Rorug04G0291600

source UniProtKB

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000004
OG0000695
Physical Location & Seq
Reverse (-)
46555854 .. 46558360
2507 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug04G0291600.1

Sequence Viewer

Length: 879 bp
ATGGCCTCTGTAGCTTTCTTCACTCCACCGGCTCTGAAACTGGTCACCGGAAATGGGTCTTCTCGGAACCAGAGTTCTTGCTCGGAATTCTCAAATGGGTACCCTTGGTTTTCTTCTTCTTGCAGACCATTTAGAGCTCAGAAGTTCTCAGTTGGGCCCAGAAGGTCGGTGATTGTTGGGGCGAAGAAGAAGAACAACAACAAGGAGAGGAAGAAGGAAGATAAGCATAGCTTTGTGTCTAAACCTGATGAGGCTACTGGGCCTTTTCCTGAAGCTGTGCTGCTTAAACAGTCGAAAGTTCAGGAAGATGGGGCACTTCTCCCTGAGTTTGCGGACGCAGATGAAGAGAAGGTCTATGAATTTCTGAAGCTTCAGCTGCAAGATGATCTGGATGAGGAAAGACTGCGCCACTATGAAGTGGTTTATCTGATTCATGAGAAACATGCTGAAGAGGTGGGGAGTGTGAAGGAGAAAATTGAAGCTTTTCTAGGGGAGAAGAGAGCCAGGATATGGAGACTGAGTGATTGGGGAATGCGAAGACTAGCATACAAAATACAGAAAGCCAAGAACGCCTACTACGTTCTGATGAACTTTGAGATGGACGCCAAATGGATAAATGATTTCAAGAGCCTGTTGGACAAGGATGAAAGAGTCATTCGGCATCTGGTGATGAAGAGGGATGAGGCTATCACAGAGGACTGCCCTGCTCCTCCTGAGTTTCAGTCCATGAATGCAGGTACAGATTCTGAAGAGGATGACGAGGATGAGGATGATATGGAATACGATGATGAAGATTACGATGATGAAGATGGGATCATCATTGTTGACGCTGACAATGATGATGTAGAAGATAGTAAACTACTAGAACAACTAGTGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Peptidase_C48 Transposase_24

Protein Analysis

292

Amino Acids

33.68

Weight (kDa)

4.68

Isoelectric Point (pI)

