Rh3BG323300

source UniProtKB

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr3B
OG0000695
Physical Location & Seq
Forward (+)
33441890 .. 33451963
10074 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh3BG323300.1

Sequence Viewer

Length: 471 bp
ATGTCACATGTGAAGAAACAACCAAATAAGGCCGCAGGTAAGGGCTCGAAGAAAAGGAAAAGGCAAACAGCTGGTGATGAAGAGGAGTATATTGACTTGAAGAAGCTGCCAAAAGATTACCCTTCACCTTTGAAGTGCTTGTGGTTGTGGGGGAGAGACGCACTAGCCGATGGGAAGACAATCTCTTTCATGCTAACAGATAAAGTATTTGGGATCAATAGGAAGCAATATCTGTATAAGGGAGACATTCATGCATTGTGTACCATGAGTGAACTATCAGGCGGCGTCATCTGCATGTATATGTGTGGGATCAAAATTTACAATGCTCAGAGGAATCGATCTGGCCGGAAATCAATAACGTGGCACAATATGGGGTGGCAGCGACGATCAGATCTGGTATACACCCAGAAGGATCTTGATGATGTCAGGTCAGAGTGGGCTAGGTTCGTCATGAAGTATCATGCAACATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Peptidase_C48 Transposase_24

Protein Analysis

156

Amino Acids

18.13

Weight (kDa)

9.74

Isoelectric Point (pI)

