Rroxscaffold_4G00327440

tRNA-specific 2-thiouridylase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000004
OG0000695
Physical Location & Seq
Reverse (-)
60152886 .. 60157734
4849 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_4G00327440.1

Sequence Viewer

Length: 588 bp
ATGAGATTGGAAGCAAAATTAGATGGGAGAGCCCTTCCCATTGAGTCGCCTCCCGAGGCCATGTTACCAAGGATCTTGGTTACGGGACAAGGTAGCTGCTCCAATTTGCGGGAGAAGTGTCCTCTTCAAAAGGTCGAAGACCTTGTTGTCGGCGCAAAGAAGCGTTTGGAGTTAGAGGTTGAAGTTCGGGAGAAAAAACAACGGCCTAAGTACGGTAAGGGTCCGAAGAAGAGGAAAAGGCCGGTAGAGGAGGATGAAGAGGAGGATCTCGACTTGAAGAAACTGCCAAAAGATTACCCTGCACCGTTGAAATGCTTGTGGTTGTGGGCTAGAGACAATTTGGCAAATGGGAGGACAATGTCCTTTCGGATGGAAGATAAAGTTTTTGGCATCAACGGGAAGCATTATTTGTTTAAGGGAGACATTTATGCATTGTGTACCATGTCTGAACTATACGGTGGAGTGATCTCCATGTATATTCATGGCATTGAAGATGTAGCAGTTGTTAACTTATCTGGAGATGATCAAGGCCTGGTAGCGGGGCAGTTTGTGGCTTTCTACTGGGGAAGAACCTGCTTACTTGCTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00561 ko00564 ko01100 ko01110 ko04072 map00561 map00564 map01100 map01110 map04072
Pfam Domains
Protein Families
tRNA_Me_trans

Protein Analysis

195

Amino Acids

22.09

Weight (kDa)

8.51

Isoelectric Point (pI)

