Rh4CG304800

source UniProtKB

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4C
OG0000695
Physical Location & Seq
Forward (+)
56565228 .. 56612396
47169 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4CG304800.1

Sequence Viewer

Length: 1341 bp
ATGAAGCTGCATGAAGACGACCTTGTACGCGATCTCGGAGTCACAAATCTGAGGCACCGGCGCCGGCTTTTGACTTGGGTGCCCAGTCGAAACTCGAGCTCTCCTCCAAAAACCTCCAGATCTGGATCTTTTGTTGACTCTTTGTCGGAGGCGAAGACCATTATCAGGGATTACATCTCCAAGCATCCAGAGGAAGTAGCTGAAACATCTGATATAAAAATACCTGCATGGACAACTGGTGTTGCGGAATGGGGAAAGAAAGATGAAAGCGAGGTAGTTGAGATTCCAAGAGGCCAGCCTACCCCATGCCTTCCTCTCCCAAAGGACCTTAAACATGTAAGTGCAGCTACAGCTTCCACGCTACTGGATATGATTAGTGGTTTGAATGTCCTTCTATTGCATAAGTGCATGGTAGGAAAGGATGAGATCAAAAGGCTCAGGAAGGAGAATGAAGAGCTCCGTGCAAGTGTAAAGATGCTTTCGATCATGGCTCCTTCAAAATCTAGGGCTGCCACTAGAGCAAGGCAGAATATTATCAATGCATTGAAAGTGACAAGAGCGAGATCTGCACCAGAACTAGCATATTCTGGTCAGTCAAATGCATCAGAAAATAAGCCATCACCAAAGAGAGCAACGACATCGTTAAGGGTCAGCAAGTCATTGACAAAGCATAAGAAGAAAAAGTCATCTAAGGCATCCAGCCCAGAAAAAAGCGGCTTAAAGTTGCTGAAGCGTGGTTGTGTTACAATGCACAGGATTGTGAGAAGGAAAATTCTTGGGTCTGAAATCAATCCTGATTCGGAAATCGACCGTGCAACAATGTGGATAAAGGCACGGCAGGATAAACACGGCAACTTCAAAGATGTTGAGGTAGAGAAGTGTGCCGCAGACATAGAAGTGGAAGAAGATCAGGTTGTACAGGTTCCGGTGGAAGAACCAGCTGCTGTAGTAGTTCATGAGAAGGCTATTGAGTTGGTTGAAGTTACACAGCAGAAACAACCAAATAAGTCCAGTAAGGGTCTGAAGAAGAGGAAAATGCAATTAGACAGTCCTGAAGAGGTGAATATTGACATGAAGAAGCTACCAAAAGATTACCCTGCACCTTTGAAGTGCTTGTGGATTTGGACAAGGGACAATTTGGCAAATGGGAGGACAATCTCCTTCAATTTGGAACAGAAAGTGTTTGGCATCGACAGGAAGCATTATCTGTTTAGGTCAGACATTCATGCATTGTGTACAATGACTGAACTATCAGGTGGAGTAATCTCAATGTATATATGTATAGGACTAGCCACTGCTCATTCATTAATTGAACAACCTCAAAAGGCATTTTTAGCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Peptidase_C48 Transposase_24

Protein Analysis

446

Amino Acids

49.98

Weight (kDa)

9.62

Isoelectric Point (pI)

