Rw5G015960

Cytochrome p450

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr5
OG0000192
Physical Location & Seq
Reverse (-)
19894659 .. 19896780
2122 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw5G015960.1

Sequence Viewer

Length: 1563 bp
ATGGAGTTGGAGTCGTTAATCCTTGTTGTGCTCGTCTTTGTTTCTTTTTACAATCTTTTTCGTTTGTTGAATGTAAATATTAATCCCAGGCTTAAGCGTGACAGTGGCACCAATACTGAGCAACTAGGGATTGAGCTACCACCAGGAAGCACCGGTTGGCCGATCATCGGTGAAACCCTCGAGTATCTACGCACAGCCAAACTGGGTGTCCCTGAAAAGTTCATAGCAGACAGGAGAAACAAACACTCGTCATCATTATCATGCAAGGTTTTCAAGACCTCTTTGCTACGCGAATCTATGGCCGTGATGTGCACTGCTGCGGGGAACAAATTTCTGTTTGCGAACGAGAACAAACTAGTCAAATCTTGGTGGCCTGCAAACCTTGAGAAGATCTTTGCCAACTCTAAGAATACAGACACCGCCCAAGAGTCGATTCGGTTGCGCAAAGTCTTGTCTCCGTTTCTCAAGCCTGATGCTCTTCGCAAGTACATAAGGGTCATGGACGAGGTCACCAAACAACATCTGGACATGTATTGGTCATCCTCCTCGTCCTCATCGGTTGACTACGTAAAAGAAGAGGATCAGAAAGTGATTATCAAAGTCCACCCATTAGCCAAGAAGTACACCTTCACTTTAGCATGTAGACTCTTCTTAAACCTTGAGGATCGAAAGCTAGCTGCCAAACTAGAGGAGTTAATTCGGCATATATCTTCGGGCTTCATTTCGCTGCCAATAGATTTGCCAGGCACGAAATTCAATCGAGCCATTAGAGCATCCAAGCAAATCAAGAAGGAAATTGAGGAGATCGTTAAGCAGAGGAGGATAGATCGAGATCTACATCTTCATCTTTTGGAGACTAGTAAGAGTAATTATCATCATCAAGATTTGTTGTCTAGTTTGCTCGTGGAGACATATAGCGATGGGAAAGAGCTGATGAAGGAATCAGACATTGCCAACAAGTTGTATGGTCTAATAGTCGGTGCTTATGACAATATAAGTACCACCTTGGTTTCGATTATCATGTTTCTATCGCAGCTTCCACATGTTTATGATGCCGTCCTTAAAGAGCAAATGGAGATTGCAGAATCAAAAGCAGAAGGGGAGTTACTGAACTGGGATGACTTGCAGAAGATGAAATATTCATGGAGCGTGGCATGTGAAGCGTTGAGATTGTTGCCACCAGTTCTCGGAACTTTTAGAGAGGCCATTACCGACTTTGCGTACGAAGGATACATGATTCCAAAAGGAATGAAGATACACTGGAACGTGTCTGCCACACATAAAAACCCGGAATACTTCCCGGATCCAGAGAAGTTCGACCCGTCAAGGTTCCACGGACAAGGACCAACTCCTTATTCATATGTTCCTTTTGGAGGAGGACCTCGAATGTGTCCAGGAAAAGAATATGCTCGGTTCAAGATATTGGTTTTCATGCATAATGTTGTCACTAGATTTAAATGGGAGATGGTTTTTCCAGACGAGAAAATGATCATGGACCCGGTTCTTATTCCTACAAAAGGACTTCCTATTCACCTTTTTCCTCATCAATCATCATCCTCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
p450

Protein Analysis

520

Amino Acids

59.61

Weight (kDa)

8.92

Isoelectric Point (pI)

48.4

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
p450 PF00067 47 - 507 7.9e-58 Cytochrome P450
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000192)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G36110 AT5G36110 AT5G36130 AT5G36140
