Rorug06G0405200

Cytochrome p450

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000006
OG0000192
Physical Location & Seq
Forward (+)
54893587 .. 54897219
3633 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug06G0405200.1

Sequence Viewer

Length: 993 bp
ATGATGTACCTGAGCTCAAACTCAAACCCTATAATGAGAACTACTTCTTCACCCTTGCCTGATCTCTCTTTACAGATCAGCCCACCATCAGATCACTACGAGATGGGTTTAGCAAGAAAAGCACTTAACGGTGATAGGAGCTCAACCACCGACTCCACTAGTAGTGGGAGTGATTTGAGTCATGAAAATGTTCTCTACCACTTGGATCAGAGGACTAATTATAATCTCACTTCCGCTGCGCCCGAGCCAAATTTGAGTTTAGGTCTTGAAATGAAGGATATCATGAGTAGTAGTACTAGTTCAACACCTCTACTGCCAAGAAACTTCAATCATCATCATCCTCAAATCTACGGCCGAGATTTCAAAAGAAGCGCCAGAACTGTCGGGAATGTGAAAAGAAGCGTCAGAGCTCCGAGGATGCGGTGGACTACAACCCTTCATGCCCATTTCGTTCATGCAGTCCAGCTTCTCGGCGGCCATGAAAGAGCAACGCCAAAATCGGTGTTAGAGTTGATGAATGTGAAGGATCTAACTCTTGCACACGTGAAGAGTCATTTGCAGATGTATAGAACAGTGAAGAGCACTGACCACAAAGGAACAGGAAACGAAGGGCAGCAGATAGATATGGGTTTGGACCAAAATCCAGGGAATAATAATGCTCAAGTAAATGGATCAGTACTACCTTGTGATTCTGAGAAAGCTATTAATTCACCTCCAACACTGCTTCTGCAAAATCCCAGCAGAGGATCATGGCATTTTGCAAGGGAGACAAATGGCAGCTGCAAACTAAGTCATGGAAATTCTTTGACGTATTCTCATTCCCCATCCTCAAATGGCACCAAGGTGGAGGAGGAGGATTCGGGTTCAGGCCGCCAGTTGGATTGTAGCTCCTTGTCATCTTCGGATCATATGTATCTTAACCTAGAGTTCACCCTTGGAAGGCCAAGTGGCGAATGGTCTATGCTCAACACCCTCAACCTTCAAATGACTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
p450

Protein Analysis

330

Amino Acids

36.35

Weight (kDa)

7.32

Isoelectric Point (pI)

