Rorug04G0045100

source UniProtKB

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000004
OG0000695
Physical Location & Seq
Forward (+)
7018377 .. 7021919
3543 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug04G0045100.1

Sequence Viewer

Length: 1089 bp
ATGGTCCTGGGAAAATCAAGCGACTCTGATCGTCTGGAGCAGCTGAAGGCCTTCGACGAATCCAAAGCTGGTGTCAAAGGGATCGTCGATGCCGGAATCACCAAAGTCCCACCCATTTTCGTCCGTCCAAAGAAAGATCCCACCACCGCTCTCAACCATAAGATATCTGGCCATTTCAGTATTCCAGTGGTTGATCTGGCTGACAATGAAGGAAGATCTGCCGAGGTGATCGACCAAGTCCGGAGGGCCGCAGAGAGCTACGGGTTGTTTCAGGTGGTGAACCATGGGATACCTGAGAGAGTGATGGAGGAGATGATGGAGGCGACGCATGGGTTTCACGAGCTTCCCAGGGAGGCCAAGGCTGAGTACTATAGTAGGGAGCTTGCAAGGAACGTCAAGTACTTCTCTGGCAGTTCCCTCTATATGTTGATGTTTGCTGATTGGAGGGACACTTTGCAGTGTAACATAAGTGACAAGCAGTTCGATCCTCAAGAAATTCCTCTCGTTTGCAGAGATATAATTATGGAGTATTCCAAGCATGCTCACAAATTAGGTGTCATTTTGTTTACATTGCTATCTGAGGCGCTTGGGCTCAAACCTGATCACCTCAAAAACATGGATTGCGCAGAGGAGCATGTGATTCTTAATCATTACTATCCCCCATGCCCGGAACCTGAACTAGCTATTGGTACTATCGAACACGCAGATCCCGATTTTCTGACAATCCTACTTCAAGACCAAATTGGTGGACTCCAGGTTTTGTACCAAAATCAGTGGATTGATGTTCCTCATGTACCTGGAGCTCTGGTGGTGAACATTGGAGATTTCTTGCAGCTAATGTCAAACAATAAGTTCATAAGCGTCAATCACAGAGTATTGGCTAAAAAGGAAGGTCCCAGAATTTCTGTGGCATGTTTTTTCAGACCATCACTAGAAAACTCTTCAAGATTGTATGGGCCAATCAAGGAGTTGACATCAGAAGAAAAACCTCCAGTTTACCGAGAAACAACTATGAAAGATTATACTCAGTGCCACTACAAACAATGGGAAGCAAATGAGGTATCCGGGCTTGACAGCTTGAAGCTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Peptidase_C48 Transposase_24

Protein Analysis

362

Amino Acids

41.01

Weight (kDa)

5.63

Isoelectric Point (pI)