58.83

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 26
AccI GTMKAC 1 cut(s) 399
AciI CCGC 2 cut(s) 33, 282
AclWI GGATC 3 cut(s) 221, 317, 420
AcoI YGGCCR 1 cut(s) 343
AcsI RAATTY 1 cut(s) 315
AcyI GRCGYC 1 cut(s) 285
AfaI GTAC 1 cut(s) 262
AflIII ACRYGT 1 cut(s) 7
AgsI TTSAA 2 cut(s) 100, 133
AluBI AGCT 2 cut(s) 71, 106
AluI AGCT 2 cut(s) 71, 106
Alw26I GTCTC 2 cut(s) 150, 237
AlwI GGATC 3 cut(s) 221, 317, 420
AoxI GGCC 2 cut(s) 30, 343
ApeKI GCWGC 2 cut(s) 106, 379
ApoI RAATTY 1 cut(s) 315
AsuHPI GGTGA 2 cut(s) 86, 117
BanII GRGCYC 1 cut(s) 47
BbsI GAAGAC 1 cut(s) 182
BbvI GCAGC 2 cut(s) 93, 391
BccI CCATC 1 cut(s) 164
BcoDI GTCTC 2 cut(s) 150, 237
BfaI CTAG 2 cut(s) 164, 441
BfuAI ACCTGC 1 cut(s) 26
BglII AGATCT 1 cut(s) 391
BisI GCNGC 4 cut(s) 33, 107, 283, 380
BlsI GCNGC 4 cut(s) 34, 108, 284, 381
BpiI GAAGAC 1 cut(s) 182
Bsa29I ATCGAT 1 cut(s) 337
BsaHI GRCGYC 1 cut(s) 285
BseCI ATCGAT 1 cut(s) 337
BseMII CTCAG 1 cut(s) 341
BseRI GAGGAG 1 cut(s) 98
BseXI GCAGC 2 cut(s) 93, 391
BshFI GGCC 2 cut(s) 32, 345
BshVI ATCGAT 1 cut(s) 337
BsiSI CCGG 1 cut(s) 346
BsmAI GTCTC 2 cut(s) 150, 237
BsmBI CGTCTC 1 cut(s) 150
BsnI GGCC 2 cut(s) 32, 345
Bsp1286I GDGCHC 1 cut(s) 47
Bsp143I GATC 6 cut(s) 213, 309, 338, 386, 391, 412
BspACI CCGC 2 cut(s) 33, 282
BspANI GGCC 2 cut(s) 32, 345
BspCNI CTCAG 1 cut(s) 340
BspDI ATCGAT 1 cut(s) 337
BspHI TCATGA 1 cut(s) 450
BspMI ACCTGC 1 cut(s) 26
BspPI GGATC 3 cut(s) 221, 317, 420
BssMI GATC 6 cut(s) 213, 309, 338, 386, 391, 412
BssNAI GTATAC 1 cut(s) 400
BssNI GRCGYC 1 cut(s) 285
Bst1107I GTATAC 1 cut(s) 400
Bst6I CTCTTC 1 cut(s) 75
BstACI GRCGYC 1 cut(s) 285
BstDEI CTNAG 1 cut(s) 327
BstKTI GATC 6 cut(s) 216, 312, 341, 389, 394, 415
BstMAI GTCTC 2 cut(s) 150, 237
BstMBI GATC 6 cut(s) 213, 309, 338, 386, 391, 412
BstMWI GCNNNNNNNGC 1 cut(s) 291
BstNSI RCATGY 2 cut(s) 11, 298
BstV1I GCAGC 2 cut(s) 93, 391
BstV2I GAAGAC 1 cut(s) 182
BstX2I RGATCY 2 cut(s) 391, 412
BstYI RGATCY 2 cut(s) 391, 412
BstZ17I GTATAC 1 cut(s) 400
Bsu15I ATCGAT 1 cut(s) 337
BsuRI GGCC 2 cut(s) 32, 345
BsuTUI ATCGAT 1 cut(s) 337
BveI ACCTGC 1 cut(s) 26
CciI TCATGA 1 cut(s) 450
ClaI ATCGAT 1 cut(s) 337
CseI GACGC 2 cut(s) 167, 274
Csp6I GTAC 1 cut(s) 261
CviAII CATG 7 cut(s) 8, 190, 251, 265, 295, 451, 461
CviJI RGCY 7 cut(s) 32, 45, 71, 106, 167, 345, 440
CviKI_1 RGCY 7 cut(s) 32, 45, 71, 106, 167, 345, 440
CviQI GTAC 1 cut(s) 261
DdeI CTNAG 1 cut(s) 327
DpnI GATC 6 cut(s) 215, 311, 340, 388, 393, 414
DpnII GATC 6 cut(s) 213, 309, 338, 386, 391, 412
EaeI YGGCCR 1 cut(s) 343
Eam1104I CTCTTC 1 cut(s) 75
EarI CTCTTC 1 cut(s) 75
Eco24I GRGCYC 1 cut(s) 47
EcoT22I ATGCAT 1 cut(s) 256
EcoT38I GRGCYC 1 cut(s) 47
Esp3I CGTCTC 1 cut(s) 150
FaeI CATG 7 cut(s) 11, 193, 254, 268, 298, 454, 464