44.15

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Myb_DNA-binding PF00249 139 - 190 1.2e-06 Myb-like DNA-binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000192)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G36110 AT5G36110 AT5G36130 AT5G36140
fragaria_vesca FvH4_2g39160 FvH4_3g15030 FvH4_3g38021 FvH4_3g38080 FvH4_4g00250 FvH4_4g01400 FvH4_5g18260 FvH4_5g18260 FvH4_5g29490 FvH4_7g03260 FvH4_7g12550 FvH4_7g12560 FvH4_7g14310 FvH4_7g14330
malus_domestica MD04G1002500.v1.1 MD11G1006100.v1.1 MD11G1157200.v1.1 MD11G1157300.v1.1 MD13G1116400.v1.1 MD13G1219600.v1.1 MD13G1284800.v1.1 MD16G1278300.v1.1 MD17G1219500.v1.1
prunus_persica Prupe.1G002500_v2.0.a1 Prupe.6G120400_v2.0.a1
pyrus_communis pycom04g00270 pycom10g20130 pycom11g12750 pycom16g24900 pycom17g22420 pycom420g00590
rosa_chinensis RchiOBHm_Chr1g0325391 RchiOBHm_Chr1g0340921 RchiOBHm_Chr1g0355301 RchiOBHm_Chr1g0355311 RchiOBHm_Chr1g0355331 RchiOBHm_Chr1g0355341 RchiOBHm_Chr2g0153901 RchiOBHm_Chr4g0384961 RchiOBHm_Chr4g0384991 RchiOBHm_Chr4g0393781 RchiOBHm_Chr4g0433801 RchiOBHm_Chr5g0024681 RchiOBHm_Chr5g0024711 RchiOBHm_Chr5g0024721 RchiOBHm_Chr5g0024791 RchiOBHm_Chr7g0177321 RchiOBHm_Chr7g0177351 RchiOBHm_Chr7g0177361
rosa_laevigata RLG00000001838 RLG00000005543 RLG00000010261 RLG00000028154 RLG00000028157 RLG00000028158 RLG00000028162 RLG00000030142 RLG00000032812 RLG00000032813 RLG00000032814
rosa_multiflora Rmu_co8235405.1_g000001 Rmu_co8395679.1_g000001 Rmu_co8439995.1_g000001 Rmu_sc0000999.1_g000011 Rmu_sc0001171.1_g000026 Rmu_sc0002766.1_g000009 Rmu_sc0003822.1_g000014 Rmu_sc0004135.1_g000001 Rmu_sc0004135.1_g000004 Rmu_sc0004135.1_g000006 Rmu_sc0004135.1_g000012 Rmu_sc0004135.1_g000017 Rmu_sc0004135.1_g000020 Rmu_sc0004637.1_g000001 Rmu_sc0005310.1_g000005 Rmu_sc0006151.1_g000002 Rmu_sc0007192.1_g000013 Rmu_sc0008633.1_g000002 Rmu_sc0020285.1_g000001
rosa_roxburghii Rroxscaffold_1G00054590 Rroxscaffold_2G00098180 Rroxscaffold_3G00234050 Rroxscaffold_3G00236820 Rroxscaffold_3G00275940 Rroxscaffold_4G00300500 Rroxscaffold_4G00300510 Rroxscaffold_5G00332950 Rroxscaffold_7G00188110
rosa_rugosa Rorug01G0051800 Rorug01G0051900 Rorug01G0052200 Rorug01G0052300 Rorug01G0052300 Rorug01G0246500 Rorug01G0246500 Rorug01G0246600 Rorug02G0411000 Rorug03G0298400 Rorug03G0298400 Rorug06G0404900 Rorug06G0405200 Rorug07G0209500 Rorug07G0227800
rosa_samantha Rh1AG066900 Rh1AG258800 Rh1AG259000 Rh1BG055200 Rh1BG055300 Rh1BG228400 Rh1BG228500 Rh1BG228600 Rh1BG228700 Rh1CG068300 Rh1CG242400 Rh1CG242600 Rh1CG242800 Rh1CG242900 Rh1CG243000 Rh1DG073000 Rh1DG256200 Rh1DG256300 Rh1DG256400 Rh1DG256500 Rh4AG002300 Rh4AG326300 Rh4BG001800 Rh4CG002200 Rh4DG001800 Rh5BG173900 Rh5CG190900 Rh5DG175500 Rh7AG004800 Rh7AG004900 Rh7AG349600 Rh7AG375800 Rh7BG004900 Rh7BG362800 Rh7CG004700 Rh7CG366800 Rh7CG394300 Rh7DG004600 Rh7DG004700 Rh7DG374000
rosa_wichuraiana Rw0G008010 Rw0G008840 Rw1G022840 Rw1G022850 Rw4G000710 Rw4G028240 Rw4G028290 Rw5G015960 Rw7G000400 Rw7G029670 Rw7G031540