41.05

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DIOX_N PF14226 61 - 164 1.6e-19 non-haem dioxygenase in morphine synthesis N-terminal
2OG-FeII_Oxy PF03171 211 - 309 1e-34 2OG-Fe(II) oxygenase superfamily
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 625
AccBSI CCGCTC 1 cut(s) 149
AccIII TCCGGA 1 cut(s) 240
AciI CCGC 2 cut(s) 147, 249
AclWI GGATC 4 cut(s) 89, 131, 479, 701
AcoI YGGCCR 1 cut(s) 169
AcsI RAATTY 2 cut(s) 495, 900
AcuI CTGAAG 1 cut(s) 65
AfaI GTAC 5 cut(s) 368, 401, 691, 764, 795
AfiI CCNNNNNNNGG 1 cut(s) 667
AgsI TTSAA 3 cut(s) 734, 945, 1081
AjnI CCWGG 4 cut(s) 6, 347, 753, 796
AjuI GAANNNNNNNTTGG 2 cut(s) 669, 701
Alw21I GWGCWC 1 cut(s) 805
AlwI GGATC 4 cut(s) 89, 131, 479, 701
Aor13HI TCCGGA 1 cut(s) 240
AoxI GGCC 5 cut(s) 48, 169, 246, 354, 956
ApeKI GCWGC 2 cut(s) 40, 832
ApoI RAATTY 2 cut(s) 495, 900
ArsI GACNNNNNNTTYG 8 cut(s) 57, 69, 89, 101, 464, 496, 540, 572
Asp700I GAANNNNTTC 1 cut(s) 50
AspLEI GCGC 2 cut(s) 586, 626
AspS9I GGNCC 4 cut(s) 4, 246, 893, 956
AsuC2I CCSGG 2 cut(s) 668, 1066
AsuHPI GGTGA 5 cut(s) 91, 238, 289, 596, 823
AvaII GGWCC 2 cut(s) 4, 893
BalI TGGCCA 1 cut(s) 171
BanII GRGCYC 2 cut(s) 594, 805
BauI CACGAG 1 cut(s) 338
Bbv12I GWGCWC 1 cut(s) 805
BbvI GCAGC 2 cut(s) 52, 844
BccI CCATC 3 cut(s) 298, 310, 934
BciT130I CCWGG 4 cut(s) 8, 349, 755, 798
BciVI GTATCC 2 cut(s) 282, 1072
BclI TGATCA 1 cut(s) 601
BcnI CCSGG 2 cut(s) 668, 1066
BfaI CTAG 2 cut(s) 680, 932
BfmI CTRYAG 1 cut(s) 370
BfoI RGCGCY 1 cut(s) 587
BfuI GTATCC 2 cut(s) 282, 1072
BglII AGATCT 1 cut(s) 215
BisI GCNGC 3 cut(s) 41, 249, 833
BlsI GCNGC 3 cut(s) 42, 250, 834
BmcAI AGTACT 2 cut(s) 368, 401
Bme1390I CCNGG 6 cut(s) 8, 349, 668, 755, 798, 1066
Bme18I GGWCC 2 cut(s) 4, 893
BmgT120I GGNCC 4 cut(s) 4, 246, 893, 956
BmiI GGNNCC 2 cut(s) 672, 895
BmrFI CCNGG 6 cut(s) 8, 349, 668, 755, 798, 1066
BmsI GCATC 1 cut(s) 79
BpmI CTGGAG 4 cut(s) 56, 737, 819, 975
BpuEI CTTGAG 1 cut(s) 474
BpuMI CCSGG 2 cut(s) 668, 1066
BsaJI CCNNGG 6 cut(s) 7, 222, 283, 347, 348, 357
BsaWI WCCGGW 1 cut(s) 240
BsaXI ACNNNNNCTCC 2 cut(s) 792, 822
Bsc4I CCNNNNNNNGG 1 cut(s) 667
Bse1I ACTGG 2 cut(s) 185, 992
Bse3DI GCAATG 1 cut(s) 569
BseAI TCCGGA 1 cut(s) 240
BseBI CCWGG 4 cut(s) 8, 349, 755, 798
BseDI CCNNGG 6 cut(s) 7, 222, 283, 347, 348, 357
BseLI CCNNNNNNNGG 1 cut(s) 667
BseMI GCAATG 1 cut(s) 569
BseMII CTCAG 4 cut(s) 285, 354, 570, 1040
BseNI ACTGG 2 cut(s) 185, 992
BseRI GAGGAG 2 cut(s) 323, 644
BseXI GCAGC 2 cut(s) 52, 844
BshFI GGCC 5 cut(s) 50, 171, 248, 356, 958
BsiHKAI GWGCWC 1 cut(s) 805
