Rw4G028290

Cytochrome p450

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr4
OG0000192
Physical Location & Seq
Reverse (-)
55001042 .. 55004647
3606 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw4G028290.1

Sequence Viewer

Length: 657 bp
ATGGCGTCTACAGGAAACAAAATGCATGATCTTTTGTCTTATATGATTTCTACGCCAGACCCGGTCACCGGAAGGTTCATGCCGGAGAATGAAATTGCTGATAAAATTATGGGGAATGTGGCCGCTTCGTTCAACCCTCCAGCTATGACTACTACTTTTGTCGTGAAGTTCTTGGGCGAAAGACCTGACATTTATGAAAAAGTCCGAAGTGAACAACTGGAGATTTTGAAGTCCAAAAAATCTGGCGAGGCACTCAATTGGGATGATATGCATAAAATGAAGTATTCGTGGAACGTAGCCCTGGAGGCGATGAGGCTCATACCACCTCTTCAGGGAACATTTAGAGAGGCTGCAACAGACTTCACTTACCAAGGTTATACAATTCCTAAAGGATGGAAGGTCTACTGGACAGTGAGCACCACAACCATGAACCCTGAATATTTTCCAGCGCCAGAAGAGTTTGATCCTACAAGCGGTCCTCGAATTTGCCCCGGAAAGGACTACGCTCGTCTGCAAATTCTTAGTTTCATGCACCATCTTGTTATAAGATTCAAATGGGAACTTGTGAACCCTAATTGCAAGATCACAGGTGGAATGAATCCCTTTCCAGTTGATGGGCTGAAGGTTCGCCTCCAAGCTCAACCCAGTCAAGCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
p450

Protein Analysis

218

Amino Acids

24.77

Weight (kDa)

7.74

Isoelectric Point (pI)