58.33

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 232
AccB1I GGYRCC 3 cut(s) 54, 60, 79
AccII CGCG 1 cut(s) 30
AciI CCGC 3 cut(s) 245, 714, 885
AclWI GGATC 1 cut(s) 133
AcsI RAATTY 1 cut(s) 771
AcuI CTGAAG 3 cut(s) 749, 1043, 1074
AcyI GRCGYC 1 cut(s) 61
AfaI GTAC 3 cut(s) 27, 918, 1237
AfiI CCNNNNNNNGG 1 cut(s) 165
AflIII ACRYGT 1 cut(s) 334
AgsI TTSAA 8 cut(s) 385, 498, 547, 859, 980, 1108, 1165, 1313
AhdI GACNNNNNGTC 1 cut(s) 142
AluBI AGCT 9 cut(s) 7, 99, 200, 347, 353, 457, 941, 1081, 1337
AluI AGCT 9 cut(s) 7, 99, 200, 347, 353, 457, 941, 1081, 1337
Alw21I GWGCWC 2 cut(s) 101, 459
AlwI GGATC 1 cut(s) 133
AlwNI CAGNNNCTG 1 cut(s) 944
Ama87I CYCGRG 1 cut(s) 94
AoxI GGCC 1 cut(s) 292
ApeKI GCWGC 4 cut(s) 7, 344, 509, 941
ApoI RAATTY 1 cut(s) 771
AseI ATTAAT 1 cut(s) 1307
AspLEI GCGC 1 cut(s) 63
AspS9I GGNCC 1 cut(s) 325
AsuHPI GGTGA 2 cut(s) 612, 1072
AvaI CYCGRG 1 cut(s) 94
AvaII GGWCC 1 cut(s) 325
BaeGI GKGCMC 1 cut(s) 84
BanI GGYRCC 3 cut(s) 54, 60, 79
BanII GRGCYC 2 cut(s) 101, 459
BbsI GAAGAC 2 cut(s) 21, 161
Bbv12I GWGCWC 2 cut(s) 101, 459
BbvI GCAGC 3 cut(s) 356, 496, 928
BccI CCATC 1 cut(s) 625
BceAI ACGGC 2 cut(s) 851, 865
BcgI CGANNNNNNTGC 2 cut(s) 621, 655
BfaI CTAG 4 cut(s) 504, 516, 578, 1289
BfmI CTRYAG 2 cut(s) 348, 945
BfoI RGCGCY 1 cut(s) 64
BfuAI ACCTGC 1 cut(s) 232
BglII AGATCT 2 cut(s) 119, 563
BisI GCNGC 6 cut(s) 8, 345, 510, 715, 885, 942
BlsI GCNGC 6 cut(s) 9, 346, 511, 716, 886, 943
Bme18I GGWCC 1 cut(s) 325
BmeRI GACNNNNNGTC 1 cut(s) 142
BmeT110I CYCGRG 1 cut(s) 94
BmgT120I GGNCC 1 cut(s) 325
BmiI GGNNCC 5 cut(s) 56, 62, 81, 492, 924
BmrI ACTGGG 1 cut(s) 78
BmsI GCATC 5 cut(s) 193, 465, 611, 704, 1197
BmuI ACTGGG 1 cut(s) 78
BpiI GAAGAC 2 cut(s) 21, 161
BplI GAGNNNNNCTC 2 cut(s) 88, 120
BpmI CTGGAG 1 cut(s) 100
Bpu10I CCTNAGC 1 cut(s) 437
BsaBI GATNNNNATC 1 cut(s) 124
BsaHI GRCGYC 1 cut(s) 61
BsaWI WCCGGW 1 cut(s) 925
Bsc4I CCNNNNNNNGG 1 cut(s) 165
Bse118I RCCGGY 2 cut(s) 57, 63
Bse1I ACTGG 4 cut(s) 84, 241, 369, 1011
Bse8I GATNNNNATC 1 cut(s) 124
BseGI GGATG 3 cut(s) 184, 427, 695
BseJI GATNNNNATC 1 cut(s) 124
BseLI CCNNNNNNNGG 1 cut(s) 165
BseMII CTCAG 2 cut(s) 41, 451
BseNI ACTGG 4 cut(s) 84, 241, 369, 1011
BseRI GAGGAG 1 cut(s) 93
BseSI GKGCMC 1 cut(s) 84
BseXI GCAGC 3 cut(s) 356, 496, 928
BsgI GTGCAG 3 cut(s) 363, 552, 1083
Bsh1236I CGCG 1 cut(s) 30
Bsh1285I CGRYCG 1 cut(s) 811
BshFI GGCC 1 cut(s) 294
BshNI GGYRCC 3 cut(s) 54, 60, 79
BsiEI CGRYCG 1 cut(s) 811
BsiHKAI GWGCWC 2 cut(s) 101, 459
BsiHKCI CYCGRG 1 cut(s) 94
BsiSI CCGG 3 cut(s) 58, 64, 926