BsiSI CCGG 4 cut(s) 93, 241, 668, 1065
BslFI GGGAC 3 cut(s) 92, 461, 879
BslI CCNNNNNNNGG 1 cut(s) 667
BsmFI GGGAC 3 cut(s) 92, 461, 879
BsnI GGCC 5 cut(s) 50, 171, 248, 356, 958
Bsp1286I GDGCHC 2 cut(s) 594, 805
Bsp13I TCCGGA 1 cut(s) 240
Bsp143I GATC 9 cut(s) 28, 81, 136, 193, 215, 228, 484, 601, 706
Bsp19I CCATGG 1 cut(s) 283
BspACI CCGC 2 cut(s) 147, 249
BspANI GGCC 5 cut(s) 50, 171, 248, 356, 958
BspCNI CTCAG 4 cut(s) 286, 355, 571, 1039
BspEI TCCGGA 1 cut(s) 240
BspLI GGNNCC 2 cut(s) 672, 895
BspPI GGATC 4 cut(s) 89, 131, 479, 701
BsrBI CCGCTC 1 cut(s) 149
BsrDI GCAATG 1 cut(s) 569
BsrI ACTGG 2 cut(s) 185, 992
BssECI CCNNGG 6 cut(s) 7, 222, 283, 347, 348, 357
BssMI GATC 9 cut(s) 28, 81, 136, 193, 215, 228, 484, 601, 706
BssSI CACGAG 1 cut(s) 338
BssT1I CCWWGG 2 cut(s) 283, 357
Bst2BI CACGAG 1 cut(s) 338
Bst2UI CCWGG 4 cut(s) 8, 349, 755, 798
Bst6I CTCTTC 1 cut(s) 946
BstC8I GCNNGC 2 cut(s) 384, 540
BstDEI CTNAG 4 cut(s) 294, 363, 579, 1026
BstDSI CCRYGG 1 cut(s) 283
BstH2I RGCGCY 1 cut(s) 587
BstHHI GCGC 2 cut(s) 586, 626
BstKTI GATC 9 cut(s) 31, 84, 139, 196, 218, 231, 487, 604, 709
BstMBI GATC 9 cut(s) 28, 81, 136, 193, 215, 228, 484, 601, 706
BstNI CCWGG 4 cut(s) 8, 349, 755, 798
BstNSI RCATGY 3 cut(s) 542, 638, 915
BstSCI CCNGG 6 cut(s) 6, 347, 666, 753, 796, 1064
BstSFI CTRYAG 1 cut(s) 370
BstV1I GCAGC 2 cut(s) 52, 844
BstX2I RGATCY 3 cut(s) 136, 215, 706
BstXI CCANNNNNNTGG 1 cut(s) 746
BstYI RGATCY 3 cut(s) 136, 215, 706
BsuI GTATCC 2 cut(s) 282, 1072
BsuRI GGCC 5 cut(s) 50, 171, 248, 356, 958
BtgI CCRYGG 1 cut(s) 283
BtsI GCAGTG 1 cut(s) 464
BtsIMutI CAGTG 4 cut(s) 192, 464, 779, 1034
Cac8I GCNNGC 2 cut(s) 384, 540
CfoI GCGC 2 cut(s) 586, 626
Cfr13I GGNCC 4 cut(s) 4, 246, 893, 956
CseI GACGC 2 cut(s) 334, 850
Csp6I GTAC 5 cut(s) 367, 400, 690, 763, 794
CspCI CAANNNNNGTGG 2 cut(s) 755, 790
CviAII CATG 8 cut(s) 284, 329, 539, 616, 635, 663, 791, 912
CviQI GTAC 5 cut(s) 367, 400, 690, 763, 794
DdeI CTNAG 4 cut(s) 294, 363, 579, 1026
DpnI GATC 9 cut(s) 30, 83, 138, 195, 217, 230, 486, 603, 708
DpnII GATC 9 cut(s) 28, 81, 136, 193, 215, 228, 484, 601, 706
EaeI YGGCCR 1 cut(s) 169
Eam1104I CTCTTC 1 cut(s) 946
EarI CTCTTC 1 cut(s) 946
Ecl136II GAGCTC 1 cut(s) 803
Eco130I CCWWGG 2 cut(s) 283, 357
Eco147I AGGCCT 1 cut(s) 50
Eco24I GRGCYC 2 cut(s) 594, 805
Eco32I GATATC 1 cut(s) 165
Eco47I GGWCC 2 cut(s) 4, 893
Eco53kI GAGCTC 1 cut(s) 803
Eco57I CTGAAG 1 cut(s) 65
EcoICRI GAGCTC 1 cut(s) 803
EcoO109I RGGNCCY 1 cut(s) 893
EcoRII CCWGG 4 cut(s) 6, 347, 753, 796
