Rmu_sc0002521.1_g000050

source UniProtKB

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0002521.1
OG0000695
Physical Location & Seq
Reverse (-)
218297 .. 221465
3169 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0002521.1_g000050.1.cds

Sequence Viewer

Length: 1731 bp
atggaatttgaggtacccccggaggcaaggagaatggtcctatcttcagcttcacagaagtggagagaattcaagagcaagctgactacacagtacatcttcccacataaggatgaacctgaactattagagtatcccccagctgattacaacttcattgagaagtcttattgggacatctttgtagaaatgcacaaggtgcaaaagagaaagaggtctaagaacaaccctcgtcacatgtcgcgtaagggatatgcaaatctggagactgacttggctgaaaccaatcctgattcggaaatcgaccgtgcaacaatgtggatcaaggcacggcaggataaacacggtaacttcaaagatggtgaggtagagaagtgtgctgcagacatagaaaagctgaagatgcaagtgagtgatggtaaggtgacaacttatggcactgatgatgcattgacaaaggctctaggtaaccctgagcaccatggaagagcgcgtggccagaaggcccttgattctagacaaggtagttgctccaatttgcgggagaagaatgcattacaaccgattgaggacgctgctgttatcctagcaaagaagcgattacagttagtggataacattgcggaggaagtggaagaagataaggttgtacatgttccagtggaagaaccagctgctgcaatagctcatgagaaggctgtgaggccggttgaagatacacagcaggaactaaaagagttcgagtacagattggctttagggtcaatcgataatgttgttgccattacaaccattgttgatgtggacactaattgcacaaaccagacacttcatggtgtttctttgggagaagggaatgtgcgagtgtcgattctgtgtccacttaatcctgaagctaaggttccattcccagtcaacgatgagattgttactgtcaatgatgctattgggacttacattgtttggccgagggacctcatacttcaatcccttccccagactacaaagaaacagcaaaataagtccagtaagggcttaaaaaagaggaaaaggcaagtagataatcctgaagaggagtctattgacttgaaaaagctaccaaaagattaccctgcacatttcaagtgcttgtggatttgggcgagagacaatatggcaaatgggaagacaatctccttcaagttggaagagcaagtgtttggcatcgaaaggaagcaatatctgtttaagtcagacattcatgcattgtgtatgatgactgaactatcaggtggtgctgtgtgcttttacagctacttgcatgaagtcttgaagagagcaaagatgacacacatggttagctttgtggacccttcacagattggggcacttggaagtagaaatgcaacggaaatatcgcattttctagcaaataggttcacaactgcagagaagggccagatttttttgttgccgtataatacaggtgagatcaaaatttacaatgctcagagaaataggtctggctggaaatcaataatgtggcaaaacatggggtgtattccggcacaacaaaatgtgaaggattgcggcatattcatcatactgtacatgaagaaaataattgaggataagaacctgagttttgcttcaaagtggcagcgacgatccgaattgatttacacccaaaaggatattgatgctgtgaggttagagtgggctaagtttatgatgaaatatcatatttcatag
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Peptidase_C48 Transposase_24

Protein Analysis

576

Amino Acids

66.03

Weight (kDa)

8.47

Isoelectric Point (pI)