45.91

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
p450 PF00067 7 - 157 1.2e-25 Cytochrome P450
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000192)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G36110 AT5G36110 AT5G36130 AT5G36140
fragaria_vesca FvH4_2g39160 FvH4_3g15030 FvH4_3g38021 FvH4_3g38080 FvH4_4g00250 FvH4_4g01400 FvH4_5g18260 FvH4_5g18260 FvH4_5g29490 FvH4_7g03260 FvH4_7g12550 FvH4_7g12560 FvH4_7g14310 FvH4_7g14330
malus_domestica MD04G1002500.v1.1 MD11G1006100.v1.1 MD11G1157200.v1.1 MD11G1157300.v1.1 MD13G1116400.v1.1 MD13G1219600.v1.1 MD13G1284800.v1.1 MD16G1278300.v1.1 MD17G1219500.v1.1
prunus_persica Prupe.1G002500_v2.0.a1 Prupe.6G120400_v2.0.a1
pyrus_communis pycom04g00270 pycom10g20130 pycom11g12750 pycom16g24900 pycom17g22420 pycom420g00590
rosa_chinensis RchiOBHm_Chr1g0325391 RchiOBHm_Chr1g0340921 RchiOBHm_Chr1g0355301 RchiOBHm_Chr1g0355311 RchiOBHm_Chr1g0355331 RchiOBHm_Chr1g0355341 RchiOBHm_Chr2g0153901 RchiOBHm_Chr4g0384961 RchiOBHm_Chr4g0384991 RchiOBHm_Chr4g0393781 RchiOBHm_Chr4g0433801 RchiOBHm_Chr5g0024681 RchiOBHm_Chr5g0024711 RchiOBHm_Chr5g0024721 RchiOBHm_Chr5g0024791 RchiOBHm_Chr7g0177321 RchiOBHm_Chr7g0177351 RchiOBHm_Chr7g0177361
rosa_laevigata RLG00000001838 RLG00000005543 RLG00000010261 RLG00000028154 RLG00000028157 RLG00000028158 RLG00000028162 RLG00000030142 RLG00000032812 RLG00000032813 RLG00000032814
rosa_multiflora Rmu_co8235405.1_g000001 Rmu_co8395679.1_g000001 Rmu_co8439995.1_g000001 Rmu_sc0000999.1_g000011 Rmu_sc0001171.1_g000026 Rmu_sc0002766.1_g000009 Rmu_sc0003822.1_g000014 Rmu_sc0004135.1_g000001 Rmu_sc0004135.1_g000004 Rmu_sc0004135.1_g000006 Rmu_sc0004135.1_g000012 Rmu_sc0004135.1_g000017 Rmu_sc0004135.1_g000020 Rmu_sc0004637.1_g000001 Rmu_sc0005310.1_g000005 Rmu_sc0006151.1_g000002 Rmu_sc0007192.1_g000013 Rmu_sc0008633.1_g000002 Rmu_sc0020285.1_g000001
rosa_roxburghii Rroxscaffold_1G00054590 Rroxscaffold_2G00098180 Rroxscaffold_3G00234050 Rroxscaffold_3G00236820 Rroxscaffold_3G00275940 Rroxscaffold_4G00300500 Rroxscaffold_4G00300510 Rroxscaffold_5G00332950 Rroxscaffold_7G00188110
rosa_rugosa Rorug01G0051800 Rorug01G0051900 Rorug01G0052200 Rorug01G0052300 Rorug01G0052300 Rorug01G0246500 Rorug01G0246500 Rorug01G0246600 Rorug02G0411000 Rorug03G0298400 Rorug03G0298400 Rorug06G0404900 Rorug06G0405200 Rorug07G0209500 Rorug07G0227800
rosa_samantha Rh1AG066900 Rh1AG258800 Rh1AG259000 Rh1BG055200 Rh1BG055300 Rh1BG228400 Rh1BG228500 Rh1BG228600 Rh1BG228700 Rh1CG068300 Rh1CG242400 Rh1CG242600 Rh1CG242800 Rh1CG242900 Rh1CG243000 Rh1DG073000 Rh1DG256200 Rh1DG256300 Rh1DG256400 Rh1DG256500 Rh4AG002300 Rh4AG326300 Rh4BG001800 Rh4CG002200 Rh4DG001800 Rh5BG173900 Rh5CG190900 Rh5DG175500 Rh7AG004800 Rh7AG004900 Rh7AG349600 Rh7AG375800 Rh7BG004900 Rh7BG362800 Rh7CG004700 Rh7CG366800 Rh7CG394300 Rh7DG004600 Rh7DG004700 Rh7DG374000
rosa_wichuraiana Rw0G008010 Rw0G008840 Rw1G022840 Rw1G022850 Rw4G000710 Rw4G028240 Rw4G028290 Rw5G015960 Rw7G000400 Rw7G029670 Rw7G031540