fragaria_vesca FvH4_2g39160 FvH4_3g15030 FvH4_3g38021 FvH4_3g38080 FvH4_4g00250 FvH4_4g01400 FvH4_5g18260 FvH4_5g18260 FvH4_5g29490 FvH4_7g03260 FvH4_7g12550 FvH4_7g12560 FvH4_7g14310 FvH4_7g14330
malus_domestica MD04G1002500.v1.1 MD11G1006100.v1.1 MD11G1157200.v1.1 MD11G1157300.v1.1 MD13G1116400.v1.1 MD13G1219600.v1.1 MD13G1284800.v1.1 MD16G1278300.v1.1 MD17G1219500.v1.1
prunus_persica Prupe.1G002500_v2.0.a1 Prupe.6G120400_v2.0.a1
pyrus_communis pycom04g00270 pycom10g20130 pycom11g12750 pycom16g24900 pycom17g22420 pycom420g00590
rosa_chinensis RchiOBHm_Chr1g0325391 RchiOBHm_Chr1g0340921 RchiOBHm_Chr1g0355301 RchiOBHm_Chr1g0355311 RchiOBHm_Chr1g0355331 RchiOBHm_Chr1g0355341 RchiOBHm_Chr2g0153901 RchiOBHm_Chr4g0384961 RchiOBHm_Chr4g0384991 RchiOBHm_Chr4g0393781 RchiOBHm_Chr4g0433801 RchiOBHm_Chr5g0024681 RchiOBHm_Chr5g0024711 RchiOBHm_Chr5g0024721 RchiOBHm_Chr5g0024791 RchiOBHm_Chr7g0177321 RchiOBHm_Chr7g0177351 RchiOBHm_Chr7g0177361
rosa_laevigata RLG00000001838 RLG00000005543 RLG00000010261 RLG00000028154 RLG00000028157 RLG00000028158 RLG00000028162 RLG00000030142 RLG00000032812 RLG00000032813 RLG00000032814
rosa_multiflora Rmu_co8235405.1_g000001 Rmu_co8395679.1_g000001 Rmu_co8439995.1_g000001 Rmu_sc0000999.1_g000011 Rmu_sc0001171.1_g000026 Rmu_sc0002766.1_g000009 Rmu_sc0003822.1_g000014 Rmu_sc0004135.1_g000001 Rmu_sc0004135.1_g000004 Rmu_sc0004135.1_g000006 Rmu_sc0004135.1_g000012 Rmu_sc0004135.1_g000017 Rmu_sc0004135.1_g000020 Rmu_sc0004637.1_g000001 Rmu_sc0005310.1_g000005 Rmu_sc0006151.1_g000002 Rmu_sc0007192.1_g000013 Rmu_sc0008633.1_g000002 Rmu_sc0020285.1_g000001
rosa_roxburghii Rroxscaffold_1G00054590 Rroxscaffold_2G00098180 Rroxscaffold_3G00234050 Rroxscaffold_3G00236820 Rroxscaffold_3G00275940 Rroxscaffold_4G00300500 Rroxscaffold_4G00300510 Rroxscaffold_5G00332950 Rroxscaffold_7G00188110
rosa_rugosa Rorug01G0051800 Rorug01G0051900 Rorug01G0052200 Rorug01G0052300 Rorug01G0052300 Rorug01G0246500 Rorug01G0246500 Rorug01G0246600 Rorug02G0411000 Rorug03G0298400 Rorug03G0298400 Rorug06G0404900 Rorug06G0405200 Rorug07G0209500 Rorug07G0227800