56.31

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 146
Acc36I ACCTGC 1 cut(s) 581
AciI CCGC 2 cut(s) 109, 539
AclWI GGATC 2 cut(s) 80, 273
AfaI GTAC 2 cut(s) 212, 439
AfiI CCNNNNNNNGG 3 cut(s) 108, 212, 538
AgsI TTSAA 5 cut(s) 128, 182, 277, 310, 491
AjnI CCWGG 1 cut(s) 531
AluBI AGCT 1 cut(s) 96
AluI AGCT 1 cut(s) 96
Alw26I GTCTC 2 cut(s) 327, 414
AlwI GGATC 2 cut(s) 80, 273
Ama87I CYCGRG 1 cut(s) 53
AoxI GGCC 4 cut(s) 57, 203, 239, 529
ApeKI GCWGC 1 cut(s) 96
AspLEI GCGC 1 cut(s) 155
AspS9I GGNCC 1 cut(s) 221
AvaI CYCGRG 1 cut(s) 53
AvaII GGWCC 1 cut(s) 221
BanII GRGCYC 1 cut(s) 34
BbsI GAAGAC 1 cut(s) 144
BbvI GCAGC 1 cut(s) 83
BccI CCATC 2 cut(s) 17, 364
BceAI ACGGC 1 cut(s) 218
BciT130I CCWGG 1 cut(s) 533
BclI TGATCA 1 cut(s) 523
BcoDI GTCTC 2 cut(s) 327, 414
BfaI CTAG 1 cut(s) 330
BfuAI ACCTGC 1 cut(s) 581
BisI GCNGC 1 cut(s) 97
BlsI GCNGC 1 cut(s) 98
Bme1390I CCNGG 1 cut(s) 533
Bme18I GGWCC 1 cut(s) 221
BmeT110I CYCGRG 1 cut(s) 53
BmgT120I GGNCC 1 cut(s) 221
BmiI GGNNCC 1 cut(s) 222
BmrFI CCNGG 1 cut(s) 533
BmrI ACTGGG 1 cut(s) 571
BmsI GCATC 1 cut(s) 399
BmuI ACTGGG 1 cut(s) 571
BpiI GAAGAC 1 cut(s) 144
BpmI CTGGAG 1 cut(s) 537
BsaJI CCNNGG 2 cut(s) 54, 68
Bsc4I CCNNNNNNNGG 3 cut(s) 108, 212, 538
Bse118I RCCGGY 1 cut(s) 241
Bse1I ACTGG 1 cut(s) 566
BseBI CCWGG 1 cut(s) 533
BseDI CCNNGG 2 cut(s) 54, 68
BseGI GGATG 2 cut(s) 259, 375
BseLI CCNNNNNNNGG 3 cut(s) 108, 212, 538
BseNI ACTGG 1 cut(s) 566
BseRI GAGGAG 2 cut(s) 263, 275
BseXI GCAGC 1 cut(s) 83
BsgI GTGCAG 1 cut(s) 285
BshFI GGCC 4 cut(s) 59, 205, 241, 531
BsiHKCI CYCGRG 1 cut(s) 53
BsiSI CCGG 1 cut(s) 242
BslFI GGGAC 1 cut(s) 99
BslI CCNNNNNNNGG 3 cut(s) 108, 212, 538
BsmAI GTCTC 2 cut(s) 327, 414
BsmFI GGGAC 1 cut(s) 99
BsnI GGCC 4 cut(s) 59, 205, 241, 531
BsoBI CYCGRG 1 cut(s) 53
Bsp1286I GDGCHC 1 cut(s) 34
Bsp143I GATC 4 cut(s) 72, 265, 465, 523
BspACI CCGC 2 cut(s) 109, 539
BspANI GGCC 4 cut(s) 59, 205, 241, 531
BspLI GGNNCC 1 cut(s) 222
BspMI ACCTGC 1 cut(s) 581
BspPI GGATC 2 cut(s) 80, 273
BsrFI RCCGGY 1 cut(s) 241
BsrI ACTGG 1 cut(s) 566
BssAI RCCGGY 1 cut(s) 241
BssECI CCNNGG 2 cut(s) 54, 68
BssMI GATC 4 cut(s) 72, 265, 465, 523
BssT1I CCWWGG 1 cut(s) 68
Bst2UI CCWGG 1 cut(s) 533
Bst4CI ACNGT 3 cut(s) 215, 306, 458
Bst6I CTCTTC 3 cut(s) 129, 224, 252
BstDEI CTNAG 2 cut(s) 207, 585
BstF5I GGATG 2 cut(s) 259, 375
BstHHI GCGC 1 cut(s) 155
BstKTI GATC 4 cut(s) 75, 268, 468, 526
BstMAI GTCTC 2 cut(s) 327, 414
BstMBI GATC 4 cut(s) 72, 265, 465, 523
BstNI CCWGG 1 cut(s) 533
BstSCI CCNGG 1 cut(s) 531
BstV1I GCAGC 1 cut(s) 83
BstV2I GAAGAC 1 cut(s) 144
BstX2I RGATCY 2 cut(s) 72, 265
BstYI RGATCY 2 cut(s) 72, 265
BsuRI GGCC 4 cut(s) 59, 205, 241, 531
BtsCI GGATG 2 cut(s) 259, 375
BveI ACCTGC 1 cut(s) 581
CfoI GCGC 1 cut(s) 155
Cfr10I RCCGGY 1 cut(s) 241
Cfr13I GGNCC 1 cut(s) 221
Csp6I GTAC 2 cut(s) 211, 438
CviAII CATG 4 cut(s) 61, 442, 472, 482
CviJI RGCY 8 cut(s) 32, 59, 96, 205, 241, 329, 531, 554
CviKI_1 RGCY 8 cut(s) 32, 59, 96, 205, 241, 329, 531, 554
CviQI GTAC 2 cut(s) 211, 438
DdeI CTNAG 2 cut(s) 207, 585
DpnI GATC 4 cut(s) 74, 267, 467, 525
DpnII GATC 4 cut(s) 72, 265, 465, 523
DrdI GACNNNNNNGTC 1 cut(s) 146
DseDI GACNNNNNNGTC 1 cut(s) 146
Eam1104I CTCTTC 3 cut(s) 129, 224, 252
EarI CTCTTC 3 cut(s) 129, 224, 252
