Rmu_sc0003002.1_g000067

source UniProtKB

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0003002.1
OG0000695
Physical Location & Seq
Forward (+)
282517 .. 285253
2737 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0003002.1_g000067.1.cds

Sequence Viewer

Length: 1344 bp
atgtcacgtaagggatacgcaaatctagaggctgaattgtctgaaatcaatcctgattcggaaatcgaccatgcaacaatgtggatcaaggcacagcaggataaacatggcaacttcaaagatgttgaggaaaagctgaagaggcaagtcagtgatggtgaggtgacatcttgtggcactgatgatgtattgacaaaggctctaggtaaccctgaacaccatggaagagtgcgtggtgtggggggtcatgtcaagcctggcatatattttaacctaccgaggcaaaaaaggaaaattgttgaagagagaattaaggaagactgcaagaaattaatagaggaggaggcagagaaaattgtgacacgagagagagcacattgggctgaaacaataatgaggttggaagcaaaacttgaagggaaaacctttgccattgagtcccctacggcaggccagaaggaccttggttctggacaaggtagttgctccactttgcgggagaagtgtgcattacaaaagattgaggacgctgctggtatctttgcaaagaagcggttagacttagtggatgacagtgtgaaggaagtggaagaagataaggttgtacaggttccgatggaagaaccagctgctgcagtagttcatgagaaggctgttgagttggttgaagttacacagcaagaacttaaagagttcgagtgcagattggctttagggtcgatcgacaatattgttgctattgctaccattgttgaagtggacagtaatggcgaaaaccagacacttcatggtgttgctttggaagaagggaatgtgcgagtgtcgattctgcgtccacttgatcctcaagctaaggttccattcccagtcaacgatgagatagttactgtcaaagatgctgttgggacttacattgcttggccgagggacctcatacttcaatcccctcctgacattatgaagaaacaaccaaataagtccagtaagggtttgaagaagaggaaaaggcaagtaggcattcctgaagaggagaatattgacatgaagaagctaccaaaagattaccctgcacctttgaagtgcttgtggatttgggcaagggacaatttggcaaatgggaggacaatctccttcaatttggaacagaaaggtattccagcacaacaaaatgtgaaggactgtggcatattcatcatgcggtacatgaaggaaataattgaggataagaacctgggttttgcttcaaagtggcagcgacgatccgagttgatctacacccaaaaggatgttgatgttgtgaggtcagaatgggctaagtttgtgatgaagtatcatatttcatag
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Peptidase_C48 Transposase_24

Protein Analysis

447

Amino Acids

50.49

Weight (kDa)

6.23

Isoelectric Point (pI)