rosa_samantha Rh1AG066900 Rh1AG258800 Rh1AG259000 Rh1BG055200 Rh1BG055300 Rh1BG228400 Rh1BG228500 Rh1BG228600 Rh1BG228700 Rh1CG068300 Rh1CG242400 Rh1CG242600 Rh1CG242800 Rh1CG242900 Rh1CG243000 Rh1DG073000 Rh1DG256200 Rh1DG256300 Rh1DG256400 Rh1DG256500 Rh4AG002300 Rh4AG326300 Rh4BG001800 Rh4CG002200 Rh4DG001800 Rh5BG173900 Rh5CG190900 Rh5DG175500 Rh7AG004800 Rh7AG004900 Rh7AG349600 Rh7AG375800 Rh7BG004900 Rh7BG362800 Rh7CG004700 Rh7CG366800 Rh7CG394300 Rh7DG004600 Rh7DG004700 Rh7DG374000
rosa_wichuraiana Rw0G008010 Rw0G008840 Rw1G022840 Rw1G022850 Rw4G000710 Rw4G028240 Rw4G028290 Rw5G015960 Rw7G000400 Rw7G029670 Rw7G031540

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 443
AccB1I GGYRCC 1 cut(s) 107
AccI GTMKAC 1 cut(s) 643
AccII CGCG 1 cut(s) 291
AciI CCGC 2 cut(s) 320, 420
AclWI GGATC 4 cut(s) 588, 672, 1298, 1311
AcoI YGGCCR 2 cut(s) 158, 300
AcsI RAATTY 2 cut(s) 329, 752
AfaI GTAC 4 cut(s) 488, 623, 1000, 1223
AfiI CCNNNNNNNGG 4 cut(s) 167, 1187, 1373, 1517
AflII CTTAAG 1 cut(s) 92
AflIII ACRYGT 3 cut(s) 528, 1042, 1266
AgeI ACCGGT 1 cut(s) 152
AgsI TTSAA 4 cut(s) 70, 274, 757, 1417
AhlI ACTAGT 2 cut(s) 355, 857
AjnI CCWGG 4 cut(s) 86, 142, 742, 1393
AjuI GAANNNNNNNTTGG 6 cut(s) 139, 171, 417, 449, 1339, 1371
AluBI AGCT 5 cut(s) 136, 673, 677, 931, 1036
AluI AGCT 5 cut(s) 136, 673, 677, 931, 1036
Alw21I GWGCWC 2 cut(s) 33, 314
Alw26I GTCTC 3 cut(s) 459, 848, 902
Alw44I GTGCAC 1 cut(s) 310
AlwI GGATC 4 cut(s) 588, 672, 1298, 1311
Ama87I CYCGRG 1 cut(s) 179
AoxI GGCC 4 cut(s) 158, 300, 371, 1203
ApaLI GTGCAC 1 cut(s) 310
ApeKI GCWGC 4 cut(s) 317, 677, 727, 1033
ApoI RAATTY 2 cut(s) 329, 752
ArsI GACNNNNNNTTYG 2 cut(s) 192, 224
AseI ATTAAT 1 cut(s) 81
AsiGI ACCGGT 1 cut(s) 152
AspLEI GCGC 1 cut(s) 444
AspS9I GGNCC 3 cut(s) 1343, 1379, 1495
AsuC2I CCSGG 3 cut(s) 1289, 1301, 1499
AsuHPI GGTGA 3 cut(s) 182, 502, 1523
AsuNHI GCTAGC 1 cut(s) 673
AvaI CYCGRG 1 cut(s) 179
AvaII GGWCC 3 cut(s) 1343, 1379, 1495
BaeGI GKGCMC 1 cut(s) 314
BamHI GGATCC 1 cut(s) 1303
BanI GGYRCC 1 cut(s) 107
BarI GAAGNNNNNNTAC 2 cut(s) 1089, 1121
BauI CACGAG 1 cut(s) 902
Bbv12I GWGCWC 2 cut(s) 33, 314
BbvI GCAGC 4 cut(s) 304, 664, 714, 1045