BslFI GGGAC 1 cut(s) 1145
BslI CCNNNNNNNGG 1 cut(s) 165
BsmFI GGGAC 1 cut(s) 1145
BsnI GGCC 1 cut(s) 294
BsoBI CYCGRG 1 cut(s) 94
Bsp1286I GDGCHC 3 cut(s) 84, 101, 459
Bsp1407I TGTACA 2 cut(s) 916, 1235
Bsp143I GATC 7 cut(s) 31, 119, 125, 426, 483, 563, 907
BspACI CCGC 3 cut(s) 245, 714, 885
BspANI GGCC 1 cut(s) 294
BspCNI CTCAG 2 cut(s) 42, 450
BspFNI CGCG 1 cut(s) 30
BspHI TCATGA 1 cut(s) 955
BspLI GGNNCC 5 cut(s) 56, 62, 81, 492, 924
BspMI ACCTGC 1 cut(s) 232
BspPI GGATC 1 cut(s) 133
BspQI GCTCTTC 1 cut(s) 447
BspT107I GGYRCC 3 cut(s) 54, 60, 79
BsrFI RCCGGY 2 cut(s) 57, 63
BsrGI TGTACA 2 cut(s) 916, 1235
BsrI ACTGG 4 cut(s) 84, 241, 369, 1011
BssAI RCCGGY 2 cut(s) 57, 63
BssMI GATC 7 cut(s) 31, 119, 125, 426, 483, 563, 907
BssNI GRCGYC 1 cut(s) 61
Bst4CI ACNGT 2 cut(s) 812, 1049
Bst6I CTCTTC 3 cut(s) 447, 1022, 1050
BstACI GRCGYC 1 cut(s) 61
BstAUI TGTACA 2 cut(s) 916, 1235
BstC8I GCNNGC 2 cut(s) 65, 296
BstDEI CTNAG 3 cut(s) 50, 437, 690
BstF5I GGATG 3 cut(s) 184, 427, 695
BstFNI CGCG 1 cut(s) 30
BstH2I RGCGCY 1 cut(s) 64
BstHHI GCGC 1 cut(s) 63
BstKTI GATC 7 cut(s) 34, 122, 128, 429, 486, 566, 910
BstMBI GATC 7 cut(s) 31, 119, 125, 426, 483, 563, 907
BstMCI CGRYCG 1 cut(s) 811
BstMWI GCNNNNNNNGC 4 cut(s) 350, 518, 566, 1334
BstNSI RCATGY 1 cut(s) 338
BstSFI CTRYAG 2 cut(s) 348, 945
BstSLI GKGCMC 1 cut(s) 84
BstUI CGCG 1 cut(s) 30
BstV1I GCAGC 3 cut(s) 356, 496, 928
BstV2I GAAGAC 2 cut(s) 21, 161
BstX2I RGATCY 3 cut(s) 119, 125, 563
BstXI CCANNNNNNTGG 1 cut(s) 364
BstYI RGATCY 3 cut(s) 119, 125, 563
BsuRI GGCC 1 cut(s) 294
BtsCI GGATG 3 cut(s) 184, 427, 695
BtsI GCAGTG 1 cut(s) 1293
BtsIMutI CAGTG 1 cut(s) 1293
BveI ACCTGC 1 cut(s) 232
Cac8I GCNNGC 2 cut(s) 65, 296
CaiI CAGNNNCTG 1 cut(s) 944
CciI TCATGA 1 cut(s) 955
CfoI GCGC 1 cut(s) 63
Cfr10I RCCGGY 2 cut(s) 57, 63
Cfr13I GGNCC 1 cut(s) 325
Csp6I GTAC 3 cut(s) 26, 917, 1236
CviAII CATG 9 cut(s) 11, 228, 306, 335, 409, 487, 956, 1072, 1226
CviQI GTAC 3 cut(s) 26, 917, 1236
DdeI CTNAG 3 cut(s) 50, 437, 690
DinI GGCGCC 1 cut(s) 62
DpnI GATC 7 cut(s) 33, 121, 127, 428, 485, 565, 909
DpnII GATC 7 cut(s) 31, 119, 125, 426, 483, 563, 907
DriI GACNNNNNGTC 1 cut(s) 142
Eam1104I CTCTTC 3 cut(s) 447, 1022, 1050
Eam1105I GACNNNNNGTC 1 cut(s) 142
EarI CTCTTC 3 cut(s) 447, 1022, 1050
Ecl136II GAGCTC 2 cut(s) 99, 457
Eco24I GRGCYC 2 cut(s) 101, 459
Eco47I GGWCC 1 cut(s) 325
Eco53kI GAGCTC 2 cut(s) 99, 457
Eco57I CTGAAG 3 cut(s) 749, 1043, 1074
Eco88I CYCGRG 1 cut(s) 94
EcoICRI GAGCTC 2 cut(s) 99, 457
EcoO109I RGGNCCY 1 cut(s) 325