46.69

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 3 cut(s) 496, 553, 1198
AclWI GGATC 3 cut(s) 92, 836, 1254
AcoI YGGCCR 1 cut(s) 920
AcuI CTGAAG 2 cut(s) 158, 1044
AfaI GTAC 2 cut(s) 606, 1202
AfiI CCNNNNNNNGG 2 cut(s) 449, 495
AjnI CCWGG 2 cut(s) 256, 1230
AluBI AGCT 4 cut(s) 136, 629, 851, 1051
AluI AGCT 4 cut(s) 136, 629, 851, 1051
Alw21I GWGCWC 1 cut(s) 376
AlwI GGATC 3 cut(s) 92, 836, 1254
AlwNI CAGNNNCTG 1 cut(s) 632
AoxI GGCC 2 cut(s) 451, 920
ApeKI GCWGC 4 cut(s) 530, 629, 632, 1252
AseI ATTAAT 1 cut(s) 332
AspS9I GGNCC 2 cut(s) 460, 928
AsuHPI GGTGA 2 cut(s) 170, 175
AvaII GGWCC 2 cut(s) 460, 928
BauI CACGAG 1 cut(s) 363
BbsI GAAGAC 1 cut(s) 324
Bbv12I GWGCWC 1 cut(s) 376
BbvI GCAGC 4 cut(s) 517, 616, 619, 1264
BccI CCATC 2 cut(s) 149, 610
BceAI ACGGC 1 cut(s) 462
BciT130I CCWGG 2 cut(s) 258, 1232
BciVI GTATCC 1 cut(s) 8
BfaI CTAG 2 cut(s) 26, 203
BfmI CTRYAG 1 cut(s) 633
BfuI GTATCC 1 cut(s) 8
BisI GCNGC 4 cut(s) 531, 630, 633, 1253
BlsI GCNGC 4 cut(s) 532, 631, 634, 1254
Bme1390I CCNGG 2 cut(s) 258, 1232
Bme18I GGWCC 2 cut(s) 460, 928
BmgT120I GGNCC 2 cut(s) 460, 928
BmiI GGNNCC 3 cut(s) 612, 858, 929
BmrFI CCNGG 2 cut(s) 258, 1232
BmrI ACTGGG 1 cut(s) 860
BmsI GCATC 1 cut(s) 886
BmuI ACTGGG 1 cut(s) 860
BpiI GAAGAC 1 cut(s) 324
Bpu10I CCTNAGC 1 cut(s) 852
BpuEI CTTGAG 1 cut(s) 831
BsaAI YACGTR 1 cut(s) 8
BsaJI CCNNGG 5 cut(s) 220, 278, 463, 923, 1231
Bsc4I CCNNNNNNNGG 2 cut(s) 449, 495
Bse1I ACTGG 2 cut(s) 866, 981
Bse3DI GCAATG 1 cut(s) 912
BseBI CCWGG 2 cut(s) 258, 1232
BseDI CCNNGG 5 cut(s) 220, 278, 463, 923, 1231
BseGI GGATG 2 cut(s) 574, 1291
BseLI CCNNNNNNNGG 2 cut(s) 449, 495
BseMI GCAATG 1 cut(s) 912
BseNI ACTGG 2 cut(s) 866, 981
BseRI GAGGAG 3 cut(s) 353, 356, 1043
BseXI GCAGC 4 cut(s) 517, 616, 619, 1264
BsgI GTGCAG 2 cut(s) 721, 1053
Bsh1285I CGRYCG 1 cut(s) 723
BshFI GGCC 2 cut(s) 453, 922
BsiEI CGRYCG 1 cut(s) 723
BsiHKAI GWGCWC 1 cut(s) 376
BslFI GGGAC 4 cut(s) 424, 919, 941, 1115
BslI CCNNNNNNNGG 2 cut(s) 449, 495
BsmFI GGGAC 4 cut(s) 424, 919, 941, 1115
BsmI GAATGC 1 cut(s) 1017
BsnI GGCC 2 cut(s) 453, 922
Bsp1286I GDGCHC 1 cut(s) 376
Bsp1407I TGTACA 1 cut(s) 604
Bsp143I GATC 5 cut(s) 84, 720, 841, 1259, 1269
Bsp19I CCATGG 1 cut(s) 220
BspACI CCGC 3 cut(s) 496, 553, 1198
BspANI GGCC 2 cut(s) 453, 922
BspHI TCATGA 1 cut(s) 643
BspLI GGNNCC 3 cut(s) 612, 858, 929
BspMAI CTGCAG 1 cut(s) 637
BspPI GGATC 3 cut(s) 92, 836, 1254
BsrDI GCAATG 1 cut(s) 912
BsrGI TGTACA 1 cut(s) 604
BsrI ACTGG 2 cut(s) 866, 981
BssECI CCNNGG 5 cut(s) 220, 278, 463, 923, 1231
BssMI GATC 5 cut(s) 84, 720, 841, 1259, 1269
BssSI CACGAG 1 cut(s) 363
BssT1I CCWWGG 2 cut(s) 220, 463
Bst2BI CACGAG 1 cut(s) 363
Bst2UI CCWGG 2 cut(s) 258, 1232
Bst4CI ACNGT 4 cut(s) 575, 764, 889, 1181
Bst6I CTCTTC 5 cut(s) 134, 220, 297, 992, 1020
BstAUI TGTACA 1 cut(s) 604