50.57

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ribosomal_S6 PF01250 137 - 225 2.9e-24 Ribosomal protein S6
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 725
Acc65I GGTACC 1 cut(s) 99
AccB1I GGYRCC 1 cut(s) 99
AccB7I CCANNNNNTGG 1 cut(s) 510
AciI CCGC 1 cut(s) 332
AclWI GGATC 1 cut(s) 821
AcsI RAATTY 2 cut(s) 86, 359
AcuI CTGAAG 5 cut(s) 291, 356, 386, 468, 768
AcyI GRCGYC 1 cut(s) 603
AfaI GTAC 2 cut(s) 101, 739
AfiI CCNNNNNNNGG 2 cut(s) 54, 510
AgsI TTSAA 2 cut(s) 479, 625
AhlI ACTAGT 1 cut(s) 871
AjnI CCWGG 1 cut(s) 503
AloI GAACNNNNNNTCC 2 cut(s) 58, 90
AluBI AGCT 7 cut(s) 14, 137, 231, 275, 370, 376, 482
AluI AGCT 7 cut(s) 14, 137, 231, 275, 370, 376, 482
Alw21I GWGCWC 1 cut(s) 139
Alw26I GTCTC 1 cut(s) 508
AlwI GGATC 1 cut(s) 821
AlwNI CAGNNNCTG 1 cut(s) 746
AoxI GGCC 3 cut(s) 3, 155, 260
ApaI GGGCCC 1 cut(s) 159
ApeKI GCWGC 2 cut(s) 280, 376
ApoI RAATTY 2 cut(s) 86, 359
Asp700I GAANNNNTTC 1 cut(s) 483
Asp718I GGTACC 1 cut(s) 99
AspLEI GCGC 1 cut(s) 408
AspS9I GGNCC 3 cut(s) 155, 156, 260
AsuHPI GGTGA 3 cut(s) 37, 181, 679
BaeGI GKGCMC 2 cut(s) 159, 316
BanI GGYRCC 1 cut(s) 99
BanII GRGCYC 2 cut(s) 139, 159
BbsI GAAGAC 2 cut(s) 51, 544
Bbv12I GWGCWC 1 cut(s) 139
BbvI GCAGC 2 cut(s) 267, 363
BccI CCATC 3 cut(s) 302, 592, 803
BciT130I CCWGG 1 cut(s) 505
BcoDI GTCTC 1 cut(s) 508
BcuI ACTAGT 1 cut(s) 871
BfaI CTAG 4 cut(s) 488, 542, 863, 872
BfmI CTRYAG 1 cut(s) 9
BfuAI ACCTGC 1 cut(s) 725
BisI GCNGC 2 cut(s) 281, 377
BlsI GCNGC 2 cut(s) 282, 378
Bme1390I CCNGG 1 cut(s) 505
BmgT120I GGNCC 3 cut(s) 155, 156, 260
BmiI GGNNCC 3 cut(s) 68, 101, 157
BmrFI CCNGG 1 cut(s) 505
BmrI ACTGGG 1 cut(s) 267
BmsI GCATC 1 cut(s) 670
BmuI ACTGGG 1 cut(s) 267
BpiI GAAGAC 2 cut(s) 51, 544
BsaHI GRCGYC 1 cut(s) 603
BsaJI CCNNGG 1 cut(s) 104
BsaWI WCCGGW 1 cut(s) 47
BsaXI ACNNNNNCTCC 2 cut(s) 505, 535
Bsc4I CCNNNNNNNGG 2 cut(s) 54, 510
Bse118I RCCGGY 1 cut(s) 28
Bse1I ACTGG 2 cut(s) 45, 262
BseBI CCWGG 1 cut(s) 505
BseDI CCNNGG 1 cut(s) 104
BseGI GGATG 6 cut(s) 397, 649, 685, 760, 769, 775
BseLI CCNNNNNNNGG 2 cut(s) 54, 510
BseMII CTCAG 5 cut(s) 152, 162, 315, 509, 705
BseNI ACTGG 2 cut(s) 45, 262
BseRI GAGGAG 1 cut(s) 699
BseSI GKGCMC 2 cut(s) 159, 316
BseXI GCAGC 2 cut(s) 267, 363
BshFI GGCC 3 cut(s) 5, 157, 262
BshNI GGYRCC 1 cut(s) 99
BsiHKAI GWGCWC 1 cut(s) 139
BsiSI CCGG 2 cut(s) 29, 48
BslI CCNNNNNNNGG 2 cut(s) 54, 510
BsmAI GTCTC 1 cut(s) 508
BsmI GAATGC 2 cut(s) 537, 736
BsnI GGCC 3 cut(s) 5, 157, 262
Bsp120I GGGCCC 1 cut(s) 155
Bsp1286I GDGCHC 3 cut(s) 139, 159, 316
Bsp143I GATC 2 cut(s) 385, 813
BspACI CCGC 1 cut(s) 332
BspANI GGCC 3 cut(s) 5, 157, 262
BspCNI CTCAG 5 cut(s) 151, 161, 316, 510, 706
BspHI TCATGA 1 cut(s) 433
BspLI GGNNCC 3 cut(s) 68, 101, 157