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 222
AccB1I GGYRCC 1 cut(s) 836
AciI CCGC 4 cut(s) 234, 421, 474, 871
AclWI GGATC 5 cut(s) 213, 534, 679, 754, 912
AcoI YGGCCR 2 cut(s) 352, 475
AcsI RAATTY 2 cut(s) 250, 799
AcvI CACGTG 1 cut(s) 544
AfaI GTAC 3 cut(s) 8, 295, 678
AfiI CCNNNNNNNGG 3 cut(s) 743, 877, 939
AflIII ACRYGT 1 cut(s) 541
AgsI TTSAA 5 cut(s) 269, 303, 328, 364, 983
AhlI ACTAGT 2 cut(s) 158, 296
AjnI CCWGG 1 cut(s) 643
AleI CACNNNNGTG 1 cut(s) 842
AluBI AGCT 7 cut(s) 15, 141, 410, 466, 701, 780, 888
AluI AGCT 7 cut(s) 15, 141, 410, 466, 701, 780, 888
Alw21I GWGCWC 4 cut(s) 17, 143, 412, 584
Alw26I GTCTC 1 cut(s) 761
AlwI GGATC 5 cut(s) 213, 534, 679, 754, 912
Ama87I CYCGRG 1 cut(s) 242
AoxI GGCC 4 cut(s) 352, 475, 868, 941
ApeKI GCWGC 4 cut(s) 236, 613, 777, 780
ApoI RAATTY 2 cut(s) 250, 799
AseI ATTAAT 1 cut(s) 705
Asp700I GAANNNNTTC 2 cut(s) 43, 189
AspLEI GCGC 2 cut(s) 241, 374
AspS9I GGNCC 1 cut(s) 634
AsuHPI GGTGA 4 cut(s) 42, 143, 702, 922
AvaI CYCGRG 1 cut(s) 242
AvaII GGWCC 1 cut(s) 634
BanI GGYRCC 1 cut(s) 836
BanII GRGCYC 3 cut(s) 17, 143, 412
BbrPI CACGTG 1 cut(s) 544
Bbv12I GWGCWC 4 cut(s) 17, 143, 412, 584
BbvI GCAGC 4 cut(s) 223, 625, 767, 789
BccI CCATC 3 cut(s) 94, 97, 832
BceAI ACGGC 1 cut(s) 367
BciT130I CCWGG 1 cut(s) 645
BcoDI GTCTC 1 cut(s) 761
BcuI ACTAGT 2 cut(s) 158, 296
BfaI CTAG 3 cut(s) 159, 297, 923
BfoI RGCGCY 1 cut(s) 375
BisI GCNGC 6 cut(s) 237, 475, 614, 778, 781, 871
BlsI GCNGC 6 cut(s) 238, 476, 615, 779, 782, 872
BmcAI AGTACT 2 cut(s) 295, 678
Bme1390I CCNGG 1 cut(s) 645
Bme18I GGWCC 1 cut(s) 634
BmeT110I CYCGRG 1 cut(s) 242
BmgT120I GGNCC 1 cut(s) 634
BmiI GGNNCC 1 cut(s) 838
BmrFI CCNGG 1 cut(s) 645
BmsI GCATC 1 cut(s) 408
Bpu10I CCTNAGC 1 cut(s) 11
BpuEI CTTGAG 1 cut(s) 645
BsaAI YACGTR 1 cut(s) 544
BsaJI CCNNGG 4 cut(s) 413, 644, 840, 934
Bsc4I CCNNNNNNNGG 3 cut(s) 743, 877, 939
Bse1I ACTGG 1 cut(s) 874
BseBI CCWGG 1 cut(s) 645
BseDI CCNNGG 4 cut(s) 413, 644, 840, 934
BseGI GGATG 3 cut(s) 337, 423, 824
BseLI CCNNNNNNNGG 3 cut(s) 743, 877, 939
BseMII CTCAG 1 cut(s) 684
BseNI ACTGG 1 cut(s) 874
BseRI GAGGAG 2 cut(s) 863, 866
BseX3I CGGCCG 1 cut(s) 352
BseXI GCAGC 4 cut(s) 223, 625, 767, 789
BseYI CCCAGC 1 cut(s) 737
Bsh1285I CGRYCG 1 cut(s) 355
BshFI GGCC 4 cut(s) 354, 477, 870, 943
BshNI GGYRCC 1 cut(s) 836