BccI CCATC 2 cut(s) 914, 1459
BceAI ACGGC 2 cut(s) 287, 1040
BcgI CGANNNNNNTGC 2 cut(s) 421, 455
BciT130I CCWGG 4 cut(s) 88, 144, 744, 1395
BciVI GTATCC 1 cut(s) 1223
BclI TGATCA 1 cut(s) 1488
BcnI CCSGG 3 cut(s) 1289, 1301, 1499
BcoDI GTCTC 3 cut(s) 459, 848, 902
BcuI ACTAGT 2 cut(s) 355, 857
BfaI CTAG 7 cut(s) 125, 356, 674, 686, 858, 894, 1449
BfrI CTTAAG 1 cut(s) 92
BfuI GTATCC 1 cut(s) 1223
BglII AGATCT 2 cut(s) 390, 832
BisI GCNGC 4 cut(s) 318, 678, 728, 1034
BlsI GCNGC 4 cut(s) 319, 679, 729, 1035
Bme1390I CCNGG 7 cut(s) 88, 144, 744, 1289, 1301, 1395, 1499
Bme18I GGWCC 3 cut(s) 1343, 1379, 1495
BmeT110I CYCGRG 1 cut(s) 179
BmgT120I GGNCC 3 cut(s) 1343, 1379, 1495
BmiI GGNNCC 4 cut(s) 109, 1305, 1331, 1497
BmrFI CCNGG 7 cut(s) 88, 144, 744, 1289, 1301, 1395, 1499
BmrI ACTGGG 2 cut(s) 212, 1123
BmsI GCATC 3 cut(s) 463, 782, 1042
BmtI GCTAGC 1 cut(s) 677
BmuI ACTGGG 2 cut(s) 212, 1123
BpuEI CTTGAG 3 cut(s) 404, 449, 680
BpuMI CCSGG 3 cut(s) 1289, 1301, 1499
BsaAI YACGTR 1 cut(s) 568
BsaBI GATNNNNATC 2 cut(s) 831, 837
BsaJI CCNNGG 3 cut(s) 86, 1005, 1333
BsaWI WCCGGW 1 cut(s) 152
BsaXI ACNNNNNCTCC 1 cut(s) 26
Bsc4I CCNNNNNNNGG 4 cut(s) 167, 1187, 1373, 1517
Bse118I RCCGGY 1 cut(s) 152
Bse1I ACTGG 4 cut(s) 207, 1118, 1181, 1265
Bse3DI GCAATG 1 cut(s) 948
Bse8I GATNNNNATC 2 cut(s) 831, 837
BseBI CCWGG 4 cut(s) 88, 144, 744, 1395
BseDI CCNNGG 3 cut(s) 86, 1005, 1333
BseGI GGATG 4 cut(s) 539, 773, 1123, 1553
BseJI GATNNNNATC 2 cut(s) 831, 837
BseLI CCNNNNNNNGG 4 cut(s) 167, 1187, 1373, 1517
BseMI GCAATG 1 cut(s) 948
BseMII CTCAG 1 cut(s) 108
BseNI ACTGG 4 cut(s) 207, 1118, 1181, 1265
BseRI GAGGAG 5 cut(s) 535, 704, 815, 832, 1389
BseSI GKGCMC 1 cut(s) 314
BseXI GCAGC 4 cut(s) 304, 664, 714, 1045
Bsh1236I CGCG 1 cut(s) 291
BshFI GGCC 4 cut(s) 160, 302, 373, 1205
BshNI GGYRCC 1 cut(s) 107
BshTI ACCGGT 1 cut(s) 152
BsiHKAI GWGCWC 2 cut(s) 33, 314
BsiHKCI CYCGRG 1 cut(s) 179
BsiSI CCGG 4 cut(s) 153, 1289, 1301, 1499
BsiWI CGTACG 1 cut(s) 1221
BslFI GGGAC 1 cut(s) 194
BslI CCNNNNNNNGG 4 cut(s) 167, 1187, 1373, 1517
BsmAI GTCTC 3 cut(s) 459, 848, 902
BsmFI GGGAC 1 cut(s) 194
BsnI GGCC 4 cut(s) 160, 302, 373, 1205