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 545
AccB7I CCANNNNNTGG 1 cut(s) 614
AccI GTMKAC 2 cut(s) 8, 402
AciI CCGC 2 cut(s) 123, 474
AclWI GGATC 1 cut(s) 458
AcoI YGGCCR 1 cut(s) 120
AcsI RAATTY 2 cut(s) 483, 516
AcuI CTGAAG 2 cut(s) 314, 641
AcyI GRCGYC 1 cut(s) 5
AfiI CCNNNNNNNGG 5 cut(s) 68, 332, 473, 496, 614
AgsI TTSAA 3 cut(s) 133, 229, 553
AjnI CCWGG 1 cut(s) 300
AluBI AGCT 3 cut(s) 143, 638, 653
AluI AGCT 3 cut(s) 143, 638, 653
Alw21I GWGCWC 1 cut(s) 419
AlwI GGATC 1 cut(s) 458
AoxI GGCC 1 cut(s) 120
ApeKI GCWGC 1 cut(s) 350
ApoI RAATTY 2 cut(s) 483, 516
Asp700I GAANNNNTTC 1 cut(s) 441
AspLEI GCGC 1 cut(s) 451
AspS9I GGNCC 1 cut(s) 476
AsuC2I CCSGG 2 cut(s) 62, 492
AsuHPI GGTGA 1 cut(s) 58
AvaII GGWCC 1 cut(s) 476
Bbv12I GWGCWC 1 cut(s) 419
BbvI GCAGC 1 cut(s) 337
BccI CCATC 3 cut(s) 387, 543, 608
BciT130I CCWGG 1 cut(s) 302
BcnI CCSGG 2 cut(s) 62, 492
BfmI CTRYAG 1 cut(s) 9
BfoI RGCGCY 1 cut(s) 452
BglI GCCNNNNNGGC 1 cut(s) 305
BisI GCNGC 2 cut(s) 123, 351
BlsI GCNGC 2 cut(s) 124, 352
Bme1390I CCNGG 3 cut(s) 62, 302, 492
Bme18I GGWCC 1 cut(s) 476
BmgT120I GGNCC 1 cut(s) 476
BmrFI CCNGG 3 cut(s) 62, 302, 492
BmrI ACTGGG 1 cut(s) 639
BmuI ACTGGG 1 cut(s) 639
BpmI CTGGAG 3 cut(s) 123, 239, 323
BpuMI CCSGG 2 cut(s) 62, 492
BsaHI GRCGYC 1 cut(s) 5
BsaJI CCNNGG 3 cut(s) 300, 370, 490
BsaWI WCCGGW 1 cut(s) 68
Bsc4I CCNNNNNNNGG 5 cut(s) 68, 332, 473, 496, 614
Bse1I ACTGG 4 cut(s) 222, 410, 608, 645
BseBI CCWGG 1 cut(s) 302
BseDI CCNNGG 3 cut(s) 300, 370, 490
BseGI GGATG 2 cut(s) 268, 398
BseLI CCNNNNNNNGG 5 cut(s) 68, 332, 473, 496, 614
BseNI ACTGG 4 cut(s) 222, 410, 608, 645
BseXI GCAGC 1 cut(s) 337
BshFI GGCC 1 cut(s) 122
BsiHKAI GWGCWC 1 cut(s) 419
BsiSI CCGG 4 cut(s) 62, 69, 83, 492
BslI CCNNNNNNNGG 5 cut(s) 68, 332, 473, 496, 614
BsnI GGCC 1 cut(s) 122
Bsp1286I GDGCHC 1 cut(s) 419
Bsp143I GATC 3 cut(s) 28, 463, 582
BspACI CCGC 2 cut(s) 123, 474
BspANI GGCC 1 cut(s) 122
BspPI GGATC 1 cut(s) 458
BsrI ACTGG 4 cut(s) 222, 410, 608, 645
BssECI CCNNGG 3 cut(s) 300, 370, 490
BssMI GATC 3 cut(s) 28, 463, 582
BssNI GRCGYC 1 cut(s) 5
BssT1I CCWWGG 1 cut(s) 370
Bst2UI CCWGG 1 cut(s) 302
Bst4CI ACNGT 1 cut(s) 412
Bst6I CTCTTC 2 cut(s) 333, 450