BsiEI CGRYCG 1 cut(s) 355
BsiHKAI GWGCWC 4 cut(s) 17, 143, 412, 584
BsiHKCI CYCGRG 1 cut(s) 242
BslI CCNNNNNNNGG 3 cut(s) 743, 877, 939
BsmAI GTCTC 1 cut(s) 761
BsnI GGCC 4 cut(s) 354, 477, 870, 943
BsoBI CYCGRG 1 cut(s) 242
Bsp1286I GDGCHC 4 cut(s) 17, 143, 412, 584
Bsp143I GATC 8 cut(s) 61, 75, 91, 205, 526, 671, 746, 904
BspACI CCGC 4 cut(s) 234, 421, 474, 871
BspANI GGCC 4 cut(s) 354, 477, 870, 943
BspCNI CTCAG 1 cut(s) 685
BspHI TCATGA 2 cut(s) 181, 282
BspLI GGNNCC 1 cut(s) 838
BspPI GGATC 5 cut(s) 213, 534, 679, 754, 912
BspQI GCTCTTC 1 cut(s) 572
BspT107I GGYRCC 1 cut(s) 836
BsrI ACTGG 1 cut(s) 874
BssECI CCNNGG 4 cut(s) 413, 644, 840, 934
BssMI GATC 8 cut(s) 61, 75, 91, 205, 526, 671, 746, 904
BssT1I CCWWGG 2 cut(s) 840, 934
Bst2UI CCWGG 1 cut(s) 645
Bst4CI ACNGT 3 cut(s) 131, 382, 574
Bst6I CTCTTC 2 cut(s) 542, 572
BstBAI YACGTR 1 cut(s) 544
BstDEI CTNAG 3 cut(s) 11, 693, 788
BstF5I GGATG 3 cut(s) 337, 423, 824
BstH2I RGCGCY 1 cut(s) 375
BstHHI GCGC 2 cut(s) 241, 374
BstKTI GATC 8 cut(s) 64, 78, 94, 208, 529, 674, 749, 907
BstMAI GTCTC 1 cut(s) 761
BstMBI GATC 8 cut(s) 61, 75, 91, 205, 526, 671, 746, 904
BstMCI CGRYCG 1 cut(s) 355
BstMWI GCNNNNNNNGC 1 cut(s) 119
BstNI CCWGG 1 cut(s) 645
BstSCI CCNGG 1 cut(s) 643
BstV1I GCAGC 4 cut(s) 223, 625, 767, 789
BstX2I RGATCY 1 cut(s) 526
BstYI RGATCY 1 cut(s) 526
BstZI CGGCCG 1 cut(s) 352
BsuRI GGCC 4 cut(s) 354, 477, 870, 943
BtsCI GGATG 3 cut(s) 337, 423, 824
BtsI GCAGTG 1 cut(s) 719
BtsIMutI CAGTG 3 cut(s) 579, 582, 719
CciI TCATGA 2 cut(s) 181, 282
CfoI GCGC 2 cut(s) 241, 374
Cfr13I GGNCC 1 cut(s) 634
CseI GACGC 1 cut(s) 391
Csp6I GTAC 3 cut(s) 7, 294, 677
CviAII CATG 7 cut(s) 182, 283, 440, 455, 479, 750, 794
CviQI GTAC 3 cut(s) 7, 294, 677
DdeI CTNAG 3 cut(s) 11, 693, 788
DpnI GATC 8 cut(s) 63, 77, 93, 207, 528, 673, 748, 906
DpnII GATC 8 cut(s) 61, 75, 91, 205, 526, 671, 746, 904
EaeI YGGCCR 2 cut(s) 352, 475
EagI CGGCCG 1 cut(s) 352
Eam1104I CTCTTC 2 cut(s) 542, 572
EarI CTCTTC 2 cut(s) 542, 572
Ecl136II GAGCTC 3 cut(s) 15, 141, 410
EclXI CGGCCG 1 cut(s) 352
Eco130I CCWWGG 2 cut(s) 840, 934
Eco24I GRGCYC 3 cut(s) 17, 143, 412
Eco32I GATATC 1 cut(s) 280
Eco47I GGWCC 1 cut(s) 634
Eco52I CGGCCG 1 cut(s) 352
Eco53kI GAGCTC 3 cut(s) 15, 141, 410
Eco72I CACGTG 1 cut(s) 544
Eco88I CYCGRG 1 cut(s) 242