EcoRV GATATC 1 cut(s) 165
EcoT14I CCWWGG 2 cut(s) 283, 357
EcoT38I GRGCYC 2 cut(s) 594, 805
ErhI CCWWGG 2 cut(s) 283, 357
FaeI CATG 8 cut(s) 287, 332, 542, 619, 638, 666, 794, 915
FaqI GGGAC 3 cut(s) 92, 461, 879
FatI CATG 8 cut(s) 283, 328, 538, 615, 634, 662, 790, 911
FbaI TGATCA 1 cut(s) 601
Fnu4HI GCNGC 3 cut(s) 41, 249, 833
FriOI GRGCYC 2 cut(s) 594, 805
Fsp4HI GCNGC 3 cut(s) 41, 249, 833
FspBI CTAG 2 cut(s) 680, 932
FspI TGCGCA 1 cut(s) 625
GlaI GCGC 2 cut(s) 585, 625
GluI GCNGC 3 cut(s) 41, 249, 833
GsuI CTGGAG 4 cut(s) 56, 737, 819, 975
HaeII RGCGCY 1 cut(s) 587
HaeIII GGCC 5 cut(s) 50, 171, 248, 356, 958
HapII CCGG 4 cut(s) 93, 241, 668, 1065
HgaI GACGC 2 cut(s) 334, 850
HhaI GCGC 2 cut(s) 586, 626
Hin1II CATG 8 cut(s) 287, 332, 542, 619, 638, 666, 794, 915
Hin6I GCGC 2 cut(s) 584, 624
HinP1I GCGC 2 cut(s) 584, 624
HincII GTYRAC 1 cut(s) 972
HindII GTYRAC 1 cut(s) 972
HinfI GANTC 5 cut(s) 23, 59, 96, 640, 750
HpaII CCGG 4 cut(s) 93, 241, 668, 1065
HphI GGTGA 5 cut(s) 91, 238, 289, 596, 823
Hpy166II GTNNAC 6 cut(s) 280, 567, 749, 814, 972, 997
Hpy188I TCNGA 5 cut(s) 28, 580, 720, 923, 979
Hpy188III TCNNGA 7 cut(s) 35, 241, 338, 491, 710, 734, 945
Hpy8I GTNNAC 6 cut(s) 280, 567, 749, 814, 972, 997
Hpy99I CGWCG 3 cut(s) 59, 89, 328
HpyAV CCTTC 4 cut(s) 40, 61, 203, 884
HpyCH4IV ACGT 1 cut(s) 393
HpyCH4V TGCA 4 cut(s) 386, 457, 510, 832
HpyF3I CTNAG 4 cut(s) 294, 363, 579, 1026
HpySE526I ACGT 1 cut(s) 393
Hsp92II CATG 8 cut(s) 287, 332, 542, 619, 638, 666, 794, 915
HspAI GCGC 2 cut(s) 584, 624
Kpn2I TCCGGA 1 cut(s) 240
Ksp22I TGATCA 1 cut(s) 601
Kzo9I GATC 9 cut(s) 28, 81, 136, 193, 215, 228, 484, 601, 706
LmnI GCTCC 4 cut(s) 37, 379, 631, 800
Lsp1109I GCAGC 2 cut(s) 52, 844
LweI GCATC 1 cut(s) 79
MaeI CTAG 2 cut(s) 680, 932
MaeII ACGT 1 cut(s) 393
MaeIII GTNAC 2 cut(s) 461, 470
MalI GATC 9 cut(s) 30, 83, 138, 195, 217, 230, 486, 603, 708
MbiI CCGCTC 1 cut(s) 149
MboI GATC 9 cut(s) 28, 81, 136, 193, 215, 228, 484, 601, 706
MboII GAAGA 3 cut(s) 225, 933, 992
MflI RGATCY 3 cut(s) 136, 215, 706
MhlI GDGCHC 2 cut(s) 594, 805
MlsI TGGCCA 1 cut(s) 171
MluCI AATT 5 cut(s) 495, 519, 548, 741, 900
MluNI TGGCCA 1 cut(s) 171
MlyI GAGTC 2 cut(s) 17, 744
Mox20I TGGCCA 1 cut(s) 171
MroI TCCGGA 1 cut(s) 240
MroXI GAANNNNTTC 1 cut(s) 50
MscI TGGCCA 1 cut(s) 171
MseI TTAA 1 cut(s) 645
Msp20I TGGCCA 1 cut(s) 171
MspA1I CMGCKG 1 cut(s) 43
MspI CCGG 4 cut(s) 93, 241, 668, 1065
MspR9I CCNGG 6 cut(s) 8, 349, 668, 755, 798, 1066
MvaI CCWGG 4 cut(s) 8, 349, 755, 798
NciI CCSGG 2 cut(s) 668, 1066
NcoI CCATGG 1 cut(s) 283