Eco130I CCWWGG 1 cut(s) 68
Eco147I AGGCCT 1 cut(s) 531
Eco24I GRGCYC 1 cut(s) 34
Eco47I GGWCC 1 cut(s) 221
Eco88I CYCGRG 1 cut(s) 53
EcoRII CCWGG 1 cut(s) 531
EcoT14I CCWWGG 1 cut(s) 68
EcoT22I ATGCAT 1 cut(s) 433
EcoT38I GRGCYC 1 cut(s) 34
ErhI CCWWGG 1 cut(s) 68
FaeI CATG 4 cut(s) 64, 445, 475, 485
FaiI YATR 7 cut(s) 62, 429, 443, 454, 473, 477, 483
FaqI GGGAC 1 cut(s) 99
FatI CATG 4 cut(s) 60, 441, 471, 481
FauI CCCGC 2 cut(s) 102, 532
FbaI TGATCA 1 cut(s) 523
Fnu4HI GCNGC 1 cut(s) 97
FokI GGATG 2 cut(s) 266, 382
FriOI GRGCYC 1 cut(s) 34
Fsp4HI GCNGC 1 cut(s) 97
FspBI CTAG 1 cut(s) 330
GlaI GCGC 1 cut(s) 154
GluI GCNGC 1 cut(s) 97
GsuI CTGGAG 1 cut(s) 537
HaeIII GGCC 4 cut(s) 59, 205, 241, 531
HapII CCGG 1 cut(s) 242
HhaI GCGC 1 cut(s) 155
Hin1II CATG 4 cut(s) 64, 445, 475, 485
Hin6I GCGC 1 cut(s) 153
HinP1I GCGC 1 cut(s) 153
HincII GTYRAC 1 cut(s) 508
HindII GTYRAC 1 cut(s) 508
HinfI GANTC 1 cut(s) 44
HpaI GTTAAC 1 cut(s) 508
HpaII CCGG 1 cut(s) 242
Hpy166II GTNNAC 2 cut(s) 438, 508
Hpy188I TCNGA 3 cut(s) 225, 369, 448
Hpy188III TCNNGA 4 cut(s) 53, 188, 269, 516
Hpy8I GTNNAC 2 cut(s) 438, 508
HpyAV CCTTC 1 cut(s) 44
HpyCH4III ACNGT 3 cut(s) 215, 306, 458
HpyCH4V TGCA 2 cut(s) 302, 431
HpyF3I CTNAG 2 cut(s) 207, 585
Hsp92II CATG 4 cut(s) 64, 445, 475, 485
HspAI GCGC 1 cut(s) 153
Ksp22I TGATCA 1 cut(s) 523
KspAI GTTAAC 1 cut(s) 508
Kzo9I GATC 4 cut(s) 72, 265, 465, 523
LmnI GCTCC 1 cut(s) 104
LpnPI CCDG 6 cut(s) 255, 312, 501, 518, 545, 547
Lsp1109I GCAGC 1 cut(s) 83
LweI GCATC 1 cut(s) 399
MaeI CTAG 1 cut(s) 330
MaeIII GTNAC 2 cut(s) 63, 79
MalI GATC 4 cut(s) 74, 267, 467, 525
MboI GATC 4 cut(s) 72, 265, 465, 523
MboII GAAGA 9 cut(s) 116, 149, 238, 241, 269, 289, 386, 503, 579
MflI RGATCY 2 cut(s) 72, 265
MhlI GDGCHC 1 cut(s) 34
MluCI AATT 3 cut(s) 17, 103, 337
MlyI GAGTC 1 cut(s) 53
Mph1103I ATGCAT 1 cut(s) 433
MseI TTAA 2 cut(s) 414, 507
MspI CCGG 1 cut(s) 242
MspR9I CCNGG 1 cut(s) 533
MvaI CCWGG 1 cut(s) 533
NdeII GATC 4 cut(s) 72, 265, 465, 523
NlaIII CATG 4 cut(s) 64, 445, 475, 485
NlaIV GGNNCC 1 cut(s) 222
NsiI ATGCAT 1 cut(s) 433
PceI AGGCCT 1 cut(s) 531
PflFI GACNNNGTC 1 cut(s) 358
PkrI GCNGC 1 cut(s) 98
PleI GAGTC 1 cut(s) 52
PpsI GAGTC 1 cut(s) 52
Psp6I CCWGG 1 cut(s) 531
PspGI CCWGG 1 cut(s) 531
PspN4I GGNNCC 1 cut(s) 222
PspPI GGNCC 1 cut(s) 221
PsuI RGATCY 2 cut(s) 72, 265
PsyI GACNNNGTC 1 cut(s) 358
RsaI GTAC 2 cut(s) 212, 439
RsaNI GTAC 2 cut(s) 211, 438
SaqAI TTAA 2 cut(s) 414, 507
SatI GCNGC 1 cut(s) 97
Sau3AI GATC 4 cut(s) 72, 265, 465, 523
Sau96I GGNCC 1 cut(s) 221
SchI GAGTC 1 cut(s) 53
ScrFI CCNGG 1 cut(s) 533
SduI GDGCHC 1 cut(s) 34
SetI ASST 6 cut(s) 94, 98, 135, 144, 180, 575
SfaNI GCATC 1 cut(s) 399
SinI GGWCC 1 cut(s) 221
Sse9I AATT 3 cut(s) 17, 103, 337
SseBI AGGCCT 1 cut(s) 531
SsiI CCGC 2 cut(s) 109, 539
SspMI CTAG 1 cut(s) 330
StuI AGGCCT 1 cut(s) 531
StyD4I CCNGG 1 cut(s) 531
StyI CCWWGG 1 cut(s) 68
TaaI ACNGT 3 cut(s) 215, 306, 458
TaqI TCGA 2 cut(s) 135, 270
TasI AATT 3 cut(s) 17, 103, 337
Tru1I TTAA 2 cut(s) 414, 507
Tru9I TTAA 2 cut(s) 414, 507
TseI GCWGC 1 cut(s) 96
TspDTI ATGAA 2 cut(s) 270, 470
Tth111I GACNNNGTC 1 cut(s) 358
VpaK11BI GGWCC 1 cut(s) 221
XspI CTAG 1 cut(s) 330
Zsp2I ATGCAT 1 cut(s) 433
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.