BstACI GRCGYC 1 cut(s) 5
BstDEI CTNAG 1 cut(s) 521
BstEII GGTNACC 1 cut(s) 64
BstF5I GGATG 2 cut(s) 268, 398
BstH2I RGCGCY 1 cut(s) 452
BstHHI GCGC 1 cut(s) 451
BstKTI GATC 3 cut(s) 31, 466, 585
BstMBI GATC 3 cut(s) 28, 463, 582
BstMWI GCNNNNNNNGC 1 cut(s) 305
BstNI CCWGG 1 cut(s) 302
BstPI GGTNACC 1 cut(s) 64
BstSCI CCNGG 3 cut(s) 60, 300, 490
BstSFI CTRYAG 1 cut(s) 9
BstV1I GCAGC 1 cut(s) 337
BsuRI GGCC 1 cut(s) 122
BtgZI GCGATG 1 cut(s) 323
BtsCI GGATG 2 cut(s) 268, 398
BtsIMutI CAGTG 1 cut(s) 417
CfoI GCGC 1 cut(s) 451
Cfr13I GGNCC 1 cut(s) 476
CviAII CATG 4 cut(s) 26, 79, 427, 529
CviJI RGCY 8 cut(s) 122, 143, 299, 316, 350, 619, 638, 653
CviKI_1 RGCY 8 cut(s) 122, 143, 299, 316, 350, 619, 638, 653
DdeI CTNAG 1 cut(s) 521
DpnI GATC 3 cut(s) 30, 465, 584
DpnII GATC 3 cut(s) 28, 463, 582
EaeI YGGCCR 1 cut(s) 120
Eam1104I CTCTTC 2 cut(s) 333, 450
EarI CTCTTC 2 cut(s) 333, 450
Eco130I CCWWGG 1 cut(s) 370
Eco47I GGWCC 1 cut(s) 476
Eco57I CTGAAG 2 cut(s) 314, 641
Eco91I GGTNACC 1 cut(s) 64
EcoO65I GGTNACC 1 cut(s) 64
EcoRII CCWGG 1 cut(s) 300
EcoT14I CCWWGG 1 cut(s) 370
EcoT22I ATGCAT 2 cut(s) 27, 273
ErhI CCWWGG 1 cut(s) 370
FaeI CATG 4 cut(s) 29, 82, 430, 532
FatI CATG 4 cut(s) 25, 78, 426, 528
FblI GTMKAC 2 cut(s) 8, 402
Fnu4HI GCNGC 2 cut(s) 123, 351
FokI GGATG 2 cut(s) 275, 405
Fsp4HI GCNGC 2 cut(s) 123, 351
GlaI GCGC 1 cut(s) 450
GluI GCNGC 2 cut(s) 123, 351
GsuI CTGGAG 3 cut(s) 123, 239, 323
HaeII RGCGCY 1 cut(s) 452
HaeIII GGCC 1 cut(s) 122
HapII CCGG 4 cut(s) 62, 69, 83, 492
HhaI GCGC 1 cut(s) 451
Hin1I GRCGYC 1 cut(s) 5
Hin1II CATG 4 cut(s) 29, 82, 430, 532
Hin6I GCGC 1 cut(s) 449
HinP1I GCGC 1 cut(s) 449
HindIII AAGCTT 1 cut(s) 651
HinfI GANTC 2 cut(s) 549, 598
HpaII CCGG 4 cut(s) 62, 69, 83, 492
HphI GGTGA 1 cut(s) 58
Hpy166II GTNNAC 4 cut(s) 9, 212, 403, 568
Hpy188I TCNGA 1 cut(s) 206
Hpy188III TCNNGA 1 cut(s) 163
Hpy8I GTNNAC 4 cut(s) 9, 212, 403, 568
HpyAV CCTTC 3 cut(s) 66, 391, 616
HpyCH4III ACNGT 1 cut(s) 412
HpyCH4IV ACGT 1 cut(s) 294
HpyCH4V TGCA 6 cut(s) 25, 271, 353, 514, 532, 579
HpyF10VI GCNNNNNNNGC 1 cut(s) 305
HpyF3I CTNAG 1 cut(s) 521
HpySE526I ACGT 1 cut(s) 294
Hsp92I GRCGYC 1 cut(s) 5
Hsp92II CATG 4 cut(s) 29, 82, 430, 532
HspAI GCGC 1 cut(s) 449