BspMI ACCTGC 1 cut(s) 725
BspPI GGATC 1 cut(s) 821
BspT107I GGYRCC 1 cut(s) 99
BsrFI RCCGGY 1 cut(s) 28
BsrI ACTGG 2 cut(s) 45, 262
BssAI RCCGGY 1 cut(s) 28
BssECI CCNNGG 1 cut(s) 104
BssMI GATC 2 cut(s) 385, 813
BssNI GRCGYC 1 cut(s) 603
BssT1I CCWWGG 1 cut(s) 104
Bst2UI CCWGG 1 cut(s) 505
Bst4CI ACNGT 1 cut(s) 291
Bst6I CTCTTC 5 cut(s) 339, 444, 491, 668, 744
BstACI GRCGYC 1 cut(s) 603
BstDEI CTNAG 5 cut(s) 138, 148, 324, 518, 714
BstEII GGTNACC 1 cut(s) 43
BstF5I GGATG 6 cut(s) 397, 649, 685, 760, 769, 775
BstHHI GCGC 1 cut(s) 408
BstKTI GATC 2 cut(s) 388, 816
BstMAI GTCTC 1 cut(s) 508
BstMBI GATC 2 cut(s) 385, 813
BstMWI GCNNNNNNNGC 3 cut(s) 11, 376, 569
BstNI CCWGG 1 cut(s) 505
BstNSI RCATGY 1 cut(s) 446
BstPI GGTNACC 1 cut(s) 43
BstSCI CCNGG 1 cut(s) 503
BstSFI CTRYAG 1 cut(s) 9
BstSLI GKGCMC 2 cut(s) 159, 316
BstV1I GCAGC 2 cut(s) 267, 363
BstV2I GAAGAC 2 cut(s) 51, 544
BsuRI GGCC 3 cut(s) 5, 157, 262
BtsCI GGATG 6 cut(s) 397, 649, 685, 760, 769, 775
BveI ACCTGC 1 cut(s) 725
CaiI CAGNNNCTG 1 cut(s) 746
CciI TCATGA 1 cut(s) 433
CfoI GCGC 1 cut(s) 408
Cfr10I RCCGGY 1 cut(s) 28
Cfr13I GGNCC 3 cut(s) 155, 156, 260
CseI GACGC 3 cut(s) 344, 611, 836
Csp6I GTAC 2 cut(s) 100, 738
CviAII CATG 3 cut(s) 434, 443, 727
CviQI GTAC 2 cut(s) 100, 738
DdeI CTNAG 5 cut(s) 138, 148, 324, 518, 714
DpnI GATC 2 cut(s) 387, 815
DpnII GATC 2 cut(s) 385, 813
Eam1104I CTCTTC 5 cut(s) 339, 444, 491, 668, 744
EarI CTCTTC 5 cut(s) 339, 444, 491, 668, 744
Ecl136II GAGCTC 1 cut(s) 137
Eco130I CCWWGG 1 cut(s) 104
Eco24I GRGCYC 2 cut(s) 139, 159
Eco53kI GAGCTC 1 cut(s) 137
Eco57I CTGAAG 5 cut(s) 291, 356, 386, 468, 768
Eco91I GGTNACC 1 cut(s) 43
EcoICRI GAGCTC 1 cut(s) 137
EcoO65I GGTNACC 1 cut(s) 43
EcoRI GAATTC 1 cut(s) 86
EcoRII CCWGG 1 cut(s) 503
EcoT14I CCWWGG 1 cut(s) 104
EcoT38I GRGCYC 2 cut(s) 139, 159
ErhI CCWWGG 1 cut(s) 104
FaeI CATG 3 cut(s) 437, 446, 730
FaiI YATR 9 cut(s) 228, 357, 414, 435, 444, 511, 547, 728, 776
FalI AAGNNNNNCTT 2 cut(s) 215, 247
FatI CATG 3 cut(s) 433, 442, 726
Fnu4HI GCNGC 2 cut(s) 281, 377
FokI GGATG 6 cut(s) 404, 656, 692, 767, 776, 782
FriOI GRGCYC 2 cut(s) 139, 159
Fsp4HI GCNGC 2 cut(s) 281, 377
FspBI CTAG 4 cut(s) 488, 542, 863, 872
GlaI GCGC 1 cut(s) 407
GluI GCNGC 2 cut(s) 281, 377
HaeIII GGCC 3 cut(s) 5, 157, 262
HapII CCGG 2 cut(s) 29, 48
HgaI GACGC 3 cut(s) 344, 611, 836
HhaI GCGC 1 cut(s) 408
Hin1I GRCGYC 1 cut(s) 603
Hin1II CATG 3 cut(s) 437, 446, 730
Hin6I GCGC 1 cut(s) 406
HinP1I GCGC 1 cut(s) 406
HincII GTYRAC 1 cut(s) 826
HindII GTYRAC 1 cut(s) 826
HindIII AAGCTT 2 cut(s) 368, 480
HinfI GANTC 3 cut(s) 430, 651, 743
HpaII CCGG 2 cut(s) 29, 48
HphI GGTGA 3 cut(s) 37, 181, 679
Hpy166II GTNNAC 2 cut(s) 826, 857
Hpy188I TCNGA 8 cut(s) 36, 66, 85, 141, 366, 429, 585, 748