EcoT22I ATGCAT 3 cut(s) 544, 604, 1231
EcoT38I GRGCYC 2 cut(s) 101, 459
EgeI GGCGCC 1 cut(s) 62
EheI GGCGCC 1 cut(s) 62
FaeI CATG 9 cut(s) 14, 231, 309, 338, 412, 490, 959, 1075, 1229
FalI AAGNNNNNCTT 1 cut(s) 38
FaqI GGGAC 1 cut(s) 1145
FatI CATG 9 cut(s) 10, 227, 305, 334, 408, 486, 955, 1071, 1225
Fnu4HI GCNGC 6 cut(s) 8, 345, 510, 715, 885, 942
FokI GGATG 3 cut(s) 171, 434, 682
FriOI GRGCYC 2 cut(s) 101, 459
Fsp4HI GCNGC 6 cut(s) 8, 345, 510, 715, 885, 942
FspBI CTAG 4 cut(s) 504, 516, 578, 1289
GlaI GCGC 1 cut(s) 62
GluI GCNGC 6 cut(s) 8, 345, 510, 715, 885, 942
GsuI CTGGAG 1 cut(s) 100
HaeII RGCGCY 1 cut(s) 64
HaeIII GGCC 1 cut(s) 294
HapII CCGG 3 cut(s) 58, 64, 926
HhaI GCGC 1 cut(s) 63
Hin1I GRCGYC 1 cut(s) 61
Hin1II CATG 9 cut(s) 14, 231, 309, 338, 412, 490, 959, 1075, 1229
Hin6I GCGC 1 cut(s) 61
HinP1I GCGC 1 cut(s) 61
HincII GTYRAC 1 cut(s) 136
HindII GTYRAC 1 cut(s) 136
HinfI GANTC 4 cut(s) 39, 137, 283, 797
HpaII CCGG 3 cut(s) 58, 64, 926
HphI GGTGA 2 cut(s) 612, 1072
Hpy166II GTNNAC 2 cut(s) 136, 1236
Hpy188I TCNGA 9 cut(s) 38, 51, 148, 211, 607, 784, 802, 1023, 1219
Hpy188III TCNNGA 7 cut(s) 117, 123, 188, 439, 794, 956, 1052
Hpy8I GTNNAC 2 cut(s) 136, 1236
HpyAV CCTTC 7 cut(s) 320, 401, 436, 504, 759, 955, 1171
HpyCH4III ACNGT 2 cut(s) 812, 1049
HpyF10VI GCNNNNNNNGC 4 cut(s) 350, 518, 566, 1334
HpyF3I CTNAG 3 cut(s) 50, 437, 690
Hsp92I GRCGYC 1 cut(s) 61
Hsp92II CATG 9 cut(s) 14, 231, 309, 338, 412, 490, 959, 1075, 1229
HspAI GCGC 1 cut(s) 61
KasI GGCGCC 1 cut(s) 60
KroI GCCGGC 1 cut(s) 63
KroNI GCCGGC 1 cut(s) 65
Kzo9I GATC 7 cut(s) 31, 119, 125, 426, 483, 563, 907
LguI GCTCTTC 1 cut(s) 447
LmnI GCTCC 2 cut(s) 462, 496
Lsp1109I GCAGC 3 cut(s) 356, 496, 928
LweI GCATC 5 cut(s) 193, 465, 611, 704, 1197
MaeI CTAG 4 cut(s) 504, 516, 578, 1289
MaeIII GTNAC 4 cut(s) 40, 550, 742, 982
MalI GATC 7 cut(s) 33, 121, 127, 428, 485, 565, 909
MboI GATC 7 cut(s) 31, 119, 125, 426, 483, 563, 907
MflI RGATCY 3 cut(s) 119, 125, 563
MhlI GDGCHC 3 cut(s) 84, 101, 459
MluCI AATT 5 cut(s) 771, 1040, 1135, 1165, 1308
Mly113I GGCGCC 1 cut(s) 61
MlyI GAGTC 2 cut(s) 48, 131
MmeI TCCRAC 1 cut(s) 126
Mph1103I ATGCAT 3 cut(s) 544, 604, 1231
MroNI GCCGGC 1 cut(s) 63
MseI TTAA 5 cut(s) 330, 644, 719, 1307, 1339
MslI CAYNNNNRTG 2 cut(s) 339, 896
MspA1I CMGCKG 1 cut(s) 941
MspI CCGG 3 cut(s) 58, 64, 926
MvnI CGCG 1 cut(s) 30
MwoI GCNNNNNNNGC 4 cut(s) 350, 518, 566, 1334
NaeI GCCGGC 1 cut(s) 65
NarI GGCGCC 1 cut(s) 61
NdeII GATC 7 cut(s) 31, 119, 125, 426, 483, 563, 907
NgoMIV GCCGGC 1 cut(s) 63