EcoICRI GAGCTC 3 cut(s) 15, 141, 410
EcoRII CCWGG 1 cut(s) 643
EcoRV GATATC 1 cut(s) 280
EcoT14I CCWWGG 2 cut(s) 840, 934
EcoT38I GRGCYC 3 cut(s) 17, 143, 412
ErhI CCWWGG 2 cut(s) 840, 934
FaeI CATG 7 cut(s) 185, 286, 443, 458, 482, 753, 797
FatI CATG 7 cut(s) 181, 282, 439, 454, 478, 749, 793
FauNDI CATATG 1 cut(s) 909
Fnu4HI GCNGC 6 cut(s) 237, 475, 614, 778, 781, 871
FokI GGATG 3 cut(s) 324, 430, 811
FriOI GRGCYC 3 cut(s) 17, 143, 412
Fsp4HI GCNGC 6 cut(s) 237, 475, 614, 778, 781, 871
FspBI CTAG 3 cut(s) 159, 297, 923
GlaI GCGC 2 cut(s) 240, 373
GluI GCNGC 6 cut(s) 237, 475, 614, 778, 781, 871
GsaI CCCAGC 1 cut(s) 741
HaeII RGCGCY 1 cut(s) 375
HaeIII GGCC 4 cut(s) 354, 477, 870, 943
HgaI GACGC 1 cut(s) 391
HhaI GCGC 2 cut(s) 241, 374
Hin1II CATG 7 cut(s) 185, 286, 443, 458, 482, 753, 797
Hin6I GCGC 2 cut(s) 239, 372
HinP1I GCGC 2 cut(s) 239, 372
HinfI GANTC 5 cut(s) 152, 178, 550, 689, 857
HphI GGTGA 4 cut(s) 42, 143, 702, 922
Hpy166II GTNNAC 2 cut(s) 426, 930
Hpy188I TCNGA 6 cut(s) 91, 210, 407, 414, 694, 904
Hpy188III TCNNGA 4 cut(s) 182, 266, 283, 385
Hpy8I GTNNAC 2 cut(s) 426, 930
HpyAV CCTTC 6 cut(s) 268, 446, 517, 602, 933, 989
HpyCH4III ACNGT 3 cut(s) 131, 382, 574
HpyCH4IV ACGT 2 cut(s) 543, 809
HpyCH4V TGCA 6 cut(s) 458, 539, 559, 730, 761, 783
HpyF10VI GCNNNNNNNGC 1 cut(s) 119
HpyF3I CTNAG 3 cut(s) 11, 693, 788
HpySE526I ACGT 2 cut(s) 543, 809
Hsp92II CATG 7 cut(s) 185, 286, 443, 458, 482, 753, 797
HspAI GCGC 2 cut(s) 239, 372
Kzo9I GATC 8 cut(s) 61, 75, 91, 205, 526, 671, 746, 904
LguI GCTCTTC 1 cut(s) 572
LmnI GCTCC 3 cut(s) 138, 415, 893
Lsp1109I GCAGC 4 cut(s) 223, 625, 767, 789
LweI GCATC 1 cut(s) 408
MaeI CTAG 3 cut(s) 159, 297, 923
MaeII ACGT 2 cut(s) 543, 809
MalI GATC 8 cut(s) 63, 77, 93, 207, 528, 673, 748, 906
MboI GATC 8 cut(s) 61, 75, 91, 205, 526, 671, 746, 904
MboII GAAGA 4 cut(s) 39, 559, 589, 891
MflI RGATCY 1 cut(s) 526
MhlI GDGCHC 4 cut(s) 17, 143, 412, 584
MluCI AATT 4 cut(s) 217, 250, 706, 799
MlyI GAGTC 3 cut(s) 146, 187, 559
MmeI TCCRAC 2 cut(s) 740, 858
MroXI GAANNNNTTC 2 cut(s) 43, 189
MseI TTAA 3 cut(s) 126, 705, 918
MslI CAYNNNNRTG 2 cut(s) 186, 842
MspA1I CMGCKG 2 cut(s) 236, 780
MspR9I CCNGG 1 cut(s) 645
MvaI CCWGG 1 cut(s) 645
MwoI GCNNNNNNNGC 1 cut(s) 119
NdeI CATATG 1 cut(s) 909
NdeII GATC 8 cut(s) 61, 75, 91, 205, 526, 671, 746, 904