45.12

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 13
AccB1I GGYRCC 1 cut(s) 13
AccII CGCG 2 cut(s) 244, 493
AciI CCGC 3 cut(s) 541, 623, 1569
AclWI GGATC 2 cut(s) 329, 1641
AcoI YGGCCR 2 cut(s) 496, 965
AcsI RAATTY 3 cut(s) 5, 68, 1476
AcuI CTGAAG 4 cut(s) 30, 419, 912, 1089
AdeI CACNNNGTG 1 cut(s) 199
AfaI GTAC 5 cut(s) 15, 95, 651, 746, 1589
AfiI CCNNNNNNNGG 2 cut(s) 109, 540
AflIII ACRYGT 2 cut(s) 237, 652
AgsI TTSAA 9 cut(s) 73, 355, 713, 986, 1090, 1123, 1180, 1312, 1632
AleI CACNNNNGTG 1 cut(s) 58
Alw21I GWGCWC 1 cut(s) 480
Alw26I GTCTC 2 cut(s) 260, 1140
AlwI GGATC 2 cut(s) 329, 1641
AlwNI CAGNNNCTG 1 cut(s) 677
AoxI GGCC 5 cut(s) 496, 504, 704, 965, 1435
ApeKI GCWGC 5 cut(s) 380, 575, 674, 677, 1639
ApoI RAATTY 3 cut(s) 5, 68, 1476
Asp718I GGTACC 1 cut(s) 13
AspLEI GCGC 1 cut(s) 493
AspS9I GGNCC 5 cut(s) 37, 505, 973, 1348, 1435
AsuC2I CCSGG 1 cut(s) 20
AsuHPI GGTGA 3 cut(s) 374, 436, 1478
AvaII GGWCC 3 cut(s) 37, 973, 1348
BaeGI GKGCMC 1 cut(s) 1369
BalI TGGCCA 1 cut(s) 498
BanI GGYRCC 1 cut(s) 13
BbsI GAAGAC 1 cut(s) 1172
Bbv12I GWGCWC 1 cut(s) 480
BbvI GCAGC 5 cut(s) 367, 562, 661, 664, 1651
BccI CCATC 2 cut(s) 353, 410
BceAI ACGGC 2 cut(s) 347, 1438
BciVI GTATCC 1 cut(s) 144
BcnI CCSGG 1 cut(s) 20
BcoDI GTCTC 2 cut(s) 260, 1140
BfaI CTAG 4 cut(s) 464, 516, 587, 1406
BfmI CTRYAG 2 cut(s) 381, 1425
BfuI GTATCC 1 cut(s) 144
BisI GCNGC 6 cut(s) 381, 576, 675, 678, 1570, 1640
BlsI GCNGC 6 cut(s) 382, 577, 676, 679, 1571, 1641
Bme1390I CCNGG 1 cut(s) 20
Bme18I GGWCC 3 cut(s) 37, 973, 1348
BmgT120I GGNCC 5 cut(s) 37, 505, 973, 1348, 1435
BmiI GGNNCC 4 cut(s) 15, 903, 974, 1350
BmrFI CCNGG 1 cut(s) 20
BmrI ACTGGG 1 cut(s) 905
BmsI GCATC 5 cut(s) 393, 436, 931, 1212, 1669
BmuI ACTGGG 1 cut(s) 905
BpiI GAAGAC 1 cut(s) 1172
BpmI CTGGAG 1 cut(s) 284
Bpu10I CCTNAGC 2 cut(s) 474, 897
BpuMI CCSGG 1 cut(s) 20
Bsa29I ATCGAT 1 cut(s) 768
BsaJI CCNNGG 3 cut(s) 18, 481, 968
Bsc4I CCNNNNNNNGG 2 cut(s) 109, 540
Bse118I RCCGGY 1 cut(s) 706
Bse1I ACTGG 3 cut(s) 659, 911, 1026
Bse3DI GCAATG 1 cut(s) 618
BseCI ATCGAT 1 cut(s) 768
BseDI CCNNGG 3 cut(s) 18, 481, 968
BseGI GGATG 1 cut(s) 118
BseLI CCNNNNNNNGG 2 cut(s) 109, 540
BseMI GCAATG 1 cut(s) 618
BseMII CTCAG 3 cut(s) 465, 1502, 1610
BseNI ACTGG 3 cut(s) 659, 911, 1026
BseRI GAGGAG 1 cut(s) 1088
BseSI GKGCMC 1 cut(s) 1369
BseXI GCAGC 5 cut(s) 367, 562, 661, 664, 1651
BseYI CCCAGC 1 cut(s) 139
BsgI GTGCAG 1 cut(s) 1098
Bsh1236I CGCG 2 cut(s) 244, 493
Bsh1285I CGRYCG 1 cut(s) 307
BshFI GGCC 5 cut(s) 498, 506, 706, 967, 1437