FatI CATG 7 cut(s) 7, 189, 250, 264, 294, 450, 460
FblI GTMKAC 1 cut(s) 399
Fnu4HI GCNGC 4 cut(s) 33, 107, 283, 380
FriOI GRGCYC 1 cut(s) 47
Fsp4HI GCNGC 4 cut(s) 33, 107, 283, 380
FspBI CTAG 2 cut(s) 164, 441
GluI GCNGC 4 cut(s) 33, 107, 283, 380
HaeIII GGCC 2 cut(s) 32, 345
HapII CCGG 1 cut(s) 346
HgaI GACGC 2 cut(s) 167, 274
Hin1I GRCGYC 1 cut(s) 285
Hin1II CATG 7 cut(s) 11, 193, 254, 268, 298, 454, 464
HinfI GANTC 1 cut(s) 334
HpaII CCGG 1 cut(s) 346
HphI GGTGA 2 cut(s) 86, 117
Hpy166II GTNNAC 3 cut(s) 261, 272, 400
Hpy188I TCNGA 3 cut(s) 330, 391, 433
Hpy188III TCNNGA 2 cut(s) 416, 451
Hpy8I GTNNAC 3 cut(s) 261, 272, 400
Hpy99I CGWCG 1 cut(s) 387
HpyAV CCTTC 2 cut(s) 132, 403
HpyCH4IV ACGT 1 cut(s) 359
HpyCH4V TGCA 3 cut(s) 254, 294, 464
HpyF10VI GCNNNNNNNGC 1 cut(s) 291
HpyF3I CTNAG 1 cut(s) 327
HpySE526I ACGT 1 cut(s) 359
Hsp92I GRCGYC 1 cut(s) 285
Hsp92II CATG 7 cut(s) 11, 193, 254, 268, 298, 454, 464
Kzo9I GATC 6 cut(s) 213, 309, 338, 386, 391, 412
LpnPI CCDG 8 cut(s) 21, 57, 264, 327, 359, 380, 412, 419
Lsp1109I GCAGC 2 cut(s) 93, 391
MaeI CTAG 2 cut(s) 164, 441
MaeII ACGT 1 cut(s) 359
MaeIII GTNAC 1 cut(s) 3
MalI GATC 6 cut(s) 215, 311, 340, 388, 393, 414
MboI GATC 6 cut(s) 213, 309, 338, 386, 391, 412
MboII GAAGA 5 cut(s) 25, 61, 92, 112, 187
MflI RGATCY 2 cut(s) 391, 412
MhlI GDGCHC 1 cut(s) 47
MluCI AATT 1 cut(s) 315
MnlI CCTC 2 cut(s) 76, 324
Mph1103I ATGCAT 1 cut(s) 256
MslI CAYNNNNRTG 2 cut(s) 293, 299
MspA1I CMGCKG 1 cut(s) 71
MspI CCGG 1 cut(s) 346
MwoI GCNNNNNNNGC 1 cut(s) 291
NdeII GATC 6 cut(s) 213, 309, 338, 386, 391, 412
NlaIII CATG 7 cut(s) 11, 193, 254, 268, 298, 454, 464
NmuCI GTSAC 1 cut(s) 3
NsiI ATGCAT 1 cut(s) 256
NspI RCATGY 2 cut(s) 11, 298
PagI TCATGA 1 cut(s) 450
PciI ACATGT 1 cut(s) 7
PcsI WCGNNNNNNNCGW 1 cut(s) 165
PfeI GAWTC 1 cut(s) 334
PkrI GCNGC 4 cut(s) 34, 108, 284, 381
PscI ACATGT 1 cut(s) 7
PsuI RGATCY 2 cut(s) 391, 412
PvuII CAGCTG 1 cut(s) 71
RsaI GTAC 1 cut(s) 262
RsaNI GTAC 1 cut(s) 261
RseI CAYNNNNRTG 2 cut(s) 293, 299
SatI GCNGC 4 cut(s) 33, 107, 283, 380
Sau3AI GATC 6 cut(s) 213, 309, 338, 386, 391, 412
SduI GDGCHC 1 cut(s) 47
SetI ASST 7 cut(s) 40, 73, 108, 130, 362, 431, 446
SmiMI CAYNNNNRTG 2 cut(s) 293, 299
Sse9I AATT 1 cut(s) 315
SsiI CCGC 2 cut(s) 33, 282
SspMI CTAG 2 cut(s) 164, 441
TaiI ACGT 1 cut(s) 362
TaqI TCGA 2 cut(s) 47, 337
TasI AATT 1 cut(s) 315
TauI GCSGC 2 cut(s) 35, 285
TfiI GAWTC 1 cut(s) 334
TseFI GTSAC 1 cut(s) 3
TseI GCWGC 2 cut(s) 106, 379
Tsp45I GTSAC 1 cut(s) 3
TspDTI ATGAA 4 cut(s) 93, 178, 239, 467
XapI RAATTY 1 cut(s) 315
XceI RCATGY 2 cut(s) 11, 298
XmiI GTMKAC 1 cut(s) 399
XspI CTAG 2 cut(s) 164, 441
Zsp2I ATGCAT 1 cut(s) 256
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.