Kzo9I GATC 3 cut(s) 28, 463, 582
Lsp1109I GCAGC 1 cut(s) 337
MaeII ACGT 1 cut(s) 294
MaeIII GTNAC 1 cut(s) 64
MalI GATC 3 cut(s) 30, 465, 584
MboI GATC 3 cut(s) 28, 463, 582
MboII GAAGA 2 cut(s) 320, 467
MfeI CAATTG 1 cut(s) 256
MhlI GDGCHC 1 cut(s) 419
MluCI AATT 7 cut(s) 93, 105, 256, 381, 483, 516, 574
MnlI CCTC 8 cut(s) 147, 241, 298, 306, 336, 340, 489, 641
Mph1103I ATGCAT 2 cut(s) 27, 273
MroXI GAANNNNTTC 1 cut(s) 441
MseI TTAA 1 cut(s) 655
MslI CAYNNNNRTG 1 cut(s) 425
MspI CCGG 4 cut(s) 62, 69, 83, 492
MspR9I CCNGG 3 cut(s) 62, 302, 492
MunI CAATTG 1 cut(s) 256
MvaI CCWGG 1 cut(s) 302
MwoI GCNNNNNNNGC 1 cut(s) 305
NciI CCSGG 2 cut(s) 62, 492
NdeII GATC 3 cut(s) 28, 463, 582
NlaIII CATG 4 cut(s) 29, 82, 430, 532
NmuCI GTSAC 1 cut(s) 64
NsiI ATGCAT 2 cut(s) 27, 273
PdmI GAANNNNTTC 1 cut(s) 441
PfeI GAWTC 2 cut(s) 549, 598
PflFI GACNNNGTC 1 cut(s) 62
PflMI CCANNNNNTGG 1 cut(s) 614
PkrI GCNGC 2 cut(s) 124, 352
PsiI TTATAA 1 cut(s) 545
Psp6I CCWGG 1 cut(s) 300
PspEI GGTNACC 1 cut(s) 64
PspGI CCWGG 1 cut(s) 300
PspPI GGNCC 1 cut(s) 476
PsyI GACNNNGTC 1 cut(s) 62
RseI CAYNNNNRTG 1 cut(s) 425
SaqAI TTAA 1 cut(s) 655
SatI GCNGC 2 cut(s) 123, 351
Sau3AI GATC 3 cut(s) 28, 463, 582
Sau96I GGNCC 1 cut(s) 476
ScrFI CCNGG 3 cut(s) 62, 302, 492
SduI GDGCHC 1 cut(s) 419
SfcI CTRYAG 1 cut(s) 9
SinI GGWCC 1 cut(s) 476
SmiMI CAYNNNNRTG 1 cut(s) 425
Sse9I AATT 7 cut(s) 93, 105, 256, 381, 483, 516, 574
SsiI CCGC 2 cut(s) 123, 474
SspI AATATT 1 cut(s) 440
StyD4I CCNGG 3 cut(s) 60, 300, 490
StyI CCWWGG 1 cut(s) 370
TaaI ACNGT 1 cut(s) 412
TaiI ACGT 1 cut(s) 297
TaqI TCGA 1 cut(s) 481
TasI AATT 7 cut(s) 93, 105, 256, 381, 483, 516, 574
TauI GCSGC 1 cut(s) 125
TfiI GAWTC 2 cut(s) 549, 598
Tru1I TTAA 1 cut(s) 655
Tru9I TTAA 1 cut(s) 655
TscAI CASTG 1 cut(s) 417
TseFI GTSAC 1 cut(s) 64
TseI GCWGC 1 cut(s) 350
Tsp45I GTSAC 1 cut(s) 64
TspDTI ATGAA 7 cut(s) 67, 105, 210, 293, 443, 517, 611
TspRI CASTG 1 cut(s) 417
Tth111I GACNNNGTC 1 cut(s) 62
Van91I CCANNNNNTGG 1 cut(s) 614
VpaK11BI GGWCC 1 cut(s) 476
XapI RAATTY 2 cut(s) 483, 516
XmiI GTMKAC 2 cut(s) 8, 402
XmnI GAANNNNTTC 1 cut(s) 441
Zsp2I ATGCAT 2 cut(s) 27, 273
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.