BshNI GGYRCC 1 cut(s) 13
BshVI ATCGAT 1 cut(s) 768
BsiEI CGRYCG 1 cut(s) 307
BsiHKAI GWGCWC 1 cut(s) 480
BsiSI CCGG 3 cut(s) 20, 707, 1544
BslFI GGGAC 3 cut(s) 188, 964, 986
BslI CCNNNNNNNGG 2 cut(s) 109, 540
BsmAI GTCTC 2 cut(s) 260, 1140
BsmFI GGGAC 3 cut(s) 188, 964, 986
BsmI GAATGC 1 cut(s) 556
BsnI GGCC 5 cut(s) 498, 506, 706, 967, 1437
Bsp1286I GDGCHC 2 cut(s) 480, 1369
Bsp1407I TGTACA 2 cut(s) 649, 1587
Bsp143I GATC 3 cut(s) 321, 1470, 1646
Bsp19I CCATGG 1 cut(s) 481
BspACI CCGC 3 cut(s) 541, 623, 1569
BspANI GGCC 5 cut(s) 498, 506, 706, 967, 1437
BspCNI CTCAG 3 cut(s) 466, 1501, 1611
BspDI ATCGAT 1 cut(s) 768
BspFNI CGCG 2 cut(s) 244, 493
BspHI TCATGA 1 cut(s) 688
BspLI GGNNCC 4 cut(s) 15, 903, 974, 1350
BspMAI CTGCAG 2 cut(s) 385, 1429
BspPI GGATC 2 cut(s) 329, 1641
BspQI GCTCTTC 2 cut(s) 481, 1182
BspT107I GGYRCC 1 cut(s) 13
BsrDI GCAATG 1 cut(s) 618
BsrFI RCCGGY 1 cut(s) 706
BsrGI TGTACA 2 cut(s) 649, 1587
BsrI ACTGG 3 cut(s) 659, 911, 1026
BssAI RCCGGY 1 cut(s) 706
BssECI CCNNGG 3 cut(s) 18, 481, 968
BssMI GATC 3 cut(s) 321, 1470, 1646
BssT1I CCWWGG 1 cut(s) 481
Bst4CI ACNGT 6 cut(s) 93, 308, 347, 606, 934, 1587
Bst6I CTCTTC 4 cut(s) 481, 1065, 1182, 1307
BstAPI GCANNNNNTGC 1 cut(s) 199
BstAUI TGTACA 2 cut(s) 649, 1587
BstC8I GCNNGC 1 cut(s) 80
BstDEI CTNAG 6 cut(s) 219, 474, 897, 1488, 1619, 1701
BstDSI CCRYGG 1 cut(s) 481
BstEII GGTNACC 1 cut(s) 467
BstF5I GGATG 1 cut(s) 118
BstFNI CGCG 2 cut(s) 244, 493
BstHHI GCGC 1 cut(s) 493
BstKTI GATC 3 cut(s) 324, 1473, 1649
BstMAI GTCTC 2 cut(s) 260, 1140
BstMBI GATC 3 cut(s) 321, 1470, 1646
BstMCI CGRYCG 1 cut(s) 307
BstMWI GCNNNNNNNGC 4 cut(s) 199, 403, 683, 1290
BstNSI RCATGY 2 cut(s) 241, 656
BstPI GGTNACC 1 cut(s) 467
BstSCI CCNGG 1 cut(s) 18
BstSFI CTRYAG 2 cut(s) 381, 1425
BstSLI GKGCMC 1 cut(s) 1369
BstUI CGCG 2 cut(s) 244, 493
BstV1I GCAGC 5 cut(s) 367, 562, 661, 664, 1651
BstV2I GAAGAC 1 cut(s) 1172
Bsu15I ATCGAT 1 cut(s) 768
BsuI GTATCC 1 cut(s) 144
BsuRI GGCC 5 cut(s) 498, 506, 706, 967, 1437
BsuTUI ATCGAT 1 cut(s) 768
BtgI CCRYGG 1 cut(s) 481
BtsCI GGATG 1 cut(s) 118
BtsIMutI CAGTG 2 cut(s) 438, 666
Cac8I GCNNGC 1 cut(s) 80
CaiI CAGNNNCTG 1 cut(s) 677
CciI TCATGA 1 cut(s) 688
CfoI GCGC 1 cut(s) 493
Cfr10I RCCGGY 1 cut(s) 706
Cfr13I GGNCC 5 cut(s) 37, 505, 973, 1348, 1435
ClaI ATCGAT 1 cut(s) 768
CseI GACGC 1 cut(s) 581
Csp6I GTAC 5 cut(s) 14, 94, 650, 745, 1588
CviQI GTAC 5 cut(s) 14, 94, 650, 745, 1588
DdeI CTNAG 6 cut(s) 219, 474, 897, 1488, 1619, 1701
DpnI GATC 3 cut(s) 323, 1472, 1648
DpnII GATC 3 cut(s) 321, 1470, 1646
