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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AdeI | CACNNNGTG | 1 cut(s) | 104 |
| BccI | CCATC | 1 cut(s) | 5 |
| BclI | TGATCA | 1 cut(s) | 159 |
| BfaI | CTAG | 2 cut(s) | 116, 181 |
| BmsI | GCATC | 1 cut(s) | 115 |
| BseGI | GGATG | 1 cut(s) | 16 |
| Bsp143I | GATC | 1 cut(s) | 159 |
| BssMI | GATC | 1 cut(s) | 159 |
| Bst6I | CTCTTC | 1 cut(s) | 161 |
| BstF5I | GGATG | 1 cut(s) | 16 |
| BstKTI | GATC | 1 cut(s) | 162 |
| BstMBI | GATC | 1 cut(s) | 159 |
| BtsCI | GGATG | 1 cut(s) | 16 |
| CviAII | CATG | 1 cut(s) | 151 |
| CviJI | RGCY | 1 cut(s) | 140 |
| CviKI_1 | RGCY | 1 cut(s) | 140 |
| DpnI | GATC | 1 cut(s) | 161 |
| DpnII | GATC | 1 cut(s) | 159 |
| DraIII | CACNNNGTG | 1 cut(s) | 104 |
| Eam1104I | CTCTTC | 1 cut(s) | 161 |
| EarI | CTCTTC | 1 cut(s) | 161 |
| FaeI | CATG | 1 cut(s) | 154 |
| FaiI | YATR | 1 cut(s) | 152 |
| FatI | CATG | 1 cut(s) | 150 |
| FbaI | TGATCA | 1 cut(s) | 159 |
| FokI | GGATG | 1 cut(s) | 23 |
| FspBI | CTAG | 2 cut(s) | 116, 181 |
| Hin1II | CATG | 1 cut(s) | 154 |
| Hpy188III | TCNNGA | 1 cut(s) | 163 |
| HpyCH4V | TGCA | 1 cut(s) | 128 |
| Hsp92II | CATG | 1 cut(s) | 154 |
| Ksp22I | TGATCA | 1 cut(s) | 159 |
| Kzo9I | GATC | 1 cut(s) | 159 |
| LpnPI | CCDG | 1 cut(s) | 62 |
| LweI | GCATC | 1 cut(s) | 115 |
| MaeI | CTAG | 2 cut(s) | 116, 181 |
| MalI | GATC | 1 cut(s) | 161 |
| MboI | GATC | 1 cut(s) | 159 |
| MboII | GAAGA | 2 cut(s) | 17, 178 |
| MnlI | CCTC | 1 cut(s) | 162 |
| MseI | TTAA | 1 cut(s) | 39 |
| MslI | CAYNNNNRTG | 1 cut(s) | 41 |
| NdeII | GATC | 1 cut(s) | 159 |
| NlaIII | CATG | 1 cut(s) | 154 |
| RseI | CAYNNNNRTG | 1 cut(s) | 41 |
| SaqAI | TTAA | 1 cut(s) | 39 |
| Sau3AI | GATC | 1 cut(s) | 159 |
| SetI | ASST | 3 cut(s) | 45, 106, 173 |
| SfaNI | GCATC | 1 cut(s) | 115 |
| SgeI | CNNG | 6 cut(s) | 74, 89, 113, 128, 163, 175 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 41 |
| SspMI | CTAG | 2 cut(s) | 116, 181 |
| TaqI | TCGA | 1 cut(s) | 145 |
| Tru1I | TTAA | 1 cut(s) | 39 |
| Tru9I | TTAA | 1 cut(s) | 39 |
| XcmI | CCANNNNNNNNNTGG | 1 cut(s) | 148 |
| XspI | CTAG | 2 cut(s) | 116, 181 |