BsoBI CYCGRG 1 cut(s) 179
Bsp1286I GDGCHC 2 cut(s) 33, 314
Bsp143I GATC 9 cut(s) 162, 390, 580, 664, 804, 826, 832, 1303, 1488
BspACI CCGC 2 cut(s) 320, 420
BspANI GGCC 4 cut(s) 160, 302, 373, 1205
BspCNI CTCAG 1 cut(s) 109
BspFNI CGCG 1 cut(s) 291
BspHI TCATGA 1 cut(s) 1559
BspLI GGNNCC 4 cut(s) 109, 1305, 1331, 1497
BspOI GCTAGC 1 cut(s) 677
BspPI GGATC 4 cut(s) 588, 672, 1298, 1311
BspQI GCTCTTC 1 cut(s) 483
BspT107I GGYRCC 1 cut(s) 107
BspTI CTTAAG 1 cut(s) 92
BsrDI GCAATG 1 cut(s) 948
BsrFI RCCGGY 1 cut(s) 152
BsrI ACTGG 4 cut(s) 207, 1118, 1181, 1265
BssAI RCCGGY 1 cut(s) 152
BssECI CCNNGG 3 cut(s) 86, 1005, 1333
BssMI GATC 9 cut(s) 162, 390, 580, 664, 804, 826, 832, 1303, 1488
BssSI CACGAG 1 cut(s) 902
BssT1I CCWWGG 1 cut(s) 1005
Bst2BI CACGAG 1 cut(s) 902
Bst2UI CCWGG 4 cut(s) 88, 144, 744, 1395
Bst4CI ACNGT 1 cut(s) 104
Bst6I CTCTTC 3 cut(s) 483, 570, 653
BstAFI CTTAAG 1 cut(s) 92
BstBAI YACGTR 1 cut(s) 568
BstC8I GCNNGC 2 cut(s) 375, 675
BstDEI CTNAG 2 cut(s) 117, 405
BstDSI CCRYGG 1 cut(s) 1333
BstEII GGTNACC 1 cut(s) 508
BstENI CCTNNNNNAGG 2 cut(s) 1371, 1515
BstF5I GGATG 4 cut(s) 539, 773, 1123, 1553
BstFNI CGCG 1 cut(s) 291
BstHHI GCGC 1 cut(s) 444
BstKTI GATC 9 cut(s) 165, 393, 583, 667, 807, 829, 835, 1306, 1491
BstMAI GTCTC 3 cut(s) 459, 848, 902
BstMBI GATC 9 cut(s) 162, 390, 580, 664, 804, 826, 832, 1303, 1488
BstMWI GCNNNNNNNGC 2 cut(s) 770, 1160
BstNI CCWGG 4 cut(s) 88, 144, 744, 1395
BstNSI RCATGY 4 cut(s) 532, 642, 1046, 1158
BstPI GGTNACC 1 cut(s) 508
BstSCI CCNGG 7 cut(s) 86, 142, 742, 1287, 1299, 1393, 1497
BstSLI GKGCMC 1 cut(s) 314
BstSNI TACGTA 1 cut(s) 568
BstUI CGCG 1 cut(s) 291
BstV1I GCAGC 4 cut(s) 304, 664, 714, 1045
BstX2I RGATCY 3 cut(s) 390, 832, 1303
BstYI RGATCY 3 cut(s) 390, 832, 1303
BsuI GTATCC 1 cut(s) 1223
BsuRI GGCC 4 cut(s) 160, 302, 373, 1205
BtgI CCRYGG 1 cut(s) 1333
BtgZI GCGATG 1 cut(s) 933
BtsCI GGATG 4 cut(s) 539, 773, 1123, 1553
BtsI GCAGTG 1 cut(s) 312
BtsIMutI CAGTG 3 cut(s) 109, 312, 1258
Cac8I GCNNGC 2 cut(s) 375, 675
CciI TCATGA 1 cut(s) 1559
CfoI GCGC 1 cut(s) 444
Cfr10I RCCGGY 1 cut(s) 152
Cfr13I GGNCC 3 cut(s) 1343, 1379, 1495