DraIII CACNNNGTG 1 cut(s) 199
EaeI YGGCCR 2 cut(s) 496, 965
Eam1104I CTCTTC 4 cut(s) 481, 1065, 1182, 1307
EarI CTCTTC 4 cut(s) 481, 1065, 1182, 1307
Eco130I CCWWGG 1 cut(s) 481
Eco47I GGWCC 3 cut(s) 37, 973, 1348
Eco57I CTGAAG 4 cut(s) 30, 419, 912, 1089
Eco91I GGTNACC 1 cut(s) 467
EcoO109I RGGNCCY 2 cut(s) 505, 973
EcoO65I GGTNACC 1 cut(s) 467
EcoRI GAATTC 1 cut(s) 68
EcoT14I CCWWGG 1 cut(s) 481
EcoT22I ATGCAT 3 cut(s) 451, 556, 1246
ErhI CCWWGG 1 cut(s) 481
FaqI GGGAC 3 cut(s) 188, 964, 986
FauI CCCGC 1 cut(s) 534
Fnu4HI GCNGC 6 cut(s) 381, 576, 675, 678, 1570, 1640
FokI GGATG 1 cut(s) 125
Fsp4HI GCNGC 6 cut(s) 381, 576, 675, 678, 1570, 1640
FspBI CTAG 4 cut(s) 464, 516, 587, 1406
GlaI GCGC 1 cut(s) 492
GluI GCNGC 6 cut(s) 381, 576, 675, 678, 1570, 1640
GsaI CCCAGC 1 cut(s) 143
GsuI CTGGAG 1 cut(s) 284
HaeIII GGCC 5 cut(s) 498, 506, 706, 967, 1437
HapII CCGG 3 cut(s) 20, 707, 1544
HgaI GACGC 1 cut(s) 581
HhaI GCGC 1 cut(s) 493
Hin6I GCGC 1 cut(s) 491
HinP1I GCGC 1 cut(s) 491
HincII GTYRAC 1 cut(s) 916
HindII GTYRAC 1 cut(s) 916
HinfI GANTC 4 cut(s) 293, 512, 871, 1076
HpaII CCGG 3 cut(s) 20, 707, 1544
HphI GGTGA 3 cut(s) 374, 436, 1478
Hpy166II GTNNAC 5 cut(s) 805, 881, 916, 1348, 1419
Hpy188I TCNGA 4 cut(s) 298, 1234, 1491, 1651
Hpy188III TCNNGA 8 cut(s) 73, 263, 290, 516, 689, 890, 1067, 1309
Hpy8I GTNNAC 5 cut(s) 805, 881, 916, 1348, 1419
Hpy99I CGWCG 1 cut(s) 1647
HpyAV CCTTC 8 cut(s) 496, 688, 845, 1001, 1186, 1362, 1426, 1555
HpyCH4III ACNGT 6 cut(s) 93, 308, 347, 606, 934, 1587
HpyF10VI GCNNNNNNNGC 4 cut(s) 199, 403, 683, 1290
HpyF3I CTNAG 6 cut(s) 219, 474, 897, 1488, 1619, 1701
HspAI GCGC 1 cut(s) 491
KpnI GGTACC 1 cut(s) 17
Kzo9I GATC 3 cut(s) 321, 1470, 1646
LguI GCTCTTC 2 cut(s) 481, 1182
LmnI GCTCC 1 cut(s) 536
Lsp1109I GCAGC 5 cut(s) 367, 562, 661, 664, 1651
LweI GCATC 5 cut(s) 393, 436, 931, 1212, 1669
MaeI CTAG 4 cut(s) 464, 516, 587, 1406
MaeIII GTNAC 5 cut(s) 233, 347, 424, 467, 928
MalI GATC 3 cut(s) 323, 1472, 1648
MboI GATC 3 cut(s) 321, 1470, 1646
MhlI GDGCHC 2 cut(s) 480, 1369
MlsI TGGCCA 1 cut(s) 498
MluCI AATT 7 cut(s) 5, 68, 535, 811, 1476, 1602, 1652
MluNI TGGCCA 1 cut(s) 498
MlyI GAGTC 1 cut(s) 1085
MmeI TCCRAC 1 cut(s) 1164
Mox20I TGGCCA 1 cut(s) 498
Mph1103I ATGCAT 3 cut(s) 451, 556, 1246
MscI TGGCCA 1 cut(s) 498
MseI TTAA 3 cut(s) 885, 1037, 1227
MslI CAYNNNNRTG 2 cut(s) 58, 111
Msp20I TGGCCA 1 cut(s) 498
MspA1I CMGCKG 2 cut(s) 143, 674
MspI CCGG 3 cut(s) 20, 707, 1544
MspR9I CCNGG 1 cut(s) 20
Mva1269I GAATGC 1 cut(s) 556
MvnI CGCG 2 cut(s) 244, 493