Hpy188III TCNNGA 6 cut(s) 269, 302, 389, 434, 625, 713
Hpy8I GTNNAC 2 cut(s) 826, 857
HpyAV CCTTC 4 cut(s) 156, 208, 343, 460
HpyCH4III ACNGT 1 cut(s) 291
HpyCH4IV ACGT 1 cut(s) 579
HpyCH4V TGCA 3 cut(s) 123, 379, 734
HpyF10VI GCNNNNNNNGC 3 cut(s) 11, 376, 569
HpyF3I CTNAG 5 cut(s) 138, 148, 324, 518, 714
HpySE526I ACGT 1 cut(s) 579
Hsp92I GRCGYC 1 cut(s) 603
Hsp92II CATG 3 cut(s) 437, 446, 730
HspAI GCGC 1 cut(s) 406
KpnI GGTACC 1 cut(s) 103
Kzo9I GATC 2 cut(s) 385, 813
LmnI GCTCC 1 cut(s) 712
Lsp1109I GCAGC 2 cut(s) 267, 363
LweI GCATC 1 cut(s) 670
MaeI CTAG 4 cut(s) 488, 542, 863, 872
MaeII ACGT 1 cut(s) 579
MaeIII GTNAC 1 cut(s) 43
MalI GATC 2 cut(s) 387, 815
MboI GATC 2 cut(s) 385, 813
MhlI GDGCHC 3 cut(s) 139, 159, 316
MluCI AATT 3 cut(s) 86, 359, 474
MlyI GAGTC 1 cut(s) 660
MmeI TCCRAC 1 cut(s) 615
MroXI GAANNNNTTC 1 cut(s) 483
MseI TTAA 1 cut(s) 285
MspA1I CMGCKG 1 cut(s) 376
MspI CCGG 2 cut(s) 29, 48
MspR9I CCNGG 1 cut(s) 505
Mva1269I GAATGC 2 cut(s) 537, 736
MvaI CCWGG 1 cut(s) 505
MwoI GCNNNNNNNGC 3 cut(s) 11, 376, 569
NdeII GATC 2 cut(s) 385, 813
NlaIII CATG 3 cut(s) 437, 446, 730
NlaIV GGNNCC 3 cut(s) 68, 101, 157
NmuCI GTSAC 1 cut(s) 43
NspI RCATGY 1 cut(s) 446
PagI TCATGA 1 cut(s) 433
PcsI WCGNNNNNNNCGW 1 cut(s) 576
PctI GAATGC 2 cut(s) 537, 736
PdmI GAANNNNTTC 1 cut(s) 483
PfeI GAWTC 2 cut(s) 430, 743
PflMI CCANNNNNTGG 1 cut(s) 510
PkrI GCNGC 2 cut(s) 282, 378
PleI GAGTC 1 cut(s) 659
PpsI GAGTC 1 cut(s) 659
Psp124BI GAGCTC 1 cut(s) 139
Psp6I CCWGG 1 cut(s) 503
PspEI GGTNACC 1 cut(s) 43
PspGI CCWGG 1 cut(s) 503
PspN4I GGNNCC 3 cut(s) 68, 101, 157
PspOMI GGGCCC 1 cut(s) 155
PspPI GGNCC 3 cut(s) 155, 156, 260
PsrI GAACNNNNNNTAC 2 cut(s) 560, 592
PstNI CAGNNNCTG 1 cut(s) 746
PvuII CAGCTG 1 cut(s) 376
RsaI GTAC 2 cut(s) 101, 739
RsaNI GTAC 2 cut(s) 100, 738
SacI GAGCTC 1 cut(s) 139
SaqAI TTAA 1 cut(s) 285
SatI GCNGC 2 cut(s) 281, 377
Sau3AI GATC 2 cut(s) 385, 813
Sau96I GGNCC 3 cut(s) 155, 156, 260
SchI GAGTC 1 cut(s) 660
ScrFI CCNGG 1 cut(s) 505
SduI GDGCHC 3 cut(s) 139, 159, 316
SfaNI GCATC 1 cut(s) 670
SfcI CTRYAG 1 cut(s) 9
SpeI ACTAGT 1 cut(s) 871
Sse9I AATT 3 cut(s) 86, 359, 474
SsiI CCGC 1 cut(s) 332
SspMI CTAG 4 cut(s) 488, 542, 863, 872
SstI GAGCTC 1 cut(s) 139
StyD4I CCNGG 1 cut(s) 503
StyI CCWWGG 1 cut(s) 104
TaaI ACNGT 1 cut(s) 291
TaiI ACGT 1 cut(s) 582
TaqI TCGA 1 cut(s) 293
TasI AATT 3 cut(s) 86, 359, 474
TfiI GAWTC 2 cut(s) 430, 743
Tru1I TTAA 1 cut(s) 285
Tru9I TTAA 1 cut(s) 285
TseFI GTSAC 1 cut(s) 43
TseI GCWGC 2 cut(s) 280, 376
Tsp45I GTSAC 1 cut(s) 43
Van91I CCANNNNNTGG 1 cut(s) 510
XapI RAATTY 2 cut(s) 86, 359
XceI RCATGY 1 cut(s) 446
XmnI GAANNNNTTC 1 cut(s) 483
XspI CTAG 4 cut(s) 488, 542, 863, 872
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.