Csp6I GTAC 4 cut(s) 487, 622, 999, 1222
CspAI ACCGGT 1 cut(s) 152
CviQI GTAC 4 cut(s) 487, 622, 999, 1222
DdeI CTNAG 2 cut(s) 117, 405
DpnI GATC 9 cut(s) 164, 392, 582, 666, 806, 828, 834, 1305, 1490
DpnII GATC 9 cut(s) 162, 390, 580, 664, 804, 826, 832, 1303, 1488
DraI TTTAAA 1 cut(s) 1456
EaeI YGGCCR 2 cut(s) 158, 300
Eam1104I CTCTTC 3 cut(s) 483, 570, 653
EarI CTCTTC 3 cut(s) 483, 570, 653
Eco105I TACGTA 1 cut(s) 568
Eco130I CCWWGG 1 cut(s) 1005
Eco47I GGWCC 3 cut(s) 1343, 1379, 1495
Eco88I CYCGRG 1 cut(s) 179
Eco91I GGTNACC 1 cut(s) 508
EcoNI CCTNNNNNAGG 2 cut(s) 1371, 1515
EcoO109I RGGNCCY 1 cut(s) 1379
EcoO65I GGTNACC 1 cut(s) 508
EcoRII CCWGG 4 cut(s) 86, 142, 742, 1393
EcoT14I CCWWGG 1 cut(s) 1005
EcoT22I ATGCAT 1 cut(s) 1437
ErhI CCWWGG 1 cut(s) 1005
FalI AAGNNNNNCTT 2 cut(s) 611, 643
FaqI GGGAC 1 cut(s) 194
FauI CCCGC 1 cut(s) 313
FauNDI CATATG 1 cut(s) 1360
FbaI TGATCA 1 cut(s) 1488
FblI GTMKAC 1 cut(s) 643
Fnu4HI GCNGC 4 cut(s) 318, 678, 728, 1034
FokI GGATG 4 cut(s) 526, 760, 1130, 1540
Fsp4HI GCNGC 4 cut(s) 318, 678, 728, 1034
FspBI CTAG 7 cut(s) 125, 356, 674, 686, 858, 894, 1449
FspI TGCGCA 1 cut(s) 443
GlaI GCGC 1 cut(s) 443
GluI GCNGC 4 cut(s) 318, 678, 728, 1034
HaeIII GGCC 4 cut(s) 160, 302, 373, 1205
HapII CCGG 4 cut(s) 153, 1289, 1301, 1499
HhaI GCGC 1 cut(s) 444
Hin6I GCGC 1 cut(s) 442
HinP1I GCGC 1 cut(s) 442
HincII GTYRAC 1 cut(s) 562
HindII GTYRAC 1 cut(s) 562
HinfI GANTC 8 cut(s) 11, 293, 428, 433, 645, 941, 1085, 1237
HpaII CCGG 4 cut(s) 153, 1289, 1301, 1499
HphI GGTGA 3 cut(s) 182, 502, 1523
Hpy166II GTNNAC 5 cut(s) 312, 562, 604, 624, 644
Hpy188I TCNGA 3 cut(s) 585, 946, 1190
Hpy188III TCNNGA 9 cut(s) 274, 524, 787, 830, 881, 1307, 1417, 1475, 1560
Hpy8I GTNNAC 5 cut(s) 312, 562, 604, 624, 644
HpyAV CCTTC 5 cut(s) 637, 784, 931, 1091, 1220
HpyCH4III ACNGT 1 cut(s) 104
HpyCH4IV ACGT 2 cut(s) 567, 1266
HpyCH4V TGCA 6 cut(s) 264, 312, 377, 1082, 1126, 1435
HpyF10VI GCNNNNNNNGC 2 cut(s) 770, 1160
HpyF3I CTNAG 2 cut(s) 117, 405
HpySE526I ACGT 2 cut(s) 567, 1266
HspAI GCGC 1 cut(s) 442
Ksp22I TGATCA 1 cut(s) 1488
Kzo9I GATC 9 cut(s) 162, 390, 580, 664, 804, 826, 832, 1303, 1488
LguI GCTCTTC 1 cut(s) 483
