Rmu_sc0006244.1_g000030

source UniProtKB

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0006244.1
OG0000695
Physical Location & Seq
Reverse (-)
108373 .. 112096
3724 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0006244.1_g000030.1.cds

Sequence Viewer

Length: 2085 bp
atggctccttcaaaatctagggctgccactagagcaaggcagaatattatcaatgcattgaaagtgacaagagcgagatctgcaccagaactagcatcttctgccgtgtcaaatgcatcagaaaacaagccatcaccaaagagagcaacgacatcgctaagggtcagcaagctattgacgaagcataagaagaaaaagtcatctaaggcatccagcccagaaaaaagcggcttaaagttgctgaagtgtggttgtgttacaatgcacaggattgtgagaaggaaaattcttggggtaaagttgaaagtcttgtttaatcagaagggatcgccttatggtgctgcatccaaggaaatgcagtcatatattggggtactagcatgtactaaggcaccaatatgcagaccttcttggaagcaggtcccaaaggatcgtaaaaataagatttggtggtgtgtcgagatggcatttgaggtacctccggaggcaaggagaatggtcctatcatcagcttcacagaagtggagagaatttaagagcaagctaactacacagtacatcatcccacataaggatgaacctgaattgttagagtatcccccagctgattacaatttcatcgagaagtctcattgggacatctttgtaactgatcgattgtctgaggaatttcagtctgaaatcaatcctgattcggaaatcgaccgtgcaacaatgtggatcaaggcacggcaggataaacatggcaacttcaaagatgttgaggtagagaagtgtgccgcagacatagaaaagctgaagaggcaagtcagtgacggtgagatgacatcttgtggcactgatgatgtattgacaaaggctctaggtaaccctaaacaccatggaagagtgcgtggtgtggggggtcatgtcaagcctggcgtatattttaacctaccgaggcaaaaaaggaaaactgttgaagagagaattaaggaagactgcaagaaattaatagaggaggaggcagagaaaattgtgtcacgagagagagcacattgggcagaaacaataatgaggttggaagcaaaacttgaagggaaaacctttgccattgagtcccctacggcaggccagaaggaccttggttctggacaaggtagttgctccactttgcgggagaagtgtgcatttcaaaagattgaggacgctgccggtatctttgcaaagaagcggttagacttagtggatgacattgtgaaggaagtggaagaagataaggttgtacaggttccggtggaagaactagctgctgcagtagttcatgagaaggctgttgagttggttgaagttacacaacaggaacttaaagagttcgagtgcagattggctttagggtcgatcgacaatattgttgctattgttaccattgttgaagtggacagtaatggcgaaaaccagacacttcatggtgttgctttggaagaagggaatgtgcgagtttcgattctgcgtccacttgatcctcaagctaaggttccattcccagtcaacgatgagatagttactgtcaaagatgctattgggacttacattgcttggccgagggacctcatacttcaatcccctcccgacactatgaagaaacaaccaaagaagtccagtaagggtttgaagaagaggaaaaggcaagtaggcattcctgaagaggagaatattgacatgaagaagctaccaaaagattaccctgcacctttgaagtgcttgtggatttgggcaagggacaatttggcaagtgggagaacaatctccttcaatttggaacataaagtgtttggcatcgacatgaagcattatctgtttaagtcagacattcatgcattgtgtacaatgactgaactatcaggtggaggtattccggcacaacaaaatgtgaaggactgtggcatattcatcatgcggtacatgaaggaaataattgaggataagaacctgggttttgcttcaaagtggcagcgacgatccgaattgatctacacccaaaaggatgttgatgttgtgaggtcagaatgggctaagtttgtgatgaagtatcatatttcatag
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Peptidase_C48 Transposase_24

Protein Analysis

694

Amino Acids

78.48

Weight (kDa)

9.29

Isoelectric Point (pI)

