RLG00000013435

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr3
OG0000695
Physical Location & Seq
Forward (+)
31494395 .. 31497005
2611 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000013435

Sequence Viewer

Length: 804 bp
ATGGCTCCTTCAAAGACATTGGCTGCCTCTAGAGCAAAGAAAAATATTCTTAAGGCACTGAGAGCTACAAGAGCGAGGTCTGCACCAGTAGCAGCAAAATCTGGTCCATCGAATGCTTCTGAAAATAATACATCCCTAAAGAAAGCAATGATGTCGCTGAAGGTGAACAAGACATTGACCAAGAAGAAGGGCAAATCATCGAAGGCCACCAGCCCCTCACCAACCGGCTTAAAGTTGCTGAAATATGGGAGAACCCTTCCCATTGAGTCGCCTCTAGCGGGCCATGTTACTAAGGACCTTGGTTCTGGACAAGGTAGCAGCTCCAATTTGCGGGAGAAATGTGCTCTTCAAAGGGCTGAAGATCTTGTTGTCAATGCAAAGAAGCATTTGGAGTTAGAGGATGACGTTCAAGAGAAAATACAAGAAGAAAATGTTGTAAAGCTATTGGTGGAAGAACTAGTGCCTATAGAGGTTGAAGAAAAGGCTATTCAGTTGGTGGAAGTCACACAACAGAAACAACGGCCTAAGTCTGTTAAGGGTCTGAAGAAGAGGAAAAGGCCAGTAGACAAGGATGAAGAGGAGGATCTCGACTTGAAGAAACTGCCAAAAGATTACCCTTCACCGTTGAAATGCTTGTGGTTATGGGCTAGAGACAATTTGGCAAATGGGAGGACAATGTCCTTTCAGATGGAAGATAAAGTTTTTGGCATCAACAGGAAGCAATATTTGTTTAAGGGAGACATTTATGCATTGTGTACCATGTCTGAACTGTTTAGTGGAGTGATCTCCATGTATATTCTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

268

Amino Acids

29.72

Weight (kDa)

9.72

Isoelectric Point (pI)