BstBAI YACGTR 1 cut(s) 8
BstC8I GCNNGC 1 cut(s) 451
BstDEI CTNAG 3 cut(s) 562, 852, 1314
BstDSI CCRYGG 1 cut(s) 220
BstEII GGTNACC 1 cut(s) 206
BstENI CCTNNNNNAGG 1 cut(s) 447
BstF5I GGATG 2 cut(s) 574, 1291
BstKTI GATC 5 cut(s) 87, 723, 844, 1262, 1272
BstMBI GATC 5 cut(s) 84, 720, 841, 1259, 1269
BstMCI CGRYCG 1 cut(s) 723
BstMWI GCNNNNNNNGC 2 cut(s) 142, 380
BstNI CCWGG 2 cut(s) 258, 1232
BstPI GGTNACC 1 cut(s) 206
BstSCI CCNGG 2 cut(s) 256, 1230
BstSFI CTRYAG 1 cut(s) 633
BstV1I GCAGC 4 cut(s) 517, 616, 619, 1264
BstV2I GAAGAC 1 cut(s) 324
BsuI GTATCC 1 cut(s) 8
BsuRI GGCC 2 cut(s) 453, 922
BtgI CCRYGG 1 cut(s) 220
BtsCI GGATG 2 cut(s) 574, 1291
BtsIMutI CAGTG 3 cut(s) 157, 177, 580
Cac8I GCNNGC 1 cut(s) 451
CaiI CAGNNNCTG 1 cut(s) 632
CciI TCATGA 1 cut(s) 643
Cfr13I GGNCC 2 cut(s) 460, 928
CseI GACGC 2 cut(s) 536, 821
Csp6I GTAC 2 cut(s) 605, 1201
CviAII CATG 9 cut(s) 71, 107, 221, 248, 644, 788, 1042, 1195, 1204
CviQI GTAC 2 cut(s) 605, 1201
DdeI CTNAG 3 cut(s) 562, 852, 1314
DpnI GATC 5 cut(s) 86, 722, 843, 1261, 1271
DpnII GATC 5 cut(s) 84, 720, 841, 1259, 1269
EaeI YGGCCR 1 cut(s) 920
Eam1104I CTCTTC 5 cut(s) 134, 220, 297, 992, 1020
EarI CTCTTC 5 cut(s) 134, 220, 297, 992, 1020
Eco130I CCWWGG 2 cut(s) 220, 463
Eco47I GGWCC 2 cut(s) 460, 928
Eco57I CTGAAG 2 cut(s) 158, 1044
Eco91I GGTNACC 1 cut(s) 206
EcoNI CCTNNNNNAGG 1 cut(s) 447
EcoO109I RGGNCCY 2 cut(s) 460, 928
EcoO65I GGTNACC 1 cut(s) 206
EcoRII CCWGG 2 cut(s) 256, 1230
EcoT14I CCWWGG 2 cut(s) 220, 463
ErhI CCWWGG 2 cut(s) 220, 463
FaeI CATG 9 cut(s) 74, 110, 224, 251, 647, 791, 1045, 1198, 1207
FalI AAGNNNNNCTT 2 cut(s) 396, 428
FaqI GGGAC 4 cut(s) 424, 919, 941, 1115
FatI CATG 9 cut(s) 70, 106, 220, 247, 643, 787, 1041, 1194, 1203
FauI CCCGC 1 cut(s) 489
Fnu4HI GCNGC 4 cut(s) 531, 630, 633, 1253
FokI GGATG 2 cut(s) 581, 1298
Fsp4HI GCNGC 4 cut(s) 531, 630, 633, 1253
FspBI CTAG 2 cut(s) 26, 203
GluI GCNGC 4 cut(s) 531, 630, 633, 1253
HaeIII GGCC 2 cut(s) 453, 922
HgaI GACGC 2 cut(s) 536, 821
Hin1II CATG 9 cut(s) 74, 110, 224, 251, 647, 791, 1045, 1198, 1207
HincII GTYRAC 1 cut(s) 871
HindII GTYRAC 1 cut(s) 871
HinfI GANTC 3 cut(s) 56, 437, 826
HphI GGTGA 2 cut(s) 170, 175
Hpy166II GTNNAC 3 cut(s) 760, 836, 871
Hpy188I TCNGA 5 cut(s) 43, 61, 615, 1264, 1306
Hpy188III TCNNGA 6 cut(s) 26, 53, 471, 644, 950, 1022
Hpy8I GTNNAC 3 cut(s) 760, 836, 871
Hpy99I CGWCG 1 cut(s) 1260
HpyAV CCTTC 8 cut(s) 410, 451, 574, 643, 800, 1141, 1168, 1201
HpyCH4III ACNGT 4 cut(s) 575, 764, 889, 1181
HpyCH4IV ACGT 1 cut(s) 7
HpyCH4V TGCA 7 cut(s) 74, 324, 509, 545, 635, 702, 1070
HpyF10VI GCNNNNNNNGC 2 cut(s) 142, 380
HpyF3I CTNAG 3 cut(s) 562, 852, 1314
HpySE526I ACGT 1 cut(s) 7
Hsp92II CATG 9 cut(s) 74, 110, 224, 251, 647, 791, 1045, 1198, 1207
Kzo9I GATC 5 cut(s) 84, 720, 841, 1259, 1269
LmnI GCTCC 1 cut(s) 491