48.53

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 409
Acc65I GGTACC 1 cut(s) 475
AccB1I GGYRCC 2 cut(s) 391, 475
AccIII TCCGGA 1 cut(s) 481
AciI CCGC 5 cut(s) 228, 780, 1156, 1213, 1939
AclWI GGATC 5 cut(s) 334, 438, 728, 1496, 1995
AcoI YGGCCR 1 cut(s) 1580
AcsI RAATTY 3 cut(s) 285, 530, 668
AcuI CTGAAG 3 cut(s) 263, 818, 1704
AfaI GTAC 7 cut(s) 375, 385, 477, 557, 1266, 1867, 1943
AfiI CCNNNNNNNGG 3 cut(s) 571, 1109, 1155
AjnI CCWGG 2 cut(s) 916, 1971
AleI CACNNNNGTG 1 cut(s) 520
AluBI AGCT 8 cut(s) 172, 512, 544, 605, 796, 1289, 1511, 1711
AluI AGCT 8 cut(s) 172, 512, 544, 605, 796, 1289, 1511, 1711
Alw21I GWGCWC 1 cut(s) 1036
Alw26I GTCTC 1 cut(s) 633
AlwI GGATC 5 cut(s) 334, 438, 728, 1496, 1995
Aor13HI TCCGGA 1 cut(s) 481
AoxI GGCC 2 cut(s) 1111, 1580
ApeKI GCWGC 6 cut(s) 23, 341, 1190, 1289, 1292, 1993
ApoI RAATTY 3 cut(s) 285, 530, 668
AseI ATTAAT 1 cut(s) 992
Asp718I GGTACC 1 cut(s) 475
AspS9I GGNCC 4 cut(s) 421, 499, 1120, 1588
AsuHPI GGTGA 2 cut(s) 126, 830
AvaII GGWCC 4 cut(s) 421, 499, 1120, 1588
BanI GGYRCC 2 cut(s) 391, 475
BauI CACGAG 1 cut(s) 1023
BbsI GAAGAC 1 cut(s) 984
Bbv12I GWGCWC 1 cut(s) 1036
BbvI GCAGC 6 cut(s) 10, 328, 1177, 1276, 1279, 2005
BccI CCATC 2 cut(s) 139, 457
BceAI ACGGC 3 cut(s) 89, 746, 1122
BcgI CGANNNNNNTGC 2 cut(s) 135, 169
BciT130I CCWGG 2 cut(s) 918, 1973
BciVI GTATCC 1 cut(s) 606
BcoDI GTCTC 1 cut(s) 633
BfaI CTAG 6 cut(s) 18, 30, 92, 377, 863, 1286
BfmI CTRYAG 1 cut(s) 1293
BfuAI ACCTGC 1 cut(s) 409
BfuI GTATCC 1 cut(s) 606
BglII AGATCT 1 cut(s) 77
BisI GCNGC 8 cut(s) 24, 229, 342, 780, 1191, 1290, 1293, 1994
BlsI GCNGC 8 cut(s) 25, 230, 343, 781, 1192, 1291, 1294, 1995
Bme1390I CCNGG 2 cut(s) 918, 1973
Bme18I GGWCC 4 cut(s) 421, 499, 1120, 1588
BmgT120I GGNCC 4 cut(s) 421, 499, 1120, 1588
BmiI GGNNCC 7 cut(s) 6, 393, 423, 477, 1272, 1518, 1589
BmrFI CCNGG 2 cut(s) 918, 1973
BmrI ACTGGG 1 cut(s) 1520
BmsI GCATC 6 cut(s) 104, 125, 218, 353, 1546, 1827
BmuI ACTGGG 1 cut(s) 1520
BpiI GAAGAC 1 cut(s) 984
Bpu10I CCTNAGC 2 cut(s) 158, 1512
BpuEI CTTGAG 1 cut(s) 1491
Bsa29I ATCGAT 1 cut(s) 655
BsaJI CCNNGG 6 cut(s) 348, 880, 938, 1123, 1583, 1972
BsaWI WCCGGW 2 cut(s) 481, 1273
Bsc4I CCNNNNNNNGG 3 cut(s) 571, 1109, 1155
Bse118I RCCGGY 1 cut(s) 1193