NdeII GATC 9 cut(s) 28, 81, 136, 193, 215, 228, 484, 601, 706
NlaIII CATG 8 cut(s) 287, 332, 542, 619, 638, 666, 794, 915
NlaIV GGNNCC 2 cut(s) 672, 895
NmeAIII GCCGAG 1 cut(s) 247
NmuCI GTSAC 1 cut(s) 470
NsbI TGCGCA 1 cut(s) 625
NspI RCATGY 3 cut(s) 542, 638, 915
PaeI GCATGC 1 cut(s) 542
PasI CCCWGGG 1 cut(s) 348
PceI AGGCCT 1 cut(s) 50
PcsI WCGNNNNNNNCGW 1 cut(s) 708
PdmI GAANNNNTTC 1 cut(s) 50
PfeI GAWTC 3 cut(s) 59, 96, 640
PflFI GACNNNGTC 1 cut(s) 236
PkrI GCNGC 3 cut(s) 42, 250, 834
PleI GAGTC 2 cut(s) 17, 744
PpsI GAGTC 2 cut(s) 17, 744
PpuMI RGGWCCY 1 cut(s) 893
Psp124BI GAGCTC 1 cut(s) 805
Psp5II RGGWCCY 1 cut(s) 893
Psp6I CCWGG 4 cut(s) 6, 347, 753, 796
PspGI CCWGG 4 cut(s) 6, 347, 753, 796
PspN4I GGNNCC 2 cut(s) 672, 895
PspPI GGNCC 4 cut(s) 4, 246, 893, 956
PspPPI RGGWCCY 1 cut(s) 893
PsrI GAACNNNNNNTAC 2 cut(s) 383, 415
PsuI RGATCY 3 cut(s) 136, 215, 706
PsyI GACNNNGTC 1 cut(s) 236
PvuII CAGCTG 1 cut(s) 43
RsaI GTAC 5 cut(s) 368, 401, 691, 764, 795
RsaNI GTAC 5 cut(s) 367, 400, 690, 763, 794
SacI GAGCTC 1 cut(s) 805
SaqAI TTAA 1 cut(s) 645
SatI GCNGC 3 cut(s) 41, 249, 833
Sau3AI GATC 9 cut(s) 28, 81, 136, 193, 215, 228, 484, 601, 706
Sau96I GGNCC 4 cut(s) 4, 246, 893, 956
ScaI AGTACT 2 cut(s) 368, 401
SchI GAGTC 2 cut(s) 17, 744
ScrFI CCNGG 6 cut(s) 8, 349, 668, 755, 798, 1066
SduI GDGCHC 2 cut(s) 594, 805
SfaNI GCATC 1 cut(s) 79
SfcI CTRYAG 1 cut(s) 370
SinI GGWCC 2 cut(s) 4, 893
SmlI CTYRAG 1 cut(s) 489
SmoI CTYRAG 1 cut(s) 489
SphI GCATGC 1 cut(s) 542
Sse9I AATT 5 cut(s) 495, 519, 548, 741, 900
SseBI AGGCCT 1 cut(s) 50
SsiI CCGC 2 cut(s) 147, 249
SspMI CTAG 2 cut(s) 680, 932
SstI GAGCTC 1 cut(s) 805
StuI AGGCCT 1 cut(s) 50
StyD4I CCNGG 6 cut(s) 6, 347, 666, 753, 796, 1064
StyI CCWWGG 2 cut(s) 283, 357
TaiI ACGT 1 cut(s) 396
TaqI TCGA 5 cut(s) 54, 87, 231, 483, 696
TasI AATT 5 cut(s) 495, 519, 548, 741, 900
TatI WGTACW 2 cut(s) 366, 399
TauI GCSGC 1 cut(s) 251
TfiI GAWTC 3 cut(s) 59, 96, 640
Tru1I TTAA 1 cut(s) 645
Tru9I TTAA 1 cut(s) 645
TscAI CASTG 4 cut(s) 192, 464, 779, 1034
TseFI GTSAC 1 cut(s) 470
TseI GCWGC 2 cut(s) 40, 832
Tsp45I GTSAC 1 cut(s) 470
TspDTI ATGAA 3 cut(s) 222, 844, 1028
TspGWI ACGGA 1 cut(s) 113
TspRI CASTG 4 cut(s) 192, 464, 779, 1034
Tth111I GACNNNGTC 1 cut(s) 236
VpaK11BI GGWCC 2 cut(s) 4, 893
XapI RAATTY 2 cut(s) 495, 900
XceI RCATGY 3 cut(s) 542, 638, 915
XcmI CCANNNNNNNNNTGG 2 cut(s) 164, 904
XmnI GAANNNNTTC 1 cut(s) 50
XspI CTAG 2 cut(s) 680, 932
ZrmI AGTACT 2 cut(s) 368, 401
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.