NlaIII CATG 7 cut(s) 185, 286, 443, 458, 482, 753, 797
NlaIV GGNNCC 1 cut(s) 838
NmeAIII GCCGAG 2 cut(s) 380, 450
OliI CACNNNNGTG 1 cut(s) 842
PagI TCATGA 2 cut(s) 181, 282
PciSI GCTCTTC 1 cut(s) 572
PdmI GAANNNNTTC 2 cut(s) 43, 189
PfeI GAWTC 2 cut(s) 689, 857
PkrI GCNGC 6 cut(s) 238, 476, 615, 779, 782, 872
PleI GAGTC 3 cut(s) 146, 186, 558
PmaCI CACGTG 1 cut(s) 544
PmlI CACGTG 1 cut(s) 544
PpsI GAGTC 3 cut(s) 146, 186, 558
Ppu21I YACGTR 1 cut(s) 544
PshBI ATTAAT 1 cut(s) 705
PsiI TTATAA 1 cut(s) 222
Psp124BI GAGCTC 3 cut(s) 17, 143, 412
Psp6I CCWGG 1 cut(s) 643
PspCI CACGTG 1 cut(s) 544
PspFI CCCAGC 1 cut(s) 737
PspGI CCWGG 1 cut(s) 643
PspN4I GGNNCC 1 cut(s) 838
PspPI GGNCC 1 cut(s) 634
PsrI GAACNNNNNNTAC 2 cut(s) 283, 315
PsuI RGATCY 1 cut(s) 526
PvuII CAGCTG 1 cut(s) 780
RsaI GTAC 3 cut(s) 8, 295, 678
RsaNI GTAC 3 cut(s) 7, 294, 677
RseI CAYNNNNRTG 2 cut(s) 186, 842
SacI GAGCTC 3 cut(s) 17, 143, 412
SapI GCTCTTC 1 cut(s) 572
SaqAI TTAA 3 cut(s) 126, 705, 918
SatI GCNGC 6 cut(s) 237, 475, 614, 778, 781, 871
Sau3AI GATC 8 cut(s) 61, 75, 91, 205, 526, 671, 746, 904
Sau96I GGNCC 1 cut(s) 634
ScaI AGTACT 2 cut(s) 295, 678
SchI GAGTC 3 cut(s) 146, 187, 559
ScrFI CCNGG 1 cut(s) 645
SduI GDGCHC 4 cut(s) 17, 143, 412, 584
SfaNI GCATC 1 cut(s) 408
SinI GGWCC 1 cut(s) 634
SmiMI CAYNNNNRTG 2 cut(s) 186, 842
SmlI CTYRAG 1 cut(s) 660
SmoI CTYRAG 1 cut(s) 660
SpeI ACTAGT 2 cut(s) 158, 296
Sse9I AATT 4 cut(s) 217, 250, 706, 799
SsiI CCGC 4 cut(s) 234, 421, 474, 871
SspMI CTAG 3 cut(s) 159, 297, 923
SstI GAGCTC 3 cut(s) 17, 143, 412
StyD4I CCNGG 1 cut(s) 643
StyI CCWWGG 2 cut(s) 840, 934
TaaI ACNGT 3 cut(s) 131, 382, 574
TaiI ACGT 2 cut(s) 546, 812
TasI AATT 4 cut(s) 217, 250, 706, 799
TatI WGTACW 2 cut(s) 293, 676
TauI GCSGC 2 cut(s) 477, 873
TfiI GAWTC 2 cut(s) 689, 857
Tru1I TTAA 3 cut(s) 126, 705, 918
Tru9I TTAA 3 cut(s) 126, 705, 918
TscAI CASTG 3 cut(s) 579, 589, 726
TseI GCWGC 4 cut(s) 236, 613, 777, 780
TspDTI ATGAA 6 cut(s) 198, 287, 428, 443, 495, 530
TspRI CASTG 3 cut(s) 579, 589, 726
VpaK11BI GGWCC 1 cut(s) 634
VspI ATTAAT 1 cut(s) 705
XapI RAATTY 2 cut(s) 250, 799
XcmI CCANNNNNNNNNTGG 1 cut(s) 951
XmnI GAANNNNTTC 2 cut(s) 43, 189
XspI CTAG 3 cut(s) 159, 297, 923
ZrmI AGTACT 2 cut(s) 295, 678
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.