Bse1I ACTGG 2 cut(s) 1526, 1641
Bse3DI GCAATG 1 cut(s) 1572
BseAI TCCGGA 1 cut(s) 481
BseBI CCWGG 2 cut(s) 918, 1973
BseCI ATCGAT 1 cut(s) 655
BseDI CCNNGG 6 cut(s) 348, 880, 938, 1123, 1583, 1972
BseGI GGATG 6 cut(s) 209, 344, 561, 580, 1234, 2032
BseLI CCNNNNNNNGG 3 cut(s) 571, 1109, 1155
BseMI GCAATG 1 cut(s) 1572
BseMII CTCAG 1 cut(s) 654
BseNI ACTGG 2 cut(s) 1526, 1641
BseRI GAGGAG 3 cut(s) 1013, 1016, 1703
BseXI GCAGC 6 cut(s) 10, 328, 1177, 1276, 1279, 2005
BseYI CCCAGC 1 cut(s) 601
BsgI GTGCAG 3 cut(s) 66, 1381, 1713
Bsh1285I CGRYCG 2 cut(s) 706, 1383
BshFI GGCC 2 cut(s) 1113, 1582
BshNI GGYRCC 2 cut(s) 391, 475
BshVI ATCGAT 1 cut(s) 655
BsiEI CGRYCG 2 cut(s) 706, 1383
BsiHKAI GWGCWC 1 cut(s) 1036
BsiSI CCGG 4 cut(s) 482, 1194, 1274, 1898
BslFI GGGAC 6 cut(s) 407, 650, 1084, 1579, 1601, 1775
BslI CCNNNNNNNGG 3 cut(s) 571, 1109, 1155
BsmAI GTCTC 1 cut(s) 633
BsmFI GGGAC 6 cut(s) 407, 650, 1084, 1579, 1601, 1775
BsmI GAATGC 1 cut(s) 1677
BsnI GGCC 2 cut(s) 1113, 1582
Bsp1286I GDGCHC 1 cut(s) 1036
Bsp13I TCCGGA 1 cut(s) 481
Bsp1407I TGTACA 2 cut(s) 1264, 1865
Bsp143I GATC 9 cut(s) 77, 326, 430, 652, 720, 1380, 1501, 2000, 2010
Bsp19I CCATGG 1 cut(s) 880
BspACI CCGC 5 cut(s) 228, 780, 1156, 1213, 1939
BspANI GGCC 2 cut(s) 1113, 1582
BspCNI CTCAG 1 cut(s) 655
BspDI ATCGAT 1 cut(s) 655
BspEI TCCGGA 1 cut(s) 481
BspHI TCATGA 1 cut(s) 1303
BspLI GGNNCC 7 cut(s) 6, 393, 423, 477, 1272, 1518, 1589
BspMAI CTGCAG 1 cut(s) 1297
BspMI ACCTGC 1 cut(s) 409
BspPI GGATC 5 cut(s) 334, 438, 728, 1496, 1995
BspT107I GGYRCC 2 cut(s) 391, 475
BsrDI GCAATG 1 cut(s) 1572
BsrFI RCCGGY 1 cut(s) 1193
BsrGI TGTACA 2 cut(s) 1264, 1865
BsrI ACTGG 2 cut(s) 1526, 1641
BssAI RCCGGY 1 cut(s) 1193
BssECI CCNNGG 6 cut(s) 348, 880, 938, 1123, 1583, 1972
BssMI GATC 9 cut(s) 77, 326, 430, 652, 720, 1380, 1501, 2000, 2010
BssSI CACGAG 1 cut(s) 1023
BssT1I CCWWGG 3 cut(s) 348, 880, 1123
Bst2BI CACGAG 1 cut(s) 1023
Bst2UI CCWGG 2 cut(s) 918, 1973
Bst4CI ACNGT 7 cut(s) 555, 707, 818, 958, 1424, 1549, 1922
Bst6I CTCTTC 5 cut(s) 794, 880, 957, 1652, 1680
BstAPI GCANNNNNTGC 1 cut(s) 101
BstAUI TGTACA 2 cut(s) 1264, 1865
BstC8I GCNNGC 3 cut(s) 170, 542, 1111
BstDEI CTNAG 7 cut(s) 158, 204, 387, 663, 1222, 1512, 2055
BstDSI CCRYGG 1 cut(s) 880
BstEII GGTNACC 1 cut(s) 866