58.32

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 564
AciI CCGC 2 cut(s) 278, 331
AclWI GGATC 1 cut(s) 591
AcuI CTGAAG 3 cut(s) 179, 378, 563
AfaI GTAC 1 cut(s) 757
AfiI CCNNNNNNNGG 2 cut(s) 278, 330
AflII CTTAAG 1 cut(s) 50
AgsI TTSAA 6 cut(s) 12, 350, 410, 476, 595, 628
AhlI ACTAGT 1 cut(s) 457
AluBI AGCT 3 cut(s) 65, 321, 442
AluI AGCT 3 cut(s) 65, 321, 442
Alw21I GWGCWC 1 cut(s) 346
Alw26I GTCTC 2 cut(s) 645, 732
AlwI GGATC 1 cut(s) 591
AoxI GGCC 4 cut(s) 204, 280, 521, 557
ApeKI GCWGC 3 cut(s) 23, 92, 318
AspS9I GGNCC 3 cut(s) 104, 280, 295
AsuHPI GGTGA 3 cut(s) 175, 210, 612
AvaII GGWCC 2 cut(s) 104, 295
Bbv12I GWGCWC 1 cut(s) 346
BbvI GCAGC 3 cut(s) 10, 104, 330
BccI CCATC 2 cut(s) 115, 682
BceAI ACGGC 1 cut(s) 536
BcgI CGANNNNNNTGC 2 cut(s) 135, 169
BcoDI GTCTC 2 cut(s) 645, 732
BcuI ACTAGT 1 cut(s) 457
BfaI CTAG 4 cut(s) 30, 275, 458, 648
BfmI CTRYAG 1 cut(s) 465
BfrI CTTAAG 1 cut(s) 50
BglII AGATCT 1 cut(s) 361
BisI GCNGC 3 cut(s) 24, 93, 319
BlsI GCNGC 3 cut(s) 25, 94, 320
Bme18I GGWCC 2 cut(s) 104, 295
BmgT120I GGNCC 3 cut(s) 104, 280, 295
BmiI GGNNCC 1 cut(s) 6
BmsI GCATC 1 cut(s) 717
BsaJI CCNNGG 1 cut(s) 298
Bsc4I CCNNNNNNNGG 2 cut(s) 278, 330
Bse118I RCCGGY 1 cut(s) 224
Bse1I ACTGG 2 cut(s) 86, 560
Bse3DI GCAATG 1 cut(s) 153
BseDI CCNNGG 1 cut(s) 298
BseGI GGATG 3 cut(s) 131, 406, 577
BseLI CCNNNNNNNGG 2 cut(s) 278, 330
BseMI GCAATG 1 cut(s) 153
BseMII CTCAG 1 cut(s) 50
BseNI ACTGG 2 cut(s) 86, 560
BseRI GAGGAG 1 cut(s) 593
BseXI GCAGC 3 cut(s) 10, 104, 330
BsgI GTGCAG 1 cut(s) 66
BshFI GGCC 4 cut(s) 206, 282, 523, 559
BsiHKAI GWGCWC 1 cut(s) 346
BsiSI CCGG 1 cut(s) 225
BslI CCNNNNNNNGG 2 cut(s) 278, 330
BsmAI GTCTC 2 cut(s) 645, 732
BsmI GAATGC 1 cut(s) 118
BsnI GGCC 4 cut(s) 206, 282, 523, 559
Bsp1286I GDGCHC 1 cut(s) 346
Bsp143I GATC 3 cut(s) 361, 583, 783
BspACI CCGC 2 cut(s) 278, 331
BspANI GGCC 4 cut(s) 206, 282, 523, 559
BspCNI CTCAG 1 cut(s) 51
BspLI GGNNCC 1 cut(s) 6
BspPI GGATC 1 cut(s) 591
BspQI GCTCTTC 1 cut(s) 351
BspTI CTTAAG 1 cut(s) 50
BsrDI GCAATG 1 cut(s) 153
BsrFI RCCGGY 1 cut(s) 224
BsrI ACTGG 2 cut(s) 86, 560
BssAI RCCGGY 1 cut(s) 224
BssECI CCNNGG 1 cut(s) 298
BssMI GATC 3 cut(s) 361, 583, 783
BssT1I CCWWGG 1 cut(s) 298
Bst4CI ACNGT 2 cut(s) 624, 771
Bst6I CTCTTC 3 cut(s) 351, 542, 570
BstAFI CTTAAG 1 cut(s) 50
BstC8I GCNNGC 1 cut(s) 280
BstDEI CTNAG 3 cut(s) 59, 291, 525
BstF5I GGATG 3 cut(s) 131, 406, 577
BstKTI GATC 3 cut(s) 364, 586, 786
BstMAI GTCTC 2 cut(s) 645, 732
BstMBI GATC 3 cut(s) 361, 583, 783
BstMWI GCNNNNNNNGC 5 cut(s) 32, 62, 71, 80, 89
BstSFI CTRYAG 1 cut(s) 465
BstV1I GCAGC 3 cut(s) 10, 104, 330
BstX2I RGATCY 2 cut(s) 361, 583
BstYI RGATCY 2 cut(s) 361, 583
BsuRI GGCC 4 cut(s) 206, 282, 523, 559
BtsCI GGATG 3 cut(s) 131, 406, 577
BtsIMutI CAGTG 1 cut(s) 56
Cac8I GCNNGC 1 cut(s) 280
Cfr10I RCCGGY 1 cut(s) 224
Cfr13I GGNCC 3 cut(s) 104, 280, 295
Csp6I GTAC 1 cut(s) 756
CviAII CATG 3 cut(s) 284, 760, 790
CviQI GTAC 1 cut(s) 756
DdeI CTNAG 3 cut(s) 59, 291, 525
DpnI GATC 3 cut(s) 363, 585, 785
DpnII GATC 3 cut(s) 361, 583, 783
Eam1104I CTCTTC 3 cut(s) 351, 542, 570
EarI CTCTTC 3 cut(s) 351, 542, 570
Eco130I CCWWGG 1 cut(s) 298
Eco47I GGWCC 2 cut(s) 104, 295
Eco57I CTGAAG 3 cut(s) 179, 378, 563
EcoO109I RGGNCCY 1 cut(s) 295
EcoT14I CCWWGG 1 cut(s) 298
EcoT22I ATGCAT 1 cut(s) 751
ErhI CCWWGG 1 cut(s) 298
FaeI CATG 3 cut(s) 287, 763, 793