MwoI GCNNNNNNNGC 4 cut(s) 199, 403, 683, 1290
NciI CCSGG 1 cut(s) 20
NcoI CCATGG 1 cut(s) 481
NdeII GATC 3 cut(s) 321, 1470, 1646
NlaIV GGNNCC 4 cut(s) 15, 903, 974, 1350
NmeAIII GCCGAG 1 cut(s) 993
NmuCI GTSAC 2 cut(s) 233, 424
NsiI ATGCAT 3 cut(s) 451, 556, 1246
NspI RCATGY 2 cut(s) 241, 656
OliI CACNNNNGTG 1 cut(s) 58
PagI TCATGA 1 cut(s) 688
PciI ACATGT 2 cut(s) 237, 652
PciSI GCTCTTC 2 cut(s) 481, 1182
PctI GAATGC 1 cut(s) 556
PfeI GAWTC 3 cut(s) 293, 512, 871
PkrI GCNGC 6 cut(s) 382, 577, 676, 679, 1571, 1641
PleI GAGTC 1 cut(s) 1084
PpsI GAGTC 1 cut(s) 1084
PpuMI RGGWCCY 1 cut(s) 973
PscI ACATGT 2 cut(s) 237, 652
Psp5II RGGWCCY 1 cut(s) 973
PspEI GGTNACC 1 cut(s) 467
PspFI CCCAGC 1 cut(s) 139
PspN4I GGNNCC 4 cut(s) 15, 903, 974, 1350
PspPI GGNCC 5 cut(s) 37, 505, 973, 1348, 1435
PspPPI RGGWCCY 1 cut(s) 973
PstI CTGCAG 2 cut(s) 385, 1429
PstNI CAGNNNCTG 1 cut(s) 677
PvuII CAGCTG 2 cut(s) 143, 674
RsaI GTAC 5 cut(s) 15, 95, 651, 746, 1589
RsaNI GTAC 5 cut(s) 14, 94, 650, 745, 1588
RseI CAYNNNNRTG 2 cut(s) 58, 111
SapI GCTCTTC 2 cut(s) 481, 1182
SaqAI TTAA 3 cut(s) 885, 1037, 1227
SatI GCNGC 6 cut(s) 381, 576, 675, 678, 1570, 1640
Sau3AI GATC 3 cut(s) 321, 1470, 1646
Sau96I GGNCC 5 cut(s) 37, 505, 973, 1348, 1435
SchI GAGTC 1 cut(s) 1085
ScrFI CCNGG 1 cut(s) 20
SduI GDGCHC 2 cut(s) 480, 1369
SfaNI GCATC 5 cut(s) 393, 436, 931, 1212, 1669
SfcI CTRYAG 2 cut(s) 381, 1425
SinI GGWCC 3 cut(s) 37, 973, 1348
SmiMI CAYNNNNRTG 2 cut(s) 58, 111
Sse9I AATT 7 cut(s) 5, 68, 535, 811, 1476, 1602, 1652
SsiI CCGC 3 cut(s) 541, 623, 1569
SspMI CTAG 4 cut(s) 464, 516, 587, 1406
StyD4I CCNGG 1 cut(s) 18
StyI CCWWGG 1 cut(s) 481
TaaI ACNGT 6 cut(s) 93, 308, 347, 606, 934, 1587
TaqI TCGA 5 cut(s) 303, 741, 768, 869, 1206
TasI AATT 7 cut(s) 5, 68, 535, 811, 1476, 1602, 1652
TatI WGTACW 4 cut(s) 93, 649, 744, 1587
TauI GCSGC 1 cut(s) 1572
TfiI GAWTC 3 cut(s) 293, 512, 871
Tru1I TTAA 3 cut(s) 885, 1037, 1227
Tru9I TTAA 3 cut(s) 885, 1037, 1227
TscAI CASTG 2 cut(s) 445, 666
TseFI GTSAC 2 cut(s) 233, 424
TseI GCWGC 5 cut(s) 380, 575, 674, 677, 1639
Tsp45I GTSAC 2 cut(s) 233, 424
TspDTI ATGAA 9 cut(s) 129, 145, 821, 1229, 1317, 1567, 1607, 1716, 1727
TspGWI ACGGA 1 cut(s) 1403
TspRI CASTG 2 cut(s) 445, 666
VpaK11BI GGWCC 3 cut(s) 37, 973, 1348
XapI RAATTY 3 cut(s) 5, 68, 1476
XbaI TCTAGA 1 cut(s) 515
XceI RCATGY 2 cut(s) 241, 656
XcmI CCANNNNNNNNNTGG 2 cut(s) 799, 830
XspI CTAG 4 cut(s) 464, 516, 587, 1406
Zsp2I ATGCAT 3 cut(s) 451, 556, 1246
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.