BstENI CCTNNNNNAGG 1 cut(s) 1107
BstF5I GGATG 6 cut(s) 209, 344, 561, 580, 1234, 2032
BstKTI GATC 9 cut(s) 80, 329, 433, 655, 723, 1383, 1504, 2003, 2013
BstMAI GTCTC 1 cut(s) 633
BstMBI GATC 9 cut(s) 77, 326, 430, 652, 720, 1380, 1501, 2000, 2010
BstMCI CGRYCG 2 cut(s) 706, 1383
BstMWI GCNNNNNNNGC 5 cut(s) 32, 80, 101, 802, 1040
BstNI CCWGG 2 cut(s) 918, 1973
BstNSI RCATGY 1 cut(s) 384
BstPI GGTNACC 1 cut(s) 866
BstSCI CCNGG 2 cut(s) 916, 1971
BstSFI CTRYAG 1 cut(s) 1293
BstV1I GCAGC 6 cut(s) 10, 328, 1177, 1276, 1279, 2005
BstV2I GAAGAC 1 cut(s) 984
BstX2I RGATCY 1 cut(s) 77
BstYI RGATCY 1 cut(s) 77
Bsu15I ATCGAT 1 cut(s) 655
BsuI GTATCC 1 cut(s) 606
BsuRI GGCC 2 cut(s) 1113, 1582
BsuTUI ATCGAT 1 cut(s) 655
BtgI CCRYGG 1 cut(s) 880
BtgZI GCGATG 1 cut(s) 138
BtsCI GGATG 6 cut(s) 209, 344, 561, 580, 1234, 2032
BtsIMutI CAGTG 2 cut(s) 817, 837
BveI ACCTGC 1 cut(s) 409
Cac8I GCNNGC 3 cut(s) 170, 542, 1111
CciI TCATGA 1 cut(s) 1303
Cfr10I RCCGGY 1 cut(s) 1193
Cfr13I GGNCC 4 cut(s) 421, 499, 1120, 1588
ClaI ATCGAT 1 cut(s) 655
CseI GACGC 2 cut(s) 1196, 1481
Csp6I GTAC 7 cut(s) 374, 384, 476, 556, 1265, 1866, 1942
CviQI GTAC 7 cut(s) 374, 384, 476, 556, 1265, 1866, 1942
DdeI CTNAG 7 cut(s) 158, 204, 387, 663, 1222, 1512, 2055
DpnI GATC 9 cut(s) 79, 328, 432, 654, 722, 1382, 1503, 2002, 2012
DpnII GATC 9 cut(s) 77, 326, 430, 652, 720, 1380, 1501, 2000, 2010
EaeI YGGCCR 1 cut(s) 1580
Eam1104I CTCTTC 5 cut(s) 794, 880, 957, 1652, 1680
EarI CTCTTC 5 cut(s) 794, 880, 957, 1652, 1680
Eco130I CCWWGG 3 cut(s) 348, 880, 1123
Eco47I GGWCC 4 cut(s) 421, 499, 1120, 1588
Eco57I CTGAAG 3 cut(s) 263, 818, 1704
Eco91I GGTNACC 1 cut(s) 866
EcoNI CCTNNNNNAGG 1 cut(s) 1107
EcoO109I RGGNCCY 3 cut(s) 421, 1120, 1588
EcoO65I GGTNACC 1 cut(s) 866
EcoRII CCWGG 2 cut(s) 916, 1971
EcoT14I CCWWGG 3 cut(s) 348, 880, 1123
EcoT22I ATGCAT 3 cut(s) 58, 118, 1861
ErhI CCWWGG 3 cut(s) 348, 880, 1123
FalI AAGNNNNNCTT 2 cut(s) 1056, 1088
FaqI GGGAC 6 cut(s) 407, 650, 1084, 1579, 1601, 1775
FauI CCCGC 1 cut(s) 1149
Fnu4HI GCNGC 8 cut(s) 24, 229, 342, 780, 1191, 1290, 1293, 1994
FokI GGATG 6 cut(s) 196, 331, 548, 587, 1241, 2039
Fsp4HI GCNGC 8 cut(s) 24, 229, 342, 780, 1191, 1290, 1293, 1994
FspBI CTAG 6 cut(s) 18, 30, 92, 377, 863, 1286
