsp1109I GCAGC 1 cut(s) 83
LweI GCATC 1 cut(s) 495
MaeI CTAG 1 cut(s) 426
MaeIII GTNAC 2 cut(s) 64, 280
MalI GATC 2 cut(s) 363, 563
MboI GATC 2 cut(s) 361, 561
MboII GAAGA 9 cut(s) 116, 149, 242, 266, 334, 337, 365, 385, 482
MflI RGATCY 1 cut(s) 361
MhlI GDGCHC 1 cut(s) 34
MluCI AATT 4 cut(s) 17, 103, 433, 545
MlyI GAGTC 1 cut(s) 53
MmeI TCCRAC 1 cut(s) 77
Mph1103I ATGCAT 1 cut(s) 529
MseI TTAA 2 cut(s) 312, 510
MspI CCGG 1 cut(s) 224
NdeII GATC 2 cut(s) 361, 561
NlaIII CATG 3 cut(s) 65, 541, 571
NmuCI GTSAC 1 cut(s) 280
NsiI ATGCAT 1 cut(s) 529
PflFI GACNNNGTC 1 cut(s) 454
PinAI ACCGGT 1 cut(s) 223
PkrI GCNGC 1 cut(s) 98
PleI GAGTC 1 cut(s) 52
PpsI GAGTC 1 cut(s) 52
PpuMI RGGWCCY 1 cut(s) 73
Psp5II RGGWCCY 1 cut(s) 73
PspPI GGNCC 2 cut(s) 58, 73
PspPPI RGGWCCY 1 cut(s) 73
PsuI RGATCY 1 cut(s) 361
PsyI GACNNNGTC 1 cut(s) 454
RsaI GTAC 2 cut(s) 198, 535
RsaNI GTAC 2 cut(s) 197, 534
SaqAI TTAA 2 cut(s) 312, 510
SatI GCNGC 1 cut(s) 97
Sau3AI GATC 2 cut(s) 361, 561
Sau96I GGNCC 2 cut(s) 58, 73
SchI GAGTC 1 cut(s) 53
SduI GDGCHC 1 cut(s) 34
SetI ASST 6 cut(s) 78, 98, 144, 180, 222, 252
SfaNI GCATC 1 cut(s) 495
SfcI CTRYAG 1 cut(s) 243
SinI GGWCC 1 cut(s) 73
Sse9I AATT 4 cut(s) 17, 103, 433, 545
SsiI CCGC 1 cut(s) 109
SspI AATATT 1 cut(s) 211
SspMI CTAG 1 cut(s) 426
StyI CCWWGG 2 cut(s) 69, 76
TaaI ACNGT 1 cut(s) 402
TaqI TCGA 1 cut(s) 366
TasI AATT 4 cut(s) 17, 103, 433, 545
TatI WGTACW 1 cut(s) 196
Tru1I TTAA 2 cut(s) 312, 510
Tru9I TTAA 2 cut(s) 312, 510
TscAI CASTG 1 cut(s) 243
TseFI GTSAC 1 cut(s) 280
TseI GCWGC 1 cut(s) 96
Tsp45I GTSAC 1 cut(s) 280
TspDTI ATGAA 1 cut(s) 366
TspRI CASTG 1 cut(s) 243
Tth111I GACNNNGTC 1 cut(s) 454
VpaK11BI GGWCC 1 cut(s) 73
XspI CTAG 1 cut(s) 426
Zsp2I ATGCAT 1 cut(s) 529
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.