FaiI YATR 8 cut(s) 246, 285, 467, 643, 747, 761, 791, 795
FatI CATG 3 cut(s) 283, 759, 789
FauI CCCGC 2 cut(s) 271, 324
FblI GTMKAC 1 cut(s) 564
Fnu4HI GCNGC 3 cut(s) 24, 93, 319
FokI GGATG 3 cut(s) 118, 413, 584
Fsp4HI GCNGC 3 cut(s) 24, 93, 319
FspBI CTAG 4 cut(s) 30, 275, 458, 648
GluI GCNGC 3 cut(s) 24, 93, 319
HaeIII GGCC 4 cut(s) 206, 282, 523, 559
HapII CCGG 1 cut(s) 225
Hin1II CATG 3 cut(s) 287, 763, 793
HinfI GANTC 1 cut(s) 266
HpaII CCGG 1 cut(s) 225
HphI GGTGA 3 cut(s) 175, 210, 612
Hpy166II GTNNAC 3 cut(s) 166, 565, 756
Hpy188I TCNGA 4 cut(s) 121, 543, 687, 766
Hpy188III TCNNGA 4 cut(s) 30, 306, 410, 587
Hpy8I GTNNAC 3 cut(s) 166, 565, 756
HpyAV CCTTC 6 cut(s) 18, 154, 181, 196, 266, 627
HpyCH4III ACNGT 2 cut(s) 624, 771
HpyCH4IV ACGT 1 cut(s) 405
HpyCH4V TGCA 3 cut(s) 83, 377, 749
HpyF10VI GCNNNNNNNGC 5 cut(s) 32, 62, 71, 80, 89
HpyF3I CTNAG 3 cut(s) 59, 291, 525
HpySE526I ACGT 1 cut(s) 405
Hsp92II CATG 3 cut(s) 287, 763, 793
Kzo9I GATC 3 cut(s) 361, 583, 783
LguI GCTCTTC 1 cut(s) 351
LmnI GCTCC 2 cut(s) 10, 326
LpnPI CCDG 7 cut(s) 87, 99, 223, 238, 291, 573, 700
Lsp1109I GCAGC 3 cut(s) 10, 104, 330
LweI GCATC 1 cut(s) 717
MaeI CTAG 4 cut(s) 30, 275, 458, 648
MaeII ACGT 1 cut(s) 405
MaeIII GTNAC 2 cut(s) 286, 502
MalI GATC 3 cut(s) 363, 585, 785
MboI GATC 3 cut(s) 361, 583, 783
MflI RGATCY 2 cut(s) 361, 583
MhlI GDGCHC 1 cut(s) 346
MluCI AATT 2 cut(s) 325, 655
MlyI GAGTC 1 cut(s) 275
Mph1103I ATGCAT 1 cut(s) 751
MseI TTAA 4 cut(s) 51, 230, 534, 732
MspCI CTTAAG 1 cut(s) 50
MspI CCGG 1 cut(s) 225
Mva1269I GAATGC 1 cut(s) 118
MwoI GCNNNNNNNGC 5 cut(s) 32, 62, 71, 80, 89
NdeII GATC 3 cut(s) 361, 583, 783
NlaIII CATG 3 cut(s) 287, 763, 793
NlaIV GGNNCC 1 cut(s) 6
NmuCI GTSAC 1 cut(s) 502
NsiI ATGCAT 1 cut(s) 751
PciSI GCTCTTC 1 cut(s) 351
PctI GAATGC 1 cut(s) 118
PflFI GACNNNGTC 1 cut(s) 676
PkrI GCNGC 3 cut(s) 25, 94, 320
PleI GAGTC 1 cut(s) 274
PpsI GAGTC 1 cut(s) 274
PpuMI RGGWCCY 1 cut(s) 295
Psp5II RGGWCCY 1 cut(s) 295
PspN4I GGNNCC 1 cut(s) 6
PspPI GGNCC 3 cut(s) 104, 280, 295
PspPPI RGGWCCY 1 cut(s) 295
PsuI RGATCY 2 cut(s) 361, 583
PsyI GACNNNGTC 1 cut(s) 676
RsaI GTAC 1 cut(s) 757
RsaNI GTAC 1 cut(s) 756
SapI GCTCTTC 1 cut(s) 351
SaqAI TTAA 4 cut(s) 51, 230, 534, 732
SatI GCNGC 3 cut(s) 24, 93, 319
Sau3AI GATC 3 cut(s) 361, 583, 783
Sau96I GGNCC 3 cut(s) 104, 280, 295
SchI GAGTC 1 cut(s) 275
SduI GDGCHC 1 cut(s) 346
SetI ASST 9 cut(s) 67, 80, 165, 300, 316, 323, 408, 444, 474
SfaNI GCATC 1 cut(s) 717
SfcI CTRYAG 1 cut(s) 465
SinI GGWCC 2 cut(s) 104, 295
SmlI CTYRAG 1 cut(s) 50
SmoI CTYRAG 1 cut(s) 50
SpeI ACTAGT 1 cut(s) 457
Sse9I AATT 2 cut(s) 325, 655
SsiI CCGC 2 cut(s) 278, 331
SspI AATATT 2 cut(s) 46, 725
SspMI CTAG 4 cut(s) 30, 275, 458, 648
StyI CCWWGG 1 cut(s) 298
TaaI ACNGT 2 cut(s) 624, 771
TaiI ACGT 1 cut(s) 408
TaqI TCGA 3 cut(s) 110, 200, 588
TasI AATT 2 cut(s) 325, 655
Tru1I TTAA 4 cut(s) 51, 230, 534, 732
Tru9I TTAA 4 cut(s) 51, 230, 534, 732
TscAI CASTG 1 cut(s) 63
TseFI GTSAC 1 cut(s) 502
TseI GCWGC 3 cut(s) 23, 92, 318
Tsp45I GTSAC 1 cut(s) 502
TspDTI ATGAA 1 cut(s) 588
TspRI CASTG 1 cut(s) 63
Tth111I GACNNNGTC 1 cut(s) 676
Vha464I CTTAAG 1 cut(s) 50
VpaK11BI GGWCC 2 cut(s) 104, 295
XbaI TCTAGA 1 cut(s) 29
XmiI GTMKAC 1 cut(s) 564
XspI CTAG 4 cut(s) 30, 275, 458, 648
Zsp2I ATGCAT 1 cut(s) 751
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.