GluI GCNGC 8 cut(s) 24, 229, 342, 780, 1191, 1290, 1293, 1994
GsaI CCCAGC 1 cut(s) 605
HaeIII GGCC 2 cut(s) 1113, 1582
HapII CCGG 4 cut(s) 482, 1194, 1274, 1898
HgaI GACGC 2 cut(s) 1196, 1481
HincII GTYRAC 1 cut(s) 1531
HindII GTYRAC 1 cut(s) 1531
HinfI GANTC 3 cut(s) 692, 1097, 1486
HpaII CCGG 4 cut(s) 482, 1194, 1274, 1898
HphI GGTGA 2 cut(s) 126, 830
Hpy166II GTNNAC 4 cut(s) 1420, 1496, 1531, 1866
Hpy188I TCNGA 8 cut(s) 121, 321, 664, 679, 697, 1849, 2005, 2047
Hpy188III TCNNGA 9 cut(s) 460, 482, 622, 689, 1023, 1131, 1304, 1610, 1682
Hpy8I GTNNAC 4 cut(s) 1420, 1496, 1531, 1866
Hpy99I CGWCG 1 cut(s) 2001
HpyCH4III ACNGT 7 cut(s) 555, 707, 818, 958, 1424, 1549, 1922
HpyF10VI GCNNNNNNNGC 5 cut(s) 32, 80, 101, 802, 1040
HpyF3I CTNAG 7 cut(s) 158, 204, 387, 663, 1222, 1512, 2055
Kpn2I TCCGGA 1 cut(s) 481
KpnI GGTACC 1 cut(s) 479
Kzo9I GATC 9 cut(s) 77, 326, 430, 652, 720, 1380, 1501, 2000, 2010
LmnI GCTCC 2 cut(s) 10, 1151
Lsp1109I GCAGC 6 cut(s) 10, 328, 1177, 1276, 1279, 2005
LweI GCATC 6 cut(s) 104, 125, 218, 353, 1546, 1827
MaeI CTAG 6 cut(s) 18, 30, 92, 377, 863, 1286
MaeIII GTNAC 9 cut(s) 64, 256, 646, 812, 866, 1020, 1330, 1402, 1543
MalI GATC 9 cut(s) 79, 328, 432, 654, 722, 1382, 1503, 2002, 2012
MboI GATC 9 cut(s) 77, 326, 430, 652, 720, 1380, 1501, 2000, 2010
MflI RGATCY 1 cut(s) 77
MhlI GDGCHC 1 cut(s) 1036
MlyI GAGTC 1 cut(s) 1106
MmeI TCCRAC 1 cut(s) 1041
Mph1103I ATGCAT 3 cut(s) 58, 118, 1861
MroI TCCGGA 1 cut(s) 481
MseI TTAA 8 cut(s) 233, 315, 534, 930, 972, 992, 1347, 1842
MslI CAYNNNNRTG 4 cut(s) 397, 520, 573, 1823
MspA1I CMGCKG 1 cut(s) 605
MspI CCGG 4 cut(s) 482, 1194, 1274, 1898
MspR9I CCNGG 2 cut(s) 918, 1973
Mva1269I GAATGC 1 cut(s) 1677
MvaI CCWGG 2 cut(s) 918, 1973
MwoI GCNNNNNNNGC 5 cut(s) 32, 80, 101, 802, 1040
NcoI CCATGG 1 cut(s) 880
NdeII GATC 9 cut(s) 77, 326, 430, 652, 720, 1380, 1501, 2000, 2010
NlaIV GGNNCC 7 cut(s) 6, 393, 423, 477, 1272, 1518, 1589
NmeAIII GCCGAG 1 cut(s) 1608
NmuCI GTSAC 3 cut(s) 64, 812, 1020
NsiI ATGCAT 3 cut(s) 58, 118, 1861
NspI RCATGY 1 cut(s) 384
OliI CACNNNNGTG 1 cut(s) 520
PagI TCATGA 1 cut(s) 1303
PctI GAATGC 1 cut(s) 1677
PfeI GAWTC 2 cut(s) 692, 1486
PkrI GCNGC 8 cut(s) 25, 230, 343, 781, 1192, 1291, 1294, 1995
Ple19I CGATCG 1 cut(s) 1383
PleI GAGTC 1 cut(s) 1105