LmnI GCTCC 1 cut(s) 1146
Lsp1109I GCAGC 4 cut(s) 304, 664, 714, 1045
LweI GCATC 3 cut(s) 463, 782, 1042
MaeI CTAG 7 cut(s) 125, 356, 674, 686, 858, 894, 1449
MaeII ACGT 2 cut(s) 567, 1266
MaeIII GTNAC 4 cut(s) 98, 508, 1104, 1444
MalI GATC 9 cut(s) 164, 392, 582, 666, 806, 828, 834, 1305, 1490
MboI GATC 9 cut(s) 162, 390, 580, 664, 804, 826, 832, 1303, 1488
MboII GAAGA 8 cut(s) 400, 470, 587, 640, 702, 833, 1141, 1264
MflI RGATCY 3 cut(s) 390, 832, 1303
MhlI GDGCHC 2 cut(s) 33, 314
MluCI AATT 5 cut(s) 329, 696, 752, 795, 868
MlyI GAGTC 3 cut(s) 20, 437, 639
Mph1103I ATGCAT 1 cut(s) 1437
MseI TTAA 8 cut(s) 17, 81, 93, 653, 695, 810, 1062, 1455
MslI CAYNNNNRTG 3 cut(s) 259, 1047, 1558
MspCI CTTAAG 1 cut(s) 92
MspI CCGG 4 cut(s) 153, 1289, 1301, 1499
MspR9I CCNGG 7 cut(s) 88, 144, 744, 1289, 1301, 1395, 1499
MvaI CCWGG 4 cut(s) 88, 144, 744, 1395
MvnI CGCG 1 cut(s) 291
MwoI GCNNNNNNNGC 2 cut(s) 770, 1160
NciI CCSGG 3 cut(s) 1289, 1301, 1499
NdeI CATATG 1 cut(s) 1360
NdeII GATC 9 cut(s) 162, 390, 580, 664, 804, 826, 832, 1303, 1488
NheI GCTAGC 1 cut(s) 673
NlaIV GGNNCC 4 cut(s) 109, 1305, 1331, 1497
NmuCI GTSAC 3 cut(s) 98, 508, 1444
NsbI TGCGCA 1 cut(s) 443
NsiI ATGCAT 1 cut(s) 1437
NspI RCATGY 4 cut(s) 532, 642, 1046, 1158
PaeR7I CTCGAG 1 cut(s) 179
PagI TCATGA 1 cut(s) 1559
PciI ACATGT 2 cut(s) 528, 1042
PciSI GCTCTTC 1 cut(s) 483
PfeI GAWTC 5 cut(s) 293, 433, 941, 1085, 1237
Pfl23II CGTACG 1 cut(s) 1221
PflFI GACNNNGTC 1 cut(s) 506
PfoI TCCNGGA 2 cut(s) 1299, 1393
PinAI ACCGGT 1 cut(s) 152
PkrI GCNGC 4 cut(s) 319, 679, 729, 1035
PleI GAGTC 3 cut(s) 19, 436, 639
PpsI GAGTC 3 cut(s) 19, 436, 639
Ppu21I YACGTR 1 cut(s) 568
PpuMI RGGWCCY 1 cut(s) 1379
PscI ACATGT 2 cut(s) 528, 1042
PshBI ATTAAT 1 cut(s) 81
Psp5II RGGWCCY 1 cut(s) 1379
Psp6I CCWGG 4 cut(s) 86, 142, 742, 1393
PspEI GGTNACC 1 cut(s) 508
PspGI CCWGG 4 cut(s) 86, 142, 742, 1393
PspLI CGTACG 1 cut(s) 1221
PspN4I GGNNCC 4 cut(s) 109, 1305, 1331, 1497
PspPI GGNCC 3 cut(s) 1343, 1379, 1495
PspPPI RGGWCCY 1 cut(s) 1379
PspXI VCTCGAGB 1 cut(s) 179
PsuI RGATCY 3 cut(s) 390, 832, 1303
PsyI GACNNNGTC 1 cut(s) 506
RsaI GTAC 4 cut(s) 488, 623, 1000, 1223