Lsp1109I GCAGC 4 cut(s) 517, 616, 619, 1264
LweI GCATC 1 cut(s) 886
MaeI CTAG 2 cut(s) 26, 203
MaeII ACGT 1 cut(s) 7
MaeIII GTNAC 6 cut(s) 3, 163, 206, 358, 670, 883
MalI GATC 5 cut(s) 86, 722, 843, 1261, 1271
MboI GATC 5 cut(s) 84, 720, 841, 1259, 1269
MhlI GDGCHC 1 cut(s) 376
MluCI AATT 8 cut(s) 35, 294, 309, 329, 354, 1105, 1135, 1215
MlyI GAGTC 1 cut(s) 446
MmeI TCCRAC 1 cut(s) 381
MseI TTAA 4 cut(s) 270, 312, 332, 687
MspA1I CMGCKG 1 cut(s) 629
MspR9I CCNGG 2 cut(s) 258, 1232
Mva1269I GAATGC 1 cut(s) 1017
MvaI CCWGG 2 cut(s) 258, 1232
MwoI GCNNNNNNNGC 2 cut(s) 142, 380
NcoI CCATGG 1 cut(s) 220
NdeII GATC 5 cut(s) 84, 720, 841, 1259, 1269
NlaIII CATG 9 cut(s) 74, 110, 224, 251, 647, 791, 1045, 1198, 1207
NlaIV GGNNCC 3 cut(s) 612, 858, 929
NmeAIII GCCGAG 1 cut(s) 948
NmuCI GTSAC 3 cut(s) 3, 163, 358
PagI TCATGA 1 cut(s) 643
PctI GAATGC 1 cut(s) 1017
PfeI GAWTC 2 cut(s) 56, 826
PkrI GCNGC 4 cut(s) 532, 631, 634, 1254
Ple19I CGATCG 1 cut(s) 723
PleI GAGTC 1 cut(s) 445
PpsI GAGTC 1 cut(s) 445
Ppu21I YACGTR 1 cut(s) 8
PpuMI RGGWCCY 2 cut(s) 460, 928
PshBI ATTAAT 1 cut(s) 332
Psp5II RGGWCCY 2 cut(s) 460, 928
Psp6I CCWGG 2 cut(s) 256, 1230
PspEI GGTNACC 1 cut(s) 206
PspGI CCWGG 2 cut(s) 256, 1230
PspN4I GGNNCC 3 cut(s) 612, 858, 929
PspPI GGNCC 2 cut(s) 460, 928
PspPPI RGGWCCY 2 cut(s) 460, 928
PstI CTGCAG 1 cut(s) 637
PstNI CAGNNNCTG 1 cut(s) 632
PvuI CGATCG 1 cut(s) 723
PvuII CAGCTG 1 cut(s) 629
RsaI GTAC 2 cut(s) 606, 1202
RsaNI GTAC 2 cut(s) 605, 1201
SaqAI TTAA 4 cut(s) 270, 312, 332, 687
SatI GCNGC 4 cut(s) 531, 630, 633, 1253
Sau3AI GATC 5 cut(s) 84, 720, 841, 1259, 1269
Sau96I GGNCC 2 cut(s) 460, 928
SchI GAGTC 1 cut(s) 446
ScrFI CCNGG 2 cut(s) 258, 1232
SduI GDGCHC 1 cut(s) 376
SfaNI GCATC 1 cut(s) 886
SfcI CTRYAG 1 cut(s) 633
SinI GGWCC 2 cut(s) 460, 928
SmlI CTYRAG 1 cut(s) 846
SmoI CTYRAG 1 cut(s) 846
Sse9I AATT 8 cut(s) 35, 294, 309, 329, 354, 1105, 1135, 1215
SsiI CCGC 3 cut(s) 496, 553, 1198
SspI AATATT 2 cut(s) 730, 1036
SspMI CTAG 2 cut(s) 26, 203
StyD4I CCNGG 2 cut(s) 256, 1230
StyI CCWWGG 2 cut(s) 220, 463
TaaI ACNGT 4 cut(s) 575, 764, 889, 1181
TaiI ACGT 1 cut(s) 10
TaqI TCGA 5 cut(s) 66, 696, 719, 723, 824
TasI AATT 8 cut(s) 35, 294, 309, 329, 354, 1105, 1135, 1215
TatI WGTACW 1 cut(s) 604
TfiI GAWTC 2 cut(s) 56, 826
Tru1I TTAA 4 cut(s) 270, 312, 332, 687
Tru9I TTAA 4 cut(s) 270, 312, 332, 687
TscAI CASTG 3 cut(s) 157, 184, 580
TseFI GTSAC 3 cut(s) 3, 163, 358
TseI GCWGC 4 cut(s) 530, 629, 632, 1252
Tsp45I GTSAC 3 cut(s) 3, 163, 358
TspDTI ATGAA 8 cut(s) 632, 776, 974, 1058, 1180, 1220, 1329, 1340
TspRI CASTG 3 cut(s) 157, 184, 580
VpaK11BI GGWCC 2 cut(s) 460, 928
VspI ATTAAT 1 cut(s) 332
XagI CCTNNNNNAGG 1 cut(s) 447
XbaI TCTAGA 1 cut(s) 25
XcmI CCANNNNNNNNNTGG 3 cut(s) 461, 754, 785
XspI CTAG 2 cut(s) 26, 203
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.