PpsI GAGTC 1 cut(s) 1105
PpuMI RGGWCCY 3 cut(s) 421, 1120, 1588
PshBI ATTAAT 1 cut(s) 992
Psp5II RGGWCCY 3 cut(s) 421, 1120, 1588
Psp6I CCWGG 2 cut(s) 916, 1971
PspEI GGTNACC 1 cut(s) 866
PspFI CCCAGC 1 cut(s) 601
PspGI CCWGG 2 cut(s) 916, 1971
PspN4I GGNNCC 7 cut(s) 6, 393, 423, 477, 1272, 1518, 1589
PspPI GGNCC 4 cut(s) 421, 499, 1120, 1588
PspPPI RGGWCCY 3 cut(s) 421, 1120, 1588
PstI CTGCAG 1 cut(s) 1297
PsuI RGATCY 1 cut(s) 77
PvuI CGATCG 1 cut(s) 1383
PvuII CAGCTG 1 cut(s) 605
RsaI GTAC 7 cut(s) 375, 385, 477, 557, 1266, 1867, 1943
RsaNI GTAC 7 cut(s) 374, 384, 476, 556, 1265, 1866, 1942
RseI CAYNNNNRTG 4 cut(s) 397, 520, 573, 1823
SaqAI TTAA 8 cut(s) 233, 315, 534, 930, 972, 992, 1347, 1842
SatI GCNGC 8 cut(s) 24, 229, 342, 780, 1191, 1290, 1293, 1994
Sau3AI GATC 9 cut(s) 77, 326, 430, 652, 720, 1380, 1501, 2000, 2010
Sau96I GGNCC 4 cut(s) 421, 499, 1120, 1588
SchI GAGTC 1 cut(s) 1106
ScrFI CCNGG 2 cut(s) 918, 1973
SduI GDGCHC 1 cut(s) 1036
SfaNI GCATC 6 cut(s) 104, 125, 218, 353, 1546, 1827
SfcI CTRYAG 1 cut(s) 1293
SinI GGWCC 4 cut(s) 421, 499, 1120, 1588
SmiMI CAYNNNNRTG 4 cut(s) 397, 520, 573, 1823
SmlI CTYRAG 1 cut(s) 1506
SmoI CTYRAG 1 cut(s) 1506
SsiI CCGC 5 cut(s) 228, 780, 1156, 1213, 1939
SspI AATATT 3 cut(s) 46, 1390, 1696
SspMI CTAG 6 cut(s) 18, 30, 92, 377, 863, 1286
StyD4I CCNGG 2 cut(s) 916, 1971
StyI CCWWGG 3 cut(s) 348, 880, 1123
TaaI ACNGT 7 cut(s) 555, 707, 818, 958, 1424, 1549, 1922
TaqI TCGA 9 cut(s) 459, 621, 655, 702, 1356, 1379, 1383, 1484, 1821
TatI WGTACW 4 cut(s) 383, 555, 1264, 1865
TauI GCSGC 2 cut(s) 231, 782
TfiI GAWTC 2 cut(s) 692, 1486
Tru1I TTAA 8 cut(s) 233, 315, 534, 930, 972, 992, 1347, 1842
Tru9I TTAA 8 cut(s) 233, 315, 534, 930, 972, 992, 1347, 1842
TscAI CASTG 2 cut(s) 817, 844
TseFI GTSAC 3 cut(s) 64, 812, 1020
TseI GCWGC 6 cut(s) 23, 341, 1190, 1289, 1292, 1993
Tsp45I GTSAC 3 cut(s) 64, 812, 1020
TspRI CASTG 2 cut(s) 817, 844
VpaK11BI GGWCC 4 cut(s) 421, 499, 1120, 1588
VspI ATTAAT 1 cut(s) 992
XagI CCTNNNNNAGG 1 cut(s) 1107
XapI RAATTY 3 cut(s) 285, 530, 668
XceI RCATGY 1 cut(s) 384
XcmI CCANNNNNNNNNTGG 3 cut(s) 1121, 1414, 1445
XspI CTAG 6 cut(s) 18, 30, 92, 377, 863, 1286
Zsp2I ATGCAT 3 cut(s) 58, 118, 1861
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.