RsaNI GTAC 4 cut(s) 487, 622, 999, 1222
RseI CAYNNNNRTG 3 cut(s) 259, 1047, 1558
SapI GCTCTTC 1 cut(s) 483
SaqAI TTAA 8 cut(s) 17, 81, 93, 653, 695, 810, 1062, 1455
SatI GCNGC 4 cut(s) 318, 678, 728, 1034
Sau3AI GATC 9 cut(s) 162, 390, 580, 664, 804, 826, 832, 1303, 1488
Sau96I GGNCC 3 cut(s) 1343, 1379, 1495
SchI GAGTC 3 cut(s) 20, 437, 639
ScrFI CCNGG 7 cut(s) 88, 144, 744, 1289, 1301, 1395, 1499
SduI GDGCHC 2 cut(s) 33, 314
SfaNI GCATC 3 cut(s) 463, 782, 1042
Sfr274I CTCGAG 1 cut(s) 179
SinI GGWCC 3 cut(s) 1343, 1379, 1495
SlaI CTCGAG 1 cut(s) 179
SmiI ATTTAAAT 1 cut(s) 1456
SmiMI CAYNNNNRTG 3 cut(s) 259, 1047, 1558
SmlI CTYRAG 5 cut(s) 92, 179, 383, 464, 659
SmoI CTYRAG 5 cut(s) 92, 179, 383, 464, 659
SnaBI TACGTA 1 cut(s) 568
SpeI ACTAGT 2 cut(s) 355, 857
Sse9I AATT 5 cut(s) 329, 696, 752, 795, 868
SsiI CCGC 2 cut(s) 320, 420
SspI AATATT 2 cut(s) 79, 1139
SspMI CTAG 7 cut(s) 125, 356, 674, 686, 858, 894, 1449
StyD4I CCNGG 7 cut(s) 86, 142, 742, 1287, 1299, 1393, 1497
StyI CCWWGG 1 cut(s) 1005
SwaI ATTTAAAT 1 cut(s) 1456
TaaI ACNGT 1 cut(s) 104
TaiI ACGT 2 cut(s) 570, 1269
TaqI TCGA 8 cut(s) 180, 431, 667, 760, 829, 1013, 1317, 1384
TasI AATT 5 cut(s) 329, 696, 752, 795, 868
TatI WGTACW 2 cut(s) 486, 621
TfiI GAWTC 5 cut(s) 293, 433, 941, 1085, 1237
Tru1I TTAA 8 cut(s) 17, 81, 93, 653, 695, 810, 1062, 1455
Tru9I TTAA 8 cut(s) 17, 81, 93, 653, 695, 810, 1062, 1455
TscAI CASTG 3 cut(s) 109, 319, 1265
TseFI GTSAC 3 cut(s) 98, 508, 1444
TseI GCWGC 4 cut(s) 317, 677, 727, 1033
Tsp45I GTSAC 3 cut(s) 98, 508, 1444
TspDTI ATGAA 9 cut(s) 211, 709, 833, 950, 1131, 1148, 1265, 1347, 1420
TspGWI ACGGA 2 cut(s) 447, 1350
TspRI CASTG 3 cut(s) 109, 319, 1265
Tth111I GACNNNGTC 1 cut(s) 506
Vha464I CTTAAG 1 cut(s) 92
VneI GTGCAC 1 cut(s) 310
VpaK11BI GGWCC 3 cut(s) 1343, 1379, 1495
VspI ATTAAT 1 cut(s) 81
XagI CCTNNNNNAGG 2 cut(s) 1371, 1515
XapI RAATTY 2 cut(s) 329, 752
XceI RCATGY 4 cut(s) 532, 642, 1046, 1158
XcmI CCANNNNNNNNNTGG 1 cut(s) 520
XhoI CTCGAG 1 cut(s) 179
XmiI GTMKAC 1 cut(s) 643
XspI CTAG 7 cut(s) 125, 356, 674, 686, 858, 894, 1449
Zsp2I ATGCAT 1 cut(s) 1437
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.