NlaIII CATG 9 cut(s) 14, 231, 309, 338, 412, 490, 959, 1075, 1229
NlaIV GGNNCC 5 cut(s) 56, 62, 81, 492, 924
NmuCI GTSAC 2 cut(s) 40, 550
NsiI ATGCAT 3 cut(s) 544, 604, 1231
NspI RCATGY 1 cut(s) 338
PaeR7I CTCGAG 1 cut(s) 94
PagI TCATGA 1 cut(s) 955
PciI ACATGT 1 cut(s) 334
PciSI GCTCTTC 1 cut(s) 447
PdiI GCCGGC 1 cut(s) 65
PfeI GAWTC 2 cut(s) 283, 797
PkrI GCNGC 6 cut(s) 9, 346, 511, 716, 886, 943
PleI GAGTC 2 cut(s) 47, 131
PluTI GGCGCC 1 cut(s) 64
PpsI GAGTC 2 cut(s) 47, 131
PpuMI RGGWCCY 1 cut(s) 325
PscI ACATGT 1 cut(s) 334
PshBI ATTAAT 1 cut(s) 1307
Psp124BI GAGCTC 2 cut(s) 101, 459
Psp5II RGGWCCY 1 cut(s) 325
PspN4I GGNNCC 5 cut(s) 56, 62, 81, 492, 924
PspPI GGNCC 1 cut(s) 325
PspPPI RGGWCCY 1 cut(s) 325
PspXI VCTCGAGB 1 cut(s) 94
PstNI CAGNNNCTG 1 cut(s) 944
PsuI RGATCY 3 cut(s) 119, 125, 563
PvuII CAGCTG 1 cut(s) 941
RsaI GTAC 3 cut(s) 27, 918, 1237
RsaNI GTAC 3 cut(s) 26, 917, 1236
RseI CAYNNNNRTG 2 cut(s) 339, 896
SacI GAGCTC 2 cut(s) 101, 459
SapI GCTCTTC 1 cut(s) 447
SaqAI TTAA 5 cut(s) 330, 644, 719, 1307, 1339
SatI GCNGC 6 cut(s) 8, 345, 510, 715, 885, 942
Sau3AI GATC 7 cut(s) 31, 119, 125, 426, 483, 563, 907
Sau96I GGNCC 1 cut(s) 325
SchI GAGTC 2 cut(s) 48, 131
SduI GDGCHC 3 cut(s) 84, 101, 459
SfaNI GCATC 5 cut(s) 193, 465, 611, 704, 1197
SfcI CTRYAG 2 cut(s) 348, 945
SfoI GGCGCC 1 cut(s) 62
Sfr274I CTCGAG 1 cut(s) 94
SgrAI CRCCGGYG 1 cut(s) 57
SinI GGWCC 1 cut(s) 325
SlaI CTCGAG 1 cut(s) 94
SmiMI CAYNNNNRTG 2 cut(s) 339, 896
SmlI CTYRAG 1 cut(s) 94
SmoI CTYRAG 1 cut(s) 94
Sse9I AATT 5 cut(s) 771, 1040, 1135, 1165, 1308
SsiI CCGC 3 cut(s) 245, 714, 885
SspDI GGCGCC 1 cut(s) 60
SspI AATATT 2 cut(s) 532, 1066
SspMI CTAG 4 cut(s) 504, 516, 578, 1289
SstI GAGCTC 2 cut(s) 101, 459
TaaI ACNGT 2 cut(s) 812, 1049
TaqI TCGA 5 cut(s) 88, 95, 482, 807, 1191
TasI AATT 5 cut(s) 771, 1040, 1135, 1165, 1308
TatI WGTACW 2 cut(s) 916, 1235
TauI GCSGC 2 cut(s) 717, 887
TfiI GAWTC 2 cut(s) 283, 797
Tru1I TTAA 5 cut(s) 330, 644, 719, 1307, 1339
Tru9I TTAA 5 cut(s) 330, 644, 719, 1307, 1339
TscAI CASTG 1 cut(s) 1300
TseFI GTSAC 2 cut(s) 40, 550
TseI GCWGC 4 cut(s) 7, 344, 509, 941
Tsp45I GTSAC 2 cut(s) 40, 550
TspDTI ATGAA 8 cut(s) 17, 27, 279, 465, 944, 1088, 1214, 1293
TspGWI ACGGA 1 cut(s) 449
TspRI CASTG 1 cut(s) 1300
VpaK11BI GGWCC 1 cut(s) 325
VspI ATTAAT 1 cut(s) 1307
XapI RAATTY 1 cut(s) 771
XceI RCATGY 1 cut(s) 338
XhoI CTCGAG 1 cut(s) 94
XspI CTAG 4 cut(s) 504, 516, 578, 1289
Zsp2I ATGCAT 3 cut(s) 544, 604, 1231
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.